KR20180055032A - Method for preparing soybean sources comprising nipa fruticans wurmb and soybean sources by the method - Google Patents
Method for preparing soybean sources comprising nipa fruticans wurmb and soybean sources by the method Download PDFInfo
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- KR20180055032A KR20180055032A KR1020160152311A KR20160152311A KR20180055032A KR 20180055032 A KR20180055032 A KR 20180055032A KR 1020160152311 A KR1020160152311 A KR 1020160152311A KR 20160152311 A KR20160152311 A KR 20160152311A KR 20180055032 A KR20180055032 A KR 20180055032A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
Description
본 발명은 간장, 된장, 고추장 및 쌈장 등과 같은 전통 장류의 제조방법에 관한 것으로서, 보다 상세하게는 니파야자 꽃대를 이용하여 관능성, 보존성 및 기능성이 향상된 저염화 장류를 제조하는 방법에 관한 것이다.More particularly, the present invention relates to a method for producing a low-boiled sweet potato having improved functionality, preservability and functionality by using a nifa palm phlox. The present invention relates to a method for producing a soy sauce such as soy sauce, miso, kochujang and sesame seed.
콩을 주원료로 하는 된장 및 간장과 같은 장류는 소금에서 오는 짠맛, 단백질 가수분해 산물인 아미노산에서 오는 구수한 맛, 발효에 의한 향 등이 조화를 이루어 독특한 향미와 색을 가지고, 단백질과 아미노산 함량이 높아 곡류 위주의 식생활에서 부족하기 쉬운 필수 아미노산 및 지방산, 유기산, 미네랄, 비타민류 등의 영양소를 보충해 주는 중요한 기능을 가진 식품이다.Soybean-based soybean paste and soy sauce, such as soy sauce, salty taste from salt, protein hydrolyzate, amino acid, salt and flavor fermented by harmony with a unique flavor and color, high protein and amino acid content It is a food having an important function to supplement nutrients such as essential amino acids and fatty acids, organic acids, minerals, and vitamins that are scarce in a grain-oriented diet.
그러나 전통장류는 보존성을 높이기 위하여 통상적으로 높은 농도의 소금을 첨가하므로, 고염 제품으로 분류되어 건강에 관심이 많은 소비자들이 기피하는 현상(고혈압 등 성인병의 원인)이 나타나고 있다.However, in order to enhance preservation, traditional herbs usually add a high concentration of salt, so they are classified as high salt products, and consumers who are interested in health are avoiding (cause of adult diseases such as hypertension).
실제로 세계보건기구(WHO)가 권장하는 하루 적정 소금 섭취량은 5 g이며, 반면 짜고 자극적인 음식을 선호하는 한국 성인의 하루 평균 소금 섭취량은 13.5 g으로 한국 성인의 소금 섭취량은 세계보건기구 권장량의 3배에 가깝다. 문제는 소금 섭취량의 대부분을 가공식품에서 얻는 서구 사람들과 달리 한국인들은 김치류와 장류 등 전통식단에서 소금을 절반 가까이 섭취한다는 것이며, 식품의약품안전처는 나트륨 섭취의 주요급원을 김치류(25%), 장류(22%), 소금(20%) 순으로 명시하였다.In fact, the World Health Organization (WHO) recommends a daily salt intake of 5 g, while Korean adults who prefer salty and intolerant food have a daily average salt intake of 13.5 g, It is near the ship. The problem is that Koreans eat almost half of their salt in traditional diets such as kimchi and soup, unlike the western people who get most of their salt intake from processed foods. The Food and Drug Administration's main source of sodium intake is kimchi (25%), (22%), and salt (20%).
이처럼 식생활 패턴이 현대에 와서 많이 변화되어 가고 있으며, 그에 따라 국민이 가장 많이 쓰고 있는 식재료의 저염화 필요성이 대두되고 있다. 따라서 저장성을 위한 장류의 과다한 식염 사용은 과도한 짠맛과 함께 고혈압, 뇌졸중, 위암, 신장병, 간경변증, 만성신부전증 등 성인병을 유발하므로 기호성 향상과 성인병 예방을 위해 장류의 식염 함량을 낮출 필요가 있다.As such, the pattern of eating habits has changed a lot in the modern times, and the necessity of low chlorination of the food materials which the people use the most is emerging. Therefore, excessive use of salt for preservation may cause excessive diseases such as hypertension, stroke, stomach cancer, kidney disease, cirrhosis, chronic renal failure and so on. Therefore, it is necessary to lower the salt content of soy sauce to improve palatability and prevent adult diseases.
한편, 저염장류 제품의 제조시 필연적으로 발생하는 보존성 문제를 해결하기 위한 수단으로서 에탄올 첨가, 감마선 조사와 같은 다양한 물리적, 화학적 방법들이 연구된 바 있으며, 이중 산업현장에서 적용되고 있는 기술로는 물리적 방법으로 저온살균 방법(대한민국특허 제1989-4274호)이 있고, 화학적 방법으로 95% 주정처리 및 겨자고추냉이 첨가 등의 방법(대한민국특허 제1219321호)이 현실화되어 있다. 하지만 이러한 방법들은 전통장류제품에 염도를 저하시키는데 일부 효과적이나 맛과 향미의 저하가 필연적으로 수반되어 왔다.On the other hand, various physical and chemical methods such as ethanol addition and gamma irradiation have been studied as a means for solving the preservation problem that necessarily occurs in the production of low salt products, (Korean Patent No. 1989-4274), and a method such as 95% alcohol treatment and mustard horseradish addition by chemical method (Korean Patent No. 1219321) has been realized. However, these methods have been effective in lowering the salinity of traditional herbal products, but have inevitably accompanied deterioration of taste and flavor.
또한 최근에 된장의 항암효과, 항 돌연변이 효과, 면역기능 강화 효과, 항고혈압 효과, 혈전용해 효과, 콜레스테롤 저하 효과, 항산화 효과 등 다양한 기능성이 밝혀져 새롭게 관심을 끌고 있으며, 나아가, 된장성분에 천연물의 효능을 부가하여 기능성을 향상시키는 연구가 활발하다.Recently, various functionalities such as anticancer effect, antimutagenic effect, immune function enhancing effect, antihypertensive effect, thrombolytic effect, cholesterol lowering effect and antioxidative effect have recently been attracted attention. Furthermore, To improve the functionality.
일례로서 대한민국 등록특허 제10-1105165호에서는 돼지감자를 이용하여 장류를 개발하였으며, 대한민국 공개특허 제10-2005-0010914호에서는 조릿대를 이용하여 장류를 개발하였다. For example, in Korean Patent No. 10-1105165, a sweet potato was used to develop a sweet potato, and in Korean Patent Publication No. 10-2005-0010914, a sweet pot was developed using a sweet potato.
이에, 본 발명자들은 전통장류의 저염화와 장류의 고유기능에 추가 성분의 효능을 부가하여 고부가가치의 기능성 장류를 제공하고자 노력한 결과, 니파야자의 꽃대가 저염화 및 보존성 증진이 가능하도록 한다는 점을 확인함으로써, 본 발명을 완성하였다. Accordingly, the present inventors have made efforts to provide high-value-added functional soy sauce by adding the effect of an additional ingredient to the intrinsic function of the traditional soy sauce and the low soy sauce, and as a result, By confirming, the present invention has been completed.
본 발명의 목적은 전통 장류의 저염화와 장류의 고유기능에 추가 성분의 효능을 부가함으로써 고부가가치를 구현한 기능성 장류의 제조방법을 제공하는 것이다.It is an object of the present invention to provide a method for producing functional soy sauce having high added value by adding the effect of an additional ingredient to the inherent functions of traditional soy sauce and low soy sauce.
또한, 저염화와 장기 보관안정성이 확보된 장류를 제공하는 것이다.In addition, it is intended to provide a safflower which has low chlorination and long-term storage stability.
상기 목적을 달성하기 위하여, 본 발명은 간장, 된장, 고추장 및 쌈장으로 이루어지는 군으로부터 선택된 장류의 제조방법에 있어서, 니파야자 꽃대의 추출물, 니파야자 꽃대 분말 또는 2종 모두를 첨가하여 제조하는 것을 특징으로 한다. In order to achieve the above object, the present invention provides a method for producing a variety of products selected from the group consisting of soy sauce, miso, kochujang and sesame seeds, characterized in that the extract is prepared by adding an extract of nifa palm phlox, .
