KR20170054894A

KR20170054894A - Composition for controlling root knot nematode comprising kojic acid as effective component and uses thereof

Info

Publication number: KR20170054894A
Application number: KR1020150157609A
Authority: KR
Inventors: 김진철; 장자영; 김태윤; 이향범; 전선정; 이혜원; 김지형; 배창환; 여주홍
Original assignee: 전남대학교산학협력단; 대한민국(환경부 국립생물자원관장)
Priority date: 2015-11-10
Filing date: 2015-11-10
Publication date: 2017-05-18
Also published as: KR101802911B1

Abstract

The present invention relates to a composition for controlling root knot nematode comprising kojic acid as an active ingredient and use thereof. More specifically, an active material having an effect of killing root knot nematode (Meloidogynesp.) parasitic on crops growing on soil was isolated from a strain of Aspergillus oryzae, and the active material was identified to be kojic acid through analysis of EI-MS, ^1H- and ^1^3C-NMR. Accordingly, kojic acid, isolated from the strain of Aspergillus oryzae, exhibits an effect of inhibiting hatching of larva and eggs of root knot nematode. Therefore, the composition can be used as an eco-friendly pesticide by utilizing an advantage that the composition of the present invention can be obtained from a natural material when the composition is used.

Description

[0001] The present invention relates to a composition for controlling root-knot nematodes containing kojic acid as an active ingredient and a use thereof.

The present invention relates to a composition for controlling root-knot nematodes containing kojic acid as an active ingredient, and a use thereof, and more particularly to a root-knot nematode control agent containing kojic acid or an agriculturally acceptable salt thereof as an active ingredient A method for controlling root-knot nematism comprising treating a plant part, soil or seed with an effective amount of a composition for controlling root-knot nematia, and a method for controlling kojic acid or an agriculturally acceptable salt thereof as an active ingredient To a composition for preventing or treating infections of root-knot nematodes.

The nematode belonging to Platyhelminthes is a small soil animal with a size of 400 ~ 2,000 ㎛. It is symmetrical in shape and spindle shape and has hard skin. Nematodes generally have a population of about 750,000 / m ² in soil. Depending on their prey, they can be divided into bacterial predation, bacterial dispersion, decomposition and production of organic matter, and vegetation formation. In particular, plant nematode nematodes occur in the cultivated soil each year and cause serious damage. Among them, Meloidogyne sp. Is the most economically important plant parasitic nematode. It is recorded as a host plant of about 1,700 kinds of plants. It has high climate adaptability and is distributed worldwide. In Korea, M. incognita , M. javanica , M. arenaria , M. cruciani , M. hapla , and the like, Damage caused by root - knot nematodes and damages are gradually increasing.

As a method of controlling root-knot nematodes, there are physical control methods such as desalination using water and solar heat, rotation, rotation, paddy-upland rotation, and chemical control methods using chemical nematicide. However, in the case of physical control, practical use is limited due to control of cultivation time, manpower, and time constraints. In addition, chemical control methods are excellent in their efficacy and convenience. However, they are problematic in terms of residual problems, And thus the use thereof is limited. Therefore, there is an urgent need to develop a more environmentally and humanly safe and effective nematode that can replace this. As a method for controlling plant-derived nematodes in an environmentally friendly manner, there is a method using microorganisms or metabolites produced by them. Fungi have a variety of metabolic pathways and are capable of synthesizing diverse metabolites, which are known to produce many antibiotics and a variety of commercially available materials.

Korean Patent No. 1501033 discloses a Streptomyces Goyansis strain BIG11003 having a fungicidal effect and its use. In Korean Patent No. 1246707, there is disclosed a strain of Streptomyces Goyansensis Bacterium, Discloses a composition for controlling root-knot nematodes. However, the composition for controlling root-knot nematodes containing kojic acid of the present invention as an active ingredient and its use have not been mentioned yet.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned needs, and it is an object of the present invention to provide a method for screening a substance having a nematicidal activity against a root-knot nematode ( Meloidogyne sp.) Parasitic on a soil crop by using Aspergillus oryzae strains. The strain was grown on a tree ( Cornus alba ) leaves. Ethyl acetate extract was the best in the culture filtrate of the strains. As a result, EI-MS and ¹ H- and ¹³ C-NMR analysis showed that the active substance It was confirmed that it was kojic acid. Thus, the present inventors completed the present invention by confirming that koji acid isolated from Aspergillus oryzae strain exhibits the effect of inhibiting the nematode larva and egg hatching.

