KR102552886B1 - Composition for preventing and improving obesity, and functional food containing the same - Google Patents

Abstract

The present invention relates to a composition for preventing and improving obesity and a functional food containing the same. The present invention provides a composition based on a natural extract capable of exhibiting an excellent activity in preventing or improving obesity, which reduces body weight and body fat, and improves blood lipid levels by regulating the expression of transcription factors related to adipocyte differentiation.

Description

Composition for preventing and improving obesity and functional food containing the same

The present invention relates to a composition for preventing and improving obesity and a functional food containing the same. More specifically, the present invention relates to a composition for preventing and improving obesity, which regulates the expression of transcription factors related to differentiation of adipocytes, thereby suppressing differentiation of adipocytes and inducing reduction of body weight and body fat, and a functional food containing the same It is about. In particular, it has the advantage that there is no problem such as side effects due to long-term use by using ingredients derived from natural products.

Obesity refers to a state in which body fat is excessive, and can be defined as a health risk state that causes various diseases including heart disease due to excessive body fat. The World Health Organization (WHO) reports that there are approximately 106 million overweight adults worldwide, at least 4 million are obese, and more than two out of three Americans are overweight or obese (Low et al. al, 2009; Cooke and Bloom, 2006). It has also been reported that more than 20% of adults in the UK are diagnosed with obesity. Even in Korea, the proportion of the obese population was 13.9% in 1995 and 30.6% in 2001.

It is increasing rapidly (Korean Society for Obesity, 2010). Compared to people of normal weight, obese people are at increased risk of various diseases, including type 2 diabetes, hyperlipidemia, arthritis and apnea, and are known to cause several types of cancer and heart disease in particular. The US National Institutes of Health (NIH) reported that obesity is responsible for 20% of all deaths, which is already more than the number of deaths caused by tobacco (Shi and Burn, 2006).

Although it is difficult to define that overweight and obesity originate from a single cause, they are basically caused by consuming more calories than the body consumes (NIH, 2010). The causes can be largely divided into endocrine factors, genetic factors, and socio-environmental factors, but obesity due to secondary factors such as endocrine diseases or genetic factors is less than 5%, and most of them are simple obesity, not pathological causes. It can be said to be caused by eating habits that exceed the amount of calories consumed (Korean Society for Obesity, 2010).

The causes of obesity or overweight (hereinafter referred to as 'obesity' throughout this specification) are most often due to lifestyles such as excessive nutritional intake and lack of physical activity, and rarely due to drug use or disease. may occur intermittently.

Obesity can cause serious adult diseases such as diabetes, cardiovascular disease, hypertension, and hyperlipidemia as well as its own problems, and since obesity increases the prevalence of various cancers, much attention is being paid to treatment.

According to statistics from the World Health Organization (WHO) in 2014, 1.9 billion adults over the age of 18 worldwide are overweight, of which 600 million have reached obesity. Obesity is one of the leading factors increasing the incidence of cardiovascular disease, type 2 diabetes and several types of cancer, with over 2.8 million deaths each year due to being overweight or obese.

In addition, as the standard of living of modern people has improved, the fat intake of Koreans has increased by 5.9g over the past 10 years, and the prevalence of obesity has increased by 1.9% compared to 2005. In particular, the prevalence of male obesity has increased significantly to 39.7%, and the prevalence of hypercholesterolemia has increased by 9.9% to 17.9%. Obesity is recognized as a disease to be treated and treated as a global nutritional problem.

As a method for improving obesity, in addition to surgical treatment, exercise therapy, diet therapy, behavior therapy, and drug therapy using an appetite suppressant, a laxative, or fiber to give a feeling of fullness are currently known. Recently, research on the development of drug treatment for the treatment of obesity has been conducted, but drug treatment drugs developed so far are known to have side effects such as headache, insomnia, nervous anxiety, hypertension, abdominal pain, abdominal distension, and increased defecation compared to efficacy.

Drug treatment is largely divided into appetite suppressants and fat absorption inhibitors depending on the mechanism of action. However, sibutramine, which suppresses appetite, has cardiovascular side effects, and drugs such as phentermine and diethylpropion have side effects such as increased blood pressure, dizziness, headache, tremor, and dry mouth. In the case of orlistat, a fat absorption inhibitor, the absorption of fat-soluble vitamins is inhibited, and steatorrhea, fat excretion, frequent bowel movements, and fecal incontinence may occur.

