KR20170089798A - Composition comprising filler and botulinum toxin for improving skin wrinkle, ageing or treating neuromuscular disease - Google Patents

Abstract

Disclosed is a composition for treating neuromuscle-associated diseases, delaying aging, and ameliorating wrinkles on skin containing filler and botulinum toxin. According to the present invention, the composition can treat neuromuscle-associated diseases, aging, and skin wrinkles caused by two factors with a single injection, and also brings synergistic effects, thereby reducing costs and the number of injection while increasing user convenience.

Description

TECHNICAL FIELD The present invention relates to a composition for treating skin wrinkles, improvement of aging, or neuromuscular-related diseases comprising a filler and a botulinum toxin,

The present invention relates to the treatment of skin wrinkles, improved aging or neuromuscular related diseases using botox and filler.

Usually facial wrinkles are classified into static rhytides and dynamic rhytides. Static wrinkles are mainly shallow, and due to aging and volume defects of the skin, fillers are used to fill the volume deficit of the tissue for treatment. Dynamic wrinkles are mainly deep, caused by contraction of the facial muscles, and are treated by paralyzing muscles by injecting a botulinum toxin, for example, a product sold under the trade name Botox, into the facial muscles to treat the facial muscles.

Korean Patent Laid-Open Publication No. 2011-0062277 discloses a composition which is prepared by a freeze-drying method and which is improved in stability of an active ingredient for skin care and is not released from the skin even when adhered for a long period of time .

United States Patent Application Publication No. 2009-0196928 relates to a biocompatible hydrogel composition which forms a hydrogel through a crosslinking reaction using an electrophilic substance and a nucleophilic substance and uses the same for releasing an active ingredient such as Botox .

Fillers and botulinum toxins may be used in combination to improve skin wrinkles. Fillers are usually absorbed between 6 months and 2 years. Muscle movement is known to promote absorption, and Botox has a duration of action of about 4-6 months, so repeated injections are needed. In the case where the conventional filler and Botox are used separately, the inconvenience is increased due to repetitive injection, so that they can complement each other's functions and bring about synergy effects. In addition, It is necessary to develop a product with increased convenience.

The purpose of the present invention is to solve the wrinkles and aging of the skin due to two causes by one shot, and to improve the efficacy of one ingredient and the convenience of the other, thereby improving wrinkles, aging and neuromuscular diseases To provide a composition for use in the field.

In one aspect, the present invention provides a composition or cosmetic composition for treating skin wrinkles, improving aging, and / or neuromuscular related diseases comprising a filler and a botulinum toxin. The composition may be used for cosmetic / cosmetic or therapeutic purposes depending on the specific purpose desired in the subject of use. Various fillers known in the art may be used. Short, medium or long-term fillers may be used depending on whether they are persistent or not. For example, hyaluronic acid series, collagen series, calcium hydroxyapatite series or polyacrylamide series Or more filler may be used, but is not limited thereto.

The botulinum toxin may be any known one, for example, but not limited to, Botox®, Medithoxin, Dysport®, Botulex, Myobloc®, BTX or Xeomin®.

The botulinum toxin and filler included in the composition according to the present invention may be included in various ratios, taking into account effects such as the desired effect, e.g. duration, and in one embodiment the filler is included in the range of about 0.01 to 1 ml per 1 ml of composition , And the botulinum toxin can be included in about 1 - 5000 units.

Further, it may include, but is not limited to, about 1 to 5000 units, in particular about 5 to 2500 units, of Botox per ml of filler based on the filler.

The composition according to the present invention solves skin wrinkles, aging, or neuromuscular-related diseases caused by two factors by one injection, and at the same time, enhances the efficacy of one ingredient and improves the efficacy of another ingredient. And the cost is reduced.