In order to accomplish the above object, the present invention provides a composition for controlling root-knot nematitis comprising kojic acid or an agriculturally acceptable salt thereof as an active ingredient.

In addition, the present invention provides a method for controlling root-knot nematia comprising the step of treating an effective amount of the composition for controlling root-knot nematosis to a plant part, soil or seed.

The present invention also provides a composition for preventing or treating infections of root-knot nematodes containing kojic acid or an agriculturally acceptable salt thereof as an active ingredient.

The present invention relates to an active substance having a nematocidal effect against a root-knot nematode ( Meloidogyne sp.) Parasitic on soil crops by Aspergillus oryzae ). From the EI-MS and ¹ H- and ¹³ C-NMR analyzes, it was confirmed that the active substance was kojic acid. Therefore, koji acid isolated from Aspergillus oryzae strain shows the effect of inhibiting the nematode larvae and egg hatching, so that it can be used as an environment friendly nematicide taking advantage of being obtained from natural products.

FIG. 1 is huinmal without trees (Cornus Aspergillus ( Aspergillus oryzae) isolated from alba leaves oryzae ) NIBRFGC000004108 The morphological (A) and molecular systematic (B) identification results of the strain are shown.
2 is Aspergillus duck material (Aspergillus oryzae ) NIBRFGC000004108 This shows the activity of nematode and inhibition of egg hatching against the second instar larva of M. incognita in culture filtrate.
3 is Aspergillus duck material (Aspergillus oryzae ) NIBRFGC000004108. The results of this study were as follows: 1.
FIG. 4 is a schematic view of an Aspergillus (A) and the maximum absorbance (B) of the nematicide active substance isolated from the strain of oryzae NIBRFGC000004108.
FIG. 5 is a schematic view of an Aspergillus oryzae ) NIBRFGC000004108 This is the nematode activity against the second instar larva of M. incognita of kojic acid isolated from the culture of the culture filtrate. Cinnamaldehyde is a positive control indicating the nematode activity.
FIG. 6 is a photograph of Aspergillus oryzae ) NIBRFGC000004108 The inhibition rate of egg hatching of M. incognita of kojic acid isolated from the extract of the culture filtrate. Cinnamaldehyde is a positive control indicating the nematode activity.

In order to accomplish the present invention, the present invention provides a composition for controlling root-knot nematitis comprising kojic acid or an agriculturally acceptable salt thereof as an active ingredient represented by the following formula (1).

The root-knot nematode controlling composition of the present invention contains kojic acid or an agriculturally acceptable salt thereof as an active ingredient, and may further contain a nematicide active substance. The nematicide active substance may be, but is not limited to, an avermectin type nematicide active substance, morantel tartrate, mesulfenfos, levamisol, and the like.

The avermectin-based nematicide can be, but is not limited to, emamectin benzoate, abamectin, milbemectin, ivermectin, and the like.

In the composition for controlling root-knot nematodes according to the present invention, the nematode activity is generally expressed by the root-knot nematode ( Meloidogyne sp.); Sister nematodes such as Heterodera sp., Globodera sp. And the like; Primateen (Pratylenchus.); Tylenchulus semipenetrans; Radopholus; Ditylenchus; Anguina; It is a live nematode activity against Aphelenchoides, preferably a live nematode activity against the root nematode ( Meloidogyne sp.), But is not limited thereto.

In the composition for controlling root-knot nematodes according to the present invention, the root-knot nematode may be, but is not limited to, Meloidogyne incognita .