Due to the side effects of these drug treatments, the development of new anti-obesity functional foods and medicines is required, and recently, research and development have been conducted to find functional materials effective for preventing and improving obesity from natural materials and to reveal the mechanism of action.

Currently, attempts are being made to find a composition for preventing or treating obesity and overweight all over the world, and representatively, research on isolating a natural composition from a plant is in progress. Research to find a composition for preventing or treating obesity and overweight from natural products is expanding from medicinal plants to wild vegetables, vegetables and fruits in Northeast Asia including Korea.

(Patent Document 1) KR 10-1595515 B1 (Patent Document 2) KR 10-2015-0055876 A1

An object of the present invention is to provide a composition for the prevention and improvement of obesity containing an extract of Arabidopsis, and a functional food containing the same.

An object of the present invention is to provide a natural extract-based composition capable of exhibiting an excellent activity in preventing or ameliorating obesity, reducing body weight and body fat, and improving blood lipid levels by regulating the expression of transcription factors related to adipocyte differentiation.

Another object of the present invention is to provide a food composition of various formulations based on a natural extract-based composition that can be taken or used for a long time without problems such as side effects and has effects for preventing and improving obesity.

In order to achieve the above object, the composition for preventing and improving obesity according to an embodiment of the present invention is to include an extract of A.

The composition of the extract may inhibit the expression of PPARγ and C/EBPa.

The extract may be extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohol, and mixtures thereof.

The extract is Lactobacillus casei; Lactobacillus rhamnosus; Bifidobacterium bifidum Bifidobacteria; Bifidobacterium breve Bifidobacteria; Lactobacillus acidophilus; Any one selected from the group consisting of Bacillus subtilis and mixtures thereof may be inoculated and fermented.

The composition for preventing and improving obesity may further include a lotus flower extract or an artichoke extract.

Food for preventing and improving obesity according to another embodiment of the present invention may contain the composition.

A functional food for preventing and improving obesity according to another embodiment of the present invention may contain the above composition.

Hereinafter, the present invention will be described in more detail.

A composition for preventing and improving obesity according to an embodiment of the present invention includes an extract of A.

The baby swimming is native to Europe. It grows on roadsides or clearings. The rhizome grows sideways, the stem stands straight, the height is 20-50cm, there are hair-like protrusions, there are angular vertical lines, and it is purple and has a sour taste along with the leaves. The leaves from the root are grouped together, the petiole is long, it is spear-shaped, and the tip is sharp, and projections like ears spread to the left and right at the bottom. The leaves attached to the stem are alternate and have the same shape as the leaves from the root, but are smaller in size. The flowers are hermaphroditic, bloom in reddish green in May-June, and run in panicle at the end of the stem. The male flower is 3mm in diameter and has 6 sepals and 6 stamens each. The female flower is smaller than the male flower, has 6 calyx pieces, 1 pistil, and has a style split into 3 pieces and a finely split stigma. The fruit is an achene, 2mm long, elliptical, brown, and has 3 angular lines.

As used herein, the term 'extract' has the meaning commonly used as a crude extract in the sugar leaf system as described above, but also includes fractions obtained by further fractionation of the extract in a broad sense. That is, the extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through the purification method are also included in the extract of the present invention.

The extract used in the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

The Arabidopsis extract according to the present invention can exhibit an effect of preventing and improving obesity by inhibiting mast cell differentiation through anti-obesity activity.

The composition of the extract may inhibit the expression of PPARγ and C/EBPa.

The PPARγ (Peroxisome proliferator activated receptor γ) and C/EBPα (CCAAT-enhancer-binding protein α) are important factors involved in the fat cell formation process, and PPARγ forms a heterodimer with retinoid X receptor (RXR) It is a transcription factor that regulates the expression of target genes.

These two factors increase gene expression by C/EBPs, which are expressed early in differentiation, and regulate the expression of adipogenic genes such as adiponectin and glucose transporter 4 (GLUT4) during adipocyte differentiation. .

Accordingly, the composition can exhibit excellent effects of reducing body weight and body fat and improving blood lipid levels by suppressing the expression of PPARγ and C/EBPa.

The extract may be extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohol, and mixtures thereof.

