KR20180005387A - Cosmetic Composition For A Alleviating Atopic Dermatitis And Pruritis Using By Bioconversion Of The Extracts and Method For Manufacturing the same - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating atopic dermatitis and pruritus and, more specifically, to a cosmetic composition for alleviating atopic dermatitis and pruritus comprising bioconversion extracts obtained through bioconversion of natural extracts which are safe to the human body to alleviate pruritus caused by atopic dermatitis, and to a method for manufacturing the same.

Description

TECHNICAL FIELD The present invention relates to a cosmetic composition for improving atopic pruritus including a biotransformant extract and a method for producing the same.

The present invention relates to a cosmetic composition for improving atopic pruritus. More specifically, the present invention relates to a cosmetic composition for improving atopic pruritus including a biotransformation extract obtained by biotransformation of a natural extract safe for human body in order to alleviate pruritus caused by atopic dermatitis To a cosmetic composition and a method for producing the same.

Pruritus is a symptom of most skin disorders, along with pain, which is defined by the unpleasant sensation that causes the urge to scratch in the clinic. The causes of pruritus are mostly skin diseases except kidney failure, biliary obstruction, and parasitic phobias. Skin diseases that are prone to pruritus include atopic dermatitis, urticaria, dry skin, seborrheic dermatitis, psoriasis and insect bites have.

Atopy or atopic syndrome refers to an allergic reaction in which the body becomes extremely sensitive without direct contact with allergic antigens. The cause of the disease is not known in detail, and family history, sex, body mass index, It is known to be a multifactorial disorder caused by multiple factors such as region, pet, birth order, and socioeconomic factors. It is known that symptoms vary depending on the patient's age. As shown in FIGS. 1A and 1B, atopic symptoms are known to increase as the skin moisture saturation increases.

The mechanism of atopic allergy is that when allergens penetrate through the stratum corneum and reach the epidermal cells, macrophages, a kind of white blood cells, digest them and break them down. If the macrophage is unable to process all the large amounts of invaded allergens, information on the presence of allergens that have not yet been processed is sent to T cells (not controlling the immune stimulation and inhibition), a kind of leukocyte, The cells transfer the information back to the eosinophil (eosinophilic leukocyte), a type of leukocyte that collects widely distributed eosinophils into the area where the allergen has penetrated. At this time, eosinophils accumulated in the affected part excessively release the chemical transfer substance (active oxygen), causing inflammation, which is the cause of the allergic reaction.

Atopic dermatitis is an increasing trend worldwide. It is reported that the prevalence rate is 20% of the population. According to the press release of National Health Insurance Corporation in March, 2014, ~ In 2012, the annual average number of medical personnel was 1,040,000, including 490,000 for males and 550,000 for females. In particular, according to the 2012 epidemiological survey of atopic dermatitis shown in Fig. 1C, the number of patients under 9 years of age is close to half of the total number of medical staff, the number of medical staff decreases as the age increases, , 321,000 people, 15 people per 100 people received medical treatment, accounting for 1/3 of the total number of medical personnel

As such, patients with atopic dermatitis and pruritus have been increasing steadily since 1995, and the treatment method is to remove the inhaled antigens such as therapeutic agent, skin moisturizing method by moisturizer, house dust mite, pollen, cockroach, In the case of severe atopic dermatitis and pruritus, ultraviolet light therapy, steroids, immunosuppressants, interferon, immunoglobulin, etc., can be used for the prevention of atopic dermatitis. Lt; / RTI >

However, steroids, immunosuppressants, and antihistamines used as therapeutic agents have side effects such as arrhythmia, hypertension, nephrotoxicity, and drug interactions. Recently, the immunosuppressants such as the recently developed calcineurin inhibitors tacrolimus, FK506 and pimecrolimus, And the incidence of leukemia has been reported to be difficult to treat. Functional, natural cosmetics using herbal extracts or herbal ingredients have emerged as a new alternative to reduce these side effects.

Patent No. 10-0824958 concerning cosmetic compositions containing at least one compound herbal extract, nanoceramides or detergent selected from the group consisting of oranges, mushrooms, mushrooms, 10-0831594. In the registered patent No. 10-0733869, cosmetic raw materials using natural extracts mixed with Gosam, Changsai, white pine bark, casualties, and horseradish have been developed.

