KR102114633B1 - Functional food composition with improved palatability for cadmium emission by grub and Lycium Chinense - Google Patents

Abstract

The present invention relates to a functional food composition having excellent cadmium discharge effect, which is manufactured using slugs that improve the adherence of a person taking them by improving the taste of slugs at the time of preparing dry powder of slugs, the component of which is a leaf of wolfberry Boiled slugs fastened with decoction water for 5 to 20 seconds, and then dried at 40 to 60 ° C. to form slugs dried powder, methionine, cysteine, and cystine. One or more sulfur-containing amino acids, tocopherol, pyridoxine, ascorbic acid. It is a dry yeast containing calcium, magnesium, zinc and selenium.

Description

Functional food composition with improved palatability for cadmium emission by grub and Lycium Chinense}

The present invention relates to a functional food composition with improved palatability for cadmium discharge using slugs and goji berries.

While the development of industrial society has made life easier, the environmental pollution caused by this is a threat to the human living environment. Among these, heavy metal contamination of soil or water can accumulate and accumulate in the human body through the food chain, which can cause acute or chronic poisoning. Heavy metals can easily move in vivo if they form organic or inorganic compounds depending on the type, and have various adverse effects. Crazy, and also heavy metals contained in fine dust or yellow dust can be absorbed into the human body through the respiratory system and cause more problems.

Such heavy metals are not easily discharged when they enter the body, and when their accumulation increases, they cause poisoning such as weight loss, anemia, organ biochemical and morphological changes, brain damage, and essential inorganic elements such as calcium, iron, zinc, and selenium. It acts competitively in the intestinal absorption stage, reducing these contents in the tissue. In addition, some toxic metals react with body tissues even at relatively low concentrations, slowly exhibiting poison action in the body, and especially, because of a long biological half-life, it is impossible to cure once poisoned.

For example, cadmium (Cd) is one of the most toxic substances among heavy metals and is an environmental heavy metal and is classified as a carcinogen causing cancer in the human body (Satoh, M., et al., Tohoku J. Exp. Med ., 196, pp23-32, 2002). According to epidemiological studies, even a small amount of cadmium, when ingested for a long time, accumulates in the target organs, resulting in adverse health effects.

The total amount of cadmium consumed by humans for a lifetime is approximately 2000 mg (Klaassen, CD, Casarett and Doull's Toxociology, McGraw-Hill Medical Publishing Division, New York, pp822-826, 2001), and cadmium present in food or studio air When chronically consumed, cadmium-accumulated tissue cells can kill cells and cause organ abnormalities (Falck, FYJr., Et al., Am. J. Ind. Med., 4, pp541 -549, 1983). Cadmium mainly accumulates in the liver and kidneys, and even at low doses, chronically accumulated cadmium causes lung, liver and renal tubular diseases.

Morphological modifications of cells induced by cadmium exposure include cell degeneration, inflammatory responses, and fibrosis. The pathological mechanism of tissue damage induced by cadmium is associated with cell necrosis and high cell replacement rates (Sauer, J.M., et al., Toxicology, 121, pp155-164, 1997).

In general, low doses of toxic substances tend to cause apoptosis, but high levels of toxic exposure later cause cell necrosis (Raffray, M., et al., Pharmacol. Ther., 75, 153 -177, 1997). Decomposition of proteins by apoptosis stimulates the release of the protein-bound bad, promotes the potential of the bed to mitochondria, and increases the cell death by interacting with BclXL in the mitochondria (Nomura, M., et al., J. Biol. Chem., 278, pp2058-2065, 2003).

Against this background, the efficacy of fibrin, protein, selenium and trace elements has been studied as part of research to alleviate the symptoms of lead and cadmium poisoning, and black tea and green tea catechin improve renal dysfunction caused by cadmium poisoning and normalize blood pressure. Research has been reported.

In addition, when a harmful substance enters the body, biotransformation proceeds to quickly excrete the harmful substance from the body, and through this action, the harmful substance is excreted outside the body. This metabolic process is largely described in two stages, that is, foreign substances such as environmental pollutants or drugs absorbed into the body are excreted in the liver tissue by a phase 1 reaction or a phase 2 reaction, or excreted through both phases. The phase1 enzyme contains cytochrom P450 and is converted to a highly reactive compound by changing to have reactive electrons through oxidation, reduction, and hydrolysis reactions of hydrophobic compounds. Phase 2 enzymes include glutathion S-transferase, UDP-glucuronosyl transferase, and guinone reductase, which help to convert highly metabolizable substances into stable substances and facilitate excretion (Liska DJ. Alter med rev 3: 187 -198,1998).

