KR20170022750A

KR20170022750A - Hydrogel alginate non-woven mask pack using alginate lyase

Info

Publication number: KR20170022750A
Application number: KR1020150118150A
Authority: KR
Inventors: 김임준
Original assignee: (주)진코스텍
Priority date: 2015-08-21
Filing date: 2015-08-21
Publication date: 2017-03-02
Also published as: KR101746196B1

Abstract

An alginate nonwoven mask pack prepared by mixing calcium alginate polymer fibers and different types of fibers by using alginate decomposing enzyme, wherein the alginate nonwoven mask pack comprises an insoluble calcium alginate polymer in the calcium alginate polymer fiber, And is modified with water-soluble oligomer alginate by using an alginate degrading enzyme.

Description

(Hydrogel alginate non-woven mask pack using alginate lyase}

An alginate nonwoven mask pack prepared by mixing calcium alginate polymer fibers and different types of fibers by using alginate decomposing enzyme, wherein the alginate nonwoven mask pack comprises an insoluble calcium alginate polymer in the calcium alginate polymer fiber, Wherein the alginate degrading enzyme is modified with a water-soluble oligomer alginate.

In general, the cosmetic mask pack is made by adding water or lotion to a sheet such as a nonwoven fabric, or adding a cosmetic solution such as a natural or synthetic substance known to have a function. Such a cosmetic mask pack is a functional cosmetic product which is attached to the face and supplies the cosmetic ingredient contained in the mask pack to the skin, thereby moisturizing the skin and removing the waste matter of the keratin and pore to restore the skin physiological function It is a kind.

In such a mask pack, there are various kinds of sheets to which a cosmetic ingredient is applied, and among them, a nonwoven fabric type which can be made thin is widely used. The nonwoven mask pack is manufactured by cutting a thin nonwoven fabric into a face shape and immersing the cosmetic ingredient on one face thereof, which is advantageous in that it is inexpensive and easy to use.

However, even if the nonwoven mask pack is fixed to the skin, the nonwoven mask pack is not firmly adhered to a desired site, and a part of the pack is excited at the bending portion, so that the cosmetic ingredient of the mask pack is not effectively transferred to the skin. In addition, the synthetic polymer is often added to the nonwoven fabric in accordance with the manufacturing process, and when the cosmetic liquid is added, the resultant fiber becomes stiff, and the feeling of use is poor and the skin affinity is low. In addition, in the case of a formulation in which the nonwoven fabric is soaked in an additive such as a cosmetic liquid to induce continuous absorption of the cosmetic liquid, the skin adhesion is poor, and since too high a cosmetic ingredient comes into contact with the skin at an early stage, disadvantage And 10 to 15 minutes, the moisture is evaporated and dried, and the mask pack falls off the face.

In order to overcome the drawbacks of such a nonwoven mask pack, research and development on a hydrogel mask pack have been actively conducted. The hydrogel is a material having a three-dimensional hydrophilic polymer network structure using purified water as a dispersion medium. The hydrogel is capable of absorbing a large amount of water, and has the same flexibility as a natural tissue. The hydrogel has elasticity, skin adhesion, Is used as a mask pack substrate improved in moisturizing, nutritional supply or exfoliation effect.

However, when a mask pack is manufactured using only the hydrogel itself, the shape of the mask pack may be difficult to maintain due to its low mechanical strength. In order to maintain the shape of the mask pack, a synthetic resin, a nonwoven fabric, ), And the like. However, in this case, since the gel-like hydrogel may elute the chemical components of the synthetic resin, it is necessary to examine from the standpoint of biocompatibility and stability, as well as inconvenience due to the use of the support, insufficient cosmetic ingredients and relatively high There are disadvantages such as price.

In order to improve the disadvantages of the nonwoven mask pack and the hydrogel mask pack as described above, a hydrogel mask pack, a method for producing the same, and a composition used for the production thereof (hereinafter referred to as Patent Document 1 Discloses a hydrogel nonwoven fabric, a method for producing the nonwoven fabric, and a mask pack (hereinafter referred to as "Patent Document 2") using the same, which are disclosed in Japanese Patent Application Laid-Open No. 10-1296105.