A method for preparing the extract may be a conventional extraction method in the art, such as an ultrasonic extraction method, a leaching method, and a reflux extraction method. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances are removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract obtained by sequentially applying the solvents to a sample.

The reflux extraction method is based on 100 mL of water and alcohol having 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, 1 to 3 hours of reflux time, and 50 to 100% of alcohol having 1 to 6 carbon atoms or water. More specifically, based on 100 Ml of alcohol having 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of pulverized natural product, 1 to 2 hours of reflux, and 70 to 90% of alcohol or water having 1 to 4 carbon atoms.

The leaching method is carried out at 15 to 30 ° C. for 24 to 72 hours, and water or 50 to 100% of alcohol having 1 to 6 carbon atoms is used as an extraction solvent. More specifically, it is performed at 20 to 25 ° C. for 30 to 54 hours, and the extraction solvent is water or 70 to 80% of alcohol having 1 to 6 carbon atoms.

In the ultrasonic extraction method, the reaction is performed at 30 to 50 ° C. for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% of alcohol having 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50 ° C. for 1 to 2.5 hours, and water or 70 to 80% of alcohol having 1 to 6 carbon atoms is used as an extraction solvent.

The extraction solvent may be used in an amount of 2 to 50 times, more specifically, 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for leaching in the extraction solvent for 1 to 72 hours, more specifically 24 to 48 hours.

After extraction, the extract can be fractionated by sequentially applying a fresh fractionation solvent. The fractionation solvent used in the fractionation is at least one selected from the group consisting of water, hexane, butanol, ethylacetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, a method such as concentration or lyophilization may be additionally used.

The extract is Lactobacillus casei; Lactobacillus rhamnosus; Bifidobacterium bifidum Bifidobacteria; Bifidobacterium breve Bifidobacteria; Lactobacillus acidophilus; Any one selected from the group consisting of Bacillus subtilis and mixtures thereof may be inoculated and fermented.

The composition for preventing and improving obesity may further include a lotus flower extract or an artichoke extract.

Preferably, the composition for preventing and improving obesity includes an extract of Codfish, and a lotus extract for the Codfish extract; Artichoke extract; noni extract; Chazigi extract may be included. In the case of the combination, the activity of the active ingredient exhibiting the anti-obesity effect is enhanced according to the synergistic effect caused by the interaction of each extract to exhibit a better obesity prevention or improvement effect. can In particular, a unique synergistic effect that does not appear in the case of mixing of other plant extracts can be confirmed.

More preferably, the composition for preventing and improving obesity includes 1 to 5 parts by weight of lotus flower extract and 10 to 30 parts by weight of arboreal extract, based on 100 parts by weight of the baby swimming extract; It may further include 1 to 5 parts by weight of noni extract and 5 to 15 parts by weight of chazuki extract. In the case of the above range, anti-obesity activity may be very excellent by suppressing the activity of mast cell differentiation mediators. In particular, through the excellent anti-obesity effect that cannot be found in the mixture of other extracts or other mixture ranges, better obesity prevention and improvement effects can be exhibited.

Food for preventing and improving obesity according to another embodiment of the present invention may contain the composition.

A functional food for preventing and improving obesity according to another embodiment of the present invention may contain the above composition.

The functional food for preventing and improving obesity is Schizandra extract, strawberry extract, acai berry extract, aronia extract, blueberry extract, cranberry extract, grapefruit extract, orange extract, lemon extract, chocolate mint extract, basil extract, peppermint extract, sage It may include any one selected from the group consisting of extract, lavender extract, parsley extract, gompi extract, windbreak extract, coltsfoot extract, wild flower extract, and mixtures thereof.

As a specific example, a processed food capable of enhancing the effect of preventing and improving obesity can be prepared using the food composition. Such processed foods include, for example, sweets, tea, beverages, alcoholic beverages, fermented foods, canned foods, processed milk products, processed meat products, noodles, and the like. Sweets include biscuits, pies, cakes, breads, candies, jellies, chewing gum, cereals, and the like. Beverages include drinking water, carbonated beverages, functional ionic beverages, functional ionic beverages, juice (eg, apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), sikhye, and the like. Liquor includes rice wine, whiskey, soju, beer, liquor, fruit wine, and the like. Fermented foods include soy sauce, soybean paste, red pepper paste, and the like. Canned food includes canned seafood (e.g., canned tuna, mackerel, saury, canned conch, etc.), canned livestock products (canned beef, pork, chicken, turkey, etc.), canned agricultural products (canned corn, peaches, canned pineapple, etc.). Milk processed foods include cheese, butter, yogurt, and the like. Processed meat products include pork cutlet, beef cutlet, chicken cutlet, and sausage. Includes sweet and sour pork, nuggets, nubani, etc. It includes noodles such as sealed packaged raw noodles. In addition to this, the composition can be used for retort food, soup, and the like.

