KR20160127328A - Food composition for improving liver function and method of health tea using thereof - Google Patents

Abstract

The present invention relates to a food composition having a liver function mitigation effect using an extract of one or more kinds selected from the group consisting of Angelica utilis Makino, reed, Erythronium japonicum Decne, Salvia Plebia, Momordica charantia L., Cornus officinalis S. et Z., red ginseng and a combination thereof, and a method for manufacturing health tea using the same. According to the present invention, health tea manufactured by using Angelica utilis Makino, reed, Erythronium japonicum Decne, Salvia Plebia, Momordica charantia L., Cornus officinalis S. et Z., and red ginseng has an excellent effect of restraining liver toxicity and an effect of mitigating a liver function, thus the health tea may be applicable to a purpose for mitigating, preventing or curing liver diseases.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a food composition for improving liver function,

The present invention relates to a composition for health functional foods for improving liver function using green tea, reed, earedge, lily of the valley, pale blue cabbage, corn oil and red ginseng, and a method for manufacturing health tea.

Modern people suffer from various stresses, lack of exercise and environmental pollution, and heart, liver and gastrointestinal functions are weakened and they easily feel fatigued and often have fragile constitution. Because of this reality, Various health supplements for improving function are popular.

Meanwhile, the liver is located between the digestive system in the human body and the systemic circulatory system, thereby functioning to defend the whole body from the exogenous substance introduced from the outside. Since the in vivo exogenous substance passes through the liver, the liver has a higher risk of being exposed to toxic substances than other organs other than nutrients. The liver is a long-term organs with excellent regeneration ability, and in the case of some damage, the liver is recovered to a sufficient level. However, in case of continuous damage, part of the liver tissue is completely destroyed, the destroyed part is not recovered to normal, and connective tissues are accumulated so that the liver is scarred and the liver function is not restored to normal. If liver damage becomes chronic, liver disease such as liver fibrosis and cirrhosis will eventually result regardless of the cause.

Recently, the death rate of liver disease among liver diseases was 23.4 per 100,000 people in Korea, and the number of deaths was 28.8 cases in chronic liver disease. In recent years, Korea National Statistical Office 56.1 per 100,000 people have been reported as the highest cause of death due to liver disease. In addition, recently, liver damage due to mental stress is increasing. In this case, the damaged hepatocytes need to be restored. However, in the urgent modern society, there is no room for mental rest, so mental stress, The body can not defend and detoxify, causing abnormalities in the immune system and causing other diseases.

Therefore, in order to solve the major causes that may affect liver disease, new products for health functional foods which can easily protect the liver together with the social phenomenon in which individual health care is important are being released.

Meanwhile, functional foods for liver protection developed until now have insufficient actual liver protection effect, and many of them use materials that can cause side effects such as synthetic compounds. Therefore, It is necessary to develop health functional food made from natural materials.

Korea patent application 2008-0058599

In the present invention, it has been found that the effect of the present invention on the natural materials which can reduce the hepatoprotective effect, that is, the hepatotoxicity and improve the liver function, is excellent in the effect of Erzeeji, Hamcho, Reed, Yeoju, And confirmed that a functional health tea can be manufactured using such a material, thereby completing the present invention.

Accordingly, an object of the present invention is to provide a composition for a health food for improving liver function, which comprises, as an active ingredient, at least one extract selected from the group consisting of Deletion, Green tea, Reed, Yeoju, Mulberry Chinese cabbage, Corn oil and Red ginseng.

Another object of the present invention is to provide a method for producing a health tea having an effect of improving liver function using green tea, green tea, reed, lettuce, mulberry Chinese cabbage, corn oil and red ginseng.

In order to provide the object of the present invention as described above, the present invention provides a method for improving liver function for improving liver function, which comprises, as an active ingredient, at least one extract selected from the group consisting of Deletion, Green tea, Reed, Thereby providing a food composition.

In one embodiment of the present invention, the extract may be water, an organic solvent or a mixed solvent extract thereof.

In one embodiment of the present invention, the organic solvent may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, and butanol.

In one embodiment of the present invention, the extract may be contained in an amount of 1 to 90% by weight based on the total weight of the composition.

In one embodiment of the present invention, the food is selected from the group consisting of tea, beverage, meat, chocolate, foods, confectionery, pizza, ramen, other noodles, gums, candy, ice cream, alcoholic beverages, Lt; / RTI >

In one embodiment of the present invention, the composition may have activity to inhibit or reduce liver damage by 1,3-dichloro-2-propanol.

The present invention also relates to a method for producing a Chinese cabbage, comprising the steps of: (a) washing a green tea plant, a reed plant, a lettuce plant, a Chinese cabbage plant, and corn oil; (b) steaming at a temperature of 100 to 130 DEG C for 15 to 30 minutes; (c) drying at a temperature of 30 to 35 DEG C for 36 to 48 hours; And (d) pulverizing the dried materials, and then adding the red ginseng slices to the mixture, followed by shaking. And a manufacturing method thereof.

