KR20170078335A - Miticides composition for smokescreen and metnod of exterminating bee mite with the same - Google Patents
Miticides composition for smokescreen and metnod of exterminating bee mite with the same Download PDFInfo
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- KR20170078335A KR20170078335A KR1020150188735A KR20150188735A KR20170078335A KR 20170078335 A KR20170078335 A KR 20170078335A KR 1020150188735 A KR1020150188735 A KR 1020150188735A KR 20150188735 A KR20150188735 A KR 20150188735A KR 20170078335 A KR20170078335 A KR 20170078335A
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- smoke film
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
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Abstract
The bee mite remedy smoke solution of the present invention and the honey bee mite remedy using the same are effective ingredients which are 5-methyl-2- (methylethyl) phenol or a salt thereof acceptable as an animal medicine; And smoke oil, to suppress or alleviate the occurrence of mites in an effective and safe manner.
Description
The present invention relates to a film-forming solution for suppressing or alleviating the occurrence of mites in an effective and safe manner and a method for controlling the same.
In the beekeeping industry, mite disease is occurring worldwide and is a disease of bees that has begun to appear in Europe since the 1970s and is now the most problematic. In particular, bees do not have their own defense mechanisms against the mites, and are suffering from this disease at each stage of growth. Bee mites, the cause of disease, are rapidly spreading throughout the world.
Therefore, there is a need for a method for controlling mites that can be easily applied to a bee farm without worrying about toxicity, while minimizing the occurrence of bee mites and controlling the honey bees' honey.
It is an object of the present invention to provide a smoke film solution for suppressing or alleviating the occurrence of mites in an effective and safe manner and a method of controlling the same.
In order to achieve the above object, the bee mite relief smoke solution according to an embodiment of the present invention is allowed to be used as 5-methyl-2- (methylethyl) phenol or its animal medicine Active ingredient; And smoke oil.
The bee mite relief smoke film solution may contain the active ingredient and the smoke film oil at a weight ratio of 1: 8 to 28.
The bee mite relief smoke film solution may further include any one of fragrance additives selected from the group consisting of lemon flavor oil, citrus flavor oil, grapefruit flavor oil, and combinations thereof.
The bee mite relief smoke film solution may be composed of the active ingredient, the oil for smoke film, and the flavor additive.
The fragrance additive may be included in an amount of less than 2 parts by weight based on 100 parts by weight of the solution consisting of the effective component and the smoke film oil.
The bee mite relief smoke film solution may have a specific gravity of 0.84 to 0.9.
The honey bee mite remedy method according to another embodiment of the present invention is characterized in that the bee mite remedy method comprises the steps of: 1) extracting 5-methyl-2- (methylethyl) phenol or its active ingredient , And 2) preparing a bee mite relief smoke film by injecting a bee mite relief smoke film solution containing a smoke film oil into a smoke film beaker; And a spraying step of spraying the bee mite relief smoke film on the beehive with a smoke film.
The injection in the injection step may be injected through the beehive inlet for 3 to 10 seconds.
The spraying step may be repeated at intervals of 2 to 4 days for a total of 10 to 28 days.
The bee mite relief smoke film solution may contain the active ingredient and the smoke film oil at a weight ratio of 1: 8 to 28.
The bee mite relief smoke film solution may further include any one of fragrance additives selected from the group consisting of lemon flavor oil, citrus flavor oil, grapefruit flavor oil, and combinations thereof.
The bee mite relief smoke film solution may be composed of the active ingredient, the oil for smoke film, and the flavor additive.
The fragrance additive may be included in an amount of less than 2 parts by weight based on 100 parts by weight of the solution consisting of the effective component and the smoke film oil.
The bee mite relief smoke film solution may have a specific gravity of 0.84 to 0.9.
Hereinafter, the present invention will be described in more detail.
Bee mite is a very dangerous parasite that can wipe out a whole population in beekeeping in a few years. This pathogen is harmful to the world, because it makes the bee's blood suck in, weakens the body and easily catches other diseases such as acute paralysis virus disease.
