KR20170058656A - The composition for root-knot nematode control using the fungus Poria cocos. - Google Patents

Abstract

The present invention relates to formulation of a composition for controlling root-knot nematode via a stabilizing method by containing Poria cocos Wolf as an active ingredient. More specifically, the present invention uses a composition for controlling root-knot nematode as an agricultural nematode control agent by formulating the composition. In an attempt to prevent degradation of hyphae of Poria cocos Wolf due to dryness, absorptive starch, corn oil, and specific soil called Dangyu soil are mixed together.

Description

TECHNICAL FIELD The present invention relates to a composition for root-knot nematode control using root-knot nematodes.

The present invention relates to a method for producing a root-knot nematitis control composition made by using a Stabilize formulation.

Recently, the world pesticide market has been gradually decreasing except for Eastern Europe (Agrochemical service, Woodmackenzie, Korea), which is 25.2 billion USD in 1992, USD 30.7 billion in 1998, USD 29.1 billion in 1999, USD 29.2 billion in 2000 and USD 27.94 billion in 2001. 2002). Similarly, the domestic pesticide market has been decreasing to 1.30 trillion won in 2001, 93.0 billion won in 2002, 927 billion won in 2003 and 920 billion won in 2004 (pesticide industry association data, 2005 ) The decrease in the use of these chemical synthetic pesticides in the control of pine reeves, root-knot nematodes and other phytopathogenic fungi has been accompanied by the possibility of adverse effects on the environment and manpower, as well as the rapid changes in consciousness of consumers' This change in eco-friendly, organic cultivation and the government's use of chemical pesticides will continue to curb the use of chemicals in the future. Respectively. As a result, many eco-friendly products derived from natural products with nematocidal and antifungal effects have been developed in the domestic market. However, most of them are sold in the agricultural sector, but most of them are surfactants, nematode fungi, The main ingredient: Azadirachtin or Pyrethrins is used as a raw material, and it is difficult to grasp the exact usage amount because the distribution process and the sales volume are unclear. Also, the effect of reducing leaf root nematode density It is known to be significantly lower than the chemical pesticides used. In general, the root-knot nematocide insecticides using these natural extracts have relatively low toxicity to humans and crops. However, even when the efficacy is low or the efficacy is high in the test tube, It is easy to apply and the insect pests to be applied are different. However, BT, which is a representative example of the microbial preparation, is also used in the field because of the severe environmental change and narrow pest range.

Plant parasitic nematodes, which are distributed widely throughout the world and cause damage to crops, damage the roots of the plant by damaging the plant's surface or by living in soil. Among the various plant parasitic nematodes, rootstock nematodes are the most difficult to control and cause great damage. However, in the case of cultivated control, it is difficult to apply it according to local conditions and cultivated crops, and it takes a high cost, and resistant cultivars are limited to specific crops and are not practical. In the case of chemical agents, the control effect is high, but most of them are toxic when the soil is remained in the organic phosphorus and carbamate system, and damages are increased due to indiscriminate sterilization of useful microorganisms in the soil, Lt; / RTI > As a result, studies on biological control using root-knot nematode microorganisms, biochemical agents and plant extracts have been actively pursued as an alternative to chemical control agents.

In the case of foreign countries, there have been many studies to control root-knot nematodes using fungi for a long time. As a result, several kinds of specific fungi excellent in nematocidal effect have been developed and reported in various literatures. Monacroporium thaumasium, a fungal nematode reported to date, is extremely ineffective in practical plantation and is very difficult to be commercialized for farming. In addition, researches that expected the effects of carnivorous plants using medicinal plants have been carried out, however, it is difficult to supply and receive raw materials, and therefore, there is a drawback in that it is dependent on imports.

The name of Bokryeong originated in China, and it has been used as a tonic and nutritional supplement for a long time. Byeongryeong is a sclerotia that grows parasitic on roots after 3 to 10 years after cutting pine trees. The surface is dark brown, and the inside is grayish-white granular, and smells fresh. The skin is called bokryeopi, and the cells are wrapped with pine roots inside. The white inside color is white roe, and the red inside is red rope. The weakness is mediocre, flavorful and dumb. It has a mild diuretic effect, weak digestive system, excellent effect when there is edema in the whole body, and also effective for nephritis cystitis urethritis. In addition, chronic bruising and bronchial symptoms that are difficult to breathe, such as gut mushroom is a lot of sputum is secreted, and is treated with a combination of gadam and sunburn drugs. In addition, there is the effect of the structure of the stomach, the stomach is excessively stagnant, abdominal bloating and vomiting causes chronic gastroenteritis that causes vomiting, soothing effects and nervous excitement caused by anxiety and anxiety, often surprised, mouth dry, As a stabilizer.

