KR20180010630A - Increasing method of the gaba component in seed - Google Patents
Increasing method of the gaba component in seed Download PDFInfo
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- KR20180010630A KR20180010630A KR1020160093066A KR20160093066A KR20180010630A KR 20180010630 A KR20180010630 A KR 20180010630A KR 1020160093066 A KR1020160093066 A KR 1020160093066A KR 20160093066 A KR20160093066 A KR 20160093066A KR 20180010630 A KR20180010630 A KR 20180010630A
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- plasma
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
Abstract
Description
The present invention relates to a method for enhancing GABA (gamma-aminobutyric acid) content of seeds, and more particularly, to a method for enhancing GABA content of seeds using plasma.
GABA is an abbreviation for gamma-aminobutyric acid. GABA, together with glutamic acid and glycine, is one of the most commonly used amino acid neurotransmitters in the central nervous system of mammals. GABA is contained in many cereals such as human brain, vegetables, fruit, rice and brown rice. It improves cerebral blood flow, increases oxygen supply, stimulates brain cell metabolism function, so it can relieve stress, relieve stress, improve memory, , Depression relief, stroke and dementia prevention, insomnia, obesity, and menopause is said to be effective. In particular, recently, it has been found that GABA has an effect of inhibiting the metastasis and proliferation of stroke, colon cancer, and colon cancer cells.
GABA in plants is known to play an important role in growth and self-protection through activation of plant cells, such as increasing pest attack and seed germination. In other words, it is presumed that the GABA generation system is activated as one of the measures against various environmental stresses. Therefore, the content of GABA may increase due to environmental changes such as germination or anaerobic treatment, rapid temperature change, and mechanical stimulation.
Therefore, studies to improve the content of GABA by using these characteristics are continuing, but until now, it takes a lot of time and cost to increase the GABA content, and the degree of increase of GABA content is insufficient. There is a need for further development of a method which can economically and easily enhance the GABA content.
It is an object of the present invention to provide a method for increasing the GABA content of a seed using plasma discharge water.
It is another object of the present invention to provide a method for enhancing the GABA content of a seed using a plasma surface treatment.
A method for enhancing the gamma-aminobutyric acid (GABA) content of seeds for one purpose of the present invention comprises immersing germable seeds in a plasma discharge water.
In one embodiment, the plasma discharge water may have a pH of 5 or less.
In one embodiment, the plasma discharge water may be a plasma discharge number generated by an underwater plasma discharge or a water surface plasma discharge.
In one embodiment, the step of pre-treating the surface of the germable seed with plasma prior to the step of dipping in the plasma discharge water. At this time, the plasma discharge water may have a pH of 6 or less.
A method for enhancing the GABA content of seeds for another purpose of the present invention comprises surface treating the germable seeds in a dry state with a plasma.
In one embodiment, after the surface treatment with the plasma, the step of immersing the germable seed surface-treated with the plasma in the plasma discharge water may be included. At this time, the plasma discharge water may have a pH of 6 or less. At this time, the plasma discharge water may be a plasma discharge water generated by an underwater plasma discharge or a water surface plasma discharge.
In one embodiment, the plasma may be a DBD atmospheric plasma.
According to the seed GABA content increasing method of the present invention, the GABA content of germable seeds can be enhanced by using plasma. According to the present invention, the germable seed surface is treated with a plasma Direct or indirect By treating or treating with plasma discharge water, it is possible to increase the GABA content of germable seed quickly and easily. By increasing the GABA content quickly and easily, manufacturing time and cost can be saved, and a high content of GABA can be obtained economically. In addition, by treating the surface of the germable seed with a plasma, the seed surface can be sterilized by removing the harmful components of the seed surface together with the enhancement of the GABA content, and the seed surface can be modified to promote seed germination and growth have.
1 is a view for explaining the enhancement of GABA content of seeds according to Example 1 of the present invention.
2 is a view for explaining the GABA content enhancement of a seed according to Example 2 of the present invention.
3 is a view for explaining the enhancement of the GABA content of the seed according to Example 3 of the present invention.
Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention is capable of various modifications and various forms, and specific embodiments are illustrated in the drawings and described in detail in the text. It is to be understood, however, that the invention is not intended to be limited to the particular forms disclosed, but on the contrary, is intended to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. Like reference numerals are used for like elements in describing each drawing.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In the present application, the term "comprises" or "having ", etc. is intended to specify that there is a feature, step, operation, element, part or combination thereof described in the specification, , &Quot; an ", " an ", " an "
Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in commonly used dictionaries are to be interpreted as having a meaning consistent with the contextual meaning of the related art and are to be interpreted as either ideal or overly formal in the sense of the present application Do not.
