KR20180002079A - Composition containing ginseng berry extract using processing of herbal medicine - Google Patents

Abstract

The present invention relates to a composition containing a processed ginseng berry extract by applying poje processing method which is an oriental medicine processing technology. More specifically, the present invention relates to a composition which brings skin-whitening, anti-aging, and moisturizing efficacy by containing a ginseng berry extract processed by Poje using ginseng berries which have unique components and compositions among aerial parts of ginseng.

Description

[0001] The present invention relates to a composition containing ginseng fruit extract,

The present invention relates to a composition containing a processed ginseng fruit extract using a forage processing technology, and more particularly, to a composition containing a ginseng fruit extract having a unique composition and composition among the above-mentioned ginseng, Thereby providing an effect of whitening, anti-aging, moisturizing and the like.

Foje (匍 制) is a pharmaceutical technology (pharmaceutical technology) that changes the original properties of medicines by processing the medicines based on the oriental theory. Some medicines are toxic or very strong in nature and can not be taken directly. Some medicines can not easily be stored for a long time and others can be used only after removing some parts and parts. Also, even if the same medicines are used, there is a case where the properties of the raw material and the raw material are not same or different from each other. Before using this medicinal product, it must be processed, which is called a medicinal plant.

Foams are sometimes processed together by adding a supplement, and the use of the supplement is to achieve the purpose of the drug according to the dialect. Because of the variety of types of pills used in drug formulations, they have different properties and actions, and the resulting action of the medicinal formulations is different. There are a myriad of commonly used types of supplements, but generally they can be broadly categorized as liquid supplements and solid supplements. Liquid ingredients include alcohol, vinegar, honey, ginger juice, licorice juice, black bean juice, saline, unmeaned water, milk, urine (urine of children under the age of 7) and lime water. Solid foods include rice, bran, Tofu, earth, ply, sand, etc. are used.

The purpose of purifying herbal medicine is to ① clean the drug and make it easy to store ② reduce or eliminate the toxicity and side effects of the drug ③ change the drug to make the drug more effective ④ improve the therapeutic effect of the drug And (5) to remove the bad smell and taste of the medicinal materials so as to make them good for taking.

On the other hand, the skin is the largest organ completely covering the surface of the body, and forms the outermost protective film for protecting the body from the outside. As the age increases, aging progresses like other body organs. Skin is often a criterion for aesthetic judgment, and attention is now focused on prevention and inhibition of skin aging.

Looking at the structure of the dermis, the collagen fibers (collagen) form a three-dimensional structure, elastic fibers between them (elastin) acts like a spring to maintain the elasticity of the skin. As age grows, collagen fibers in the dermis are destroyed and elastic fibers are formed in the body by malondialdehyde (MDA) to form crosslinks between molecular chains, resulting in poor elasticity. As the aging progresses, the amount of the substances that fill the extracellular space as well as the fiber that maintains the skin structure is reduced. The healthy stratum corneum contains 15 ~ 20% of water, and when the moisture falls below 10%, the skin becomes dry, the gloss and elasticity disappear, and the wrinkles increase. All of these results cause the aged skin to lose elasticity and wrinkle.

In addition, the other part where attention is focused on the skin is related to the skin color, and the distribution and the amount of melanin in the skin occupy the largest portion among the determinants of the skin color. Melanin is produced in melanocytes. These melanocytes contain enzymes such as tyrosinase. They work together to form an enzyme called tyrosine, which is always present in the body. Thereby forming a dark brown pigment melanin. The melanin thus formed migrates to the epidermal cell called keratinocyte through the dendrites of melanocytes. Here, melanin forms a hat-like structure around the nucleus, which protects genes from ultraviolet rays and eliminates free radicals, thereby protecting the intracellular proteins. In vivo, there is no enzyme that breaks down melanin, but it is removed as the keratinocyte falls away from the skin when it falls off the epidermis. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results. There are various factors that affect the production of melanin. The increase of melanin production caused by ultraviolet rays and the pigmentation thereof are very important in the cosmetics field. The basic mechanisms of pharmaceuticals incorporated into whitening cosmetics for the prevention of pigmentation are inhibition of tyrosinase action, inhibition of tyrosinase production, inhibition of melanin-producing mediators, reduction of existing melanin and inhibition of photo-oxidation, promotion of melanin excretion, and And ultraviolet rays cut.

