KR101717431B1 - Healthy food composition having anti-obesity and anti-diabetic activities - Google Patents

Abstract

The present invention relates to a food composition containing a natural plant-derived extract having antioxidant and anti-obesity activity as an active ingredient, and a health functional food. More specifically, the present invention relates to a food composition and a health functional food, Wherein the extract is at least one selected from the group consisting of corn oil, soybean oil, and corn oil. The extract of the present invention has both anti-obesity effect and excellent blood-sugar-lowering effect through amylase, glucosidase and lipase inhibitory activity acting on fat and carbohydrate metabolism, Can be usefully used as an anti-obesity and / or anti-diabetic functional food composition.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a health food composition having anti-obesity and anti-diabetic activity,

The present invention relates to a food composition comprising a natural plant-derived extract having an anti-obesity and an anti-diabetic activity as an active ingredient, and a health functional food.

Recently, the improvement of living standards due to economic development improves the hygiene environment and frequent instant food intake and eating habits change the eating habits of excessive calories. However, changes in dietary habits of these modern people tend to be accompanied by an increase in obesity population due to lack of exercise and lack of exercise. Obesity has been reported to cause breast cancer, uterine cancer and colon cancer as well as adult diseases such as hypertension, diabetes, hyperlipidemia, coronary artery disease and the like due to the persistence of obesity as well as external problems. [J. Biol. Chem., 273, 32487-32490 (1998); Nature, 404, 652-660 (2000).

Obesity has increased by 75% globally since 1980 and 33% and 26% of the population in the United States are reported to be overweight and obese respectively (Ahn IS, Park KY, Do MS 2007. Weight control mechanisms antiobesity functional agents. J Korean Soc Food Sci Nutr 36: 503-513.). In Korea, the obesity population is steadily increasing. As a result of the 2007 National Health and Nutrition Examination Survey, the obesity population has increased from 26.3% in 1998 to 31.7% in 2005.

These obesity is caused by the imbalance of energy intake and consumption, and the extra energy is converted into fat cells and stored in the body. Free fatty acids and cytokines secreted from accumulated adipocytes cause insulin resistance and increase the inflammatory response and are a direct cause of the onset of chronic diseases such as metabolic syndrome, diabetes, cardiovascular disease and cancer. In order to treat obesity, exercise habits, improvement of dietary habits through diet, drug therapy, and surgical treatment are introduced. Among the anti-obesity drugs that inhibit obesity, more than 100 kinds of drugs are sold in the US It is under development and the market size is expected to grow.

Current treatments for treating obesity include drugs that act on the central nervous system, affect appetite, and drugs that act on the gastrointestinal tract to inhibit absorption. Drugs such as fenfluramine and dexfenfluramine which inhibit the serotonin (5HT) nervous system, drugs such as ephedrine and caffeine through the noradrenergic nervous system, and recently, drugs acting on the serotonin and noradrenergic nervous system And sibutramine, which acts to inhibit obesity, are on the market. Other drugs that inhibit obesity by acting on the gastrointestinal tract include orlistat, which has recently been approved as a treatment for obesity by inhibiting lipase produced by the pancreas and reducing fat absorption.

However, among the drugs that have been used, fenfluramine has been recently banned due to side effects such as primary pulmonary hypertension and heart valve lesions. Sibutramine has side effects of increasing blood pressure, and Orlistat has a digestive tract disorder, Incontinence, and absorption of fat-soluble vitamins. In addition, other chemical synthetic drugs have problems such as decreased blood pressure and lactic acidosis, and thus they can not be used for patients suffering from heart failure or renal disease.

Therefore, in order to find a better treatment and prevention of obesity with a small side effect, there has recently been an increasing interest in a therapeutic agent for obesity of natural materials or an anti-obesity health functional food which acts to inhibit the accumulation of body fat such as plant compounds.

Accordingly, the present inventors focused their attention on the pharmacological effects of herbs, herring, green tea, reed, lotus, ladybug, lepidopteran, fig, citron and marine oil while searching for a natural substance having excellent anti- As a result of the experiment, the plant extracts showed anti-obesity effect and ameliorated an excellent blood glucose lowering effect through the amylase, glucosidase and lipase inhibiting activity acting on fat and carbohydrate metabolism, The present invention has been completed.

Korean Patent Publication No. 2004-0016578

Accordingly, an object of the present invention is to provide a food composition which is free from adverse effects on the living body and is excellent in anti-obesity and antidiabetic activity.

Another object of the present invention is to provide a health functional food which is excellent in anti-obesity and anti-diabetic activity without adverse effects on the living body and can effectively prevent or ameliorate obesity.

In order to achieve the object of the present invention as described above, the present invention provides an anti-inflammatory agent comprising an extract of at least one member selected from the group consisting of a herring, a herb, a green tea, a reed, a lotus flower, Obesity and antidiabetic food composition.

