KR101313900B1 - Methods for plant protection by fusaricidin - Google Patents

Abstract

The present invention relates to a plant protection method using a microorganism-derived metabolite, and in particular, it is effective to use fusaricidin, a salt thereof, or a derivative thereof, which is a lipopeptides-based substance produced by Paenibacillus polymyxa . It relates to a plant disease resistance inducer containing as a component. Fuzacidin of the present invention exhibits the effect of preventing the plant disease by making it resistant to plant diseases in plants, more excellent plant disease inhibition at a concentration 1000 times lower than the conventional plant disease control effect by the antibiotic effect Since it is effective, it can be usefully used as a plant disease resistance inducer, fuzisidine of the present invention is obtained biologically, not chemically synthesized, replacing and the use of organic synthetic pesticides to control and prevent plant diseases in environmentally friendly agriculture Can be applied to

Description

Method for plant protection by fuzacidin {Methods for plant protection by fusaricidin}

The present invention relates to a plant disease resistance inducer containing a metabolite of a microorganism as an active ingredient and to a plant disease resistance induction method using the method.

The securing of food is becoming more and more important due to factors such as population increase and disturbance of ecosystem due to climate change.In this regard, sustainable eco-friendly agriculture and safe agricultural production and environmentally friendly pesticides and fertilizers are required. In recent years, the importance of developing agricultural materials has increased greatly. One of them is developing safe and efficient ways to control plant diseases, which are a major obstacle to growing crops. As a method of replacing or supplementing chemical synthetic pesticides, many methods for controlling plant diseases using functional microorganisms have been developed, and some have entered the commercialization stage, but the application efficiency in the field is lower than that of chemical pesticides, or Disadvantages such as falling are frequently raised, which makes it difficult to improve utilization. In order to overcome this problem, an attempt has been made to find and use a microorganism-derived functional material in addition to using the microorganism itself.

In the rhizosphere and soil, various microorganisms coexist in complex interrelationships, and the types of microorganisms living in relation to plants may vary according to the type of plant and the soil environment. In terms of microorganisms, the target plants may be affected by the materials and other properties they produce. It is very important to secure various functional metabolites because there are many kinds of plants and phytopathogens causing problems also vary, and the disease resistance inducers may have different effects. Such disease resistance inducing substances may be utilized in various ways, either alone or in combination with other functional substances and microorganisms, or with chemically synthetic pesticides.

Fusaricidin is a Paenibacillus Polymyxa ) is an antibiotic isolated from the strain 6 amino acid residues form a ring structure and contains 15-guanidino-3-hydroxypentadecanoic acid (see Fig. 1). . To this day, fujarycidin LI-F03, LI-F04, LI-F05, LI-F07 and LI-F08 have been isolated and identified from Penicillus polymixes (Kurusu K, Ohba K, Arai T and Fukushima K). J. Antibiotics 40: 1506-1514, 1987), fuzisidine A, B, C and D have also been reported (Kajimura Y and Kaneda M. J. Antibiotics 49: 129-135, 1996; Kajimura Y and Kaneda M. J. Antibiotics 50: 220-228, 1997). The amino acid chains of fuzacidin are not encoded by genes and synthesized by ribosomes, as are common polypeptides, but by non-ribosomal peptide synthetase (NRPS) (Marahiel MA, Stachelhaus T and Mootz HD). Chem . Rev. 97: 2651-2673, 1997; Doekel S and Marahiel MA. Metab . Eng . 6: 64-77, 2001). The present inventors have already discovered fuzisidine biosynthesis genes through the genome detoxification study of the E. F. E681 strain of Penivacillus polymyx and revealed their function (Choi S.-K. et. al . 2007. Biochem . and Biophys . Res. Commun . 365 : 89-95).

The research to search for plant-derived substances in the metabolites of microorganisms has been conducted by various research teams for a long time, but it is not often used for agriculture. In the actual crop production system, technology that can improve the resistance of plants to diseases, insects or environmental stress in small amounts as a metabolite derived from microorganisms, rather than the conventional organic synthetic pesticides, is an important factor in the development of eco-friendly agriculture.