물 100중량부, 니파야자 꽃대의 추출물 20~40중량부를 포함하며, 염도가 16~18%(w/w)인 염수를 제조하는 단계와, 상기 제조된 염수에 메주를 투입하는 단계와, 상기 메주가 포함된 염수 100중량부에 니파야자 꽃대 분말 1~10중량부를 투입하는 단계와, 상기 니파야자 꽃대 분말이 투입된 혼합물을 정치하여 1~2년간 숙성시키는 단계와, 상기 숙성된 숙성물로부터 간장과 된장을 얻는 단계를 포함하는 것을 특징으로 한다.100 parts by weight of water, 20-40 parts by weight of an extract of the nifa palm phlox, and having a salinity of 16 to 18% (w / w), adding Meju to the prepared saline solution, 1 to 10 parts by weight of a nifa palm phalaenopsis powder is added to 100 parts by weight of a brine containing meju, and the mixture is aged for 1 to 2 years, And a step of obtaining the maltose.
물 100중량부, 엿기름 5~10중량부, 니파야자 꽃대 추출물 5~10중량부를 혼합한 후, 1~3시간 동안 당화시켜 상등액을 수득하는 단계와, 찹쌀 또는 멥쌀가루에 물을 투입하고 가열하여 풀을 제조하는 단계와, 상기 수득된 상등액에 상기 제조된 풀을 투입하여 3~7시간 당화시키는 단계와, 상기 당화된 당화액 100중량부에 고추가루 5~10중량부, 메주가루 5~10중량부, 소금 1~2중량부 및 니파야자 꽃대 분말 1~10중량부를 혼합하는 단계와, 상기 혼합된 혼합물을 15~20℃에서 1~2개월간 숙성시켜 고추장을 얻는 단계를 포함하는 것을 특징으로 한다.Mixing 5 to 10 parts by weight of water, 5 to 10 parts by weight of maltose and 5 to 10 parts by weight of an extract of Nephala palmate, and saccharifying the mixture for 1 to 3 hours to obtain a supernatant; adding water to glutinous rice or rice flour and heating A step of adding the above-prepared paste to the obtained supernatant to saccharify for 3 to 7 hours, and a step of adding 5 to 10 parts by weight of red pepper powder, 5 to 10 parts by weight of meju powder to 100 parts by weight of the saccharified saccharified liquid, 1 to 2 parts by weight of salt and 1 to 10 parts by weight of a nifa palm phalaenopsis powder; and aging the mixed mixture at 15 to 20 ° C for 1 to 2 months to obtain kochujang do.
고추장 100중량부에 된장 50~100중량부, 니파야자 꽃대 추출물 5~10중량부, 니파야자 꽃대 분말 20~50중량부를 혼합하여 쌈장을 얻는 단계를 포함하는 것을 특징으로 한다. 50 to 100 parts by weight of miso, 5 to 10 parts by weight of a nifel palm phlox, and 20 to 50 parts by weight of a nifa palm phalaenopsis powder are mixed with 100 parts by weight of a red pepper paste to obtain a sesame paste.
상기 니파야자 꽃대 추출물은, 니파야자의 꽃대를 물을 용매로 하여 열수추출 또는 초음파추출한 것이고, 상기 니파야자 꽃대 분말은, 니파야자 꽃대를 절단하여 물에 데친 후, 탈수, 건조 및 분쇄한 것임을 특징으로 한다. The above-mentioned nifa palm phloem extract is obtained by extracting with hot water or ultrasonically extracting the palm of Nipaya with water as a solvent. The nifa palm phalaenopsis is obtained by cutting the nipponia palm flower and pouring it in water, followed by dehydration, drying and pulverization .
상기 니파야자 꽃대 분말을 혼합할 시, 홍화씨 분말 1~3중량부를 더 혼합하는 것을 특징으로 한다. And 1 to 3 parts by weight of safflower seed powder is further mixed when the nifa palm phalaenopsis powder is mixed.
그리고 본 발명에 의한 기능성 장류는 상기한 방법으로 제조되는 것을 특징으로 한다.The functional soybean paste according to the present invention is characterized by being manufactured by the above-mentioned method.
본 발명에 의하면 니파야자 꽃대에 의해 항산화의 기능성을 갖는 장류의 제조가 가능할 뿐 아니라, 보존성이 우수한 저염화 장류의 제조가 가능하다는 장점이 있다.According to the present invention, there is an advantage that it is possible not only to produce an intestinal flora having antioxidative function by means of the nifa palm phlox, but also to produce a low-concentration intestinal flora excellent in preservability.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
먼저, 본 발명에 따른 기능성의 장류는, 간장, 된장, 고추장 및 쌈장 중 1종 이상의 것을 의미하며, 기능성이란 항산화성을 의미한다.First, the functional soy sauce according to the present invention means at least one of soy sauce, soybean paste, red pepper sauce, and sesame sauce, and the function means antioxidative property.
본 발명의 가장 큰 특징은 상기 장류의 제조시, 니파야자의 꽃대 추출물, 분말 또는 이들 모두를 첨가하여 제조함으로써, 저염 장류를 제조하더라도 그 보존성이 우수하고, 기능성이 우수하며, 풍미 역시 우수한 것이다.The most remarkable feature of the present invention is that, when the above-mentioned variety is manufactured, it is prepared by adding the extract of Phyllostachys japonica, powder or all of them, even when a low salt content is produced, the preservability is excellent, the function is excellent and the flavor is also excellent.
여기서, 상기 니파야자(학명 : NIPA FRUTICANS WURMB)는 인도, 말레이시아를 비롯한 동남아시아 및 오스트레일리아가 원산지이며, 맹그로브 지대 등의 습지에서 자란다. 뿌리는 땅속에서 여러 개로 갈라지며, 잎은 지면에서 뭉쳐나고 광택이 있는 녹색을 띤다. 그리고 꽃은 암수한그루이며, 지면의 잎겨드랑이에서 나오는데, 보통 10월에서 이듬해 6월 사이에 꽃대가 올라온다. Here, the above-mentioned NIPPA FRUTICANS WURMB is originated from India, Malaysia, Southeast Asia and Australia, and grows in wetlands such as mangroves. The roots are divided into several pieces in the ground, and the leaves are gathered on the ground and have a glossy green color. And the flower is one male and one female, and it comes out from the axilla of the ground. Usually, the flower stands rise from October to June of the following year.
본 발명은 이러한 니파야자의 꽃대를 채취하고, 이를 가공하여 추출물 또는 분말로서 제조하는 것인데, 특히 니파야자의 꽃대를 사용하는 이유는 니파야자의 다른 어떤 부위보다 꽃대에 폴리페놀 등의 항산화 성분이 풍부하고, 셀레늄 등의 각종 영양성분이 풍부할 뿐 아니라, 우수한 항염증 효능을 보이기 때문이다. 또한, 니파야자의 꽃봉오리에도 인체 유익성분이 다량 함유되어 있으므로, 꽃대에 형성된 꽃봉오리를 제거하지 않고 그대로 사용할 수 있음은 당연하다.The present invention is based on the fact that the flower buds of Nipaya are used for producing extracts or powders, and the reason for using the flower buds of Nipaya is that the flower buds are rich in antioxidant components such as polyphenols It is rich in various nutrients such as selenium, and exhibits excellent anti-inflammatory effects. In addition, since the flower bud of nipayanasia contains a large amount of human-beneficial ingredients, it is natural that the flower bud formed in the flower bud can be used without being removed.
상기 니파야자 꽃대의 성분분석 및 검사 결과를 하기 표 1 및 표 2에 나타내었는바, 상기 니파야자의 꽃대는 폴리페놀, 플라보노이드, 사포닌 등은 물론, 아스파르트산, 티로신, 히스티딘 등의 유효성분을 다량 함유한다. The results of the analysis and the results of the components of the above-mentioned Nihonpalaceae are shown in the following Tables 1 and 2. As shown in Table 1 and Table 2, the Niha-Paya's peduncle contains a large amount of effective components such as polyphenol, flavonoid, saponin, as well as aspartic acid, tyrosine and histidine .
