KR20160042518A - Functional Cosmetic Water and Functional Silk Mask-Pack Comprising The Same - Google Patents

Abstract

The purpose of the present invention is to provide functional cosmetic water having excellent skin tissue stabilizing, skin metabolism promoting, moisturizing, pigmentation preventing, and anti-aging effects by stably supplying nutrients, or a natural material having excellent anti-oxidizing activity or the like, and to provide a functional silk mask pack comprising the same. The functional silk mask pack according to the present invention is manufactured by soaking a non-woven fabric made of silk having sericin removed from a cocoon or raw yarn into functional cosmetic water including purified water; sericin dissolved in the purified water; fibroin, and a vitamin tree extract.

Description

Functional cosmetic lotion and functional silk mask pack containing the same [0002]

The present invention relates to a functional lotion and a functional silk mask pack containing the functional lotion, and more particularly to a functional lotion and a functional silk mask pack containing sericin, fibroin and vitamin tree extract extracted from a silkworm cocoon.

Today, not only for women but also for men, the mask pack is used to clean and clean the skin by supplying moisture and cosmetic ingredients to the skin by wrapping the frequently exposed skin on the face, and to restore the skin physiological function.

Such a mask pack is manufactured by impregnating a nonwoven fabric made of synthetic fibers with a lotion containing various nutrients suitable for skin care, as disclosed in Japanese Patent No. 1,260,021 (hereinafter referred to as Patent Document 1) By applying it with time, the skin beauty effect is exerted.

However, in the conventional nonwoven fabric as in Patent Document 1, since the fibers are bonded with the synthetic resin adhesive, when the skin is sensitive or allergic, there is a fear of skin trouble when coming into contact with the skin, There is a problem that environmental pollution is caused

Recently, various natural raw materials such as aloe, cucumber and loess are used as the lotion used in the mask pack. These raw materials have some effects on the stabilization of skin tissue, promotion of skin metabolism, moisturization, etc. However, Ultraviolet rays, exhaust gas, toxic chemical substances, etc., skin aging and the like can not be effectively prevented.

On the other hand, in a patent No. 1,315,561 (hereinafter referred to as "Patent Document 2"), selenium-containing silkworms which are excellent in antioxidative activity and capable of exerting an excellent effect on preventing skin diseases and improving skin by removing active oxygen Discloses a silk mask pack containing an extract and a seam soluble salt.

However, in the Patent Document 2, the problem of the conventional mask pack is partially improved. However, in order to obtain the selenium-containing silkworm extract and the seam soluble salt, selenium should be added to the artificial diet fed to the silkworm, When the content of selenium supplied to the silkworm is small, it is difficult to expect the addition effect because the selenium is not transferred to the silkworm gland. When the silkworm is too much, there is a problem that the silkworms lose weight and die. There is a problem that it is difficult to obtain a melt.

In other words, it is urgent to develop a natural substance which can be supplied economically and stably while preventing skin damages such as stabilization of skin tissue, promoting skin metabolism, moisturizing, pigment change and skin aging.

Patent Document 1: Korean Patent No. 1,260,021 Patent Document 2: Korean Patent No. 1,315,561

The present invention takes the problems of the conventional mask pack as described above into consideration, and stably supplies natural substances excellent in nutritional components and antioxidative activity, thereby achieving excellent stability to skin tissue, promoting skin metabolism, moisturizing, And to provide a functional lotion and a silk mask pack containing the functional lotion.

The functional lotion according to the present invention is characterized by containing purified water and sericin dissolved in the purified water and vitamin tree extract.

The sericin of the functional lotion according to the present invention is recovered from the refining wastewater discharged in the refining process for removing sericin from the silkworm cocoons or silkworms and the vitamin tree extract is produced by drying at least one of the leaves, The resulting pulverized product was immersed in 50% ethanol mixed with water and ethanol for one week, and then filtered to remove solids. The ethanol extract solution from which the solid was removed was centrifuged and the supernatant was concentrated under reduced pressure .