The method of enhancing the GABA (gamma-aminobutyric aicd) content of seeds of the present invention can use plasma as a metabolic stimulation source of germable seeds for promoting GABA content. As used herein, the term " metabolic stimulation source " means a stimulation source capable of stimulating metabolism, which is a chemical change related to decomposition and synthesis of substances in vivo. The present invention uses plasma as a metabolic stimulus source to treat germable seeds Whereby the GABA component in the germable seeds can be promoted.
In the present invention, "germable seeds" may include embryos (seeds) and may mean germable seeds or seeds. As an example, the germable seeds of the present invention may be cereal seeds, pulp seeds. Grains include rice, wheat, barley, corn, and other beans; beans such as beans, beans, mung beans, kidney beans, peas, and peanuts. As an example, the seeds of the present invention may be rice, preferably brown rice.
GABA, gamma-aminobutyric acid is an inhibitory amino acid neurotransmitter, a natural component that helps suppress blood pressure, relieve stress, and stimulate memory learning. GABA is contained in human brain, vegetables, fruits, grains, and the like.
In the present invention, plasma treatment may mean plasma treatment of germable seeds, using plasma discharge water produced by treating plasma with water, or treating the plasma directly with germable seeds or indirect treatment. At this time, the plasma may be a dielectric barrier discharge (DBD) atmospheric plasma.
For example, the method for enhancing the GABA content of a seed using the plasma discharge water of the present invention includes a step of immersing germable seeds in a plasma discharge water. At this time, the plasma discharge water may have a pH of 5 or less.
The plasma discharge water means water subjected to a plasma discharge treatment, and the plasma discharge water may be a plasma discharge water generated by an underwater plasma discharge or a water surface plasma discharge. For example, the present invention can use the plasma discharge water generated by the water surface plasma discharge. The plasma discharge water may contain a large number of active gas species such as reactive nitrogen species and reactive oxygen species.
According to the present invention, in contrast to conventionally treating a seed for a long time by using a GABA generator to increase the GABA content, the present invention is characterized in that the germable seed is treated with the plasma discharge water for a short time, GABA content can be increased. In addition, the GABA content can be effectively increased even in a short time. That is, it is possible to show a degree of enhancement of the content of GABA that is superior to the conventional one, and thus it is possible to provide the seed having the high content of GABA according to the invention. For example, when the germable seed is plasma-treated using the plasma discharge water, the germable seed may exhibit an excellent GABA content even when it is immersed in the plasma discharge water for 3 hours.
The germable seed may be pretreated with plasma before being dipped in the plasma discharge water. For example, germable seeds pretreated with a plasma can be dipped into plasma discharge water having a pH of 6 or less.
Alternatively, the present invention may increase the GABA content of the seed by direct or indirect treatment of the germable seed with a plasma. Direct plasma treatment may mean directly treating the plasma, and plasma indirect treatment may mean using radicals generated in the plasma or active gas species such as active nitrogen species and reactive oxygen species. The present invention may also enhance the GABA content of germable seeds by treating the germable seed surface through plasma direct or indirect treatment.
Specifically, the method for enhancing the GABA content of a seed using the plasma treatment of the present invention includes a step of surface-treating germable seeds in a dry state with a plasma.
In one example, the plasma surface treatment may be to treat the germable seed surface for 1 to 24 minutes in a dry state.
The germable seeds surface-treated with the plasma may further include a step of immersing the seed in the plasma discharge water. At this time, the pH of the plasma discharge water may be lower than the
By treating the germable seed with the plasma according to the present invention, the content of GABA contained in the embryo of the seed can be enhanced by the present invention. Specifically, GABA production is mainly caused by the action of glutamate decarboxylase (GAD), which converts glutamate into GABA. When the environment is acidified, the activity of GAD involved in GABA formation is increased, but the activity of GABA NH Gamma-aminobutyric acid (γ-aminobutyrate: pyruvate transaminase), which is involved in the second stage of metastasis, is inhibited and consequently accumulates GABA. The present invention can enhance the GABA content in the seed by oxidizing the seed through plasma treatment. In addition, by germinating the cereal seed, a high content of GABA in the embryo of the cereal seed is transferred to the endosperm to obtain cereals showing a high GABA content.
