KR20030079205A - Dendropanax morbifera Lev. extract having a protective effect of hepatic cells, Dendropanax morbifera Lev. fractions, and pharmaceutical composition containing the same - Google Patents

Abstract

PURPOSE: Provided are an extract and fractions of Dendropanax morbifera H Lev having liver protection effect and a pharmaceutical composition containing them, thereby preventing and treating liver diseases, such as fatty liver, hepatitis, hepatocirrhosis and the like. CONSTITUTION: An extract of Dendropanax morbifera H Lev is obtained by extracting Dendropanax morbifera H Lev resin with an alcohol solvent at 50-100 deg.C for 5-24 hours, followed by cooling, filtration and concentration. The extract is fractioned with solvents, particularly, hexane, chloroform, butanol, and water sequentially.

Description

Hwangchil extract with hepatoprotective effect, Hwangchil fraction and pharmaceutical composition containing them {Dendropanax morbifera Lev. extract having a protective effect of hepatic cells, Dendropanax morbifera Lev. fractions, and pharmaceutical composition containing the same}

The present invention relates to a Hwangchil extract having a hepatocellular protective effect, and more particularly, to a Hwangchil extract, a fraction thereof, and a pharmaceutical composition and functional food containing the same, which can prevent and treat liver diseases such as fatty liver, hepatitis, and cirrhosis. .

Chronic liver disease, including liver cancer, is one of the highest prevalence rates among hospitalizations among adults in Korea (Statistics Annual Report, 1994). According to the 1996 Statistical Yearbook of Causes of Death, the number of liver cancer mortality in men per 100,000 population is 33, the highest in the world, and the mortality rate from chronic liver disease is 44, per 100,000 population.

Acute viral hepatitis is a disease that is an important cause of chronic liver disease such as cirrhosis of the liver and liver cancer, and is a systemic infection mainly affecting the liver and is known to be caused by hepatitis viruses such as A, B, C, D and E. In addition to viral hepatitis, one of the main causes of liver disease is alcohol. Hepatic diseases caused by alcohol are classified as alcoholic fatty liver, alcoholic hepatitis and alcoholic cirrhosis, and appear in various forms, ranging from accumulation of some fat in the liver to advanced cirrhosis. Fatty liver is the earliest common alcoholic liver disease caused by excessive drinking. Alcoholic hepatitis is reported in 20-30% of excessive chronic alcohol intake, and alcoholic cirrhosis is reported in 10-15% of chronic alcohol intake.

Dendropanax morbifera Lev. Belonging to the family Elmaceae is an evergreen broad-leaved arboreous tree growing in the southern coastal region of Jeju Island and Jeju Island. ) Is called. Hwangchil has been used as a rare paint that emits the golden color of emperor's armor, helmets, and other metal ornaments since the Three Kingdoms. It is a Korean envoy, written in the Goryeo Dynasty, Guilin History, Guilin, and Haedong History. It is recorded and remains a specialty of Baekje, the old book of Tang Dynasty History Book. It is recorded in the herbaceous tree of Lee Si-jin that Hwangchil-tree is effective and effective for removing heat, treating eye and burns, and leprosy.

The components of the yellow lacquer are composed of 66.7% of non-volatile components, 10.8% of aromatic components, 8.1% of moisture, and 14.4% of solid components, which are the coating components forming the golden coating. The aromatic components consist mainly of β-cubebene, γ-selinene, and δ-cadinene of sesquiterpenes, and these components are known to exhibit sedative and tonic effects on the nervous system. .

The pharmacological action of the hwangchil discovered so far, only the anticancer action in the leaf extract fraction, and the antioxidant activity in the hwangchil extract fraction was found to be slightly weaker than α-tocopherol (Korean Patent Publication No. 2000-0004499).

The present inventors earnestly conducted a study on the yellow lacquer, and as a result, the extract obtained from the yellow lacquer was confirmed for the first time that hepatocellular protective effect was completed the present invention.

Accordingly, an object of the present invention is to provide a hwangchil extract and its fractions and liver cell protective composition comprising them having the effect of preventing and treating liver diseases such as fatty liver, hepatitis, cirrhosis and the like.

