KR20230000565A - A Feed Stuff Additives for farming fish - Google Patents

Abstract

The feed additives for fish according to an embodiment of the present invention contains: 34 to 38 parts by weight of glutathione; 77 to 84 parts by weight of beta glucan; 5 to 6 parts by weight of group B vitamins; 0.01 to 1 part by weight of yeast; and 100 parts by weight of at least one selected from a group consisting of taurine, UDCA and methionine, and thus, it is possible to reduce the use of various antibiotics when administered or taken during breeding of animals by promoting metabolism in the body and increasing immune factors involved in the immune response to increase the anti-pathogenicity of animals.

Description

Feed additives for fish {A Feed Stuff Additives for farming fish}

Embodiments of the present invention relate to a composition added to the feed of farmed fish.

In the livestock industry and fishery, the problem of preventing diseases of industrially used animals is very important. If the disease resistance of animals is lowered due to failure to prevent disease, productivity is reduced and large economic losses are incurred. Therefore, antibiotics are prescribed for feed and the like to increase disease resistance. However, since most of the prevention and treatment of animal infections depend on the use of antibiotics, there are problems such as resistance to existing antibiotics due to the expression of resistant strains due to misuse and abuse of antibiotics. In addition, due to the increase in resistance to existing antibiotics, the dosage of antibiotics is increased, and the remaining antibiotics in the body of animals is also a problem.

Therefore, in terms of increasing the vitality and immunity of animals by restoring the appetite and energy of animals without using antibiotics, various immune enhancers and metabolism promoters are being developed. For example, Korean Patent Publication No. 2007-0031815 discloses a feed additive for enhancing pig immunity containing biotite and sulfur.

Korean Patent Publication No. 2007-0031815 (2007.03.20)

One object of the present invention is to provide a composition capable of reducing the use of various antibiotics in breeding fish by enhancing the vitality and immunity of farmed fish.

In order to achieve the above object of the present invention, a feed additive for fish according to an embodiment of the present invention includes 34 to 38 parts by weight of glutathione; 77 to 84 parts by weight of beta glucan; 5 to 6 parts by weight of group B vitamins; 0.01 to 1 part by weight of yeast; and at least one selected from the group consisting of taurine, UDCA and methionine in 100 parts by weight.

According to an example related to the present invention, the group B vitamins may include vitamin B ₁ , vitamin B _{2 ,} vitamin B _{6 ,} choline and inositol.

In addition, another embodiment of the present invention in order to realize the above object, glutathione 36.5 parts by weight, beta glucan 80 parts by weight, taurine 100 parts by weight, vitamin B ₁ 1.5 parts by weight, vitamin B ₂ 3.75 parts by weight, vitamin B ₆ 0.9 Disclosed is a feed additive for fish comprising 0.01 to 1 part by weight of yeast.

The feed additive for fish related to at least one embodiment of the present invention configured as described above promotes metabolism in the body and increases immune factors involved in immune reactions to increase the anti-pathogenicity of animals, and when administered or taken during breeding of animals. can reduce the use of various antibiotics.

In addition, the feed additive for fish according to the present invention has reduced liver and kidney toxicity and can be used as a safe feed additive or medicine that can be administered or taken to animals for a long period of time.

The present invention is characterized by comprising 35 to 40 parts by weight of glutathione, 75 to 85 parts by weight of beta glucan, 5 to 6 parts by weight of group B vitamins, and a trace amount of yeast.

Hereinafter, the present invention will be described in more detail.

The feed additive for fish according to the present invention includes glutathione, beta glucan, group B vitamins and yeast.

Beta glucan was produced by culturing Agrobacterium in a fermentation medium containing sugar as a carbon source, ammonium chloride as a nitrogen source, and other minerals. After fermentation is complete, beta glucan remains in an insoluble state. Since beta glucan is insoluble in a neutral solution state, in order to separate beta glucan from the cells in the fermentation broth, the culture medium is basified to dissolve the beta glucan, and then the cells are removed by centrifugation or a filter. Beta glucan is precipitated by contacting this solution with a water-soluble organic solvent or by neutralizing the solution, followed by centrifugation and drying to obtain a purified powder. Beta glucan can also be used in purified or unpurified form as described above. In the embodiment of the present invention, the powder form purified by the above method was used.

Glutathione is an amino acid polymer that has strong antioxidant power, improves liver function, and helps decompose toxic substances in the body. In addition, by removing harmful active oxygen and increasing the activity of T lymphocytes and leukocytes by being involved in the immune system, it is possible to enhance the body's immunity and restore the energy of animals weakened by various chronic diseases. In the composition of the present invention, glutathione can further improve immunomodulatory ability by acting together with beta glucan.