The present invention relates to a composition for food having a liver function improving activity using one or more extracts selected from the group consisting of green tea, reed, leegeum, lily, pomace, Chinese cabbage, corn oil and red ginseng. The health tea prepared by using the present invention, which is prepared by using the green tea, reed, leegeum, lily, pungbean cabbage, corn oil and red ginseng, is excellent in suppressing hepatotoxicity and has an effect of improving liver function, Or for treatment.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic diagram of a method for manufacturing a health tea having liver function improving activity provided by the present invention. FIG.

The present invention is characterized by providing a composition for health food for improving liver function, which comprises, as an active ingredient, at least one extract selected from the group consisting of Irreduction, Green tea, Reed, Yeoju, Mulberry Chinese cabbage, Corn oil and Red ginseng.

Salicornia herbacea is a perennial herbaceous herbaceous plant of the dicotyledonous plant family, which grows on the beach. The stem has many nodes, is prominent, is split 1-2 times Two or three small flowers hiding in hollows on the nodes. The horns are rounded with inverted conical flesh, and grow after fencing to enclose the fruit. It is distributed in Korea, Japan, China, India and North America. In Korea, it is a one-year-old herb that absorbs seawater in the west coast and the tidal flats. It is characterized by having all ingredients in seawater. It is known that such green tea has the effects of constipation, atopy, regulation of blood purification, elimination of sukbyeon, and improvement of sexual function.

Also, reed (Phragmites communis) is a monocotyledonous plant with lemongrass crest. It is a 3-meter-high perennial plant. Its rootstock is coarse, large, long side by side and has many beard roots in its nodule and yellowish white. Stems are long and large, cylindrical, solid, gathered and straight. Leaves are 2 lines long, narrow, 50㎝ long, 4㎝ long, with sharp pointed ends, rough edges rough and green. The leaf sheath has no hair, the tongue is short, and the edge is hairy. Flowers are large cones, 15 ~ 50㎝ long, bloom at the end of the stem, many branches are split, many small spines are slightly tight, purple at first, and purplish brown later. Small ears have five flowers and are thin and long and sharp. It grows in the sandy soil around wetlands, habitats and lakes, and is distributed in the temperate temperate zone and the subduction zone in Korea and the Northern Hemisphere.

Erythronium japonicum is a perennial herbaceous plant and lily of the valley plant, and is a perennial plant. It is also called as a carpenic acid fungus, a lobster, an algae, an erge, an alegi, and a silk herb. Its scientific name is Erythronium It is japonicum (Balrer) Decne. It grows in the sunny forest of high mountain range, it takes more than seven years from the seeds to bloom and the flower blooms in March ~ May. When the petal starts to run at 17 ~ 20 ℃, Is turned back completely. The stem of the scales is fleshy in white, several of which are connected to each other and extend to the side from the ground. The stem of the flower stands up straight from the tip of the stem of the scales and runs 1-2 leaves. There are about 24 species in the world and rarely white flowers, which seems to be due to recessive genes. These horseshoe are prepared by cutting leaves in spring and then boiling them in the shade, eating them as herbs, and scaling stalks. It works to improve gastrointestinal function, so it stops vomiting and diarrhea. It is also known to be effective for headache and dizziness.

Momordica Charantia is a perennial herbaceous plant with dicotyledonous plant leaves and is native to Asian tropics. The stem is thin and grows 1-3m long, climbing up other things with a tendril. Leaves are alternate phyllotaxis, long sagging, 5-7 split edges, and split pieces again with sawteeth. The flower is a single flower, one on each side of the leaf, and yellow. The calyx is bell-shaped, the corolla is deeply divided into 5 pieces, the stamens are 3 pieces, and the style is divided into 3 pieces. The fruit is long, oval, narrow in both ends, has lumpy protrusions, ripens in yellowish red, and is irregularly split, resulting in seeds shrunken in red flesh. These lakes are mainly planted for public use.

Salvia plebeia is a perennial plant of the family Lamiaceae, and is similar to that of Bongdong duck, which is called a duckweed, a duckweed, a duckweed, a duckweed, a duckweed, a duckweed, It grows in fields, rice paddies, fields, riversides, and can be seen not difficult in large-scale apartment buildings. It is called "雪 見 草" because it grows by seeing snow when it is alive all the winter because it is alive all winter. The amusing name of pale green cabbage was rooted roots of Chinese cabbage, and leaf surface was convex. These pungent cabbages contain essential oils, saponins, unsaturated fatty acids, flavonoids, and have a distinctive odor. The seeds contain a lot of oil, which can be used for dressing in salads and for cooking dishes like cooking oil. Insect cabbage is effective for cough, sputum, rhinitis and old asthma. It is also known to be effective against women's diseases such as poor circulation and menstrual cramps.

Cornus officinalis (Cornus officinalis) is a deciduous small arboreous tree belonging to the Tertiary family. Its scientific name is Cornus officinalis S. et Z. It is 7m in height and light brown. Its bark is peeled off. Leaves are oval or ovate lance. It is 4 ~ 12㎝ long and 2.5 ~ 6㎝ wide. The surface is green and there is a little bitch. The back side is light green or white. Flowers are yellow and bloom earlier than leaves in March and April. They are beautifully grown and grown as cannabis. The fruit is long elliptical and ripens in August. The fruit contains cornin, verbenalin, tannin, ursohn, vitamin A, etc., and has a pharmacological effect. It is known that such nourishing oil is effective when you take urine frequently due to decline of reproductive function, or when you feel dizziness, dizziness, tinnitus, back and knee pain, and quiet pain.

