KR20180036167A - Composition for increasing muscular strength and muscle mass comprising bean juice-peptides derived from germinated bean treated with green mandarin water - Google Patents

Abstract

The present specification provides a composition comprising an extract from soybeans as an effective ingredient for enhancing muscular strength, increasing muscle mass, alleviating a muscle disease and a metabolic syndrome, and improving stamina, wherein the soybeans are treated with a liquid obtained from an unripened fruit of a plant of the genus Citrus or with a processed product of the liquid, so that the composition can contribute to the promotion of related consumer welfare and industrial development.

Description

Technical Field [0001] The present invention relates to a composition for improving muscle strength and muscular mass comprising a juice peptide derived from germinated soybean, which is treated with a citrus jujube. TECHNICAL FIELD [0001] The present invention relates to a composition for improving muscle strength and muscle mass,

The present invention relates to a composition for enhancing muscle strength or muscle mass comprising, as an active ingredient, a liquid obtained from immature fruit of a plant of the genus Citrus (Citrus) or an extract of soybeans treated with the composition.

In the case of overweight or obese people or people with normal body weight but high body fat, metabolic syndrome such as diabetes and hypertension can be prevented and treated by increasing muscle mass and decreasing body fat. Basal metabolism energy is increased due to increase of muscle mass, Efficient diet is possible. In order to increase muscle mass, exercise, diet and ergogenic aids have been used, and studies related to functional foods for improving athletic performance have been actively conducted, both east and west. On the other hand, supplements containing chemical compounds such as steroids, caffeine, sodium hydrogencarbonate and sodium citrate may significantly increase exercise performance when taken over a certain amount, but such drugs may lead to fatal health problems Side effects may follow. In recent years, studies have been actively carried out to develop functional foods using natural substances having high safety, while having few side effects such as plant extracts, rather than chemical synthetic products.

Soybean has been consumed as a traditional food material for a long time in various regions such as Asia, and soybean itself or a variety of processed products thereof have been developed. However, the technology for improving the quality and functional value of the main nutrients of soybean .

Japanese Laid-Open Patent No. 1994-172119

The present invention has a purpose to provide a composition for improving muscular strength or muscle mass, a composition for improving metabolic syndrome, etc., by treating soybean through a liquid derived from an immature fruit of the genus Citrus, a natural substance.

In order to solve the above problems, the present invention provides, in one aspect, a composition for enhancing muscle strength or muscle mass comprising, as an active ingredient, a liquid obtained from immature fruit of a plant of the genus Citrus or an extract of soybeans treated with the processed product do.

In another aspect, the present invention relates to a composition for preventing or treating muscle diseases caused by muscle wasting, damage or degradation, which comprises an extract of soybeans treated with a liquid obtained from immature fruit of a plant of the genus Citrus, .

In another aspect, the present invention provides a composition for the prevention or treatment of metabolic syndrome comprising, as an active ingredient, a liquid obtained from immature fruit of a plant belonging to the genus Citrus, or an extract of soybeans treated with the processed product.

In another aspect, the present invention provides a composition for enhancing physical fitness comprising an extract of soybeans treated with a liquid obtained from immature fruit of a plant belonging to the genus Citrus or a processed product thereof as an active ingredient.

According to one aspect of the present invention, by using the liquid obtained from the immature fruit of the plant of the genus Citrus, or the soybean extract treated with the processed product, it is possible to strengthen the muscle, improve the muscle mass, improve the metabolic syndrome, , Which will contribute to the improvement of care and welfare of related consumers and patients, and will help to develop the relevant industries.

FIG. 1 shows that the extract according to Example 4 improves protein content in muscle cells in a concentration-dependent manner.
Fig. 2 shows that the extract according to Example 4 promotes phosphorylation of S6K. Fig.
Figure 3 is a schematic representation of protein production enhanced and cell growth enhanced by the mTOR pathway.
4 is a graph showing that the extract according to Example 4 affects an index related to the metabolic syndrome.
Figure 5 shows that the extract according to Example 4 contributes to the increase in muscle mass.
Fig. 6 shows that the extract according to Example 4 causes decrease in body fat mass and increase in skeletal muscle mass.
Figure 7 shows that the extract according to Example 4 had an effect on the improvement of muscle function and flexibility.
Figure 8 shows the effect of the extract according to Example 4 on flexibility, cardiopulmonary function, muscle strength function and body shape.
9 shows the effect of the extract according to Example 4 on waist circumference, blood sugar, triglyceride, and total cholesterol content.
10 shows the effect of the extract according to Example 4 on improvement of fatigue and improvement of condition.

Hereinafter, the present invention will be described in detail.

As used herein, the term " liquid obtained from immature fruit of a plant of the genus Citrus " includes all liquids derived from immature fruits of a plant belonging to the genus Citrus. This includes liquids that have been sourced from at least one of the fruit and the shell without processing or processing the unmatured fruit of the plant belonging to the genus Citrus. The liquid obtained by subjecting the juice to a variety of processing and processing steps followed by distillation, and a liquid obtained by adding purified water or ionized water to the obtained liquid.

The term " green mandarin water (GMW) " as used herein means a solution obtained by juicing at least one of immature citrus fruits and shells, a solution obtained by processing or treating the solution and then distilling the solution, And diluted with purified water or ionized water.

In the present specification, the term " workpiece " means any material obtained by processing a material as a raw material by a conventional method, and specifically includes an extract, a heated product, a dried product, a fermented product, a hydrolyzed product, , But is not limited thereto.

As used herein, the term "dry bean" refers to beans that have been reduced in moisture to 10% (w / w) or less in soy bean by drying. The above drying can be carried out by any ordinary drying method, but freeze drying is preferred.

As used herein, the term "extract" refers to a substance obtained by extracting a component contained therein from a natural product, regardless of the method of extraction or the kind of the component. For example, those obtained through one or more of pulverization and juice without a solvent, those obtained by extracting components dissolved in a solvent using water or an organic solvent, those obtained by extracting only specific components such as amino acids, and the like And the like.

In one aspect, the present invention may be a composition for enhancing muscle strength or muscle mass comprising, as an active ingredient, a liquid obtained from immature fruit of a plant belonging to the genus Citrus, or an extract extracted from soybeans treated with the composition.

According to one embodiment, the muscle strengthening or muscle mass enhancement may be due to an increase in the amount of protein in the muscle cells.

According to another embodiment, the muscle strengthening or muscle mass enhancement may be due to activation of the mammalian target of rapamycin (mTOR) pathway in muscle cells.

According to another embodiment, the muscle strengthening or muscle mass enhancement may be due to phosphorylation of the S6K (ribosomal protein S6 kinase) protein signal in muscle cells.

In another aspect, the present invention relates to a method for preventing muscle wasting, muscle damage or muscle degeneration caused by muscle wasting or muscle degeneration, which comprises an extract obtained from an immature fruit of a plant belonging to the genus Citrus, Or a therapeutic composition.

