KR20090066412A - Methods for plant growth promotion and plant protection by bacterial metabolites - Google Patents

Abstract

A composition for promoting plant growth and preventing plant diseases, which contains microorganism derived metabolite is provided to induce the resistance against plant diseases, promote the growth of plant and protect the plant from the insect. A composition for promoting plant growth and preventing from plant diseases comprises microorganism derived volatile metabolite as an active ingredient. The microorganism comprises Bacillus sp. and Paenibacillus sp. The microorganism is Penibacillus polymyxa](KCTC 8801P). The volatile metabolite comprises 3-acetyl-1-propanol, 3-methyl-1-butanol, indole, isoamyl acetate, and butyl acetate. The plant diseases are bacterial soft rot, wild fire, anthrax, phytophthora blight, damping-off, fusarium wilt, ceitocybe bescens, grey mould, bacterial wilt, leaf blight, and fusarium wilt.

Description

Method for plant growth promotion and plant protection by bacterial metabolites

The present invention relates to a composition for promoting plant growth and controlling plant diseases comprising a volatile metabolite derived from microorganisms as an active ingredient, a method for promoting plant growth using the same, and a method for controlling plant diseases.

As the social demand for the development of environmentally friendly bio-derived agricultural materials for the development of sustainable eco-friendly agriculture and the safe production of agricultural products has been increased, researches for the development of materials secured from the natural resources of agricultural organisms have been steadily pursued. . One of them is to search for and utilize microorganisms beneficial to plants from the natural world, and Paenibacillus bacteria have been studied as a part. Some of the mechanisms that help bacteria in the genus Pannibacillus have been reported include nitrogen fixation, solubilization of insoluble phosphoric acid, production of plant growth hormones (auxin, cytokinin, etc.), and production of antibiotics. It has been reported to promote and promote disease resistance. However, one of the frequently encountered problems in using agricultural microorganisms so far developed is that field efficacy in actual packaging is less than or expected to be lower than expected. This may be inevitable because the living organisms are to be administered to a diverse and time-varying environment, but the development of techniques to overcome them is important for increasing the utilization of eco-friendly agricultural microorganisms or agricultural materials.

The present inventors have isolated the Panic barylus polymyx E681 bacteria from the roots of winter barley and found that they have excellent usefulness for crop growth, soil disease control, etc. (Ryu CM et al. , 2005. Journal of Microbiology and Biotechnology 15 : 984-991; Ryu CM et al., 2006. Biological Control 39 : 282-289) A Korean patent has been secured for the strain (Registration No. 0020402, Deposited Strain No .: KCTC 8801P). At the time, it was named Bacillus polymyxa E681, but it was then panned by a newly introduced classification system by Ash C. et al. (Ash, C. et al. , 1993. Antonie van Leeuwenhoek. 64: 253-260) . By applying the genus name of Baen ( Paenibacillus ) it was renamed to the current strain name 'Panibacillus polymyxa E681'. E681 is known to have the ability to promote the growth of various crops, such as cucumbers, sesame seeds, peppers, tomatoes, barley (Patent No. 0220402). Recently, the inventors of the present invention revealed the entire genome sequence of E681 bacteria and analyzed the gene function to produce antibiotics such as polymyxin and fuzacidin, cellulose, chitin, xylan, pectin, phosphatase and other degradation enzymes. By revealing that it contains a number of genes involved in enzyme production and interaction with plants, genetic information is available to support the useful properties of this strain in relation to plants (Jeong H. et al ., 2006. J. Microbiol Biotechnol . 16 : 1650-1655; Choi S.-K. et al. 2007. Biochem. And Biophys.Res.Commun . 365 : 89-95). In another study, 2,3-butanediol, a volatile metabolite produced by Bacillus subtilis GB03 and Bacillus amyloliquefaciens IN937a, promotes growth of Arabidopsis and It has been shown to enhance induced systemic resistance to Erwinia carotovora subsp.carotovora (Ryu CM et al. , 2003. Proceedings of National Academy of Sciences in USA 100 : 4927-4932 Ryu CM et al ., 2004. Plant Physiology 134 : 1017-1026; Farag, MA et al ., 2006. Phytochemistry 67 : 2262-2268). Subsequent studies revealed that GB03 and IN937a strains produced about 30 kinds of volatile metabolites, but there was a difference in the type of each product. These small molecule substances influenced the growth of crops by strain-specific mechanisms. (Farag MA et al ., 2006. Phytochemistry 67 : 2262-2268).