Recently, many studies have been conducted on the correlation between gulcosinolates, which are sulfur-containing substances of cruciferous vegetables, such as cauliflower, cabbage, kale, and broccoli, as cancers, and cancer as a potential cancer prevention substance. It has been reported to have action.

As such, studies on anticancer activity related to the induction of phase 1 reaction or phase 2 enzymatic activity are active, but on the other hand, studies on detoxification of heavy metals and scientific data are insufficient, and heavy metals can induce accumulation and discharge in the body. The research on functional foods is still weak.

In addition, maintaining the mineral balance in the body is very important for the maintenance of immune function, maintenance of cell activity, and disease prevention. Research on foods that correct the destroyed balance and maintain the balance at the same time is also weak.

[Document 1] Korean Patent Publication No. 2000-0004499 [Document 2] Korean Patent Publication No. 2003-0079205 [Document 3] Korean Patent Publication No. 2004-0107853 [Document 4] Korean Patent Publication No. 2004-0107852 [Document 5] Korean Patent Publication No. 2005-0036093

Accordingly, the present invention is to be used as a health food having a significant effect on cadmium discharge when using slugs while conducting research on a composition capable of maintaining the balance of minerals in the body and alleviating damage to harmful heavy metals, lead and cadmium toxicity It has been confirmed that the present invention has been accomplished by improving the taste of dried powdery slugs and completing a method of removing the discomfort from slugs.

Therefore, it is to provide a functional food composition with improved palatability for cadmium discharge using slugs and goji berries, which improves the taste of slugs when improving the taste of slugs when preparing dry powders of slugs.

In order to achieve the above technical problem, the present invention

600 mg of slug dry powder prepared from powder by boiling the leaves of wolfberry tree with decoction water for 5 to 20 seconds and then drying at 40 to 60 ° C.

1500 mg of any one or more sulfur-containing amino acids selected from the group consisting of methionine, cysteine and cystine,

Tocopherol 25 mg,

Pyridoxine 17 mg,

85 mg ascorbic acid,

40 mg calcium,

Magnesium 20 mg,

Zinc 10 mg, and

Selenium-containing dry yeast 79 mg

It provides a functional food composition for maintaining the balance of minerals in the body, preventing the absorption of heavy metals in vivo and discharging the body, characterized in that it comprises.

The functional food of the present invention can be used as a functional food having an excellent cadmium discharge effect, and further improving the patient's compliance by improving the unpleasant taste of the slug, thereby further increasing the usability.

Hereinafter, the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice it.

Functional food composition according to the invention

600 mg of slug dry powder prepared from powder by boiling the leaves of wolfberry tree with decoction water for 5 to 20 seconds and then drying at 40 to 60 ° C.

1500 mg of any one or more sulfur-containing amino acids selected from the group consisting of methionine, cysteine and cystine,

Tocopherol 25 mg,

Pyridoxine 17 mg,

85 mg ascorbic acid,

40 mg calcium,

Magnesium 20 mg,

Zinc 10 mg, and

79 mg dry yeast containing selenium

It is characterized by maintaining the balance of minerals in the body, preventing absorption of heavy metals in vivo, and excreted outside the body.

The slug used in the present invention has been used as a medicine for various diseases for a long time from oriental medicine and is currently widely used in farming and is used for herbal medicine or folk remedies, and white beetle (Protaetia) which is an insect of Coleopteran and Etonian. brevitarsis), a method for drying and pulverizing it into larvae can be used without particular limitation as long as it is well known in the art.

The general component of these slugs is about 58% very high in protein, and also contains a large amount of unsaturated fatty acids. Especially, it is known that oleic acid, which is known to lower blood pressure, accounts for 60% of all fatty acids. In addition, phosphorus, potassium, and vitamins in the minerals include B3 and B9, which have the effect of eliminating toxins and smoothing blood circulation, alleviating serious injuries and helping blood production. Histidine, an essential amino acid in childhood, improves cognitive ability And it contains a large amount of proline (proline) that helps to form cartilage by helping to form glutamic acid and collagen, which help improve memory.

