KR20180058251A - Functional food composition for maintenance of mineral balance in body and detoxification heavy metal using grub - Google Patents

Abstract

The present invention relates to a functional food composition for maintenance of mineral balance in a body, absorption prevention and external discharge of heavy metal and the like using cicada larva to be effective in detoxification, wherein the food composition constitutes a dried yeast comprising at least one sulfur-containing amino acid selected from a cicada larva powder, methionine, cysteine and cysteine, tocopherol, pyridoxine, ascorbic acid, calcium, magnesium, zinc and selenium.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a functional food composition for maintaining mineral balance in a body using slugs, preventing in vivo absorption of heavy metals,

The present invention relates to a functional food composition which is produced by using slugs and is effective for detoxification such as maintenance of a balance in the body, inhibition of absorption of heavy metals by the body, and excretion in the body.

The development of the industrial society has made life convenient, while the environmental pollution has become a threat to human life environment. Among them, heavy metal contamination of soil and water quality is transferred to the body through the food chain and accumulation may cause acute or chronic poisoning. If heavy metals form organic or inorganic compounds depending on the kind, it becomes easy to move in the living body. The heavy metals contained in fine dusts or dusts can be absorbed into the human body through the respiratory tract and cause more problems.

These heavy metals are not easily released when they enter the living body, and when the accumulation amount increases, they cause poisoning such as weight loss, anemia, biochemical and morphological changes of organs, brain damage and the like, They act competitively in the intestinal absorption stage and reduce these contents in the tissue. In addition, some toxic metals react with body tissues even at relatively low concentrations and gradually exhibit toxic effects in the body. In particular, once the biological half-life is long, it is impossible to cure once it is poisoned.

For example, cadmium (Cd) is one of the most toxic substances among heavy metals and is classified as an environmental heavy metal, a carcinogen causing cancer in humans (Satoh, M., et al., Tohoku J. Exp. Med , 196, pp23-32, 2002). Epidemiologic studies indicate that cadmium accumulates in target organs after a long period of ingestion even with small amounts of cadmium, resulting in adverse health effects.

The total amount of cadmium consumed by humans for a lifetime is about 2000 mg (Klaassen, CD, Casarett and Doull'sToxociology, McGraw-Hill Medical Publishing Division, New York, pp. 822-826, 2001) Cadmium can kill organelles in accumulated tissues, resulting in organ damage (Falck, FYJr., Et al., Am. J. Ind. Med., 4, pp541 -549, 1983). Cadmium accumulates primarily in the liver and kidney, and chronic accumulation of cadmium in low doses causes pulmonary, hepatic, and renal tubular disease.

The morphological changes of cells induced by cadmium exposure include cellular degeneration, inflammatory response, and fibrosis. The pathological mechanisms of cadmium-induced tissue injury are related to cell necrosis and high cell turnover rates (Sauer, J.M., et al., Toxicology, 121, pp155-164, 1997).

In general, low doses of toxic substances tend to cause apoptosis, but high levels of toxin exposure later lead to cellular necrosis (Raffray, M., et al., Pharmacol. Ther., 75, 153 -177, 1997). Degradation of the protein by apoptosis stimulates the release of protein-bound Bad, promotes the translocation of the bad to the mitochondria, and interacts with BclXL in the mitochondria to increase cell death (Nomura, M., et al., J. Biol. Chem., 278, pp2058-2065, 2003).

As a result of this social background, the efficacy of fibrin, protein, selenium and trace elements has been studied as a part of research to alleviate the symptoms of lead and cadmium poisoning. Tea and green tea catechin have improved renal function due to cadmium poisoning, And other studies have been reported.

In addition, when harmful substances are introduced into the body, biotransformation proceeds in order to rapidly excrete harmful substances in the body. As a result, harmful substances are excreted out of the body through such action. This metabolic process is largely divided into two stages: foreign substances such as environmental pollutants or drugs, which are absorbed into the body, are excreted in the liver via phase 1 or phase 2 reactions or both. The phase 1 enzyme contains cytochrome P450 and converts it to a highly reactive compound by changing it to have reactive electrons through oxidation, reduction and hydrolysis of hydrophobic compounds. Phase 2 enzymes include glutathione S-transferase, UDP-glucuronosyl transferase, and guinone reductase, which convert high-metabolism substances to stable substances and help them to excrete easily (Liska DJ. Alter med. rev 3: 187-198,1998).