The original host of bee mite was Asian honeybee ( Apis cerana ) was a parasite that survived together without killing or damaging bees directly in the Bongun. This mite propagated to Europe in the 1970s and became the pest which had the greatest influence on the Western honeybee ( Apis mellifera ) at present. The Western honeybee does not have a natural defense mechanism against this pest, And it is a honey bee disease that is being questioned globally. In addition, the main attack target of mite is hematocyte, which is infected from the larva stage of the bee, and the mite grows along with the growth of the bee. Therefore, the treatment is very difficult and in some cases, it affects the growth of bee itself. It can not be done.
The larvae of infected bees are inhibited from normal growth by the mite consumed by feeding the bee's blood. If infected with mite seriously, malformations such as shortening of the body of bees or aquaculture and disappearance of wings will appear. Also, if the born bees have this deformed form, they are naturally withdrawn from the proletariat. On the other hand, it is difficult to distinguish mites infected with multi-grown bees because they are hidden in the ventral space of bees. For this reason, mite infections are easily overlooked.
The inventors of the present invention, applied to bee mites remedy using bee mites relief smoke solution of the invention and yet this by applying an active ingredient of insecticides mites less effective bee mites (Varroa throughout bonggun jacobsoni ) infection and can minimize the effect on bees and honey. Thus, the present invention has been completed.
The bee mite remedy smoke solution according to one embodiment of the present invention is an effective ingredient which is 5-methyl-2- (methylethyl) phenol or its pharmaceutically acceptable salt; And smoke oil.
The smoke film oil may be a mineral oil, an organic solvent or a mixture thereof. The softening oil is preferably a mineral oil.
The oil for smoke film and the active ingredient are mixed to be contained in the smoke film solution, and the smoke film oil is preferably applied as a method of smoke film spray using a smoke film as in the bee mite remedy method described below. In this case, Sized fine particles, which can help to deliver the active ingredient evenly to the honeycomb and bee while floating in the air in the form of smoke for a relatively long time.
Specifically, the active ingredient circulates in the beehive together with the flow of air in the form of a smoke-screen. In this process, the active ingredient acts on the bees or mites in the beehive. The smoke particles have a very fine size Due to its long staying property in the air, the above-mentioned effective ingredient can act evenly on bees and bees larvae closely packed in a small space and inside the beehive, and can be directly sucked into the mites or through the lymph vessels of the bees ingesting the above- The effective ingredient acts on the mite and a relief effect can be obtained.
The bee mite relief smoke film solution may contain the active ingredient and the smoke film oil at a weight ratio of 1: 8 to 28. When the smoke film oil contains more than 28 parts by weight based on 1 part by weight of the active ingredient, it may be difficult to obtain a sufficient anti-misting effect because the content of the active ingredient is small. When the smoke film oil is used in an amount of 1 part by weight If it is contained in an amount of less than 8 parts by weight, the active ingredient may be used in an excessive amount, which is disadvantageous from the viewpoint of economical efficiency.
The bee mite relief smoke film solution may contain the active ingredient and the smoke film oil at a weight ratio of 1:12 to 22, and when the effective ingredient and the smoke film oil are mixed in the weight ratio range, Excellent effects can be obtained in terms of both effects.
The bee mite relief smoke film solution may contain the active ingredient and the smoke film oil at a weight ratio of 1:14 to 18. When the content of the smoke film oil based on the active ingredient 1 is less than 14 parts by weight It is possible that some scooping of the bees may occur immediately after the spraying of the smoke, and in the case of exceeding 18 parts by weight, the effect of increasing the controlling effect by the increase of the effective ingredient content may be insignificant.
Most preferably, the bee mite remedy smoke film solution may contain the active ingredient and the smoke film oil at a weight ratio of 1:15 to 16, and in this case, the effective honey bee parasite control effect of 90% or more is obtained and the bee stability is improved And the amount of the active ingredient remaining on the honeycomb after the control is minimized.
The bee mite relief smoke film solution may further contain less than 5 parts by weight of fragrance additives and less than 2 parts by weight based on 100 parts by weight of the total amount of the active ingredient and the smoke oil.
The fragrance additive may be any one selected from the group consisting of lemon oil, lemon flavor oil, grapefruit oil, grapefruit flavor oil, citrus oil citrus flavor oil, green tea oil, green tea flavor oil, coffee oil, When such a natural oil or natural fragrance oil is applied, the fragrance of the smoke film solution itself can be purified by being well mixed with the smoke film solution.