In addition, slug is a herbal medicine favored as a remedy for liver disease in the private sector. The liver protective effect of the slug extract was examined for the levels of GOT, GPT, bile acids, and bilirubin in acute hepatitis induced by carbon tetrachloride. The liver cirrhosis inhibits liver fibrosis in mice in progress, and is expected to be effective as a therapeutic agent.

In particular, a preferred method for preparing dried slugs is to fasten slugs before preparation, preferably fasting for about 4 days to completely remove the residue remaining in the slug's intestines, then boil water with the leaves of wolfberry trees to boil about 5 to 5 It is produced by a method of manufacturing a powder by killing slugs for 20 seconds, removing the sterilizing effect and stains on the surface, and then drying at 40 to 60 ° C (both hot air drying or drying by a heater).

The wolfberry tree in the present invention has the scientific name Lycium As a chinense , it is a deciduous broad-leaved shrub belonging to the family Asteraceae. Young leaves that look like red pepper are edible and planted for ornamental purposes, and are distributed in Korea, China, and Japan. The fruit is called wolfberry, and is used for medicinal purposes. Its ingredients include betaine, beaxanthin, carotene, thiamine, vitamin A, B1, B2, C, etc., chronic hepatitis, cirrhosis, tonic, antipyretic It is used for low back pain, and the root bark is called golgolpi (地骨皮) and is used as an antipyretic agent for sogal and chills in oriental medicine.

Any one or more sulfur-containing amino acids selected from the group consisting of methionine, cysteine, and cystine produce antioxidant enzymes, such as glutathione peroxidase, in the body, resulting from heavy metals. Free radicals containing oxygen are removed from the body. In addition, when a large amount of heavy metal ions to bind to the metallothionein increase or free radicals in the cell is introduced into the cell, it is removed by binding to the metallothionein or stimulates the metallothionein transciprtion factor (MTF). As a result, transcription proceeds, and a large amount of thionein is synthesized, and is recombined with the heavy metal to be introduced into the cell to form a metallothionein. The resulting metallothioneins are mostly metallothioneins containing zinc or copper, which neutralize heavy metals and their toxicity due to differences in affinity with other harmful heavy metals. Eventually, there are reports that metallothionein containing harmful heavy metals is finally decomposed or accumulated by Lysosome in cells or secreted by itself. Eventually, the heavy metals accumulated in the body are combined with a polymer protein to induce the production of metallothionein, a polymer complex, and neutralize the toxicity of harmful heavy metals by supplying amino acids and minerals necessary for production.

Next, a vitamin is included for removing heavy metals of the present invention, and the vitamins include tocopherol, pyridoxine and ascorbic acid. Tocopherol and ascorbic acid are major antioxidants and are characterized by strongly inhibiting oxidative action in cells together with glutathione, and tocopherol contains an ester compound as vitamin E. Pyridoxine plays an auxiliary role in the metabolism and synthesis of glutathione, promotes the production of connective tissue, enzymes, nucleic acids, and immune substances, and is involved in biochemical synthesis of brain neurotransmitter. Pyridoxine is vitamin B6 and includes its hydrochloride, nitrate and sulfate salts.

In addition, the present invention serves as a catalyst to assist the action of each enzyme, and is contained as a mineral that serves to stimulate the induction of metallothionein, and includes calcium, magnesium, zinc and selenium-containing dry yeast.

The selenium-containing dry yeast used in the present invention uses dry yeast containing selenium as a main component along with various nutrients as selenium-containing dry yeast. Selenium is a mineral component that is essential for the human body and acts as an antioxidant that protects cells from free radicals generated in the process of metabolism that consumes oxygen, as well as strengthens the function of the immune system.

Next, the present invention will be described in detail through preferred embodiments. Specific examples of the embodiments are intended to illustrate the present invention, but are not limited thereto, and can be implemented in various ways within the scope of the claims and detailed description of the invention, and also belong to the scope of the present invention Of course it is.