Recently, there have been many studies on the correlation between cancer and gulcosinolates, a sulfur-containing substance of cruciferous vegetables such as cauliflower, cabbage, kale and broccoli, as potential cancer-preventing substances. As a result, gulcosinolates have been shown to inhibit cancer .

Thus, studies on the anticancer activity related to the induction of the phase 1 reaction or the enzyme activity of the phase 2 are active, but the research on the detoxifying action of heavy metal substances and the scientific data are insufficient and the accumulation and release of heavy metals can be induced However, there are still few studies on functional foods.

It is also important to maintain the body's mineral balance in order to maintain immune function, maintain cell activity, and prevent disease. Research on food that maintains the balance while correcting the destructed balance is also weak.

[Patent Document 1] Korean Patent Publication No. 2000-0004499 [Patent Document 2] Korean Patent Publication No. 2003-0079205 [Patent Document 3] Korean Patent Publication No. 2004-0107853 [Patent Document 4] Korean Patent Publication No. 2004-0107852 [Document 5] Korean Patent Publication No. 2005-0036093

Accordingly, the present invention is concerned with a composition capable of alleviating harm to the body's mineral balance and toxic heavy metals such as lead and cadmium toxicity, and has a remarkable effect of maintaining the body's mineral balance and heavy metal decontamination effect when using the slugs The present invention can be used as a health food for maintaining a balance in the body and detoxifying heavy metals.

Accordingly, a problem to be solved by the present invention is to provide a functional food composition for sustaining an in-vivo mineral balance using slugs, preventing in-vivo absorption of heavy metals, and excretion in vitro.

According to an aspect of the present invention,

20 to 30% by weight of dried slugs,

55 to 70% by weight of at least one sulfur-containing amino acid selected from the group consisting of methionine, cysteine and cystine,

Tocopherol 0.5 to 1.0% by weight,

0.5 to 1.0% by weight of pyridoxine,

2.5 to 3.5% by weight of ascorbic acid,

1.0 to 2.0% by weight of calcium,

0.5 to 1.0% by weight of magnesium,

0.5 to 1.0% by weight of zinc, and

Selenium-containing dry yeast 2.5-3.5 wt%

A functional food composition for maintaining balance in the body, preventing in vivo absorption of heavy metals, and excretion in vitro.

In the functional food composition according to the present invention,

600 mg of the dried slugs powder,

1500 mg of any one or more sulfur-containing amino acids selected from the group consisting of methionine, cysteine and cystine,

Tocopherol 25 mg,

Pyridoxine 17 mg,

Ascorbic acid 85 mg,

Calcium 40 mg,

Magnesium 20 mg,

10 mg of zinc, and

Selenium-containing dry yeast 79 mg

.

In the functional food composition according to the present invention,

The dried slug powder

A method in which slugs are fasted and then lyophilized to prepare powder

Slugs are slaughtered, boiled in boiling water for 5 to 20 seconds and dried at 40 to 60 ° C to prepare a powder

It is preferable to be produced.

In the functional food composition according to the present invention,

In order to produce the dried slugs, it is preferable to use water which leaves the gugija when draining the slugs.

The functional food of the present invention was confirmed to be excellent in the excretion of heavy metals as a result of checking the degree of discharge of heavy metals in the blood, so that it could be used as a functional food for new treatment and prevention of diseases caused by heavy metal poisoning, When it is in a normal state, it maintains it and it is useful for maintenance of cell activity and immunity.

Hereinafter, the present invention will be described in detail so as to be easily carried out by those skilled in the art.

The functional food composition according to the present invention comprises

20 to 30% by weight of dried slugs,

55 to 70% by weight of at least one sulfur-containing amino acid selected from the group consisting of methionine, cysteine and cystine,

Tocopherol 0.5 to 1.0% by weight,

0.5 to 1.0% by weight of pyridoxine,

2.5 to 3.5% by weight of ascorbic acid,

1.0 to 2.0% by weight of calcium,

0.5 to 1.0% by weight of magnesium,

0.5 to 1.0% by weight of zinc, and

Selenium-containing dry yeast 2.5-3.5 wt%

To maintain the body's balance of minerals, to prevent in vivo absorption of heavy metals, and to exert in vitro excretion.

The slugs used in the present invention have been used for various diseases for a long time from one side and are widely used in oriental medicine or folk medicine and are now widely used in farming and are used for cateopteran and Etonian insects such as Protaetia Brevitarsis), which is dried and pulverized, can be used without any particular limitation as long as it is widely known in the art to which the present invention belongs.