Further, the present invention can improve the GABA content of the germable seed by treating the germable seed using plasma, and at the same time, it is possible to remove harmful components from the seed surface. In addition, through the surface modification of the seed, germination and growth of the seed may be promoted.
Hereinafter, a method of enhancing the GABA content of seeds of the present invention will be described with reference to specific examples.
Example 1: Enhancement of seed GABA content by direct plasma treatment
5 g of germable brown rice containing the seeds was directly surface treated with DBD atmospheric plasma for 3 minutes. The plasma treated brown rice was then germinated and then the GABA content of the brown rice was calculated as the GABA content per 100 g. As a control group, the GABA content per 100 g of 5 g of brown rice (hereinafter, referred to as Comparative Example 1) without any treatment was calculated. The results are shown in FIG. 1 and Table 1.
1 is a view for explaining the enhancement of GABA content of seeds according to Example 1 of the present invention.
1, the GABA content per 100 g of the surface treated with the plasma according to Example 1 of the present invention was 33.38 mg, and the GABA content according to Comparative Example 1 was 4.91 mg, 7-fold increase.
Thus, according to Example 1 of the present invention, it can be confirmed that the GABA content of germable seeds can be enhanced only by plasma direct surface treatment.
Example 2: Enhancement of seed GABA content using plasma discharge water
First, DBD atmospheric plasma was used to discharge the water surface to produce plasma discharge water. The plasma discharge water was diluted to pH 2.47, pH 3.10 and pH 4.10, respectively, and 5 g of germable brown rice containing cilium was immersed in each plasma discharge water for 3 hours. After germination, the GABA content of brown rice was calculated as the GABA content per 100 g. In addition, the GABA content per 100 g of 5 g of brown rice treated with distilled water (hereinafter referred to as Comparative Example 2) was calculated and compared with the GABA content of brown rice according to Example 2 of the present invention. The detailed conditions and thus the GABA content of the brown rice are shown in the following Table 2, and the graph according to the GABA content is shown in Fig.
2 is a view for explaining the GABA content enhancement of a seed according to Example 2 of the present invention.
Referring to FIG. 2 and Table 2 together, as shown in Example 1, the brown rice without any treatment of Comparative Example 1 had a GABA content of 4.91 mg per 100 g, and the brown rice GABA The content of GABA in the brown rice according to Example 2 (Examples 2-1 to 2-3) of the present invention was about 45 mg to 60 mg, which was 9.21 mg in comparison with Comparative Examples 1 and 2 Indicating higher GABA content. That is, it can be confirmed that the GABA content of the brown rice is increased even in a short-time treatment of 3 hours, and thus it can be confirmed that the GABA content of the seed can be easily and quickly improved according to the present invention.
In addition, it can be confirmed that the GABA content of the brown rice immersed in the plasma discharge water having a pH of 5 or less is enhanced, and that the highest GABA content is shown when the pH of the plasma discharge water is
Therefore, according to the present invention, it can be confirmed that the germable seeds can be immersed in the plasma discharge water for a short time to enhance the content of GABA in the seeds.
Example 3: Enhancement of seed GABA content using plasma direct treatment and plasma discharge water
5 g of germable brown rice containing the seeds was directly surface treated with DBD atmospheric plasma for 3 minutes. Next, DBD atmospheric plasma was discharged at the surface of the water to prepare plasma discharge water at various pH conditions of pH 2.47, pH 3.10, pH 4.10, pH 5.00, and 5 g of brown rice directly surface- Water for 3 hours. After germination, the GABA content of brown rice was calculated as the GABA content per 100 g. The detailed conditions and thus the GABA content of the brown rice are shown in the following Table 3, and the GABA content graph is shown in Fig.
3 is a view for explaining the enhancement of the GABA content of the seed according to Example 3 of the present invention.
Referring to FIG. 3 together with Table 3, Example 3 of the present invention (Examples 3-1 to 3-5) in which the germable brown rice was directly surface-treated with plasma, and then the brown rice surface-treated with plasma was immersed in plasma discharge water. The GABA content of the brown rice according to Example 3-4) is higher than that of Comparative Example 1 in which no treatment is applied to the brown rice and Comparative Example 2 treated with the plasma discharge water.