One. Example  1: Preparation of functional food composition according to the present invention

Powdered dry powder 600 mg, methionine 1500 mg, tocopherol 25 mg, pyridoxine 17 mg, ascorbic acid 85 mg, calcium 40 mg, magnesium 20 mg, zinc 10 mg, and selenium-containing dry yeast 79 mg A food composition according to the invention was prepared, and the slug dried powder was boiled in purified water for 10 seconds, then dried with a hot air at 50 ° C. and then used as a powder of about 100 mesh, and the remaining components were widely used in health food manufacturing. It was purchased and manufactured.

2. Comparative example  1: Preparation of comparative food compositions

The food composition was prepared in the same manner as in Example 1 except for the slug dry powder.

3. Test Example: Evaluation of heavy metal emission efficiency through animal experiments

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Male Sprague-Dawley rats (6 weeks old, males, about 250 g) were purchased from central laboratory animals and used in this experiment to determine whether the composition of Example 1 effectively discharges cadmium. Did. The control group was administered only with cadmium, the first group was administered with the composition of Example 1 while administering cadmium as a heavy metal. At this time, cadmium was 3 μg / g per day, and the composition of the example was orally administered with 0.1 mg / g of feed. After 4 weeks of administration, animals were sacrificed to collect blood to confirm the degree of heavy metal discharge.

After administration of heavy metals and extracts for 4 weeks, 2 hours after the last administration, animals were overesthetized with zoletil (zoletil 50, Virbac), and then serum was collected for detection of lead, mercury, and cadmium in the blood. , In order to confirm the oxidative damage in the liver and the distribution of antioxidant enzymes in tissues, tissues were collected.

Cadmium was subjected to the atomic absorption spectroscopy-graphite method inspection principle to make the atoms excited by applying high heat, and then quantitatively evaluated using cadmium atoms absorbed at 228.8 nm, and the results are shown in Table 1.

sample Cadmium blood concentration (㎍ / ℓ) Control 6.286 Example 1 1.802

Looking at the test results in Table 1, 0.033 μg / ℓ was measured in normal normal blood, but in the case of a control group administered with cadmium only, the cadmium concentration increased rapidly, and an average of 6.286 μg / ℓ could be confirmed.

Compared to this, the average first group was measured as 1.802 µg / L, and it was confirmed that the concentration of cadmium was significantly reduced in the case of the composition according to the present invention.

The test was conducted on the composition of Comparative Example 1 in the same manner, and the results are shown in Table 2.

sample Cadmium blood concentration (㎍ / ℓ) Control 6.147 Comparative Example 1 6.142

Looking at the results of Table 2, it was confirmed that the composition of Comparative Example 1 had no cadmium discharge effect.

4. Example 2: Preparation of the composition according to the invention

It was prepared in the same manner as in the composition of Example 1, but there is a difference in using water of decoction of goji berry instead of purified water as water for boiling slugs. 1 l of water was added per 100 g of dried goji berry leaves, and water boiled for about 1 hour was used.

5. Sensory test

With respect to the compositions of Examples 1 and 2, a total of 50 persons were selected for each of 20 men and women in their 20s, and after ingesting each composition, votes were made on the side with excellent taste and the feeling was reduced. In the composition of Example 2, it was evaluated to be superior to Example 1 because it had a savory taste and had no other unpleasant taste, so that it could be eaten without rejection.

As described above, the composition according to the present invention is used as a functional food for new treatment and prevention of diseases caused by heavy metal poisoning by confirming that the functional food of the present invention is excellent in excretion of heavy metals as a result of checking the discharge level of heavy metals in the blood. In addition, when the balance of minerals in the body is destroyed, it is restored to normal, and in the normal state, it is maintained, which is very useful for maintaining cell activity and strengthening immunity. It also has the advantage of being able to consume slugs without a feeling of rejection.

Claims (1)

Boiled leaves of wolfberry berry fastened with water, boiled for 5 to 20 seconds, and dried at 40 to 60 ° C to improve the taste of powdery dried powder, which is improved to 600 mg,
1500 mg of any one or more sulfur-containing amino acids selected from the group consisting of methionine, cysteine and cystine,
Tocopherol 25 mg,
Pyridoxine 17 mg,
85 mg ascorbic acid,
40 mg calcium,
Magnesium 20 mg,
Zinc 10 mg, and
Selenium-containing dry yeast 79 mg
Functional food composition with improved palatability for cadmium discharge, characterized in that it comprises.