Patent patents relating to antimicrobial activity using herbaceous plants include Patent No. 10-2006-0060906, a method for producing an antimicrobial shampoo having antimicrobial activity against Staphylococcus sp., A shampoo composition prepared by the method, , Black bean, black sesame seed, motherwort, iris, camellia, and a mixture of extracts of horse mackerel are known. As a patent relating to skin diseases using Dula, there is a cosmetic composition for improving atopic skin containing a herbal medicine plant extract of Patent No. 10-2010-0044373.

On the other hand, elm has been known as a tree that has been effective for boils and swellings. Recently, there have been reports on antioxidative effect, nitrite removal ability, antibacterial effect against dental caries, and anticancer effect. In Donguibogam, elm trees are plain, flavorless, poisonous, and soft, so that they can easily pass through the feces, eliminate the intestines and upper quadrats, become effective in enteritis, calm the swollen and relieve insomnia. This elm contains various useful substances such as flavonoid, saponin, tannin, and mucus, and is used for treating diseases such as allergic rhinitis, asthma, atopy, inflammation, gastric cancer, edema and mastitis in herbal medicine. Which is not toxic to human body.

In addition, Bower actinidia is a vine-bearing deciduous tree belonging to Quercus variabilis. In the case of leaves and stems of Quercus variabilis, it plays a role of preventing free radicals, which are related with the aging process of the living body, And antioxidants, antioxidants, antioxidants, anti-allergic and anti-inflammatory activities, it can meet the demand and demand for antioxidants, and it is a natural product, so it is relatively safe and has a low side effect. have.

However, no study has been reported on the antimicrobial properties of the extracts of elm or Alaska pollack against pathogenic skin diseases. In addition, there is also no report on the contents of studies on cosmetic compositions using the biodegradable elm or extract of Aloe vera with Lactobacillus

The inventors of the present invention have found that, in the elm or extract, the antibacterial activity against pathogenic skin disease causative microorganisms is almost zero. However, based on the fact that when subjected to the bioconversion process, an excellent antibacterial activity against causative pathogenic skin diseases is given, The present invention has been accomplished by developing a cosmetic composition using an extract and a seedling extract.

Therefore, it is an object of the present invention to provide a method for producing an antimicrobial agent which has excellent antimicrobial activity against pathogenic skin disease causative bacteria obtained by inoculating a specific microorganism into an elm extract and an extract of Alnus species, The present invention provides a cosmetic composition using a natural extract capable of improving atopic pruritus by using a biotransformation elm extract and a bioretransferrin extract, and a method for producing the same.

The objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

In order to achieve the object of the present invention described above, the present invention provides a cosmetic composition for improving atopic pruritus comprising, as an active ingredient, a biodegradable elm extract and a bioconverted elderberry extract having antimicrobial activity against skin disease causative bacteria.

In a preferred embodiment, the antimicrobial activity is imparted via bioconversion of the Lactobacillus sp.

In a preferred embodiment, the skin disease causative microorganism is at least one selected from the group consisting of purulent Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus pyogenes .

In a preferred embodiment, the lactic acid bacteria used for said lactic acid bioconversion are It is Lactobacillus plantaurum .

In a preferred embodiment, the Lactobacillus plantaurm is L. plantarum 299V (DSM 9843).

In a preferred embodiment, the biodegraded elm extract and the bioconverted elderberry extract are present in an amount of 0.1% to 5% by weight of the total composition.

In a preferred embodiment, the biodegraded elm extract and the bioconverted elderberry extract are included in a weight ratio of 3: 7 to 7: 3.

The present invention also relates to a method for preparing a biologically converted elm tree extract and a bioconverted elderberry tree extract; Preparing a precursor cosmetic composition for preparing a precursor cosmetic composition by metering and mixing the prepared bioconverted elm extract and a bioconverted elderberry extract and a cosmetic base; And forming a desired cosmetic formulation with the precursor cosmetic composition. The present invention also provides a method for preparing a cosmetic composition for improving atopic pruritus.

In a preferred embodiment, the step of preparing the biotransformation extract comprises: preparing a liquid medium containing the elm extract and a liquid medium containing an extract of elderberry, respectively, by adding the elm extract and the elderberry extract to the lactic acid bacteria culture composition; An inoculation step of inoculating the respective liquid medium with the lactic acid bacteria; And a culturing step of culturing the lactic acid bacterium so as to carry out the bioconversion of the elm extract and the seedling tree extract in the inoculated state, respectively, to thereby obtain a bioconversion culture.