Red ginseng is steamed and dried red ginseng. In its manufacturing process, ginseng is washed thoroughly with water, steamed in a certain container, steamed for a certain period of time using heated steam. After the first hot air drying, it is dried until the moisture reaches 12.5 ~ 13.5% by using solar heat or other methods. After picking the red hot pepper (ginseng) and sorting the shape, They are sorted by size and weight and vacuum packed in Tempreta can. The important components of red ginseng include glycosides, ginseng, panacen, polyacetylene, nitrogen compounds, flavonoid vitamins (group B), trace element enzymes, antioxidants, organic acids and amino acids. Red ginseng has sedative and excitatory effects on the central nervous system and acts on the circulatory system to prevent hypertension and arteriosclerosis. In addition, it also acts to reduce hematopoiesis and blood glucose levels, to protect the liver, to work on the endocrine system, indirectly to sexual behavior and reproductive effects, And antitumor action (anti-tumor effect), radiation protection effect, skin protection and softening action. Among the effects of red ginseng, adaptogen (aptogen) effect has the ability to enhance the ability of the living body to adapt more easily to various diseases, Scientifically proven.

The present inventors have found that the above-mentioned plant extracts can inhibit hepatotoxicity and improve liver function.

In particular, according to one embodiment of the present invention, there is provided a method for preparing an animal model, comprising administering 1,3-dichloro-2-propanol, which is a substance causing acute hepatotoxicity, Experiments were performed to determine if the damaged liver could be recovered.

As a result, it was confirmed that the body weight change due to hepatotoxicity could be restored to normal. AST, ALT, BUN, and creatinine, which are indexes for measuring liver water, As a result, it was shown that they inhibited the increase of expression of these indicators due to hepatotoxicity induction.

 These indicators are known to increase the level of hepatic damage compared to the steady state. Numerical analyzes of these indicators can be used to check the liver status. In the case of substances that can reduce these levels, It can be used as a treatable substance.

In another embodiment of the present invention, the values of active oxygen inhibitory enzyme (SOD) and catalase were measured. Here, the active oxygen inhibitory enzyme (SOD) is an enzyme having antioxidant activity, And has an effect of inhibiting the production of active oxygen by liver damage.

Catalase also has antioxidant activity, and it exists not only in the liver of our body but also in the red blood cell kidneys, and also acts to disinfect the bacteria.

In this respect, the plants used in the present invention can promote and activate the activities of the active oxygen inhibitory enzyme (SOD) and the catalase, and thus they can be very effective in improving liver function.

  Accordingly, the present invention provides a composition for health food for improving liver function, which comprises, as an active ingredient, at least one extract selected from the group consisting of green tea, green tea, reed, lettuce, lily of the valley,

The extract according to the present invention can be obtained by extracting and isolating from nature using an extraction and separation method known in the art, and the extract defined in the present invention can be used as an extract of green tea, reed, , Mung bean, Chinese cabbage, corn oil, and red ginseng, and includes, for example, a crude extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract of the plant. Preferably, it may be an extract extracted from leaves, flowers, stems and roots of the plant.

As an appropriate solvent for extracting the extract from at least one plant selected from the group consisting of the above-mentioned green tea, reed, leeches, lentil, pear-shaped Chinese cabbage, corn oil and red ginseng, any organic solvent which is pharmaceutically acceptable may be used, Or an organic solvent may be used. Examples of the organic solvent include, but are not limited to, purified water, methanol, ethanol, propanol, isopropanol, butanol, etc. (Such as acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, cyclohexane, etc.) May be used alone or in combination.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation to the production method of the extract of one or more selected from the group consisting of green tea, green tea, reed, lemonade, lemon, Chinese cabbage, corn oil and red ginseng according to the present invention, and any known method may be used.

For example, one or more kinds of extracts selected from the group consisting of green tea, reed, lees, yams, pomelo cabbage, corn oil and red ginseng contained in the composition of the present invention may be prepared by subjecting the first extract, And may be produced in a powder state by an additional process such as vacuum distillation and freeze-drying or spray-drying. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, one or more kinds of extracts selected from the group consisting of green tea, green tea, sorghum, sorghum, green tea, corn oil and red ginseng are all extracts, fractions and tablets obtained in each step of extraction, Concentrate or dried product.

In one embodiment of the present invention, at least one extract of the present invention selected from the group consisting of green tea, green tea, reed, The extract of the present invention may be contained in an amount of 1 to 90% by weight based on the total weight of the composition.

The composition of the present invention may be a food composition containing the above extract as an active ingredient. Such a food composition may contain one or more extracts selected from the group consisting of effective ingredients such as green tea, green tea, reed, And may contain various flavors or natural carbohydrates as an additional ingredient as well as ordinary food compositions.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. The above-described flavors can be advantageously used as natural flavorings (tau martin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.).