따라서, 이러한 니파야자 꽃대의 추출물 또는 분말을 이용하면, 장류에 우수한 항산화력이 부여되어 인체에 유익한 효과를 부여하게 되며, 장류의 전체적인 풍미를 개선하는 것은 물론, 보존성을 개선해준다.Therefore, the use of the extract or powder of the nifa palm phlox is imparted with an excellent antioxidant power to the soup, thereby giving a beneficial effect to the human body, improving not only the overall flavor of the soup, but also the preservability.
여기서, 상기 니파야자 꽃대의 추출물은 물을 용매로 하여 열수추출 또는 초음파 추출한 것을 의미한다.Herein, the extract of the above-mentioned Nipah palm phalanx means hot water extraction or ultrasonic extraction using water as a solvent.
보다 구체적으로는, 니파야자의 꽃대를 3~10cm 정도의 크기로 절단하고, 상기 절단된 니파야자의 꽃대를 물을 용매로 하여 열수추출 또는 초음파 추출한 후, 이를 농축하며, 필요에 따라 건조하여 분말화한다. 여기서, 상기 건조는 동결 건조 또는 분무 건조 등을 이용할 수 있으며, 이는 이 기술이 속하는 분야에서 공지된 방법을 따르므로, 이에 대한 상세한 설명은 생략한다.More specifically, the flower bud of Nipaya is cut to a size of about 3 to 10 cm, and the flower bud of the cut nipaya is subjected to hot water extraction or ultrasonic extraction using water as a solvent, which is then concentrated, I am angry. Here, the drying may be performed by lyophilization, spray drying, or the like, which is well known in the art, and therefore, a detailed description thereof will be omitted.
상기 열수추출이란, 상기 니파야자의 꽃대에 10~20중량배 정도의 물을 가한 후, 80~100℃의 온도에서 10~20시간 추출하는 것이고, 초음파 추출은 니파야자 꽃대에 8~12중량배의 물을 가한 후, 초음파 추출기를 이용하여 100~150kHz 정도의 조건으로 20~30분간 전처리하고, 이를 다시 50~60℃의 온도에서 열수추출을 10~12시간 하는 것이다.The hot water extraction is performed by adding 10 to 20 times by weight of water to the spikelets of the nipayanas and then extracting them at a temperature of 80 to 100 ° C for 10 to 20 hours. The ultrasonic extraction is performed at 8 to 12 times And the mixture is pre-treated for 20 to 30 minutes under the condition of about 100 to 150 kHz by using an ultrasonic wave extractor, and further subjected to hot water extraction for 10 to 12 hours at a temperature of 50 to 60 ° C.
또한, 추출효율을 높이기 위하여 상기 절단된 니파야자를 자연건조, 냉풍건조 또는 열풍건조하여 함수율 10~20%가 되도록 한 후, 이를 100~200mesh 정도로 분쇄하고, 이러한 분말로부터 유효성분을 추출할 수도 있다. 아울러, 상기 절단된 니파야자 꽃대를 100℃의 끓는 물에 10~20분간 데친 후, 상기 데친 꽃대를 탈수하고, 이로부터 유효성분을 추출할 수도 있다.In order to increase the extraction efficiency, the cut nipaya is subjected to natural drying, cold air drying or hot air drying to have a water content of 10 to 20%, followed by pulverizing the dried powder to about 100 to 200 mesh, and extracting the active ingredient from the powder . In addition, it is also possible to dehydrate the cut nippa coconut palms in boiling water at 100 ° C for 10 to 20 minutes, dehydrate them, and extract the active ingredient therefrom.
그리고 상기 농축은 감압 농축하는 것을 의미하는 것으로, 50~60℃에서 용매를 제거하여 20~50Brix 정도로 농축하는 것이다. 또한, 필요에 따라 농축 전, 여과할 수 있음은 당연하다.The concentration means concentration under reduced pressure, and the solvent is removed at 50 to 60 ° C to concentrate the solution to about 20 to 50 Brix. It is of course also possible to conduct filtration before concentration, if necessary.
아울러, 상기 니파야자의 꽃대 분말은, 니파야자 꽃대를 절단하는 단계와, 상기 절단된 니파야자 꽃대를 80~100℃의 물에 20~40분간 데치는 단계와, 상기 데친 니파야자 꽃대를 탈수하는 단계와, 상기 탈수된 니파야자 꽃대를 건조하는 단계와, 상기 건조된 꽃대를 200~10,000mesh로 분쇄하는 단계를 포함한다. In addition, the phalaenopsis powder of Nipaya can be prepared by cutting a nifa palm flower bed, a step of dipping the cut nipa palm flower in water at 80 to 100 ° C for 20 to 40 minutes, a step of dehydrating the wetted nipa palm flower Drying the dehydrated Niphalia phalaenopsis, and pulverizing the dried phalaenopsis to 200 to 10,000 mesh.
즉, 먼저 니파야자의 꽃대를 채취하고 깨끗이 세척한 후, 이를 3~10cm의 길이로 절단한다. 다음으로, 상기 절단된 꽃대를 고온의 물, 즉 80~100℃의 물에 20~40분간 데쳐내고, 상기 데친 꽃대를 찬물에 담가 식힌 후, 길이방향, 즉, 결대로 길게 찢는다. 이때, 그 두께는 대략 0.5~1cm 정도가 바람직하다. 상기 니파야자의 꽃대는 길이방향으로 나란한 결을 가지기 때문에 쉽게 결대로 쉽게 찢어진다. 다음으로, 상기 찢은 꽃대에 탈수하고, 열풍건조, 냉풍건조 또는 일광에 의한 자연건조법을 통해 함수율이 5~10%가 되도록 건조한 후, 상기 건조된 꽃대를 200~10,000mesh로 분쇄기 또는 제분기를 이용하여 분쇄함으로써, 그 제조를 완료한다. 본 발명에서 상기 탈수방법은 제한하지 않는 것으로, 별도의 탈수장치를 이용하거나, 손으로 눌러 탈수할 수 있다.In other words, first take a flower bud of Nipaya, clean it, and cut it to a length of 3 ~ 10cm. Next, the cut flower stalks are heated in hot water, that is, water at 80 to 100 ° C for 20 to 40 minutes, and the dulled flower buds are soaked in cold water and then ripped in the longitudinal direction, At this time, the thickness is preferably about 0.5 to 1 cm. The nipayanaya peduncle is easily torn as easily because it has parallel longitudinal texture. Next, the tears are dewatered, dried by hot air drying, cold air drying, or natural drying method by daylight to dry to a moisture content of 5 to 10%, and then dried at 200 to 10,000 mesh using a pulverizer or mill Followed by pulverization, thereby completing the production. In the present invention, the dewatering method is not limited, and a separate dewatering device may be used or dewatered by hand.
이하, 본 발명을 상세히 설명한다. 이하에서의 니파야자 꽃대 추출물과 니파야자 꽃대 분말은 상기에서 설명한 바와 같이 제조된 것을 의미한다.Hereinafter, the present invention will be described in detail. Hereinafter, the term " nifa palm phloem extract " and " nifa palm phalaenopsis powder " means those prepared as described above.
먼저, 본 발명에 따른 간장 및 된장의 제조방법은, 보다 구체적으로 물 100중량부, 니파야자 꽃대의 추출물 20~40중량부를 포함하며, 염도가 16~18%(w/w)인 염수를 제조하는 단계와, 상기 제조된 염수에 메주를 투입하는 단계와, 상기 메주가 포함된 염수 100중량부에 니파야자 꽃대 분말 1~10중량부를 투입하는 단계와, 상기 니파야자 꽃대 분말이 투입된 혼합물을 정치하여 1~2년간 숙성시키는 단계와, 상기 숙성된 숙성물로부터 간장과 된장을 얻는 단계를 포함한다.More specifically, the method for producing soy sauce and soybean paste according to the present invention comprises 100 parts by weight of water and 20 to 40 parts by weight of an extract of the nifa palm phlox, wherein the salt water having a salinity of 16 to 18% (w / w) Adding 1 to 10 parts by weight of a nifa palm phalaenopsis powder to 100 parts by weight of the brine containing the meju; And aging for 1 to 2 years, and obtaining soy sauce and soybean paste from the aged aged product.