According to one embodiment, muscle wasting or muscle degeneration may occur due to genetic factors, acquired factors, aging, etc., and muscle wasting may result in gradual loss of muscle mass, weakness and degeneration of muscles, particularly skeletal muscle or vascular and cardiac muscle . ≪ / RTI > Examples of diseases associated therewith may include atony, muscular atrophy, muscular dystrophy, muscle degeneration, myasthenia, cachexia and sacopenia.

In another aspect, the present invention may be a composition for preventing or treating metabolic syndrome comprising an extract obtained from immature fruit of a plant belonging to the genus Citrus or an extract extracted from soybeans treated with the composition.

According to one embodiment, the prevention or treatment of the metabolic syndrome may be caused by one or more selected from the group consisting of weight loss, improvement of abdominal obesity, reduction of body fat, reduction of blood sugar, and improvement of body shape.

In another aspect, the present invention may be a composition for enhancing physical fitness comprising, as an active ingredient, a liquid obtained from immature fruit of a plant belonging to the genus Citrus, or an extract extracted from soybeans treated with the processed product.

According to one embodiment, the physical fitness may be one or more selected from the group consisting of cardiopulmonary function enhancement, flexibility enhancement, and fatigue relief.

According to another embodiment, the liquid may be a liquid obtained by distilling and liquefying a liquid juice from immature fruit.

According to another embodiment, the liquid may be a liquid in which the immature fruit enzyme is inactivated. According to one embodiment, the juice of the immature fruit may be one in which the immature fruit enzyme is inactivated through at least one of heat treatment and high-pressure treatment. In addition, immature fruits of the genus Citrus can be squeezed and pressurized to obtain water and other solid and fragrant components in the fruit, and then heat-treated or high-pressure treated to inactivate the enzymes. In another step of separating the citrus juice of one embodiment of the present invention, the citrus peel can be juiced and heat treated to inactivate the enzyme.

According to one embodiment, any of the methods known to those skilled in the art can be used to distill and liquefy the juice. For example, in order to distill the juice, a heat treatment can be performed. The heat treatment may be performed at about 100 to 121 占 폚 under pressure, about 80 to 100 占 폚 at normal pressure, or 40 to 80 占 폚 under reduced pressure. Further, according to one embodiment of the present invention, it is further possible to separate and remove undesirable components by centrifugation, membrane separation or distillation separation in the step before or after the distillation of the juice.

According to another embodiment, the heat treatment may be carried out at a temperature of 50 to 150 ° C. When the temperature range is within the above range, the enzyme contained in the immature fruit of the citrus-like plant can be inactivated by the heat treatment. In addition, it is possible to prevent contamination or denaturation by inhibiting or killing the growth of microorganism bacteria through the heat treatment.

According to another embodiment, the juice may be at least one selected from the group consisting of a gear type juice, a press type juice, a crushed juice and an enzymatically degraded juice using a pressing effect.

According to another embodiment, the liquid may be further subjected to a step of mixing and distilling an organic solvent.

According to another embodiment, the liquid or a workpiece thereof is composed of linalool, terpinene-4-ol, terpineol and citronellol. , ≪ / RTI >

Further, according to an embodiment, the treatment may be to immerse with a liquid obtained from immature fruit of a citrus plant or a work thereof. According to one embodiment, the immersion may be carried out for 5 to 24 hours. According to another embodiment, a mixture of a liquid (or a workpiece) obtained from immature fruit of a certain amount of a Citrus genus plant and a green tea extract containing a certain amount of catechin may be used in the immersion. According to one embodiment, the content is 0.1 wt% or more, 0.5 wt% or more, 1 wt% or more, 2 wt% or more, 3 wt% or more, 4 wt% or more, 5 wt% or more, 6 wt% or more, Or less, 15 weight% or less, 12 weight% or less, 10 weight% or less, 8 weight% or less, 6 weight% or less, Or less, 5 wt% or less, 4 wt% or less, 3 wt% or less, 2 wt% or less, 1 wt% or less, 0.5 wt% or less, or 0.1 wt% or less. The above content may preferably be 2 to 5% by weight. According to another embodiment, the catechin content in the green tea extract is at least 1 wt%, at least 3 wt%, at least 5 wt%, at least 7 wt%, at least 9 wt%, at least 10 wt%, at least 12 wt% At least 20 wt%, at least 23 wt%, at least 25 wt%, at least 28 wt%, at least 30 wt%, at least 32 wt%, at least 35 wt%, at least 37 wt% %, At least 45 wt.%, At least 50 wt.%, At least 52 wt.%, At least 55 wt.%, Or at least 60 wt.%, By weight, up to 50% by weight, up to 45% by weight, up to 42% by weight, up to 40% by weight, up to 37% by weight, up to 35% by weight, up to 30% Up to 23 wt%, up to 20 wt%, up to 18 wt%, up to 15 wt%, up to 12 wt%, up to 10 wt% up to 9 wt% up to 7 wt% up to up to 5 wt% % ≪ / RTI > The catechin content in the green tea extract may preferably be 35% by weight.

According to another embodiment, the Citrus plant is selected from the group consisting of Citrus maxima , Citrus medica , Citrus reticulate , Citrus trifoliate , Citrus japonica , Lime, Citrus fruit, paradise , citrus limon , citrus aurantium , Citrus junos Or Citrus ichangenis , Citrus unshiu , Satsuma mandarin, and Sweet orange ( Citrus sinensis ), preferably citrus.

According to another embodiment, the immature fruit comprises all of the unmatured fruit, and the fruit juice comprises the kumquat.

In one aspect, the lime Australia Raleigh eyes finger limes (Citrus australasica), Australia Raleigh, not round lime (Citrus australis), Australia Raleigh Do not Desert Lime (Citrus glauca), Mount White limes (Citrus garrawayae), Kakadu lime (Citrus gracilis , Citrus inodora , Citrus warburgiana , and Citrus wintersii ).

According to another embodiment, the Citrus plant may be mandarin ( Citrus reticulate ) or satsuma mandarin.

According to one embodiment, the soybeans can be used without limitation if they are germable beans. For example, it may be a varietal for soybean curd, tofu, herb, rice or soybean. Among the varieties for soy sauce and tofu, there are Daifeng, Hojang, Jangwon, Daehang, Sodam, Songhak, Daewon, , Protein, soybean oil, Shinpaldal, Taekwang, Manly, Jangsu, Wuhan, Baekun, Baal, ) And Chang Le (长 葉). Among the herb cultivars, there are three kinds of herbaceous varieties: Shinhwa, Suwon, Anping, Seinam, Daekwon, Sook, Soo, Daewon, Poongsan There are many herbs, Iksan herbs, small white herbs, gwangan, short leaves and galaxies. The rice varieties include celadon, black celery, brown celery, black celery, black celery, and one black culinary variety. In addition, the varieties for soybeans include daol, green, new, black, soya, soybeans, and so on. In another aspect, the soybean is preferably a variety capable of germination and resistant to pests and diseases. Such beans include, for example, myths, wishes, Anping, southwestern, colorful, small lakes, small lakes, calligraphy, ), Poongsan herbs, Iksan herbs, Sobaek herbs, Gwangan, short leaves and galaxies. However, in this specification, the soybeans for germination are not limited to the kinds of the soybean.

