KR101407191B1 - Composition for controlling the diseases of red pepper plants and control method using it - Google Patents

Abstract

The present invention relates to a novel use of insecticidal active substances isolated from insect pathogenic bacteria, and more particularly to a composition for controlling red pepper disease and a method for controlling red pepper disease using the same. The present invention confirms the new efficacy of indole, which is known as the insoluble metabolite of Xenopus nematophila K1, and provides a new use of indole as an antiseptic for pepper blight or pepper anthracnose based on these results. The composition for controlling a red pepper disease of the present invention contains indole as an effective ingredient for controlling pepper blight or pepper anthracnose. In addition, the present invention provides a method for controlling red pepper disease, which comprises treating indole at the above ground of red pepper or at the above-ground rhizosphere soil or pepper to control pepper blight or red pepper anthracnose. The present invention can be widely used as an effective method for preventing and treating pepper blight or pepper anthracnose, and can be widely used as an alternative to conventional chemical pesticides which have many problems such as low effectiveness and resistance generation.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for preventing red pepper disease, and a method for controlling red pepper plants and control method using the same.

The present invention relates to a novel use of insecticidal active substances isolated from insect pathogenic bacteria, and more particularly to a composition for controlling red pepper disease and a method for controlling red pepper disease using the same.

Although more than 10 kinds of diseases occur in Korean red papermaking area every year (Seo et al., 2011), it is known that pests and anthrax have the greatest damage. Phytophthora capsici greatly reduces the yield of pepper cultivation. Once it is developed, it is almost impossible to treat and it is now known to be the best way to prevent disease development. Cultivation of cultivars, cultivation of crops, and crop rotation have been carried out for the control of plague, but they have not been effective. Pepper anthracnose is a major obstacle to the production of red pepper in addition to pepper blight, causing leaf, stem and fruit disease, and the damage caused by fruit anthrax disease mainly occurs in mature fruit is more than 95%. Pepper anthracnose is known to be an effective way to remove diseased parts early in the process and to remove all traces of diseased plants after harvesting to reduce the number of infectious agents in the following year.

More than 70 species and 40 kinds of plant protection products have been notified to Korea crop protection association (2011) for anthrax and pestilence, but the control effect is not great. There are also few registered biopesticides for pepper anthracnose and plague (Industrial evaluation agency for Gyeongbuk province, 2007). The most effective control method for these diseases is chemical control using an organic synthesized bactericide. However, these chemical controls have caused many problems such as resistance to chemical pesticides, residual toxicity, acidification of soil, toxicity of humanoids, effects on natural enemies, contamination of drinking water sources and destruction of ecosystems, The development of low toxicity and environmentally friendly pesticides that can be applied to diseases is urgently required.

Xenorhabdus nematophila K1 is an insect pathogenic bacterium isolated from the insect parasitic Steinernema carpocapsae (Park et al., 1999). The antimicrobial activity of benzylidene acetone, a metabolite produced by this bacterium, against plant pathogens has been reported (Yi et al., 2007). Thereafter, another substance indole produced by X. nematophila K1 was isolated and its insecticidal effect was known (Seo, 2012). Indole compounds have been reported to be metabolites of plants or bacteria in their natural state (Kim et al., 1991; Pedras and Hosain, 2011), antimicrobial activity against human pathogens (Sung, 2008; Lee and Wood, 2009) The antimicrobial activity of the compounds against plant pathogens and the control of plant diseases using indole compounds have also been reported (Kim et al., 2005; Kim et al., 2007; Pedras and Hosain, 2011).

Korean Patent Registration No. 10-0946633 discloses indole as one of metabolites derived from microorganisms together with 3-acetyl-1-propanol, 3-methyl-1-butanol, and the like. Promoting and controlling plant diseases.

However, neither in the prior art nor in the patent literature discloses or suggests that indole has a direct control effect on red pepper pluck or red pepper anthracnose.

