KR20180026117A - Composition for preventing or treating obesity comprising red pepper seed’s extract - Google Patents

Abstract

The present invention relates to a pharmaceutical composition and a food composition for preventing or treating obesity comprising red pepper seed extract as an active ingredient. More particularly, the present invention relates to a pharmaceutical composition and a food composition for preventing or treating obesity comprising red pepper seed extract as an active ingredient in which the red pepper seed extract is soluble in hot water and is spray-dried. The method of the present invention can be effectively used for prevention or treatment of obesity since the extract of pepper seed shows an effect of inhibiting adipocyte differentiation.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing or treating obesity comprising extract of red pepper seeds as an active ingredient.

The present invention relates to a composition for preventing or treating obesity comprising red pepper seed extract as an active ingredient, and more particularly, to a composition for preventing or treating obesity, The present invention relates to a pharmaceutical composition and a food composition for prevention or treatment of obesity, which comprises an extract of pepper seeds as an active ingredient.

Obesity is not a simple weight gain or appearance problem but a condition that can cause serious diseases. It causes metabolic diseases such as sleep apnea, osteoarthritis and hypertension, diabetes, dyslipidemia and metabolic syndrome and eventually causes cardiovascular diseases and stroke It can lead to a fatal cessation that leads to death. It is a symptom that the world is paying attention to the threat of obesity as WHO points out obesity as a new infectious disease in the 21st century. WHO estimates that over one billion people worldwide are obese and that heart disease caused by overweight and obesity is the world's leading death toll, and that by 2015 about 23.4% of the world's population is obese And obesity is expected to increase by 50% over the next 10 years. Obesity is a rapidly growing worldwide prevalence.

 In Korea, the prevalence of obesity has increased rapidly from 26.3% in 1998 to 29.6% in 2001 and 31.8% in 2005, compared to the 1998 and 2001 and 2005 National Health and Nutrition Surveys. The prevalence of obesity in adolescents is increasing year by year, and many obesity in adolescents leads to adult obesity, leading to various diseases such as chronic diseases, cancer, and diabetes. In the case of young adults, the four major health problems (cancer, brain disease, cardiovascular disease, rare disease) are associated with obesity-related insulin resistance, accounting for about 50% of Koreans' deaths.

Obesity is caused by storing excess calories in the body in the form of fat. As a result of economic development and technological advances, consumption of fat and sugar increases, and dietary and lifestyle changes, which decrease fiber intake, The proportion of the obese population is gradually increasing due to the decrease in metabolism relative to intake. Obesity is explosive in the same generation, so dieting, exercise or lifestyle changes alone can not solve it. It is well known that anti-obesity drugs that are distributed on the market show many side effects and show a great deal of societal severity. Accordingly, researches for developing anti-obesity raw materials using natural substances having high safety with few side effects such as plant extracts other than the chemical synthetic products have been actively conducted.

As the major causes of obesity are found in dietary habits and the adverse effects of abuse of medicines are widely recognized, there is a growing interest in functional foods as an approach to health promotion or disease prevention. In addition to the nutritional and symbolic functions (sensory functions) that maintain the life and health of food, the third functions include bio-defense, regulation of biorhythm, prevention of obesity, prevention of aging, The development and release of various functional foods with a focus on functions are also lively. In particular, recent research results have revealed that foods using natural products are supplemented with biohazard systems for diseases by offsetting the effects of environmental hormones incorporated or remaining in food or the surrounding environment, or by activating or metabolizing the immune system Industrialization of functional foods or medicinal foods using herbal medicine resources has been attracting attention as a burden.

Of the anti-obesity materials that utilize natural materials, capsaicin of pepper is known to suppress the fat accumulation and to exhibit the effect of anti-obesity. In addition, capsaicin is the main component of the pungent taste of pepper. It is a major component of the pungent taste of pepper. It regulates humoral immune function and regulates cancer gene expression and anti-adulthood, functional dyspepsia, energy metabolism, sterilization, induction of apoptosis and inhibition of fat production in 3T3- And the effect of other factors. It also contains phenolic compounds such as quarcetin, capsaicinoids and luteolin, which are being studied in various fields. Studies on the composition of red pepper seed oil, the purification and analysis of red pepper seed oil, and the storage stability of red pepper seed oil have been carried out in recent years. However, recent studies on antimutagenic effect, anticancer effect, , And chemical composition analysis studies are progressing gradually.

