KR20170135775A - A Feed Stuff Additives for farming fish - Google Patents

Abstract

According to an embodiment of the present invention, a feed additive for fishes is composed of 35-40 parts by weight of glutathione, 75-85 parts by weight of beta-glucan, 5-6 parts by weight of B-group vitamins, and 0.01-1 parts by weight of yeast. The feed additive of the present invention promotes metabolism in body, and enhances resistance to diseases in animals by increasing the number of immune factors involved in immune responses, thereby reducing consumption of various antibiotics when injecting or feeding the feed additive while raising animals.

Description

[0001] The present invention relates to a feed additive for farming fish,

Embodiments of the present invention are directed to compositions that are added to feed for aquaculture.

In animal husbandry and fisheries, it is very important to prevent diseases against animals that are used industrially. Antibiotics are prescribed to feed and the like in order to increase the resistance to disease because failure to prevent disease lowers the resistance of animals to disease, productivity is reduced and big economic loss occurs. However, most of the prevention and treatment of infectious diseases of animals are dependent on the use of antibiotics, and thus the expression of resistant bacteria due to abuse of antibiotics causes resistance to existing antibiotics. In addition, the increased resistance to existing antibiotics increases the dose of antibiotics, which causes the problem of residual antibiotics in the animal body.

Therefore, various immune enhancers and metabolism promoters have been developed in order to restore the appetite and vigor of the animals without using antibiotics and to increase the vitality and immunity of animals. For example, Korean Patent Publication No. 2007-0031815 discloses feed additive for pig immunity enhancement including biotite and sulfur.

Korean Patent Publication No. 2007-0031815 (2007.03.20)

It is an object of the present invention to provide a composition capable of promoting vitality and immunity of aquaculture fish and reducing the use of various antibiotics in breeding fish.

In order to accomplish the object of the present invention, a feed additive for fish according to an embodiment of the present invention comprises 35 to 40 parts by weight of glutathione, 75 to 85 parts by weight of betaglucan, 5 to 6 parts by weight of vitamin B group, 0.01 to 1 part by weight of yeast.

According to one embodiment of the present invention, at least one selected from the group consisting of taurine, UDCA, and methionine may be further contained in an amount of 100 parts by weight.

According to one example of the present invention, the group B vitamins may include vitamin B ₁ , vitamin B _2, and vitamin B ₆ .

According to one example of the present invention, the group B vitamins may further include choline and inositol.

In addition, another embodiment of the present invention to achieve the above object, the glutathione 36 parts by weight of beta-glucan to 80 parts by weight of taurine, 100 parts by weight of vitamin B ₁ 1.5 parts by weight of vitamin B ₂ 3.75 parts by weight of vitamin B ₆ 0.9 And 0.01 to 1 part by weight of yeast.

In accordance with at least one embodiment of the present invention, the feed additive for fish according to the present invention promotes metabolism in the body and increases immune factors involved in the immune response to increase the anti-infective ability of the animal, The use of various antibiotics can be reduced.

In addition, the feed additive for fish according to the present invention can be used as a safe feed additive or a medicinal product which can be administered or administered to an animal for a long time due to reduced toxicity to liver and kidney.

The present invention is characterized by containing 35 to 40 parts by weight of glutathione, 75 to 85 parts by weight of betaglucan, 5 to 6 parts by weight of vitamin B group and a trace amount of yeast.

Hereinafter, the present invention will be described in more detail

Feed additives for fish according to the present invention include glutathione, beta-glucan, group B vitamins and yeast.

Betaglucan was produced by culturing Agrobacterium in a fermentation medium containing sugar as a carbon source, ammonium chloride as a nitrogen source, and other minerals. After fermentation is complete, beta-glucan is present in an insoluble state. Since beta-glucan does not dissolve in a neutral solution state, in order to separate beta-glucan from the fermentation broth from the cells, the culture solution is basified to dissolve the beta-glucan and then the cells are removed by centrifugation or filtration. The solution is contacted with a water-soluble organic solvent or the solution is neutralized to precipitate beta-glucan, followed by centrifugation and drying to obtain a purified powder. Beta glucan can also be used in the form of purified or untreated as above. In the examples of the present invention, a powder type purified by the above method was used.

Glutathione is an amino acid polymer, has strong antioxidant ability, improves liver function and helps to decompose toxins in the body. In addition, by removing harmful free radicals and increasing the activity of T lymphocytes and leukocytes by participating in the immune system, it is possible to improve the immunity of the body and to restore the animal's weakened condition due to various chronic diseases. In the composition of the present invention, glutathione can work together with beta-glucan to further enhance immunomodulating ability.

Group B vitamins are water-soluble vitamins other than vitamin C, which can be involved in the energy metabolism process to restore energy and reduce the accumulation of metabolites.