Figure 1 shows the factors used in the calculation of SFI (Sciatic Function Index) values.
FIG. 2 is a graph showing the score calculated by dividing the grade according to the degree of deformation of the foot shape and the gait of the rat.
Fig. 3 shows the appearance of the rat's feet at 4, 8, 11, and 13 weeks after injection of 1X Botox control, 5X Botox control, 5X Botox + 0.1ml filler group, 10X Botox + 0.1ml filler group, 25X Botox + 0.1ml filler group And a photograph of a rat observing the shape of a footprint. The standard dose of Botox in rats is defined as 1X, 1X is 6 units / kg on the basis of Allergan Botox and 1.2 units for 200 g of rat body weight.
4 is an analysis of the footprints after the same experiment as in Fig.
5 is an analysis of the SFI value (FIG. 5) after the same experiment as in FIG.
FIG. 6 is a graph showing the results of the 1X Botox control, 5X Botox control, 5X Botox + 0.1ml filler group, 10X Botox + 0.1ml filler group, 25X Botox + 0.1ml filler group analyzed at 4, 8, This graph is a numerical value that shows the grade according to the degree of deformation of foot shape and gait.
Fig. 7 is a graph showing the toxicity of Botox and Filler injected in groups of 250X (3 rats), 50X (3 rats) and 35X (3 rats), 30X (1), 25X (1 mouse) group and the representative rats in each group.

The present invention is based on the discovery that when a filler and a botulinum toxin are administered in a single composition, convenience is enhanced due to the reduction of the number of injections due to the synergy of the effect as well as the long duration of the botox and filler effect.

Accordingly, in one aspect, the present invention relates to a composition for treating skin wrinkles, improving aging, and / or neuromuscular related diseases comprising a filler and a botulinum toxin. The improvement of the skin wrinkles and the aging means improvement of the symptoms of the skin accompanying aging, for example, improvement of skin wrinkles, improvement of skin tone, improvement of skin elasticity and lifting.

Herein, wrinkle refers to all wrinkles of the body, especially facial wrinkles, wherein the facial wrinkles include static rhytides and dynamic rhytides. Static wrinkles are mainly shallow, the cause of which is skin aging and volume deficit. Dynamic wrinkles are mainly deep, caused by contraction of the facial muscles, which are treated by injecting botulinum toxin into the facial muscles to paralyze the muscles.

The botulinum toxin is Clostridium botulinum . C. botryricum, C. baratii and C. argentinense, and toxin secreted by anaerobic bacteria. Botulinum toxin types A and B are purified and used medically. This toxin inhibits the secretion of acetylcholine at the motor nerve endings, which paralyzes the corresponding muscle. Various diseases that can be effective due to these characteristics can be used in the treatment of the present invention. For example, various neuromuscular related diseases such as eyelid seizures, quadriceps (the neck muscles are contracted to cause the neck to move to one side), neck dystonia, rigid eyelid fissure, axillary hyperhidrosis, anal fever, Dysphagia, vocal cord dysfunction, strabismus, chronic neuropathy, dyspepsia, dysmenorrhoea, dysmenorrhoea, dysmenorrhoea, dysmenorrhea, But not limited to, facial muscle hypertrophy (such as masticatory muscles), obturator hypochondriac disorders and benign prostatic hyperplasia as well as wrinkle treatment, rectilinear jaw correction, and skin aging improvement.

The filler is a substance that lifts and removes wrinkles by injecting skin tissue components into the skin wrinkle valley and filling the volume, and hyaluronic acid and collagen are currently used the most. Skin volume is maintained by gel-like intangible substances such as collagen, elastin and hyaluronic acid, which form the skeleton of the dermis.

Various commercially available fillers may be used in the compositions herein, and those skilled in the art will be able to select appropriate ones. For example, the filler may be selected from the group consisting of collagen, hyaluronic acid series (e.g., Restylane, Juvederm, Perfectata, Elravie, Evear, Theosial, etc.), calcium hydroxyapatite (For example, trade name: Sculptra, etc.) and polylactic acid (PLLA) series (for example, trade name: Radius, etc.) , And so on. Depending on the duration, a temporary biodegradable filler (duration of effect of less than one year, for example Restylane, Juvederm, Teosyal, Evolence, etc.), semi-permanent biodegradability Filler (lasting for 1-2 years, for example Reviderm, Matridex, Fascian etc.) and permanent non-degradable filler (lasting effect for 2 years or longer, atelecol, aquamid, vial Colorimide, etc.). Also, it is classified as a voluminizer which increases the volume according to its action mechanism and a stimulator which promotes collagen production after injection.