According to the desire of women who want to have white skin even in the ultraviolet exposure environment, the demand for prevention and improvement of skin pigmentation abnormality and hyperpigmentation is increasing more and it is necessary to develop a whitening product which prevents excessive melanin production, Many efforts have been made during this period. Specific examples thereof include inhibitors which inhibit tyrosinase activity such as kojic acid and arbutin, hydroquinone, vitamin A, vitamin C and derivatives thereof. However, they are limited in their use due to safety to the skin, stability in the formulation and insufficient whitening effect.

In recent years, much research has been conducted on cosmetic compositions using natural extracts, which are reported to have remarkable use of natural products in recent years, excellent moisturizing ability, aging-improving function, antioxidant effect, and particularly excellent in skin aging-improving effect. However, the compositions using natural extracts extracted by conventional methods are not effective in whitening, wrinkling, and antioxidation as desired, and are difficult to maintain and control the activity of these extracts.

Accordingly, the present inventors have searched for a substance capable of improving the skin condition and found that the composition containing the processed ginseng fruit extract by utilizing the herbicide processing technology as a herbal processing technology is effective for improving the skin condition such as anti-aging, moisturizing, And completed the present invention.

Accordingly, an object of the present invention is to provide a composition containing natural products safe for skin, which is excellent in the skin condition improving effect such as anti-aging, moisturizing, whitening, etc., by preparing a ginseng fruit extract using fungicide and containing the extract as an active ingredient .

In order to attain the above object, the present invention provides a composition containing a ginseng fruit extract using a fungus.

The composition of the present invention is excellent in the effect of inhibiting the expression of elastase and collagenase by containing the extract of fruit extract using Fojer, so that it has excellent anti-aging and elasticity improving effect. It suppresses skin aging and increases skin moisture retention And has an excellent effect on skin whitening, thereby providing an effect of improving the overall skin condition while being safe for the skin.

The composition of the present invention contains a ginseng fruit extract using a feces, and can be provided as a cosmetic composition or a food composition.

In addition, the composition of the present invention provides effects such as anti-aging, moisturizing, whitening and the like.

Hereinafter, the present invention will be described in more detail.

Ginseng (Panax ginseng C.A. Meyer) is a herb that belongs to the genus Ogawa and Ginseng. It has been used in Korea, China and Japan for over 2,000 years and has been used empirically to prevent disease and prolong the life span. The efficacy and effect of ginseng which has been known so far can be determined by the action of the central nervous system, the anticarcinogenic action, the anticancer activity, the immune function regulating action, the anti-diabetic action, the hyperfunction of liver function, the improvement of cardiovascular disorder, (Korean Ginseng & Tobacco Research Institute, 56-112, 1996). In addition, it has been reported that the antioxidant activity and antioxidant activity of osteoporosis are improved.

Ginsenoside, which is a representative physiologically active ingredient of ginseng, is distributed evenly on the ground and underground of ginseng. Especially, ginsenoside (root), ginseng leaf and ginseng berries have not only ginsenoside content but also composition (Attele AS et al, Biochem Pharmacol, 58: 1685-1693, 1999).

Meanwhile, the method for extracting ginseng fruit used in the present invention is as follows.

(1) Foam processing step.

The ginseng fruit may be boiled, steamed, roasted, roasted or boiled, or the roots may be mixed with the roasted ginseng. In case of boiling, 0.1-1 hour at 100-180 ℃, 1 - 48 hours at 150-200 ℃ for steaming, 0.1-1 hour at 100-300 ℃ for roasting, 0.5 - 5 hours at 180-300 ℃ for roasting . ≪ / RTI >

(2) obtaining an extract.