1 is a diagram schematically illustrating a method for obtaining an alcohol extract and fractions thereof of the yellow larch ( Dendropanax morbifera Lev.) Of the present invention.

The present invention provides a hwangchil extract having a hepatocyte protective effect extracted from the resin of the Hwangchil wood ( Dendropanax morbifera Lev.) With an alcohol solvent.

The present invention is also a hepatocellular protective effect, characterized in that the concentrated liquid obtained by evaporating and condensing the supernatant obtained by cooling the extract obtained by hot water at 50-100 ° C. and hot water at 50-100 ° C. for 5 to 24 hours. It provides a yellow lacquer extract having.

The present invention also provides a hwangchil fraction having a hepatocyte protection effect, which is selected from the group consisting of hexane fraction, chloroform fraction, butanol fraction and water fraction obtained by sequentially repeatedly extracting the yellow lacquer extract hexane, chloroform, butanol and water as a solvent. to provide.

The present invention also provides a composition having a hepatocyte protective effect comprising the yellow lacquer extract and / or the yellow lacquer fraction as an active ingredient.

The present invention also provides a pharmaceutical composition having a hepatocellular protective effect, which comprises a yellow lacquer extract and / or a fraction thereof and a pharmaceutically acceptable carrier.

The present invention also provides a food composition having a hepatocyte protective effect comprising a yellow lacquer extract and / or a fraction thereof.

Hereinafter, the present invention will be described in detail.

The first aspect of the present invention is to provide a hwangchil extract having a hepatocyte protective effect extracted from the resin of Hwangchil wood ( Dendropanax morbifera Lev.) With an alcohol solvent.

An example of the extraction method of the yellow lacquer of the present invention is as follows.

First, the yellow lacquer is added to an extraction solvent such as methanol, ethanol, isopropanol, butanol and the like, and heated at 50 to 120 ° C. for 5 to 24 hours, sonicated at room temperature, or cooled at room temperature or 4 ° C. for 5 to 7 days. After extraction, the extract is filtered to obtain a supernatant. All the supernatants obtained by repeating the above extraction and filtering operations one or more times are combined and concentrated by evaporation of the solvent. The extraction and filtration operations are preferably repeated several times, and the concentrate may be dried to obtain a powder.

The second aspect of the present invention is also selected from the group consisting of hexane fraction, chloroform fraction, butanol fraction and water fraction obtained by sequentially repeatedly extracting the yellow lacquer extract using hexane, chloroform, butanol and water as a solvent, hepatocellular protective effect To provide a yellowish fraction having.

In this way, in order to obtain a fraction according to the polarity, the powdery methanol extract may be repeatedly extracted and filtered with organic solvents hexane, chloroform, butanol and water in order to be repeated several times to obtain a yellow lacquer fraction. The obtained fractions may be dried and used in liquid or powder form.

A method of obtaining an alcohol extract and fractions thereof of the yellow larch ( Dendropanax morbifera Lev.) Of the present invention is shown schematically in FIG.

The hwangchil extract of the present invention and its fractions, as in the following experimental example, has an excellent liver cell protective effect. Therefore, it is suitable for preventing or treating liver diseases such as fatty liver, hepatitis and cirrhosis.

A third aspect of the present invention is to provide a pharmaceutical composition having a hepatocellular protective effect, characterized in that it comprises a yellow lacquer extract and / or fractions and a pharmaceutically acceptable carrier.

The pharmaceutical composition for protecting hepatocytes according to the present invention may be composed only of the above-described yellow lacquer extract or a fraction thereof, and may further include other drugs and conventional pharmaceutical additives. In addition, the present invention is formulated into a pharmaceutical preparation in solid, semi-solid or liquid form suitable for oral or parenteral administration by combining the pharmaceutically acceptable carrier and the hwangchil extract of the present invention or a fraction thereof upon clinical administration. Pharmaceutical compositions can be prepared and administered.

Suitable formulations of the pharmaceutical composition include, but are not limited to, tablets, dragees, hard or soft capsules, solutions, suspensions or emulsions, injections, suppositories, and the like. Pharmaceutically acceptable carriers which may be suitably used for the above purposes may be solid or liquid, any one of which may act as a diluent, flavor, solubilizer, lubricant, suspending agent, binder, disintegrant, or the like. It may include more than that.