In the composition for controlling root-knot nematodes according to the present invention, the composition may inhibit the growth or egg hatching of the root-knot nematode, but is not limited thereto.

The composition for controlling root-knot nematitis according to the present invention may be in the form of, for example, directly sprayable solutions, powders and suspensions, or in the form of highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, But are not limited thereto.

The root-knot nematode controlling composition of the present invention can be formulated into various forms. The preparation can be prepared, for example, by adding a solvent and / or a carrier. Often, inert additives and surface-active materials, such as emulsifiers or dispersants, are mixed into the formulation. Suitable surface-active materials include, but are not limited to, aromatic sulfonic acids (e.g., lignosulfonic acid, phenol-sulfonic acid, naphthalene- and dibutylnaphthalenesulfonic acid), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, A salt of a fatty alcohol glycol ether, a sulfonate naphthalene and derivatives thereof, a condensate of formaldehyde, a condensate of naphthalene or naphthalene sulfonic acid, a phenol and a formaldehyde Condensates, polyoxyethylene octylphenol ethers, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ethers, alkylaryl polyether alcohols, isotridecyl alcohol, fatty alcohol / ethylene oxide condensates , Ethoxylated castor oil, polyoxyethylene alkyl ether or polyoxypropylene, lauryl alcohol Polyglycol ether acetate, sorbitol esters, lignin-sulfite waste liquid, or may be a cellulose, but is not limited thereto.

Suitable solid carrier materials are in principle all porous and comprise an agriculturally acceptable carrier such as mineral earths such as silica, silica gel, silicate, talc, kaolin, limestone, limestone, chalk, Fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, vegetable products such as cereal flour, bark powder, wood meal, And nut shell powder), or cellulose powder. The solid carrier may be used alone or in combination of two or more.

The root-knot nematode controlling composition of the present invention can be used for, but is not limited to, a tube-receiving, a leaf-surface spraying, a seed sterilizing, or an agricultural equipment disinfection.

The root-knot nematode controlling composition of the present invention can be mixed with a dispersing agent, a penetrating agent, or a surfactant in order to improve crop absorption and effect.

The method for controlling the root-knot nematode may be performed by spraying or spraying a composition for controlling root-knot nematode inhibiting composition using an effective amount, but is not limited thereto.

[Chemical Formula 1]

The composition for preventing or treating infections of root-knot nematodes according to the present invention is particularly useful for the prevention of diseases caused by root-knot nematodes, In particular, since interest in pollution-free and safe agricultural products is increasing and demand is increasing, and the awareness of residual toxicity and environmental pollution due to the use of pesticides is rising, pollution-free safety products Which can greatly contribute to the production of

The composition for preventing or treating infection with root-knot nematodes according to the present invention is a composition for preventing or treating nematode infestation, wherein the nematode-damaged cereals such as wheat, barley, rye, oats, rice, (Such as sugar cane and related crops), sugar beets (such as sugar beets and feed beets), cucumber plants (such as oil, squash, melons), vegetables (such as spinach, lettuce, asparagus, cabbage and brassica, Citrus fruit (such as orange, lemon, grapefruit, Chinese mandarin), fruit (such as apple, pear, plum, peach, almond, cherry, strawberry, raspberry and blackberry) (Eg, cotton, flaxseed, poppy, olive, sunflower seed, coconut, castor oil plant, cocoa seed, peanut), alfalfa, , Hemp, jute), Laura (For example, avocado, cinnamon, camphor), deciduous trees and conifers (for example, timber, chestnut, duck, poplar, birch, fir, larch, pine) (For example, but not limited to, coffee, sugarcane, tea, vine, hops, bananas and natural rubber trees) or to the cultivated soil or seeds of these plants. . On the other hand, in the case where it is necessary to prevent or treat nematode infestation in plant parts, soil, seeds, or where soil purification by removal of nematode is necessary, microorganisms having nematicidal activity of the present invention and / It may be possible to suppress the damage caused by nematodes by administering both microorganisms and chemical insecticides simultaneously.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are merely illustrative of the present invention and that the scope of the present invention is not limited thereto.