In the rhizosphere and soil, various microorganisms coexist in complex interrelationships, and the types of microorganisms living in relation to plants may vary according to the type of plant and the soil environment. In terms of microorganisms, the target plants may be affected by the materials and other properties they produce. Therefore, it is necessary to secure as many kinds of microorganisms as possible for agricultural use, and it is important to obtain various metabolites representing plant useful functions and to clarify their function in detail. Research on plants useful bacteria so far, a lot of research on the mainly Gram-negative bacteria Bra Grundig Division Away (Bradyrhizobium) inside, rayijo Away (Rhizobium) genus Pseudomonas (Pseudomonas) in the azo RY rilreom (Azospirillum) in bacteria, such as done There is relatively little research on Gram-positive bacteria and more research and development is needed.

The research to search for plant-derived substances in the metabolites of microorganisms has been conducted for a long time, but there are not many examples of practical use in agriculture. However, technologies that can control the growth of plants in small amounts or improve resistance to diseases or environmental stress, rather than organic synthetic fertilizers of conventional concepts in crop production systems, will play an important role in the development of eco-friendly agriculture in the future. It is feed.

Accordingly, the present inventors have attempted to search for volatile substances that have not been found to be useful for plant-based metabolites, which have plant useful functions such as promoting plant growth and inducing disease resistance. The present invention was completed by finding a material excellent in promoting and disease resistance inducing effect and confirming its function.

It is an object of the present invention to provide a composition for promoting plant growth and controlling plant diseases comprising a volatile metabolite derived from microorganisms as an active ingredient, a method for promoting plant growth and a method for controlling plant diseases using the same.

In order to achieve the above object, the present invention provides a composition for promoting plant growth and plant disease control comprising a volatile metabolite derived from microorganisms as an active ingredient.

In another aspect, the present invention provides a method for promoting plant growth, comprising the step of treating the plant growth promoting and plant disease control composition to the target plant.

In another aspect, the present invention provides a plant disease control method comprising the step of treating the plant growth promoting and plant disease control composition to the target plant.

The present invention also provides a plant growth promoter comprising the composition for promoting plant growth and controlling plant diseases as an active ingredient.

In addition, the present invention provides a plant disease control agent comprising the composition for promoting plant growth and controlling plant diseases as an active ingredient.

P. polymyxa By developing an application technique including a method of applying five metabolites with excellent growth promoting effect and resistance inducing effect to the crops of the present invention isolated and identified from E681 bacteria, alone or in combination with plant-derived bacteria, By replacing the use of organic synthetic pesticides and fertilizers, eco-friendly agriculture can be realized, contributing to the development of agriculture and the production of safe agricultural products.

Hereinafter, the present invention will be described in detail.

The present inventors capture and identify the volatile metabolites produced by the F. E681 bacteria (KCTC 8801P) of Penicillus polymixes (KCTC 8801P) to select 17 metabolites that can be purchased in the prior art and the above 17 metabolites in the incubation conditions The effects of tobacco seedlings on growth growth and plant disease resistance were investigated. As a result, five of the 17 volatile metabolites [3-acetyl-1-propanol, 3-methyl-1-butanol] , Indole, isoamyl acetate and butyl acetate] stimulated the growth of tobacco seedlings and at the same time confirmed the effect of inducing resistance to tobacco purifying disease (see FIGS. 1 to 3). .

Since then, the growth promoting effect of the treatment of the five metabolites in greenhouse conditions was investigated in the seedlings of various plants. As a result, growth was observed in all tobacco, pepper, cucumber, cabbage and tomato seedlings (see FIGS. 4 to 9).

The present inventors observed the effect of promoting the growth of plants at the time of treatment of the five metabolites. As a result, it was confirmed that the best growth promoting results were obtained when the material was treated during the seedling development of the cotyledon in cucumber and red pepper (see FIGS. 10 and 11).

Then, the effects of the five metabolites on the induction of disease resistance of crops under greenhouse conditions were investigated. As a result, it can be seen that the ability to induce disease resistance similar to the BTH known as resistance inducing substance (see Fig. 12). In addition, the effects of bottle resistance and damage reduction under packaging conditions were investigated. As a result, it was found that the degree of fruit damage and other damages significantly decreased in anthrax of pepper (see FIG. 13).