물 water 100중량부100 parts by weight , , 니파야자Nipa 꽃대의 추출물 20~ Extract of flower bud 20 ~ 40중량부를40 parts by weight 포함하며, 염도가 16~18%(w/w)인 염수를 제조하는 단계. (W / w), and having a salinity of 16 to 18% (w / w).
즉, 먼저, 물, 니파야자 꽃대의 추출물 및 천일염을 이용하여 염수를 제조하는데, 통상 장류의 보존성을 높이기 위하여 17~21%의 염도가 요구되나, 본 발명에서는 염도를 낮추더라도 니파야자 꽃대를 통해 보존성을 높일 수 있으므로, 저염도, 즉 16~18%(w/w)인 염수를 제조하여 저염화를 구현할 수 있다. Namely, salt water is first prepared using water, extracts of Nihon palaceae, and salt of sunflower. Normally, salinity is required to be 17 ~ 21% in order to enhance the preservability of the seaweed. However, in the present invention, Since the preservability can be enhanced, low salt can be achieved by producing low salt, that is, salt water having 16 to 18% (w / w).
여기서, 상기 니파야자 꽃대의 추출물은 염도를 낮추는 역할은 물론, 기능성을 부여하고, 풍미를 좋게 하며, 보존성을 높이는 역할을 하는 것으로, 물 100중량부에 대하여 니파야자 꽃대의 추출물 20~40중량부를 혼합한다. 이때, 상기 추출물이 20중량부 미만이면 그 역할이 미미하고, 40중량부를 초과하면 장류 고유의 풍미를 저하시킬 수 있기 때문이다.Herein, the extract of the Nihonbara phalaenopsis serves not only to lower salinity, but also to impart functionality, improve flavor and enhance preservability. To 100 parts by weight of water is added 20 to 40 parts by weight of an extract of Nihon palaceae Mix. At this time, if the extract is less than 20 parts by weight, the function thereof is insignificant. If the extract is more than 40 parts by weight, the inherent flavor of the soybean paste can be lowered.
상기 제조된 염수에 메주를 투입하는 단계.And introducing Meju into the brine prepared above.
다음으로, 상기 제조된 염수에 메주를 투입한다. Next, Meju is added to the above prepared brine.
여기서, 상기 메주의 종류는 제한하지 않으며, 그 사용량은 제조된 염수와 1:0.1~0.2중량비 정도이면 족하다.Here, the type of meju is not limited, and the amount of meju may be about 1: 0.1 ~ 0.2 weight ratio with the salt water produced.
상기 메주가 포함된 염수 The Meju-containing brine 100중량부에100 parts by weight 니파야자Nipa 꽃대 분말 1~ Phalaenopsis powder 1 ~ 10중량부를10 parts by weight 투입하는 단계. Step of injecting.
다음으로, 상기 메주가 포함된 염수 100중량부에 대하여 1~10중량부의 니파야자 꽃대 분말을 투입한다. 상기 니파야자 꽃대의 분말을 염수에 혼합함으로써, 항산화성, 보존성 및 풍미를 개선해 주는바, 그 함량이 너무 적으면 그 역할이 미미하고, 과량이 되면 오히려 장류 특유의 풍미가 나빠지므로, 1~10중량부만큼 투입한다.Next, 1 to 10 parts by weight of the nifalcaya peduncle powder is added to 100 parts by weight of the brine containing meju. When the content of the nifa palm phalanx is mixed with saline water, the antioxidative property, preservability and flavor are improved. If the content is too small, the role is insignificant. If it is excessive, Put in by weight.
상기 remind 니파야자Nipa 꽃대 분말이 투입된 혼합물을 정치하여 1~2년간 숙성시키는 단계. Stirring the mixture containing the pedicel powder and aging for 1 ~ 2 years.
그리고 상기 니파야자 꽃대 분말이 투입된 혼합물을 10~30℃의 온도에서 1~2년간 숙성시킨다. 이 과정은 통상의 장류 제조방법에 의한 것이므로, 이에 대한 상세한 설명은 생략한다.Then, the mixture containing the above-mentioned Nipahaya phalaenopsis powder is aged at a temperature of 10 to 30 DEG C for 1 to 2 years. Since this process is performed by a conventional soybean curd production method, a detailed description thereof will be omitted.
상기 숙성된 The aged 숙성물로부터From aged material 간장과 된장을 얻는 단계. Steps to obtain soy sauce and miso.
다음으로, 이러한 숙성물로부터 고형상과 액상을 분리하여, 된장과 간장을 수득한다.Next, solid form and liquid form are separated from the aged material to obtain doenjang and soy sauce.
상기와 같은 방법을 통해 수득한 된장과 간장은 니파야자 꽃대의 유효성분이 풍부하여 기능성이 우수하고, 풍미가 우수하며, 저염도임에도 보존성이 우수하다는 장점이 있다.The soybean paste and soy sauce obtained through the above method are rich in effective ingredients of the nipponophyllum phalaenopsis, so that they are excellent in functionality, excellent in flavor and excellent in preservability even in low saltiness.
이하, 본 발명에 따른 고추장의 제조방법을 상세히 설명한다.Hereinafter, a method of manufacturing a kochujang according to the present invention will be described in detail.
본 발명에 따른 고추장의 제조방법은, 보다 상세하게 물 100중량부에 대해 엿기름 5~10중량부, 니파야자 꽃대 추출물 5~10중량부을 혼합한 후, 1~3시간 동안 당화시켜 상등액을 수득하는 단계와, 찹쌀 또는 멥쌀가루에 물을 투입하고 가열하여 풀을 제조하는 단계와, 상기 수득된 상등액에 상기 제조된 풀을 투입하여 3~7시간 당화시키는 단계와, 상기 당화된 당화액 100중량부에 고추가루 5~10중량부, 메주가루 5~10중량부, 소금 1~2 중량부 및 니파야자 꽃대 분말 1~10중량부를 혼합하는 단계와, 상기 혼합된 혼합물을 15~20℃에서 1~2개월간 숙성시키는 단계를 포함한다.The method of manufacturing a kochujang according to the present invention is characterized in that 5 to 10 parts by weight of malt is mixed with 5 to 10 parts by weight of an extract of Nipahaya phalaenopsis to 100 parts by weight of water and then saccharification is performed for 1 to 3 hours to obtain a supernatant Adding water to the glutinous rice flour or rice flour to prepare a glutinous rice flour; heating the glutinous rice flour or glutinous rice flour to prepare a glutinous rice flour; heating the resulting glutinous rice flour to 3 to 7 hours; 5 to 10 parts by weight of red pepper powder, 5 to 10 parts by weight of meju powder, 1 to 2 parts by weight of salt and 1 to 10 parts by weight of nifalaxanthin powder are mixed, And aging for 2 months.
물 water 100중량부100 parts by weight , 엿기름 5~, Malt 5 ~ 10중량부10 parts by weight , , 니파야자Nipa 꽃대 추출물 5~ Flower bud extract 5 ~ 10중량부를10 parts by weight 혼합한 후, 1~3시간 동안 After mixing, after 1 to 3 hours 당화시켜Sugar 상등액을 수득하는 단계. To obtain a supernatant.
먼저, 물, 엿기름, 니파야자 꽃대 추출물을 혼합한다. 여기서, 그 혼합비는 물 100중량부에 대해 엿기름 5~10중량부, 니파야자 꽃대 추출물 5~10중량부로 혼합한다. 여기서, 상기 니파야자 꽃대 추출물은 고추장의 풍미를 개선하고, 보존성을 개선해주는 역할을 하는바, 그 함량이 너무 적으면 그 역할이 미미하고, 과량이 되면 오히려 풍미가 나빠진다.First, water, malt, and nipah palm phloem extract are mixed. Here, the mixing ratio thereof is 5 to 10 parts by weight of malt with respect to 100 parts by weight of water, and 5 to 10 parts by weight of the extract of Nephala palmetto. Here, the above-mentioned Nipah palm phloem extract improves the flavor of the koji paste and improves the preservability. If the content is too small, its role is insignificant, and if it is excessive, the flavor deteriorates.
그리고 상기 혼합물을 20~60℃에서 1~3시간 동안 당화시켜 상등액을 수득한다.And the mixture is saccharified at 20 to 60 ° C for 1 to 3 hours to obtain a supernatant.