The hwangchil extract of the present invention, fractions thereof or liver cell protective agent composition comprising the same may be administered 0.01 ~ 0.10 g of the hwangchil extract or fractions thereof as dry powder per kg of body weight per day. The dosage can vary depending on the needs of the patient, the condition to be treated, and the compound to be used, and can be administered several times at regular time intervals as needed.

The use of the pharmaceutical composition of the present invention is not necessarily limited to a medicament, and can also be used as a dietary supplement such as the usage of squalane, aloe and the like.

As used herein, the term "health supplement" is a composition used without a drug license, it means a capsule and the like used in the usage, such as squalene and the like.

A fourth aspect of the present invention is to provide a food composition having a hepatocellular protective effect comprising a yellow lacquer extract and / or a fraction.

The food composition is preferably a beverage, medicine, kimchi, yogurt, milk, ice cream, bread, rice cake or noodles, but is not limited thereto.

Hereinafter, preferred embodiments of the present invention will be described in detail, and the present invention may use various changes, modifications, and equivalents. It is clear that the present invention can be applied in the same manner by appropriately modifying the following examples. Therefore, the present invention is not limited to the description of the following examples. However,% in this specification and an Example means the volume%.

Example 1: Preparation of the yellow lacquer extract and the yellow lacquer fraction

The yellow lacquer used in this example was a sample taken directly from June to July at Jindo. 100 g of the extracted yellow lacquer was put in 500 ml of 80% methanol (methanol: water = 4: 1), and equipped with a reflux condenser, followed by warming in a 95-100 ° C. water bath for 12 hours. The extract was cooled to about 50 ° C. and filtered through several layers of gauze to obtain a supernatant. This extraction and filtration was repeated three times to combine the supernatant and concentrated by completely evaporating methanol under reduced pressure using a rotary evaporator (rotary evaportor) to prepare a hwangchil methanol extract of the present invention (78g).

In addition, the hwangchil methanol extract is added appropriate amount of hexane, chloroform, butanol and water, and sequentially fractionated into the soluble part for these solvents, each fraction is concentrated under reduced pressure to the present invention, the hwangchil hexane fraction (10g), chloroform fraction (30g), Butanol fraction (3 g) and water fraction (5 g) were prepared.

Example 2

Hwangchil extract concentrate and fractions finally obtained in Example 1 was frozen at -80 ℃ and then lyophilized to obtain a powder.

Example 3

100 g of yellow lacquer was added to 500 ml of 80% methanol (methanol: water = 4: 1), and then cooled at room temperature for 1 day or 7 days at 4 ° C., and then filtered through a layer of gauze to obtain a supernatant. The supernatant obtained by repeating this extraction and filtration three times was combined and concentrated by evaporating methanol completely under reduced pressure using a rotary evaporator, and then dissolving it in a small amount of distilled water. .

Example 4

The yellow lacquer extract concentrate obtained in Example 3 was frozen at −80 ° C. and lyophilized to obtain a powder.

Example 5

The dried powder of the Hwangchil extract obtained in Example 4 was added to a separatory filter, and hexane, chloroform, ethyl acetate, butanol and water as appropriate solvents were added in an appropriate amount. The extract was sequentially extracted at room temperature for 1 day and then filtered through a filter paper of several layers. Drunk. The supernatant obtained by repeating this extraction and filtration three times was combined and concentrated by completely evaporating the solvent under reduced pressure using a rotary evaporator.

Example 6

The hexane, chloroform, ethyl acetate, butanol or water fractions finally obtained in Example 5 were frozen at -80 ° C and lyophilized to obtain a powder.

Example 7: Preparation of Tablets

Hwangchil extract prepared according to Example 1 or a fraction thereof was sieved, mixed with lactose, starch and pregelatinized corn starch, and then purified water was added appropriately and granulated into powder. The granules were dried and then mixed with magnesium stearate and compressed to obtain tablets. The components used in the purification and the amount thereof used are as follows.