Korean Patent Registration No. 10-0946633

Industrial evaluation agency for Gyeongbuk province. 2007. Industrial road-map for bio-health in Kyeungbuk province (revised.). Daegu, Korea. 324pp. Kang, Y. and Chung, Y. 2003. Chemical screening for control of metalaxyl-resistant tobacco black shank disease. Res.PlantDis. 9: 224-228. Kim, D., Kim, J., Shim, J. and Hwang, I. 2005. Synthesize indolederivates for control of tomato bacterial wilt. Program and abstract. 2005 Fall meeting of the Korean society of pesticide science. p.43. (abstract). Kim, H., Hong, K., Choi, G., Jang, K. and Rhyu, C. 2007. Control effect of indolebutylic acid (IBA) on rice blast. Kor. J. Pesti. Sci. 11: 313-319. Kim, J., Lee, E., Park, S. and Sin, G. 1991. Indole contents and microorganisms producing indole of traditional Korean soybean paste. Environ. Research 11: 31-36. Korea crop protection association. 2011. Aguide to agrochemical usage. Seoul, Korea. 1,311pp. Lee, J. and Wood, T. 2009. Roles of indole compounds in pathogenic biofilm and virulence. 2009 spring meeting and international symposium. Kor. society for biotechnol. bioengineering. P.81. (abstract). Park, S., Jun, M., Chun, W., Seo, J., Yi, Y. and Kim, Y. 2010. Control effects of benzylidenacetone isolated from Xenorhabdus nematophila K1 on the diseases of red pepper plants. Res. PlantDis. 16: 170-175. Park, Y., Kim, Y. and Yi, Y. 1999. Identification and characterization of a symbiotic bacterium associated with Steinernema carpocapsae in Korea. J. Asia-Pacific Entomol. 2: 105-111. Pedras, S. and Hosain, S. 2011. Interaction of cruciferous phytoanticipants with plant fungal pathogens: indole glucosinolates are not metabolized but the correponding desulfo-derivatives and nitriles are. J. Phytochemistry 72: 2308-2316. Seo, J., Yi, Y., Kim, B., Hwang, J. and Choi, S. 2011. Disease Occurrence on Red-pepper Plants Surveyed in northern Kyeungbuk province, 2007-2008. Res. Plant Diss. 17: 205-210. Seo, S. 2012. Chemical identification and biological characterization of Phospholipase A2 inhibitors synthesized by entomopathogenic bacteria, Xenorhabdus nematophila and Photorhabdus temperata subsp. temperate M.S.Thesis. Andong National Univ., Andong. (Inpress). Sung, O. 2008. Antimicrobial effects and the mode of indole-3-carbinol against human pathogenic microorganisms. M.S. Thesis. Dept. microbiol. Kyungpook National Unive. 59 pp. Y., Park, H. W., Sony, S., Seo., J., Kim, Y. O., Shin, C. S. and Kim, Y. 2007. Identification of two entomopathogenic bacteria from nematode pathogenic to the oriental beetle, Blitopertha orientalis. J. Microbiol. and Biotech. 17: 968-978.

The present invention is to identify the novel efficacy of indole, known as the insoluble metabolite of Xanthrobacteriumatophila K1, and to provide its use accordingly. In particular, it has been confirmed that indole has an anti- The present invention provides a composition for controlling a disease caused by a red pepper and a method for controlling a red pepper disease.

In the present invention, it was confirmed that the component isolated from the culture broth of Xenoprobus nematophilus K1 was indole, and the antibacterial activity of indole against pepper spp. And anthrax was examined. As a result, Of the mycelial growth. These antimicrobial activities were maintained for more than 60 days under natural sunlight conditions and were not lost in soil water. In addition, the inhibitory effect of indole on P. aeruginosa and anthracnose bacterium was confirmed by the port experiment. Also, in the field control experiment conducted in the actual pepper field, the indole suspension was sprayed on the soil before the pepper was settled in the field, Spraying on the ground resulted in a 30% reduction in major outbreaks.

Based on the above-described experimental results, in the present invention,

There is provided a composition for controlling red pepper disease, comprising indole as an active ingredient for controlling pepper blight or pepper anthracnose. Preferably, the composition comprises an effective amount of indole at an effective amount of 50 to 1,000 占 퐂 / ml when used as a preservative. In addition to the indole content, It also includes cases where dilution is used in the range. In addition, the composition of the present invention may further comprise an agricultural electrodeposition agent. At this time, the electrodeposition agent may be included in the composition of the present invention within a range of known doses. In addition, the composition of the present invention may further contain conventional additives that can be used in agricultural chemicals.