Accordingly, the present inventors have shown that fuzacidin, a lipopeptide antibiotic, among the metabolites produced by the F. polymycobacterium, has the effect of inhibiting red pepper blight and bacterial urinary disease in red pepper seedlings and cucumbers in cucumber seedlings at low concentrations. It was confirmed that the plant had excellent disease control effects by making plants resistant to disease at concentrations lower than 1 / 100-1 / 1000 than the conventional antibiotic control effect. The present invention has been completed by revealing that it can be usefully used.

On the other hand, due to the nature of inducible resistance in plants, fuzisidine can be used as a control for various plant diseases such as viruses, fungi and bacteria.

An object of the present invention is to identify the usefulness of the fusicidin, which is a metabolite produced by the F. bacterium polymyx bacteria, to plants, and to secure the technology of producing fuzacidin using this strain, and to effectively utilize the metabolite. It is to provide a plant disease resistance inducer comprising a component and a plant disease resistance induction method using the same.

In order to achieve the above object, the present invention provides a resistance inducer to plant diseases containing fusaricidin, salts thereof, or derivatives thereof as an active ingredient.

The present invention also provides a method of inducing resistance to plant diseases, comprising the step of treating fuzacidine, or a salt thereof, or a derivative thereof in a plant, its seed or its habitat.

Fuzacidin of the present invention exhibits the effect of preventing the plant disease by making it resistant to plant diseases in plants, more excellent plant disease inhibition at a concentration 1000 times lower than the conventional plant disease control effect by the antibiotic effect It can be useful as a plant disease resistance inducer because it exhibits the effect, fujarysidine of the present invention is obtained biologically, not chemically synthesized, replacing and using organic synthetic pesticides to control and prevent plant diseases in environmentally friendly agriculture Can be applied to

1 is a diagram showing the chemical structure of fuzacidin A.
Figure 2 is a graph showing the control effect on bacterial spot pattern disease when irrigating fuzacidine in pepper seedlings.
Figure 3 is a diagram showing the control effect against pepper blight when irrigation of red pepper seedlings in pepper seedlings.
Figure 4 is a figure showing the control effect against the soft beetle when fusicidine injected in cucumber seedlings.

Hereinafter, the present invention will be described in detail.

The present invention provides a resistance inducer to plant diseases, containing fusaricidin, salts thereof, or derivatives thereof as an active ingredient.

The inducer of resistance to plant diseases preferably comprises fuzacidin, salts thereof, or derivatives thereof from 10 ng / ml (0.01 ppm) to 16 µg / ml (16 ppm), and 10 ng / ml (0.01). ppm) to 1 μg / ml (1 ppm), more preferably 10 ng / ml (0.01 ppm) to 1 μg / ml (1 ppm), but are not limited thereto.

The fuzacidin is preferably LI-F04a (fusaricidin A), LI-F04b (fusaricidin B), LI-F05a, LI-F05b, LI-F08a and LI-F08b, but LI-F04a, LI Any one or more components selected from the group consisting of -F04b, LI-F05a, LI-F05b, LI-F08a and LI-F08b can be used as an inducer of resistance to plant diseases of the present invention.

The fuzacidin is Paenibacillus polymyxa ) is preferably isolated from strain E681, but is not limited thereto.

The plant is preferably a dicotyledonous plant or monocotyledonous plant, more preferably selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat, barley, but is not limited thereto.

The plant disease is preferably selected from the group consisting of late blight, inflorescence, bacterial spot pattern disease, wild fire disease, anthrax disease, mozzarella disease, wilting disease, root rot disease, gray mold disease, green blight disease, leaf blight disease, vine cutting disease, but is not limited thereto. .

Inducers resistant to plant diseases of the present invention may be used as agrochemically acceptable carriers, in addition to other conventional synthetic pesticides or biopesticides having an effect of inhibiting or controlling plant diseases, or mixed with fertilizers.

In a specific embodiment of the present invention, fuzacidin was obtained from three-step purification of butanol extraction, silica gel chromatography, and Sephatex column chromatography from the Panicivacillus polymix E681 strain. It was confirmed by HPLC and mass spectroscopy that the fuzacidin is present in the six fuzacidins of LI-F04a, LI-F04b, LI-F05a, LI-F05b, LI-F08a and LI-F08b. See FIG. 1). When the fusalicidin obtained above was treated to red pepper seedlings, it showed inhibitory effect on bacterial spot pattern pathogens and red pepper blight germ at low concentrations, and when it was treated to cucumber seedlings, it showed an inhibitory effect on cucumber softwood disease at low concentrations (Table 2). , Table 3 and FIGS. 2-4).