It is known that oleic acid, which is known to have a blood pressure lowering action, accounts for 60% of the total fatty acids. It is known that such general ingredients of the slugs are as high as 58% of proteins and also contain a large amount of unsaturated fatty acids. In addition, phosphorus, potassium and vitamins in the minerals contain B3 and B9, which are effective to eliminate toxins and smooth blood circulation. Histidine, an essential amino acid to help alleviate allergic reactions and help blood production, And glutamic acid, which helps to improve memory, and proline, which helps to form collagen by helping to form cartilage.

Also, it is scientifically proved that the liver protects the liver from slugs, which is a herbal medicine used as a cure for liver disease in the private sector. The hepatoprotective effect of the extract of Grub was confirmed by the investigation of GOT, GPT, bile acid and bilirubin levels in carbon tetrachloride-induced acute hepatitis. In liver cirrhosis, liver fibrosis is inhibited in rats and it is expected to be effective as a therapeutic agent.

Particularly, a preferred method for preparing the dried powder of slugs is as follows: first, the slugs are slaughtered before the preparation, preferably for about 4 days, so that the waste materials remaining in the slugs are completely removed, After boiling for about 5 to 20 seconds, the slugs are killed and the germicidal effect is removed on the surface, followed by drying at 40 to 60 ° C (hot air drying or drying by a heater is possible) to prepare powders Which is produced by the method.

In case of lyophilization, it is sterilized in the process of freezing, and it is not necessary to separately heat. When it is kept at 40 to 60 ° C in the case of drying with hot air or by heating, the necessary ingredients of grub is not destroyed and roasted in dried grub It becomes a state that it is easy to eat without a sense of resistance because it becomes a taste that is like a sour taste.

In addition, it is preferable to use water from leaves of Gugija to increase the sterilization and disinfection effect rather than using general water during degreasing.

Gugija wood in the present invention is the scientific name Lycium Chinense is a deciduous broad-leaved shrub belonging to the genus Chilense. Young leaves like a green leaf are edible and also planted for ornamental purposes. It is distributed in Korea, China and Japan. The fruit is called gugija and it is used as a medicinal ingredient. It contains beta-in, beacaxanthin, carotene, thiamine, vitamin A, B1, B2 and C and it is used for chronic hepatitis, cirrhosis, It is used for lower back pain. Root skin is used as antipyretic agent such as chrysanthemum and chrysanthemum.

The content range of the functional food composition according to the present invention is determined by various experiments of the present inventors, and the content of the angular part exhibiting the remarkable effect is

600 mg of the dried slugs powder,

1500 mg of any one or more sulfur-containing amino acids selected from the group consisting of methionine, cysteine and cystine,

Tocopherol 25 mg,

Pyridoxine 17 mg,

Ascorbic acid 85 mg,

Calcium 40 mg,

Magnesium 20 mg,

10 mg of zinc, and

And 79 mg of selenium-containing dry yeast.

The at least one sulfur-containing amino acid selected from the group consisting of methionine, cysteine, and cystine may be an antioxidant enzyme such as glutathione peroxidase in the body, Removes oxygen free radicals from the body. In addition, when the free radicals increase intracellularly or heavy metal ions bind to metallothionein, they enter the cell and bind to metallothionein, or stimulate metallothionein transfection factor (MTF). As a result, the transcription proceeds and Thionein is synthesized in large quantity, and it binds again with the heavy metal to be introduced into the cell to form the metallothionein. The resulting metallochioneenes are mostly metallochioneenes, including zinc or copper, which neutralize heavy metals and their toxicity due to differences in affinity with other harmful heavy metals. In the end, it is reported that finally, the metallothionein containing harmful heavy metals is decomposed or accumulated by the lysosome in the cell or secreted by itself. Eventually, the heavy metals accumulated in the body are combined with the polymer protein to induce the production of the polymer complex metallothionein, and the amino acids and minerals necessary for production are supplied to neutralize toxic heavy metals.

Next, vitamins are contained for removal of the heavy metals of the present invention. Examples of the vitamins include tocopherol, pyridoxine and ascorbic acid. Tocopherol and ascorbic acid as major antioxidants are characterized by strongly inhibiting oxidative action in cells with glutathione, and tocopherol contains an ester compound as vitamin E. Pyridoxine plays an auxiliary role in the metabolism and synthesis of glutathione, and promotes the production of connective tissues, enzymes, nucleic acids, and immune substances, and participates in the biochemical synthesis of neurotransmitters. Pyridoxine includes vitamin B6, its hydrochloride, nitrate and sulfate.