Examples of scientific names for the soybean include Lupinus angustifolius L., Phaseolus vulgaris, Glycine max (L.) Merr., Cyamopsis tetragonoloba (L.) Taub , Phaseolus radiatus L. var. aurea , Phaseolus vulgaris L., Canavalia gladiatia , Glycine soja Sieb . & Zucc ., Glycine ussuriensis Regel & Maack , Arachis hypogaea L., Lens culinaris Medik . , Phaseolus lunatus L., Amphicarpaea bracteata , Phaseolus multiflous Wild., Cajanus cajan (L.) Millsp . , Vicia angustifolia L. there is. segetilis , Amphicarpaea edgeworthii Benth ., Rhynchosia nulubilis , Cicer arietinum L., Apios Americana, Vicia faba L., Dolichos lablab L., Ceratonia siliqua (L.) Taub ., Dipteryx odorata ( Aub L. ) Willd , Phaseolus There is multiflorus . albus Bailey , and the like, but are not limited thereto. Also beans with the same scientific name can be classified into various kinds of beans according to improvement, hybridization, color, size and variety.

The soybean may be the soybean germinated after the treatment, and the soybean may be the soybean. According to one embodiment, the germination may be carried out for 12 to 48 hours. According to another embodiment, the germination may be at least one of germination and cancer germination.

According to an aspect of the present invention, there may be no process of boiling the soybeans after the germination. Conventional methods for obtaining soybean extract are accompanied by a process of boiling soybeans, but in one aspect of the present invention, such boiling process may be excluded to minimize nutrient destruction.

According to another embodiment, the extraction may comprise one or more of crushing and juice. For example, the juice may be at least one selected from the group consisting of a gear type juice, a press type juice, a crushed juice, and an enzymatically degraded juice, and in particular, by a Korean traditional method of crushing a bean with a millstone and then using a cloth It can be a juice.

According to another embodiment, said extraction may be extraction with water or a C1 to C5 alcohol.

According to another embodiment, the C1 to C5 alcohol may be ethanol.

According to another embodiment, the extraction may further include an enzymatic fermentation or hydrolysis. The fermentation or hydrolysis by the enzyme is preferably performed after the juice, but is not limited thereto. The enzyme is preferably a protease derived from a strain belonging to the genus Bacillus, but is not limited thereto. Such enzymes include Saccharomyces sp . , Bacillus sp . , Lactobacillus sp. And Leuconostoc sp. mesenteroides sp . ) May be an enzyme derived from the strain, preferably a Saccharomyces as MY process three Levy jiae (Saccharomyces cerevisiae) is selected from, Lactobacillus Kasei (Lactobacillus casei), Bacillus subtilis (Bacillus subtlis), Lactobacillus non-vitreous ( Lactobacillus bulgarius ) and Leuconostoc mesenteroides ( Leuconostoc mesenteroides ). < / RTI >

According to another embodiment, the fermentation or hydrolysis may be performed at a temperature above 20 degrees Celsius, above 25 degrees Celsius, above 30 degrees Celsius, above 40 degrees Celsius, above 50 degrees Celsius, above 55 degrees Celsius, above 60 degrees Celsius, It may be carried out by more than Mr. Do. In addition, it shall be conducted at 67 degrees or less, 65 degrees or less, 60 degrees or less, 55 degrees or less, 50 degrees or less, 45 degrees or less, 40 degrees or less, 30 degrees or less, Lt; / RTI > In another embodiment, the fermentation or hydrolysis time is at least 5 hours, at least 7 hours, at least 9 hours, at least 10 hours, at least 12 hours, at least 14 hours, at least 15 hours, at least 16 hours, at least 18 hours, 20 hours or more, or 24 hours or more. It may be 25 hours or less, 24 hours or less, 20 hours or less, 18 hours or less, 16 hours or less, 14 hours or less, 12 hours or less, 10 hours or less, 9 hours or less, 8 hours or less or 7 hours or less.

According to another embodiment, the composition may further comprise at least one selected from the group consisting of a mixture of L-carnitine and an extract obtained by extracting a soybean embryo with hot water, leucine, phenylalanine, and methionine. According to one embodiment, a mixture of the hot-water extract of the soybean embryo ("soybean embryo hot-water extract") and L-carnitine can be a commonly known mixed composition commonly known as "APIC" It may be recognized as an orthopedic health functional food raw material. The mixing ratio of the soybean embryo hydrothermal extract to L-carnitine may be 4: 3 by weight. According to one embodiment, the hot-water extract of soybean embryo is extracted with hot water (90 to 100 degrees C for 30 to 60 minutes) after separating the embryo of soybeans, adsorbed to the resin after filtration, and then washed with 60% ethanol After eluting the eluate, it is concentrated (1 to 2 hours), added with a certain amount of cyclodextrin, sterilized at 90 ° C or higher, and then dried. According to another embodiment, the mixing ratio of the soybean embryo hydrothermal extract to L-carnitine may be m: n by weight, wherein m and n may each be 1 to 5.

According to one embodiment, the extract comprises at least 5%, at least 10%, at least 15%, at least 20%, at least 25%, at least 30% At least 35% by weight, at least 40% by weight, at least 45% by weight, at least 48% by weight, at least 50% by weight, at least 55% by weight, at least 60% by weight and at least 65% by weight. Or less, 70 wt% or less, 65 wt% or less, 60 wt% or less, 55 wt% or less, 50 wt% or less, 48 wt% or less, 45 wt% or less, 40 wt% or less, 35 wt% Or less, 25 weight% or less, 20 weight% or less, 15 weight% or less, or 10 weight% or less. Preferably, the content may be 40 to 60% by weight.

According to another embodiment, the extract may be a composition having one or more of the following characteristics.

a) the content of total free amino acids is from 0.4 to 2.5% by weight, based on the total weight of the extract;

b) 0.1 to 1.5% by weight, based on the total weight of the extract, of BCAA;

c) the content of leucine is 0.03 to 0.1% by weight based on the total weight of the extract;

d) 0.01 to 0.5% by weight, based on the total weight of the extract, of valine;

e) the content of isoleucine is from 0.01 to 0.7% by weight, based on the total weight of the extract;

f) 0.03 to 0.15% by weight, based on the total weight of the extract, of GABA;

g) 0.05 to 0.4% by weight, based on the total weight of the extract, of glutamic acid;

h) 0.01 to 0.5% by weight, based on the total weight of the extract, of phenylalanine; And

i) 0.01 to 0.5% by weight, based on the total weight of the extract, of alanine.

According to one embodiment, the total amino acid content may be 0.4 to 2.5% by weight based on the extract. Specifically, the total amino acid content relative to the total weight of the extract is 0.4 wt% or more, 0.8 wt% or more, 1.0 wt% or more, 1.2 wt% or more, 1.4 wt% or more, 1.6 wt% or more, 1.64 wt% or more, 1.8 wt% , Not more than 2.0 wt%, not more than 2.0 wt%, not more than 1.8 wt%, not more than 1.64 wt%, 1.6 wt% Or less, 1.4 wt% or less, 1.2 wt% or less, 1.0 wt% or less, 0.8 wt% or less, or 0.6 wt% or less.