In a preferred embodiment, the method further comprises a removing step of removing the lactic acid bacteria contained in the bioconversion culture.

In a preferred embodiment, the method further comprises a step of drying the bioconversion culture obtained in the cultivation step, or the step of removing the lactic acid bacteria contained in the bioconversion culture and the step of drying the bioconversion culture in which the obtained lactic acid bacteria have been removed .

In a preferred embodiment, the lactic acid bacteria inoculated in said inoculation step is Lactobacillus plantaurum .

In a preferred embodiment, the Lactobacillus Planta room (Lactobacillus plantaurm) is Lactobacillus plantaurm is L.plantarum 299V (DSM9843) and is inoculated in an amount of 1X10 ³ CFU / ml to 1X10 ⁸ CFU / ml.

In a preferred embodiment, the culturing step is carried out at a temperature range of 25 to 40 degrees for 12 to 96 hours.

First, the cosmetic composition using the natural extract of the present invention can improve atopic pruritus including a biodegradable elm extract and a bioretransferrin extract which have excellent antimicrobial activity against pathogenic skin diseases.

In addition, the method for producing a cosmetic composition of the present invention is characterized in that a specific microorganism is inoculated to an elm extract and an extract of Aloe vera, which have almost no antibacterial activity against pathogenic skin diseases, and cultured under a constant culture condition to obtain an excellent antibacterial activity And a cosmetic composition having the effect of improving atopic pruritus can be prepared using the extract.

FIG. 1A is a photograph showing the symptoms of atopic pruritus, FIG. 1B is a schematic diagram showing the difference between normal skin and atopic skin, and FIG. 1C is a graph showing the current medical treatment status of atopic dermatitis patients in Korea.
FIGS. 2A to 2C are graphs showing atopic sensory test results of a cosmetic composition for improving atopic pruritus (1% biotransformation extract, 4% biotransformation extract) according to an embodiment of the present invention.
3 is a graph showing the hematological test results of the cosmetic composition for improving atopic pruritus (1% biotransformation extract, 4% biotransformation extract) according to an embodiment of the present invention.
4 is a graph showing the IgE concentration in the serum of a cosmetic composition for improving atopic pruritus (1% biotransformation extract, 4% biotransformation extract) according to an embodiment of the present invention.

Although the terms used in the present invention have been selected as general terms that are widely used at present, there are some terms selected arbitrarily by the applicant in a specific case. In this case, the meaning described or used in the detailed description part of the invention The meaning must be grasped.

Hereinafter, the technical structure of the present invention will be described in detail with reference to the accompanying drawings and preferred embodiments.

However, the present invention is not limited to the embodiments described herein but may be embodied in other forms. Like reference numerals used to describe the present invention throughout the specification denote like elements.

The technical feature of the present invention is that the extract of elm and purple tree extract has little antimicrobial activity against pathogenic skin disease bacteria in the state of simple extract, but the biodegradable elm extract and the biotransformed tree extract obtained through the biotransformation process have a pathogenic skin disease The present invention is to provide a cosmetic composition for improving atopic pruritus and a method for producing the same, which comprises a biodegradable elm extract and a bioretransferrinus extract as an active ingredient, which has an excellent antimicrobial activity against causative bacteria.

Here, bioconversion refers to a technique of producing and manufacturing various metabolites using enzymes or microorganisms, and it is possible to produce a useful substance by fermentation under anaerobic conditions or micro-aerobic conditions of microorganisms It is the technology that changes. Bioconversion is being carried out using various materials such as plant, herbal medicines, feed, and silk. It is known that there are various effects of biochemical conversion of herbal medicine using microorganisms. Microbial biotransformation is a characteristic feature of the decrease in the size of the useful substance and the improvement of the function. Production of useful substances such as vitamins, organic acids and bacteriocins produced by the microorganisms also serves as an advantage of biotransformation.

Accordingly, the cosmetic composition for improving atopic pruritus of the present invention contains as an active ingredient a biodegradable elm extract and a bioconverted elderberry extract having antimicrobial activity against a skin disease causative microorganism.

In particular, the bioconversion elm extract and the bioconverted elder tree extract have antimicrobial activity against the causative microorganisms, respectively. Therefore, even if only one of them is effective for the improvement of atopy pruritus, The effect of improving the atopic pruritus was better than that of the control group. As a result, the cosmetic composition for improving atopic pruritus of the present invention is effective for improving atopic pruritus only if it contains both the biologically-transformed elm extract and the biotransformed elderberry extract. For example, the biologically-transformed elm extract and the biotransformed alfalfa extract Can be included to have a weight ratio of 3: 7 to 7: 3.