In addition, in a specific embodiment of the present invention, as a result of confirming the minimum inhibitory concentration for the main phytopathogens of the fusicidin, the plant disease of the pepper seedlings and cucumber seedlings confirmed at a concentration of less than that showing an antibiotic effect at 16 ppm or more The inhibitory effect was not due to antibiotic effect but to resistance induction (see Table 4).

Thus, fujarysidine of the present invention induces resistance to plant diseases against plants at low concentrations, and shows more excellent plant disease suppression effect at a concentration 1000 times lower than the plant disease control effect by antibiotic effect It has been found to be useful as an inducer for disease resistance and can be applied to environmentally friendly farming methods.

Provided as a strain capable of efficiently producing fuzacidine, an active ingredient of the resistance inducing agent of the present invention, the F. nivicillus polymixes E681 strain, but is not limited thereto. The present inventors used tryptic soy broth (TSB) to which 3% of glucose was added for the cultivation of the F. E681 strain of Pa., But used conditions of shaking culture at 30 ° C. for 2-3 days, but is not limited thereto. Three-stage method of butanol extraction (n-BuOH extract), silica gel column flash chromatography and sephadex LH-20 column chromatography However, it is not limited to this method.

Inducing agents resistant to plant diseases of the present invention can be used alone or dissolved in distilled water, or formulated with other excipients can be used as resistance inducing plants against plant diseases. Carriers, other adjuvants, and the like, which are generally used in the formulation of the compositions of the present invention, may be formulated to include emulsions, suspensions, powders, granules, tablets, hydrates, water solubles, sap, flow, granule hydrates, aerosols, pastes, It may be formulated in various forms such as emulsions. At this time, as a carrier used for formulation, any of a solid carrier and a liquid carrier can be used depending on the purpose of use. Examples of the solid carrier include animal and vegetable powders such as starch, activated carbon, soybean meal, wheat flour, wood meal, fish meal and powdered milk; Inorganic powders such as talc, kaolin, bentonite, calcium carbonate, zeolite, diatomaceous earth, white carbon, clay, alumina, ammonium sulfate and urea. Examples of the liquid carrier include water; Alcohols such as isopropyl alcohol and ethylene glycol; Ketones such as cyclohexanone and methyl ethyl ketone; Ethers such as dioxane and tetrahydrofuran; Aliphatic hydrocarbons such as kerosine and light oil; Aromatic hydrocarbons such as xylene, trimethylbenzene, tetramethylbenzene, methylnaphthalene, and solvent naphtha; Halogenated hydrocarbons such as chlorobenzene; Acid amides such as dimethylacetamide; Esters such as glycerin esters of fatty acids; Nitriles such as acetonitrile; Sulfur-containing compounds, such as dimethyl sulfoxide, etc. are mentioned. As surfactant, For example, alkylbenzene sulfonate metal salt, dinaphthyl methane disulfonic acid metal salt, alcohol sulfate ester salt, alkylaryl sulfonate, lignin sulfonate, polyoxyethylene glycol ether, polyoxyethylene alkyl aryl ether, polyoxyethylene Sorbitan monoalkylate, and the like. As other auxiliaries, for example, fixing agents or thickeners such as carboxymethyl cellulose, gum arabic, sodium alginate, guar gum, tragant gum, polyvinyl alcohol, antifoaming agents such as metal soap, fatty acids, alkyl phosphates, silicones, paraffins and the like Physical property improvers, coloring agents, and the like can be used. In actual use of these preparations, they may be used as they are or may be diluted to a predetermined concentration with a diluent such as water.

Throughput of the resistance inducer to plant diseases according to the present invention is a range that does not deviate significantly from the conventional pesticide throughput, for example, sprayed until the inhibitor solution evenly wetted down the surface of the plant. Moreover, you may process at any stage of growth from a seed of a plant to maturity. The resistance inducer of the present invention can be treated by conventional pharmaceutical treatment methods such as spraying, irrigation or dipping.

In the present invention, the amount of the inducer resistant to plant diseases may be appropriately adjusted according to the type of plant to be treated, the type and condition of the plant disease, treatment time and method, and the like.