The refining process for removing sericin from the silkworm cocoons or silkworms of the functional lotion according to the present invention is characterized in that the bath ratio of the silkworm cocoons or silkworms and distilled water is adjusted to 1:15 to 25 at a high temperature of 80 to 120 ° C for 1 to 3 hours A step of removing sericin from the silkworm cocoon or silkworm by refining to recover sericin from the refining wastewater comprising sericin aqueous solution is characterized in that sericin contained in the refining wastewater is hydrolyzed and then sericin is pulverized.

The hydrolysis of the sericin of the functional lotion according to the present invention is carried out by adding a single or two kinds of enzymes such as alkalase, flavozymes or protamex to the sericin aqueous solution, which is refined wastewater, The concentrated sericin solution was subjected to a freezing process in a freezing chamber of -4 ° C or below for a certain period of time and then thawed Thereby forming a powder.

The functional lotion according to the present invention further comprises fibroin powder, wherein the fibroin powder is prepared by mixing a solution of 40 g of fibroin fibers refined by removing sericin from the silkworm cocoons or silkworm, For 7 hours, and then the dissolved fibroin solution is decomposed with an alkaline protease, followed by concentration and drying to be pulverized.

The silk mask pack according to the present invention is characterized in that it is produced by impregnating a silk-woven non-woven fabric in which sericin is removed from the silkworm or silkworm, into the functional lotion.

According to the present invention, it is possible to provide functional lotion and silk mask pack containing sericin, fibroin and vitamin tree extract which are excellent in nutritional components and antioxidative activity and can be stably supplied, thereby stabilizing skin tissue, promoting skin metabolism, , Can exert excellent effects on pigment change and skin aging.

Hereinafter, specific embodiments of the present invention will be described in detail.

The functional silk mask pack according to the present invention comprises sericin, fibroin and vitamin A nut extract extracted from silkworm cocoon.

Silk from silkworm cocoons consists of two proteins, fibroin and sericin. Among them, fibroin is the fiber we call silk, and sericin is the protein that surrounds two fibroin.

Typically, sericin is removed through the refining process of silk, and after the sericin has been removed, the silk has inherent gloss and texture. Sericin accounts for about 25% of the total silk protein, , When such refining waste containing sericin is discharged into a river, it eventually causes eutrophication of the river, which causes pollution.

Therefore, many studies have been made on methods for recovering discarded sericin and utilizing it as a renewable resource. Serine has amino acid composition of about 30% and high hydrophilic amino acid content.

In particular, the amino acid composition of sericin is known to be superior to the natural moisturizing factor (NMF) of the human body, and thus has excellent skin moisturizing effect. In addition to the moisturizing effect, sericin has a sulfation effect and lipid peroxidation and tyrosinase Inhibition of skin whitening due to inhibition of activity, and inhibition of skin cancer.

Fibroin has been used as a sewing thread since ancient times because of its excellent biocompatibility and biodegradability. Fibroin powder has excellent hygroscopicity and ultraviolet shielding ability It is known to have excellent properties.

Vitamin tree (Hippohae rhamnoides L.) is a deciduous shrub widely grown in Europe, Russia, Canada, China, and Mongolia. In Korea, it is known as vitamin tree or mountain tree. And fruits. It contains more than 190 kinds of vitamins (AK E), 22 kinds of fatty acids, 42 kinds of lipids, organic acids, amino acids, vitamin C, B1, B2, Folic acid, , Phenol, tannin, and 20 kinds of carbohydrates.

The fruits of these vitamins are rich in antioxidants such as polyphenols, tocophenols, carotenoids and flavonoids, and are effective in the treatment of skin diseases, wounds, burns and inflammation. Vitamin tree leaves contain physiologically active substances such as quercetin, gallic acid and tannin, and are effective for pharmacological effects, cytotoxicity and anticancer effects. Also, flavonoid, tannin, vitamin C, protein, fat, fiber, amino acid and minerals It is said to be abundant.

[Production of nonwoven fabric of silk mask pack]

The silkworm extracted from the silkworm cocoon is a state in which sericin has not been removed, and this silkworm is rough and stiff because sericin is coated with fibroin. Therefore, sericin needs to be removed through a refining process, and after the sericin has been removed, the gloss and texture of the silk are inherent.