Here, the causative bacteria of skin diseases may be one or more of purulent Streptococcus pyogenes , Propionibacterium acnes , and Streptococcus pyogenes , which are typical bacteria known to be causative agents of skin diseases. Streptococcus pyogenes is known to be a cause of human laryngitis, tonsillitis, scarlet fever, hunger, and sepsis. Propionibacterium acnes is a pathogenic bacterium present in the skin and intestinal tract. It causes acne, squidoemia, uveitis, conjunctivitis and keratitis. It causes skin rash and chronic inflammation. Staphylococcus aureus is an important cause of skin infection in atopic patients. It is characterized by the secretion of toxins that kill phagocytes such as hemolysin, coagulase, and lucosidine, leading to a pyogenic infectious disease. Propionibacterium acnes exists in the skin or sebaceous glands and is characterized by massive proliferation when the sebaceous glands are active due to increased adolescent hormone activity. When acne is caused by Propionibacterium acnes in adolescent atopic patients, symptoms such as dry skin and increased skin erythema accompanied by itching will appear. In addition, Staphylococcus aureus and Streptococcus pyogenes The symptoms of acne due to tea bacterium infection worsen. According to Yoon et al. (2009), secondary infection of Streptococcus pyogenes was found in atopic patients with fever, purulent exudates, pustules, pustules, etc. and symptoms of sepsis due to bacterial infection were confirmed. In addition, antibiotics, antihistamines, and topical steroids are used to prevent secondary bacterial infections. Streoid phobia causes side effects of steroids. It is known that Staphylococcus aureus is not only the primary cause of atopic dermatitis but also a secondary cause of infections such as skin pore infection, sweat gland blockage, and wound infection. It is known that 90% Infection of S. aureus has been confirmed. In addition, it suggests that using Oriental medicine can inhibit secondary infection of Streptococcus pyogenes and Staphylococcus aureus .

The antimicrobial activity of the biosynthesized elm extract and the biotransformed alfalfa extract contained in the cosmetic composition for improving atopic pruritus of the present invention is imparted through the transformation of the biosynthesis of the elm tree extract and the alfalfa extract, The Lactobacillus can be a Lactobacillus plantaurum . In particular, L. plantarum 299V (DSM 9843), L. plantarum 299V was first isolated in fermented food in 1994, and has been studied in irritable bowel syndrome, mucosal inflammation and the like.

The pH of the biosynthesized elm tree extract and the extract of Alaska pollack tree contained in the cosmetic composition for improving atopic pruritus of the present invention is 4.5 or less, and compared with those of elm and Alaska pollack extracts before the bioconversion, It can be predicted that a component having an excellent antimicrobial activity is generated through bioconversion.

The content of the biologically transformed elm extract and alfalfa extract contained in the cosmetic composition for improving atopic pruritus of the present invention may be in the range of 0.1 wt% to 5 wt% of the total composition weight. If less than 0.1 wt%, the effect of improving atopic pruritus But not more than 5% by weight, but there is no difference in the effect of improving atopic pruritus. Therefore, it is limited to 5% by weight or less in terms of economy.

The cosmetic composition for improving atopic pruritus of the present invention may have various formulations such as solutions, suspensions, lotions, creams, gels, toners, sticks, sprays, aerosols, ointments, cleansing liquid wash, (including those with or without insoluble sheets), make-ups, and the like, as well as other forms of skin, such as, for example, solid bars, pastes, foams, mousses, shaving creams, wipes, strips, patches, electric patches, hydrogels, But may be provided in a variety of product forms including, but not limited to, products, such as foundations, eye liners, and eye shadows. In particular, the form of the cosmetic composition may be according to the particular dermatologically acceptable carrier selected.

Next, a method for preparing a cosmetic composition for improving atopic pruritus according to the present invention comprises: a step of preparing a biotransformation extract to produce a biodegradable elm extract and a biotransformation elder tree extract; Preparing a precursor cosmetic composition for preparing a precursor cosmetic composition by metering and mixing the prepared bioconverted elm extract and a bioconverted elderberry extract and a cosmetic base; And a cosmetic formulation forming step of forming a desired cosmetic formulation with the precursor cosmetic composition.