Inducing agents resistant to plant diseases of the present invention can be used for the treatment or prevention of infectious plant diseases caused by various bacterial organisms. Erwinia carotovora of vegetable purifying disease, Erwinia araliavora of ginseng root disease, Pseudomonas cepacia of sesame seed disease, Ralstonia solanacearum of red pepper and tomato green blight (Ralstonia solanacearum), Xanthomonas citri of citrus ulcers, Xanthomonas oryzae of rice blight, or Xanthomonas campestris of vegetable rot. Infections with Gram-negative bacteria, including Agrobacterium tumefaciens infections of myocarcinoma tumors .

The present invention also provides a method of inducing resistance to plant diseases, comprising the step of treating fuzacidine, or a salt thereof, or a derivative thereof in a plant, its seed or its habitat.

Fuzacidine of the present invention can be usefully used in a method of inducing plant disease resistance to plants at low concentrations to induce resistance to plant diseases.

The fuzacidin is preferably LI-F04a, LI-F04b, LI-F05a, LI-F05b, LI-F08a and LI-F08b, but LI-F04a, LI-F04b, LI-F05a, LI Any one or more components selected from the group consisting of -F05b, LI-F08a and LI-F08b can be used as inducers of resistance to plant diseases of the present invention.

The target plant is preferably a dicotyledonous plant or monocotyledonous plant, preferably selected from the group consisting of tobacco, cucumber, pepper, tomato, cabbage, wheat, barley, but is not limited thereto.

The plant disease is preferably selected from the group consisting of late blight, inflorescence, bacterial spot pattern disease, wild fire disease, anthrax disease, mozzarella disease, wilting disease, root rot disease, gray mold disease, green blight disease, leaf blight disease, vine cutting disease, but is not limited thereto. .

The content of fuzacidine, salts thereof, or derivatives thereof used in the method of the present invention may be appropriately adjusted depending on the type of plant, the type and condition of plant diseases, the treatment time and method, etc., but it is usually 10 ng / ml (0.01). ppm) to 16 μg / ml (16 ppm), more preferably 10 ng / ml (0.01 ppm) to 1 μg / ml (1 ppm), and 10 ng / ml (0.01 ppm) ) To 1 μg / ml (1 ppm), but is not limited thereto.

In the above method, the composition further comprises a microorganism having the effect of promoting plant growth and controlling plant diseases, and the microorganism having the effect of promoting plant growth and controlling plant diseases is Acinetobacter genus, Agro. tumefaciens (Agrobacterium) into, ahsseuro bakteo (Arthrobacter) in the azo RY rilreom (Azospirillum) genus Bacillus (Bacillus), A bra Grundig tank emptying (Bradyrhizobium), A Franc Escherichia (Frankia) genus Pseudomonas (Pseudomonas), A It is preferably selected from the group consisting of the genus Rhizobium , Serratia , Paenibacillus and Thiobacillus , and more preferably Penibacillus polymyxa . However, it is not limited thereto.

In the application of the above method, a method generally performed generally, that is, spraying (for example, spraying, misting, atomizing, powder spraying, granulating spraying, sleeping, normal, etc.), soil application ( For example, mixing, irrigation, etc.), surface application (for example, coating, smearing, coating, etc.), dipping, poisoning, etc. can be performed, but it is not limited to this. The amount to be used can be appropriately determined according to the dosage form, the damage situation, the application method, the application place, and the like. In the above method, it may further be formulated or mixed with other conventional biopesticides, synthetic pesticides, or fertilizers.

The plant disease is preferably selected from the group consisting of incurable disease, wild fire disease, anthrax, late blight, mossock disease, wilted disease, root rot disease, ash fungus, green blight disease, leaf blight, vine cutting disease, but is not limited thereto.