In one embodiment of the present invention, the extract may be water, an organic solvent or a mixed solvent extract thereof.

In one embodiment of the present invention, the organic solvent may be selected from the group consisting of methanol, ethanol, propanol, isopropanol, and butanol.

In one embodiment of the present invention, the extract may be contained in an amount of 0.1 to 95% by weight based on the total weight of the composition.

The present invention also provides a health functional food for preventing or ameliorating obesity containing, as an active ingredient, one or more extracts selected from the group consisting of a herring, a lemonade, a green tea, a reed, a lotus flower, a lemonade, a peppermint, a fig, a citron and a marine oil.

In one embodiment of the present invention, the extract may have the effect of preventing or ameliorating obesity through amylase, glucosidase and lipase inhibitory activities.

In one embodiment of the present invention, the food is selected from the group consisting of beverage, meat, chocolate, foods, confectionery, pizza, ram noodles, gums, candy, ice cream, alcoholic beverages, .

The one or more extracts selected from the group consisting of the herring, the herring, the green tea, the reed, the lotus, the pale green cabbage, the lily of the pine, the lentil, the fig, the citron and the marine oil of the present invention are amylase, glucosidase and lipase inhibition affecting the fat and carbohydrate metabolism The composition can simultaneously exhibit an excellent blood sugar lowering effect as well as an anti-obesity effect through its activity. Therefore, a composition containing these as an active ingredient can be usefully used as an anti-obesity and / or anti-diabetic functional food composition.

(A): water + amylose control (B): methanol + amylose control group (C): water by plant type Extract + Amylose, (D): Methanol Extract by Plant Type + Amylose, 1: Salicornia herbacea , 2: Corylopsis coreana (Stem), 3: Corylopsis coreana (Flower), 4: Erythronium japonicum (Leaf), 5: Erythronium japonicum (Root), 6: Erythronium japonicum (Flower), 7: Phragmites communis (Root), 8: Phragmites communis (stem), 9: Nelumbo nucifera (Leaf), 10: Salvia plebeia , 11: Momordica Charantia , 12: Portulaca oleracea , 13: Ficus carica (Leaf), 14: Citrus junos (Pericarp), 15: Citrus junos (Leaf), 16: Cornus officinalis ].

The present invention provides an anti-obesity and anti-diabetic functional food composition containing, as an active ingredient, at least one extract selected from the group consisting of a herring, a herb, a green tea, a reed, a lotus flower, .

'Herry ( Corylopsis coreana ) 'is a member of the genus Streptomycetes, which is distributed in East Asia and rarely in North America and Central America. There are 29 kinds of herring in Japanese apricot tree, 5 species in Japan and 5 species in India, and one species is growing in our country. Korea is a deciduous broad - leaved shrub with a scientific name of Corylopsis coreana Uyeki, and Korean name is korean winter hazel . The herb of Korea was found in Songgwangsa Temple in Sunchon, Jeonnam Province in 1910, and it is a species registered as a special plant in the academic world in 1924. This herb is a deciduous broad-leaved shrub that grows up to 1 to 5 meters in length and forms a cluster with a large number of closely-spaced buds, forming a large group of trees. They grow well in azalea along with azaleas, quinques, It is strong, and it is known that it grows well in dry soil because it does not wear the East Sea at below minus 30 degrees,

' Erythronium japonicum) 'is also called flowering plants statement monocotyledonous plants river liliales lilies as a perennial, leg folic sleep (車前葉山慈姑), lobster Whatsoever, jeweler branch, Yerevan branch, reel exchanger, silk herbs scientific name is Erythronium japonicum ( Balrer) is Decne . It grows in the sunny forest of high mountain range, it takes more than seven years from the seeds to flower to bloom, and the flower blooms from March to May. When the petal starts to run at 17 ~ 20 ℃, Is turned back completely. The stem of the scales is fleshy in white, several of which are connected to each other and extend to the side from the ground. The stem of the flower stands up straight from the tip of the stem of the scales and runs 1-2 leaves. There are about 24 species in the world and rarely white flowers, which seems to be due to recessive genes. These horseshoe are prepared by cutting leaves in spring and then boiling them in the shade, eating them as herbs, and scaling stalks. It works to improve gastrointestinal function, so it stops vomiting and diarrhea. It is also known to be effective for headache and dizziness.

'Green tea ( Salicornia Herbacea 'is a perennial herbaceous plant. It grows on the beach with its branches and branches. It divides 1-2 times and 2-3 or so small flowers are hidden in the hollows on the nodes. The horns are rounded with inverted conical flesh, and grow after fencing to enclose the fruit. It is distributed in Korea, Japan, China, India and North America. In Korea, it is a one-year-old herb that absorbs seawater in the west coast and tidal flats. It is characterized by having all ingredients in seawater. It is known that such green tea has the effects of constipation, atopy, regulation of blood purification, elimination of sukbyeon, and improvement of sexual function.