In addition, the food composition of the present invention may be formulated into a food composition containing at least one pharmaceutically acceptable or pharmaceutically acceptable carrier in addition to the above-described effective ingredients.

The form of the food composition may be a tablet, a capsule, a powder, a granule, a liquid, a ring, a liquid, a syrup, a juice, a suspension, an oil, or a drip agent. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methylcellulose, agar, bentonite, xanthan gum, and the like. Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile water and sterile water suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants can be additionally added to formulated into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

The food composition of the present invention formulated in the above manner can be used as a functional food or can be added to various foods

Foods to which the composition of the present invention can be added include, for example, foods such as tea, beverage, meat, chocolate, food, confectionery, pizza, ramen, other noodles, gums, candy, ice cream, alcoholic beverages, .

The food composition may further contain at least one nutrient, vitamins, minerals (electrolytes), a synthetic flavor and a natural flavor, in addition to one or more extracts selected from the group consisting of effective ingredients such as green tea, green tea, reed, Used in flavoring agents, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols and carbonated drinks And the like. In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks

The extract of at least one selected from the group consisting of the active ingredients of the present invention, such as green tea, green tea, sorghum, lemongrass, mackerel cabbage, corn oil and red ginseng, is a natural substance and has almost no side effects such as chemical agents. Can be used safely for long-term use for the purpose.

In addition, the present invention provides a health food for improving liver function, which comprises as an active ingredient at least one extract selected from the group consisting of green tea, green tea, reed, lettuce, lemon, Chinese cabbage, corn oil and red ginseng.

The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and circles for the purpose of improving liver function, inhibiting hepatotoxicity, or improving liver disease.

In the present invention, the term "health functional food" refers to a food prepared and processed by using raw materials or ingredients having useful functions in accordance with the Act on Health Functional Foods, and the nutrients can be regulated on the structure and function of the human body, ≪ / RTI > is intended to be taken for the purpose of obtaining a beneficial effect for health use such as action.

The health functional foods of the present invention may contain conventional food additives and, unless otherwise specified, whether or not they are suitable as food additives are classified according to the General Rules for Food Additives approved by the Food and Drug Administration, Standards and standards.

Examples of the food items included in the above food additives include chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as persimmon extract, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

For example, the health functional food in the form of tablets may contain at least one extract selected from the group consisting of the active ingredients of the present invention, such as green tea, green tea, sorghum, lemon juice, mallow cabbage, corn oil and red ginseng as excipients, binders, Or the like can be granulated by a conventional method, followed by compression molding with a lubricant or the like, or the mixture can be directly compression-molded. In addition, the health functional food of the tablet form may contain a mating agent or the like if necessary.

The hard capsule of the capsule type health functional food is prepared by adding one or more kinds of extracts selected from the group consisting of green tea, green tea, reed, green tea, And the soft capsule can be prepared by filling the mixture obtained by mixing the extract with an additive such as an excipient into a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The health functional food of the ring form is a mixture of one or more extracts selected from the group consisting of green tea, green tea, reed, lemon, Chinese cabbage, corn oil and red ginseng which is an effective ingredient of the present invention, excipient, binder, , And may be coated with saccharides or other skin-protecting agents according to need, or may be coated with a substance such as starch or talc.

The granular health functional food may be a mixture of at least one extract selected from the group consisting of green tea, green tea, sorghum, sorghum, green tea, corn oil and red ginseng as active ingredients of the present invention, excipient, binder, , And if necessary, may contain a flavoring agent, a mating agent, and the like.

The health functional food may be a beverage, a meat, a chocolate, a food, a confectionery, a pizza, a ramen, a noodle, a gum, a candy, an ice cream, an alcoholic beverage, a vitamin complex and a health supplement food.

The present invention can also provide a method for producing a health tea having an effect of improving liver function using green tea, reed, sorghum, lemon, Chinese cabbage, corn oil and red ginseng.

A method for manufacturing a health tea according to the present invention comprises the steps of: (a) washing a green tea plant, a reed plant, a lettuce plant, (b) steaming at a temperature of 100 to 130 DEG C for 15 to 30 minutes; (c) drying at a temperature of 30 to 35 DEG C for 36 to 48 hours; And (d) pulverizing the dried materials, and then adding and filtering the red ginseng extract powder.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

≪ Example 1 >

Production of health tea having liver function improving activity

The inventors of the present invention produced a health tea having liver function improving effect by using Hamcho, Reed, Ergijie, Yeoju, Gombo Chinese cabbage, Corn oil and Red ginseng. The mixing ratio of each material and manufacturing process are as described below.

Health tea  Mixing ratio of materials for manufacturing ingredient Compounding ratio (% by weight) Green tea 23 Reed 23 Irregular 23 Yeoju 10 Pale green cabbage 9 Corn oil 9 Section of red ginseng 3 system 100.0

Table 1 shows the blending ratio of each material for health tea production of the present invention. The blended bean sprouts were made with green tea, green tea, reed, and functional reinforcing materials such as Momongo cabbage, Sansui oil and Yeoju. These materials were purchased from a general market and then lyophilized. Each raw material was finely cut into individual pieces, weighed, mixed at the above ratios, and then subjected to the following squeezing process.