Example One. Aspergillus Duck Aspergillus oryzae ) NIBRFGC000004108 Morphological and Molecular systematics Classification

Trees with white horses ( Cornus alba) by Aspergillus duck isolated from leaf material (Aspergillus The morphology of the strains (microstructure such as mycelia, spores, fruiting body, and ultrafine structure by SEM) was firstly identified. Subsequently, molecular classifications were performed to identify species levels. The isolated microorganisms were grown on a cellophane-coated PDA medium, and then the genomic DNA (gDNA) was extracted from the microbial cells to obtain an internal transcribed spacer rDNA region (SEQ ID NO: 1), CaM (calmodulin, 2) and BenA (β-tubulin, SEQ ID NO: 3) genes, which were then purified and sequenced. For the ITS rDNA region, CaM and BenA, each of the primers disclosed in Table 1 below was used. In order to construct a phylogenetic tree, a molecular phylogenetic analysis based on a complex data set gene sequence was carried out. As a result of morphological observation and molecular genetic analysis based on the gene sequence, as shown in Fig. 1, Aspergillus oryzae ) NIBRFGC000004108 strain is Aspergillus oryzae ), and the strain thus isolated was identified as Aspergillus oryzae ) NIBRFGC000004108 strain, deposited at the Institute of Agricultural Biotechnology on Oct. 28, 2015 (Accession No .: KACC83002BP).

The primers used in the present invention Target gene Name of the primer SEQ ID NO: Base sequence ITS rDNA ITS 4 TTA CGT CCC TGC CCT TTG TA ITS4 5 TCC TCC GCT TAT TGA TAT GC CaM Cmd5 6 CCG AGT ACA AGG ARG CCT TC Cmd6 7 CCG ATR GGT GTC ATR ACG TGG BenA Bt2a 8 GGT AAC CAA ATC GGT GCT GCT TTC Bt2b 9 ACC CTC AGT GTA GTG ACC CTT GGC

Example 2. Aspergillus Duck Aspergillus oryzae ) NIBRFGC000004108 Of culture filtrate Live nematode activity And egg hatching inhibitory activity

Aspergillus oryzae ) NIBRFGC000004108 strain was cultured in PDA (potato dextrose agar) medium at 25 ° C for 7 days. Aspergillus Aspergillus oryzae ) NIBRFGC000004108 From the edge of the strain, 5 pieces of mycelia were detached using a cork board having a diameter of 8 mm, inoculated into a PDB (potato dextrose broth) medium, and cultured at 150 rpm and 25 ° C for 14 days with shaking. After 14 days, the cells were removed using 4-layer gauze, and only the culture filtrate was obtained to examine the nematode activity. In order to obtain root nodule nematodes from tomato roots infested with root nematodes, the roots of the plants were washed with tap water to remove foreign matter, and the roots were cut into 1 cm size, and 0.5% sodium hypochlorite solution was added thereto. Lt; / RTI > After crushing, 45 mu m sieve and 25 mu m sieve were sandwiched and the eggs were separated. The separated eggs were used for the present example by isolating the hatched larvae using a baermann funnel.

Fifty livers of the root-knot nematode and 150 egg suspensions were placed in each well of a 96-well microplate, and 5, 10 and 20% Aspergillus oryzae NIBRFGC000004108 culture filtrate (hereinafter referred to as "sample" ) Was treated to make a final volume of 100 μl per well. After the sample treatment, the 96-well plate was agitated for 30 seconds so that the samples were well mixed, and then stored in a plastic bottle having a relative humidity of 100% at room temperature. 72 hours after the sample treatment, the mortality and the egg hatching inhibition rate were examined under an optical inverted microscope. The live nematode rate was determined as a living nematode by determining the nematode as a nematode that died straight from a nematode that had no movement and was flexible and curved. The nematode rate was examined using the following equation.