The food for treating and improving obesity may be provided in a tea formulation and include the composition for treating and improving obesity.

When using the health functional food composition of the present invention as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. Active ingredients can be appropriately used depending on their purpose of use (prevention or improvement). In general, when preparing food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of health functional food. The health functional food composition of the present invention can be made into food, particularly functional food. The functional food of the present invention includes components commonly added during food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, natural carbohydrates or flavors may be included as additional ingredients in addition to active ingredients. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg, maltose, sucrose, etc.) , xylitol, sorbitol, erythritol, etc.) are preferred. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonic acid It may further contain a carbonation agent used in beverages and the like.

"Functional food" as defined herein means food manufactured and processed using raw materials or ingredients having useful functionality for the human body in accordance with Functional Food Act No. 6727, and "functional" refers to the structure and It refers to intake for the purpose of obtaining useful effects for health purposes such as regulating nutrients for functions or physiological functions.

The present invention provides a composition for the prevention and improvement of obesity containing an extract of Arabidopsis and functional food containing the same.

The present invention provides a composition based on a natural extract capable of exhibiting an excellent activity in preventing or improving obesity, which reduces body weight and body fat, and improves blood lipid levels by regulating the expression of transcription factors related to adipocyte differentiation.

The present invention is a natural extract-based composition that can be taken or used for a long time without problems such as side effects, and provides a food composition of various formulations based on the effect of preventing and improving obesity.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Preparation Example 1: Preparation of Aegyphia extract]

1. Preparation of Arabidopsis extract and fermented extract of Arabidopsis

The young shoots of Arabidopsis were washed, dried, pulverized, extracted with hot water, and concentrated and filtered to prepare an extract (HW) of Arabidopsis. In addition, in order to confirm the synergistic effect through the fermentation process for the extract of Suyeong, fermentation was performed for the following [Table 1]. The synergistic effect was confirmed while inoculating S1 (Lactobacillus casei), S2 (Bifidobacterium bifidum bifidus), and S1 and S2 together as fermentation (S) strains.

H1 H2 H3 extract HW HW HW S S1 S2 S1+S2

The following experiments were conducted while diluting the Arabidopsis extract or fermented Arabidopsis extract at a constant concentration.

2. Preparation of Mixed Extracts

Lotus extract (AD1) through hot water extraction according to the same method as above and HW; Artichoke extract (AD2); Noni extract (AD3) and chazuki extract (AD4) were prepared, and a mixed composition was prepared according to the composition of each extract as shown in [Table 2] below.

M1 M2 M3 M4 M5 M6 M7 M8 HW 100 100 100 100 - - - - H3 - - - - 100 100 100 100 AD1 0.1 One 5 10 0.1 One 5 10 AD2 One 10 30 40 One 10 30 40 AD3 0.1 One 5 10 0.1 One 5 10 AD4 One 5 15 20 One 5 15 20

(unit: parts by weight)

[Experimental Example 2: Assessment of obesity treatment and improvement effects]

1. Assessment of 3T3-L1 cell viability

To investigate the cytotoxicity of each extract in 3T3-L1 adipocytes, MTT assay was performed at a concentration of 500 μg/ml. Purified water was used as a control, and the effect of each composition on cell viability was evaluated by comparing and evaluating the degree of cell proliferation of the control group. 3T3-L1 preadipocytes were dispensed into a 6-well plate for cell culture, treated with each example, cultured, and then, after removing the MTT reagent, the absorbance was measured at 540 nm with an ELISA reader.

The results are shown in [Table 3] below.

Cell viability (%) Con HW H1 H2 H3 M1 M2 M3 M4 M5 M6 M7 M8 100 98 96 95 92 92 90 91 90 91 92 90 91

Referring to [Table 3], it can be seen that each preparation example shows a cell viability of 90% or more compared to the control group, and there is no problem of cytotoxicity according to the use of each example.