In addition, the total amino acid content is determined by comparing the total amino acid content of the germinated soybean extract ("Third Party Soybean Extract" or "Third Party Soy Peptide Extract") treated with distilled water (or immersion) and boiled in place of the liquid obtained from immature fruit of the genus Citrus It can be more than three times the total amino acid content. Specifically, it may be 3 times or more, 4 times or more, 5 times or more, 6 times or more, 7 times or more, 7.5 times or more, 8 times or more, 8.2 times or more, 8.5 times or more, 9 times or more or 10 times or more.

In another aspect of the present invention, the content of BCAA may be 0.1 to 1.5% by weight based on the total weight of the extract. Leucine, isoleucine and valine are also referred to as branched-chain amino acids (BCAA). According to one embodiment, by treating a liquid obtained from immature fruit of a plant belonging to the genus Citrus, it is possible to increase the content of amino acids and increase the content of BCAA in the nutritional components of germinated beans. Specifically, the content of BCAA may be at least 0.1 wt%, at least 0.3 wt%, at least 0.5 wt%, at least 0.8 wt%, at least 1.0 wt%, at least 1.2 wt%, or at least 1.4 wt% based on the total weight of the extract, And may be 1.5 wt% or less, 1.3 wt% or less, 1.1 wt% or less, 1.0 wt% or less, 0.8 wt% or less, 0.6 wt% or less, 0.5 wt% or less, 0.3 wt% or less, or 0.2 wt% or less.

In addition, the content of BCAA may be 5 times or more as much as that of germinated soybean extract obtained by treating (or dipping) with distilled water instead of liquid obtained from immature fruit of Citrus plant. More specifically, at least 5 times, at least 7 times, at least 9 times, at least 10 times, at least 12 times, at least 15 times, at least 17 times, at least 19 times, at least 20 times, at least 22 times, at least 22.9 times, at least 24 times , 25 times or more, or 27 times or more.

In another aspect of the present invention, the content of leucine with respect to the total weight of the extract may be 0.03 to 0.1% by weight. Specifically, the content of leucine may be 0.03 wt% or more, 0.05 wt% or more, 0.06 wt% or more, 0.07 wt% or more, 0.072 wt% or more, 0.08 wt% or more, or 0.09 wt% 0.07 wt% or less, 0.08 wt% or less, 0.072 wt% or less, 0.07 wt% or less, 0.06 wt% or less, 0.05 wt% or less, or 0.04 wt% or less.

The leucine content may be 5 times higher than the leucine content of the germinated soybean extract obtained by treating (or dipping) with distilled water instead of the liquid obtained from the immature fruit of a plant belonging to the genus Citrus. Specifically, it may be 5 times or more, 7 times or more, 9 times or more, 10 times or more, 12 times or more, 13 times or more, 15 times or more, 18 times or more or 20 times or more.

In another aspect of the present invention, there is provided a germinated bean having a valine content of 0.01 to 0.5 wt% based on the total weight of the extract. Specifically, the content of valine is 0.01 wt% or more, 0.03 wt% or more, 0.05 wt% or more, 0.07 wt% or more, 0.09 wt% or more, 0.1 wt% or more, 0.121 wt% or more, 0.15 wt% or more, 0.2 wt% %, 0.4 wt% or less, 0.3 wt% or less, 0.2 wt% or less, 0.15 wt% or less, 0.121 wt% or less, 0.1 wt% or less, %, 0.09 wt% or less, 0.07 wt% or less, 0.05 wt% or less, 0.03 wt% or less, or 0.02 wt% or less.

In addition, the valine content of the germinated beans can be 5 times or more than the valine content of the germinated soybean extract that has been treated (or soaked) with distilled water and boiled instead of the liquid obtained from the immature fruit of a plant of the genus Citrus have. Specifically, it may be 5 times or more, 7 times or more, 9 times or more, 10 times or more, 12 times or more, 15 times or more, 17 times or more, 17.3 times or more, 18 times or more or 20 times or more.

In another aspect of the present invention, there is provided a composition wherein the content of isoleucine is 0.01 to 0.7% by weight, based on the total weight of the extract. Specifically, the leucine content may be 0.01 wt% or more, 0.03 wt% or more, 0.05 wt% or more, 0.08 wt% or more, 0.1 wt% or more, 0.2 wt% or more, 0.3 wt% or more, 0.31 wt% or more, 0.5% or less, 0.4% or less, 0.31% or less, 0.29% or less, 0.2% or less by weight, Or less, 0.1 wt% or less, 0.08 wt% or less, 0.05 wt% or less, 0.03 wt% or less, or 0.01 wt% or less.

In addition, the content of isoleucine may be 10 times or more as much as that of germinated soybean extract obtained by treating (or dipping) with distilled water instead of liquid obtained from immature fruit of a plant of the genus Citrus (Citrus) . More specifically, at least 10 times, at least 15 times, at least 18 times, at least 20 times, at least 23 times, at least 25 times, at least 28 times, at least 30 times, at least 32 times, at least 34 times, at least 34.4 times, at least 35 times , 40 times or more, or 45 times or more.

In another aspect of the invention, there is provided a composition wherein the content of GABA is 0.03 to 0.15% by weight, based on the total weight of the extract. Specifically, the content of the GABA may be 0.03 wt% or more, 0.04 wt% or more, 0.05 wt% or more, 0.054 wt% or more, 0.08 wt% or more, 0.1 wt% or more, 0.12 wt% or more, or 0.14 wt% 0.15 wt% or less, 0.12 wt% or less, 0.1 wt% or less, 0.08 wt% or less, 0.054 wt% or less, 0.05 wt% or less, or 0.04 wt% or less.

In addition, the content of GABA in the extract may be 2.5 times or more than that of the germinated soybean extract obtained by treating (or dipping) with distilled water instead of the liquid obtained from immature fruit of a plant of the genus Citrus. Specifically, it is 2.5 times or more, 2.8 times, 3.0 times, 3.5 times, 3.8 times, 4.0 times, 4.2 times, 4.5 times, 4.7 times, 5.0 times, 5.1 times, 5.5 times Or 6.0 times or more.

In another aspect of the present invention, there is provided a composition wherein the content of glutamic acid relative to the total weight of the extract is 0.05 to 0.4 wt%. More specifically, the content of glutamic acid may be 0.05 wt% or more, 0.07 wt% or more, 0.09 wt% or more, 0.1 wt% or more, 0.135 wt% or more, 0.2 wt% or more, or 0.3 wt% Or less, 0.3 wt% or less, 0.2 wt% or less, 0.15 wt% or less, 0.135 wt% or less, 0.1 wt% or less, 0.09 wt% or less, or 0.07 wt% or less.

In addition, the content of glutamic acid in the extract may be 5 times or more than the amount of glutamic acid in germinated soybean extract treated with distilled water (or dipped) and boiled in place of the liquid obtained from immature fruit of a plant of the genus Citrus. Specifically, it may be 5 times or more, 5.6 times or more, 5.8 times or more, 6 times or more, 6.5 times or more, 7 times or more, 7.2 times or more, 7.5 times or more, 7.7 times or more or 8 times or more.