The feces extract of the ginseng fruit obtained by the above step (1) is obtained by using water or an organic solvent. The organic solvent may be at least one selected from the group consisting of ethanol, methanol, butanol, ether, ethyl acetate and chloroform, or a mixed solvent of these organic solvents and water may be used. Preferably, 80% ethanol is used.

The cosmetic composition of the present invention contains, as an active ingredient, ginseng fruit extract obtained by the above method in an amount of 0.001-30.0% by weight based on the total weight of the composition.

The composition according to the present invention can be manufactured as a cosmetic composition or a food composition, and the composition according to the present invention can be produced as a transdermal composition or an oral composition.

Specifically, the cosmetic composition of the present invention is not particularly limited in its formulation. For example, the cosmetic composition of the present invention can be used as a cosmetic composition for a cosmetic composition, such as a softening agent, a nutritional lotion, a milky lotion, a massage cream, And may be a cosmetic composition having a formulation of a spray cosmetic. For example, softening longevity, convergent lotion, nutritional lotion, nutritional cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, pack, powder, body lotion, body cream, body oil, body essence , And may also be a cosmetic composition having transdermal delivery formulations such as lotions, ointments, gels, creams, patches or sprays

Examples of the formulations for oral administration of the present invention include tablets, pills, hard and soft capsules, liquids, suspensions, emulsions, syrups, granules, etc. These formulations may contain, in addition to the active ingredient, , Dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (such as silica, talc, stearic acid and its magnesium or calcium salts, and polyethylene glycols). Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally mixed with starch, agar, alginic acid or its sodium salt Such as disintegrants, absorbents, coloring agents, flavoring agents, and sweetening agents. Tablets can be prepared by conventional mixing, granulating or coating methods. The external preparation for skin includes an external preparation for skin and an injection, and the external preparation for skin is preferably an ointment, and may be an isotonic aqueous solution or suspension in the form of injection.

In addition, when the present invention is formulated into a food composition, dairy products such as meat, sausage, bread, chocolates, candies, snacks, confectioneries, noodles, gums and ice cream, various soups, drinks, tea, drinks, And the like, and includes, but is not limited to, food in a conventional sense.

In addition, in the respective formulations, components other than the above-mentioned essential ingredients can be appropriately selected and blended by those skilled in the art according to the kind of the other formulations or the purpose of use.

The composition according to the present invention has an excellent anti-aging effect, thereby providing an effect of preventing and preventing wrinkles and enhancing skin moisturizing power. Also provided is a skin whitening effect, the composition according to the present invention can improve overall skin condition.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are intended to illustrate the present invention, but the scope of the present invention is not limited thereto.

[Comparative Example 1] Production of ginseng fruit product

To 1 kg of dried ginseng fruit, 5 L of 80% ethanol aqueous solution was added, and the mixture was refluxed three times and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a ginseng fruit extract.

[Example 1] Preparation of ginseng fruit jujube

1kg of clean ginseng fruit was evenly sprinkled with a certain amount of yellow liquor (10 ~ 30%) and soaked so as to become damp enough to absorb all of the liquor, and this was put in a suppository container and fried at 100 to 180 ° C for 15 to 30 minutes. The ginseng fruits were taken out and cooled, and then 5 liters of 80% ethanol aqueous solution was added, refluxed three times, and then immersed at 15 ° C for 1 day. Thereafter, the residue and filtrate were separated by filtration and centrifugation through a filter cloth, and the separated filtrate was concentrated under reduced pressure to obtain a ginseng fruit jujuba extract.

[Example 2] Manufacture of ginseng fruit gruel

1kg of dried ginseng fruit, 150 ~ 300g of vinegar was sufficiently absorbed into the medicinal material and roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and then dried in the shade. To this was added 5 liters of an 80% aqueous ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a ginseng fruit root extract.