The bee mite relief smoke film solution may further contain 0.0001 to 0.7 part by weight of the flavoring additive based on 100 parts by weight of the sum of the effective ingredient and the smoke film oil. In this case, An advantageous effect by the fragrance additive can also be obtained without interfering with the effect.
The bee mite relief smoke film solution may be composed of the effective ingredient, the smoke film oil, and the flavoring agent. Specifically, the bee mite remedy smoke film solution may contain 8 to 28 wt% And from 0.00001 to 1.45 parts by weight of the fragrance additive; 12 to 22 parts by weight of the oil for smoke film based on 1 part by weight of the active ingredient, and 0.00001 to 0.38 part by weight of the fragrance additive; 14 to 18 parts by weight of the oil for smoke film based on 1 part by weight of the active ingredient, and 0.00001 to 0.12 part by weight of the fragrant additive; 15 to 16 parts by weight of the oil for smoke film based on 1 part by weight of the active ingredient, and 0.05 to 0.11 part by weight of the flavoring agent. When the bee mite rescue smoke film solution is formed at such a ratio, the stability of the smoke film solution can be maintained for a long period of time, and it is easy to form a fine particle film, and the bees and bees ), It is possible to obtain an effective mite control effect.
The bee mite relief smoke film solution may have a specific gravity of 0.84 to 0.9 and a specific gravity of 0.84 to 0.87. When the bee mite relief smoke film solution has such a specific gravity, the spraying process in the smoke film mode can be more stably carried out using the smoke film machine, the smoke having a small particle size of 0.1 to 10 microns can be manufactured, And it can reach the corners of complex honeycomb and bees evenly as compared with the other application such as spraying or aerosol spraying. Therefore, it is possible to minimize insecticidal effects by minimizing the amount of drug used have.
Wherein the bee mite remedy smoke solution is an effective ingredient which is 5-methyl-2- (methylethyl) phenol [5-methyl-2- (methylethyl) phenol] or its pharmaceutically acceptable salt; And a composition for smoldering oil may be prepared. When the perfume additive is further mixed with the perfume additive, the perfume additive may be mixed together. If it is difficult to mix the perfume additive and the screening oil, if necessary, the perfume additive may be dissolved in the organic solvent, And a method of mixing with an oil for use may be applied. If necessary, insoluble remnants or solid matter can be removed by passing the smoke film solution prepared by the mixing method through a filter.
The honey bee mite remedy method according to another embodiment of the present invention is characterized in that the bee mite remedy method comprises the steps of: 1) extracting 5-methyl-2- (methylethyl) phenol or its active ingredient , And 2) preparing a bee mite relief smoke film by injecting a bee mite relief smoke film solution containing a smoke film oil into a smoke film beaker; And a spraying step of spraying the bee mite relief smoke film on the beehive with a smoke film.
In the case of applying the smoke film solution to the beehive by spraying the smoke particles in the form of a dispersion of the active ingredient in the smoke film oil, the effective ingredient is circulated along with the flow of the air in the smoke state, After the action, the remaining component can be discharged, and the active ingredient in the smoke film moving together with this air flow can act on the mite in the form of remaining in the mud itself or in the beehive, The honeybees act on the mite and may have a controlling effect.
Specifically, in the case of the smoke film spraying method, a smoke film solution having passed through a combustion chamber maintained at a high temperature of about 400 to 600 ° C. is blown out in the form of smoke, and small smoke particles of submicron size carry the active ingredient, The size of the particles is about 0.1 to 10 microns, which is considerably smaller. In the case of applying the above-described smoke film spraying method, since the particle size is smaller and the time for staying in the air is longer than that in the case of applying general spraying or fogging method, a complicated structure of a beehive and bees densely packed in the beehive are mixed Enough delivery of active ingredients is possible to the deep beehive and fine gaps.
In other words, when such effective ingredient is injected into the beehive in the form of a film spray, a relatively uniform control effect can be obtained as a whole from the deep hollow space of the beehive, and the insecticidal effect can be exhibited on the hidden mites. In addition, the efficacy component remains in the beehive due to the oil for smoke film, and it is possible to exhibit the relieving effect on the beads which are not located in the beehive at the time of the spraying of the smoke.