' Phragmites communis ' is a monocotyledonous plant with a bole crest of 3m in height. Its rootstock is coarse, large, long and horizontally, with a large number of hairs in its nodules and yellowish white. Stems are long and large, cylindrical, solid, gathered and straight. The leaves are 2 lines long, narrow, 50㎝ long, 4㎝ long, with sharp ends, sharp edges, rough and green. The leaf sheath has no hair, the tongue is short, and the edge is hairy. Flowers are large cones, 15 ~ 50㎝ in length, bred at the ends of the stem, many branches are split, many small spines are slightly tight, first purple, and then purple. Small ears have five flowers and are thin and long and sharp. It grows as a community in wetlands, in the sandy areas around the lake, and in temperate, temperate and sub-tropical regions of Korea and the Northern Hemisphere.

'Lotus ( Nelumbo nucifera ) is a perennial herb that is called "kite" and is a white, thin, long mud, with a thick end at the end and a long petiole of leaves. There are leaves and leaves on the surface of the water. The leaf is round and has a shield shape on the petiole with a diameter of about 30cm. The flower is red or white, and it blooms alone on the peduncle in summer. There are many pieces of perianth, the number of operations is large, and the operating table is thread-shaped. Such a lotus flower (= kite) is used as a medicinal herb, which is called as the federal medicine, and it is used as a medicinal tonic. It is known to be used in chronic diarrhea and heart disease by mixing with herbal medicine.

' Salvia plebeia ') is a perennial plant of the family Lamiaceae, and is called as a fungus, a fungus, a fungus, a fungus, a fungus, and a fungus. It grows in fields, rice paddies, fields, riversides, and can be seen not difficult in large-scale apartment buildings. Because it is alive throughout the winter, it is called a sister dog (雪見 草) because it grows by seeing a sister's 草 (冬 生 草). The amusing name of pale green cabbage was rooted roots of Chinese cabbage, and leaf surface was convex. These pungent cabbages contain essential oils, saponins, unsaturated fatty acids, flavonoids, and have distinctive odors. The seeds contain a lot of oil, which can be used for dressing in salads and for cooking dishes like cooking oil. Insect cabbage is effective for coughing, phlegm, rhinitis and old asthma. It is also known to be effective against women's diseases such as poor circulation, menstrual cramps and uterine diseases.

' Momordica Charantia 'is an annual vine plant with dicotyledonous plant leaves and is native to Asian tropics. The stem is thin and grows 1-3m long, climbing up other things with a tendril. Leaves are alternate phyllotaxis, long sagging, 5-7 split edges, and split pieces again with sawteeth. The flower is a single flower, one on each side of the leaf, and yellow. The calyx is bell-shaped, the corolla is deeply divided into 5 pieces, the stamens are 3 pieces, and the style is divided into 3 pieces. The fruit is long, oval, narrow in both ends, has lumpy protrusions, ripens in yellowish red, and is irregularly split, resulting in seeds shrunken in red flesh. These lakes are mainly planted for public use.

' Portulaca oleracea 'is a perennial herb that lives in the middle of a flowering plant and is called the purslane in English and verdolaga in Spanish. It is a juicy and well-spreading herb, It has leaves. It is a mandarin plant which is planted in a flowerbed or potted plant from China and India. Its height is 20-30cm, it has no hair, it is fleshy, its meat is white, its stem is reddish brown and many stems from hornstroke are slanted sideways It spreads and grows. It is known that these pimples contain a lot of omega-3 fatty acids and vitamin E.

' Ficus carica ) 'is a nectarine tree and a deciduous broad-leaved shrub, and it is called a fig tree because it grows in a foliage from spring to summer and mature with fruit. It is 2 ~ 4m in height and thick and brown or green brown. Leaves are wide and ovate with length of 10 ~ 20㎝ and deeply divided into 3 ~ 5. The fruit is edible or medicinal. In Korea, it grows well in the warm, deep and fertile places south of Chungcheongnam-do, and it has a good habit that my composition is good and the trees bloom. These figs contain large amounts of proteolytic enzymes that help digestion after eating meat, and are known to have a special effect on constipation. Because it has the efficacy of gun stomach, scab, and detoxification, it is used as a treatment for indigestion, anorexia, enteritis, constipation, dysentery in one room.

Citrus junos ) is an evergreen shrub with dicotyledonous plants and is known as the origin of the upstream Yangtze River in China. It grows about 3-5 meters in height and has long, sharp spines on the branches of green. Leaves are alternate phyllotaxis, long elliptical, pointed toward the tip of the leaf, slightly concave at the tip, and wavy sawtooth on the edge. Its fruit is yellow, ripe, the surface is rough and strong, but it is strong in sourness, so it is used as a spice for food rather than eating rawly.