However, in Patent Document 1, a hydrogel mask pack is manufactured by gelating a composition for a hydrogel mask pack. Rather, due to excessive adhesive force of the product itself, a phenomenon of aggregation occurs in the mask pack product itself, There is an inconvenience. Patent Document 2 discloses that a hydrogel is impregnated using a nonwoven fabric as a mask pack support, and there is a possibility that the gel-like hydrogel still elutes the chemical components of the synthetic resin, thereby causing skin trouble due to skin irritation There was a problem that could be.

Patent Document 1: Korean Patent Registration No. 10-0841742 Patent Document 2: Korean Patent Registration No. 10-1296105

SUMMARY OF THE INVENTION The object of the present invention is to solve the above-mentioned problems, and it is an object of the present invention to provide a water-soluble oligomer alginate modified with water-soluble oligomer alginate by using an alginate degrading enzyme in an insoluble calcium alginate polymer in calcium alginate polymer fiber, A hydrogeled alginate nonwoven mask pack using an alginate degrading enzyme having an anti-inflammatory effect.

In addition, by minimizing evaporation of the hydrogel cosmetic liquid through formation of micro-film of water-soluble alginate component and high water retention ability, it is possible to supply sufficient nutrition to the skin and to absorb and remove heavy metals of the skin, to inhibit harmful substances, And a hydrogel alginate nonwoven mask pack using alginate degrading enzyme having excellent bioactivity.

In order to solve the above-mentioned problems, the hydrogel alginate nonwoven mask pack using alginate decomposing enzyme according to the present invention is an alginate nonwoven mask pack prepared by blending calcium alginate polymer fibers and different types of fibers, wherein the calcium alginate polymer fibers Characterized in that the insoluble calcium alginate polymer is modified with a water-soluble oligomer alginate using an alginate decomposing enzyme.

The alginate decomposing enzyme is included in the essence liquid impregnated in the alginate nonwoven mask pack.

The modification may be performed by reacting the insoluble calcium alginate polymer with poly (M) lyase [EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] or poly (G) lyase [EC 4.2.2.11 (1 → 4) L-guluronan lyase] in the presence of an alginate degrading enzyme.

The alginate degrading enzyme contained in the essence solution may be selected from the group consisting of the (M) lyase (EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] or the poly (G) lyase [EC 4.2.2.11 -α-L-guluronan lyase], or mixed in a predetermined ratio, wherein the essence solution impregnated in the nonwoven mask pack contains 0.001 ppm to 20,000 ppm of the alginate degrading enzyme .

In addition, the heterogeneous fibers include at least one selected from the group consisting of regenerated fibers and natural fibers.

As described above, according to the present invention, an insoluble calcium alginate polymer in a calcium alginate polymer fiber is modified with a water-soluble oligomer alginate by using an alginate degrading enzyme to provide an anti-whitening, moisturizing, .

In addition, by minimizing evaporation of the hydrogel cosmetic liquid through formation of micro-film of water-soluble alginate component and high water retention ability, it is possible to supply sufficient nutrition to the skin and to absorb and remove heavy metals of the skin, to inhibit harmful substances, And has excellent bioactivity.

Hereinafter, the present invention will be described in detail.

An alginate nonwoven mask pack produced by blending calcium alginate polymer fibers and different types of fibers by using calcium alginate polymer fibers made of alginate as a raw material, Characterized in that the calcium alginate polymer is modified with a water-soluble oligomer alginate using an alginate degrading enzyme.

The alginate of the calcium alginate polymer fiber is a kind of seaweed polysaccharide which constitutes a cell membrane and intercellular substance of brown algae which is one of the marine organisms. It is called alginic acid and is a block of malonic acid (M) unit, a block of glutaric acid (G) And the block of MG unit in the middle is a linear copolymer composed of 1,4-glycoside.

The physicochemical properties of the alginic acid are determined by viscosity, solubility, ion exchange capacity, and the like depending on the relative ratio of the block of malonic acid (M) unit, the block of gluronic acid (G) And its structure is shown in the following reference drawing 1.

[Reference drawing 1]

Structure of Alginate

These alginates are used in the form of sodium alginate, which is water-soluble rather than alginic acid itself, which is usually insoluble in water, because the alginic acid has a carboxyl group (-COOH) of uronic acid in the molecule. When the calcium chloride solution is spun in a coagulating bath, . Particularly, alginic acid has functions and effects such as water retention, stickiness, lubricity, gelation and emulsification stability, physiological activity, and is not toxic to human body, easy to process, Is widely used.