The present inventors investigated the growth promoting effect through the mixed treatment of the metabolite and the microorganism. As a result, it can be seen that all showed an excellent growth promoting effect (see Figs. 14 to 18).

When the volatile metabolites derived from the microorganism of the present invention has excellent plant growth and plant disease control effects through the above results, and when the metabolites and microorganisms having plant growth promoting and plant disease control effects are treated together, It can be seen that the excellent effect can be expected.

The present invention provides a composition for promoting plant growth and controlling plant diseases comprising a volatile metabolite derived from microorganisms as an active ingredient.

Wherein the microorganism is Bacillus (Bacillus) and the Waist Bacillus spp (Paenibacillus) which it is preferred gram-positive bacteria including the genus bacterium, Bacillus Waist poly mix four (Penibacillus polymyxa ) (KCTC 8801P) is more preferred, but is not limited thereto.

The volatile metabolites are 3-acetyl-1-propanol, 3-methyl-1-butanol, 3-methyl-1-butanol, indole, isoamyl acetate ( Isoamyl acetate) and butyl acetate is preferably at least one selected from the group consisting of, and the active ingredient concentration of each of the volatile metabolites is preferably 10 nM to 1 mM, but is not limited thereto.

The plant is preferably a dicotyledonous plant or monocotyledonous plant, more preferably selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat, barley, but is not limited thereto.

The composition of the present invention preferably further comprises a microorganism or microbial culture medium having the effect of promoting plant growth and controlling plant diseases.

A microorganism in which the plant growth promotion and plant disease control effect ah cine Sat bakteo (Acinetobacter) genus, Agrobacterium (Agrobacterium) into, ahsseuro bakteo (Arthrobacter) in, in the azo RY rilreom (Azospirillum), Bacillus (Bacillus) Genus, genus Bradyrhizobium , genus Frankia , genus Pseudomonas , genus Rhizobium , genus Serratia , genus Paenibacillus and Thiobacillus . It is preferably selected from the group consisting of the genus, Penibacillus polymyx ( Penibacillus polymyxa ) (KCTC 8801P) is more preferred, but is not limited thereto. Carriers, other adjuvants, and the like, which are generally used in the formulation of the compositions of the present invention, may be formulated to include emulsions, suspensions, powders, granules, tablets, hydrates, water solubles, sap, flow agents, granule hydrates, aerosols, pastes, It may be formulated in various forms, such as emulsions, smokers, and the like. At this time, as a carrier used at the time of formulation, any of a solid carrier and a liquid carrier can be used depending on the purpose of use. Examples of the solid carrier include animal and vegetable powders such as starch, activated carbon, soybean meal, wheat flour, wood meal, fish meal and powdered milk; Inorganic powders such as talc, kaolin, bentonite, calcium carbonate, zeolite, diatomaceous earth, white carbon, clay, alumina, ammonium sulfate and urea. Examples of the liquid carrier include water; Alcohols such as isopropyl alcohol and ethylene glycol; Ketones such as cyclohexanone and methyl ethyl ketone; Ethers such as dioxane and tetrahydrofuran; Aliphatic hydrocarbons such as kerosine and light oil; Aromatic hydrocarbons such as xylene, trimethylbenzene, tetramethylbenzene, methylnaphthalene, and solvent naphtha; Halogenated hydrocarbons such as chlorobenzene; Acid amides such as dimethylacetamide; Esters such as glycerin esters of fatty acids; Nitriles such as acetonitrile; Sulfur-containing compounds, such as dimethyl sulfoxide, etc. are mentioned. As surfactant, For example, alkylbenzene sulfonate metal salt, dinaphthyl methane disulfonic acid metal salt, alcohol sulfate ester salt, alkylaryl sulfonate, lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkyl aryl ether, polyoxyethylene Sorbitan monoalkylate, and the like. As other auxiliaries, for example, fixing agents or thickeners such as carboxymethyl cellulose, gum arabic, sodium alginate, guar gum, tragant gum, polyvinyl alcohol, antifoaming agents such as metal soap, fatty acids, alkyl phosphates, silicones, paraffins and the like Physical property improvers, coloring agents, and the like can be used. In actual use of these preparations, they may be used as they are or may be diluted to a predetermined concentration with a diluent such as water. Application of various formulations or diluents thereof containing the composition of the present invention as an active ingredient is usually carried out by application methods, that is, spraying (for example, spraying, misting, atomizing, powder spraying, granulating spraying, sleeping) Application, permanent use, etc.), soil application (e.g. mixing, irrigation, etc.), surface application (e.g. coating, smearing, coating, etc.), dipping, poisoning, smoking, etc. Can be. The amount to be used can be appropriately determined depending on the dosage form, the damage situation, the application method, and the application place.