Accordingly, the present inventors propose a new use of pepper seed extract and provide a pharmaceutical composition and a functional food composition containing an active ingredient of a hot-water extract of a pepper seed extract as an effective ingredient to prevent the adipocyte differentiation and the fat synthesis, The present invention has been completed in order to alleviate and improve symptoms.

Accordingly, an object of the present invention is to provide a pharmaceutical composition for preventing or treating obesity, which comprises Red Pepper Seed's Extract as an active ingredient.

Another object of the present invention is to provide a food composition for preventing or ameliorating obesity, which comprises pepper seed extract as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing or treating obesity, which comprises extract of red pepper seeds as an active ingredient.

In order to accomplish another object of the present invention, the present invention provides a food composition for prevention or improvement of obesity comprising an extract of pepper seed as an active ingredient.

Hereinafter, the present invention will be described in detail.

The composition of the present invention is characterized by having an effect of preventing, ameliorating and treating obesity, comprising an extract of red pepper seeds as an active ingredient.

The red pepper used in the present invention is the fruit of the red pepper (Capsicum annuum L.) or its varieties belonging to the perennial herbs of the branches and is the main spice originating in South America and widely used in the Southeast Asian region including Korea now. In the present invention, pepper represents the part of the red pepper fruit. The red pepper fruit is a long, rounded shape, initially dark green, gradually reddish as it ripens, and the skin and seeds have a very pungent flavor and contain a lot of capsaicin.

The method of extracting hot pepper seeds of the present invention can be carried out by a solvent extraction method known in the art, but it can be preferably extracted by hot water extraction. The extract of red pepper seeds extracted with water can be used by freeze drying or spray drying. In one embodiment of the present invention, hot pepper seeds are extracted with hot water, followed by lyophilization or spray drying to obtain a final extract powder. As a result of measuring the inhibitory effect on the differentiation of adipocyte, the extract of the present invention showed a high inhibitory effect on adipocyte differentiation (see Example 1).

It is preferable that the extract of hot pepper seeds is an extract which is soluble in hot water, and both freeze-drying and spray-drying are effective for anti-obesity effect, but spray-dried extract is more preferable. The extract of hot pepper seeds was obtained by hot extraction of dried red pepper seeds and spray drying to obtain a final extract powder. In another embodiment of the present invention, when the extracted extract was dried by freeze-drying and spray drying, spray-dried capsicum extract showed a high inhibitory effect on differentiation of adipocytes (see Example 2).

In the present invention, obesity refers to a state in which fat is excessively accumulated in the body due to an increase in size or number of fat cells. Adipose tissue is an energy-storing organ. Excess energy intake promotes the differentiation of adipocytes and increases the amount of stored fat in the body, which is a direct cause of obesity. The adipocyte differentiation refers to a process in which a preadipocyte is transformed into a mature adipocyte through proliferation and differentiation. In this process, the fatty acid synthase (FAS) involved in lipogenesis and the ACC (acetyl-CoA carboxylase). These gene expressions are regulated by transcription factors such as peroxisome proliferator-activated receptor gamma (PPARgamma), C / EBPs (CCAAT / enhancer binding proteins), and SREBP1c (sterol regulatory element binding protein 1c). These transcription factors induce adipocyte differentiation during differentiation of adipocytes and induce adipocyte differentiation by controlling the expression of various adipocyte-specific genes through mutual interaction (J. Agri. Food Chem. 4): 1094-1101, 2012, Nutr. Metab. 6 (20): 1-10, 2009).

The pharmaceutical composition for prevention and treatment of obesity including the extract of red pepper seeds of the present invention may further comprise suitable carriers, excipients and diluents conventionally used for the production of pharmaceutical compositions. The composition containing the compound according to the present invention may be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups and aerosols, external preparations, suppositories and sterilized injection solutions, Examples of carriers, excipients and diluents that can be contained in the composition including the compound include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate,

Calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, Or lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate talc are also used. Examples of the liquid preparation for oral administration include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the composition of the present invention varies depending on the condition and the weight of the patient, the degree of disease, the type of drug, the route of administration and the period of time, but can be appropriately selected by those skilled in the art.

The composition of the present invention may be administered to mammals such as rats, mice, livestock, humans, and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection.

The present invention also provides a food composition for preventing or ameliorating obesity, which comprises an extract of pepper seed as an active ingredient.

The pepper seed extract can be applied to various forms of food for prevention and improvement of obesity and weight loss. At this time, the hot pepper seed extract of the present invention can be directly added to the food, can be used together with other foods or food ingredients, and can be added to functional foods and health supplements, which can be suitably used according to conventional methods.