A variety of commercially available botulinum toxins may be used in the compositions herein, and those skilled in the art will be able to select the appropriate one.

The botulinum toxin herein includes any known botulinum toxin and engineered variant or fusion protein produced by bacteria or produced recombinantly. There are currently seven immunologically distinct serotypes A, B, C, D, E, F and G botulinum toxins, available from Sigma-Aldrich and Metabiologics Inc. (Madison, Wis. And the type of animal that effects it and the symptoms of paralysis induced are different. In particular, Form A and Form B can be used as therapeutic and / or cosmetic preparations. For example, type A may be selected from the group consisting of Botox® (Onabotulinum toxin A, Allergan Inc., USA), Meditoxin (Pacific Pharmaceuticals, Korea), Botulex (Hugel Pharma, Korea), Xeomin® (Merz Pharmaceuticals, USA), Dysport® Ipsen, UK), and type B is MYOBLOC® (Elan, USA), but is not limited thereto.

The botulinum toxin of the present invention may alternatively be a botulinum toxin derivative, i.e., a compound comprising a chemical modification or functional modification as compared to a botulinum toxin having botulinum toxin activity but not modified. For example, the botulinum toxin may have one or more amino acid deletions, alterations, or substitutions compared to the botulinum toxin prior to transformation. For example, the modification may be a botulinum toxin modified in a manner that improves or enhances the characteristics of the botulinum toxin, or reduces side effects. Alternatively, the botulinum toxin can be whole or part thereof having activity and can be used as such or as part of a fusion protein. In addition, the botulinum toxin may be in the form of a precursor of a botulinum toxin, which itself may be non-toxic, for example, a non-toxic zink protease exhibiting toxicity upon degradation by protein hydrolysis.

The compositions according to the present application may be prepared with pharmaceutical or cosmetic compositions. Cosmetic compositions and methods for preparing pharmaceutical compositions are well known in the art and one skilled in the art will be able to select the appropriate techniques relating to manufacture, formulation and additives.

For example, a composition according to the present disclosure may be prepared by mixing the filler and botulinum toxin with a carrier and typically with one or more additional pharmaceutically acceptable aqueous carriers or excipients. Pharmaceutically acceptable carrier or diluent, such as, for example, buffered saline, as well as a topically or cosmetically acceptable carrier or vehicle, including a dermatologically or pharmaceutically acceptable carrier, vehicle or medium. And other ingredients typically used in the composition. The term "dermatologically or pharmaceutically acceptable " as used herein means that the above-mentioned composition or ingredient does not cause excessive toxicity, inadequacy, allergic response, etc. upon skin contact.

The cosmetic composition may comprise, for example, customary adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments and / or flavors.

The cosmetic composition of the present invention may comprise a water-soluble base material comprising an unsaturated fatty acid or propylene glycol including conjugated linoleic acid, linoleic acid, gamma-linoleic acid, alpha-linoleic acid.

In addition, the cosmetic composition of the present invention may be prepared in any form conventionally produced in the art, and examples thereof include a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, But are not limited to, cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray. More specifically, it can be prepared in the form of a flexible lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a hair tonic, a shampoo, a rinse, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the composition of the present invention is a paste, cream or gel, an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc or zinc oxide may be used as a carrier component .

When the composition of the present composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the composition is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, , 3-butyl glycol, glycerol aliphatic ester, polyethylene glycol or fatty acid esters of sorbitan can be used.

When the formulation of the present composition is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the composition of the present invention is an interface-active agent-containing cleansing, the carrier component is selected from the group consisting of aliphatic alcohol sulfates, aliphatic alcohol ether sulfates, sulfosuccinic acid monoesters, isethionates, imidazolium derivatives, methyltaurates, sarcosinates, fatty acid amide ethers Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester. According to a preferred mode of administration, the compositions of the present invention may comprise at least one pharmaceutically acceptable excipient.

In addition, in the cosmetic composition of each of the formulations described above, components other than the above essential components can be selected and mixed without difficulty by those skilled in the art depending on the purpose of use.