2. Evaluation of 3T3-L1 cell differentiation inhibitory effect

In order to confirm the anti-obesity activity according to each preparation example, that is, the effect of inhibiting 3T3-L1 cell differentiation, the effect on adipocyte differentiation and triglyceride accumulation of pre-adipocytes according to each example was tested. After treatment with the composition according to each preparation example, differentiation from preadipocytes to mature adipocytes was induced. After differentiation was induced, the degree of fat formation was observed under a phase contrast microscope before and after Oil Red O staining. For objective comparison, the degree of differentiation was compared with the control group treated with purified water and evaluated on a scale of 1 to 10, and the results are shown in [Table 4] below. The lower the index, the better the anti-obesity activity.

Inhibit cell differentiation activation Con HW H1 H2 H3 M1 M2 M3 M4 M5 M6 M7 M8 10 7 8 7 5 7 5 5 7 6 3 3 5

(Unit: index)

Referring to [Table 4], it can be confirmed that differentiation of preadipocytes into adipocytes is inhibited when pretreatment is performed with the composition according to the preparation example of the present invention. In particular, in the case of the mixed composition, it can be confirmed that in the case of the combination range of M2 and M3, an additional synergistic effect is achieved unlike other composition ranges due to the interaction of each active ingredient.

In addition, in the case of the fermented composition, it was confirmed that the adipocyte differentiation inhibitory activity was excellent in the case of H3. In addition, referring to M6 and M7, it can be confirmed that additional synergistic activity appears in the range of combinations of M6 and M7 even in the case of the fermentation composition.

Therefore, it can be seen that the anti-obesity effect can be achieved by effectively suppressing the differentiation of pre-adipocytes into mature adipocytes in the case of the above composition. Accordingly, it is possible to achieve an effect of preventing obesity or improving obesity based on the composition.

3. Evaluation of PPARγ and C/EBPα expression inhibition activity

3T3-L1 was treated with Preparation Example and cultured. After obtaining the lysate by treatment with cell lysis buffer, protein was quantified, and the PVDF membrane on which the separated protein was transferred was treated with 5% skim milk to perform blocking for non-specific proteins, and Western blot analysis was performed. The results were visually observed, and the purified water treatment group was evaluated on a scale of 1 to 10 compared to the control group, and is shown in [Table 5] below. The higher the index, the higher the activity of the adipocyte differentiation mediator, and the better the anti-obesity effect.

Con HW H1 H2 H3 M1 M2 M3 M4 M5 M6 M7 M8 PPARγ One 3 3 3 5 2 6 5 3 5 7 7 5 C/EBPα One 4 3 3 5 3 6 7 3 5 7 8 4 C/EBPβ One 4 4 4 5 4 7 7 4 4 8 8 5

(Unit: Index)

Many types of adipogenic transcription factors act in the process of adipogenesis, which differentiates preadipocytes into adipocytes. Among them, C/EBPβ is a representative transcription factor regulating C/EBPα and PPARγ, and PPARγ and C/EBPα are key transcription factors regulating adipogenesis. It is known to induce the expression of These differentiated cells have the characteristics of adipocytes by inducing the expression of specific genes along with morphological characteristics such as lipid droplet generation and cell size increase. Therefore, according to the above experiments, it was confirmed at the protein level how the composition according to each preparation had an effect on the expression of adipogenic transcription factors.

Referring to [Table 5], it can be seen that the expression of PPARγ, C/EBPα and C/EBPβ decreased at the protein level when the composition according to the preparation example was treated in the differentiation induction process compared to the control group. Through the above results, the composition according to the preparation example effectively suppresses the expression of adipogenic transcription factors PPARγ, C/EBPα and C/EBPβ in 3T3-L1 pre-adipocytes, thereby reducing lipid droplet and TG production, thereby reducing fat cell It can be confirmed that there is an effect of inhibiting differentiation into .

In the case of the mixed composition, it can be confirmed that the synergistic effect appears in the case of the composition of M2 and M3, and in the case of the fermentation composition, the effect is more excellent in the case of H3, and in the case of the mixture of the fermentation composition, M6 and M7 composition An additional synergistic effect can be confirmed in the range.