Herein, the step of preparing a biotransformation extract comprises preparing an aqueous medium containing the elm extract and a liquid medium containing an extract of elderberry, respectively, by adding the elm extract and the elderberry extract to a lactic acid bacterial culture composition; An inoculation step of inoculating the respective liquid medium with the lactic acid bacteria; And culturing the cultured lactic acid bacterium in such a manner that the biologic conversion of the elm extract and the seedlings extract is carried out in the inoculated state, respectively, and, if necessary, removing the lactic acid bacteria contained in the bioconversion culture Removing the lactic acid bacteria contained in the biotransformation culture, and drying the biotransduced culture obtained by removing the lactic acid bacteria, wherein the biotransformation culture obtained in the step of culturing is dried, can do. Inoculation step is Lactobacillus Lactobacillus plantaurm of Planta room (Lactobacillus plantaurm) can be carried out by inoculating the L.plantarum 299V (DSM9843) in an amount of ^{1X10 3 CFU / ml 1X10 8 CFU} / ml. The culturing step may be carried out at a temperature range of 25 to 40 degrees for 12 to 96 hours, but it may be shortened to 24 hours or less. The drying step includes a freeze-drying step, which may be carried out at -80 占 폚 or lower for 36 to 72 hours. In addition, the elm extract and purple tree extract used for the biotransformation can be obtained by extracting at least one of the branches, bark, leaves and fruits of the elm or elder tree with water, C1 to C2 lower alcohols or a mixture thereof as a solvent For example, water, ethanol or a mixture thereof as a solvent.

 The cosmetic base used in the preparation of the precursor cosmetic composition may comprise a dermatologically acceptable carrier (which may also be referred to as "carrier ") and various optional ingredients, including a bioconverted elm extract and a biotransformation tree The extract may be incorporated to transfer the bioconversion extract and other optional ingredients to the skin.

The carrier may comprise one or more dermatologically acceptable solids, semi-solid or liquid fillers, diluents, solvents, extender ingredients, materials, and the like. The carrier may be a solid, semi-solid or liquid. The carrier can be provided in a wide variety of forms. Some non-limiting examples include simple solutions (aqueous or oily), emulsions, and solid forms (e.g., gels, sticks, flowable solids, amorphous materials). The carrier may contain one or more dermatologically acceptable diluents. "Diluent" includes materials in which the bioconverted elm extract and the bioconverted elderberry extract can be dispersed, dissolved, or otherwise incorporated. The hydrophilic diluent may be selected from water, organic hydrophilic diluents such as propylene glycol, polyethylene glycol, polypropylene glycol, glycerol, butylene glycol, 1,2,4-butanetriol, sorbitol esters, 1,2,6- (E. G., C1-C4) and lower molecular weight glycols and alcohols including ethanol, isopropanol, sorbitol esters, butanediol, ether propanol, ethoxylated ethers, propoxylated ethers, Polyols. The carrier may also be in the form of an emulsion, such as an oil-in-water emulsion, a water-in-oil emulsion, and a silicone water-in-oil emulsion. Emulsions can generally be classified as having a continuous aqueous phase (e. G., Water-in-oil and water-in-oil heavy water) or continuous oil phase (e. G., Water and water). The oil phase may include silicone oil, non-silicone oil, such as hydrocarbon oils, esters, ethers, and the like, and mixtures thereof. The aqueous phase may comprise water, for example a solution as described above. However, the aqueous phase may include components other than water, including, but not limited to, water-soluble moisturizers, conditioning agents, antimicrobials, wetting agents and / or other water-soluble skin care actives. The non-water component of the composition comprises a wetting agent such as glycerin and / or other polyols. The emulsion may also contain from about 1% to about 10% or from about 2% to about 5% of an emulsifier based on the weight of the carrier. The emulsifier may be non-ionic, anionic or cationic.

A wide variety of optional ingredients may be included in the cosmetic base. For example, the cosmetic base may be selected from the group consisting of absorbents, abrasives, anticaking agents, defoamers, antimicrobials, binders, biological additives, buffers, bulking agents, chemical additives, cosmetic biocides, denaturants, Emollients, skin soothing agents, antioxidants, antioxidants, antioxidants, antioxidants, antioxidants, antioxidants, antioxidants, antioxidants, antioxidants, Sunscreen, sunblock, ultraviolet light, sunscreen, sunscreen, sunscreen, sunscreen, sunscreen, sunscreen, sunscreen, sunscreen, sunscreen, sunscreen, sunscreen, (S), an anti-inflammatory agent, an anti-inflammatory agent, an anti-inflammatory agent, an anti-inflammatory agent, an anti-inflammatory agent, and various functional ingredients effective for the skin care such as depigmenting agents, desquamation agents / exfoliants, organic hydroxy acids, vitamins and derivatives thereof, natural extracts, and collagen. Such other materials are known in the art.