In general, the calcium alginate polymer fiber of the alginate nonwoven mask pack forms calcium insoluble calcium alginate that does not dissolve in water through strong ionic bonding instead of hydrogen of the carboxyl group of the alginate. The skin contact feeling and the feeling of use are lowered, and the cosmetic ingredient of the essence liquid is not effectively transferred to the facial skin.

Therefore, it is necessary to convert the calcium alginate polymer fibers of the nonwoven mask pack into water-soluble alginate fibers so that the adhesion, feel and transparency of the mask pack is excellent. This is because water-soluble calcium alginate polymers in the calcium alginate polymer fibers Thereby making the calcium alginate polymer fibers have water-solubility characteristics.

The modification is carried out using an alginate degrading enzyme. The insoluble calcium alginate polymer is reacted with poly (M) lyase (EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] or poly (G) lyase [EC 4.2.2.11 1? 4) -α-L-guluronan lyase], which is obtained by digesting an alginate-degrading enzyme with water-soluble oligomer alginate.

Wherein the alginate degrading enzyme is selected from the group consisting of the poly (M) lyase [EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] or the poly (G) lyase [EC 4.2.2.11 (1 → 4) The reason for using lyase is that poly (M) lyase [EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] degrades many alginates with mannuronate and poly (G) lyase [EC 4.2.2.11 ) -α-L-guluronan lyase] decomposes insoluble calcium alginate polymers into water-soluble oligomer alginates because guluronate decomposes many alginates.

That is, by using the alginate degrading enzyme, the calcium alginate polymer bond is broken and converted into an oligomer alginate form so that it can be partially or wholly converted into a water-soluble alginate fiber to provide anti-whitening, moisturizing, skin barrier enhancement and anti-inflammation.

The present invention is configured such that the alginate decomposing enzyme is added to an essence liquid impregnated in an alginate nonwoven mask pack prepared by mixing calcium alginate polymer fibers and different fibers in a sheet form.

Therefore, it is not necessary to carry out a separate process to convert the insoluble calcium alginate polymer fiber into the water-soluble alginate oligomer fiber, and it is possible to effectively reduce the mask pack manufacturing process and manufacturing cost.

Meanwhile, the alginate degrading enzyme added to the essence solution is the poly (M) lyase [EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] or the poly (G) lyase [EC 4.2.2.11 ) -α-L-guluronan lyase] or a mixture thereof in a predetermined ratio, and the essence liquid preferably contains 0.001 ppm to 20,000 ppm of the alginate degrading enzyme.

The reason for this is that when the alginate degrading enzyme is added to the essence solution at a rate of 0.001 ppm or less, the decomposition activity is not properly expressed, so that the calcium alginate polymer bond may not be decomposed, and sufficient addition of the alginate degrading enzyme at 20,000 ppm This is because the decomposition effect can be obtained.

As described above, the present invention minimizes the evaporation of the hydrogel cosmetic liquid through the formation of the micro-film of the water-soluble alginate component and the high moisture retaining ability, thereby providing sufficient nutrients to the skin and absorbing and removing heavy metals from the skin, It is possible to have toxicity suppression, wound protection, and antibacterial properties.

Meanwhile, in the present invention, the alginate nonwoven mask pack is prepared in a sheet form by blending calcium alginate polymer fibers and different types of fibers, and the different types of fibers include at least one kind selected from the group consisting of regenerated fibers and natural fibers to be.

In the alginate nonwoven mask pack, 10 to 99% by weight of heterogeneous fibers are preferably added, and 10 to 90% by weight of heterogeneous fibers are most preferably added.

The above different kind of fibers are composed of regenerated fibers such as cellulose fibers and rayon fibers, and natural fibers such as cotton fibers, hemp fibers, silk fibers and bamboo fibers instead of the fibers made of synthetic resin, so that not only the feeling of use of the mask pack is excellent but also the calcium alginate polymer fibers The water-soluble hydrogel alginate modified from the insoluble calcium alginate polymer can prevent the chemical components of the synthetic resin from being eluted, thereby minimizing troubles due to skin irritation.