In the present invention, the group B vitamins preferably include vitamins B ₁ , B ₂ and B ₆ . When the composition of the present invention contains vitamin B ₁ , B _2, and B ₆ as a group B vitamin, it enhances energy metabolism of an animal and promotes animal growth, thereby maximizing the metabolism of beta-glucan and glutathione and enhancing immunity . In addition, it can exhibit protective effects on liver and kidney cells.

It is more preferable that the B group vitamins further include choline and inositol. When the composition of the present invention further contains choline and inositol as B group vitamins, the stability to fish can be further improved.

Yeast is widely distributed in the fruit surface of the genus, sap, flower seeds, milk, etc. Especially, it contains a large amount of protein (50 ~ 55%) with excellent amino acid composition and nucleic acid content (RNA 8%, DNA 0.3% ) Is 7 times more than sardine, so yeast itself is a very good health additive and plays a role in suppressing harmful bacteria in the intestines.

If necessary, the composition of the present invention may further comprise at least one selected from the group consisting of taurine, urdecoxycholic acid (UDCA), and methionine. In the case where the composition of the present invention further comprises at least one selected from the group consisting of taurine, UDCA (Ursodeoxycholic acid) and methionine, the metabolic acceleration and immunomodulating effect can be further improved.

In the composition of the present invention, at least one selected from the group consisting of taurine, UDCA (Ursodeoxycholic acid) and methionine may be included in an amount of 100 parts by weight. Within the above range of contents, it is possible to improve the liver function and improve the immunity ability without giving burden to the summoner and the liver.

There is no limitation on the method of administration of the composition of the present invention. For example, it can be administered orally or parenterally.

The feed additive for fish according to the present invention can be produced as an animal feed additive. The animal feed additive of the present invention may further comprise a carrier or stabilizer which is acceptable to mammals, birds and fishes according to a method which can be easily carried out by those skilled in the art. Examples of the carrier or stabilizer include, but are not limited to, vitamins, amino acids, carbohydrates, fats, minerals, antioxidants, antibiotics, antibacterial agents and other additives.

The shape of the animal feed additive of the present invention is not particularly limited and may be, for example, a shape such as powder, granule, pellet, suspension and the like. The animal feed additive of the present invention can be supplied to mammals, birds and fishes singly or in a mixed form.

The feed additive for fish according to the present invention can be manufactured from a pharmaceutical preparation such as an animal medicine. The pharmaceutical preparations of the present invention can be prepared by using at least one selected from the group consisting of pharmaceutically acceptable carriers, diluents and excipients according to a method which can be easily carried out by those having ordinary skill in the art to which the present invention belongs. Or by formulating it into a unit dose form or by inserting it into a multi-dose container. The formulations may be, but are not limited to, animal injections, powders, solutions, granules or tablets. For example, when it is prepared as an animal injectable solution or an oral solution, it can be formulated using distilled water, ethyl oleate, ethanol, propylene glycol, glycerin, etc., and antioxidants such as ascorbic acid, sodium hydrogen sulfite, sodium pyruvate, Tocopherol, and the like, but the present invention is not limited thereto. In the case of an animal powder or granule preparation, it may be formulated using vitamins, glucose, lactose, starch, enzyme, etc., but is not limited thereto.

The pharmaceutical preparations of the present invention can be administered orally or parenterally. In detail, in the case of oral administration, it can be directly administered orally or mixed with drinking water or mixed with feed. In the case of parenteral administration, it can be administered by intramuscular injection method, but is not limited thereto. The dosage of the pharmaceutical preparation of the present invention can be adjusted depending on the kind of animal to be administered, body weight, age and the like, and a suitable dosage can be adjusted by those skilled in the art.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to the following examples. It will be apparent to those skilled in the art that these embodiments are illustrative of the present invention and are not intended to limit the scope of the appended claims and that various changes and modifications may be made to the embodiments within the scope and spirit of the invention , And it is natural that such variations and modifications fall within the scope of the appended claims.

Example

≪ Preparation of feed additives for fishes >

The composition of Table 1 below was mixed with the carrier to prepare 1 kg of the composition.