찹쌀 또는 멥쌀가루에 물을 투입하고 가열하여 풀을 제조하는 단계.Water is added to glutinous rice or rice flour and heated to produce a paste.
그리고 상기 상등액과는 별도로, 찹쌀 또는 멥쌀가루에 2~4중량배의 물을 가하고, 80~100℃로 10~60분간 가열하여 풀을 제조한다. Separately from the supernatant, 2 to 4 times by weight of water is added to glutinous rice flour or rice flour, and the mixture is heated at 80 to 100 ° C for 10 to 60 minutes to prepare a paste.
상기 remind 수득된The obtained 상등액에 상기 제조된 풀을 투입하여 3~7시간 The above prepared paste was added to the supernatant and the mixture was stirred for 3 to 7 hours 당화시키는Glycosylated 단계. step.
다음으로, 상기 수득된 상등액에 상기 제조된 풀을 투입하여 3~7시간 당화시킨다. 여기서, 상기 당화온도는 50~60℃면 족하며, 상기 상등액에 대한 풀의 투입량은 1:1~2중량비 정도면 족하다. 그리고 상기 당화시킨 당화액은 그 부피가 1/2이 될 정도로 농축한다.Next, the thus-prepared paste is added to the obtained supernatant and saccharified for 3 to 7 hours. The saccharification temperature may be in the range of 50 to 60 ° C, and the amount of the paste added to the supernatant may be about 1: 1 to 2: 1. The saccharified saccharide solution is concentrated to a volume of 1/2.
상기 remind 당화된Glycosylated 당화액에To the saccharified solution 고추가루, Chili powder, 메주가루Meju flour , 소금 및 , Salt and 니파야자Nipa 꽃대 분말을 혼합하는 단계. Step of blending the pedicel powder.
다음으로, 상기 당화된 당화액 100중량부에 고추가루 5~10중량부, 메주가루 5~10중량부, 소금 1~2 중량부 및 니파야자 꽃대 분말 1~10중량부를 혼합한다.Next, 5 to 10 parts by weight of red pepper powder, 5 to 10 parts by weight of meju powder, 1 to 2 parts by weight of salt and 1 to 10 parts by weight of nifalaxy powder are added to 100 parts by weight of the saccharified saccharified liquid.
여기서, 상기 고추가루, 메주가루 및 소금은 통상 사용하는 재료로서, 그 배합비 역시 공지의 방법에 따른다. 다만, 소금은 종래 공지된 배합비의 50~80% 정도 수준으로, 저염화를 위한 것이다.Herein, the red pepper powder, meju powder, and salt are usually used as materials, and their blending ratios are also known methods. However, the salt is about 50 to 80% of the conventionally known mixing ratio, and is intended for low chloride.
그리고 상기 니파야자 꽃대 분말은 고추장의 기능성 및 저장성을 높이고 풍미를 개선하기 위한 것으로, 그 함량이 너무 적으면 효과가 미미하고 10중량부를 초과하면 과량이 되어 오히려 풍미가 나빠진다. If the content is too small, the effect is insignificant. If the content is more than 10 parts by weight, the amount of the nipa palm phalaenopsis is excessive and the flavor is deteriorated.
상기 혼합된 혼합물을 15~20℃에서 1~2개월간 숙성시키는 단계.Aging the mixed mixture at 15 to 20 ° C for 1 to 2 months.
최종적으로 상기 혼합물을 15~20℃ 정도에서 1~2개월간 숙성시켜 고추장을 완성한다.Finally, the mixture is aged at about 15 to 20 DEG C for 1 to 2 months to complete the kochujang.
상기와 같이 제조된 고추장은 그 풍미가 우수하고, 저염화에도 불구하고 기능성 및 보존성이 우수하다는 특징이 있다.The prepared kochujang is characterized by its excellent flavor and excellent functionality and preservability in spite of its low chlorination.
이하, 쌈장의 제조방법에 대해 상세히 설명한다.Hereinafter, a method for producing sesame seed will be described in detail.
본 발명에 따른 쌈장의 제조방법은, 고추장, 된장, 니파야자 꽃대 추출물, 니파야자 꽃대 분말을 혼합하는 단계를 포함한다.The method of manufacturing a sesame seed according to the present invention includes mixing a koji paste, a doenjang, a nihama palm phloem extract, and a nihon palm phalaenopsis powder.
즉, 보다 구체적으로는 통상의 고추장 100중량부에 된장 50~100중량부, 니파야자 꽃대 추출물 5~10중량부, 니파야자 꽃대 분말 20~50중량부를 혼합하고, 이에 부재료로서 마늘, 양파, 생강, 고추가루 및 참기름을 혼합하여 제조한다. 여기서, 상기 부재료의 배합비는 제한하지 않으며, 마늘, 생강, 양파는 다져서 사용함은 당연하다. 아울러, 부재료의 혼합은 생략할 수 있다. More specifically, 50 to 100 parts by weight of doenjang, 5 to 10 parts by weight of the extract of Nihon palaceae, and 20 to 50 parts by weight of the naphthalene palatinar powder are mixed in 100 parts by weight of a typical kochujang, and garlic, onion, ginger , Red pepper powder and sesame oil. Here, the compounding ratio of the above-mentioned ingredients is not limited, and garlic, ginger, and onion are used intensively. In addition, the mixing of the sub materials can be omitted.
상기와 같은 쌈장은, 니파야자 꽃대 추출물과 분말을 다량 포함함으로써, 저염화가 가능한 것은 물론, 보존성 및 기능성, 풍미가 우수하다는 특징이 있는바, 니파야자 꽃대 추출물과 분말의 함량이 너무 적으면 그 역할이 미미하고, 과량이 되면 풍미가 나빠지기 때문이다.The above-mentioned Samjang is characterized in that it contains not only low-salt but also excellent preservability, functionality and flavor by containing a large amount of the extract of Nihon palm phloem and powder, and when the content of the extract is high, This is because it is insignificant, and when it becomes excessive, the flavor becomes worse.
또한, 상기 고추장 및 된장은 통상 시판되는 상품을 구입하여 사용하거나, 집에서 직접 제조한 고추장, 된장을 활용할 수도 있는 것으로, 그 사용을 제한하지 않는다. 아울러, 앞서 설명한 본 발명에 따른 니파야자 꽃대를 이용한 기능성의 추출물과 분말을 이용할 수도 있음은 당연하다. The kochujang and doenjang can be obtained by purchasing commercially available commodities or by using home-made kochujang and miso, and the use thereof is not limited. It is of course also possible to use functional extracts and powders of the present invention as described above.
한편, 상기 된장, 간장, 고추장 및 쌈장의 제조시, 니파야자의 꽃대 분말을 투입할 때, 즉, 된장 및 간장의 제조시 상기 메주가 포함된 염수 100중량부에 니파야자 꽃대 분말 1~10중량부를 투입하는 단계, 또는 고추자의 제조시 상기 당화된 당화액 100중량부에 고추가루 5~10중량부, 메주가루 5~10중량부, 소금 1~2 중량부 및 니파야자 꽃대 분말 1~10중량부를 혼합하는 단계, 또는 쌈장의 제조시 상기 고추장 100중량부에 된장 50~100중량부, 니파야자 꽃대 추출물 5~10중량부, 니파야자 꽃대 분말 20~50중량부를 혼합하는 단계에서 홍화씨 분말 1~3중량부를 각각 더 혼합할 수도 있는바, 상기 홍화씨 분말을 더 포함하면 저염화에 더욱 도움이 됨은 물론, 보존성 역시 더 개선될 수 있기 때문이다.Meanwhile, when preparing the soybean paste, soy sauce, red pepper paste, and sesame seed, when the pulp powder of Nipaya is added, that is, when the soybean paste and the soy sauce are prepared, 100 parts by weight of the Meju- 5 to 10 parts by weight of red pepper powder, 5 to 10 parts by weight of meju powder, 1 to 2 parts by weight of salt, and 1 to 10 parts by weight of nifalaxanthin powder are added to 100 parts by weight of the saccharified saccharified liquid at the time of preparation of red pepper powder Mixing 50 to 100 parts by weight of doenjang, 5 to 10 parts by weight of a nifa palm phalaenopsis extract and 20 to 50 parts by weight of a nifa palm phalaenopsis powder with 100 parts by weight of the koji paste in the preparation of a sesame seed, 3 parts by weight of the safflower seed powder may be further mixed, and the safflower seed powder may further be added to the safflower seed powder.