Hereinafter, the present invention will be described in more detail with reference to the following Examples, Comparative Examples and Experimental Examples.

It should be understood, however, that the invention is not construed as being limited to the embodiments set forth herein, but is capable of modifications and equivalents within the spirit and scope of the invention. Will be apparent to those skilled in the art to which the present invention pertains.

[Example 1]

Calcium alginate polymer fibers and cellulose fibers were blended in a weight ratio of 50:50 to prepare an alginate nonwoven mask pack in sheet form.

An essence solution impregnated in the alginate nonwoven mask pack was prepared by adding alginate decomposing enzyme having a component ratio of 0.0001 in order to make cosmetic ingredients in the same proportions as shown in Table 1 below.

Essence liquid ingredient ratio ingredient Composition ratio Purified water 100 Allantoin 0.1 Disodium iodide 0.03 Panthenol 0.3 Adenosine 0.042 El arginine 0.14 1,3-butylene glycol 3.0 glycerin 5.0 Xanthan gum 0.050 Piggy-60 Hydrogenated Castor Oil 0.4 Carbomer 0.1 Hydroxyethylcellulose 0.1 Alginate degrading enzyme 0.0001

Next, an essence liquid prepared as described above was impregnated into the nonwoven mask pack to prepare a hydrogel nonwoven fabric mask pack.

[Example 2]

A hydrogeled alginate nonwoven mask pack was prepared in the same manner as in Example 1, except that the ingredient ratio of alginate degrading enzyme added to the essence solution was 0.1.

[Comparative Example]

In Example 1, a hydrogeled alginate nonwoven mask pack was prepared in the same manner as in Example 1, except that no alginate degrading enzyme was added to the essence solution.

[Experimental Example 1] Whitening effect

The following experiment was conducted to examine the whitening effect on human skin.

First, in 12 healthy men, an opaque tape with a hole of 1.5 cm in diameter was attached to the upper part of the subject's neck, and then the skin was blackened by irradiating the subject with 1.5 to 2 times of the minimum erythema Respectively.

After irradiating the ultraviolet rays, the mask packs prepared in the above Examples and Comparative Examples were stuck for 15 minutes and then peeled off. Thereafter, the state changes were observed for 8 weeks, and the skin color was measured with a colorimeter CR2002 (Minolta Co., Japan) every 1 week.

Then, the difference (ΔL *) between the skin color and the skin color at the time of attaching and removing the mask pack prepared in the above Examples and Comparative Examples was calculated according to the following Equation 1, and the results are shown in Table 2 below. On the other hand, the whitening effect was determined by comparison of? L * between the mask pack attachment sites of Examples 1 and 2 and Comparative Example. When? L * value is about 2, whitening of the deposited pigment is noticeable. As shown in FIG.

The whitening effect (? L *) result division DELTA L * Example 1 1.56 ± 0.21 Example 2 1.80 ± 0.15 Comparative Example 0.56 + 0.11

As a result of the above Table 2, it can be confirmed that the whitening effect of Example 2 in which the essence solution containing the alginate degrading enzyme having the ingredient ratio of 0.1 was impregnated into the nonwoven mask pack exhibited the highest whitening effect. In Example 2, It can be confirmed that it has an excellent whitening effect.

It can be seen that it is possible to improve the whitening effect by modifying the insoluble calcium alginate polymer with an oligomer with water-soluble alginate.

[Experimental Example 2] Effect of moisturizing and skin barrier function improvement

30 healthy women were divided into three groups (A, B, and C) at a temperature of 25 ° C and a relative humidity of 45% in a room without air flow. The mask pack of Example 2 was applied to the group B, and the mask pack of the comparative example was applied to the upper arm of the subjects for the group C three times a day for 6 weeks, and then the TEWAMETER TM300 (C + K electronic Transepidermal Water Loss (TEWL) value was measured by the TEWL value change amount TEWL with time according to the change of the transdermal water loss by using a CORNEOMETER CM 820 PC (C + K electronic GmbH, Germany) The moisture content of the skin was quantified from 0 to 150 according to the change of the electric conductivity according to the moisture content of the skin. The results are shown in Table 3 below.