In addition, the present invention serves as a catalyst for assisting the action of each enzyme and includes calcium, magnesium, zinc, and selenium-containing dry yeast as minerals that serve to stimulate the induction of metallothionein.

The selenium-containing dry yeast used in the present invention uses selenium-containing dry yeast containing selenium as a major component together with various nutrients as a selenium-containing dry yeast. Selenium is an essential minerals component in the human body. It works not only as an antioxidant that protects cells from free radicals produced during metabolic processes that consume oxygen, but also acts to enhance the function of the immune system.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail with reference to preferred embodiments. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to provide further explanation of the invention as claimed. Of course.

One. Example  1: Preparation of functional food composition according to the present invention

To be considered to contain 600 mg of dried powder of lime, 1500 mg of methionine, 25 mg of tocopherol, 17 mg of pyridoxine, 85 mg of ascorbic acid, 40 mg of calcium, 20 mg of magnesium, 10 mg of zinc, and 79 mg of selenium-containing dry yeast The food composition according to the present invention was prepared, the dried slug powder was poured into purified water for 10 seconds, dried by hot air at 50 ° C and then powdered to a size of about 100 mesh. The remaining components were widely used for the manufacture of health foods .

2. Comparative Example  1: Preparation of comparative food composition

A food composition was prepared in the same manner as in Example 1, except for the dried slug powder.

3. Test Example : Maintaining the body's mineral balance

In this test, two volunteers in their forties were recruited to measure changes in mineral content. Mineral levels, mineral levels, and balance ranges after ingestion of the compositions of Example 1 and Comparative Example 1 were measured in Table 1 1) and Table 2 (the result of Comparative Example 1), and the content of Ingredients 1 and Comparative Example 1 was added to the starch to make a total of 4000 mg, followed by tablets twice a day in the morning and evening This is the result after 15 days of ingestion. Mineral measurements made the inventor hair.

mineral Balance range (/ / g)  Before ingestion (/ / g) After ingestion (占 퐂 / g) Na 18 ~ 85 38.95 38.92 K 5 ~ 40 44.28 34.7 Ca 180 ~ 760 142.8 299.3 Mg 18 ~ 78 47.74 45.51 Zn 150 ~ 250 187.5 189.5 S 3000 to 55000 39718 39735 P 145 ~ 250 125.8 125.9 Cr 0.2 to 1.2 0.278 0.301 Mn 0.2 to 0.8 0.152 0.285 Co 0.01 to 0.05 0.013 0.013 Fe 6 to 15 27 12.2 Cu 15 ~ 35 65.57 32.1 Se 0.6-1.6 0.694 0.835 Li 0.01 to 0.2 0.013 0.017 V 0.02 to 0.1 0.025 0.031 Mo 0.02 to 0.1 0.036 0.035

mineral Balance range (/ / g)  Before ingestion (/ / g) After ingestion (占 퐂 / g) Na 18 ~ 85 37.75 38.25 K 5 ~ 40 49.75 48.95 Ca 180 ~ 760 289.4 299.9 Mg 18 ~ 78 47.74 45.51 Zn 150 ~ 250 151.2 158.2 S 3000 to 55000 35521 35355 P 145 ~ 250 125.8 125.9 Cr 0.2 to 1.2 0.278 0.301 Mn 0.2 to 0.8 0.141 0.142 Co 0.01 to 0.05 0.013 0.013 Fe 6 to 15 29.5 28.9 Cu 15 ~ 35 68.8 75.2 Se 0.6-1.6 0.694 0.835 Li 0.01 to 0.2 0.013 0.017 V 0.02 to 0.1 0.025 0.031 Mo 0.02 to 0.1 0.036 0.035

In Table 1, it was found that K, Ca, Mn, Fe, and Cu were out of the balance range, but after the ingestion, all the minerals were in the balance range.

However, similarly in Table 2, it was found that K, Mn, Fe, and Cu were out of balance, but they were not effective after ingestion.