이하, 본 발명을 하기의 실싱예를 통해 더욱 상세히 설명한다.Hereinafter, the present invention will be described in more detail with reference to the following embodiments.
(실시예 1:니파야자 꽃대 추출물의 제조)(Example 1: Preparation of Extract of Isolate Nymphalidae)
니파야자 꽃대를 채취하고, 깨끗이 세척한 후, 이를 10cm 길이로 절단하였다. 50℃의 열풍으로 건조하여 함수율이 15%가 되도록 하고, 이를 100mesh 정도로 분쇄한 후, 이에 20중량배의 증류수를 가하고, 90~100℃에서 20시간 동안 추출하였다. 그리고 이를 여과한 후, 감압농축기를 이용하여 50℃에서 감압 농축하고, 동결건조하여 분말 상의 추출물을 얻었다.The nipponia palms were picked, cleaned and then cut into 10 cm lengths. Dried by hot air at 50 캜 to have a water content of 15%, and pulverized to about 100 mesh. 20 wt. Parts of distilled water was added thereto, and the mixture was extracted at 90 to 100 캜 for 20 hours. After filtration, the filtrate was concentrated under reduced pressure at 50 ° C using a vacuum concentrator and lyophilized to obtain a powdery extract.
(실시예 2: 니파야자 꽃대 분말의 제조)(Example 2: preparation of nipah palaceae powder)
니파야자 꽃대를 채취하고, 깨끗이 세척한 후, 이를 10cm 길이로 절단하였다. 그리고 이를 100℃의 끓는 물에 30분간 데치고, 데친 꽃대를 찬물에 담가 식혔다. 다음으로, 상기 데친 꽃대를 직경 0.8cm 정도로 결대로 찢어준 후, 탈수기에 넣어 탈수하였다. 그리고 이를 60℃의 열풍건조기를 이용하여 수분함량이 7%가 되도록 건조하였다. 다음으로, 건조된 꽃대를 분쇄기로 1차 및 2차 분쇄하여 약 500mesh의 입도를 갖는 분말을 제조하였다. The nipponia palms were picked, cleaned and then cut into 10 cm lengths. Then, it was boiled in boiling water at 100 ° C for 30 minutes, and the vestibule was soaked in cold water and cooled. Next, the corolla was torn to a diameter of about 0.8 cm, and dehydrated in a dehydrator. Then, it was dried at a moisture content of 7% by using a hot-air dryer at 60 ° C. Next, the dried peduncle was first and second pulverized with a pulverizer to prepare a powder having a particle size of about 500 mesh.
(실시예 3: 간장 및 된장의 제조)(Example 3: preparation of soy sauce and miso)
물 1kg, 실시예 1의 니파야자 꽃대의 추출물 300g을 혼합하고, 이에 천일염을 첨가하여 염도가 17%(w/w)인 염수를 제조하였다. 그리고 제조된 염수 1kg에 메주 180g을 투입하고, 이에 다시 실시예 2의 니파야자 꽃대 분말 100g을 투입하였다. 그리고 이를 정치하여 20℃에서 1년간 숙성시켰다. 그리고 상기 숙성된 숙성물로부터 고형상과 액상을 분리하여 간장과 된장을 얻었다.1 kg of water and 300 g of the extract of the nifa palm phlox of Example 1 were mixed and then a salt of 17% (w / w) was prepared by adding sun-salt. Then, 180 g of meju was added to 1 kg of the prepared brine, and 100 g of the nifa palm phalaenopsis powder of Example 2 was added thereto. The mixture was allowed to stand at 20 ° C for one year. Soy sauce and miso were obtained by separating solid form and liquid form from the aged aged material.
(실시예 4: 고추장의 제조)(Example 4: Preparation of Kochujang)
물 2kg, 엿기름 200g, 실시예 1의 니파야자 꽃대 추출물 200g을 혼합하고, 50℃에서 2시간 동안 당화시켜 상등액을 수득하였다. 그리고 찹쌀가루 200g에 물 600g을 가하고, 100℃에서 20분간 가열하여 풀을 제조하였다.2 kg of water, 200 g of malt, 200 g of the Isolate of the nifa palm of Example 1 were mixed and saccharified at 50 DEG C for 2 hours to obtain a supernatant. Then, 600 g of water was added to 200 g of glutinous rice flour, and the mixture was heated at 100 캜 for 20 minutes to prepare a paste.
다음으로, 상기 수득한 상등액과 상기 제조된 풀을 1:1 중량비로 혼합하고, 60℃에서 4시간 당화시킨 후, 그 부피가 1/2이 될 정도로 농축시켰다. 그리고 상기 농축액 500g에 고추가루 40g, 메주가루 40g, 소금 6g, 실시예 2의 니파야자 꽃대 분말 40g을 혼합하고, 18℃에서 2개월간 숙성시켰다.Next, the supernatant obtained above and the above-prepared paste were mixed at a weight ratio of 1: 1, saccharified at 60 ° C for 4 hours, and then concentrated to a volume of 1/2. 40 g of chili powder, 40 g of meju powder, 6 g of salt and 40 g of the nifa palm phalaenopsis powder of Example 2 were mixed with 500 g of the concentrate and aged at 18 캜 for 2 months.
(실시예 5: 쌈장의 제조)(Example 5: preparation of sesame seeds)
고추장 500g, 된장 500g, 실시예 1의 니파야자 꽃대 추출물 25g, 실시예 2의 니파야자 꽃대 분말 150g을 혼합하였다. 이때, 고추장과 된장은 시판상품을 사용하였다.500 g of soybean paste, 500 g of miso, 500 g of soybean paste, 25 g of the extract of Example 1, and 150 g of the nipahaya phalaenopsis powder of Example 2 were mixed. At this time, Korean pepper paste and miso were commercial products.
(비교예 1)(Comparative Example 1)
실시예 3과 동일하게 실시하되, 니파야자 꽃대 추출물과 분말은 사용하지 않았다.The same procedure as in Example 3 was carried out, except that the extracts of Nephala palmaris and powder were not used.
(비교예 2)(Comparative Example 2)
실시예 4와 동일하게 실시하되, 니파야자 꽃대 추출물과 분말은 사용하지 않았다.The same procedure as in Example 4 was carried out except that the extracts and powders of Nephala palm are not used.
(비교예 3)(Comparative Example 3)
실시예 5와 동일하게 실시하되, 니파야자 꽃대 추출물과 분말은 사용하지 않았다.The same procedure as in Example 5 was carried out, except that the extracts of Nihon palmensis and powder were not used.
(시험예 1)(Test Example 1)
니파야자 꽃대 추출물의 항산화력 평가.Evaluation of Antioxidative Capacity of Extracts of.
실시예 1과 같이 제조한 니파야자 꽃대 추출물의 항산화력을 측정하였다.The antioxidative activity of the extracts of Nephala palmetae prepared in Example 1 was measured.
상기 추출물은 냉동보관하면서 실험에 사용하였다.The extract was used in the experiment while being kept frozen.
(1) DPPH radical 소거능 측정(1) Measurement of DPPH radical scavenging ability
항산화 활성 측정은 DDPH(1,1-diphenyl-2-picryhydrazyl, Sigma-Aldrich Co.)를 이용하여 시료의 라디칼 소거효과를 보는 Biois법을 활용하였다. 각 추출물을 농도별 0.025~10mg/ml로 제조한 시료 용액 1ml에 0.2mM DPPH 1ml를 넣고, vortex mixer를 사용하여 혼합하고 암실에서 30분간 반응시킨 후 517nm에서 흡광도를 측정하였다. 그리고 아래의 식을 이용하여 free radical scavenging activity 곡선을 작성한 후, IC50을 산출하였으며, 양성대조군으로 ascorbic acid를 사용하였다. To determine the antioxidant activity, the Biois method was used to examine the radical scavenging effect of DDPH (1,1-diphenyl-2-picryhydrazyl, Sigma-Aldrich Co.). 1 ml of 0.2 mM DPPH was added to 1 ml of the sample solution prepared from 0.025 to 10 mg / ml of each extract, mixed using a vortex mixer, reacted for 30 minutes in a dark room, and absorbance was measured at 517 nm. Then, free radical scavenging activity curves were generated using the following equation, and IC 50 was calculated. As a positive control, ascorbic acid was used.