In another aspect, the present invention provides a method for promoting plant growth, comprising the step of treating the plant growth promoting and plant disease control composition to the target plant.

The target plant is preferably a dicotyledonous plant or monocotyledonous plant, preferably selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat, barley, but is not limited thereto.

In the above method, it is preferable that the composition further comprises a microorganism or microbial culture medium having plant growth promoting and plant disease control effects. Microorganisms in which the plant growth promotion and plant disease control effect ah Cine Saturday bakteo (Acinetobacter) genus Agrobacterium (Agrobacterium), A ahsseuro bakteo (Arthrobacter) in the azo RY rilreom (Azospirillum) genus, genus Bacillus (Bacillus) , Genus Bradyrhizobium , genus Frankia , genus Pseudomonas , genus Rhizobium , genus Serratia , genus Paenibacillus , and genus Thiobacillus . It is preferably selected from the group consisting of fungi, Penibacillus polymix ( Penibacillus polymyxa ) (KCTC 8801P) is preferably, but not limited to.

In another aspect, the present invention provides a plant disease control method comprising the step of treating the plant growth promoting and plant disease control composition to the target plant.

The target plant is preferably a dicotyledonous plant or a monocotyledonous plant, and more preferably selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat, barley, but is not limited thereto.

In the above method, the composition further comprises a microorganism or a microbial culture medium having a plant growth promoting and plant disease control effect, and the microorganism having a plant growth promoting and plant disease control effect is Acinetobacter . genus, Agrobacterium (Agrobacterium) into, ahsseuro bakteo (Arthrobacter) in the azo RY rilreom (Azospirillum) genus Bacillus (Bacillus), A bra Grundig tank emptying (Bradyrhizobium), A Franc Escherichia (Frankia) genus Pseudomonas (Pseudomonas ) Genus, Rhizobium genus, Serratia genus, Paenibacillus genus, and Thiobacillus genus, preferably selected from the group consisting of the genus, Penibacillus polymyx ( Penibacillus) polymyxa ) (KCTC 8801P) is more preferred, but is not limited thereto.

The plant disease is preferably selected from the group consisting of incurable disease, wild fire disease, anthrax, late blight, mossock disease, wilted disease, root rot disease, gray mold disease, green blight disease, leaf blight, vine cutting disease, but is not limited thereto.

The present invention also provides a plant growth promoter comprising the composition for promoting plant growth and controlling plant diseases as an active ingredient.

The plant growth promoter is preferably prepared according to a method known to those skilled in the art, and the application method thereof is generally performed in general, that is, spraying (for example, spraying, misting, atomizing, powder spraying, granulating spraying, sleeping, Etc.), soil application (e.g. mixing, irrigation, etc.), surface application (e.g. coating, smearing, coating, etc.), dipping, poisoning, smoking, etc. . The amount to be used can be appropriately determined according to the dosage form, the damage situation, the application method, the application place, and the like.

In addition, the present invention provides a plant disease control agent comprising the composition for promoting plant growth and controlling plant diseases as an active ingredient.

The plant disease control agent is preferably prepared according to a method known to those skilled in the art, and the application method thereof is generally performed in general, that is, spraying (for example, spraying, misting, atomizing, powder spraying, granulating spraying, sleeping, etc.). , Soil application, etc.), soil application (e.g. mixing, irrigation, etc.), surface application (e.g. coating, smearing, coating, etc.), dipping, poisoning, smoking, etc. have. The amount to be used can be appropriately determined according to the dosage form, the damage situation, the application method, the application place, and the like.

The plant disease is preferably selected from the group consisting of incurable disease, wild fire disease, anthrax, late blight, mossock disease, wilted disease, root rot disease, ash fungus, green blight disease, leaf blight, vine cutting disease, but is not limited thereto.