'Cornus ( Cornus) offici nalis) is a deciduous subfamily belonging to the Tertiary family, and its scientific name is Cornus officinalis S. et Z. Its height is 7m, light brown, but its bark is peeled off, and the leaves face oval, elliptical or ovate lanceolate. Length is 4 ~ 12㎝, width is 2.5 ~ 6㎝, surface is green and there is little bitch (伏 毛). The back side is light green or white. Flowers are yellow and bloom earlier than leaves in March-April, and they are beautifully grown as cannibals. The fruit is long elliptical and ripens in August. The fruit contains cornin, verbenaulin, tannin, ursohn, vitamin A, etc., and has a pharmacological effect. It is known that such acidic milk is effective when taking urine frequently due to renal function and decline of reproductive function, or when you are experiencing pain in your back, knee, waist and knee joints.

The present inventors have experimentally proved that the above plant extracts have amylase, glucosidase and lipase inhibitory activity and are excellent in blood glucose lowering effect. Thus, the present inventors have found that the above extracts are effective as an active ingredient of an antiobesity and / or antidiabetic functional food composition And that the health functional food prepared using such a food composition can prevent or ameliorate obesity.

Accordingly, the present invention relates to a functional food composition for antiobesity and antidiabetic use which comprises, as an active ingredient, at least one extract selected from the group consisting of a herring, a herb, a green tea, a reed, a lotus flower, a pungent Chinese cabbage, a lemonade, .

The extract according to the present invention can be obtained by extracting and isolating from nature using an extraction and separation method known in the art, and the 'extract' defined in the present invention can be obtained by using a suitable solvent, For example, at least one plant selected from the group consisting of green tea, green tea, green tea, reed, lotus, pear, cabbage, ladybug, pine, fig, citron and marine oil. Extracts. Preferably, it may be an extract extracted from leaves, flowers, stems and roots of the plant.

As a suitable solvent for extracting the extract from at least one plant selected from the group consisting of the above-mentioned herd, sorghum, green tea, reed, lotus, pear, cabbage, pear, lentil, fig, citron and corn oil, The solvent may be water or an organic solvent. Examples of the solvent include, but are not limited to, purified water, methanol, ethanol, propanol, isopropanol, butanol or the like, or an alcohol having 1 to 4 carbon atoms, such as acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane ) And cyclohexane can be used alone or in combination.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation to the method for producing at least one extract selected from the group consisting of a herring, an edible plant, a green tea leaf, a reed, a lotus flower, a pale green cabbage, a lily of the valley, a lentil, a fig, an citron and a marine oil of the present invention. .

For example, one or more kinds of extracts selected from the group consisting of a herring, an edible oil, a green tea, a reed, a lotus, a pale green cabbage, a lemonade, a pepper, a fig, a citron and a marine oil contained in the composition of the present invention may be obtained by the above- May be prepared into a powder state by an additional process such as vacuum distillation and freeze-drying or spray-drying. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, one or more kinds of extracts selected from the group consisting of a herring, a herring, a green tea, a reed, a lotus flower, a pale green cabbage, a lemonade, a pimento, a fig, a citron and a marine oil are all extracted, Fractions and tablets, their diluted solutions, concentrates or dried products.

In one embodiment of the present invention, the extract of at least one herb selected from the group consisting of a herring, an edible plant, a green tea leaf, a reed, a lotus flower, a pale green cabbage, a lentil, And the extract of the present invention may be contained in an amount of 0.1 to 95% by weight based on the total weight of the composition.

The composition of the present invention may be a food composition containing the above-mentioned extract as an active ingredient. Such a food composition may be an effective ingredient such as a herring, an edible product, a green tea, a reed, a lotus flower, a pale green leaf, The composition of the present invention may contain various flavors or natural carbohydrates as additional components such as ordinary food compositions.

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. The above-described flavors can be advantageously used as natural flavorings (tau martin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.).

In addition, the food composition of the present invention may be formulated into a food composition containing at least one pharmaceutically acceptable or pharmaceutically acceptable carrier in addition to the above-described effective ingredients.

The form of the food composition may be a tablet, a capsule, a powder, a granule, a liquid, a ring, a liquid, a syrup, a juice, a suspension, an oil, or a drip agent. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural sugars such as starch, gelatin, glucose or beta-lactose, natural and synthetic gums such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, sodium Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like. Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile water and sterile water suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like.

The food composition of the present invention formulated in the above-described manner can be used as a functional food or added to various foods.

Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolates, foods, confectionery, pizza, ram noodles, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes, .

The food composition may further contain one or more kinds of nutrients, vitamins, minerals (electrolytes) other than the extracts selected from the group consisting of active ingredients such as a herring, an edible oil, a green tea, a reed, a lotus flower, , Flavorings such as synthetic flavors and natural flavors, colorants and thickeners (such as cheese and chocolate), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, Glycerin, alcohols, carbonating agents used in carbonated beverages, and the like. In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks.