(%) = [(Lethal rate of treatment - lethal rate of control) / (100 - lethal rate of control)] × 100

(%) = [(Egg hatching rate of control - egg hatching rate of treatment) / egg hatching rate of control] x 100

Egg hatching rate (%) = [number of larvae / (number of eggs + number of larvae)] × 100

As a result, when the culture filtrate of 5, 10 and 20% was treated as shown in Fig. 2, the inhibitory activity of egg hatching was 2.5, 14.8 and 76.9% and 47.8, 57.7 and 80.0% Dependent nematode activity.

Example 3. Aspergillus Duck Aspergillus oryzae ) NIBRFGC000004108 Of culture filtrate By solvent fraction Live nematode activity

Aspergillus oryzae ) NIBRFGC000004108 Culturing of the strains 700 ml of the filtrate was subjected to sequential two-fold extraction with the same amount of ethyl acetate and 70% (v / v) butanol. The three fraction layers obtained by solvent fractionation were concentrated under reduced pressure to obtain 597 mg of the ethyl acetate layer, 1,326 mg of the butanol layer and 4,283 mg of the water layer. The nematode activity of the nematode larvae was examined. Prepared Aspergillus oryzae ) The ethyl acetate extract of NIBRFGC000004108 was dissolved in acetone, the butanol extract was dissolved in methanol and the water layer was dissolved in water to 100 times higher concentration, respectively, and then treated with nematode suspension at 50, 100 and 200 μg / ml, respectively. The control was treated with 1% acetone or methanol. The 96-well plate was agitated for 30 seconds to mix well, placed in a plastic bottle with a relative humidity of 100%, and stored at room temperature. After 72 hours of the sample treatment, the mortality was examined by the method described above.

As a result, as shown in FIG. 3, the ethyl acetate layer exhibited 65.9% and 74.9% high pesticidal activity at 100 and 200 μg / ml, and the butanol layer and the water layer did not show any activity at all. Therefore, separation of substances showing carnivorous activity was carried out using the ethyl acetate layer having the highest activity in Example 4 below.

Example 4. Live nematode activity Material separation

The ethyl acetate extract with high nematicidal activity was fractionated by TLC (Kiesel gel 60GF 254, 0.5 mm film thickness, E. Merck) using chloroform: methanol (7: 1, v / v) Were scraped and extracted with acetone and methanol according to Rf value, and finally 7 fractions (PF1 to PF7) were obtained. The 7 fractions obtained showed the best activity in the PF5 fraction (Rf value: 0.19). HPLC analysis was performed to investigate whether the PF5 fraction was pure substance . The column was washed with C18 reverse phase column (Atlantis T3, 5 m, 4.6 250 mm, Waters, Co., Ireland) using methanol containing 0.1% acetic acid and methanol containing 0.1% acetic acid for 30 minutes While increasing the specific gravity of organic solvent from 10% to 50% of methanol.

As a result, it was confirmed that one chromatogram was present in the 7th minute as shown in Fig. 4, which showed the maximum absorbance at 216.0 nm and 268.8 nm. Finally, the PF5 (7 mg) material was purely isolated.

Example 5. Determine the structure of the separated material

The obtained PF5 material was identified by EI-MS and ¹ H- and ¹³ C-NMR. As a result of the EI-MS analysis, m / z 142 was detected as the [M] ⁺ value of the nematicide active substance, and it was confirmed through MS and NMR analysis that it has the molecular formula of C ₆ H ₆ O ₄ . ¹ H- and < ¹³ > C-NMR data of the separated active substance are as shown in Table 2 below. In the ¹ H-NMR spectrum of the nematode material, a methine proton signal attributed to the 4-pyrone structure was observed at 7.95 ppm and 6.51 ppm, and a methine proton signal attributable to the hydroxyl group at 4.42 ppm A methylene proton signal was observed. Six carbon signals were observed in the ¹³ C-NMR spectrum and four signals participating in the pyrone group were observed at 110.84, 141.12, 147.71 and 170.46 ppm, and the carbonyl of the pyrone group The signal due to carbonyl was observed at 177.16 ppm and the signal of methylene carbon with alcohol was observed at 61.33 ppm. Using such spectroscopic data, Aspergillus oryzae NIBRFGC000004108 The nematicide active material produced by the strain is 5-hydroxy-2-hydroxymethyl-4H-pyran-4-one, (kojic acid).