In addition, based on the above-described experimental results,

There is provided a method of controlling red pepper disease comprising treating red pepper with indole at the top of the rhizosphere soil or red pepper in which the red pepper is to be formulated or formulated to control the red pepper pluck or red pepper anthracnose. The term "treatment" in the present invention is meant to include all treatment methods of known controlling agents such as crossing, spraying and mixing, unless otherwise specified. The term "top part of pepper" is meant to include all parts of the ground including the leaf, stem, and fruit of pepper.

The method of controlling red pepper disease provided by the present invention preferably includes treating the composition containing indole at a concentration of 10 to 100 占 퐂 / ml in the rhizosphere soil in which the red pepper is to be formulated or formulated. In this case, the treatment includes, in particular, mixing treatment with a method such as spraying the composition on the untreated soil, and crossing and spraying before and after the preparation. In addition, the method of controlling the red pepper disease of the present invention preferably includes spraying a composition containing indole at a concentration of 100 to 1,000 占 퐂 / ml on the ground part of a formal red pepper, more preferably, Spray the composition containing indole at a concentration of 200 to 500 [mu] g / ml. In a preferred embodiment of the method for controlling red pepper disease of the present invention, a composition containing indole at a concentration of 50 to 100 占 퐂 / ml is applied to the rhizosphere soil to which the red pepper is to be formed, and after the red pepper is formulated, The composition containing indole is sprayed at a concentration of 200 to 500 占 퐂 / ml at intervals of one day. More preferably, the composition containing indole at a concentration of 80 to 100 占 퐂 / ml is applied to the rhizosphere soil to which the pepper is to be planted. After the red pepper is planted, 200-300 占 퐂 / Ml < / RTI > Further, preferably, the composition containing the indole may further comprise an agricultural electrodeposition agent.

In the present invention, it is confirmed that indole, which is known as a new insecticidal metabolite of Genovese nematophila K1, has a controlling effect against red pepper pox and anthracnose and provides indole as an antiseptic for pepper blight and anthrax. Until now, pepper plague and anthrax were the most damaging of the red pepper disease, but it was difficult to control. The present invention can provide a new controlling agent and a controlling method which can be applied in actual farming field by confirming the control effect of indole, which is an insecticidal substance which is identified and isolated from an insect hospital bacterium, against the red pepper pests and anthrax. In particular, matalaxyl wetting agent, which is a penetrating fungicide used to control tobacco leaf blight which is a current infectious disease, can not apply the rhizome method to rhizospheric soil due to the occurrence of weakness in pepper, Can be used more effectively than metalaxyl hydrate, which is dependent only on foliar spraying, because it can control the blight without causing harmful effects in the treatment of indole 100 占 퐂 / ml. In addition, since the antimicrobial activity of the indole can be maintained for over 60 days under the natural sunlight condition, the controlling composition of the present invention can maintain the high controlling effect without frequent spraying.

Fig. 1 shows the results of the experiment for controlling the effect of the medium containing indole, wherein a is pepper reverse bacillus, b is anthracnose, c is pepper foot rot, d is rot fungus, e is bacterial fungi, and f is ulcer Respectively. The results are shown in Fig.
Fig. 2 shows the results of an experiment on the control effect of pepper root rot and anthrax bacterium after preserving indole-containing medium under natural sunlight. 2 and 3 show results of inoculation and cultivation of pepper seed bacterium after preserving the medium under natural sunlight for 15 days and 60 days, respectively, and c and d show the result of culturing the medium under the natural sunlight for 15 days and 60 days And after inoculation with pepper anthracnose.
FIG. 3 shows the results of an experiment on the control effect against P. aeruginosa in a medium containing indole suspension at various concentrations passed through field soil.
Fig. 4 relates to the inhibitory effect of indole. In Fig. 4, a and b show the indole suspension of 200 / / ㎖ and 250 / / ㎖, respectively, and the indole suspension of 1,000 ㎍ / It is a case of spraying on leaves and stems.
FIG. 5 is a photograph of an untreated control group in which indole is not treated in the soil, and b is a photograph showing the effect of mixing the indole suspension in the soil and the red pepper seedlings, This is a photograph of the test group.
6 is an experimental result of spraying indole suspension of 100 / / ㎖ to 500 / / ㎖ on the red pepper fruit inoculated with anthrax anthracis and observing the degree of lesion formation of the red pepper fruit.
FIG. 7 is a graph showing the results of experiments in which indole and benzylidenacetone (BZA) were sprayed onto the soil before the capsicum preparation and the red pepper was transplanted into the soil, As a result, "a" is a red pepper blight, "b" is anthrax disease, "c" is a cloudiness disease, and "d" is a black fungus disease prevention experiment result.
Figure 8 shows the dry weight of the red pepper fruit harvested in the test group in which the indole suspension was sprayed compared to the control and BZA treated groups.