In another aspect of the present invention, there is provided a composition wherein the content of phenylalanine is 0.01 to 0.5% by weight based on the total weight of the extract. Specifically, the content of the phenylalanine is 0.01% by weight, 0.05% by weight, 0.08% by weight, 0.1% by weight, 0.13% by weight, 0.15% by weight, 0.18% by weight, 0.2% by weight or more, 0.23% , Not more than 0.25 wt%, not more than 0.45 wt%, not more than 0.4 wt%, not more than 0.3 wt%, not more than 0.236 wt%, and more preferably not less than 0.236 wt%, not less than 0.3 wt%, not less than 0.4 wt% Or less, 0.23 wt% or less, 0.2 wt% or less, 0.18 wt% or less, 0.15 wt% or less, 0.13 wt% or less, 0.1 wt% or less, 0.08 wt% or less, or 0.05 wt% or less.

In addition, the content of phenylalanine in the extract may be 10 times or more as much as the content of phenylalanine in germinated soybean extract, which has been treated (or soaked) with distilled water and boiled, instead of the liquid obtained from immature fruit of a plant belonging to the genus Citrus. More specifically, it may be at least 10 times, at least 12 times, at least 15 times, at least 17 times, at least 20 times, at least 23 times, at least 26 times, at least 26.2 times, at least 28 times, at least 30 times, at least 32 times, .

In another aspect of the present invention, there is provided a composition wherein the content of alanine relative to the total weight of the extract is 0.01 to 0.5% by weight. Specifically, the content of alanine is 0.01 wt% or more, 0.03 wt% or more, 0.05 wt% or more, 0.07 wt% or more, 0.09 wt% or more, 0.1 wt% or more, 0.114 wt% or more, 0.15 wt% or more, %, 0.4 wt% or less, 0.3 wt% or less, 0.25 wt% or less, 0.2 wt% or less, 0.5 wt% or less, Or less, 0.114 wt% or less, 0.1 wt% or less, 0.09 wt% or less, 0.07 wt% or less, 0.05 wt% or less, or 0.03 wt% or less.

In addition, the alanine content of the extract may be three times or more as much as the alanine content of the germinated soybean extract that has been treated (or dipped) with distilled water instead of the liquid obtained from the immature fruit of a plant of the genus Citrus. Specifically, it may be 3 times or more, 4 times or more, 5 times or more, 6 times or more, 7 times or more, 8 times or more, 9 times or more, 10 times or more, 12 times or more or 15 times or more.

In accordance with one aspect of the present invention, there is provided a method for producing a fermented beverage, which comprises germinating soybeans immersed in a liquid obtained by juicing, heating and distilling immature fruit of a plant belonging to the genus Citrus, And then subjected to fermentation or hydrolysis with an enzyme derived from Bacillus genus (especially Bacillus subtilis) (protease), followed by concentration and sterilization, and then the effect on muscle cells was examined . The extract is derived from a liquid juice from germinated soybeans, that is, germination juice, and contains a large amount of amino acids and peptides as described above. The extracts containing such peptides (referred to herein as "germinated juice peptides") significantly enhance the amount of protein in muscle cells in a concentration-dependent manner and promote the phosphorylation of S6K in association with the mTOR-S6K pathway for protein synthesis (See Figs. 1 to 2). Thus, it has been clarified that the extract containing the germicidal peptide can be used for the enhancement of muscle strength or the improvement of muscle mass, thereby enabling the present specification to be used for preventing or treating muscle diseases caused by muscle wasting, muscle damage or muscle regeneration Respectively.

In addition, oral administration of the extract containing the germicidal peptide to a subject resulted in effects such as weight loss, improvement in abdominal obesity, decrease in body fat, decrease in blood glucose, and improvement in body shape, thereby preventing, treating and improving the metabolic syndrome. (See FIGS. 4 to 10), and that the present invention can also be utilized for such purposes by showing the effect of improving physical fitness and the like.

According to one embodiment, the content of the extract in the composition may be from 0.01% to 70% by weight, based on the total weight of the composition. The content may be at least 0.01 wt%, at least 0.05 wt%, at least 0.1 wt%, at least 0.5 wt%, at least 1 wt%, at least 5 wt%, at least 10 wt%, at least 15 wt% At least 30 wt%, at least 35 wt%, at least 40 wt%, at least 45 wt%, at least 50 wt%, at least 55 wt%, at least 60 wt%, or at least 65 wt%. Or the content is 70 wt% or less, 65 wt% or less, 60 wt% or less, 55 wt% or less, 50 wt% or less, 45 wt% or less, 40 wt% or less, 35 wt% or less, 30 wt% Up to 20 weight percent, up to 15 weight percent, up to 10 weight percent, up to 5 weight percent, up to 1 weight percent, up to 0.5 weight percent, up to 0.1 weight percent, or up to 0.05 weight percent.

According to another embodiment, the dose of the extract may be 100 mg / kg / day to 800 mg / kg / day. The dosage may be at least 100 mg / kg / day, at least 150 mg / kg / day, at least 200 mg / kg / day, at least 250 mg / kg / day, at least 300 mg / kg / day, at least 350 mg / kg / day, at least 450 mg / kg / day, at least 500 mg / kg / day, at least 600 mg / kg / day, at least 700 mg / kg / day, or at least 750 mg / kg / day. Alternatively, the dose may be in the range of 800 mg / kg / day, 750 mg / kg / day, 700 mg / kg / day, 650 mg / kg / day, 600 mg / kg / day, 550 mg / kg or less, 500 mg / kg / day, 450 mg / kg / day, 400 mg / kg / day, 350 mg / kg / day, 300 mg / kg / day, 250 mg / / Day, or 150 mg / kg / day or less.

According to another embodiment, the composition may be in a liquid phase. In the case of conventional protein supplements and muscle strengthening products, they are powder products, and in order to ingest them, they have to be mixed with liquid products such as purified water, milk, juice, and the like. The reason for this is that the stability of the ingredients is not maintained and the quality of the product is changed and the effect is lost. In addition, chemical reactions, aggregation, and component denaturation may occur during mixing with the liquid product and absorption inhibition may occur. According to one embodiment of the present invention, since the formulation can be stored and distributed in a liquid form, it is not necessary to separately perform a mixing process before ingestion, so that it is much easier to ingest than existing products, There is an advantage that there is no possibility of occurrence.

According to another embodiment, the composition may be a pharmaceutical composition or a food composition.

In one aspect of the invention, the pharmaceutical composition may further comprise, in one aspect, suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions. In one aspect, carriers, excipients and diluents that may be included in the composition include lactose, dextrose, dextrin, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium Silicates, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical composition may be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup, aerosol or the like, oral preparation, suppository and sterilized injection solution according to a conventional method .