The general components of Byeongryeong were 10.58% ~ 18.04% moisture, 1.19 ~ 2.46% crude protein, 0.95 ~ 1.79% crude fat, 8.12 ~ 9/14% crude fiber and 69.69 ~ 78.02% soluble nitrogen. Free sugars such as glucose, fructose, sucrose, and mannitol were identified. Total free sugar content was 0.187% ~ 0.984%. The content of unsaturated fatty acids was 53.78% ~ 74.54%, which is the main fatty acid of linoleic acid (C18: 2), palmitic acid (C16: 0), stearic acid (C18: 0) and oleic acid . Total amino acid contents were higher in order of tyrosine, aspartic acid, glutamic acid, and serine. The content of pachymic acid and dehydro-pachymic acid in the triterpenes was 168.06mg% ~ 189.47mg% and dehydropachymic acid 34.83mg% ~ 40.71mg%, respectively.

The present invention has been developed in order to solve the problem of the control of root-type nematode of the present invention, and it is confirmed that the bacterium is effective for the insecticide of root-knot nematode, and the composition for controlling root- The purpose is to provide.

In order to achieve the above object, a feature of the present invention is to provide a composition for controlling root-knot nematodes, comprising bacillus subtilis having an effective nematicide as an active ingredient.

In order to prepare a formulation containing the bacterium, a stabilizer for preventing the destruction of the mycelium of the mold due to rapid drying is required, and a composition for controlling the root-knot nematode is formulated using Stabilize formulation with Bacillus subtilis as an effective ingredient .

The stabilize formulation used to formulate the composition for controlling root-knot nematodes is characterized in that it is formulated by mixing ginseng hyphae, water-absorbing starch, corn oil and sugar beet.

The composition for controlling root-knot nematodes is characterized by controlling Meloidogyne incognita (sweet potato root nematode) , Meloidogyne arenaria (peanut root nematode) , and Meloidogyne hapla (carrot root nematode).

The present invention relates to a composition for the control of root-knot nematodes comprising bacillus subtilis as an active ingredient, and is an excellent biological agent capable of replacing existing chemical agricultural chemicals. In addition to the effect of reducing the damage to the pesticide residues in the soil, the use of a culture solution containing a nematode substance produced by an indigenous strain also alleviates damage to root-knot nematodes in the cultivation of environmentally friendly crops. In addition, the bacterium was formulated to prevent the destruction of hyphae, which compensated for the disadvantages of fungal nematode.

 One. Root-knot nematode

The sweet potato root-knot nematode (M. incognita), and peanut root-knot nematode used indoors port black (M. arenaria) carrot root-knot nematode (M. hapla ) were grown in a greenhouse using tomato seedlings.

Tomato seedlings were grown in a plastic pot with a diameter of 16.0 cm and a height of 13.5 cm for 45 to 50 days, and the seedlings were formed for 40 to 45 days.

The roots were cut into 1 cm long roots and the new tomato seedlings were mixed with the seeds in the seedling pots, and tomato seedlings were planted and grown.

2. From the ground eelworm  Separation method

Place a sieve with a size of about 2 mm on the container, pour it into the sieve, wash it thoroughly with water, and pour it into a sieve with two sieves of 175 μl and 50 μl in size.

This method was repeated 3 times or more to collect 50 μl of the supernatant in 175 μl sieve. The collected supernatant was washed with water and collected in a beaker, and then placed in a nematode separator funnel for about 24 hours. .

 3. Bombyx mori Poria  cocos Wolf ) Mycelium eelworm  Control Effectiveness

Poria cocos Wolf was cultivated in PDA (Potato Dextrose Agar) medium, and nematodes were divided into 30, 4, 12 and 24 hours.

At this time, even when physical force is applied to the nematode, it is considered dead when there is no movement.