<Example 1> Isolation and Identification of Volatile Metabolites from F. E681

Volatile metabolites E681 (KCTC 8801P) were incubated in a solid medium bottle (Tryptic Soy Agar) in a stoppered small glass bottle (about 8 mm in diameter and about 3 cm in length) to capture volatile metabolites. As a result of analysis, 31 volatile metabolites were identified. 17 metabolites that are easy to handle and can be purchased from reagent companies (Sigma, USA): 3-pentanol, 3-acetyl-1-propanol , 3-methyl-1-butanol, isovaleric acid, 2-pentyl furan, 2-ethylbutyric acid ), Indole, isoamyl acetate, 3-methyl-2-butanone, 2,3-butanedione, glyoxyl Glyoxylic acid monohydrate, butyl acetate, 2-pentanone, 1-pentanol, 2-butanone, 2-methyl- 1-Propanol (2-methyl-1-propanol) and isoprene were selected and purchased from Sigma- Aldrich Corp. USA and used for plant utility assays. The material was diluted with distilled water to 10 ^-4 ~ 10 ^-8 or diluted solution of 10 nM ~ 1 mM concentration was treated to the plant.

< Example  2> by metabolites in incubation conditions Tobacco seedlings  Growth promotion and disease resistance induction effect test

Tobacco seedlings grown for 2 weeks in microtiter plates were treated with 10 ^-8 dilutions of the 17 substances, respectively, to assay the effect of plant growth and plant disease resistance.

Material treatment was performed by treating the metabolites directly with the roots, and using a partition plate ('I' shaped plate) to be spatially separated from the plant and treated by volatilization. The growth promoting effect was examined by comparing the leaf area of plants after 7 days of treatment with metabolites, and plant disease resistance inducing ability was induced by Erwinia carotovora subsp. Carotovora SCC1. After the inoculation of 10 μl (Bacterial Density = 1 × 10 ⁸ cfu / ml), the number of leaves which developed symptom after 1-2 days was examined (FIGS. 1 to 3).

As a result, five of the 17 metabolites, 3-acetyl-1-propanol, 3-methyl-1-butanol, indole, isoamyl acetate, and butyl acetate, promote plant growth and induce disease resistance. It showed a good effect in. Table 1 shows the chemical formulas and chemical structures of these five metabolite qualities. Figure 1 shows the growth promoting effect when ^10-8 dilutions of the five metabolites were treated to tobacco seedling roots. Compared to the control, distilled water treatment, four metabolite treatments showed a significant increase in leaf growth (area).

Figure 2 shows the effect of inducing resistance to tobacco purine when three dilutions of the five metabolites (10 ^-4 , 10 ^-6 , 10 ^-8 ) directly to the root and Figure 3 shows the volatilization of five metabolites When treated, it exhibits a resistance inducing effect against tobacco purpura. Compared to the control distilled water treatment, all five materials showed a significant increase in bottle resistance.

Example 3 Assay of Plant Growth Promoting Effect by Metabolites in Greenhouse Conditions

<3-1> cigarette Seedling  Growth promoting effect test

Five metabolites selected in Example 2 were diluted to three concentrations (100 μM, 1 μM, 10 μM) using distilled water and treated in tobacco seedlings. Tobacco with fully developed cotyledon ( Nicotiana tabacum cv. Xanti-nc) seedlings were transplanted to the pots, and after 2 weeks, 5 ml of each leaf was irrigated around the roots at each concentration. After 2 weeks, 5 ml was irrigated secondly. After 3 weeks, the growth of tobacco was increased, and as shown in FIG. 4, the growth was increased from as small as 8% to as large as 117% at all three concentrations of the five substances.

<3-2> pepper Seedling  Growth promoting effect test

The growth promoting effect on the pepper seedlings planted in 50 pots was tested for five metabolites selected in Example 1 in the greenhouse condition of actual pepper seedling. Five metabolites were irrigated at the root of 5 ml per week at three concentrations (1 mM, 100 μM, 10 μM) with the cotyledons developed 11 days after sowing. Two weeks later, the same amount of material was subjected to secondary treatment. After 9 days, the height was examined, and as shown in FIG. 5, all of the five materials showed excellent growth promoting effects of 27% -42% compared to the control.