Example 1

1. Biological conversion extract preparation step

(1) Medium preparation step

① Preparation of elm and extracts

100 g of dried elm and 1 L of water are placed in a hot water bath and hydrothermal extraction is carried out. Extracts of elm were extracted from 500 ml of hot water extract obtained by hot water extraction using a paper filter to remove impurities.

In addition, 100 g of dried seedlings and 1 L of water are placed in a hot water boiler and subjected to hot water extraction. Extracts were extracted with 500 ml of hot water extracts and extracted with impurities using a paper filter.

② MRS broth powder, which is a lactic acid bacteria culture, was dissolved in the elm extract and Mulberry tree extract obtained by adding 55 grams of MRS broth powder per 1 liter of elm extract and elderberry extract, MRS broth powder containing elm extract and MRS broth containing ellah tree extract were prepared by dissolving 55 grams of MRS broth powder per 1 liter of wood extract and sterilizing.

(2) Inoculation step

Lactobacillus Lactobacillus planta 299V (DSM9843) was inoculated to the MRS medium containing the elm extract and MRS medium containing Mulberry tree extract at a concentration of 1 × 10 ⁶ CFU / ml.

(3) Culture step

Lactobacillus inoculated with the extracts of elm and bark extracts were incubated at 37 ° C for 24 hours to obtain bioconversion cultures of the elm extract and the elderberry extract.

(4) Removal step

Each biotransformed culture was subjected to centrifugation at 7000 rpm for 30 minutes and then filtered using a 0.45 ㎛ syringe filter to remove the lactic acid bacteria contained in the biotransformed culture to obtain a biotransformation elm extract and a biotransformant extract Liquid phase.

(5) Drying step

The biotransformed elm extract and the bioconverted elderberry extract were further subjected to a drying step comprising lyophilization for 72 hours at -80 ° C for 36 hours respectively, Powder form.

2. Preparation step of precursor cosmetic composition

A precursor cosmetic composition was prepared by weighing and mixing raw materials of 1 to 14 in a blending ratio as shown in Table 1 below. Here, the biotransformation extract is a mixture of powdery biotransformed elm extract and biotransformed persimmon extract at a weight ratio of 1: 1.

3. Cosmetic Formulation Step

The precursor cosmetic composition is dissolved in water at 95 DEG C, and the dissolved precursor cosmetic composition is mixed using an AGI mixer. The mixed precursor cosmetic composition was emulsified using a HOMO mixer. The emulsified precursor cosmetic composition was cooled to 25 캜 to prepare an emulsion type cosmetic composition 1 for improving atopic pruritus (1% biotransformation extract).

Example 2

The cosmetic composition 2 for improving atopic pruritus (4% biotransformation extract) was prepared in the same manner as in Example 1, except that 4% by weight of the biotransformation extract was included.

Experimental Example 1

The pH values of the biotransformed elm extract and the bioconverted elderberry extract after bioconversion using the elm extract obtained from Example 1, the extract of Mulberry tree and the lactic acid bacterium were measured and the results are shown in Tables 2 and 3, respectively.

From Table 2, the pH of the elm extract before the bioconversion was 6.15, and the pH of the control MRS broth and biosynthesized elm extract was 4 and 4.19, respectively.

From Table 3, it can be predicted that the pH of the seedling extract before the bioconversion was 6.2, and the pH of the control MRS broth and the biotransformed seedling extract were 3.9 and 3.98, respectively, .

Experimental Example 2

The antimicrobial effect of the cultured lactic acid bacterium, the elm extract obtained in Example 1 and the biologically transformed elm extract (liquid phase) against the skin disease causative bacteria was confirmed, and the results are shown in Table 4.

Here, Lactobacillus plantarum 299V was cultured in MRS medium and elm extract MRS medium. The pathogens used were confirmed to have antimicrobial inhibitory effects on three species of Propionibacterium acnes KCTC 3314, Streptococcus pyogenes KCCM 11873 and Staphylococcus aureus KACC 13236 . Streptococcus pyogenes KCCM 11873 was cultivated in a medium containing 5% Sheep blood in a TS (Tryptic-Soy) medium, in a medium of Staphylococcus aureus KACC 13236 was cultured in a TS (Tryptic-Soy) medium.