In addition, the red ginseng slices added in the following manufacturing process were obtained from Jinan Red Ginseng Research Institute, and the red ginseng was used as a raw material and the slices prepared according to the general methods known in the art were used.

Health tea manufacturing process

The manufacturing process of the health tea according to the present invention is shown in FIG. 1 as a schematic diagram. First, (1) washing of green tea, reed, sorghum, lily of the valley, Chinese cabbage, and marine oil, removing water and cutting thinly; (2) putting in a boiling pot at a temperature of 121 캜 for 20 minutes; (c) cooling in a well-ventilated room at about 35 to 40 DEG C, and then drying in a dryer at a temperature of 30 to 35 DEG C for 36 to 48 hours; And (d) pulverizing each dried material so that the particles become slightly coarse, adding the red ginseng slices, kneading the mixture at 130 to 100 ° C. for 10 to 15 minutes using a shaker, cooling, packing in a container Lt; / RTI >

<Experimental Example 1>

Hepatotoxicity improvement efficacy analysis

 The inventors of the present invention conducted the following experiments in order to confirm whether hepatotoxicity improving efficacy of each herbal prepared health tea used for the preparation of the functional tea for improving liver function in the above examples is present.

<1-1> Experimental animal  And breeding conditions

  The experimental animals of this study were 269.5 ± 16.54 g in average body weight, and 7-week-old Sprague-Dowley male rats were purchased and healthy individuals were selected after a week of purifying period. The incubation environment was kept constant for the duration of the experiment with temperature 23 ± 2 ℃, humidity 55 ± 10%, illuminance 200 ~ 300 Lux and illumination time 12 hours (lit: 06:00, dark: 18:00). The feed and water were supplied in an unlimited amount to allow for free ingestion.

<1-2> Test substance administration

Experimental animals were divided into two groups: a group administered alone with 1,3-Dichloro-2-propanol (1,3-DCP Sigma-Aldrich Co., MO, USA) (2%, 2%, 2%, 2%, 2%, 2%, 5%, 2%, 2%, 2%, 2%, 2% and 5%, respectively) of tea, green tea, , Tea prototype 2%, tea prototype 5%), the following experimental method was applied to each experimental group to confirm liver toxicity improvement effect of each herb and health tea produced. At this time, each sample extract was dissolved according to the dose (10 ml / kg) calculated on the basis of the individual body weight by calculating the 2% and 5% doses of the experimental animals.

<1-3> General symptom observation and weight measurement according to the test substance administration

During the test period, the animals were observed for abnormalities, clinical symptoms, and deaths at a fixed time once a day. The body weight was measured on the 7th day after the end of the test substance administration, and once again before the autopsy, the amount of gain before and after acute hepatotoxicity caused by 1,3-DCP administration was calculated.

<1-4> Serum biochemical test

After 24 hours of subcutaneous administration of 1,3-DCP, ketamine (Ketamine HCl; Yuhan Co., Korea, 40 mg / kg) and xylazine (Rompun; Bayer Korea, Korea, 10 mg / kg) The animals were anesthetized and then laparotomized and collected in the posterior vena cava. The collected blood was placed in a serum separation tube, left at room temperature for 30 minutes, and centrifuged at 3000 rpm for 15 minutes to separate the serum. Serum biochemical tests were performed by using asparatate aminotransferase (AST), alanine aminotransferase (ALT), BUN, and creatinine concentrations in a biochemical analyzer (Drichem 4000i; Fujifilm Co., Japan) Respectively.

<1-5> Long term weighing

Each of the experimental animals was bled and sacrificed. The liver, left and right kidneys were weighed and weighed and the relative weights were calculated.

<1-6> Measurement of oxidation-related enzyme activity

Hepatic tissues were extracted from each experimental group and the liver tissues were partially washed with phosphate buffered saline (PBS) to measure superoxide dismutase (SOD) and catalase activity, and stored in a freezer at -80 ° C. To measure the enzyme activity, 0.25 M sucrose buffer (pH 7.5) was added at a ratio of 4 times per 1 g of stored liver tissue and homogenized with a homogenizer (10,000 g, 2 minutes) under ice-cooling. The homogenate was centrifuged at 600 ° C for 10 minutes at 4 ° C to remove the nuclear and microbial components. The supernatant was centrifuged at 15,000 × g for 20 minutes at 4 ° C to separate the coenzyme source. SOD activity was measured by a reaction of 50 mM carbonic buffer (pH 10.2), 0.1 mM EDTA, 0.1 mM Xanthine, 0.025 mM nitroblue tetrazolium and a coenzyme source at 25 ° C for 10 minutes according to Beauchamp and Fridovin (1971) xanthine oxidase (3.3 · 10 ~ 6 mM) was added to measure the degree of photo-degradation of NBT at 550 nm. Catalase activity was measured by adding 10 mM H 2 O 2 and coenzyme source to 50 mM potassium phosphate (pH 7.0) according to the method of Aebi (1984) and then observing the change of absorbance at 240 nm for 2 minutes. At this time, the amount of enzyme that decomposes 1uM of H 2 O 2 for 1 minute was 1 unit.