<3-3> cucumber Seedling  Growth promoting effect test

Experiments with pots in greenhouses. Each metabolite of 100 μM concentration was irrigated with 5 ml of root per week when cucumber cotyledon developed, and after 2 weeks, 10 ml was irrigated twice and growth was again examined after 2 weeks (FIG. 6). As a result, as shown in Figure 6, most of the metabolites in the grass, leaf, hard diameter, and above-ground living body showed the growth promoting effect, and in the case of live weight increased from 13% to 45%. On the other hand, the growth promotion results when the 10 μM concentration of each metabolite was treated in the same manner is shown in Figure 7 can be seen that the height increased in all five materials.

<3-4> Chinese cabbage Seedling  Growth promoting effect test

Each metabolite at 100 μM concentration was irrigated 20 ml at the root of cabbage seedlings with fully developed cotyledons, and after 2 weeks, 20 ml was irrigated twice and growth was examined after 2 weeks again (FIG. 8). As a result, as shown in Figure 8, most of the metabolites in the leaf, leaf, rigid diameter and above-ground living body showed the growth promoting effect, the small diameter increased from 5% to 39%

<3-5> tomato Seedling  Growth promoting effect test

Each metabolite of 100 μM concentration was irrigated with 20 ml of root per week of tomato seedlings with fully developed cotyledon. After 2 weeks, 20 ml was irrigated twice and growth was examined after 2 weeks (FIG. 9). As a result, as shown in FIG. 9, four kinds of materials showed growth promoting effect in the grass length, the leaf length and the ground part, and the growth was increased from 13% to 22%.

Example 4 Growth Promoting Effect of Plants by Treatment Time of Metabolites

<4-1> Test for growth promoting effect of cucumber

Diluted solution of 10 μM of the five materials selected in Example 2 was subjected to cotyledon development on cucumbers under greenhouse conditions, and then treated on the roots with one leaf and three leaves. 5 ml of each dilute solution was first treated at each time period, and after 2 weeks of 10 ml of each treatment, the plant growth was examined after 2 weeks (FIG. 10). As a result, the measured value of the control group treated with distilled water was set to 100, and the relative value thereof is shown in FIG. 10, but treatment of the material during the seedling development of the cotyledon showed the best effect.

<4-2> Growth Promotion of Red Pepper

A 10 μM dilution solution of the five materials selected in Example 2 was treated to the roots after the development of the cotyledon and the five main leaves of pepper in greenhouse conditions. 5 ml of each diluting solution was first treated at each time period, and 10 ml of 10 ml each were treated after 2 weeks, and the growth of the plant was examined after 2 weeks (FIG. 11). As a result, the relative value of the control group treated with distilled water was set to 100, and the relative value thereof is shown in FIG.

<Example 5> Induction effect of disease resistance of crops by metabolites in greenhouse conditions

Five kinds of materials selected in Example 2 were tested for the effect of inducing disease resistance against tobacco wild pathogen ( Pseudomonas syringae pv. Tabaci ). Tobacco was sown in pots, and after 5 weeks of emergence, each diluting solution (1 μM, 10 μM, 100 μM, 1 mM) was irrigated with 5 ml of tobacco seedlings per week. As a control, BTH (benzo- (1,2,3) -thiadiazole-7-carbothioic acid S -methyl ester), an untreated and resistant inducer, was treated in the same amount at 300 uM. Three days after metabolite treatment, pathogen dilutions (10 ⁵ cfu / ml) were inoculated onto the leaves and three days after inoculation, the pathogen density within 1 cm 2 of the leaves was examined (FIG. 12). As a result, as shown in Figure 12, 3-methyl-1-butanol and indole (indole) showed a similar level of disease resistance to control BTH.

< Example  6> Anthrax and Other Damages of Red Pepper by Metabolites in Packaging Conditions Reduction  Effect black

Among the red pepper seedlings grown in Example 3-2, 100 μM-isoamyl acetate treated seedlings and butyl acetate-treated seedlings were transplanted into the experimental packaging. The pesticide treatment was carried out on the ground. As a result of investigating the fruit damage caused by the yield and disease after pepper harvesting, there was no significant difference in yield, but as shown in FIG. 13, the degree of fruit damage and other damages caused by anthrax were significantly reduced. On the other hand, the treatment of the F. bacilli also showed a good effect as expected. From this result, the metabolite that shows good effect in the early stage of growth of red pepper seedlings and the F. bacilli which are considered to be effective in the late stage of growth. Dosing is expected to produce good results.