[ Example ]

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

< Example  1> Fanny Bacillus Polyamic acid  From bacteria De Perez of Dein  Isolation and identification

The present invention was used by the inventors of the present invention Professor Chang-Seok Park (Patent Bacillus polymixes E681) as a plant-utilized bacterium (Patent No. 0220402, deposited strain No .: KCTC 8801P, the current patent is expired). At the time of deposit of the strain is Bacillus miksa poly (Bacillus polymyxa ) E681, but the classification system newly introduced by Ash C. et al (Ash, C. et al . , 1993. Antonie van Leeuwenhoek . 64: 253-260) was applied to the genus Paenibacillus ( Penbacillus ) genus was renamed to the current strain name 'Panibacillus polymixes E681'. The strain was shaken and cultured at 30 ° C. for 3 days using tryptic soy broth (TSB) added with 3% glucose, and the culture solution was extracted with butanol to prepare a sample about 10-fold concentrated. To confirm the production of the target metabolite (fuzacidin) in the culture medium, Fusarium , a phytopathogenic fungus, Fusarium , was used as a paper disk method. oxysporum ) and Gram-positive bacteria Micrococcus luteus ), the growth inhibition activity assay was confirmed that the excellent activity, that is, the growth inhibition ring. Method for butanol extraction (n-BuOH extract), silica gel column flash chromatography (Merk) and sephadex LH-20 column chromatography (Pharmacia) for the separation and purification of fuzacidine Was used. This three-stage purification process was used to obtain fuzacidin samples and six fucicidins in the sample through an LC / MS analyzer (Thermo Electron Co.) equipped with a reversed phase column (X-Terra, Waters Co.). Revealed that it is mixed. This fuzacidin mixed sample was fractionated from six fuzacidins via a HPLC (Shimadzu Co.) instrument equipped with a C-18 reverse phase column (YMC Co.). The mobile phase used was 32% acetonitrile. The molecular weights of the six species were 883, 897 (two species), 911 (two species) and 925 Daltons, respectively. Sidine samples were hydrolyzed by the pico-tag method and the amino acid composition was analyzed by HPLC instrument (Waters Co.) equipped with a Pico-tag column (Waters Co.). These correspond to the fusaricidin A, fusaricidin B, LI-F5a, LI-F5b, LI-F8a, and LI-F8b of the fusicidin A reported in the literature. Confirmed.

< Example  2> On pepper seedlings  About De Perez of Dein  Disease resistance induction effect test

The inventors of fuzacidin While studying the usefulness of plants, in addition to the known antimicrobial activity against phytopathogenic microorganisms, the fact that induce the disease resistance of plants at low concentrations was found through the following experiment.

<2-1> De Perez of Dein Pepper seedling In cross-processing  by Bacterial spot disease  Control effect

Fuzacidine samples (six kinds of mixtures) obtained in Example 1 were dissolved and diluted with methanol and sterile distilled water, respectively, to give 10 ng / ml (= 0.01 ppm) and 1 μg / ml (= 1 ppm). Dilutions of concentration were prepared. When the main leaves of red pepper ('Bujiang' variety) seedlings were 3-4 sheets, six kinds of dilution samples (experimental group) prepared above were irrigated on the pepper roots. As a positive control, a strain of Paenibacillus polymyxa E681 (Bacterial density, 1x10 ⁸ cfu / ml) and BTH [benzo- (1,2,3) -thiadiazole-7-carbothioic acid S-methyl ester, 1.0 mM ], As a negative control, the mutant strain ( fuel density, 1 × ^{10 8} cfu / ml) and sterile distilled water in which fuzacidine biosynthetic enzyme gene ( fusA ) was inactivated was irrigated in the same manner as the experimental group.

As a result, after 1 week of treatment, bacterial spot pattern pathogens ( Xanthomonas axonopodis pv. vesicatoria (even density, 1x10 ⁶ cfu / mL) was injected into the pepper leaves and the immune response was observed. Both the concentrations of 10 ng / mL and 1 ㎍ / mL showed inhibitory effects. At lower concentrations, the effect was better, which was about the same as when the P. polymyxa E681 bacteria were irrigated (Fig. 2). Therefore, in the case of the mutant strains in which the fuzacidin gene is inactivated, the effect of inhibiting the disease is considerably lowered.

<2-2> De Perez of Dein Pepper seedling Foliar  By spraying Pepper Plague  Control effect

Fuzisidine samples obtained through the purification process from the three-stage purification process of fuzacidin of Example 1 to the silica gel chromatography in two stages were dissolved and diluted with methanol and sterile distilled water to obtain 0.1, 1.0, Dilutions of 10, 100, 500 and 1000 ppm concentrations were prepared respectively. As a control drug, dimesomorph (1 ppm concentration), which is a caybean germ disease chemical agent, was used.