The extract of at least one selected from the group consisting of the active ingredients of the present invention, such as a herring, an edible plant, a green tea, a reed, a lotus flower, a pungent Chinese cabbage, a lily of the pine, an alder, a fig, an citron and a marine oil is a natural substance, It can be used safely even when taken for a long time for the purpose of imparting obesity and / or antidiabetic function.

The present invention also relates to a health functional food for preventing or ameliorating obesity comprising at least one extract selected from the group consisting of a herring, a herring, a green tea, a reed, a lotus flower, a pungent Chinese cabbage, a lily of the pine, a lentil, a fig, to provide.

The health functional food of the present invention can be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, and rings for the purpose of preventing or improving obesity.

In the present invention, the term " health functional food " refers to a food prepared or processed using raw materials or ingredients having useful functions in accordance with the Act on Health Functional Foods, and the nutrients are controlled Physiological action, etc., for the purpose of obtaining a beneficial effect.

The health functional foods of the present invention may contain conventional food additives and, unless otherwise specified, whether or not they are suitable as food additives are classified according to the General Rules for Food Additives approved by the Food and Drug Administration, Standards and standards.

Examples of the items listed in the above-mentioned "food additives" include chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as persimmon extract, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

For example, the health functional food in the form of tablets may contain at least one extract selected from the group consisting of the active ingredients of the present invention, such as a herring, , A binder, a disintegrant, and other additives may be granulated by a conventional method, followed by compression molding with a lubricant or the like, or the mixture may be directly compression-molded. In addition, the health functional food of the tablet form may contain a mating agent or the like if necessary.

The hard capsule of the capsule form of the health functional food may be one or more selected from the group consisting of the active ingredient of the present invention such as a herring, an edible oil, a green tea, a reed, a lotus, a pale green cabbage, a sweet potato, The above-mentioned extract may be prepared by filling a mixture with an additive such as an excipient. The soft capsule may be prepared by filling a mixture obtained by mixing the extract with an additive such as an excipient into a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The ring-shaped health functional food may contain one or more kinds of extracts selected from the group consisting of the active ingredients of the present invention such as a herring, an edible plant, a green tea, a reed, a lotus flower, a pear leaves, Release and the like can be molded and formed into a mixture by a known method. If necessary, the mixture can be coated with white sugar or other skin care agent, or the surface can be coated with a substance such as starch or talc.

The granular health functional food may contain one or more extracts selected from the group consisting of the active ingredients of the present invention such as a herring, an edible oil, a green tea, a reed, a lotus flower, a pear leaves, And the like can be mixed into a granular form by a conventionally known method and, if necessary, a flavoring agent, a mating agent and the like can be contained.

The health functional food containing the extract of the present invention as an active ingredient selected from the group consisting of a herring, a herring, a green tea, a reed, a lotus flower, a pungent Chinese cabbage, a lily of the pine, a lentil, a fig, As such, it has been experimentally proved that the anti-obesity and hypoglycemic effect (antidiabetic effect) are effective to prevent or ameliorate obesity.

The health functional food may be a beverage, a meat, a chocolate, a food, a confectionery, a pizza, a ramen, a noodle, a gum, a candy, an ice cream, an alcoholic beverage, a vitamin complex and a health supplement food.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Investigation of the yield according to the solvent type

Extraction of lyophilized samples In order to investigate the recovery rate depending on the type of solvent, 5 g of each powder sample was weighed and placed in an Erlenmeyer flask and immersed in 50 ml of distilled water. The extraction was repeated three times, and the extract was filtered with a filter paper, and then completely concentrated with a vacuum concentrator, and the recovery of each sample was examined by measuring the weight. As a result, methanol and ethanol extraction showed slightly higher recovery than water extraction in most samples.

Yield by extraction solvent type Plants Yield (g) MeOH EtOH Water Salicornia herbacea 0.61 0.58 0.51 Corylopsis coreana (Stem) 0.58 0.59 0.49 Erythronium japonicum (Leaf) 0.56 0.53 0.46 Erythronium japonicum (Root) 0.63 0.65 0.52 Phragmites communis (Root) 0.52 0.49 0.42

< Example  2>

General compositional analysis

<2-1> Moisture

Chapter 10 of the FOOD STANDARDS 1. Tested according to 1.1.1 General Component Test Method 1.1.1 Moisture Content Test Method. 3 to 5 g of the specimen is precisely weighed (if it is difficult to dry, 20 g of 20 mesh refined margarine and a glass rod are put into a constant weight, Put the sticks as they are), open the lid slightly, put in a drier of the specified temperature for 3 to 5 hours, cool in a desiccator for about 30 minutes and weigh. The weighing dish is again dried for 1-2 hours and the same operation is repeated until the weight becomes constant. Generally, the water content is measured by drying for 4 hours at a time (3 hours for oil and dairy products).