Therefore, in the case of the above range, it may be possible to take or use for a long time with better activity even with a smaller content, and it may be provided in various formulations and replenished.

[Preparation Example 2: Preparation of tea beverage formulation]

The HW, H3, M3, and M6 are used to prepare basic tea beverages, and additionally, Schisandra chinensis extract (E1), cranberry extract (E2), peppermint extract (E3), parsley extract (E4), and wild flower extract (E5) are prepared, The HW, H3, M3, and M6 were mixed as shown in [Table 6] to prepare a food as a tea beverage formulation.

T1 T2 T3 T4 T5 T6 T7 HW 100 100 100 100 - - - H3 - - - - 100 - - M3 - - - - - 100 - M6 - - - - - - 100 E1 0.01 0.1 One 3 One One One E2 0.1 One 3 5 3 3 3 E3 0.01 0.1 2 5 2 2 2 E4 One One 3 5 3 3 3 E5 0.1 0.1 2 5 2 2 2

(unit: parts by weight)

[Experimental Example 2: Evaluation of palatability for tea beverages]

After tasting the tea beverages prepared based on HW, H3, M3, and M6 and the tea beverages of T1 to T8, 10 testers were asked to evaluate the aroma and taste to evaluate the preference on a scale of 1 to 10. After synthesizing and averaging the results, they are shown in [Table 7] and [Table 8] below. In the following indices, the higher the number, the better the degree of preference (rounding was applied to the average value for each index).

HW H3 M3 M6 palatability 3 One 3 One

(Unit: index)

T1 T2 T3 T4 T5 T6 T7 HW 3 7 8 3 7 7 6

(Unit: Index)

Referring to the above [Table 7], it was confirmed that the Aegis swimming extract had a very low palatability when applied as a beverage formulation due to its strong sour taste. In particular, in the case of H3 to which the fermentation process was applied, it was confirmed that the sour taste and the unique taste and flavor generated during the fermentation process lowered the overall palatability. In addition, it was confirmed that the same problem as above occurs in the mixed formulation of M3 and M6.

Accordingly, the mixed formulations of T1 to T7 were evaluated in order to provide a food formulation with excellent overall palatability by improving the flavor and taste as a tea beverage formulation based on natural plant extracts rather than artificial sweeteners. As a result, referring to [Table 8], in the case of T2 and T3 formulations in the mixing range, the unique sour taste of Baby Sooyoung is neutralized and as a premise, it can be seen that it can be produced as a beverage food having a highly palatable taste and aroma. there is. In particular, in the case of the range of the mixed formulation, not only M3 mixed with the hot water extract (HW) of A. It was confirmed that it can.

Therefore, in the case of the mixed formulation, it is known that the taste and flavor of the extract or the fermented product of the extract can be improved based on the mixed formulation based on natural plant extracts without using artificial sweeteners. Through this, not only can it be provided as a food formulation based on natural materials without problems such as side effects due to long-term intake and use, but also it can be provided and consumed more easily by consumers as excellent palatable food with excellent taste and flavor. You can check that you can.

Claims (7)

It contains the extract of Baby Suyeong,
Containing 1 to 5 parts by weight of lotus flower extract, 10 to 30 parts by weight of Aspergillus extract, 1 to 5 parts by weight of Noni extract, and 5 to 15 parts by weight of Chazuki extract, based on 100 parts by weight of the Baby Suyeong extract.
A composition for preventing and improving obesity. According to claim 1,
The extract is that the composition inhibits the expression of PPARγ and C / EBPα
A composition for preventing and improving obesity. According to claim 1,
The extract is extracted using an extraction solvent selected from the group consisting of water, C ₁ to C ₆ lower alcohols, and mixtures thereof.
A composition for preventing and improving obesity. According to claim 1,
The extract is Lactobacillus casei; Lactobacillus rhamnosus; Bifidobacterium bifidum Bifidobacteria; Bifidobacterium breve Bifidobacteria; Lactobacillus acidophilus; Any one selected from the group consisting of Bacillus subtilis and mixtures thereof is inoculated and fermented
A composition for preventing and improving obesity. delete A food for preventing and improving obesity, comprising the composition according to any one of claims 1 to 4. A functional food for preventing and improving obesity comprising the composition according to any one of claims 1 to 4.