Group B vitamins are water-soluble vitamins, except for vitamin C, which are involved in the energy metabolism process to restore energy and reduce the accumulation of metabolites.

In the present invention, the group B vitamins preferably include vitamins B ₁ , B ₂ and B ₆ . When the composition of the present invention includes vitamins B ₁ , B ₂ and B ₆ as group B vitamins, it helps to improve energy metabolism of animals and promote animal growth, maximizing the effect of promoting metabolism of beta-glucan and glutathione and enhancing immunity. can make it In addition, it can exhibit protective effects on liver and kidney cells.

More preferably, the group B vitamins further include choline and inositol. When the composition of the present invention further includes choline and inositol as group B vitamins, stability to fish can be further improved.

Yeast is widely distributed in natural fruit surfaces, sap, flower glands, milk, etc., and especially contains a large amount of protein (50 ~ 55%) with excellent amino acid composition, and nucleic acid content (RNA 8%, DNA 0.3%) ) is 7 times more than sardines, so yeast itself is an excellent health additive and plays a role in suppressing harmful bacteria in the intestines.

If necessary, the composition of the present invention may further include at least one selected from the group consisting of taurine, UDCA (Ursodeoxycholic acid) and methionine. When the composition of the present invention further contains at least one selected from the group consisting of taurine, UDCA (Ursodeoxycholic acid) and methionine, the effect of promoting metabolism and immunoregulation can be further improved.

In the composition of the present invention, at least one selected from the group consisting of taurine, UDCA (Ursodeoxycholic acid) and methionine may be included in 100 parts by weight. Within the above content range, it is possible to exhibit the effect of improving liver function and enhancing immunity without burdening the summoning organs and liver.

The method of administration of the composition of the present invention is not limited. For example, it can be administered orally or parenterally.

The feed additive for fish according to the present invention may be prepared as an animal feed additive. The animal feed additive of the present invention may further include a carrier or stabilizer acceptable to mammals, birds, and fish according to a method that can be easily performed by those skilled in the art. The carrier or stabilizer may include, for example, vitamins, amino acids, carbohydrates, fats, minerals, antioxidants, antibiotics, antibacterial agents, and other additives, but is not limited thereto.

The shape of the animal feed additive of the present invention is not particularly limited, and may be, for example, powder, granule, pellet, suspension or the like. The animal feed additive of the present invention may be supplied alone or mixed with feed for mammals, birds and fish.

The feed additive for fish according to the present invention can be manufactured into pharmaceutical preparations such as veterinary drugs. The pharmaceutical preparation of the present invention uses at least one selected from the group consisting of pharmaceutically acceptable carriers, diluents and excipients according to a method that can be easily performed by those skilled in the art. By formulating it, it can be prepared in unit dose form or prepared by inserting it into a multi-dose container. At this time, the formulation may be an animal injection, powder, liquid, granule or tablet, but is not limited thereto. For example, in the case of preparation of animal injections and oral solutions, distilled water, ethyl oleate, ethanol, propylene glycol, glycerin, etc. may be used, and as antioxidants, ascorbic acid, sodium hydrogen sulfite, sodium pyrosulfite, It may further include tocopherol and the like, but is not limited thereto. When prepared as animal powders and granules, it may be formulated using vitamins, glucose, lactose, starch, enzymes, etc., but is not limited thereto.

The pharmaceutical preparations of the present invention may be administered orally or parenterally. Specifically, in the case of oral administration, it can be administered directly orally or by mixing with drinking water or by mixing with feed, and in the case of parenteral administration, it can be administered by conventional intramuscular injection, but is not limited thereto. The dosage of the pharmaceutical preparation of the present invention may be adjusted according to the type, weight, age, etc. of the animal to be administered, and an appropriate dosage may be adjusted by those skilled in the art.

Hereinafter, in order to aid understanding of the present invention, the present invention will be described in more detail based on the following examples. However, these examples are merely illustrative of the present invention and do not limit the scope of the appended claims, and it is obvious to those skilled in the art that various changes and modifications to the examples are possible within the scope and spirit of the present invention. However, it is natural that such variations and modifications fall within the scope of the appended claims.

Example

<Manufacture of feed additives for fish>

1 kg of the composition was prepared by mixing the composition of Table 1 with a carrier.