a: Weight of weighing dish (g)

b: weight of weighing plate and sample (g)

c: Weight after drying (g)

<2-2> Crude protein

Food Code Article 10. General Testing Methods 1. Food ingredient test method 1.1 General component test method 1.1.3 Nitrogen compound 1.1.3.1 Total nitrogen and crude protein b. Experiments were conducted according to the method using a protein analyzer. In other words, take about 1 g of the sample (for food containing 3 to 25% protein) accurately, place it in a digestion tube, and add 2 digestion promoters. The decomposition accelerator should be decomposed efficiently when the ratio of H2SO4 to K2SO4 is 1.4 to 2.0: 1. Add 12 mL of concentrated sulfuric acid to the digestion tube. If the fat content of the specimen is 10% or more, add 15 mL of concentrated sulfuric acid. After decomposing in a decomposition apparatus at 420 ° C for 45 to 60 minutes, the decomposition liquid is cooled to room temperature when the color of the decomposition liquid becomes transparent pale blue (when using a copper catalyst) or transparent yellow (when using a selenium catalyst). Carefully add 80 mL of distilled water to the degraded test solution. Place the collection solution containing 25 mL of the mixing indicator into an Erlenmeyer flask, place it in the distillation apparatus, and raise the Erlenmeyer flask stand. During the distillation, the distillate enters the collecting solution. Add 50 mL of 40% NaOH (4 times the amount of sulfuric acid used in the decomposition) into the digestion tube. Distill in the distillation apparatus for 3 to 4 minutes. The collection solution in the Erlenmeyer flask of the distillation apparatus turns green while collecting the alkali (ammonia) contained in the distillate. The distillate is titrated with a hydrochloric acid solution (generally 0.1 N or 0.2 N) until the end point reaches a pale pink color. Record the amount of acid used in the titration.

0.0014: N amount (g) equivalent to 1 ml of 0.1 N HCl

a: Optimum consumption (ml) of 0.1N HCl in blank test

b: Proper consumption (ml) of 0.1 N HCl in this test

F: Potency of 0.1N HCl standard solution

<2-3> Crude fat

Food Code Article 10. General Testing Methods 1. Food ingredient test method 1.1 General component test method 1.1.5 Lipid 1.1.5.1 Root fat 1.1.5.1.1 Ether extraction method a. Experiments were conducted according to the general method. In other words, 2 to 10 g of the specimen (if necessary) is put into a fine powder and put into a cylindrical filter paper. The specimen is lightly filled with a cotton swab, and the cotton swab is placed in a suitable container and dried in a drier at 100 to 105 ° C. for 2 to 3 hours. And placed in the extraction tube of the Soxhlet extractor. Add approximately 1/2 volume of anhydrous ether to the receiver and extract for 8 hours. After the extraction, remove the cooler, remove the cylindrical filter paper from the extraction tube with tweezers, connect the cooler to the extraction tube again and warm in a water bath. When all of the ether in the vessel is transferred to the extraction tube, remove the receiver and remove ether Evaporate completely. Wipe the outside of the receiving bowl thoroughly with gauze, put it in a dryer at 98 ~ 100 ℃, dry it for about 1 hour until it becomes constant, then cool it in a desiccator and weigh. The amount of crude fat is calculated according to the following formula.

W ₀ : Weight of receiving bowl (g)

W ₁ : Weight (g) of the recipient vessel that has extracted the crude fat and dried.

S: Amount of sample (g)

Common components according to the type of extract Sample moisture(%) Crude protein (%) Crude fat (%) Salicornia herbacea 7.9870 13.5941 1.7550 Corylopsis coreana (Stem) 7.3372 4.0351 1.1711 Corylopsis coreana (Flower) 8.8536 13.3113 5.6246 Erythronium japonicum (Leaf) 11.8914 16.2051 5.1943 Erythronium japonicum (Root) 10.4069 4.3803 0.5960

Crude protein was higher in sorghum and green tea, and crude fat was higher in heather and sorghum leaves, but there were some differences between plant species. Moisture content was higher in the leaves and leaves than in the other leaves.

< Example  3>

Anti-diabetic  efficacy

Glucose tolerance test was performed by measuring glucose content. In other words, the effect of each sample extract on the glucose content was analyzed using BCS Glucose kit. The enzyme solution was dissolved in 1.5 ml of the enzyme solution, and 10 μl of the extract was added to the solution. The mixture was heated at 37 ° C for 5 minutes, and the absorbance at 505 nm was measured within 40 minutes. glucose content. At this time, 20 ml of the standard solution (glucose 200 mg / dl) was added to 1.5 ml of the enzyme solution as a standard solution.