<1-7> Histopathological examination

The remaining portion of liver tissue was fixed in 10% neutral buffered formalin solution, and then normal tissue sections and hematoxylin-eosin staining were performed. After staining, histological abnormalities were observed under an optical microscope.

<1-8> Statistical analysis

The results obtained from each of the above experiments are shown in GraphPad InStat v. 3.0 (GraphPad Software, Inc., Ca, USA) program. Statistical significance of mean values between groups was tested by Dunnett's multiple comparison method at 5% and 1% after one-way ANOVA.

<Result 1>

Analysis of each herb used for the production of functional health tea

In the above experimental example, the results of the above-mentioned analysis on the green tea, green lettuce and reed extract of the experimental animals were as follows.

<1-1> Measurement of weight change

First, the body weight of each test animal was measured and the results are shown in Table 2 below. Comparing the body weights before and after 1,3-DCP, there was no statistically significant difference between the control and the 1,3-DCP alone group and the respective sample extract-administered groups. However, the changes in body weight before and after the administration of 1,3-DCP were comparable to those of the control group in the 1,3-DCP alone group, the Hamcho 2% group, the Irregular 2% group, the Irregular 5% group, the Reed 2% group and the Reed 5% And the weight gain was significantly decreased.

In addition, the body weight gain of the 5% green tea group was significantly lower than that of the control group, but was significantly higher than that of the 1,3-DCP alone group, and the weight gain of the 2% 3-DCP alone group (p <0.05). In the 5% healed group, the body weight gain was significantly decreased compared to the control group, but the statistically significant increase (p <0.01) was observed compared with the 1,3-DCP alone group.

Weight change measurement Before 1,3-DCP administration After 1,3-DCP administration Weight gain Control group 296.3 ± 12.45 303.5 ± 16.41 8.6 ± 2.48 1,3-DCP alone 310.0 ± 14.18 297.5 ± 12.01 -12.6 + 4.87 ^** 1,3-DCP + green tea 2% 309.3 ± 10.32 301.1 ± 9.56 -6.7 + 4.92 ^** 1,3-DCP + green tea 5% 303.5 ± 299.7 299.7 ± 19.47 -3.8 ± 5.30 ^** , ^†† 1,3-DCP + sorbitol 2% 302.3 ± 26.79 296.2 ± 29.55 -6.1 + 7.64 ^** 1,3-DCP + Irregular 5% 311.1 + - 7.71 304.5 ± 13.35 -6.6 ± 8.89 ^** 1,3-DCP + heel 2% 313.9 ± 13.90 308.5 ± 10.25 -5.3 ± 5.52 ^** , ^† 1,3-DCP + helix 5% 311.7 ± 17.19 308.9 ± 18.01 -2.8 ± 5.83 ^** , ^†† 1,3-DCP + Reed 2% 305.3 ± 7.73 300.0 ± 11.18 -5.2 ± 5.51 ^** 1,3-DCP + Reed 5% 306.1 ± 15.25 298.6 ± 11.19 -8.2 ± 8.27 ^**

Values are presented as means ± SD (g) of 7 rats.

^* Significant difference at P <0.05 when compared with the control group.

^** Significant difference at P <0.01 level when compared with the control group.

^† Significant difference at P <0.05 level when compared with the 1,3-DCP group.

^†† Significant difference at P <0.01 level when compared with the 1,3-DCP group.

<1-2> Serological analysis results

The results of serological analysis are shown in Table 3 below.

As a result, AST, ALT and BUN levels were significantly increased in 1,3-DCP-treated group compared to the control group, whereas AST and ALT levels were 1, 3-DCP alone group and the BUN level was significantly decreased (p <0.05).

AST, ALT, and BUN levels were significantly lower in the 2%, 2%, and 2% healed groups compared to the 1,3-DCP alone group. , 3-DCP alone, and the BUN level was significantly decreased (p <0.05). In addition, AST, ALT and BUN values were significantly decreased in the 5% reed group compared to the 1,3-DCP alone group.