Example 1 Example 2 Comparative Example 1 Comparative Example 2 Glutathione (g) 36 36 - - Beta Glucan (g) 80 80 80 80 Taurine (g) - 100 100 - Vitamin B ₁ (mg) 1500 1500 - - Vitamin B ₂ (mg) 3750 3750 - - Vitamin B ₆ (mg) 900 900 - -

<Measurement of immune enhancement effect in fish>

In order to confirm the effect of the composition for enhancing immunity according to the present invention, 1 kg / ton of each of the Example 1, Example 2 and Comparative Example 1 of Table 1 were mixed with each of the flounder, urophobic (rockfish), perch and eel And fed for 4 weeks. Fish fed only diets without additional feed additives were used as controls. The fish used in the experiment were collected from blood through the tricuspid veins and loaded into heparinized tubes to measure the immune activity related indicators. The activity of lysozme in blood used as a measure of nonspecific immunity in fish, nitroblue tetrazolium (NBT) activity and the ability to remove active oxygen to measure the activity of phagocytic cells, the number of lymphocytes and neutrophils in blood, The superoxide dismutase (SOD) activity was measured and the results are shown in Table 2 below. In Table 2, the NBT activity was measured at 540 nm and a statistically significant difference (* P &lt; 0.05) was shown separately from each control group.

Fish species
division
Immunity index Lysozyme
(Units / ml) Lymphocyte (%) Neutrophil
(%) NBT
activity SOD
activity
(Units / ml) halibut


Control group 27.2 71.2 22.3 0.52 106.7 Comparative Example 1 30.5 74.6 27.8 0.58 112.4 Example 1 34.2 79.5 32.2 0.64 118.4 Example 2 49.6 * 83.6 * 39.6 * 0.78 * 123.6 * Rockfish


Control group 23.4 77.5 25.8 0.37 70.1 Comparative Example 1 27.5 78.9 31.6 0.42 75.1 Example 1 32.4 81.6 34.1 0.50 81.2 Example 2 36.0 * 84.8 39.4 * 0.61 * 87.1 * bass


Control group 21.8 74.2 24.6 0.49 112.3 Comparative Example 1 25.5 76.7 33.7 0.54 21.6 Example 1 29.7 * 79.4 39.6 * 0.69 * 125.5 Example 2 33.6 * 82.8 * 41.8 * 0.76 * 131.6 * eel


Control group 21.3 65.3 22.7 0.12 85.2 Comparative Example 1 26.5 67.5 27.8 0.17 89.2 Example 1 32.1 * 71.5 30.4 * 0.19 92.2 Example 2 38.2 * 82.9 * 38.1 * 0.25 * 105.49.8 *

Referring to Table 2, it can be seen that when the feed containing the composition of Example 1 or 2, which is the composition of the present invention, was fed, the immunity index value was increased compared to the feed of the control and the feed of Comparative Example 1. Thus, it can be confirmed that the composition of the present invention has an excellent immunity increasing effect in fish.

<Measurement of effect on liver in fish>

In order to confirm the toxicity to the liver of the immunoconjugation composition according to the present invention, 10 mg / kg of each of the Example 2 and Comparative Example 2 of Table 1 were mixed with each of the flounder, urophobic (rockfish), perch and eel diet, Week. Fish fed only diets without additional feed additives were used as controls. Blood samples were taken from the tail vein of the fishes and the aspartate aminotransferase (AST) and alanine aminotransferase (ALT), enzymes involved in liver activity, were measured in heparinized tubes and the results are shown in Table 3 . A statistically significant difference (* P &lt; 0.05) between the experimental group 2 and the experimental group 2 was separately shown.

Fish species
division
Liver enzyme AST (IU / L) ALT (IU / L) halibut Control group 93.5 84.6 Comparative Example 2 89.7 77.8 Example 2 112.4 * 95.3 * Rockfish
Control group 14.8 4.85 Comparative Example 2 13.4 4.68 Example 2 16.4 * 5.12 bass Control group 23.7 7.15 Comparative Example 2 22.4 6.45 Example 2 25.3 * 7.62 * eel

Control group 92.6 8.13 Comparative Example 2 83.7 7.72 Example 2 105.4 * 8.51

The results are shown in Table 3. The results are shown in Table 3. When the feed containing the composition of Example 2 of the present invention was fed, the values of liver enzymes were increased compared to those of the control and the feed of Comparative Example 1. Through this, it can be confirmed that the composition of the present invention enhances the effect of liver activity in fish.

Claims (4)

35 to 40 parts by weight of glutathione, 75 to 85 parts by weight of beta-glucan, 5 to 6 parts by weight of vitamin B group and 0.01 to 1 part by weight of yeast.
The method according to claim 1,
Wherein the composition further comprises 100 parts by weight of at least one member selected from the group consisting of taurine, UDCA (Ursodeoxycholic acid, urushodeoic acid) and methionine.
The method according to claim 1,
Wherein the group B vitamins are vitamin B ₁ , vitamin B _2, vitamin B _6, choline and inositol.
A feed additive for fish comprising 36 parts by weight of glutathione, 80 parts by weight of betaglucan, 100 parts by weight of taurine, 1.5 parts by weight of vitamin B ₁ , 3.75 parts by weight of vitamin B ₂ , 0.9 parts by weight of vitamin B ₆ and 0.01-1 parts by weight of yeast.