Glucose content (mg / dl) = (OD _sample / OD _standard ) 200 mg / dl

The antidiabetic effect of ethanol extracts by plant type (glucose amount) Plants Glucose (mg / dl) standard (Con.) 200 ± 0.00 Salicornia herbacea 18.0 + - 0.32 Corylopsis coreana (Stem) 5.4 ± 0.12 Corylopsis coreana (Flower) 42.6 ± 0.62 Erythronium japonicum (Leaf) 50.2 ± 3.58 Erythronium japonicum (Root) 40.7 ± 1.94 Erythronium japonicum (Flower) 64.7 ± 1.43 Phragmites communis (Root) 44.6 ± 2.45 Phragmites communis (Shoot) 31.8 ± 2.53 Nelumbo nucifera (Leaf) 25.5 ± 0.63 Salvia plebeia 28.6 ± 2.63 Momordica Charantia 29.9 ± 2.31 Portulaca oleracea 7.5 ± 0.12 Ficus carica (Leaf) 29.1 ± 1.32 Citrus junos (Pericarp) 57.0 + - 2.62 Citrus junos (Leaf) 23.0 + - 1.70 Cornus officinalis 49.9 ± 4.46

* Data represent the mean values ± SE of five independent experiments.

The BCS glucose assay was used to determine the effects of 11 different plant extracts and plant extracts on glucose content. Especially, green tea, herring, and black pepper showed remarkably excellent blood glucose lowering effect, and relatively good results were also obtained in the leaves of Yeonpyeo, Gombo Chinese cabbage, Reed roots and Citron.

< Example  4>

Analysis of Fat and Carbohydrate Inhibitory Activity Anti-obesity  efficacy)

<4-1> Alpha- Amylase  Inhibitory activity

The α-amylase inhibitory activity was obtained by dissolving 5 g of agar and 5 g of soluble starch in 500 mL of distilled water and sterilizing them. 20 μl of 20 mg / ml plant extract and α-amylase were placed on a paper disc and incubated at 37 ° C. for 24 hours. I2 / KI (5 mM I2 in 3% KI ) Was added for 5 minutes. After coloring for 15 minutes, the area was measured by measuring the radius of the clear zone and calculating the inhibition rate as πr2. The positive control was acarbose and the negative control was water and methanol, which are the solvents of the extract (see FIG. 1).

The degree of suppression of alpha-amylase activity according to the type of extract plants a-Amylase Inhibitory activity (%) water MeOH Salicornia herbacea 65.3 ± 1.91 N.I. Corylopsis coreana (Stem) 34.5 + 2.54 N.I. Corylopsis coreana (Flower) 64.5 ± 2.21 N.I. Erythronium japonicum (Leaf) 38.3 ± 1.91 5.7 ± 1.14 Erythronium japonicum (Root) 51.6 ± 2.22 6.7 ± 0.09 Erythronium japonicum (Flower) 64.5 ± 1.63 4.7 ± 0.13 Phragmites communis (Root) 72.3 ± 1.25 6.2 ± 1.16 Phragmites communis (Shoot) 75.3 ± 1.18 N.I. Nelumbo nucifera (Leaf) 35.3 ± 1.13 N.I. Salvia plebeia 68.7 ± 3.27 12.8 ± 1.16 Momordica Charantia 38.3 ± 1.91 24.0 ± 3.21 Portulaca oleracea 10.9 ± 1.08 23.8 ± 2.23 Ficus carica (Leaf) 36.8 ± 2.90 24.5 + - 4.25 Citrus junos (Pericarp) 51.6 ± 2.22 44.2 ± 3.58 Citrus junos (Leaf) 28.7 ± 3.27 N.I. Cornus officinalis 62.3 ± 1.91 61.2 ± 3.18

* Data represent the mean values ± SE of three independent experiments.

NI: Not inhibited.

The inhibitory activity of α-amylase in 11 plants was found to be highest in water extracts, and the inhibitory effects of methanol extracts were higher in citron peel and corn oil. there was. However, most of the methanol extracts showed almost no inhibitory effect.

<4-2> Alpha- Glucosidase  Inhibitory activity

The inhibitory activity of α-glucosidase was determined by mixing 20 mg / mL of plant extract, 100 μL of 0.15 U / mL α-glucosidase enzyme, and 720 μL of 0.2 M potassium phosphate buffer (pH 6.8) After incubation at room temperature for 5 minutes, 100 μL of 5 mM 4-nitrophenyl-α-D-glucopyranoside (pNPG) was added and reacted at room temperature for 10 minutes. The absorbance was measured at the same wavelength. Respectively.