Hwangchil extract of Example 1 or a fraction thereof 5.0 mg

Lactose BP 150.0 mg

Starch BP 30.0 mg

Pregelatinized Corn Starch BP 15.0 mg

1.0 mg magnesium stearate

Example 8 Preparation of Capsules

Hwangchil extract of Example 2 or a fraction thereof was sieved and mixed with excipients, and then filled into gelatin capsules to prepare capsules. The ingredients used and their amounts used are as follows.

5.0 mg of Hwangchil extract or fractions thereof

Starch 1500 100.0 mg

Magnesium Stearate BP 1.0 mg

Example 9 Preparation of Syrup

Dissolve the white sugar in 500 ml of purified water, and separately dissolve the carboxymethylcellulose sodium in 400 ml of purified water in a separate container, mix it with the solution in which the white sugar was dissolved, add methylparaben and propylparaben to dissolve, add ethanol, and then purified water. The volume of the total solution was set to 1000 ml. Hwangchil extract of Example 2 or a fraction thereof sieved thereto was suspended to obtain a syrup. The ingredients used and their amounts used are as follows.

5.0 g of yellow lacquer extract of Example 2 or a fraction thereof

637.5 g per bag

2.0 g of sodium carboxymethylcellulose

0.28 g of methyl paraben

0.12 g of propylparaben

20 ml of ethanol

Example 10 Preparation of Functional Beverages

A general functional beverage base comprising 0.01% by weight of the hwangchil extract or fractions thereof, 0.05% by weight of food coloring, 0.05% by weight of orange essence, 7.0% by weight of fructose, 0.1% by weight of citric acid, and 0.05% by weight of vitamin C One composition was prepared and then purified water was added to prepare a beverage.

Example 11 Preparation of Medicine

The deodorized purified 40% by weight alcohol was diluted with distilled water, 0.01% by weight of the yellow lacquer extract of the present invention prepared according to Example 1 was added, and stevioside, high fructose, amino acids, citric acid, salt and the like were added to the alcohol-containing concentration. Prepared from 15 to 30% by weight.

Example 12 Preparation of Dietary Supplements

Similar to the method of Example 8, the capsule was prepared by mixing and filling the yellow lacquer extract of Example 1 or its fraction (5.0 mg) with starch 1500 (100.0 mg) and magnesium stearate BP (0.5 mg) in a gelatin capsule. It was. This capsule can be used as a dietary supplement without requiring drug approval.

Experimental Example 1: Determination of ethanol-induced hepatotoxicity inhibitory effect by the administration of Hwangchil extract and Hwangchil fraction of the present invention

In this experimental example, hepatoprotective effects on the co-administration of ethanol extract and fractions and ethanol were tested.

(1) Measurement of the concentration of GOT, GPT and ALP in the blood

Since the liver has a high concentration of enzymes and a hematogenous structure that is easy to leak into the blood, measuring the enzyme activity of the liver released from the damaged liver is one of the most useful methods for studying liver damage. Necrosis of liver cells and destruction of liver tissue due to hepatic damage are released into the blood and show high activity (Plaa et al., 1982). Therefore, liver function can be measured by measuring GOT and GPT in blood. In addition, ALP is an enzyme released into the blood at the time of tissue damage, and the level of hepatic cell damage can be known by measuring ALP in the blood. The specific measuring method was as follows.

Sparague-Dawley male rats (10 rats per test group), weighing 150-250 g, were kept at 22 ± 2 ° C for at least 2 weeks to adapt to the laboratory environment and used for experiments. Solid feed (Samyang feed) and water It was supplied sufficiently. Hwangchil methanol extract of Example 1 and each of the fractions (hwangchil hexane fraction, chloroform fraction, butanol fraction and water fraction) was suspended in distilled water and administered once orally at 100mg / kg. Ethanol was intraperitoneally administered and fasted at 6 g / kg immediately after administration of the hwangchil extract and fractions to cause liver damage. After 24 hours of ethanol administration, anesthesia was anesthetized, the abdominal midline was excised to collect blood from the heart, and the plasma obtained by centrifugation at 3,000 rpm for 20 minutes was used as a sample. Plasma glutamate oxalate transaminase (GOT), glutamate pyruvate transaminase (GPT) and alkaline phosphatase (ALP) in the plasma The analysis was carried out.