4. Test Example : Evaluation of Heavy Metal Emission Efficiency by Animal Experiment

Male Sprague-Dawley rats (6-week-old, female, male, about 250 g) were purchased from a central laboratory animal and used to administer 10 cows per group to confirm that the composition of Example 1 effectively released cadmium. Lt; / RTI > The control group was administered with cadmium alone, the first group was administered with cadmium as a heavy metal, and the composition of Example 1 was administered thereto. At this time, 3 μg / g of cadmium per day and 0.1 mg / g of the example composition were orally administered together with the feed. After 4 weeks of administration, the animals were sacrificed and blood was collected to confirm the level of heavy metal discharge.

Heavy metals and extracts were administered for 4 weeks, and animals were anesthetized with zoletil (zolethyl 50, Virbac) 2 hours after the last administration. Serum was collected for the detection of lead, mercury and cadmium in the blood, , Tissues were collected to identify oxidative damage and antioxidant enzymes in the liver.

The cadmium was quantitatively evaluated using light absorbance at 228.8 nm after the atoms were excited by applying high heat to atoms using the principle of the atomic absorption spectrophotometry-graphite method. The results are shown in Table 3.

sample Cadmium blood concentration (㎍ / ℓ) Control group 6.286 Example 1 1.802

In the test results shown in Table 3, 0.033 μg / ℓ was measured in normal normal blood, but in the case of the control group administered with only cadmium, the concentration of cadmium rapidly increased and an average of 6.286 ㎍ / ℓ was confirmed.

In comparison, the average first group was measured to be 1.802 占 퐂 / liter, and it was confirmed that the concentration of cadmium in the composition according to the present invention was considerably reduced.

The composition of Comparative Example 1 was tested in the same manner, and the results are shown in Table 4. < tb > < TABLE >

sample Cadmium blood concentration (㎍ / ℓ) Control group 6.147 Comparative Example 1 6.142

The above-mentioned 4 shows that the composition of Comparative Example 1 has no cadmium emission effect.

5. Example  2: Preparation of composition according to the present invention

The composition was the same as that of the composition of Example 1, except that the water used for watering the slugs was water, not the purified water. One gram of water was added per 100 g of dried Gugija leaf and water boiled for about 1 hour was used.

6. Sensory testing

The composition of Example 1 and Example 2 were selected for a total of 50 persons, each of 20 persons in their 20s and 20s. After each composition was ingested, the subjects were asked to vote on the superior taste and to reduce their feelings. The composition of Example 2 was evaluated to be superior to that of Example 1 in that it had a savory taste and no unpleasant taste and was able to eat without irritation.

As described above, the functional food according to the present invention can be used as a functional food for the treatment and prevention of diseases caused by heavy metal poisoning by confirming the excretion of heavy metals by checking the degree of discharge of heavy metals in the blood of the present invention. It is also useful for maintaining cell activity and strengthening immunity because it maintains the normal state when the balance of the body's minerals is destroyed. It also has the advantage of being able to ingest without the feeling of using slugs.

Claims (4)

20 to 30% by weight of dried slugs,
55 to 70% by weight of at least one sulfur-containing amino acid selected from the group consisting of methionine, cysteine and cystine,
Tocopherol 0.5 to 1.0% by weight,
0.5 to 1.0% by weight of pyridoxine,
2.5 to 3.5% by weight of ascorbic acid,
1.0 to 2.0% by weight of calcium,
0.5 to 1.0% by weight of magnesium,
0.5 to 1.0% by weight of zinc, and
Selenium-containing dry yeast 2.5-3.5 wt%
A functional food composition for maintaining balance in the body, preventing in vivo absorption of heavy metals, and excretion in vitro.
The method according to claim 1,
600 mg of the dried slugs powder,
1500 mg of any one or more sulfur-containing amino acids selected from the group consisting of methionine, cysteine and cystine,
Tocopherol 25 mg,
Pyridoxine 17 mg,
Ascorbic acid 85 mg,
Calcium 40 mg,
Magnesium 20 mg,
10 mg of zinc, and
Selenium-containing dry yeast 79 mg
A functional food composition for maintaining balance in the body, preventing in vivo absorption of heavy metals, and excretion in vitro.
3. The method according to claim 1 or 2,
The dried slug powder
A method in which slugs are fasted and then lyophilized to prepare powder
Slugs are slaughtered, boiled in boiling water for 5 to 20 seconds and dried at 40 to 60 ° C to prepare a powder
A functional food composition for maintaining balance in the body, preventing in vivo absorption of heavy metals, and excretion in vitro.
The method of claim 3,
Wherein the water is used to make the leaves of the gugija when the slugs are crushed, and a functional food composition for maintaining the balance in the body, preventing the absorption of heavy metals into the living body, and excreta.