Radical scavenging activity(%)={(control Absorbance - Sample Absorbance)/control Absorbance}×100Radical scavenging activity (%) = {(control Absorbance - Sample Absorbance) / control Absorbance} × 100
(2) ABTS radical 소거능 측정(2) Measurement of ABTS radical scavenging ability
ABTS radical 소거능 측정은 Pellegrin 등의 방법으로 측정하였다. 7mM ABTS(2,2'-azinobis(3-ethylenbenzothiazoline-6-sulfonate), Sigma-Aldrich Co.) 5ml와 140mM K2S2O8(Sigma-Aldrich Co.) 88㎕를 잘 섞어 16시간 이상 암소에 방치시켰다. ABTS radical scavenging activity was measured by Pellegrin et al. 5 ml of 7 mM ABTS (2,2'-azinobis (3-ethylenbenzothiazoline-6-sulfonate), Sigma-Aldrich Co.) and 88 μl of 140 mM K 2 S 2 O 8 (Sigma-Aldrich Co.) .
이를 absolute ethanol과 1:88 비율로 섞어 734nm에서 대조구의 흡광도 값이 0.7±0.002가 되도록 조절한 ABTS solution을 만들었다. 시료 용액 50㎕에 ABTS solution 1ml를 넣어 혼합하고, 2.5분간 암실에서 방치한 후, 734nm에서 흡광도를 측정하였다.The mixture was mixed with absolute ethanol at a ratio of 1:88, and the ABTS solution was prepared so that the absorbance of the control was adjusted to 0.7 ± 0.002 at 734 nm. 1 ml of ABTS solution was added to 50 μl of the sample solution, and the mixture was allowed to stand in a dark room for 2.5 minutes, and the absorbance was measured at 734 nm.
그 결과는 하기 표 3 및 표 4에 나타내었다.The results are shown in Tables 3 and 4 below.
(mg/ml)Concentration
(mg / ml)
(mg.ml)IC 50
(mg.ml)
0.125
0.0625
0.03125
0.156250.025
0.125
0.0625
0.03125
0.15625
51.52±1.09
26.74±1.51
13.93±2.09
7.82±0.3785.83 + - 0.22
51.52 ± 1.09
26.74 1.51
13.93 ± 2.09
7.82 + - 0.37
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
44.73±0.74
36.62±0.49
30.87±0.41
28.79±0.4160.70 + - 0.47
44.73 + - 0.74
36.62 ± 0.49
30.87 + - 0.41
28.79 + - 0.41
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
37.41±0.41
32.67±0.43
30.27±0.14
28.98±0.4344.89 + - 0.41
37.41 + - 0.41
32.67 + - 0.43
30.27 + - 0.14
28.98 + - 0.43
IC50 값은 추출물을 첨가하지 않은 대조군의 값을 50% 감소시키는 추출물의 농도를 나타낸 것으로, IC50 값이 작을수록 항산화 활성이 높은 것을 뜻한다. 따라서 상기 표 3에서와 같이, DPPH radical scavenging activity 측정 결과에 따르면 니파야자 꽃대의 항산화능은 블루베리에 비하여 약 40배 높고, 홍삼에 비해 약 75배 높음을 알 수 있었다.The IC 50 value represents the concentration of the extract which reduces the value of the control without the extract by 50%. The smaller the IC 50 value, the higher the antioxidant activity. Therefore, as shown in Table 3, according to DPPH radical scavenging activity measurement results, the antioxidant activity of the Nypha palm phlox was about 40 times higher than that of blueberry and about 75 times higher than that of red ginseng.
(mg/ml)Concentration
(mg / ml)
(mg.ml)IC 50
(mg.ml)
0.125
0.0625
0.03125
0.156250.025
0.125
0.0625
0.03125
0.15625
23.28±0.99
11.54±0.94
5.87±0.27
2.50±0.5644.44 + - 0.42
23.28 ± 0.99
11.54 + - 0.94
5.87 ± 0.27
2.50 ± 0.56
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
10.05±0.48
5.40±0.17
3.12±0.15
2.08±0.5917.68 ± 0.51
10.05 + 0.48
5.40 ± 0.17
3.12 ± 0.15
2.08 ± 0.59
0.5
0.25
0.125
0.0625One
0.5
0.25
0.125
0.0625
6.09±0.51
3.02±0.31
1.58±0.56
0.25±0.3111.89 + - 0.54
6.09 ± 0.51
3.02 ± 0.31
1.58 ± 0.56
0.25 0.31
상기 표 4에서와 같이, ABTS radical scavenging activity 측정 결과에 따르면, 니파야자 꽃대의 항산화능은 블루베리에 비하여 약 42배 높고, 홍삼에 비해 약 59배 높음을 알 수 있었다.As shown in Table 4, according to the result of ABTS radical scavenging activity measurement, the antioxidant activity of Nypha palm phlox was about 42 times higher than that of blueberry and about 59 times higher than that of red ginseng.
(시험예 2)(Test Example 2)
염도 측정Salinity measurement
상기 실시예 3, 4에서 제조된 간장, 된장, 고추장의 염도를 염도측정기를 이용하여 측정하고, 대조군으로 일반 상용제품을 동일 방법으로 측정하여 비교하였다. The salinity of the soy sauce, doenjang, and kochujang prepared in Examples 3 and 4 was measured using a salinity measuring device, and the general commercial products were measured and compared in the same manner as the control group.
그 결과는 하기 표 5에 나타내었다.The results are shown in Table 5 below.
상기 표 5에서 확인할 수 있는 바와 같이, 본 발명에 따른 간장, 된장, 고추장은 대조군에 비해 그 염도가 확연히 낮음을 확인하였다.As shown in Table 5, it was confirmed that the salinity of soy sauce, doenjang, and kochujang according to the present invention was significantly lower than that of the control group.
(시험예 3)(Test Example 3)
저장성 평가Evaluation of shelf life
상기 실시예 3 내지 5, 비교예 1 내지 3에서 제조된 간장, 된장, 고추장, 쌈장을 제조직 후, 제조 1개월, 제조 1년, 제조 3년의 시간경과에 따라, 맛, 장기보관 안정성, 제품외관변화를 관찰하여, 하기 표 6에 기재하였다.The storage stability of the soy sauce, miso, hot pepper paste, and sesame oil prepared in Examples 3 to 5 and Comparative Examples 1 to 3 over time, after 1 month of manufacture, 1 year of manufacture and 3 years of production, Changes in appearance of the product were observed and are shown in Table 6 below.
시간경과에 따라, 초기 맛과 변화없이 유지되면 ○, 시큼하거나 떨떠름한 식미감이 관찰되면 △, 먹기 불편할 정도로 풍취와 씹히는 식감이 떨어지면 ×로 표기하였다. ∘, when the taste was maintained without changes in the initial taste and with time, △ when the sour or the rough taste was observed, and ⊚ when the taste was not enough to eat.
장기보관 안정성은 초기 제품의 맑음 정도가 시간경과에 따라, 변화없이 유지되면 ○, 맑지 않고 다소 탁함이 관찰되면 △, 용기 주변에 곰팡이가 관찰되면 ×로 표기하였다. The stability of long-term storage was evaluated as ○ when the degree of clearness of the initial product was maintained without change, ○ when it was not clear, when there was slight turbidity, and when the mold was observed around the container.
제품외관은 초기 제품의 염도 및 당도 측정결과, 변화없이 유지되면 ○, ±5 이내의 측정치 변화가 관찰되면 △, ±5 이내의 측정치 변화가 관찰되면 ×로 표기하였다.The appearance of the product is indicated by ∘ when the salinity and sugar content of the initial product are maintained without change, △ when the change of the measured value within ± 5 is observed, and by × when the change of the measured value within ± 5 is observed.
The miso of Example 3
상기 표 6에서 확인할 수 있는 바와 같이, 본 발명에 따른 간장, 된장, 고추장, 쌈장은 저염화했음에도 보존성이 우수하나, 비교예 1, 2, 3은 저염화에 따라 제조 1년 후부터는 보존성이 좋지 못함을 확인하였다.As can be seen from Table 6, the soy sauce, miso, pepper paste, and sesame seed according to the present invention are excellent in preservability even when they are low in chloride, but Comparative Examples 1, 2 and 3 have poor preservability after one year of production due to low chlorination Respectively.