[Example 3] Production of ginseng fruit chrysotile

1 kg of dried ginseng fruit is carbonized outside the ginseng at 230 ~ 300 ℃. When it becomes black, heating is stopped. After cooling at room temperature, 5 liters of 80% ethanol aqueous solution is added and the mixture is refluxed for 3 times. . Thereafter, the residue and filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain ginseng fruit chrysanthemum extract.

[Example 4] Production of ginseng fruit millet

200-300g of honey was absorbed into 1kg of dried ginseng fruit sufficiently and then roasted at 100 ~ 160 ℃ for 10 minutes ~ 1 hour and then dried in the shade. To this was added 5 liters of an 80% aqueous ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and the filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a ginseng fruit mill extract.

[Example 5] Preparation of ginseng fruit salt

1kg of dried ginseng fruit is mixed well with salt water (2 ~ 3%), sealed and completely absorbed. The ginseng fruit which has been completely absorbed with salt water is put into a supergiant container at 100 ~ 180 ℃ and roasted for 10 minutes ~ 1 hour Then I dried it in the shade. To this was added 5 liters of an 80% aqueous ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a ginseng fruit seed extract.

[Example 6] Manufacture of ginseng fruit juice

Fresh ginger was crushed, and then double water was added to the mixture. The mixture was squeezed and squeezed. The ginger juice was prepared by repeating this 2-3 times. The ginger juice was sprayed with 100-150 g of ginger juice in 1 kg of the ginger juice, and then the ginger juice was poured into the container at 100-180 ° C., roasted for 10 minutes to 1 hour, and then dried in the shade . To this was added 5 liters of an 80% aqueous ethanol solution, refluxed three times, and then immersed at 15 DEG C for 1 day. Thereafter, the residue and filtrate were separated by filtration through a filter cloth and centrifugation, and the separated filtrate was concentrated under reduced pressure to obtain a ginseng fruit extract.

[Test Example 1] Measurement of inhibitory effect on elastase expression

The inhibitory effect of the ginseng fruit and ginseng fruit extract obtained from Comparative Example 1 and Examples 1 to 6 on elastase production was measured in comparison with tocopherol and EGCG.

Human fibroblasts were plated at 5,000 cells / well in a 96-well microtiter plate containing DMEM containing 2.5% fetal bovine serum (Dulbecco's Modified Eagle's Media) Lt; / RTI > until growth. Thereafter, the cells were cultured in serum-free medium for 24 hours, treated with ginseng fruit and ginseng fruit extract, tocopherol and EGCG of Comparative Example 1 and Examples 1 to 6 dissolved in serum-free medium for 24 hours at a concentration of 10 ^-4 mol Then, the cell culture fluid was collected.

The degree of production of elastase in the cell culture was measured using a commercially available elastase measuring device (Amersham Pharmacia, USA). First, the cell culture medium collected on a 96-well plate uniformly coated with primary ELASA antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours. After 3 hours, the secondary elastin antibody bound to the chromophore was placed in a 96-well plate and reacted again for 15 minutes. After 15 minutes, the color development inducing substance was added to induce color development at room temperature for 15 minutes, and 1M sulfuric acid was added again to stop the color development reaction. The color of the reaction solution became yellow, and the degree of yellow was different according to the progress of the reaction.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer and the degree of expression of elastase was calculated by the following equation (1) Respectively. At this time, the reaction absorbance of the cell culture fluid collected from the non-treated group was used as a control.

compound Expression level (%) Untreated group 100 Tocopherol 89 EGCG 67 Comparative Example 1 90 Example 1 71 Example 2 72 Example 3 77 Example 4 82 Example 5 86 Example 6 79

As shown in Table 1, the degree of expression of elastase in Examples 1 to 6 in vitro was lower than that of Comparative Example 1 of the ginseng fruit extract, Suggesting that the effect of inhibiting the expression of the enzyme is better. In addition, compared with tocopherol and EGCG, which are known as inhibitors of elastase expression, the inhibitory effect on elastase expression of the extract of ginseng root according to the present invention is superior to tocopherol and is similar to that of EGCG.