The amount of the active ingredient mixed as the food can be suitably determined according to the purpose of use (prevention, health or therapeutic treatment). In general, the complex extract of the present invention in the production of food or beverage can be appropriately adjusted in the range of 0.0001 to 99.9% in the whole food composition. However, in the case of long-term ingestion intended for health and hygiene purposes or for health control purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

There is no particular limitation on the kind of the food, and it includes food, functional food and health supplement food in a conventional sense. For example, when it is applied as a beverage, various flavors or natural carbohydrates may be added as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like.

As the food, the raw materials can be divided into the stocks / cores, the sub-materials, and the favorite foods as follows, but the present invention is not limited thereto. These foods further contain the extract of pepper seeds of the present invention to induce an anti-obesity effect. Stocks / corroded products include milk / processed oil, lactose-degraded milk, fermented milk, butter oil, milk cream, buttermilk, cheeses and milk products; Meat products such as processed meat products, egg products, hamburgers, etc; Fish meat products such as fish cakes, ham, sausage, bacon and the like; tofu / mugwort such as tofu and mugwort; Noodles such as noodles, noodles, noodles, fresh noodles, noodles, luxury noodles, improved noodles, frozen noodles, pastas; Kimchi, pickles, salted fish, pickles, and the like. Ingredients include sauces such as soy sauce, miso, kochujang, spring rolls, chonggukjang, mixed berries, vinegar, sauce, tomato ketchup, curry, dressing; Soybean oil, rice bran oil, margarine and shortening.

Also, preference foods include leaching / leaching tea, powder tea, concentrate tea and coffee; Beverages such as fruit drinks, vegetable drinks, carbonated drinks, two-oil drinks, yogurt, and beverages such as mixed drinks; Confectionery such as bread, rice cake, dried fruit, candy, chocolate, chewing gum, and mackerel; Ice cream products such as ice cream, ice cream, and ice cream powder. It can be used for various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, Carbonating agents, and the like.

In addition, the complex extract of the present invention may contain flesh for the production of natural fruit juice, fruit juice beverage and vegetable beverage. 'Functional food' means foods designed and processed so that the biomechanical functions such as bio-defense, regulation of biorhythm, prevention and recovery of disease, etc. of food components can be fully exerted in living bodies. Examples of the functional food include a hypertensive food and a cholesterol-controlled food. The functional food includes the extract of the present invention. The functional food further induces various functions of the functional food, as well as inhibition and control of obesity. The extracts exhibit functionalities such as weight reduction, abdominal obesity reduction, waist circumference reduction, and body fat reduction through anti-obesity, more specifically, the cellular delivery system involved in body fat reduction such as PPAR-γ and C / EBP-α.

'Health supplement food' refers to foods prepared by extracting, concentrating, mixing, etc. a specific ingredient contained in a food ingredient for the purpose of supplying a characteristic ingredient to be applied to a human body in nutrition or physiology.

The health supplements include eel processed foods, royal jelly processed foods, yeast foods, pollen processed foods, squalane foods, enzyme foods, lactic acid bacteria foods, chlorella foods, gamma linolenic acid foods, embryo processed foods, soybean lecithin processed foods, octacosanol foods, Grape seed oil food, plant extract fermented food, aloe food and chitosan processed foods, and the combined extract of the present invention is further added to these health supplements to induce the effect of the food and the suppression and control effect of obesity. The above ingredients can be used independently or in combination. The ratio of the additive is not critical. However, the extract of the present invention is generally selected in the range of 0.0001 to 99.9% based on the total composition.

Accordingly, the present invention provides a pharmaceutical composition and a food composition for preventing or treating obesity comprising Red Pepper Seed's Extract as an active ingredient. Since the method of the present invention shows inhibition of adipocyte differentiation and lipid synthesis inhibition of pepper seed extract, weight loss, body fat reduction, and abdominal fat through PPAR-γ and C / EBP- And a reduction in waist circumference can be expected, so that it can be usefully used for prevention or treatment of obesity.

FIG. 1 shows extraction conditions of the extract of red pepper seeds according to the present invention, and shows the effect of inhibiting lipid differentiation from the 3T3-L1 cell line according to each condition.
Fig. 2 shows the result of lipid production using Oil Red O staining reagent in the same cell line. Cell extracts were treated with 50 [deg.] C hot water extraction followed by lyophilization and fractional drying, Inhibition of lipid synthesis.
FIG. 3 shows the degree of lipid synthesis by oil red O staining in the same cells as above using an extract dried by spray drying after hot water extraction.