The composition according to the invention may also comprise at least one adjuvant selected from the group of pharmaceutical adjuvants known to those skilled in the art, such as thickeners, preservatives, perfumes, colorants, chemical or mineral salts filters, moisturizers, hot spring water and the like. Preservatives commonly used in cosmetics or functional foods are, for example, molecules having antimicrobial activity, such as capryl derivatives (capryloylglycine and glyceryl caprylate), such as hexanediol and sodium levorinate, zinc And copper derivatives (gluconate and PCA), phytosphingosine and derivatives thereof, benzoyl peroxide, pyrrothonol amine, zinc pyrocite and selenium sulfide, econazole, ketoconazole or topical antibiotics such as erythromycin and Clindamycin can be called.

Further, if desired, the composition may further comprise any ingredient conventionally used in cosmetics or in the treatment of neuromuscular related disorders such as the improvement of skin aging such as wrinkles.

In terms of formulations, the compositions may include solutions, emulsions, suspensions, creams, lotions, gels, powders or other solid or liquid compositions typical of the skin suitable for use with the composition.

The proportions of each component in the composition comprising the filler and the botulinum toxin of the present invention can be, for example, about 1 to 5000 units of Botox (based on Allergan botox) per ml of filler, but are not limited thereto, Or the appropriate amount and ratio may be selected in consideration of the severity of the condition requiring treatment, the condition of the patient, the duration of treatment, and the like.

In another embodiment, the present invention also relates to a sustained release formulation comprising Botox as an active ingredient, wherein the composition comprising a filler and a botulinum toxin of the present invention in combination comprises one injection to solve two causes, There is an effect of rising and sustaining. Fillers are absorbed between about 6 months and 2 years, muscle movement is known to promote absorption, and Botox has a duration of action of about 4-6 months, so repeated injections are needed. However, since the composition of the present invention suppresses the movement of the facial muscles, the survival period of the filler can be increased. In the case of botulinum toxin, the effect is prolonged because the filler is slowly absorbed and released. In conclusion, the composition of the present invention has an additional synergistic effect over the additive effect, which reduces the number of injections, thereby enhancing the patient's convenience, as well as reducing side effects and reducing the cost.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the following examples.

Example

Example 1: Preparation of mixed composition of botox and filler

Sprague-Dawley rats (6 weeks old, 0.2 kg, Orient Bio) were used for animal experiments. Botox used in the experiment was dissolved in 0.6 ml of saline solution (200 units / vial, Botulax, Hugel) . The filler was a product of Hughes (Korea). 200 μl of Botox solution was mixed with 100 μl of filler. The standard dose of Botox used in the SD rats was determined as "1X (6 units / kg for allergen botox and 1.2 units for 200 g of rat)" (Jianjun Ma et al. Time course of recovery of juvenile skeletal muscle after Botulinum toxin a injection. Am.J.Phys . Med. Rehabil .Vol.83, No.10), animals the control group 1X botox, 5X botox control, 5X botox +0.1 ml filler group, 10X botox +0.1 ml filler Group, and 25X Botox + 0.1ml filler group. The ratios of botox and filler injected into the animals are summarized in Table 1 below.

* [Table 1]

The total volume of the mixture of botox and botox + filler injected into the rats was used as 300 μl. Anesthetized rats were injected into the right gastrocnemius muscle.

An evaluation of the effects of botox and filler with the footprint analysis and the Sciatic Function Index (SFI) to observe the foot shape and footprint of the rat at 4, 8, 11, and 13 weeks after injection was performed as described previously CJ et al. Transplantation of bone marrow stromal cells for peripheral nerve repair. ExpNeurol 2007; 204: 443-53).

In summary, after applying ink to the feet of a rat, he walked several times in white paper to obtain footprints. The length between the third toe and heel is PL, the length between the first and fifth toes is TS, and the length between the second and fourth toes is called ITS. The SFI values were obtained by substituting the right footprints (EPL, ETS, EITS) and the normal left footprints (NPL, NTS, NITS) 2007; 204: 443-53). SFI = -38.3 x (EPL-NPL) / NPL + 109.5 x (ETS-NTS) / NTS + 13.3 x EITS-NITS / NITS-8.8.