Hereinafter, the present invention will be described in more detail with reference to specific examples. However, these embodiments are only for describing the present invention more specifically, and the scope of the present invention is not limited by these embodiments.

[Materials and Methods]

Pepper varieties, pathogens and reagents used

Chili pepper was used for sowing and seeding commercially available seeds (breed: King). Indole (Aldrich, USA) was dissolved in dimethyl sulfoxide (DMSO, Hayashi Pure Chemical Ind.) To make 100,000 / / ml solution and diluted with distilled water. In the case of Phytophthora capsici (KACC 40483), Colletotrichum acutatum (KACC43125), Ralstonia solanacearum (KACC11171), Pectobacterium carotovorum subsp. Carotovorum (KACC11131), Xanthomonas campestris pv. Vesicatoria , KACC11158), and ulcer disease ( Clavibacter michiganensis subsp. Michiganensis , ATCC 20883) were purchased from the Rural Development Administration's Agricultural Genetic Resources Information Center and cultivated at 28 ℃ for one week in potato agar plate medium (PDA, Difco, USA).

Identification of indole

X. nematophila K1 (KACC 91136) was extracted with ethyl acetate (EtOAc) and concentrated. The residue was subjected to primary purification by column chromatography and then secondary purification by thin layer chromatography to give Rf = 0.47 (EtOAc: hexane = 9: 1). The structure of the unknown compound was identified by NMR and elemental analysis, and it was identified as indole. As a result of the comparison with the reagent indole purchased from Aldrich, it was confirmed that it was the same material.

_{^{1 HNMR (400MHz, CDCl 3)}} δ = 8.0 (br s, 1H), 7.6 (d, J = 8.0 Hz, 1H), 7.3 (dd, J = 8.0, 0.8Hz, 1H), 7.2-7.1 (m, 2H), 7.05 (td, J = 8.0, 0.8 Hz, 1H), 6.50-6.47 (m, 1H); _{^{13 CNMR (400MHz, CDCl 3)}} δ = 135.9, 127.9, 124.3, 122.1, 120.9, 119.9, 111.2, 102.7; Elemental Analysis: calcd C 82.02%, H 6.02%, N 11.96%; found C 82.01%, H 6.27%, N 11.72%.

Investigation of antibacterial activity

Colonies of Pepper spp. Or anthrax bacteria having a diameter of 6 mm in diameter were inoculated to the center of the potato agar plate medium to which 250 / / ml and 500 / / ml of indole were respectively added and cultured at 28 캜 for one week. The colony diameter of the cultured hospital fungus was compared to the colony diameter of the hospital fungus grown in medium without indole. Three petri dishes were used for each strain. In addition, a suspension (10 ⁹ cfu / ml) of pepper foot rot fungus, brucellosis fungus, bacterial fungus bacillus, and ulcer disease was applied to a nutrient agar plate medium (Difco, USA) and BZA · indole · oxindole A filter paper (diameter 6 mm) having absorbed the drug suspension (1,000 占 퐂 / ml) was placed thereon. These bacteria were incubated at 30 ℃ for two days and the bacterial inhibitors formed were investigated. Three petri dishes were used for each strain.