Serological analysis results AST ALT BUN Creatine Control group 165.6 ± 27.87 65.3 ± 34.89 21.1 ± 2.77 0.4 ± 0.08 1,3-DCP alone 16326.7 ± 2815.80 ^** 11853.3 ± 7052.20 ^** 36.3 ± 14.52 ^** 0.6 ± 0.41 1,3-DCP + green tea 2% 650.0 ± 521.66 ^†† 1080.0 ± 1036.40 ^†† 23.3 ± 6.19 ^† 0.3 ± 0.13 1,3-DCP + green tea 5% 300.0 ± 116.62 ^†† 355.0 ± 198.91 ^†† 22.6 ± 5.58 ^† 0.3 ± 0.12 1,3-DCP + sorbitol 2% 1816.0 ± 2793.30 ^†† 1516.0 占 1805.60 ^†† 18.4 ± 1.21 ^†† 0.3 ± 0.08 1,3-DCP + Irregular 5% 2524.0 ± 2308.00 ^†† 1720.0 ± 2188.01 ^†† 20.0 ± 2.92 ^†† 0.3 ± 0.06 1,3-DCP + heel 2% 828.0 ± 520.88 ^†† 972.0 ± 695.93 ^†† 19.6 ± 3.04 ^†† 0.3 ± 0.08 1,3-DCP + helix 5% 360.0 ± 247.79 ^†† 404.0 ± 228.65 ^†† 15.9 ± 2.47 ^†† 0.3 ± 0.08 1,3-DCP + Reed 2% 384.0 ± 194.11 ^†† 488.0 ± 327.90 ^†† 23.4 ± 2.46 ^† 0.3 ± 0.06 1,3-DCP + Reed 5% 2330.0 ± 1579.90 ^†† 1570.0 ± 704.56 ^†† 19.5 ± 2.63 ^†† 0.3 ± 0.08

Values are presented as means ± SD of 7 rats.

^* Significant difference at P <0.05 when compared with the control group.

^** Significant difference at P <0.01 level when compared with the control group.

^† Significant difference at P <0.05 level when compared with the 1,3-DCP group.

^†† Significant difference at P <0.01 level when compared with the 1,3-DCP group.

AST, aspartate aminotransferase; ALT, alanine aminotransferase.

<1-3> Liver and kidney weight analysis

The liver and kidney were excised and the weight was measured. The results are shown in Table 4 below. The relative weight of liver was significantly decreased (p <0.05) in 2% green tea group. The relative weight of liver in the 2% healing group was decreased statistically compared with that of the control group and the relative weight of the liver was decreased in the 5% healing group compared to the control group (p <0.05).

Liver and kidney weight analysis Absolute weight Relative weight liver kidney liver kidney Control group 13.3 ± 0.84 2.2 ± 0.14 4.4 ± 0.24 0.7 ± 0.05 1,3-DCP alone 12.2 ± 0.61 2.2 ± 0.08 4.1 ± 0.10 0.7 ± 0.04 1,3-DCP + green tea 2% 11.7 ± 0.45 2.3 ± 0.15 3.9 ± 0.10 ^* 0.8 ± 0.06 1,3-DCP + green tea 5% 13.4 ± 2.18 2.4 ± 0.35 4.4 ± 0.64 0.8 ± 0.09 1,3-DCP + sorbitol 2% 12.7 ± 2.36 2.3 ± 0.28 4.2 ± 0.59 0.8 ± 0.08 1,3-DCP + Irregular 5% 13.1 ± 1.58 2.4 ± 0.22 4.3 ± 0.67 0.8 ± 0.08 1,3-DCP + heel 2% 12.0 + 1.04 2.2 ± 0.19 3.9 ± 0.36 0.7 ± 0.0 1,3-DCP + helix 5% 12.1 ± 0.93 2.4 ± 0.22 4.0 ± 0.12 ^* 0.8 ± 0.09 1,3-DCP + Reed 2% 12.1 ± 0.98 2.3 ± 0.17 4.0 ± 0.20 0.8 ± 0.05 1,3-DCP + Reed 5% 12.7 ± 1.33 2.4 ± 0.31 4.3 ± 0.52 0.8 ± 0.11

Values are presented as means ± SD (g) of 7 mice.

^* Significant difference at P <0.05 when compared with the control group.

^** Significant difference at P <0.01 level when compared with the control group.

^† Significant difference at P <0.05 level when compared with the 1,3-DCP group.

^†† Significant difference at P <0.01 level when compared with the 1,3-DCP group.

<1-4> SOD and catalase  Activity measurement

The results of measurement of SOD and catalase activity in liver tissues for each experimental group are shown in Table 5 below.

As a result, catalase activity and SOD activity were significantly decreased in the group treated with 1,3-DCP alone, 2% with Hamcho, 5% with Hamcho, and 2% with irrigation, Compared to the control group, but significantly increased compared to the 1,3-DCP group, and SOD activity was significantly decreased compared with the control group. The activity of catalase and SOD in the 2% heaer group was significantly lower than that of the control group. The activity of catalase in the 5% healed group was significantly lower than that of the control group and was significantly higher than that of the 1,3-DCP group , SOD activity was significantly decreased compared with the control group. In the 2% reed group, catalase and SOD activity were significantly decreased compared to the control group. In the 5% reed group, the catalase activity was significantly decreased compared to the control group, and the SOD activity was significantly decreased compared with the 1,3-DCP group and the SOD activity was significantly decreased compared to the control group.