Example 2: Analysis of effect of mixed composition of botox and filler

The results of the analysis by the method of Example 1 are shown in Figs. The SFI value is close to 0 when the gastrocnemius function of the rat is normal, and the closer to -100, the more the total malfunction of the gastrocnemius. In this experiment, the closer the value of SFI is to -100, the better the effect of Botox. After 13 weeks, the effect of Botox was the highest at 4 weeks in all groups. The effect of Botox decreased with time, and the group injected with Botox + Filler showed a slower SFI than that injected with Botox alone And the effect of Botox was longer lasting. Specifically, in the 1X Botox control group, the SFI value was -9.5 (normal SFI about -8.8), which was almost normal Rat level at the 8th week, and the SFI value was 72.74% at the 13th week in the 5X Botox control group, (From -63.86 to -17.41), indicating that the effect of Botox decreased rapidly. On the other hand, the SFI values of the 5X Botox + 0.1ml filler group, 10X Botox + 0.1ml filler group and 25X Botox + 0.1ml filler group increased 62.56% (-61.79, -23.13), an increase of 55.17% (-61.14 to -27.41) and a 56.53% increase (-89.29 to -38.81). This shows that the effect of Botox is maintained higher at 13 weeks compared to the 1x, 5X Botox control in these three experimental groups (see Figures 3, 4 and 5).

Grades 1 and 2 (see FIG. 2) were made according to the degree of deformation of the right foot of each group injected with Botox or Botox + filler, and scores were calculated. In this experiment, the higher the score of the foot deformity (evaluation table 1) of the rat, the more uneven the stepping (evaluation table 2), and the higher the score, the higher the effect of botox and filler. . After injecting Botox or Botox + Filler and grading it to 4 ~ 13 weeks, we evaluated the results of evaluation by using 25X Botox + 0.1ml filler group with filler added at 13 weeks, 10X Botox + 0.1ml filler group, 5X Botox + 0.1ml Filler group showed more deformation of foot shape than 1X, 5X Botox control group and 25X botox + 0.1ml filler group got the highest score in the evaluation. At 4 weeks in all groups, the foot deformity was the most severe and high grade, and the grade was lowered over time. The 1X, 5X Botox control group was assessed at Grade evaluation 1 (Foot shape) The normal grade (ie, 0 point), while the 25X Botox + 0.1ml filler group showed significant foot deformations at 13 weeks and maintained 2 points (highest score was 3 points) (see FIG. 6).

This effect indicates that the effect of Botox is maintained for a long period of time in comparison with the use of Botox alone in a mixed composition of filler and Botox, and shows an additional synergistic effect of an additional phase due to the combination of botox and filler.

Botox and filler were injected in groups of 250X (3 rats), 50X (3 rats) and 35X (3 rats), and 30X (1 rat), 25X ), And 10X (one mouse) group after Botox injections. All of the mice died except 10X Botox alone group (see Table 2, FIG. 7). In particular, the 25X Botox group was killed, but the rats that received the 25X Botox + 0.1ml filler were normally kept in good condition because the Botox toxin was mixed with the filler to slowly release the toxin .

 [Table 2]

While the present invention has been described in connection with what is presently considered to be the preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, It belongs to the scope of right.

All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. The contents of all publications referred to herein are incorporated herein by reference.

Claims (8)

A pharmaceutical or cosmetic composition for improving skin wrinkles or skin aging comprising (i) hyaluronic acid or polyacrylamide as a filler, and (ii) a botulinum toxin.
2. The composition of claim 1, wherein the botulinum toxin is of the A or B type.
2. The composition of claim 1, wherein the filler and botulinum toxin are administered in a single shot.
4. The composition of claim 1 comprising from 1 to 5000 units of a botulinum toxin per ml of filler.
2. The composition of claim 1 comprising up to 300 units of botulinum toxin per ml of filler.
2. The composition of claim 1, comprising from 1 to 300 units of a botulinum toxin per ml of filler.
7. The composition of claim 1 comprising 6 to 30 units of a botulinum toxin per ml of filler.
The composition of claim 1, wherein the effect of the botulinum toxin is maintained for at least six months by the filler.

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