The causative bacterium of cayenne pepper is Phytophthora capsaisi, 217Phytophthora capsici 217) strain was used. After incubating the specimen for 7 days in V-8 juice, the colony of the cultured late blight was rinsed five times with sterile distilled water to remove the extra V8 medium, and then distilled water was added to each 10 ml per petri dish at room temperature under fluorescent light. The cultured saccharin formed by culturing for 1 day at was stored at 4 ° C. before the test and used for the experiment.

Pepper seeds ('Manita' varieties) were surface sterilized with a 1% solution of Na-hyperchloride, and then seeded at seedlings for 24 hours at 30 ° C. Two weeks after germination, they were transplanted into flora guard (TKS 2) topsoil and grown. Plasma seedlings were irrigated with soil at a concentration of 1.0 × 10 ⁶ cystospore / ml for 2 weeks. The previously prepared fuzacidin sample and the control dimethomorph were sprayed on the leaves of pepper seedlings before and after the late blight irrigation.

As a result of examining the prophylactic and therapeutic effect of fujarycidin against pepper blight through the experiments, as shown in Table 1, in the pretreatment rather than the post-treatment, when treated at a lower concentration than the high concentration, particularly at 1 ppm, excellent control effect Indicated. From the above results, it can be seen that the control effect of fuzacidin was not caused by antibacterial action but by resistance induction. The effect of high concentrations of 1000 ppm may be attributed to its function as an antibiotic rather than to induce resistance.

Effect of Fusarisidine Foliar Spray on Red Pepper Disease process Intensity of disease symptoms (%) Diseased plants (%) Pretreatment After treatment Pretreatment After treatment Control 1 (No Treatment) 83.3 81.7 100.0 100.0 Control 2 (Dimesomorph, 1 ppm) 0.0 33.3 0.0 33.3 Fuzacidin 0.1 ppm 45.0 40.0 66.7 50.0 Fuzacidin 1 ppm 6.7 78.3 50.0 100.0 Fuzacidin 10 ppm 35.0 75.0 83.3 100.0 Fuzacidin 100 ppm 48.3 91.7 83.3 100.0 Fuzacidin 1000 ppm 35.0 78.3 50.0 83.3

<2-3> De Perez of Dein Pepper seedling In cross-processing  by Pepper Plague  Control effect

In the same manner as in Example 2-2, fuzaridin samples, pepper blight and pepper seedlings were prepared, and fuzacidine samples were first irrigated to pepper seed roots before irrigation of pepper blight.

As a result, the results of FIG. 3 and Table 2 were obtained. As in Example 2-2, the best control effect was obtained at the concentration of 1 ppm, and the effect from the high concentration of 1000 ppm was judged to be due to its function as an antibiotic rather than to induce resistance.

Control of Pepper Blight Disease by Fusarisidine Irrigation process Intensity of disease symptoms (%) Diseased plants (%) Control 1 (No Treatment) 83.3 100.0 Control 2 (Dimesomorph, 1 ppm) 3.3 33.3 Fuzacidin 0.1 ppm 53.3 83.3 Fuzacidin 1 ppm 3.3 33.3 Fuzacidin 10 ppm 36.7 50.0 Fuzacidin 100 ppm 56.7 100.0 Fuzacidin 1000 ppm 36.7 66.7

<2-4> De Perez of Dein In lobe injection  Cucumber purifying disease control effect

As bacterial bacillus, E. carotovora SCC1 strain, which was preserved by the National Academy of Agricultural Science, was incubated in LB agar for 24 hours and then suspended in sterile distilled water at a concentration of 1x10 ⁸ cfu / ml and used for plant inoculation. Cucumber ('Silver white tea cultivar') was transplanted to floraguard (TKS 2) soil mixed with 10% perite after surface sterilization with 1% Na-hypercholoride and seeded for 2 weeks, and then prepared in the same manner as in Example 2-2. Zaricidin samples were injected into the lamellae, respectively. After 1 week, E. carotovora SCC1 was inoculated with foliar spray, and disease development was observed until 2 weeks later.

As a result, as shown in Table 3, it showed the best control effect at the low concentration of 0.1 ppm, the effect from the high concentration of 100 ppm is judged to be due to the function as an antibiotic rather than resistance induction.