1.0 ml of GOT substrate solution was heated in a 37 ° C. water bath for 2 to 3 minutes, 0.2 ml of test serum was added and warmed in a 37 ° C. water bath for 60 minutes, 1.0 ml of a coloring solution was added, and left at room temperature for 20 minutes. After adding 10.0 ml of NaOH and mixing well, the absorbance was read by UV / VIS spectrophotometer (Jasco V-530) at 505 nm with distilled water as a control and the concentration of blood GOT was measured.

In addition, 1.0 ml of GPT substrate solution was heated in a 37 ° C. water bath for 2-3 minutes, 0.2 ml of test serum was added, warmed in a 37 ° C. water bath for 30 minutes, 1.0 ml of a coloring solution was added, and left at room temperature for 20 minutes. After 10.0 ml of N-NaOH was added and mixed well, the absorbance was read at 505 nm with distilled water as a control to measure the concentration of blood GPT.

In addition, 2.0 ml of ALP substrate solution was heated in a 37 ° C. water bath for 2-3 minutes, 0.05 ml of the test serum was added, followed by warming for 15 minutes in a 37 ° C. water bath, and 2.0 ml of a coloring solution was added and left for at least 10 minutes. Using the blank solution operated in the same manner as described above, the absorbance was read at 570 nm to measure the concentration of blood ALP.

All experimental results calculated the mean and standard error, and the differences between the groups were statistically significant when the p-value was less than 0.05 using Student's t-test. The measurement results are shown in Table 1 below.

process Number GOT (IU / L) GPT (IU / L) ALP (IU / L) normal 10 60.7 ± 3.2 26.4 ± 6.4 62.7 ± 31.8 Ethanol (Control) 10 105.0 ± 1.6 47.5 ± 5.7 177.3 ± 27.3 Hwangchil Methanol Total Extract + Ethanol 10 87.3 ± 8.3 32.3 ± 4.3 ^* 152.21 ± 21.3 Hwangchil Water Fraction + Ethanol 10 92.8 ± 10.4 36.8 ± 3.9 ^* 143.25 ± 23.9 Hwangchil Butanol Fraction + Ethanol 10 85.0 ± 13.1 ^* 35.5 ± 6.7 ^* 136 ± 15.3 ^* Hwangchil Chloroform Fraction + Ethanol 10 88.8 ± 9.6 ^* 32.3 ± 9.4 ^* 157 ± 29.0 Hwangchil Hexane Fraction + Ethanol 10 73.7 ± 6.2 ^** 27.3 ± 6.6 ^** 148 ± 22.1

(*: p <0.05, **: p <0.01)

As can be seen from Table 1, when the total extract and the fractions of the hwangchil methanol and its fractions were administered simultaneously with ethanol, the GOT showed a significant decrease in both the total extract and the group of Hwangchil butanol extract and its fraction administration group, even in the case of GPT All of the fraction administration groups showed a significant decrease. In particular, for the administration of the lactose hexane fraction. Both GOT and GPT were reduced to near normal values, indicating that ethanol is highly effective in protecting liver damage.

In addition, ALP also significantly decreased in both the group and the fraction administration group. Thus, it can be seen that the hwangchil fraction and its fractions protect the hepatocytes from liver tissue damage by ethanol administration.

(2) Measurement of the concentration of malondialdehyde (MDA) in hepatocytes

The phospholipids of the cell membrane are formed through lipid peroxy radicals and lipid endoperoxide radicals and lipid endoperoxides by the attack of free radicals, which are then decomposed to form MDA. Lipid peroxidation is widely used as an indicator of the degree of oxidative stress (Johansson, .I et al., 1985; Ayene, IS et al., 1992). In vivo, lipid peroxide acts on proteins, RNA, and DNA, which are components of cells, resulting in dysfunction and biochemical conclusions such as mitochondria, which has attracted attention as a substance that promotes disease and aging. In particular, since lipid peroxide is well formed in the biological membrane and polyunsaturated fatty acid, which is a major component of the biological membrane, is changed in fat composition by lipid peroxide formed of free radicals, the function of the biological membrane is degraded and fluidity is reduced to reduce fluidity. And reduced permeability, which impedes maintenance of homeostasis (Plaa, GL, 1976; Curtis, MT, 1984; Lacko, AG, 1984).