Inoculation time (h) M. incognita
(Sweet potato root nematode) M. arenaria
(Peanut rootworm nematode) M. pills
(Carrot root nematode) 0.5 14.5 12.4 13.8 4 70.5 67.8 65.5 12 93.6 93.0 92.8 24 94.0 93.0 93.0

In Table 1, when insecticidal effect on nematode mycelium was examined, when 3 kinds of root nematode species were placed on Bombyx muralis, the insecticidal effect was more than 65% after 4 hours, and 93% And it was effective for the control of root - knot nematodes.

However, Poria  cocos Wolf ) Formulation

PDB (Potato Dextrose Broth) medium was inoculated with Bacillus subtilis spores and incubated at 28 ° C and 120 rpm for 7 days with shaking. After that, the bacillus subtilis was solidified in order to preserve it as a storable form until use.

In order to make a formulation containing fungal hyphae, stabilizers are required to prevent the destruction of mold mycelium due to rapid drying, and the most commonly used stabilizers are pregelatinized starch and oil.

ingredient content(%) stabilizer The pregelatinized starch 18.5 corn oil 18.5 kaolin 37 water 18.5 Active ingredient Hyphae hyphae 7.5 100

A stabilizer is added to the formulation to form a bacillus subtilis.

Mycelia of mycelia were vacuum filtered with mycelia cultured in PDB (Potato Dextrose Broth) medium for 7 days.

1) Mix 20 g of mycelia of mold with 50 ml of water and 50 ml of cooking oil, add 50 g of modified starch, and mix with a blender for 30 seconds.

2) Leave the dough at room temperature for 1 hour.

3) Add 100 g Kaolin paste to the dough and mix well. Then, mix noodle machine 5 times and mix well.

4) Mix the thinly spread dough in aluminum foil and dry in a clean bench overnight.

5) I use a blender to dry the dried preparation overnight.

6) A finely divided preparation was sieved using a 20 mesh sieve, and a uniform size formulation was used for the test.

7) For the long-term storage test, the modified starch preparation is placed in a plastic bag and stored at 5 ° C in a refrigerator and 25 ° C at room temperature.

5. Benthos  Solid Pot  black

Benthic organisms (3%, 5%) and nematode infected soil were placed in a plastic bag and mixed well.

After putting the soil in the soil, I put water in the greenhouse for 10 days by covering the newspaper with water so that the water did not flow.

After 10 days, three or four tomato seedlings, which had been previously cultivated in a plug port containing sterilized sand, were transplanted with one drop per pot.

As a control, nematode infected soil was treated with no treatment.

The degree of propagation of nematodes by each treatment was examined by examining the number of shoots of root - knot nematode in roots after 60 days of tomato implantation.

The ovariectomy was done by carefully washing the tomato roots carefully in water, immersed in a 0.15% Phloxin B solution, stained for 15 minutes, and the number of reddish-stained oocysts was visually examined in a container with a white bottom.

inoculation M. incognita
(Sweet potato root nematode) M. arenaria
(Peanut rootworm nematode) M. pills
(Carrot root nematode) control 288 a 307 a 295 a 3% 158 b 166 b 162 b 5% 143 b 148 b 152 b

Table 3 shows the inhibitory effects of root-knot nematode on benthic preparations.

In the 3% and 5% treatments of the tomato transplantation, the inhibition of nematode densities was observed in the soil treated with 3%, 5%, and 25% of the rootstock nodules, 46% of the peanut rootstock nematodes, The root - knot nematode showed a high inhibition rate by inhibiting the nematode density by about 45%.

When 5% treatment of bamboo sprout preparation was applied, the inhibitory effect of nematode was shown to be highly significant because it showed a density inhibitory effect of about 50% or more per nematode. (p = 0.01)

Claims (5)

A composition for preventing root blight nematodes containing Poria cocos Wolf as an active ingredient.         [3] The composition according to claim 1, wherein the hyphae mycelia used in the composition for controlling root-knot nematodes are used by vacuum filtration of liquid cultured hyphae.         [3] The composition according to claim 1, wherein the composition for controlling root blight nematode is made into a Stabilize formulation by using Mycelia sp.         The composition according to claim 3, wherein the Stabilize formulation is prepared by mixing water-absorbing starch, corn oil, and sugar beet by using bacillary mycelium. In each claim, the root-knot nematode is characterized by Meloidogyne incognita ( Melonogyne incognita ) , Meloidogyne arenaria (Peanut root nematode) , Meloidogyne hapla (Carrot root nematode).