(시험예 4)(Test Example 4)
관능성 평가.Sensory evaluation.
상기 실시예 3 내지 실시예 5 및 비교예 1 내지 비교예 3으로부터 제조된 간장, 된장, 고추장 및 쌈장에 대하여 관능성 테스트를 실시하였다. 상기 평가는 잘 훈련받은 관능요원 15명을 대상으로 향, 색, 맛 및 전체적인 기호도를 평가하게 한 후, 평균값을 하기 표 7에 나타내었다.The soy sauce, doenjang, kochujang, and sesame jam prepared from Examples 3 to 5 and Comparative Examples 1 to 3 were subjected to a sensory test. The above evaluation was conducted to evaluate the fragrance, color, taste and overall acceptability of 15 well-trained sensory agents, and the average values are shown in Table 7 below.
- 5점: 매우 우수하다- 5 points: Very good
- 4점: 우수하다- 4 points: Excellent
- 3점: 보통- 3 points: Normal
- 2점: 좋지 않다- 2 points: Not good
- 1점: 매우 좋지 않다- 1 point: Not very good
상기 표 7에서 알 수 있는 바와 같이, 니파야자의 꽃대 분말 및 추출물을 포함하는 실시예 3 내지 5의 장류는 니파야자의 꽃대 추출물을 포함하지 않는 비교예 1 내지 3에 비해 향, 맛 및 전체적인 기호도가 우수함을 확인하였다. As can be seen in Table 7, the intestines of Examples 3 to 5 containing the phalaenopsis powder and extract of NiPhaya had a higher flavor, taste and overall acceptability than Comparative Examples 1 to 3, Respectively.
이상, 본 발명을 바람직한 실시예를 사용하여 상세히 설명하였으나, 본 발명의 범위는 특정 실시예에 한정되는 것은 아니며, 첨부된 특허청구범위에 의하여 해석되어야 할 것이다. 또한, 이 기술분야에서 통상의 지식을 습득한 자라면, 본 발명의 범위에서 벗어나지 않으면서도 많은 수정과 변형이 가능함을 이해하여야 할 것이다.While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the scope of the present invention is not limited to the disclosed exemplary embodiments. It will also be appreciated that many modifications and variations will be apparent to those skilled in the art without departing from the scope of the present invention.
Claims (7)
니파야자 꽃대의 추출물, 니파야자 꽃대 분말 또는 니파야자 꽃대의 추출물과 분말 모두를 첨가하여 제조하는 것을 특징으로 하는 니파야자 꽃대를 이용한 기능성 장류의 제조방법.
Soy sauce, soybean paste, soybean paste, hot pepper paste, and sesame seed,
A method for the production of a functional soybean using a nifa palm phlox, wherein the extract is produced by adding both an extract of the nifa palm phloem, a nifa palm phalaenopsis powder or an extract and powder of the nifa palm phalaenopsis.
물 100중량부, 니파야자 꽃대의 추출물 20~40중량부를 포함하며, 염도가 16~18%(w/w)인 염수를 제조하는 단계와,
상기 제조된 염수에 메주를 투입하는 단계와,
상기 메주가 포함된 염수 100중량부에 니파야자 꽃대 분말 1~10중량부를 투입하는 단계와,
상기 니파야자 꽃대 분말이 투입된 혼합물을 정치하여 1~2년간 숙성시키는 단계와,
상기 숙성된 숙성물로부터 간장과 된장을 얻는 단계를 포함하는 것을 특징으로 하는 니파야자 꽃대를 이용한 기능성 장류의 제조방법.
The method according to claim 1,
100 parts by weight of water and 20 to 40 parts by weight of an extract of the nifa palm phlox, wherein the salt water has a salinity of 16 to 18% (w / w)
Introducing meju into the prepared brine;
Adding 1 to 10 parts by weight of a Nephala palm leaf powder to 100 parts by weight of brine containing meju,
Aging the mixture in which the above-mentioned Nipahaya phalaenopsis powder is placed for 1 to 2 years,
And a step of obtaining soy sauce and doenjang from the aged mature product.
물 100중량부, 엿기름 5~10중량부, 니파야자 꽃대 추출물 5~10중량부를 혼합한 후, 1~3시간 동안 당화시켜 상등액을 수득하는 단계와,
찹쌀 또는 멥쌀가루에 물을 투입하고 가열하여 풀을 제조하는 단계와,
상기 수득된 상등액에 상기 제조된 풀을 투입하여 3~7시간 당화시키는 단계와,
상기 당화된 당화액 100중량부에 고추가루 5~10중량부, 메주가루 5~10중량부, 소금 1~2 중량부 및 니파야자 꽃대 분말 1~10중량부를 혼합하는 단계와,
상기 혼합된 혼합물을 15~20℃에서 1~2개월간 숙성시켜 고추장을 얻는 단계를 포함하는 것을 특징으로 하는 니파야자 꽃대를 이용한 기능성 장류의 제조방법.
The method according to claim 1,
Mixing 5 to 10 parts by weight of water, 5 to 10 parts by weight of maltose and 5 to 10 parts by weight of an extract of Nephala palmetto and then saccharifying the mixture for 1 to 3 hours to obtain a supernatant;
Adding water to glutinous rice or rice flour and heating to prepare a paste,
Adding the prepared paste to the obtained supernatant and saccharifying it for 3 to 7 hours,
Mixing 5 to 10 parts by weight of red pepper powder, 5 to 10 parts by weight of meju powder, 1 to 2 parts by weight of salt, and 1 to 10 parts by weight of nifalaxy powder with 100 parts by weight of the saccharified saccharified liquid,
And aging the mixed mixture at 15 to 20 占 폚 for 1 to 2 months to obtain kochujang.
고추장 100중량부에 된장 50~100중량부, 니파야자 꽃대 추출물 5~10중량부, 니파야자 꽃대 분말 20~50중량부를 혼합하는 단계를 포함하는 것을 특징으로 하는 니파야자 꽃대를 이용한 기능성 장류의 제조방법.
The method according to claim 1,
Mixing 50 to 100 parts by weight of miso with 5 to 10 parts by weight of an extract of Nipponia cunea, and 20 to 50 parts by weight of a nipponia coconut palm flower powder in 100 parts by weight of a Kochujang. Way.
상기 니파야자 꽃대 추출물은,
니파야자의 꽃대를 물을 용매로 하여 열수추출 또는 초음파추출한 것이고,
상기 니파야자 꽃대 분말은, 니파야자 꽃대를 절단하여 물에 데친 후, 탈수, 건조 및 분쇄한 것임을 특징으로 하는 니파야자 꽃대를 이용한 기능성 장류의 제조방법.
5. The method according to any one of claims 1 to 4,
The above-mentioned < RTI ID = 0.0 >
The phloem of Nipaya is water-extracted or ultrasonically extracted with water as a solvent,
The method for producing a functional soybean flour according to any one of claims 1 to 3, wherein the nifa palm phalaenopsis powder is obtained by cutting a nifa palm phlox, padding it in water, and then dehydrating, drying and grinding it.
상기 니파야자 꽃대 분말의 혼합 시,
홍화씨 분말 1~3중량부를 더 혼합하는 것을 특징으로 하는 니파야자 꽃대를 이용한 기능성 장류의 제조방법.
5. The method according to any one of claims 2 to 4,
Upon mixing of the above-mentioned < RTI ID = 0.0 >
And 1 to 3 parts by weight of a safflower seed powder is further mixed.
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KR20050010914A (en) | 2002-06-13 | 2005-01-28 | 가부시키가이샤 호오도 | Antibacterial agent and antibacterial composition |
KR20050009505A (en) * | 2003-07-16 | 2005-01-25 | 우창근 | Soybean paste and soy sauce manufaturing process |
KR101105165B1 (en) | 2011-04-14 | 2012-01-17 | 최원석 | Method for preparing korean hot pepper paste using jerusalem artichoke |
KR20150141098A (en) * | 2014-06-09 | 2015-12-17 | 권호성 | Method for manufacture of allium hookeri composition comprising allium hookeri powder or allium hookeri extracts and allium hookeri composition and method for manufacture of functional soybean paste using allium hookeri composition |
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