Investigation of duration of antibacterial activity

The potato agar plate medium containing 250 / / ml of indole was stored for 15 days and 60 days under natural sunlight, respectively. Colonies of 6 ㎜ diameter pepper sprout or anthrax were then inoculated to the center of the plate, For one week. The colony diameter of the cultured hospital fungus was compared to the colony diameter of the hospital fungus grown in medium without indole. Three strains of 3-Petri dish were used for each strain.

Investigation of adsorption on soil particles

A suspension of 250-1,000 占 퐂 / ml of indole was soaked in the beaker containing the soil of the field so that the soil was soaked and stored for one day. The next day, the indol suspension was collected, centrifuged at 2,000 rpm, and the supernatant was collected and passed through a bacterial filter. A PDA (Difco) was added to the indole suspension thus passed through the soil to prepare a potato agar plate culture medium, and a colony of Pseudomonas aeruginosa having a diameter of 6 mm was inoculated at the center thereof and cultured at 28 ° C for 7 days. The colony diameter of the cultured capsicum bacterium was measured and compared with the diameter of the inverted bacteria colonies cultured in the untreated potato agar medium. Three treatments were performed for each treatment.

Weak investigation

50 μl of indole 200 μg / ml or 250 μg / ml suspension was pipetted into the rhizospheric soil of the red pepper planted in a plastic pot (diameter 25 cm, height 12.5 cm). In addition, a suspension of 250-1,000 占 퐂 / ml of indole was sprayed on the leaf and stem of the red pepper planted in a connection pot of 6 占 centimeter, and the occurrence of the weakening was observed for one week.

Investigation of disease control effect

1. A horticultural soil sterilized in plastic bag (60 × 30 × 15 cm) and 300 ml of indole suspension (100 μg / ml) were mixed and transferred into a 6-staged porridge of about 30 ㎝ in length. The suspension of Pepper spp. Was distributed in the rhizosphere soil at 45 ㎖. The sterilized suspension was prepared by adding sterilized water to the mixer and diluting it to 90 ㎖ by adding the sterilized germs cultivated in the potato agar medium (diameter 9 ㎝) for one week. After 6 weeks of treatment, the mice were inoculated with 3 replicates. In addition, the surface of the sufficiently grown red pepper was scratched with an insect pin and then sprayed with a suspension of pepper anthracis (10 ⁸ spores / ㎖). After one hour, 100 μg / ml of indole, 200 μg / ml, 250 μg / ml and 500 μg / ml of suspension were respectively sprayed. And 6 repeats of pepper fruits at the concentration of indole.

2. Inoculated with red lentil suspension (100 ㎍ / ㎖) in 20 liters per 60 drops before the red pepper was planted in the red pepper field at Chungwolli in Andong city, Gyeongsangbuk province. After the red pepper was formulated, 250 μg / ml of indole and 500 μg / ml of suspension were sprayed on leaves and stems at intervals of 10 days. To the indole suspension, 0.05% of polyoxyethylene alkyl aryl ether (10%, sodiumligno sulfonate 20%). The main disease outbreaks were compared with the control group treated with benzylidenacetone (Aldrich 241091) and the untreated control group. Pepper blight was the predominant onset rate (%), pepper anthracnose and blight were the onset rate (%), and bacterial blotch fungus was onset rate (%). The treatment was repeated 60 times per treatment. The dried weight was examined by harvesting the red pepper fruits grown in 12 treatments. The red pepper, which was not found to be commercially viable, was excluded from the survey.

[result]

Antimicrobial activity against pepper spp. And anthracnose

Both the pepper spp. And anthrax spp. Did not grow at all in medium containing 250 / / ml as well as 500 / / ml of indole (Fig. 1 a, b). However, no growth inhibition source was formed in the vicinity of the filter paper impregnated with the drug suspension of 1,000 μg / ml, as in the case of sterilized distilled water, for the pepper foot rot fungi, the rot fungi, the bacterial fungi and the ulcer bacilli (c, d, e , f). In a previous study, Sung (2008) investigated the antibacterial activity of indole-3-carbinol (I3C) on human pathogenic bacteria and found that the antimicrobial activity against Gram-positive bacteria was stronger than that of Gram- Respectively. Sung explained that gram-negative bacteria are resistant to I3C because LPS in the cell wall membrane inhibits the antibacterial activity of indole compounds. However, in the experiment of the present invention, the inhibitory effect was not shown in the gram-negative bacterium as well as the Gram-positive bacterium of pepper ulcer, even at the treatment of 1,000 ㎍ / ml of indole. Therefore, it was concluded that indole could be used as a pesticide for control of bacterial diseases of pepper though it is possible as a disinfectant for control of red pepper or anthrax.