[Test Example 2] Collagenase (MMP-1)

The inhibitory effect of the extracts of ginseng and ginseng extract prepared from Comparative Example 1 and Examples 1 to 6 on collagenase production was compared with that of retinoic acid.

Human fibroblasts were plated at 5,000 cells / well in a 96-well microtiter plate containing 2.5% fetal calf serum-containing DMEM (Dulbecco's Modified Eagle's Media) And incubated at 37 ° C in an incubator with CO _{2 of} 5% until it grew to about 80%. Then, the ginseng fruit and ginseng fruit extract of Comparative Example 1 and Examples 1 to 6 were treated for 24 hours at a concentration of 10 ^-4 mol, and the cell culture solution was collected.

The degree of collagenase production in the cell culture was measured using a commercially available collagenase assay device (Amersham Pharmacia, USA, Catalog #: RPN 2610). First, the cell culture solution collected on a 96-well plate uniformly coated with primary collagenase antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours. After 3 hours, the second collagen antibody bound to the chromophore was placed in a 96-well plate and reacted for another 15 minutes. After 15 minutes, the color development substance (3,3 ', 5,5'-tetramethylbenzidine, sigma) was added and color development was induced at room temperature for 15 minutes. When 1 M sulfuric acid was added to stop the color development reaction, the color of the reaction solution became yellow And the degree of yellowing was different according to the progress of the reaction.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer and the degree of synthesis of collagenase was calculated by the following equation (2) Respectively. At this time, the reaction absorbance of the cell culture fluid collected from the non-treated group was used as a control.

compound Expression level (%) Untreated group 100 Retinoic acid 75 Comparative Example 1 93 Example 1 78 Example 2 80 Example 3 77 Example 4 82 Example 5 84 Example 6 79

As shown in Table 2, the degree of collagenase expression of Examples 1 to 6 in vitro was lower than that of Comparative Example 1 of the ginseng fruit extract, and the collagenase of the ginseng fruit extract of the present invention Suggesting that the effect of inhibiting the expression of the enzyme is better. In addition, in comparison with retinoic acid, which is known as a collagenase expression inhibitor, the inhibitory effect of collagenase on the expression of collagenase in the extract of ginseng fruit according to the present invention is similar to that of retinoic acid.

From these results, it can be confirmed that the extract of ginseng root according to the present invention has an effect of inhibiting the substrate metalloprotease (MMP-1).

[Test Example 3] Confirmation of improving skin elasticity

To confirm the skin elasticity improving effect, the lotion of Comparative Example 1 and Formulations 1 to 6 was prepared using the ginseng fruit and ginseng fruit extract obtained in Comparative Example 1 and Examples 1 to 6, with the composition shown in Table 3 below Ginseng fruit or ginseng fruit lotion containing no extracts was prepared as a control.

Component (content:% by weight) Control group Comparative Formulation Example 1 Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Formulation Example 5 Formulation Example 6 Cetearyl glucoside 1.5 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Cetyl octanoate 3.0 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Methylparaben 0.14 0.14 0.14 0.14 0.14 0.14 0.14 0.14 Disodium ethylenediaminetetraacetate 0.02 0.02 0.02 0.02 0.02 0.02 0.02 0.02 Comparative Example 1 - 10 - - - - - - Example 1 - - 10 - - - - - Example 2 - - - 10 - - - - Example 3 - - - - 10 - - - Example 4 - - - - - 10 - - Example 5 - - - - - - 10 - Example 6 - - - - - - - 10 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100

In order to compare skin elasticity improvement effects of the control group, Comparative Formulation Example 1 and Formulation Examples 1 to 6, 160 individuals of 30 to 40 years old women were divided into eight groups of 20 persons as one group. The experimental method is as follows.