Example 1 Example 2 Comparative Example 1 Comparative Example 2 Glutathione (g) 36 36 - - Beta Glucan (g) 80 80 80 80 Taurine (g) - 100 100 - Vitamin B ₁ (mg) 1500 1500 - - Vitamin B ₂ (mg) 3750 3750 - - Vitamin B ₆ (mg) 900 900 - -

<Measurement of immune enhancing effect in fish>

In order to confirm the effect of the immune enhancing composition according to the present invention, Example 1, Example 2 and Comparative Example 1 in Table 1 were mixed with halibut, rockfish (rockfish), sea bass and eel feed at an amount of 1 kg per 1 ton, respectively. and paid for 4 weeks. Fish fed only feed without additional feed additives were used as a control group. The fish used in the experiment were collected with blood through the caudal vein and put in a heparinized tube to measure indicators related to immune activity to confirm the immune enhancing effect. The activity of lysozme in the blood used as a measure of non-specific immune ability in fish, the number of lymphocytes and neutrophils in the blood used as an immune indicator in general fish, nitroblue tetrazolium (NBT) activity to measure the activity of phagocytes, and the ability to remove active oxygen To measure superoxide dismutase (SOD) activity was measured and the results are shown in Table 2 below. In Table 2 below, NBT activity was measured at 540 nm, and values showing statistically particularly significant differences (*P<0.05) compared to each control group were marked separately.

fish species
division
immune markers Lysozyme
(Units/ml) Lymphocytes (%) Neutrophil
(%) NBT
activity SOD
activity
(Units/ml) halibut


control group 27.2 71.2 22.3 0.52 106.7 Comparative Example 1 30.5 74.6 27.8 0.58 112.4 Example 1 34.2 79.5 32.2 0.64 118.4 Example 2 49.6* 83.6* 39.6* 0.78* 123.6* Rockfish


control group 23.4 77.5 25.8 0.37 70.1 Comparative Example 1 27.5 78.9 31.6 0.42 75.1 Example 1 32.4 81.6 34.1 0.50 81.2 Example 2 36.0* 84.8 39.4* 0.61* 87.1* bass


control group 21.8 74.2 24.6 0.49 112.3 Comparative Example 1 25.5 76.7 33.7 0.54 21.6 Example 1 29.7* 79.4 39.6* 0.69* 125.5 Example 2 33.6* 82.8* 41.8* 0.76* 131.6* eel


control group 21.3 65.3 22.7 0.12 85.2 Comparative Example 1 26.5 67.5 27.8 0.17 89.2 Example 1 32.1* 71.5 30.4* 0.19 92.2 Example 2 38.2* 82.9* 38.1* 0.25* 105.49.8*

Referring to Table 2, it can be seen that when feeding the feed containing the composition of the present invention Example 1 or Example 2, the immune index value increased compared to the case of feeding the feed of the control group and Comparative Example 1. Through this, it can be confirmed that the composition of the present invention shows an excellent immunity increasing effect in fish.

<Measurement of liver effects in fish>

In order to confirm the toxicity of the immune enhancing composition according to the present invention to the liver, Example 2 and Comparative Example 2 in Table 1 were mixed with halibut, rockfish (rockfish), perch and eel feed at an amount of 10 kg per ton, respectively. paid for a week. Fish fed only feed without additional feed additives were used as a control group. For the fish used in the experiment, blood was collected through the caudal vessel and placed in a heparinized tube to measure the concentrations of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), which are enzymes related to liver activity, and the results are shown in Table 3 below. . In the case of each Example 2 experimental group, a value showing a statistically significant difference (*P<0.05) compared to the Comparative Example 2 experimental group was marked separately.

fish species
division
liver active enzyme AST (IU/L) ALT(IU/L) halibut control group 93.5 84.6 Comparative Example 2 89.7 77.8 Example 2 112.4* 95.3* Rockfish
control group 14.8 4.85 Comparative Example 2 13.4 4.68 Example 2 16.4* 5.12 bass control group 23.7 7.15 Comparative Example 2 22.4 6.45 Example 2 25.3* 7.62* eel

control group 92.6 8.13 Comparative Example 2 83.7 7.72 Example 2 105.4* 8.51

Referring to Table 3, it can be seen that when feeding the feed containing the composition of Example 2 of the present invention, the liver active enzyme value increased compared to the case of feeding the feed of the control group and Comparative Example 1. Through this, it can be confirmed that the composition of the present invention enhances the liver activity in fish.

Claims (3)

34 to 38 parts by weight of glutathione;
77 to 84 parts by weight of beta glucan;
5 to 6 parts by weight of group B vitamins;
0.01 to 1 part by weight of yeast; and
A feed additive for fish, comprising 100 parts by weight of at least one selected from the group consisting of taurine, UDCA and methionine.
According to claim 1,
The B group vitamins include vitamin B ₁ , vitamin B _{2 ,} vitamin B _{6 ,} choline and inositol.
36.5 parts by weight of glutathione, 80 parts by weight of beta glucan, 100 parts by weight of taurine, 1.5 parts by weight of vitamin B ₁ , 3.75 parts by weight of vitamin B ₂ , 0.9 parts by weight of vitamin B ₆ , and 0.01 to 1 part by weight of yeast.