Specifically, the paper disc method was used to investigate the antibacterial activity. Lactobacillus cultures, elm extracts, and biotransformation elm extracts were spotted on 8 mm paper discs in 100 μl aliquots at 1 × 10 ⁶ CFU / ml, respectively. The medium on which the paper discs were germinated was cultured for 24 hours at 37 ° C to confirm whether or not growth inhibition rings were produced. The results are shown in Table 4

As can be seen from the following Table 4, the antimicrobial activity was not detected in all the skin diseases caused by the elm extract, and the antimicrobial activity was confirmed in S. aureus in the Lactobacillus MRS culture medium. However, antimicrobial activity was also confirmed in P. acnes and S. pyogenes , which were not detected in the MRS culture broth or elm extract, in the biotransformed elm extract, and the antimicrobial activity of S. aureus was increased by 7%.

Experimental Example 3

The antimicrobial effect against the causative bacteria of skin diseases was confirmed in the same manner as in Experimental Example 2 except that the culture broth of Lactobacillus acidus, Mulberry Tree Extract, Bioconverted Mulberry Tree Extract (Liquid) and Bioconverted Mulberry Tree Extract (Powder) Are shown in Table 5 below.

As can be seen from Table 5, antimicrobial activity was not detected in all the skin disease causative organisms in the extract of Mulberry tree, and the antimicrobial activity was confirmed in S. aureus in the MRS culture of lactic acid bacteria. The antimicrobial activity of all the skin disease causative organisms was confirmed in the biotransformation tree extract, and the antimicrobial activity of S. aureus was increased by 13%. In the case of the biotransformation tree extract, the antimicrobial activity was increased from at least 6% to at most 18% before the concentration.

Experimental Example 4

The sensory evaluation was carried out by using NC / Nga mouse, which is widely used in atopy-related efficacy evaluation tests, to compare the cosmetic composition 1 for improving atopic pruritus (1% biotransformation extract) obtained in Examples 1 and 2 and the cosmetic composition for improving atopic pruritus 2 (4% biotransformation extract) and the results are shown in Figures 2a to 2c. The sensory evaluation for atopic dermatitis was generally performed by transdermal administration, and the administration frequency and administration period were 7 days / week, 2 times / day for 14 days daily. This evaluation method is a clinical visual evaluation method commonly used in atopic dermatitis. The degree of severity of atopic dermatitis is expressed as the sum of scores evaluated for each of the following five items. Evaluation items include erythema, pruritus & dry skin, edema & excoriation, erosion, and lichenificatnion. Each item was graded as having no symptoms (0 point), symptom weakness (1 point), normal (2 points), and severe (3 points) ), And a score of at most 15 (severely symptomatic of all items).

As shown in FIGS. 2A to 2C, the atopic symptoms were continuously observed during the administration period of the atopic induction group, while the cosmetic compositions for improving atopic pruritus of the present invention, i.e., the 1% biotransformation extract and the 4% , Symptoms such as erythema, pruritus & dryskin, edema & excoriation, erosion, and lichenification were observed to improve.

Experimental Example 5

Hematologic tests were performed on all of the experimental mice that caused atopic dermatitis. The animals were fasted for more than 18 hours before being drawn and were anesthetized with isoflurane and then collected from the abdominal artery. Approximately 2 ml of blood was collected in a CBC bottle containing EDTA (EDTA 2K, BD vacutainer) and analyzed for leukocyte, neutrophil, lymphocyte, mononuclear cell, eosinophil, basophil, and staining cells using a blood analyzer And the results are shown in Fig.

As shown in FIG. 3, in the hematological test results, the levels of atropia-related neutropils and eosinophils were decreased in all the test substance-administered groups as compared with the atopy-induced group. There was also a statistically significant increase in the percentage of lymphocyte compared to the atopy-induced group, which is considered to be an inflammatory symptom due to atopic dermatitis.

Experimental Example 6

In the case of mice with atopic dermatitis, the IgE concentration in the control mice and the mice in the control mice was estimated to be rapidly increased according to the age of the mice compared with the control mice. At day 14 after atopy induction, the blood of the mice was collected from the abdominal artery. The serum of the collected blood was separated to measure IgE, and the absorbance change was measured using an ELISA (enzyme linked immunosorbent assay) kit. The results are shown in FIG.