In the case of formulation, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, a surfactant, or an excipient is usually used. Solid formulations for oral administration may include tablets, pills, powders, granules, capsules, and the like. Such solid preparations may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose, and gelatin in addition to the active ingredient. In addition to simple excipients, lubricants such as magnesium stearate talc may also be included. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, syrups and the like. In addition to water and liquid paraffin which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of suppositories include witepsol, macrogol, tween 61, laurin, cacao butter, glycerogelatin and the like.

The food composition may be in a liquid or solid form and may be in the form of, for example, various foods, beverages, gums, tea, vitamin complexes, health functional foods, health supplement foods, Can be used. The food composition of each formulation can be blended with the ingredients commonly used in the field in addition to the active ingredient without difficulty by those skilled in the art depending on the purpose of formulation or use, and synergistic effect can be obtained when the composition is applied simultaneously with other ingredients.

There are no particular limitations on the liquid components that can be contained in addition to the active ingredients disclosed in this specification, and may include various flavoring agents or natural carbohydrates such as ordinary beverages as additional ingredients. Examples of the natural carbohydrate include common saccharides such as disaccharides such as monosaccharide, glucose and fructose, polysaccharides such as maltose and sucrose, dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol . The above flavoring agents can be advantageously used as natural flavorings (tau martin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (for example, saccharin, aspartame etc.) The ratio of the natural carbohydrate may be generally about 1 to 20 g per 100 ml of the composition disclosed herein, and about 5 to 12 g per side.

In one aspect, the food composition may contain flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, colorants and heavies such as cheese and chocolate, pectic acid and its salts, alginic acid and its salts , Organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. On the other hand, flesh for the production of natural fruit juices and vegetable beverages. These components can be used independently or in combination. The proportion of the additive may vary, but is generally selected in the range of from 0.001 to about 20 parts by weight per 100 parts by weight of the composition disclosed herein.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for illustrating the present invention and that the scope of the present invention is not construed as being limited by these embodiments.

[Example 1] Preparation of foot citrus water

Pusan (immature state of Wonju citrus) was purchased from Ju Yeong International. The purchased mutton was washed with purified water to remove foreign matter and stored at low temperature (5-7 ° C) to prevent fruit maturation. The dried persimmon was juiced using a screw-type juicer and pressurized. Heat treatment was carried out at 70 캜 to inactivate the enzyme of the citrus fruit. The remaining solid was then removed by passing through a mesh of 18 to 30 mesh size. The juice solution from which the solid content was removed was placed in a vacuum concentrator and subjected to heat treatment at 30 mmHg at 100-121 ° C to obtain a distilled water.

[Example 2] Analysis of composition of foot water

GC analysis was carried out to identify the components of the foot water of Example 1.

Analysis method: GC / MS analysis, HS-SPME (Headspace Solid-Phase Microextraction)

Analysis conditions: Column: DB-WAX / Oven Condition: 50 (5min) ~ 240C / 3C / min (80min)

Inj .: 230 / Splitless / threshold: 16.5

The results of analyzing the components of the foot water are shown in Table 1.

NO R.T Volatile compounds Area ( % ) One 31.241 linalool 57.862% 2 31.696 alcohol C-8 4.648% 3 33.592 terpinene-4-ol 10.474% 4 37.175 terpineol 10.219% 5 39.656 citronellol 2.766% 6 40.803 nerol 1.259% 7 46.961 alcohol C-12 1.309% 8 50.621 elemol 5.143% 9 53.448 gamma-eudesmol 2.557% 10 53.998 alpha-cadinol 0.882% 11 55.061 alpha-eudesmol 1.136% KNOWN 98.255% UNKNOWN 1.745% TOTAL 100.00%

[Example 3] Production of germinated beans

- Immersion pretreatment: 200 g of myth bean was washed with 500 ml of distilled water (repeated twice). The washed soybeans were soaked in a 25 ° C incubator using 600 ml of immersion for 4 to 20 hours, in other embodiments immersed for 15 hours. In order to compare the case of using the seawater according to Example 1 and the distilled water according to Example 1, the sediment index was divided into the group immersed in 600 ml of the water of the citric acid according to Example 1 and the group using 600 ml of distilled water, Respectively. As another example, a mixture of green tea extract containing 2 to 5% by weight of the citrus fruit juice and 35% by weight of catechin was used as a needle index.

- Germination cultivator: The soybean immersion solution after the immersion was discarded, washed with 500 ml of distilled water (repeated twice), and the soybean was put into the germination tray of the soybean growing machine (Chungsilu, Shinchang, INC) and applied with a uniform thickness. 2000 ml of distilled water was added as germination water, and the soybean grower was placed in an incubator of 25 ° C for 10 to 100 hours for germination. The seeds were sprayed on the surface of soybeans for 1 minute every 20 minutes through a soybean grower.

- Replacement of germination during germination: If the germination time is more than 24 hours, the number of germination is changed once every 24 hours. That is, the germination solution that passed 24 hours was discarded, and the germination cultivation method was repeated while 2000 ml of germination solution was newly added.

- End of germination: All of the germination solution which germinated was discarded. After confirming the status of germinating beans, soybean was washed once with 2000 ml of distilled water, then soybean was immersed in 2000 ml of distilled water, and soybean enzyme was inactivated by bathing in a 60 ° C water bath for 1 hour.

[Example 4] Preparation of extract containing germinated juice peptide

200 kg of myth bean was germinated in accordance with the above Example 3. The germinated soybeans germinated for 1 day were processed in a conventional juicing process (the beans were not boiled, the beans were milled in a millstone, and the cloth was squeezed with a hand or an instrument After the juice was obtained through the process, the juice was obtained. Then, it is hydrolyzed (50 ° C., 16 hours) with an enzyme (protease derived from Bacillus genus, in particular Bacillus subtilis), concentrated and sterilized to obtain a germicidal peptide having a total content of water-soluble peptide and amino acid of 50% 250 kg of the extracted extract was obtained. The components of the extract were analyzed as shown in Table 2 below. The results of the analysis are shown in Table 3 below.

division Free amino acid Test Methods AccQ-Tag Amino Acid Assay Preparation of test liquid According to the AccQ-Tag method guidelines, device Waters Acquity (TM) UPLC system (Waters, Milford, Mass., USA) column Waters AccQ-Tag Ultra column (100 x 2.1 mm, 1.7 μm) Column temperature 55 ° C Dose 1 μL Flow rate 0.7 ml / min Mobile phase AccQ-Tag Ultra Eluent conc. A diluent, AccQ-Tag Ultra Elent B Mobile phase condition Concentration gradient solvent composition method

Free amino acid type % By weight to total weight% Compared with the case of using distilled water as a needle index, Histidine 0.038 4.8 Asparagine 0.06 6.0 Serine 0.049 7.0 Glutamine 0.013 6.5 Arginine 0.017 0.6 Glycine 0.036 18.0 Aspartic acid 0.08 40.0 Glutamic acid 0.135 5.6 Threonine 0.045 7.5 Alanine 0.114 6.0 γ-aminobutyric acid 0.054 2.8 Theanine 0.001 - Proline 0.023 5.8 Cystine 0.022 - Lysine 0.079 9.9 Tyrosine 0.026 2.6 Methionine 0.062 15.5 Valine 0.121 17.3 Leucine 0.072 12.0 Isoleucine 0.31 34.4 Phenylalanine 0.236 26.2 Tryptophan 0.053 5.3 Total free amino acid content (%) 1.646 8.2 BCAA content (%) 0.503 22.9

As can be seen from the above table, the total free amino acid content was 8.2 times higher and BCAA (branched chain amino acid) content about 23 times higher than that of distilled water. Thus, an extract containing a large amount of peptides derived from germinated juice (" germinated juice peptides ") was used in the following experimental examples.