Ethanol generates oxygen radicals in the process of metabolic intermediates by CYP 2E1, a hepatic metabolic enzyme, resulting in lipid peroxidation of hepatocytes, structural changes in biological membranes, and destruction of the enzyme system inside, resulting in lipid peroxidation in blood and tissues. Since the content is increased, the higher the MDA concentration of hepatocytes means that the liver function is lowered, MDA concentration may be an indicator of liver function test.

The specific method for measuring the MDA inhibitory effect by administration of the hwangchil extract and fractions thereof of the present invention was as follows.

Hwangchil methanol extract and its fractions, and ethanol were administered according to the method described in (1) above, and the liver tissues extracted 24 hours after the final ethanol administration were washed with physiological saline, severed and quantitated, and weighed by 5 times. The corresponding phosphate buffer solution was added and homogenized. 0.5 ml of homogenized sample and 0.4 ml of SDS were added and solubilized in a 37 ° C. water bath. 2 ml of 0.1N HCl and 0.67% TBA solution were added and mixed, followed by heating for 50 minutes on a 95 ° C. water bath. The reaction was stopped on an ice water bath and 5 ml of butanol was added. After centrifugation at 3,000 rpm for 10 minutes, the absorbance at 535 nm was measured. Blanks were obtained by the same reaction procedure, containing all reagents except lipids. The measurement results are shown in Table 2 below.

process Number MDA normal 10 0.0154 ± 0.002 Ethanol (Control) 10 0.0358 ± 0.001 Hwangchil Methanol Total Extract + Ethanol 10 0.0302 ± 0.003 ^* Crude Water Fraction + Ethanol 10 0.0277 ± 0.002 ^** Hwangchil Butanol Fraction + Ethanol 10 0.0270 ± 0.004 ^* Hwangchil Chloroform Fraction + Ethanol 10 0.0290 ± 0.003 ^* Hwangchil Hexane Fraction + Ethanol 10 0.0325 ± 0.002

(*: p <0.05, **: p <0.01)

As shown in Table 2, when the Hwangchil extract of the present invention and its fractions were administered and the concentration of MDA in hepatocytes was measured, a significant decrease in MDA was observed. In particular, it was found that hwangchil butanol and chloroform fractions inhibited the formation of lipid peroxide by more than 50%, showing hepatoprotective action, and this action is related to CYP 2E1 enzyme function.

Experimental Example 2. Determination of ethanol-induced hepatotoxicity inhibitory effect on combating the yellow and yellow extract of the present invention

In this experimental example, the hepatoprotective effect of the hwangchil methanol extract and the fraction of the filtrate was tested.

Sparague-Dawley male rats weighing 150-250 g were reared at 22 ± 2 ° C. for at least 2 weeks to adapt to the laboratory environment, and were used in the experiment. Solid feed (Samyang feed) and water were sufficiently supplied. Hwangchil methanol extract of Example 1 and each of the fractions (hwangchil hexane fraction, chloroform fraction, butanol fraction and water fraction) was suspended in distilled water and administered once orally at 100mg / kg. Ethanol was intraperitoneally administered and fasted 24 hours after hwangchil extract and fractions to induce liver damage. Isolation of blood and blood serum, biochemical analysis of GOT, GPT and ALP of blood, and biochemical analysis of MDA of hepatocytes were performed in the same manner as in Experiment 1, and the results for GOT, GPT and ALP are shown in Table 3 below. The results for MDA are shown in Table 4 below.

process Number GOT (IU / L) GPT (IU / L) ALP (IU / L) normal 10 60.7 ± 3.2 26.4 ± 6.4 62.7 ± 31.8 Ethanol (Control) 10 106.0 ± 5.1 42.0 ± 8.8 177.3 ± 27.3 Hwangchil Methanol Total Extract + Ethanol 10 91.6 ± 20.3 29.4 ± 6.7 156.8 ± 37.0 Crude Water Fraction + Ethanol 10 102.0 ± 23.8 30 ± 5.0 157.8 ± 53.0 Hwangchil Butanol Fraction + Ethanol 10 87.0 ± 16.9 27.5 ± 8.1 ^* 150.3 ± 69.3 Hwangchil Chloroform Fraction + Ethanol 10 91.5 ± 38.0 38.3 ± 5.1 169.3 ± 19.1 Hwangchil Hexane Fraction + Ethanol 10 86.0 ± 8.6 ^* 28.5 ± 6.73 ^* 158.0 ± 38.3