¹ H- and ¹³ C-NMR data of isolated kojic acid Position Kojic acid δ _C [delta] _H (mult, J , Hz) One 147.71, CH 7.95, s 2 170.46, C - 3 177.16, C - 4 141.12, CH 6.51, s 5 110.84, C - 6 61.33, CH ₂ 4.42, s 2C-OH - - 6C-OH - -

Example 6. The separated active substance Kojic acid Root-knot nematode Second generation Larval and egg hatching inhibitory activity

In order to investigate the killing activity and the inhibition of egg hatching of kojiic acid as a separated active substance, Aspergillus oryzae ) NIBRFGC000004108 culture solution (hereinafter referred to as "sample") was dissolved in dimethyl sulfoxide (DMSO), and cinnamaldehyde having nematode activity was dissolved in ethanol and used as a positive control in this example. All samples were prepared at a concentration 100 times higher than the treatment concentration and examined for carcinogen activity at 4, 12, 37, 111, 333 and 1,000 ㎍ / ㎖ levels. As a negative control, 1% DMSO and ethanol were used, and the activity of nematode and egg hatching inhibition was examined by the method described in Example 1 above. The experiment was repeated 3 times, and the live nematode rate was examined 3 days after the sample treatment, and the inhibition rate of egg hatching was examined 7 days later.

As a result, the live nematode activity of kojiic acid, an active substance, and the inhibition rate of egg hatching increased in a concentration dependent manner, and the live nematode activity against the second instar larva of the root nodule nematode reached to 1,000 .mu.g / Ml and 90% (FIG. 5). LC ₅₀ The results are shown in Table 3 below. In addition, the inhibition rates of egg hatching were respectively 90.9% and 96.4% at 333 and 1,000 / / ㎖, respectively, which exhibited excellent nematicidal activity, which was similar to that of Shin-Nam aldehyde treated as a positive control (Fig. 6) It was confirmed that the egg hatching inhibitory activity was superior to the live nematode activity.

Aspergillus oryzae ) NIBRFGC000004108 LC ₅₀ value of koji acid which is a nematode substance isolated from strain and M. incognita of cinnamaldehyde which is positive control group sample LC ₅₀ ([mu] g / ml) 72 h R ² Kojic acid 118.1 0.96 Shin Nam Aldehyde 3.5 0.97