Specifically, it can be confirmed that the pH of the plasma discharge water after the surface treatment with plasma shows an excellent GABA content in the range of
Therefore, it can be confirmed that the present invention can easily and effectively enhance the GABA content of brown rice.
In general, as has been confirmed in Examples 1 to 3 above, according to the present invention, it is possible to easily and quickly increase the GABA content of seeds by directly treating the germable seeds with plasma or using plasma discharge water . Further, it can be confirmed that the seeds can exhibit a better GABA content by treating the germable seeds with the plasma discharge water after the plasma direct surface treatment.
It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention as defined by the following claims. It can be understood that it is possible.
Claims (10)
Methods for enhancing GABA (gamma-aminobutyric acid) content of seeds.
Wherein the plasma discharge water has a pH of 5 or less.
Methods for enhancing the GABA content of seeds.
Wherein the plasma discharge water is a plasma discharge water generated by an underwater plasma discharge or a water surface plasma discharge.
Methods for enhancing the GABA content of seeds.
Before the step of immersing in the plasma discharge water,
Treating the surface of the germable seed with a plasma.
Methods for enhancing the GABA content of seeds.
Characterized in that the plasma discharge water has a pH of 6 or less.
Methods for enhancing the GABA content of seeds.
Methods for enhancing the GABA content of seeds.
After the surface treatment with the plasma,
And dipping the germable seed surface-treated with the plasma into the plasma discharge water.
Methods for enhancing the GABA content of seeds.
Characterized in that the plasma discharge water has a pH of 6 or less.
Methods for enhancing the GABA content of seeds.
Wherein the plasma discharge water is a plasma discharge water generated by an underwater plasma discharge or a water surface plasma discharge.
Methods for enhancing the GABA content of seeds.
Wherein the plasma is a dielectric barrier discharge (DBD) atmospheric plasma.
Methods for enhancing the GABA content of seeds.
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| Application Number | Priority Date | Filing Date | Title |
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| KR1020160093066A KR101963935B1 (en) | 2016-07-22 | 2016-07-22 | Increasing method of the gaba component in seed |
| PCT/KR2017/007752 WO2018016862A1 (en) | 2016-07-22 | 2017-07-19 | Method for increasing gaba content of seed |
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| KR1020160093066A KR101963935B1 (en) | 2016-07-22 | 2016-07-22 | Increasing method of the gaba component in seed |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102332152B1 (en) * | 2021-04-08 | 2021-12-01 | 한국핵융합에너지연구원 | The composition for aeroponic culture system containing non-thermal plasma treated water |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102400706B1 (en) * | 2019-12-12 | 2022-05-24 | 대한민국 | Method for increasing active ingredient of barley sprout using plasma treatment |
| CN111700200A (en) * | 2020-06-18 | 2020-09-25 | 南京农业大学 | Barley seedling powder rich in phenolic acid and production process thereof |
| KR102559546B1 (en) * | 2020-09-01 | 2023-07-27 | 대한민국 | Method for enhancing the content of functional ingredients in seeds |
| KR102254560B1 (en) | 2020-12-08 | 2021-05-21 | (주)안온바이오 | Extract of Liriope platyphylla with increased gaba content and anti-inflammatory composition comprising the same |
| KR20220159205A (en) | 2021-05-25 | 2022-12-02 | 재단법인 철원플라즈마 산업기술연구원 | Plasma complex microbubble fused water production system |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10276597A (en) * | 1997-04-09 | 1998-10-20 | Ryoda Sato | Solution culture system using active water and raising system |
| KR20170056067A (en) * | 2015-11-12 | 2017-05-23 | 한국식품연구원 | Method of Cultivating Soybean Sprouts using Water treated with Plasma |
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| KR101294252B1 (en) * | 2011-12-29 | 2013-08-08 | 김문겸 | Water cultivation apparatus using plasma |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10276597A (en) * | 1997-04-09 | 1998-10-20 | Ryoda Sato | Solution culture system using active water and raising system |
| KR20170056067A (en) * | 2015-11-12 | 2017-05-23 | 한국식품연구원 | Method of Cultivating Soybean Sprouts using Water treated with Plasma |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102332152B1 (en) * | 2021-04-08 | 2021-12-01 | 한국핵융합에너지연구원 | The composition for aeroponic culture system containing non-thermal plasma treated water |
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| WO2018016862A1 (en) | 2018-01-25 |
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