As shown in FIG. 4, the IgG level in the serum decreased in all the test substance-administered group (P < 0.01) compared to the atopy-induced group.

Experimental Example 7

Atopic allergic rhinitis relieving sensory test was carried out on 10 atopic patients with similar degree of atopic pruritus, and they were screened twice for 1 week in the morning (8 o'clock) and evening (20 o'clock) Elm extract, biotransformed seedlings extract and 1% biotransformed extract were applied to perform atopic eczema relief sensory test. The results are averaged and shown in Table 6 below.

From Table 6, it can be seen that only one of the biosynthesized elm extract and the biotransformed elderberry extract has some degree of atopy mitigation effect. However, It can be seen that the cosmetic composition for improving atopy of the present invention (1% of biotransformation extract) is more effective in alleviating atopic pruritus, and it can be confirmed that the biotransformed elm extract and the biotransformation extract have a synergistic effect.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be taken by way of limitation, Various changes and modifications will be possible.

Claims (14)

A cosmetic composition for improving atopic pruritus, comprising as an active ingredient, a biodegradable elm extract and a bioconverted elderberry extract having antimicrobial activity against causative bacteria of skin diseases.
The method according to claim 1,
Wherein the antimicrobial activity is imparted through the biosynthesis of the lactic acid bacterium of the elm extract and the extract of aquatic plant extract to improve the atopic pruritus.
The method according to claim 1,
The skin disorder causing bacteria is pyogenic streptococci (Streptococcus pyogenes), acne causing bacteria (Propionibacterium acnes) and atopic pruritus cosmetic composition for improving, characterized in that at least one of Streptococcus pyogenes.
3. The method of claim 2,
The lactic acid bacteria used for the conversion of the above- A cosmetic composition for improving atopic pruritus characterized by being Lactobacillus plantaurm .
5. The method of claim 4,
Wherein the Lactobacillus plantaurm is L. plantarum 299V (DSM 9843).
The method according to claim 1,
Wherein the biodegraded elm extract and the biodegraded elderberry extract are present in an amount of 0.1 wt% to 5 wt% of the total weight of the composition.
The method according to claim 6,
Wherein the biodegradation elm extract and the bioconverted elderberry extract are contained in a weight ratio of 3: 7 to 7: 3.
A biotransformation extract preparation step for producing a biodegradable elm extract and a bioconverted elder tree extract;
Preparing a precursor cosmetic composition for preparing a precursor cosmetic composition by metering and mixing the prepared bioconverted elm extract and a bioconverted elderberry extract and a cosmetic base; And forming a desired cosmetic formulation with the precursor cosmetic composition. &Lt; RTI ID = 0.0 &gt; 11. &lt; / RTI &gt;
9. The method of claim 8,
The preparation step of preparing the biotransformation extract comprises preparing a liquid medium containing the elm extract and a liquid culture medium containing the ellah tree extract by adding the elm extract and the elderberry extract to the lactic acid bacteria culture composition respectively; An inoculation step of inoculating the respective liquid medium with the lactic acid bacteria; And culturing the transformed cultured Escherichia coli to obtain a bioconverted culture by culturing the lactic acid bacterium in the inoculated state so that the biologic conversion of the elm extract and the seedlings extract is carried out, respectively.
10. The method of claim 9,
Further comprising a removing step of removing the lactic acid bacteria contained in the biotransformation culture.
10. The method of claim 9,
The method further comprises a step of drying the bioconversion culture obtained in the culturing step or further comprising the step of removing the lactic acid bacteria contained in the bioconversion culture and drying the bioconversion culture in which the obtained lactic acid bacteria have been removed Wherein the atopic pruritus is at least one of the following.
10. The method of claim 9,
Wherein the lactic acid bacteria inoculated in the inoculation step is Lactobacillus plantaurum .
13. The method of claim 12,
Wherein said Lactobacillus plantaurm is L. plantarum 299V (DSM9843), and wherein said Lactobacillus plantaurm is inoculated in an amount of 1 X 10 ³ CFU / ml to 1 X 10 ⁸ CFU / ml. Way.
10. The method of claim 9,
Wherein the culturing step is performed for 12 hours to 96 hours at a temperature ranging from 25 degrees C to 40 degrees Celsius.

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