The active ingredient exhibits a remedial effect on mite, but does not exhibit insecticidal effect on bees or bee larvae or pupa, so that it is possible to effectively and selectively prevent mite without affecting or minimizing the effect on products such as bees and honey.
The injection in the injection step may last for 3 to 10 seconds and may last for 4 to 5 seconds. The spraying may be carried out by spraying the smoke film through the inlet of the beehive. The duration of the spraying may vary depending on the size of the beehive, but it is preferably 3 to 10 seconds, preferably 4 to 5 seconds. If the duration of the spray is too long, a large amount of smoke solution may be injected and the smoke may escape in accordance with the flow of air, resulting in waste of the smoke solution. If the duration of the spray is too short, There is a possibility that the smoke film solution may not be sprayed sufficiently.
The mite remover smoke mover may be spraying the bee mite remedy smoke solution at 0.2 g or more per second, or may be sprayed at 0.2 g to 1.2 g per second, and may be sprayed at 0.3 g to 0.7 g per second. Smoothed spraying proceeds with such an injection amount, and smoothed spraying can be stably performed with small particles of 0.1 to 10 microns.
Since the active ingredient is sprayed together with the oil for smoke film and the active ingredient is sufficiently dispersed to the corners in the beehive, the excellent controlling effect can be obtained even by spraying only once. However, in order to exterminate the mites larvae in order to prevent the recurrence of the disease, it is recommended to perform several times with a certain time interval.
The spraying step may be repeated for a total of 10 to 28 days at intervals of 2 to 4 days, and repeatedly for 14 to 21 days at intervals of 2 to 4 days. As a result of repeated experiments by the inventors of the present invention regarding the interval and duration of the smoke film spraying, it is preferable to continuously apply the smoke film solution for a period of 10 to 28 days or more in order to sufficiently control the mite, To eradicate mites infected with the condition, it may be effective to apply continuously for 14 to 21 days.
In addition, the inventors of the present invention have also confirmed that the method of spraying the smoke is efficient in order to enable the active ingredient to be evenly contacted inside the beehive. In the present invention, since the active ingredient is dispersedly applied to the smoke oil, It is possible to obtain a bee honey tick relief effect sufficient even if the spraying step is applied at least twice a week at intervals of 2 to 4 days.
Wherein the smoke film oil contains at least one of an application amount of the effective component and the smoke film oil in the bee mite relief smoke film solution, a content and a kind of the flavor additive that can be further contained in the bee mite relief smoke film solution, And the weight of the contents overlap with those described above, so the description thereof is omitted.
The honey bee mite relief smoke film solution of the present invention and the remedy method using the honey bee mite remedy can be applied to the honey bee honey bee honey bee honey bee honey bee honey bee honey bee honey bee honey bee honey The present invention provides a remedy method capable of obtaining a control effect evenly over the beehive as a whole while minimizing the residual problem of the beehive. By using the smoke film solution or remedy method of the present invention, it is possible to obtain a remedial effect of bee ticks of 86% or more or 90% or more while minimizing the influence on the bees and the application amount of the active ingredient.
The honey bee mite remedy solution of the present invention and the honey bee mite remedy method using the honey bee mite remedy solution have excellent mite remedy rates of 85% or more, but they do not cause or can ignore the behavior of honey bees, and the residual degree of honey, honey, The present invention can provide a bee mite relief smoke film solution capable of relieving mites against the entire life cycle of bees including bee larvae, and a bee mite remedy method using the same.
Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
Experimental Example 1. Manufacture of bee mite control smoke film solution
(Example 1) 5.00 kg of 5-methyl-2- (methylethyl) phenol and 0.50 kg of lemon oil were added to mineral oil, and the mixture was stirred. Then, mineral oil was added to the mixture to obtain 79.13 kg of total mineral oil. 84.63 kg of smoke film solution was prepared.
Example 2 2.50 kg of 5-methyl-2- (methylethyl) phenol and 0.50 kg of lemon oil were added to a mineral oil, and the mixture was stirred. Then, mineral oil was added to the beaker so that the total mineral oil content was 80.32 kg. 83.32 kg of smoke film solution was prepared.
(Example 3) 10.00 kg of 5-methyl-2- (methylethyl) phenol and 0.50 kg of lemon oil were added to mineral oil, and the mixture was stirred to add 76.75 kg of total mineral oil to the honey bee mite remover 87.25 kg of the film solution was prepared.