[Experimental Example 1] Measurement of protein concentration

C2C12 muscle cells (American Type Culture Collection, Manassa, VA, USA) administration of 6-well plates in density of 1 x 10 ⁵ per well for each cell, and were cultured in DMEM medium containing 10% FBS (fetal bovine serum) , after 2% HS (horse serum) for 1 week. After completion of the differentiation, the medium was replaced with serum-free medium to eliminate the influence of other proteins and growth factors. Then, the extract containing the germinated juice peptide obtained according to Example 4, the soybean extract After 24 hours, proteins were extracted from muscle cells using radioimmunoprecipitation assay buffer (RIPA buffer) and the amount of protein in muscle cells was measured by BCA assay. That is, 1 ml of each sample was mixed with 200 ml of BCA soultion 1: BCA solution 2 = 50: 1, incubated for 30 minutes, and absorbance was measured at 562 nm to quantitate the protein.

As a result, as shown in FIG. 1, it was confirmed that the protein concentration increases as the concentration of the germinated peptide-containing extract increases (the number of the X-axis in FIG. 1 indicates ppm). In addition, it was found that the extract containing the germinated juice peptide obtained according to Example 4 significantly increased the protein concentration in the muscle cells, as compared with the extract containing the non-soybean soybean peptides without the use of foot water.

[Experimental Example 2] Phosphorylation confirmation of S60K

After the serum-free media treatment of the differentiated C2C12 myocytes as described in the above Experimental Example, leucine was added to each muscle cell, the extract containing the germicide peptide obtained according to Example 4, and a soybean extract The leucine concentration was treated to be equal to the leucine concentration contained in the 1,000 / 5,000 ppm extract of the germinated juice peptide (e. G., The germicidal peptide < RTI ID = 0.0 > Containing 4% leucine at 1,000 / 5,000 ppm, leucine is treated at 40, 200 ppm respectively).

Thereafter, the phosphorylation of S60K directly linked to the mTOR signal was confirmed. That is, the cells were dissolved with 200 ml of RIPA buffer, centrifuged at 4,000 rpm for 15 minutes at 12,000 rpm, and the supernatant was collected. After quantification of the protein using the BCA assay as described above, 40 mg of western blot was performed. The results are shown in Fig. In other words, S6K phosphorylation was observed in a concentration-dependent manner when the extract containing germinated juice peptides ("germinated soybeans" in the figure) was treated. As a result, it was found that the mTOR pathway was activated, (In the figure, " other soybean "), the degree of phosphorylation of S6K was insignificant compared to the extract containing germinated juice peptide and leucine, indicating that mTOR pathway activation was weak. The extracts containing germinated squalene peptides increased S6K phosphorylation compared to the same contents of leucine. From this point, it can be seen that the germinal juice peptide-containing extract will enhance muscle cell growth. In FIG. 2, 1 indicates the group treated with nothing, 2 indicates the group treated with leucine at 40 ppm, 3 indicates the group treated with leucine at 200 ppm, and 4 through 6 indicates the groups using 100 ppm, 1000 ppm, , 7 to 9 are the groups in which the extracts containing the germicidal peptide were used at 100 ppm, 1000 ppm and 5000 ppm, respectively, and p-S6K represents phosphorylated S6K.

[Experimental Example 3] Tests and questionnaires for subjects

The effect of the germicidal peptide-containing extract of Example 4 was confirmed on the subjects.

Specifically, the subjects were recruited from the age of 45 to 65 years, and the SMI (skeletal muscle mass index) was less than 6.75 (kg / m ² ) and the BMI (body mass index) 24.0 ~ 29.9 are ^{kg / m 2, WHR (waist} hip Ratio, waist / hip size) is 0.85 or higher were selected as subjects for 10. SMI is an appendicular lean mass / height ² The values of SMI, WHR, and BMI were measured using a body composition analyzer (Invadia, Korea). The values were measured using the numerical value (appendicular lean mass represents fat, height ² represents the square of the key).

10 selected subjects were orally administered the composition containing the germicidal peptide-containing extract (0.5% or 200 mg of the extract in the composition) (8-week, twice daily, 40 ml or 40 g of the composition per day) As a result, it was confirmed that the waist circumference, BMI, body fat mass, percent body fat, and WHR were significantly decreased as shown in Fig. 4 ("Baseline" indicates the values measured before the administration of the composition containing the germicidal peptide- will be).

Fig. 5 shows the results of measurement of muscle mass of the left and right legs. As a result of oral administration of the germicidal peptide-containing extract for 8 weeks, it was confirmed that the lower body muscle mass and SMI were significantly increased (SMI (%) means skeletal muscle mass / body mass X 100, LM means lean mass or fat mass)

Fig. 6 shows the result of confirming the sieve structure change. As a result of administration of the germicidal peptide - containing extract, the body weight and fat mass were decreased, the muscle mass and fat mass were increased, and the skeletal muscle amount relative to body weight was also increased.

As a result of comparing the exercise capacity and flexibility before and after administration of the germicidal peptide-containing extract, the results of the questionnaire showed that the muscle function, cardiopulmonary function and flexibility were remarkably changed, (Fig. 7 and Fig. 8, where the relative grip force is the gripping force (kg) / body weight (kg) X 100). In FIG. 7, the test for the pull-up refers to a sit-up.

Meanwhile, as shown in FIG. 9, the extracts containing the germinated juice peptides were found to be effective in the improvement of the metabolic syndrome-related index, and furthermore, it was found to be effective in the prevention, improvement and treatment of the metabolic syndrome.

The result of checking the effect of the subject on the questionnaire is shown in Fig. That is, the subjects perceived weight loss, body fat reduction, and muscle strength improvement by administration of the germicidal peptide-containing extract, and the fatigue improvement and the condition improvement were satisfactory.

Formulation examples of compositions according to one aspect of the present invention are described below, but may be applied to various other formulations, which are not intended to be limiting but merely illustrative of the invention.

 [Formulation Example 1] RTD (ready to drink)

600 mg, 80 mg, and 40 mg (respectively, 1.75%, 1.5%, 0.2%) of the mixture of the extract of the soybean embryo extracted with hot water and the mixture of L-carnitine in a weight ratio of 4: 3 (APIC), leucine, phenylalanine and methionine %, 0.1% by weight). The extract was prepared in the form of a liquid by mixing the appropriate amount of the extract of Example 4 and other additional raw materials, and was made into a ready-to-drink (RTD) form.