To each group, control lotions, Comparative Formulation Example 1, or Formulation Examples 1 to 6 were applied to each eye, and a predetermined amount of the lotion was applied once a day for 12 weeks. At this time, the lotion of the control group was applied to the left side of the eye of the subject, and the lotion of Comparative Formulation Example 1 or Formulations 1 to 6 was applied to the right side. After 12 weeks, skin elasticity of each group was measured and averaged using a cutometer (SEM474, Courage + Khazaka electronic GmbH, Germany) capable of measuring skin elasticity, and the results are shown in Table 4 .

compound Skin elasticity improvement (%) Control group 18 Comparative Formulation Example 1 39 Formulation Example 1 58 Formulation Example 2 46 Formulation Example 3 48 Formulation Example 4 47 Formulation Example 5 50 Formulation Example 6 55

The results of Table 4 show that when the lotion formulations of Formulation Examples 1 to 6 containing the extract of ginseng root extract according to the present invention were used, the elasticity of the skin was improved to about three times as compared with the control group not containing the ginseng fruit extract And the improvement in skin elasticity is remarkably superior to that in the case of using the cosmetic lotion of Comparative Formulation Example 1 containing a simple extract of ginseng fruit.

Accordingly, it can be confirmed that the cosmetic composition containing the ginseng fruit extract of the present invention is very effective for improving skin elasticity.

[Test Example 4] Measurement of inhibitory effect on melanin formation using mouse pigment cells

The cells were cultured in DMEM supplemented with 10% fetal bovine serum, 100 nM 12-O-Tetradecanoyl (10 nM) Acetate, and 1 nM cholera toxin at 37 ° C in the presence of 5% CO ₂ . The cultured Mel-Ab cells were removed with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 10 ⁵ cells / well (cells / well) in a 24-well plate. And cultured. Hydroquinone and the ginseng fruit extract of Examples 1 to 6 were used as test substances at a concentration of 10 ppm. At this time, the hydroquinone was used as a positive control. Then, the culture solution was removed, washed with PBS, and the cells were dissolved with 1 N sodium hydroxide, and the absorbance at 400 nm was measured. The rate of inhibition of melanin production was calculated according to the following equation (3) Method).

Test substance Melanin production inhibition rate (%) Untreated group 100 Example 1 37.4 Example 2 35.2 Example 3 36.8 Example 4 38.3 Example 5 34.9 Example 6 37.1 Hydroquinone (positive control) 41.1

As shown in Table 5, it can be confirmed that the extract of ginseng root extract of the present invention shows a degree of inhibition of melanin formation similar to that of hydroquinone, which is a known whitening substance.

[Test Example 5] Confirmation of activation of PPARs

HaCaT, a human keratinocyte, was subcultured in a DMEM medium containing 10% fetal bovine serum and phenol red-free medium was used to eliminate the effect of estrogen on phenol red . The plasmid is a promoter of the PPARs response element (PPRE), which is activated by binding of a plasmid having PPAR?, PPAR? /?, PPAR? Gene and ligand-binding PPARs after a universal promoter expressed under ordinary culture conditions. A plasmid in which a firefly luciferase gene is used as a reporter and a universal promoter to be used as a reference is coupled with a β-galactosidase gene (Proc. Natl. Acad. Sci. USA, 91 (1994) 7355-7359).

HaCaT cells were plated in a 24 well plate at a concentration of 5x10 < ⁴ > and cultured for 24 hours. Then, the plasmid gene was transiently transfected. After incubation for 24 hours, the cells were washed with phosphate buffered saline (PBS), and 7,3 ', 4'-trihydroxyisoflavone and a positive control were added to PPARs ligand (Wy-14,643, PPARγ ligand, troglitazone , &Quot; TGZ ")) were treated at the specified concentrations. As the negative control group, the ethanol treated group used for lysing the samples was used. After culturing for 24 hours, the cells were washed with PBS, and the luciferase activity was measured by harvesting the cells. The results are shown in Table 6 and Table 7.