SOD and catalase activity Catalase
(units / mg protein) Superoxide dismutase
(units / mg protein) Number of experimental animals (rat) Control group 11.49 + 1.415 ^a 174.6 ± 33.88 7 1,3-DCP alone 2.40 ± 0.626 ^** 83.3 ± 14.01 ^{** 7} 1,3-DCP + green tea 2% 1.65 ± 0.317 ^** 81.5 ± 14.41 ^{** 7} 1,3-DCP + green tea 5% 3.08 ± 0.722 ^** 96.8 ± 18.01 ^{** 7} 1,3-DCP + sorbitol 2% 2.92 ± 0.638 ^** 93.1 ± 16.17 ^{** 7} 1,3-DCP + Irregular 5% 4.59 ± 0.712 ^{**, ††} 104.4 ± 16.64 ^{** 7} 1,3-DCP + heel 2% 2.77 ± 0.339 ^** 77.1 ± 18.64 ^{** 7} 1,3-DCP + helix 5% 5.65 ± 0.775 ^{**, ††} 108.4 ± 28.77 ^{** 7} 1,3-DCP + Reed 2% 0.82 ± 0.206 ^** 76.5 ± 23.14 ^{** 7} 1,3-DCP + Reed 5% 4.35 ± 0.597 ^{**, ††} 100.4 ± 20.21 ^{** 7}

^{a Values are presented as means ± SD} .

^** Significant difference at P <0.01 level when compared with the control group.

^†† Significant difference at P <0.01 level when compared with the 1,3-DCP group.

<Result 2>

Analysis of the functional tea prepared by the method of the present invention

The experimental results for each animal group administered with the functional tea prepared by the method of the present invention in place of each herb in the above Experimental Example are shown below.

<2-1> Measurement of weight change

The weight change after administration to the test animals was measured with 2% and 5% of the functional tea prepared by the method of the present invention, and the results are shown in Table 6 below. The results showed that the body weight change due to 1,3-DCP administration was decreased in the tea 2% and tea 5% groups.

Values are presented as means ± SD (g) of 7 rats.

^* Significant difference at P <0.05 when compared with the control group.

^** Significant difference at P <0.01 level when compared with the control group.

^† Significant difference at P <0.05 level when compared with the 1,3-DCP group.

^†† Significant difference at P <0.01 level when compared with the 1,3-DCP group.

<2-2> Serological analysis results

Serological analysis was carried out on the group administered with 2% and 5% of the tea prepared by the method of the present invention, instead of the herb extract administered in the above Experimental Example, and the serum analyzed The same method was used for the analysis.

As a result of the analysis, as shown in Table 7, the AST, ALT and BUN levels were significantly increased in the 1,3-DCP alone group compared with the normal control group. However, in the group administered with 2% , And the levels of ALT and BUN were also significantly lower than those of the 1,3-DCP alone group. In addition, the AST, ALT, and BUN values in the group treated with 5% of the 2% of the control group were decreased to be closer to the normal control group.

Values are presented as means ± SD of 7 rats.

^* Significant difference at P <0.05 when compared with the control group.

^** Significant difference at P <0.01 level when compared with the control group.

^† Significant difference at P <0.05 level when compared with the 1,3-DCP group.

^†† Significant difference at P <0.01 level when compared with the 1,3-DCP group.

AST, aspartate aminotransferase; ALT, alanine aminotransferase.

<2-3> Liver and kidney weight analysis results

The liver and kidney were excised and the weight was measured. The results are shown in Table 8 below. As a result, the relative weights of the 2% and 5% groups were slightly decreased (p <0.05) compared to the control group, but no significant changes were observed.

Values are presented as means ± SD (g) of 7 mice.

^* Significant difference at P <0.05 when compared with the control group.

^** Significant difference at P <0.01 level when compared with the control group.

^† Significant difference at P <0.05 level when compared with the 1,3-DCP group.

^†† Significant difference at P <0.01 level when compared with the 1,3-DCP group.

<2-4> SOD and catalase  Activity measurement

The results of measurement of SOD and catalase activity in liver tissues for each experimental group are shown in Table 9 below.

The activity of SOD and catalase was measured in liver tissues. The activity of catalase was increased by the treatment of 1,3-DCP in 2% group, and the activity of 1,3- DCP-treated group. The degree of increase in SOD and catalase activity in the liver tissues of the tea prepared by the method of the present invention was found to be proportional to the content, that is, it was found that the activity was further increased in the 5% .

In summary, based on the results of the above-described Examples and Experimental Examples of the present invention, the inventors of the present invention have found that the use of green tea, herring, herring, reeds and functional health foods prepared using them can improve hepatotoxicity, And thus can be usefully used for the improvement, prevention or treatment of liver disease.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (6)

A composition for a health food for improving liver function, comprising an extract of Erysiphe, Green tea, Reed, Yeoju, Korean cabbage, corn oil and red ginseng as active ingredients. The method according to claim 1,
Wherein the extract is water, an organic solvent or a mixed solvent thereof. 3. The method of claim 2,
Wherein the organic solvent is selected from the group consisting of methanol, ethanol, propanol, isopropanol, and butanol. The method according to claim 1,
Wherein the extract is comprised between 1 and 90% by weight based on the total weight of the composition. The method according to claim 1,
Wherein the food is selected from the group consisting of tea, beverage, meat, chocolate, foodstuff, confectionery, pizza, ramen, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes and health supplements. The method according to claim 1,
Wherein the composition has an activity of inhibiting or reducing liver damage by 1,3-dichloro-2-propanol.

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