< Example  7> Cucumber by mixing 2 kinds of metabolites Seedling  Growth promoting effect test

Two metabolites were mixed to each 100 μM concentration, and 20 ml of irrigation of roots of cucumber seedlings with fully developed cotyledon was irrigated. After 2 weeks, 20 ml of irrigation was performed. (FIG. 14). As a result, as shown in FIG. 14, all mixtures showed growth-promoting effects in the grass length, the leaves, and the ground part, and the growth was increased from 10% to 30%. 15 is a photograph showing growth growth effects of six kinds of two mixtures.

< Example  8> Tomato by Mixing Microorganisms and Metabolites Seedling  Growth promoting effect test

The cotyledons were fully treated with 20 ml of 100 μM metabolite and 50 ml of Penibacillus polymyx E681 (Bacterial Density = 10 ⁷ cfu / ml) in roots of tomato seedlings fully developed. 16). As a result, as shown in Figure 16 showed excellent growth promoting effect in the grass length and above-ground live weight, and in the above-ground live weight was increased from as small as 32% to as large as 46% in the four mixtures.

< Example  9> 3 crops by mixing 2 kinds of microorganism and metabolite Seedling  Growth promoting effect test

Two metabolites and F. Enifolia F681 were treated together at the root of each seedling of three crops with fully developed cotyledons. The two metabolites were indole and isoamyl acetate, and each 10 ml of 100 μM solution was mixed with 50 ml of F. E681 (Bacterial Density = 10 ⁷ cfu / ml). Growth was examined after weeks (FIG. 17). As a result, as shown in Figure 17 all three crops showed an excellent growth promoting effect and in the case of the ground live weight increased 19% -33% depending on the crop. 18 is a photograph showing the tomato growth promoting effect by the microorganism-metabolite mixing treatment.

1 is a photograph showing the growth promoting effect when the metabolite was treated to tobacco seedling root in the culture vessel.

Figure 2 is a graph showing the effect of resistance to tobacco smoke when treated with metabolic roots.

Figure 3 is a graph showing the effect of resistance to tobacco smoke when the metabolites were volatilized in the culture vessel.

Figure 4 is a graph showing the growth promoting effect of tobacco seedlings by the metabolite treatment.

5 is a graph showing the growth promoting effect of pepper seedlings by the metabolite treatment.

6 is a graph showing the growth promoting effect of cucumber seedlings by the metabolite treatment.

7 is a photograph showing the growth promoting effect of cucumber seedlings by the metabolite treatment.

8 is a graph showing the growth promoting effect of Chinese cabbage seedlings by the metabolite treatment.

9 is a graph showing the growth promoting effect of tomato seedlings by the metabolite treatment.

Figure 10 is a graph showing the growth promoting effect of each treatment time for the metabolite cucumber.

Figure 11 is a graph showing the growth promoting effect of the treatment period for the pepper of the metabolite.

12 is a graph showing the effect of resistance to tobacco wildfire by treatment of metabolites.

Figure 13 is a graph showing the effect of reducing the anthrax and other damage of red pepper by the treatment of F.

14 is a graph showing the growth promoting effect of cucumber seedlings by mixing two metabolites.

It is a photograph which shows the growth promoting effect of cucumber seedling by the mixing process of two metabolites.

Fig. 16 is a graph showing the growth promoting effect of tomato seedlings by the mixing treatment of F. phylbacilli polymycobacteria and one metabolite.

FIG. 17 is a graph showing the growth promoting effect of three crop seedlings by the mixing treatment of two F.

Fig. 18 is a photograph showing the growth promoting effect of tomato seedlings by the mixing treatment of the F. bacilli and metabolite.