[Formulation Example 2] Soft capsule

A soft capsule was prepared by mixing 200 mg of the extract of Example 4, 80 to 140 mg of L-carnitine, 180 mg of soybean oil, 2 mg of palm oil, 8 mg of vegetable hydrogenated oil, 4 mg of yellow radish and 6 mg of lecithin and filling them into 1 capsule according to a conventional method.

[Formulation Example 3] Tablets

200 mg of the extract of Example 4, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluidized bed drier, followed by addition of 6 mg of sugar ester and tabletting by tableting.

[Formulation Example 4]

200 mg of the extract of Example 4, 250 mg of anhydrous crystalline glucose, and 550 mg of starch were mixed and granulated into granules using a fluidized bed granulator, followed by filling the granules.

[Formulation Example 5] Health food

Healthy foods were prepared in a conventional manner according to the compositions shown in Table 4 below.

ingredient content The extract of Example 4  200 mg Vitamin mixture Vitamin A acetate 70 [mu] g Vitamin E 1.0 mg Vitamin B1 0.13 mg Vitamin B2 0.15 mg Vitamin B6 0.5 mg Vitamin B12 0.2 [mu] g Vitamin C 10 mg Biotin 10 [mu] g Nicotinic acid amide 1.7 mg Folic acid 50 [mu] g Calcium pantothenate 0.5 mg Mineral mixture Ferrous sulfate 1.75 mg Zinc oxide 0.82 mg Magnesium carbonate 25.3 mg Potassium monophosphate 15 mg Dicalcium phosphate 55 mg Potassium citrate 90 mg Calcium carbonate 100 mg Magnesium chloride 24.8 mg

Although the composition ratio of the vitamin and the mineral mixture is relatively mixed with the ingredient suitable for health food, the compounding ratio of the vitamin and the mineral mixture may be arbitrarily varied. The ingredients may be mixed according to a conventional method for producing health food, Can be used for the production of health food compositions.

[Formulation Example 6] Health drinks

ingredient content The extract of Example 4  200 mg Citric acid 1000 mg oligosaccharide 100 g Plum concentrate 2 g Taurine 1 g Purified water Balance Total volume 900 ml

As shown in Table 5, a remaining amount of purified water was added to a total volume of 900 ml, and the above components were mixed according to a conventional health drink manufacturing method. After stirring for about 1 hour at 85 캜, the solution was filtered Sterilized 2 liter container, sealed sterilized, and stored in a refrigerator to prepare a health drink.

Having described specific portions of the present invention in detail, it will be apparent to those skilled in the art that this specific description is only a preferred embodiment and that the scope of the present invention is not limited thereby. It will be obvious. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (25)

A composition for enhancing muscle strength or muscle mass comprising, as an active ingredient, an extract obtained from an immature fruit of a plant belonging to the genus Citrus, or an extract derived from a soybean treated with the composition. 2. The composition of claim 1, wherein the muscle strength enhancement or muscle mass enhancement is by increasing the amount of protein in the muscle cells. 2. The composition of claim 1, wherein said muscle strength enhancement or muscle mass enhancement is by activation of mTOR pathway in muscle cells. 2. The composition of claim 1, wherein the muscle strength enhancement or muscle mass enhancement is by S6K phosphorylation enhancement, a protein synthesis signal in muscle cells. A composition for the prevention or treatment of muscular diseases caused by muscle wasting, muscle damage or muscle degeneration comprising an extract obtained from an immature fruit of a plant of the genus Citrus or an extract extracted from a soybean treated with the composition. A composition for the prevention or treatment of metabolic syndrome comprising as an active ingredient an extract obtained from immature fruit of a plant of the genus Citrus or a soybean extract obtained from the processed liquid. 7. The composition of claim 6, wherein the prevention or treatment of metabolic syndrome is by at least one selected from the group consisting of weight loss, abdominal obesity improvement, body fat reduction, hypoglycemia, and body improvement. A composition for enhancing physical fitness comprising as an active ingredient an extract from a liquid obtained from immature fruit of a plant of the genus Citrus or from a soybean treated with the processed product. The composition according to claim 8, wherein the physical fitness is at least one selected from the group consisting of cardiopulmonary function enhancement, flexibility enhancement, and fatigue relief. 10. The composition according to any one of claims 1 to 9, wherein the liquid is a liquid obtained by distilling and liquefying a liquid juice in immature fruit. 10. The composition according to any one of claims 1 to 9, wherein the liquid is an enzyme in which the immature fruit enzyme is inactivated. 10. The process according to any one of claims 1 to 9, wherein the liquid or the workpiece is selected from the group consisting of linalool, terpinene-4-ol, terpineol, ≪ / RTI > citronellol, and the like. 10. The composition according to any one of claims 1 to 9, wherein the treatment is carried out with a liquid obtained from immature fruit of a plant of the genus Citrus or a workpiece thereof. 10. The method according to any one of claims 1 to 9, wherein the Citrus genus is selected from the group consisting of pomelo, citron, mandarin, tangerine, banana, lime, grapefruit, lemon, sour orange, citron, mandarin orange, / RTI > 10. The composition according to any one of claims 1 to 9, wherein the Citrus plant is citrus reticulate or satsuma mandarin. 10. The composition according to any one of claims 1 to 9, wherein the soybean is a soybean germinated after the treatment. 10. The composition according to any one of claims 1 to 9, wherein the soybean is a mythic bean. 10. The composition according to any one of claims 1 to 9, wherein the extraction comprises extraction after milling or squeezing. 10. The method according to any one of claims 1 to 9, wherein the composition further comprises at least one selected from the group consisting of a mixture of L-carnitine, leucine, phenylalanine, and methionine, , Composition. 10. The composition according to any one of claims 1 to 9, wherein the extract has one or more of the following characteristics.
a) the content of total free amino acids is from 0.4 to 2.5% by weight, based on the total weight of the extract;
b) 0.1 to 1.5% by weight, based on the total weight of the extract, of BCAA;
c) the content of leucine is 0.03 to 0.1% by weight based on the total weight of the extract;
d) 0.01 to 0.5% by weight, based on the total weight of the extract, of valine;
e) the content of isoleucine is from 0.01 to 0.7% by weight, based on the total weight of the extract;
f) 0.03 to 0.15% by weight, based on the total weight of the extract, of GABA;
g) 0.05 to 0.4% by weight, based on the total weight of the extract, of glutamic acid;
h) 0.01 to 0.5% by weight, based on the total weight of the extract, of phenylalanine; And
i) 0.01 to 0.5% by weight, based on the total weight of the extract, of alanine. 10. The composition according to any one of claims 1 to 9, wherein the content of the extract in the composition is from 0.01% to 70% by weight, based on the total weight of the composition. 10. The composition according to any one of claims 1 to 9, wherein the dose of the extract is from 100 g / kg / day to 800 mg / kg / day. 10. The composition according to any one of claims 1 to 9, wherein the composition is in a liquid phase. 10. The composition according to any one of claims 1 to 9, wherein the composition is a pharmaceutical composition. 10. The composition according to any one of claims 1 to 9, wherein the composition is a food composition.

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