<110> INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY National Institute of Biological Resources <120> Composition for controlling root knot nematode comprising kojic acid as an effective component and uses thereof <130> PN15304 <160> 9 <170> Kopatentin 2.0 <210> 1 <211> 664 <212> DNA <213> Aspergillus oryzae <400> 1 acctaggccg gagggacgca ttcatgcagt cggaggagct attcgtaaag cggaaggatc 60 atatcaataa gcggaggagc atatcaagga gcaggaggat catatcgaga acgcaggacg 120 atcacctcga gaaacaccac gatcactgta tgatctcgtg aattctcagt tgatcgtatc 180 gaaaaccgtt gaatctttcg aagaagcatc tcttggttcc gagcatcgat gaaaaacgca 240 gcgaaatgcg ataactagtg tgaattgcag aattccgtga atcatcgagt ctttgaacgc 300 acattgcgcc ccctggtatt ccggggggca tgcctgtccg agcgtcattg ctgcccatca 360 agcacggctt gtgtgttggg tcgtcgtccc ctctccgggg gggacgggcc ccaaaggcag 420 cggcggcacc gcgtccgatc ctcgagcgta tggggctttg tcacccgctc tgtaggcccg 480 gccggcgctt gccgaacgca aatcaatctt tttccaggtt gacctcggat caggtaggga 540 tacccgctga acttaagcat atcataagcc ggaggaaaca tatctacaga gcggagggct 600 ccttacgtgc ccgcgccccc ttgtttacgg tactaaatgg ggggaaaaaa attgaatttg 660 gaag 664 <210> 2 <211> 582 <212> DNA <213> Aspergillus oryzae <400> 2 cattaagctg ctatcgtcgt tcgtgaaatt ggttttgtta gtcgtcatga tttgaacaca 60 agctgacttg gcttttcttg ggtttcctat aggacaagga cggtgatggt tagtacagtt 120 tattttattc attctccctt caaatgcgat caatatgttt tagccgccat aattttatcc 180 agtttctgtt cgatcggctg aagtcttggc attgatgaat tgacttgata tgcaggccag 240 atcaccacca aggagttggg cactgtcatg cgctctctgg gccaaaaccc ctctgagtcg 300 gaactccagg acatgattaa cgaggttgac gccgacaaca atggcaccat tgacttccct 360 ggtacgagac ggcttccgta cgattcataa atgaaatagc tgttaatgtt caaatagagt 420 tccttacgat gatggcgaga aagatgaagg ataccgactc tgaggaggag atccgggagg 480 ctttcaaggt tttcgaccgc gataacaacg gcttcatctc cgctgccgaa ttgcgccacg 540 tagccccccc ccctgggggg aaaggccagg cggggggtga ag 582 <210> 3 <211> 500 <212> DNA <213> Aspergillus oryzae <400> 3 ggggggtcta tgccnttcga gttagtatgc tttggacaag gaactcctca aaagcatgat 60 ctcggatgtg tcctgttata tctgccacat gtttgctaac aactttgcag gcaaaccatc 120 tctggcgagc acggccttga cggctccggt gtgtaagtac agcctgtata cacctcgaac 180 gaacgacgac catatggcat tagaagttgg aatggatctg acggcaagga tagttacaat 240 ggctcctccg atctccagct ggagcgtatg aacgtctact tcaacgaggt gcgtacctca 300 aaatttcagc atctatgaaa acgctttgca actcctgacc gcttctccag gccagcggaa 360 acaagtatgt ccctcgtgcc gtcctcgttg atcttgagcc tggtaccatg gacgccgtcc 420 gtgccggtcc cttcggtcag ctcttccgtc ccgacaactt cgttttcggc cagtccggtg 480 ctggtaacaa ctgggccaag 500 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 ttacgtccct gccctttgta 20 <210> 5 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 5 tcctccgctt attgatatgc 20 <210> 6 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 6 ccgagtacaa ggargccttc 20 <210> 7 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 7 ccgatrgagg tcatracgtg g 21 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 8 ggtaaccaaa tcggtgctgc tttc 24 <210> 9 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 9 accctcagtg tagtgaccct tggc 24

Claims

1. A composition for controlling root-knot nematitis comprising kojic acid or an agriculturally acceptable salt thereof as an active ingredient represented by the following formula (1).
[Chemical Formula 1]

The composition according to claim 1, further comprising a nematicide active substance.

3. The method of claim 2, wherein the nematicidal active substance is selected from the group consisting of avermectin based nematicidal active substances, morantel tartrate, mesulfenfos, and levamisol Wherein the composition is at least one compound selected from the group consisting of compounds of formula (I).

4. The method according to claim 3, wherein the avermectin-based nematicide is emamectin benzoate, abamectin, milbemectin or ivermectin. A composition for nematode control.

The composition according to claim 1, wherein the root nematode is Meloidogyne incognita .

[3] The composition according to claim 1, wherein the composition inhibits the growth or egg hatching of root-knot nematodes.

A method for controlling a root-knot nematode comprising the step of treating an effective amount of a composition for controlling root-knot nematode according to any one of claims 1 to 6 to a plant part, soil or seed.

A composition for preventing or treating infections of root-knot nematodes comprising kojic acid or an agriculturally acceptable salt thereof as an active ingredient represented by the following formula (1).
[Chemical Formula 1]