(Example 4) 20.00 kg of 5-methyl-2- (methylethyl) phenol and 0.50 kg of lemon flavor oil were added to mineral oil, and the mixture was stirred. Then, mineral oil was added to the beaker so that total mineral oil content was 74.51 kg. 95.01 kg of smoke film solution was prepared.
Experimental Example 2. Bee effect remediation test of smoke solution
The beverage mite solution of the present invention was applied to a beehive tank by a method of injecting a bee mite relief smoke film solution of Examples 1 to 4 into a vaporizer type smoke film maker and spraying the smoke at the beehive inlet for 4 to 5 seconds, And the number of bee mite remedies for 29 days was evaluated. In this case, the applied bee was applied to bees of the Italian system which was used for 10 years of domestic beekeeping, and each example was applied to 3 beehives and the average was calculated to evaluate the effectiveness of bee tick extermination. The results are shown in Table 1 . In addition, three beehives without the bee mite remedy smoke solution of Example of the present invention were prepared as the control group, and the bee mite remediation effect was evaluated under the same conditions as in the examples.
Example 1
Example 2
Example 3
Example 4
group
Referring to Table 1 above, all of Examples 1 to 4 showed excellent insecticidal rates as compared with the control group. In the case of Example 2 in which the application amount of the effective ingredient was relatively small, the insecticidal rate was about 53 to 59%, but in Examples 1, 3 and 4, the insecticidal rate was found to be 86% or more I could. On the other hand, the insecticidal rate of Example 1 was higher than that of Example 3 or 4, and it was evaluated that the best remediation efficiency was obtained by applying the optimum mixture ratio of the active ingredient and the smoke oil.
Experimental Example 3. Bee abnormal behavior result when applied to bee mite remedy smoke solution
In the same experiment as Experimental Example 2, when the bee mite relief smoke film solution of the present invention was applied to a smoke machine and applied to a beehive, it was determined whether or not an abnormal behavior occurred to bees on the day of starting the drug, 2 days, 7 days after the start of the administration, and 14 days after the start of the administration. The results are shown in Table 2 below.
Bongun
Example 1
Example 2
Example 3
Example 4
group
Referring to the above Table 2, in the case of Examples 1 and 2, no abnormality such as disturbance, escape, and docking of bees was observed in the test swab, which was the same as that of the control group. In the case of Example 3 and Example 4, it was observed that a partial swelling phenomenon appeared on the day of the administration and one day after the start of the administration, but it was confirmed that no abnormality appeared after 2 days from the start of the administration.
Experimental Example 4. At the beekeeper's house Results of Clinical Experiments
A beekeepers, B beekeepers, and C beekeepers all performed clinical trials of bee mite relief smoke film solutions. Each of the four beehives was tested using eight beehives each having a beehive, and four of the beehives were administered with the smoke film of Example 1 using a softener, and the remaining four of them were used as a non-treated control group, .
Observations on the effects of mite relief were observed for 28 days after the start of dosing. The number of dead mites were examined on 6, 6, 7, 14, 21 and 28 days after dosing. . The number of bees mite leaves that were not rescued even after application of the smoke film solution of the present invention was determined by spraying with about 100 ml of ethyl ether for 5 to 10 minutes and then counting the number of honeybee mite that was shaken off in 95% ethanol of an anesthetized bee. The results are summarized in Table 3 below.
Insecticide number
Caryopsis
Insecticide rate (%)
Insecticide number
Caryopsis
Insecticide rate (%)
Insecticide number
Caryopsis
Insecticide rate (%)
Referring to Table 3 above, when the smoke solution of Example 1 of the present invention was applied to bees of real beekeepers, it was confirmed that the effect of mather than 90% in all three beekeepers was high.
In addition, when applied to three beehive beehives subjected to a clinical test by applying the bee mite relief smoke film solution of the present invention to a smoke film, whether or not an abnormal behavior occurs to bees is determined on the day of the start of the administration, 2 days after the start of the administration, 7 days after the start of the administration, and 14 days after the start of the administration, respectively, and the results are shown in Table 4 below.
As shown in Table 4 above, it was confirmed that no abnormalities such as disturbance, escape, and dipping of bees were observed in all of the three beekeepers by applying the smoke film solution of Example 1 of the present invention, Which means that the smoke film solution of the present invention was not affected.