The degree of inhibition of alpha-glucosidase activity by the type of extract plants a-Glucosidase Inhibitory activity (%) water MeOH Salicornia herbacea 31.5 ± 4.02 N.I. Corylopsis coreana (Stem) 79.2 ± 0.38 87.4 ± 0.28 Corylopsis coreana (Flower) 87.2 ± 0.97 99.3 ± 0.69 Erythronium japonicum (Leaf) 13.6 ± 1.18 N.I. Erythronium japonicum (Root) 17.8 ± 1.39 N.I. Erythronium japonicum (Flower) 12.5 ± 1.99 N.I. Phragmites communis (Root) 39.2 ± 1.87 N.I. Phragmites communis (Shoot) 29.8 ± 2.31 15.2 ± 1.05 Nelumbo nucifera (Leaf) 34.6 ± 1.52 82.0 ± 2.59 Salvia plebeia 83.9 ± 0.93 22.6 ± 2.31 Momordica Charantia 48.8 ± 3.25 N.I. Portulaca oleracea N.I. N.I. Ficus carica (Leaf) N.I. N.I. Citrus junos (Pericarp) 19.2 ± 1.15 14.9 ± 3.42 Citrus junos (Leaf) N.I. N.I. Cornus officinalis 81.7 ± 0.14 89.5 ± 0.28

* Data represent the mean values ± SE of three independent experiments.

NI: Not inhibited.

The inhibitory activities of α-glucosidase in 11 species of plants showed that both water extracts and methanol extracts showed high inhibitory activity against the herbal stem and herring flower extracts. In addition, the methanol extracts showed a large difference in activity among plant species, and it was confirmed that the herbal extracts, herbs, leaves and mint oil had relatively high inhibitory activity.

<4-3> Pancreatic lipase  Inhibitory activity

Porcine pancreatic lipase (PPL, type II) activity was measured using p-nitrophenyl butyrate (p-NPB) as a substrate. First, the PPL stock solution was prepared to a concentration of 1 mg / mL in 0.1 mM potassium phosphate (pH 6.0), and stored at -20 ° C. To measure lipase inhibitory activity, 25 μL of extract (20 mg / mL) and 25 μL of PPL solution were added to 950 μL of 0.1 mM potassium phosphate (pH 7.2, 0.1% Tween 80) and incubated at 30 ° C. for 1 hour. NPB was added and reacted at 30 ° C for 5 minutes, and the absorbance was measured at 405 nm.

Inhibition activity (%) = [1- (B-C) / A] 100)

A: Absorbance without added sample

B: Absorbance with addition of sample

C: Absorbance without addition of enzyme

The degree of inhibition of lipase activity by the type of extract plants Lipase Inhibitory activity (%) water MeOH Salicornia herbacea 31.9 ± 1.76 43.5 ± 0.93 Corylopsis coreana (Stem) 23.4 ± 5.46 45.0 + - 4.16 Corylopsis coreana (Flower) 45.8 + 4.68 35.1 ± 2.86 Erythronium japonicum (Leaf) 19.3 ± 0.30 52.6 ± 1.05 Erythronium japonicum (Root) 8.7 ± 1.12 50.2 ± 2.96 Erythronium japonicum (Flower) 14.9 ± 3.55 59.3 ± 1.77 Phragmites communis (Root) 23.5 + 5.03 57.7 ± 1.94 Phragmites communis (Shoot) 32.5 ± 5.51 59.8 ± 3.13 Nelumbo nucifera (Leaf) 35.1 ± 2.01 52.2 + 4.21 Salvia plebeia 33.3 ± 4.74 61.9 ± 6.28 Momordica Charantia 28.8 ± 1.74 54.5 ± 2.07 Portulaca oleracea 30.2 ± 3.21 61.8 ± 3.11 Ficus carica (Leaf) 20.2 ± 1.46 60.1 ± 3.82 Citrus junos (Pericarp) 83.3 ± 5.68 75.1 ± 1.97 Citrus junos (Leaf) 46.8 ± 6.15 44.9 ± 7.13 Cornus officinalis 52.7 ± 2.80 70.7 ± 3.60

* Data represent the mean values ± SE of three independent experiments.

The inhibition activity of porcine pancreatic lipase showed a relatively high inhibitory activity in the methanol extracts than the water extracts except citrus peels. Overall, there was no significant difference between the published plant species different from the results of α-amylase and α-glucosidase inhibitory activity.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (7)

1. An anti-obesity food composition comprising, as an active ingredient, a hot-water extract of a herbal flower having amylase, glucosidase and lipase inhibitory activity,
Wherein the hot-water extract of the herbal flower is contained in an amount of 0.1 to 95% by weight based on the total weight of the composition. delete delete delete A health functional food for preventing or ameliorating obesity comprising a hydrothermal extract of a herbal flower having amylase, glucosidase and lipase inhibiting activity in an amount of 0.1 to 95% by weight based on the total weight of the composition. 6. The method of claim 5,
Wherein the extract has an effect of preventing or ameliorating obesity through amylase, glucosidase and lipase inhibitory activity. 6. The method of claim 5,
Wherein said food is selected from the group consisting of beverage, meat, chocolates, foods, confectionery, pizza, ramen, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes and health supplement foods.

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