Hereinafter, the present invention will be described in detail.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

<Experimental Method>

1. Preparation of red pepper seeds extract using water solvent

Dried red pepper seeds received from Nutritional Pepper Distribution Corporation were purchased and used in the experiment. Extraction was carried out under three different extraction conditions using the red pepper seeds.

First, after roughening the red pepper seeds, the ratio of red pepper seeds and water was 1: 9, and then mixed at 4 ° C for 24 hours. After the mixture was centrifuged, the supernatant was collected and centrifuged again. The supernatant was filtered through a 90 mm / pore size 5 탆 filter, concentrated and lyophilized to obtain a final extract powder.

Second, circulating extraction was performed for 22 hours or more at a temperature of 50 ° C or more by adding 1,800 L of water, which was 9 times, to 200 kg of roughly crushed chilli. Thereafter, the mixture was cooled and separated for more than 12 hours, and then centrifuged to obtain an extract concentrate.

Two extractive powders were obtained at the end using two drying methods with this extract. Half of the above concentration was freeze-dried to produce powder having a final water content of 50% or less, and then sieved to a size of 50 to 100 mesh to obtain a uniform powdery extract. The other half of the concentrate was dried to obtain a powder having a final moisture content of 5% or less, and then sieved to a size of 50 to 100 mesh to obtain an extract powder having a uniform size.

2. Inhibition effect of lipid differentiation according to temperature difference under the same extraction condition

All of the conditions were extracted with water, and the extracts obtained by lyophilization were divided into 50 ° C and 4 ° C in the intermediate process. The experiment was divided into three groups. C was distilled water (water), RPS50 was hot water extract of hot pepper seeds (50 ℃) and RPS4 was hot pepper seed extract (4 ℃) In the same way, we tried to find out whether the effect of water extraction and which method is more effective when the temperature is different. Based on this, it was used as a basis for judging whether it is more suitable for mass production in the future. More specifically, by adding a reagent that selectively stains lipids such as Oil Red O to red, the 3T3-L1 cell line synthesizes lipids dependently upon differentiation, so the degree of staining is checked when the above three substances are added , And the degree of dilution of the stain is inhibited.

3. Effect of drying method on inhibition of lipidation

Oil Red O staining reagent was used for the same cell line used in the above experiment. The cell extract-treated extracts were treated with 50 째 C hot water, followed by lyophilization and drying to obtain cells, , And the degree of inhibition of lipid synthesis. The experiment was divided into three groups. C was distilled water (water), RPSF-100 was freeze-dried extract, and RPSS-100 was spray-dried extract.

4. Inhibitory effects of extracts on lipid differentiation

 The RPSS-50 was spray dried extract (50 ug / ml), RPSS-100 was spray dried extract (100 ug / ml), and the RPSS- RPSS-200 showed a concentration of spray-dried extract of 200 ug / ml. Each extract was treated with the same cell line as described above, and the degree of fat synthesis was evaluated through Oil Red O staining.

&Lt; Example 1 >

Identification of adipocyte differentiation and lipid accumulation inhibitory effect of the prepared red pepper seed extract

Using 3T3-L1 cell line as an extract according to each extraction condition, we examined which extracts were effective in inhibiting adipocyte differentiation and inhibiting adipogenesis. First, the 3T3-L1 cell line is a cell line derived from a mouse, and is a widely used cell line when studying the mechanism such as differentiation of adipocytes and fat production. The differentiation of cells mainly increased the synthesis and accumulation of triglycerides. This cell line was maintained in DMEM containing 10% calf serum. When confluent phase became possible, the differentiation inducer was treated to induce differentiation. The material used may be one of IBMX, Dexa, Insulin, (MDI) and FBS. In order to determine the effect of the lyophilized extract powder extracted at 4 ° C and 50 ° C on cell differentiation, As a confirmation method, the degree of differentiation of adipocytes was confirmed by confirming the degree of staining using Oil Red O staining reagent which specifically stains lipid.

As a result, the inhibition pattern of adipocyte differentiation was confirmed in all the extracts obtained at 4 ° C and 50 ° C. And the extract obtained at 50 DEG C compared to 4 DEG C showed a more effective inhibitory pattern (Fig. 1).