Duration of antimicrobial activity in natural conditions

Even when the potato agar plate medium containing 250 / / ml of indole was stored for 15 days under the natural sunlight and inoculated, neither pepper nor anthracnose bloomed at all (Figs. 2 (a) and 2 (c)). In addition, even when the medium was inoculated on a medium preserved for 60 days under the sunlight, the growth of microorganisms and anthracnose was extremely poor (Fig. 2b, d). Therefore, it was concluded that the inhibitory effect of indole suspension (250 ㎍ / ㎖) sprayed on the leaves and stems of red pepper against rotavirus and anthracnose would persist for more than two months unless washed away.

Antimicrobial activity in soil water

As a result of cultivating pepper seed germs in a potato agar medium prepared from an indole suspension passed through a field soil, the medium prepared with the indole suspension passed through the soil did not grow any germs at all the indole concentrations exceeding 250 / / ㎖ ). Therefore, it is considered that the indole is not adsorbed or inactivated in the soil particles and can maintain the activity in the soil water to inhibit the growth of bacteria.

Weakly occurring concentration

The red pepper cultivated with the indole suspension of 250 / / ml in the rhizosphere soil began to dry at the edges of the leaves and finally showed a decadent mild symptom (Fig. 4b). However, no symptom of weakness was observed in the red pepper cultivated with the indole suspension of 200 / / ml (Fig. 4 (a)). In addition, when the indole suspension was sprayed on leaves and stems, no symptoms of weakness were observed even when sprayed at a concentration of 1,000 μg / ml (FIG. 4C). It is not clear why the incidence of weakness at relatively low concentrations when the indole suspension is applied to the rhizosphere soil of red pepper compared to the red pepper leaves and stems. According to the phytotoxicity test method for the notification of plant protection products, when used at a concentration of 2 times the recommended concentration for the use of plant protection products, the plant should not cause any harm. Therefore, the concentration of indole suspension was less than 100 ㎍ / ㎖ in the case of roots in the rhizosphere of the pepper and less than 500 ㎍ / ㎖ in the case of spraying on the leaves and stems of the pepper.

Disease control effect

After mixing indole suspension (100 ㎍ / ㎖) into soil, the red pepper seedlings were transplanted and the inoculated suspension was inoculated. As a result, the untreated control group, which did not mix the indole suspension with the soil, Approximately 30% of the red peppers in the test group transplanted into the soil treated with indole infected with the plague (Fig. 5). Therefore, it was concluded that 100 ㎍ / ㎖ of indole suspension could be used to control the disease. In addition, 100% anthracnose lesion was formed in the pepper of the untreated control group in which the indole suspension was not sprayed, but the test was performed by spraying 100 to 250 / / ml of the indole suspension The lesion formation was about 25% in the red pepper fruits of the group, and about 10% in the red pepper fruits of the test group sprayed with the 500 μg / ml indole suspension (FIG. 6). In the previous phytotoxicity test, since no mild symptoms were observed in the pepper leaves sprayed with 1,000 mg / ml of indole suspension (FIG. 4), an indol suspension of less than 500 μg / ml was sprayed to obtain a good anthrax control effect .

2. An indole suspension was sprayed from the red pepper field in Andong City, Gyeongsangbuk-do to the top of the red pepper before planting and the major pathogenesis was investigated (Fig. 7). As a result, in the red pepper sprayed with 250 / / ml of indole on the surface of the pepper, the percentage of onset plague disease was about 35% (Fig. 7a), the anthracnose onset was 35% The foliage infestation rate (d in Fig. 7) and the onset incidence of mold disease were reduced by about 40% (Fig. 7C).