(*: p <0.05)

As can be seen in Table 3, even when the hwangchil extract and its fractions were administered before ethanol, as in the case of co-administration of Experimental Example 1 in the GOT for the hwangchil butanol total extract and its fraction administration group, all showed a significant decrease In the case of, GPT showed a significant decrease in both the group and the group treated with Hwangchil butanol fraction. In addition, ALP also showed a tendency to decrease in both the group and the fraction administration group.

As described above, it can be seen that both hwangchil extracts and their fractions exhibit markedly hepatocellular protective action, and that the hwangchil extracts and the fractions of the present invention can be used as a preventive agent of liver disease.

process Number MDA normal 10 0.0154 ± 0.002 Ethanol (Control) 10 0.0358 ± 0.001 Hwangchil Methanol Total Extract + Ethanol 10 0.0232 ± 0.003 ^* Crude Water Fraction + Ethanol 10 0.0222 ± 0.002 ^** Hwangchil Butanol Fraction + Ethanol 10 0.0361 ± 0.022 Hwangchil Chloroform Fraction + Ethanol 10 0.0200 ± 0.002 ^** Hwangchil Hexane Fraction + Ethanol 10 0.0209 ± 0.004 ^**

(*: p <0.05, **: p <0.01)

As shown in Table 4, even when the Hwangchil extract of the present invention and the fractions of the present invention, the concentration of MDA of hepatocytes was significantly reduced.

As described above, it can be seen that both hwangchil extracts and their fractions exhibit markedly hepatocellular protective action, and that the hwangchil extract of the present invention and its fractions can be used as a preventive agent of liver disease.

As described above, the hwangchil extract of the present invention or a fraction thereof has an excellent hepatocyte protective effect. Therefore, since the yellow lacquer extract of the present invention or a fraction thereof and a pharmaceutical composition comprising the same have an excellent hepatocyte protective effect without showing side effects, the present invention is intended to prevent and treat diseases related to hepatocyte protection such as fatty liver, hepatitis, cirrhosis, etc. It can be used in peace or in combination with a therapeutic agent of.

In addition, the hwangchil extract and fractions thereof of the present invention can be used as a medicine, drink or functional food for liver protection.

Claims (9)

Hwangchil extract having a hepatocyte protective effect extracted from the resin of the Hwangchil wood ( Dendropanax morbifera Lev.) With an alcohol solvent. The extract of claim 1, wherein the extract is a concentrated solution obtained by evaporating and condensing the supernatant obtained by cooling the extract obtained by adding Hwangchil resin to alcohol and warming it at 50 to 100 ° C for 5 to 24 hours at 40 to 60 ° C. Hwangchil extract having a hepatocyte protective effect. Hwangchil fraction having a hepatocyte protection effect is selected from the group consisting of hexane fraction, chloroform fraction, butanol fraction and water fraction obtained by sequentially repeatedly extracting the yellow lacquer extract of claim 1 as a solvent of hexane, chloroform, butanol and water. A composition having a hepatocyte protective effect, comprising the extract of claim 1 or 2, or the extract of claim 3 as an active ingredient. The pharmaceutical composition according to claim 4, wherein the composition comprises a composition and a pharmaceutically acceptable carrier. 6. The pharmaceutical composition according to claim 5, further comprising one or two or more additives selected from diluents, flavors, solubilizers, lubricants, suspensions, binders and disintegrants. The dietary supplement having hepatocellular protective effect according to claim 4, comprising the composition. 5. A composition according to claim 4, wherein said composition is a food composition. The composition of claim 8, wherein the food composition is selected from the group consisting of beverage, medicine, kimchi, yogurt, milk, ice cream, bread, rice cake and noodles.