&Lt; Example 2 >

Identification of adipocyte differentiation and inhibition of fat accumulation of pepper seed extract according to drying method and concentration

The degree of suppression of fat differentiation was observed with the lyophilized extract at 50 ° C. and the spray dried extract obtained at the same temperature of 50 ° C., which proved the effect through the above experiment. As a result, both of the extracts showed an inhibitory effect on lipid differentiation, and the spray-dried extract showed a greater inhibitory effect than the lyophilized extract (FIG. 2).

Based on the above results, the cells were treated with 50, 100, and 200 ug / ml extracts of spray dried extracts at 50 ℃, respectively. The degree of staining of Oil Red O staining reagent was also confirmed. As a result, the fat accumulation inhibitory effect was shown at all concentrations, and the inhibitory effect was confirmed in a concentration-dependent manner in proportion to the treatment concentration (FIG. 3).

<Formulation example>

1. Preparation of tablets

Hot water extract of red pepper seeds of Example 1. 100 mg

Starch ................................................. ...... 50 mg

Lactose ................................................. ...... 50 mg

Magnesium stearate ........................... 5mg

The above components were mixed and tablets were prepared by a wet granulation method or a dry granulation method according to a conventional method for producing tablets.

2. Preparation of capsules

Hot water extract of red pepper seeds of Example 1. 100 mg

Starch ................................................. ...... 50 mg

Lactose ................................................. ...... 50 mg

Talc ................................................. ...... 1 mg

Magnesium stearate ............................. 5mg

The above components were mixed and capsules were prepared according to the conventional preparation method of capsules.

3. Manufacture of liquid agent

Hot water extract of red pepper seeds of Example 1. 500 mg

Isolation Party ................................................ ..10g

saccharose................................................. ........ 10g

Lemon flavor ................................................ ..... Appropriate amount

Purified water was added to the whole .............................. 50 ml

4. Manufacture of beverages

Example 1: Hot water extract of red pepper seeds ... 100 mg

Vitamin B2 ................................................ .... 5 mg

Vitamin B6 ................................................ .... 5 mg

Vitamin C ............................................ .... ... 80 mg

Dietary fiber .............................................. 1000㎎

Liquid fructose ............................................ 10,000㎎

Emulsifier ................................................. ... 100 mg

Lemon flavor ................................................ ...... 50 mg

Purified water................................................. .... Appropriate amount

The above components were mixed to make a volume of 50 ml. The mixture was passed through a 3-μm filter, and the resultant filtered mixture was first sterilized at 90 to 93 ° C for 20 seconds, filled in a 50 ml bottle, heated at 80 to 85 ° C for 20 minutes, The beverage products were prepared by sterilization.

Having described specific portions of the present invention in detail, those skilled in the art will appreciate that these specific descriptions are only for the preferred embodiments, and the scope of the present invention is not limited thereto.

The present invention relates to a composition for preventing or treating obesity comprising red pepper seed extract as an active ingredient and more particularly to a composition for prevention or treatment of obesity containing an active ingredient, Prevention or improvement of the food composition. The extract of the present invention showed a higher effect of inhibiting the adipocyte from spray drying than that of freeze-drying, and showed a high effect on the effect of inhibiting adipocyte when administered at a high concentration of the extract of the present invention. Therefore, And thus is highly industrially applicable.

Claims (9)

A pharmaceutical composition for preventing or treating obesity comprising Red Pepper Seed's Extract as an active ingredient.
The pharmaceutical composition according to claim 1, wherein the hot pepper seed extract is a hot-water extract.
The pharmaceutical composition according to claim 1, wherein the obesity is any one selected from the group consisting of overweight, upper and lower abdominal obesity, thigh circumference obesity, and hip circumference obesity.
The pharmaceutical composition according to claim 1, wherein the pharmaceutical composition is an oral formulation and has any one of formulations selected from the group consisting of tablets, capsules, pills, granules, powders, extracts, suspensions, emulsions, syrups and aerosols &Lt; / RTI &gt;
A food composition for preventing or ameliorating obesity comprising Red Pepper Seed's Extract as an active ingredient.
The food composition according to claim 5, wherein the food is a functional food.
6. The food composition according to claim 5, wherein the pepper seed extract is an extract which is soluble in hot water.
The food composition according to claim 5, wherein the obesity is any one selected from the group consisting of overweight, upper and lower abdominal obesity, thigh circumference obesity, and hip circumference obesity.
The food according to claim 5, characterized in that the food has any one property selected from the group consisting of powder, granule, tablet, capsule, syrup, beverage, chewing gum, tea, candy, caramel, beverage, Composition.

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