Table 6 shows the result of transfection of PPAR promoter reporter plasmid and PPARa expression plasmid into the HaCaT cell line used in the study, followed by treatment with the drug and measurement of luciferase activity. Table 7 shows the result of the simultaneous transfection of the PPAR gamma expression plasmid and the PPRE promoter reporter plasmid, followed by treatment of the drug and measurement of luciferase activity.

density Luciferase activity Control group 100 WY-14643 10 μM 300 Comparative Example 1 10 ppm 104 Example 1 10 ppm 124 Example 2 10 ppm 115 Example 3 10 ppm 123 Example 4 10 ppm 128 Example 5 10 ppm 138 Example 6 10 ppm 132

density Luciferase activity Control group 100 TGZ 10 μM 300 Comparative Example 1 10 ppm 104 Example 1 10 ppm 125 Example 2 10 ppm 116 Example 3 10 ppm 124 Example 4 10 ppm 128 Example 5 10 ppm 139 Example 6 10 ppm 132

Table 6 and Table 7 show that when Examples 1 to 6 according to the present invention are used, a significant degree of activity of PPAR? And PPAR? Is induced and exhibits high luciferase activity.

[Test Example 6] Promoting effect of keratinocyte differentiation

In order to examine the differentiation promoting effect of keratinocytes in the samples, the amount of corned envelope (CE) generated upon differentiation of keratinocytes was measured using absorbance.

First, the keratinocytes of the primary culture separated from the epidermis of the newborn baby are placed in a culture flask and adhered to the bottom. After the test substance of Table 8 is treated at a concentration of 1 ppm in the culture medium, % ≪ / RTI > until growth. At this time, low calcium (0.03 mM) and high calcium (1.2 mM) treatment groups were negative control and positive control, respectively. Then, the cultured cells were harvested, washed with PBS (Phosphate buffered saline), and then suspended in 10 mM Tris-HCl buffer (Tris-HCl, pH 7.4) containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) pH 7.4) was added, sonication, boiling, and centrifugation. The precipitate was suspended again in 1 ml of PBS and the absorbance at 340 nm was measured. Separately, a part of the solution after the sonication was taken to measure the protein content and used as a standard in evaluating the degree of cell differentiation. The results are shown in Table 8 below.

Test substance The ability to differentiate in keratinocytes (%) Low calcium (0.03 mM) solution (negative control) 100 High calcium (1.2 mM) solution (positive control) 210 Comparative Example 1 105 Example 1 120 Example 2 134 Example 3 124 Example 4 123 Example 5 121 Example 6 125

As shown in Table 8, it can be seen that Examples 1 to 6 according to the present invention promote the differentiation of keratinocytes to a significant degree.

[Test Example 7] Measurement of effect of restoring skin barrier function and increasing skin moisturizing power in hairless mouse skin

In order to measure the effect of the samples on the recovery of the damaged skin barrier function due to skin damage and the effect on the increase in skin moisturizing ability, the following experiment was conducted.

First, acetone was applied to the mice (Charles River, Japan) at 8 to 10 weeks after birth periodically for 5 days twice a day to induce barrier function of the skin, such as experimental animals, and then to an evaporator TEWL (transepidermal water loss) was measured to determine the transdermal water loss of more than 40 g / m ² / hr. A vehicle (vehicle-treated group) mixed with propylene glycol and ethanol at a ratio of 7: 3 as a test substance to the selected experimental animals and a sample containing 1% each of Examples 1 to 6 at a ratio of 200 μl per 5 cm ² of skin area , And the TEWL was measured at a constant time while continuously applying for 3 days twice a day. The results are shown in Table 9.