Experimental Example 5. Evaluation of long-term preservation of smoke film solution
In order to evaluate the long-term preservability of the bee mite relief smoke film solution of the present invention, the smoke film solution of Example 1 prepared in Experimental Example 1 was stored for 24 months at 25 ° C and 60% RH, And the amount of the contents were changed. The results are shown in Table 5 below.
Three or more samples of the smoke film solution of Example 1 were each prepared, and the characteristics of the smoke film were evaluated immediately after production, after 3 months of manufacture, after 6 months, 9 months, 12 months, 18 months, The contents and contents of the active ingredients were tested and the results are shown in Table 5.
The confirmation of the properties was judged to be suitable or unsuitable by checking whether the liquid was a yellowish to yellowish liquid as a result of visual observation. The confirmation experiment was carried out by comparing whether the peaks of the sample solution have the same retention time, . The content of the standard solution and the sample solution were compared using the high-performance liquid chromatography, and the content of the standard solution and the sample solution was evaluated to determine whether the content was within the error range (90 to 110%) of the content of Example 1. In addition, the content test was carried out according to the "content test method" in the General Test Methods for Veterinary Drugs.
number
Immediately
3 months
6 months
9 months
12 months
18 months
24 months
exam
exam
exam
Referring to the results of Table 5 above, it was evaluated that the smoke film solution of the present invention was stable even after being stored at room temperature for 24 months.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.
Claims (11)
Wherein the bee mite relief smoke film solution contains the active ingredient and the smoke film oil at a weight ratio of 1: 8-28.
Wherein the bee mite remedy smoke film solution further comprises any one flavor additive selected from the group consisting of lemon flavor oil, citrus flavor oil, grapefruit flavor oil, and combinations thereof.
Wherein the fragrance additive is contained in an amount of less than 2 parts by weight based on 100 parts by weight of the solution comprising the effective component and the smoke film oil.
Wherein the bee mite relief smoke film solution is composed of the active ingredient, the smoke film oil, and the flavor additive.
The bee mite relief smoke film solution has a specific gravity of 0.84 to 0.9.
A spraying step of spraying the bee mite relief smoke film onto the beehive with a smoke film; / RTI > The bee tick remedy method of claim 1,
Wherein the spraying in the spraying step is sprayed through the beehive inlet for 3 to 10 seconds.
Wherein said spraying step is repeated for a total of 10 to 28 days at intervals of 2 to 4 days.
Wherein the bee mite relief smoke film solution contains the effective ingredient and the smoke film oil at a weight ratio of 1: 8 to 28.
Wherein the bee mite relief smoke film solution has a specific gravity of 0.84 to 0.9.
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| Application Number | Priority Date | Filing Date | Title |
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| KR1020150188735A KR20170078335A (en) | 2015-12-29 | 2015-12-29 | Miticides composition for smokescreen and metnod of exterminating bee mite with the same |
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| KR1020150188735A KR20170078335A (en) | 2015-12-29 | 2015-12-29 | Miticides composition for smokescreen and metnod of exterminating bee mite with the same |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102024124B1 (en) | 2019-07-01 | 2019-09-23 | 주식회사 한국양봉산업 | pest control composition for honeybee mite |
| KR102104045B1 (en) | 2019-09-11 | 2020-04-23 | 주식회사 한국양봉산업 | pest control composition for honeybee mite using bee venom |
| CN115997763A (en) * | 2022-12-14 | 2023-04-25 | 江西农业大学 | Composition for preventing and treating bee mites |
-
2015
- 2015-12-29 KR KR1020150188735A patent/KR20170078335A/en not_active Application Discontinuation
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102024124B1 (en) | 2019-07-01 | 2019-09-23 | 주식회사 한국양봉산업 | pest control composition for honeybee mite |
| KR102104045B1 (en) | 2019-09-11 | 2020-04-23 | 주식회사 한국양봉산업 | pest control composition for honeybee mite using bee venom |
| CN115997763A (en) * | 2022-12-14 | 2023-04-25 | 江西农业大学 | Composition for preventing and treating bee mites |
| CN115997763B (en) * | 2022-12-14 | 2024-05-31 | 江西农业大学 | Composition for preventing and treating bee mites |
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