Moisturizing and skin barrier function improvement result ΔTEWL Corneometer value Example 1 -1.2 122 Example 2 -5.4 131 Example 3 2.1 95

As can be seen from the results shown in Table 3, the hydrogelised alginate nonwoven mask pack modified with the alginate degrading enzyme of the present invention has a much higher transdermal water loss than the unmodified alginate nonwoven mask pack, . Also, it can be seen that Examples 1 and 2 show a higher increase in the corneometer value, which is a measure of the water content of the skin, as compared with the comparative example.

That is, the hydrogel alginate nonwoven mask pack according to the present invention can effectively transfer the cosmetic ingredient of the essence liquid to the skin by modifying the insoluble calcium alginate polymer with the water-soluble alginate monomer, It can be seen that the effect of improving the moisturizing and skin barrier function is improved by improving the transdermal water loss.

[Experimental Example 3] Anti-inflammatory effect

First, 30 women were divided into 3 groups by applying a 5% solution of SDS (sodium dodecyl sulphate) for 4 hours to the cosmetic application area of the test subject, and then the mask pack of Example 1 was divided into three groups and the group B The mask pack of Example 2 was applied to the group and the mask pack of the comparative example was applied to the area of 2 x 2 on the upper left side of the arm for 6 weeks twice a day. MX18, C + K Electronics GmbH, Germany), and the degree of inflammation due to stimulation induced by SDS application was observed.

Result of anti-inflammatory effect Example 1 Erythema Example 2 Erythema Comparative Example Erythema Subject 1 180 Subject 11 210 Subject 21 250 Subject 2 215 Subject 12 195 Subject 22 277 Subject 3 230 Subject 13 178 Subject 23 255 Subject 4 167 Subject 14 208 Subject 24 210 Subject 5 255 Subject 15 168 Subject 25 211 Subject 6 141 Subject 16 123 Subject 26 233 Subject 7 177 Subject 17 210 Subject 27 245 Subject 8 230 Subjects 18 156 Subject 28 236 Subject 9 228 Subjects 19 131 Subject 29 229 Subject 10 130 Subject 20 169 Subject 30 265 Average 185.3 Average 174.8 Average 241.1

As can be seen from Table 4, the hydrogelised alginate nonwoven mask pack modified with the alginate degrading enzyme of the present invention is superior to the modified alginate nonwoven mask pack in its anti-inflammatory effect.

As described above, the hydrogel alginate nonwoven mask pack using the alginate degrading enzyme according to the present invention can be produced by modifying an insoluble calcium alginate polymer in a calcium alginate polymer fiber with a water-soluble oligomer alginate using an alginate degrading enzyme, Strengthen skin barrier, and provide anti-inflammatory effects.

In addition, by minimizing evaporation of the hydrogel cosmetic liquid through formation of micro-film of water-soluble alginate component and high water retention ability, it is possible to supply sufficient nutrition to the skin and to absorb and remove heavy metals of the skin, to inhibit harmful substances, Respectively.

Claims

An alginate nonwoven mask pack produced by blending a calcium alginate polymer fiber and a heterogeneous fiber in sheet form,
Wherein the insoluble calcium alginate polymer in the calcium alginate polymer fiber is modified with a water-soluble oligomer alginate using an alginate decomposing enzyme.

The method according to claim 1,
The modification is carried out by reacting the insoluble calcium alginate polymer with a poly (M) lyase [EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] or poly (G) lyase [EC 4.2.2.11 (1 → 4) guluronan lyase]. The alginate degrading enzyme-hydrogeled alginate nonwoven fabric mask pack according to claim 1,

The method according to claim 2,
Wherein the alginate degrading enzyme is formed so as to be contained in an essence liquid impregnated in the alginate nonwoven mask pack.

The method according to claim 3,
The alginate degrading enzyme may be selected from the group consisting of the (M) lyase (EC 4.2.2.3 (1 → 4) -β-Dmannuronanlyase] or the poly (G) lyase [EC 4.2.2.11 (1 → 4) -α-L- guluronan lyase] Or a mixture thereof in a predetermined ratio,
Wherein the essence liquid contains 0.001 ppm to 20000 ppm of the alginate decomposing enzyme. &Lt; RTI ID = 0.0 > 21. < / RTI >

The method according to any one of claims 1 to 4,
Wherein the heterogeneous fibers are at least one selected from the group consisting of regenerated fibers and natural fibers.