The moisture content of the skin was measured simultaneously using a corneometer (Courage Khazaka, Germany). The results are shown in Table 10. At this time, the skin moisture of the untreated group was also measured.

Time (h) 0 6 12 24 48 72 Vehicle 44.3 42.5 38.7 23.5 19.7 16.3 Comparative Example 1 44.5 41.1 38.4 21.1 16.4 14.2 Example 1 44.9 41.4 28.5 15.4 10.4 7.5 Example 2 44.2 40.4 27.3 16.4 11.4 8.5 Example 3 44.5 41.4 27.7 15.2 11.1 7.1 Example 4 44.8 40.9 27.6 15.1 11.5 7.2 Example 5 44.5 41.2 28.1 14.9 10.9 6.1 Example 6 44.1 41.0 27.9 15.6 11.2 6.5

Time (h) 0 6 24 48 Untreated group 100 91 86 81 Vehicle 100 93 88 83 Comparative Example 1 100 97 89 87 Example 1 100 104.5 109.7 113.8 Example 2 100 103.5 107.9 112.4 Example 3 100 105.1 106.8 114.5 Example 4 100 105.6 109.6 121.2 Example 5 100 104.9 109.8 114.1 Example 6 100 104.2 108.9 112.9

As shown in Table 9, it can be seen that the treatment groups of Examples 1 to 6 recovered the barrier damage rapidly. Also, as shown in Table 10, it can be confirmed that the treatment groups of Examples 1 to 6 are high in the amount of skin moisture.

Accordingly, it can be seen that when the ginseng fruit forage extract is treated, the skin moisture content increases as well as the barrier damage recovery. Also, when the extract of ginseng fruit extract is used, the efficacy is better than that of the extract of ginseng fruit product can confirm.

Claims (8)

A cosmetic composition for skin whitening improvement comprising, as an active ingredient, an extract of a ginseng fruit which has been subjected to forage treatment by a milling method,
The ginseng fruit treated with the wheat flour is obtained by putting the fruit of ginseng in honey and absorbing the honey, roasting it at a temperature of 100 to 160 ° C for 10 minutes to 1 hour and then drying it on a shade. Cosmetic composition. [Claim 3] The cosmetic composition for improving skin whitening according to claim 1, wherein the extract of the forage-treated ginseng fruit is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the composition. [Claim 3] The cosmetic composition for improving skin whitening according to claim 2, wherein the extract of the forage-treated ginseng fruit is contained in an amount of 10% by weight based on the total weight of the composition. The composition of claim 1, wherein the composition is selected from the group consisting of softening agents, convergent lotion, nutritional lotion, cleansing foam, cleansing water, essence, pack, powder, body lotion, body oil, lotion, ointment, gel, cream, patch, stick, A cosmetic composition for improving skin whitening characterized by being formulated with a spray agent. Claims 1. A cosmetic composition for anti-aging skin comprising an extract of ginseng fruit which has been subjected to forage treatment by a main-seeding process as an active ingredient,
The cosmetic composition for skin anti-aging according to any one of the above claims, characterized in that the ginseng fruit which has been subjected to forage treatment is obtained by immersing the ginseng fruit in the yellow juice of the container and roasting it for 15 to 30 minutes at a temperature of 100 to 180 ° C. [Claim 6] The cosmetic composition for skin antiaging according to claim 5, wherein the extract of the forage-treated ginseng fruit is contained in an amount of 0.001 to 30.0% by weight based on the total weight of the composition. [Claim 7] The cosmetic composition for skin antiaging according to claim 6, wherein the extract of the forage-treated ginseng fruit is contained in an amount of 10% by weight based on the total weight of the composition. 6. The composition of claim 5, wherein the composition is selected from the group consisting of softening agents, convergent lotion, nutritional lotion, cleansing foam, cleansing water, essence, pack, powder, body lotion, body oil, lotion, ointment, gel, cream, patch, stick, A cosmetic composition for skin aging, characterized by being formulated with a spray agent.