Claims (23)

Composition for promoting plant growth and controlling plant diseases comprising volatile metabolites derived from microorganisms as an active ingredient. According to claim 1, wherein the microorganism is a Gram-positive bacterium comprising Bacillus ( Bacillus ) and Paenibacillus (bacteria) bacteria, plant growth promotion and plant disease control composition. The method of claim 1, wherein the microorganism is Penibacillus polymyxa (KCTC 8801P) composition for promoting plant growth and plant disease control composition. The method of claim 1, wherein the volatile metabolite is 3-acetyl-1-propanol, 3-methyl-1-butanol, 3-methyl-1-butanol, indole ), Isoamyl acetate and butyl acetate (butyl acetate) is a composition for promoting plant growth and plant disease control, characterized in that at least one member selected from the group consisting of. 5. The composition for promoting plant growth and controlling plant diseases according to claim 4, wherein the concentration of the active ingredient of each of the volatile metabolites is 10 nM to 1 mM. The composition for promoting plant growth and controlling plant diseases according to claim 1, wherein the plant is a dicotyledonous plant or monocotyledonous plant. The composition for promoting plant growth and controlling plant diseases according to claim 6, wherein the plant is selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat and barley. The composition for promoting plant growth and controlling plant diseases according to claim 1, further comprising a microorganism or a microbial culture medium having plant growth promoting and plant disease control effects. The method of claim 8, wherein the microorganism in which the plant growth promotion and plant disease control effect ah cine Sat bakteo (Acinetobacter) genus, Agrobacterium (Agrobacterium) into, ahsseuro bakteo (Arthrobacter) in the azo RY rilreom (Azospirillum) in , Genus Bacillus , genus Bradyrhizobium , genus Frankia , genus Pseudomonas , genus Rhizobium , genus Serratia , genus Paenibacillus , and The composition for promoting plant growth and controlling plant diseases, which is selected from the group consisting of the genus Thiobacillus . 10. The composition for promoting plant growth and controlling plant diseases according to claim 9, wherein the microorganism having the effect of promoting plant growth and controlling plant diseases is Penibacillus polymyxa (KCTC 8801P). A method for promoting plant growth, comprising treating the plant with the composition for promoting plant growth and controlling plant diseases of claim 1. The method of claim 11, wherein the plant of interest is a dicotyledonous or monocotyledonous plant. The method of claim 12, wherein the plant of interest is selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat, barley. The method according to claim 11, wherein the composition further comprises a microorganism or a microbial culture medium having plant growth promoting and plant disease control effects. The method of claim 14, wherein the microorganism in which the plant growth promotion and plant disease control effect ah cine Sat bakteo (Acinetobacter) genus, Agrobacterium (Agrobacterium) into, ahsseuro bakteo (Arthrobacter) in the azo RY rilreom (Azospirillum) in , Genus Bacillus , genus Bradyrhizobium , genus Frankia , genus Pseudomonas , genus Rhizobium , genus Serratia , genus Paenibacillus , and Method for promoting plant growth, characterized in that it is selected from the group consisting of the genus Thiobacillus . The method of claim 15, wherein the microorganism having the effect of promoting plant growth and controlling plant diseases is Penibacillus polymyx ( Penibacillus polymyxa ) (KCTC 8801P) method for promoting plant growth. The plant disease control method comprising the step of treating the plant growth promoting and plant disease control composition of claim 1 to the target plant. 18. The method for controlling plant diseases according to claim 17, wherein the target plant is a dicotyledonous plant or monocotyledonous plant. The method of claim 18, wherein the target plant is selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat, and barley. 18. The method for controlling plant diseases according to claim 17, further comprising a microorganism or a microbial culture medium having an effect of promoting plant growth and controlling plant diseases in addition to the composition. The method of claim 20, wherein the microorganism in which the plant growth promotion and plant disease control effect ah cine Sat bakteo (Acinetobacter) genus, Agrobacterium (Agrobacterium) into, ahsseuro bakteo (Arthrobacter) in the azo RY rilreom (Azospirillum) in , Genus Bacillus , genus Bradyrhizobium , genus Frankia , genus Pseudomonas , genus Rhizobium , genus Serratia , genus Paenibacillus , and Plant disease control method characterized in that it is selected from the group consisting of the genus Thiobacillus . 22. The method of claim 21, wherein the microorganism having an effect of promoting plant growth and controlling plant diseases is Penibacillus polymyx ( Penibacillus polymyxa ) (KCTC 8801P) plant control method characterized in that. 18. The plant disease according to claim 17, wherein the plant disease is selected from the group consisting of incurable disease, wild fire disease, anthrax disease, late blight, mozzarella disease, wilted disease, root rot disease, gray mold disease, green blight disease, leaf blight disease, and vine cutting disease. Control method.

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