Effect of Cucumber Follicle Injection on Cucumber Soft Disease process Intensity of disease symptoms (%) Control 1 (No Treatment) 66.3 Control 2 (BTH, 0.1 mM) 43.1 Fuzacidin, 0.1 ppm 27.5 Fuzacidin, 1 ppm 56.4 Fuzacidin, 10 ppm 81.3 Fuzacidin, 100 ppm 34.4

< Example  3> De Perez of Dein  main Phytopathogenic bacteria  Minimum inhibitory concentration

The minimum inhibitory concentration was determined by using phytopathogenic fungi, which were directly isolated from diseased plants in the National Agricultural Research Institute of Agriculture and Microbiology, using the fuzacidin sample prepared in the same manner as in Example 2-2. Obtained. The minimum inhibitory concentrations are aseptic in the sample of fuzacidin in various concentrations (1.0, 2.0, 4.0, 8.0, 16.0, 32.0, 64.0, 128.0, 256.0, and 512.0 ppm) in the fungal medium PDA (Potato Dextrose Agar, Difco). After adding to the Petri dish (87.5mm) to prepare a solid medium, and then the mycelia of the pathogens were cut with a cork of 5mm size, placed at regular intervals on a solid medium and incubated for 3 days in a thermostat at 25 ℃ growth inhibition effect The minimum inhibitory concentration was determined by observing.

As a result, the result of Table 4 was obtained. Fuzacidin of the present invention exhibited relatively good anti-bioactivity against phytophthora capsaicin and Pythum ultimum . In particular, fuzacidin was confirmed that the antibiotic effect on the plant pathogens at 16 ppm or more confirmed that the plant disease inhibitory effect confirmed in Examples 2-1 to 2-4 was not due to the antibiotic effect. .

De Perez of Dein  main Phytopathogenic bacteria  Minimum inhibitory concentration MIC ) Fungal pathogens MIC (ppm) Raiztonia Solani ( Rhizoctonia solani ) > 512 Fujingum  OxySproom ( Fusarium oxysporum ) 128 In pit photo  Capsai City ( Phytphthora capsici ) 16 Call fp totree dance  Akutatum ( Colletotrichum acutatum ) 256 Pitium  Ultimate ( Pythium ultimum ) 16 Alternaria  Alternate ( Alternaria alternate ) 256 Portree teeth Cine Leah (Botrytis cinerea ) 32 Sellotininia  Celerio Tio Room Sclerotinia sclerotiorum ) 32

As shown in the various examples described above, a sample prepared by diluting fuzacidin isolated and purified from a culture medium of F. Bacillus polymix Inc. E681 to a low concentration (0.01 to 1 ppm in Example 1, Example 2 When 0.1 ~ 1 ppm concentration) was pretreated to red pepper and cucumber seedlings by irrigation, foliar spraying and foliar injection, it showed excellent control effect against red pepper bacterial spot pattern disease, red pepper blight and cucumber urine disease. Appeared to be done.

Through the above results, it was confirmed that fuzacidin of the present invention exhibited excellent plant disease control effects through resistance induction mechanisms in addition to the functions of antibiotics known in the prior art, even when treated with plant useful microorganisms and culture medium of microorganisms. It can be seen that the effect can be expected.

In the present invention, a purified sample was prepared and used for assaying the useful effect of fuzacidin, but in actual agricultural use, partially purified fuzacidine, such as F.C. Could be.

Claims (13)

delete delete delete Consisting of pepper blight, pepper bacterial spot pattern disease, and cucumber beetle containing fusaricidin as an active ingredient, including LI-F04a, LI-F04b, LI-F05a, LI-F05b, LI-F08a, and LI-F08b Plant disease resistance inducer selected from the group.
delete The resistance inducing agent according to claim 4, wherein the fuzacidine is contained in an amount of 10 ng / ml (0.01 ppm) to 1 µg / ml (1 ppm).
delete delete delete delete Capsicum blight, comprising treating fusicidin comprising LI-F04a, LI-F04b, LI-F05a, LI-F05b, LI-F08a and LI-F08b to pepper or cucumber, its seeds, or its habitat A method of inducing resistance to plant diseases selected from the group consisting of red pepper bacterial spot pattern disease and cucumber radish disease.
delete 12. The method of claim 11, wherein the fuzacidine is treated at 10 ng / ml (0.01 ppm) to 1 µg / ml (1 ppm).

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