This disease control effect was the same as the disease control effect of red pepper treated with BZA (benzylidenacetone) at the same concentration. In the pepper sprayed with 500 ㎍ / ㎖ of indole suspension, black fungus infection rate was about 10%, which was superior to 250 ㎍ / ㎖ of indole. However, the control effects of the other three diseases were the same at 250 ㎍ / ㎖ and 500 ㎍ / ㎖ of indole. Peppermilk is known to penetrate into the fruit through holes drilled into cigarette moth, pepper fruit, and no other pathway has been reported for pathogenic bacteria to enter the fruit. Therefore, although the inhibitory effect on the growth of pepper - millet was not observed, it was thought that the decrease in onset rate due to rotting in the pepper sprayed with indole may be attributable to the insecticidal effect of indole on tobacco moth. The dry weight of the fruit harvested from the red pepper sprayed with the indole suspension was increased by about 30% as compared with the untreated red pepper (FIG. 8), and the effect of this growth was thought to be due to the disease control effect. However, no difference was found between the concentration of indole and the difference between the treated and the treated red pepper treated with BZA suspension.

As a most preferable example, 100 μg / ml suspension of indole is sprayed on the soil and the red pepper is formulated. Then, 250 μg / ml suspension of indole is sprayed on the ground portion of the pepper It is considered that about 30% of plague, anthracnose, milky and black mold diseases can be controlled by spraying. However, the amount of indole used, treatment method, interval, etc. can be adjusted depending on the situation. Tobacco plague and red pepper plague are all infectious diseases. Currently, matalaxyl wetting agent, which is a penetrating fungicide, is used to control tobacco rhizome (Kang and Chung, 2003). However, due to the occurrence of weakness in pepper, it can not use the metalaxyl hydroponics method for rhizospheric soil, and it relies on the protection of the ground by chemical foliar treatment (Korea crop protection association, 2007). However, according to the present invention, the indole can be effectively used for the control of the main disease of pepper than the metalaxyl hydrating agent which is dependent only on the foliar spraying, since the pest can be controlled without generating the harmful effect in the pepper even in the soil treated with the indole 100 μg / ml suspension . In addition, since the antimicrobial activity of indole can be maintained for more than 60 days under natural sunlight conditions, if the rain does not come frequently, the interval of drug application can be extended longer than 10 days applied in the experiment. In addition, it is also possible to reduce the spraying interval if necessary by increasing the effect or frequent rain.

INDUSTRIAL APPLICABILITY The composition and method of controlling the red pepper disease of the present invention comprising indole as an active ingredient can be widely used as an effective method for preventing and treating red pepper blight or red pepper anthrax, And can be widely used as a substitute for chemical pesticides.

Claims (10)

A composition for the prevention of red pepper pox or red pepper anthrax comprising indole as an active ingredient for controlling pepper blight or pepper anthracnose. The composition according to claim 1, wherein the composition contains an effective amount of indole of 50 to 1,000 占 퐂 / ml when used as a control agent. The composition according to claim 1 or 2, further comprising an agricultural electrodeposition agent for controlling anthrax or pepper anthrax. A method for the control of red pepper disease, which comprises treating the indole at the above ground of red pepper or at the top of the red pepper. 5. The method for controlling red pepper disease according to claim 4, wherein the red pepper is treated with a composition containing indole at a concentration of 10 to 100 占 퐂 / ml in a rhizosphere soil to which the red pepper is to be planted. 5. The method for controlling red pepper disease according to claim 4, wherein a composition containing indole at a concentration of 100 to 1,000 占 퐂 / ml is sprayed on the ground part of the red pepper. The method for controlling red pepper disease according to claim 6, wherein a composition containing indole at a concentration of 200 to 500 占 퐂 / ml is sprayed on the ground part of the red pepper. 5. The method of claim 4, wherein the composition containing indole at a concentration of 50 to 100 占 퐂 / ml is applied to the rhizosphere soil to which the pepper is to be planted, Lt; RTI ID = 0.0 > pg / ml < / RTI > 9. The method according to claim 8, wherein the composition containing indole at a concentration of 80 to 100 占 퐂 / ml is applied to the rhizosphere soil to which the pepper is to be planted and the pepper is formulated. Lt; RTI ID = 0.0 > pg / ml < / RTI > 10. The method of controlling a red pepper disease according to any one of claims 5 to 9, wherein the composition further comprises an agricultural electrodeposition agent.

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