Therefore, the nonwoven fabric used as the sheet of the silk mask pack includes a refining step of removing sericin from the cocoon or silk, and a step of weaving the sericin-removed silk into a nonwoven fabric in the refining step.

As the refining method for removing sericin, there are various methods such as a hot water extraction method, a soap refining method, and an alkali-salt refining method. Soap scouring and alkaline-salt scouring have been widely used in the industrial field. However, the soap scouring method and the alkali-salt scouring method are based on the premise that the generated scouring wastewater is to be disposed of. In order to recover sericin therefrom, It is necessary to separate the remaining surfactant or base to be removed and it is difficult to recover pure sericin.

On the other hand, the hot water extraction method uses only distilled water at a high temperature of 80 to 120 ° C. In this case, there is an advantage that no additional process is required to recover sericin from the refining wastewater as in the case of the soap refining method by using only pure water. In addition, in the case of the hydrothermal extraction method, there is a disadvantage in that the molecular weight of the recovered sericin is lowered. However, since the sericin is removed from the silkworm or silkworms in the food and cosmetic fields, Do.

Therefore, hot water extraction method using only raw and distilled water was used as the refining method in the embodiment of the present invention. That is, the sesame seeds and the distilled water were heated at 120 ° C. for 1 hour at a ratio of 1:20 to remove all sericin from the silkworms and processed into silk composed of fibroin.

Generally, sericin is known to be hydrophilic and easy to dissolve in water, but cericin surrounding the fibroin has a layered structure that is less likely to dissolve in water from the outer layer to the inner layer.

Therefore, at a low temperature of 50 to 60 캜, only a part of the sericin in the outer layer is dissolved in water and sericin in the inner layer is not dissolved. Therefore, the bath ratio of raw silk and distilled water is changed from 1:15 to 25, It is preferred to refine for a period of time, more preferably to refine at 100 to 120 캜.

Next, the nonwoven fabric is produced using the silk from which sericin has been removed from the silkworm or silkworm. The nonwoven fabric can be produced by a conventional method, and a detailed description thereof will be omitted.

[Recovery of sericin from refining wastewater]

The process of recovering sericin from the wastewater from the refining process by the hot water extraction method can be generally summarized as a process of powdering sericin.

Generally, sericin is known as a hydrophilic protein, but sericin prepared by hydrothermal extraction is still a polymer substance, and becomes less soluble in water after it is powdered. When the solubility in water is low, there is a problem in that it is difficult to use it as a raw material contained in the lotion of the mask pack.

In order to solve the solubility problem of sericin, a low molecular weight process of sericin is required through hydrolysis.

Therefore, in the embodiment of the present invention, the recovery process of sericin from the refining wastewater is carried out through a hydrolysis step and a pulverization step of sericin.

(1) hydrolysis of sericin

There is known a method of hydrolyzing sericin under acidic or fumaric conditions by the hydrolysis method of sericin. However, in this case, an additional process of neutralization desalting is required, and sericin is hydrolyzed indiscriminately, resulting in problems in reproducibility of product performance.

Thus, in the examples of the present invention, a low molecular weight method using an enzyme was used. When enzymes are used, a specific reaction occurs, sericin molecules having a constant molecular weight can be reproducibly produced, and it can be regarded as a safer process because no harmful substances such as hydrochloric acid or sodium hydroxide are used.

Specifically, in the practice of the present invention, the hydrolysis is carried out by using an alcalase enzyme, a flavorzyme enzyme, and a protamex enzyme in the wastewater obtained in the above-mentioned refining process, that is, in an aqueous solution of sericin Respectively.

The amount of the enzyme added to the sericin aqueous solution (refining wastewater) was 10 μl of the enzyme for the alkalase, 80 mg of the flavosome enzyme and 10 mg of the protease, per 1 g of sericin, and the enzyme was hydrolyzed at 37 ° C. for 6 h .

The molecular weight of sericin hydrolyzed by hydrolysis was about 66kDa, whereas the enzyme hydrolyzed by alkalase showed the majority of sericin hydrolyzate in the range of about 14 ~ 66kDa. Especially, 34kDa And 27 kDa, respectively.

Hydrolysis with Flavobaim enzyme showed a broad molecular weight distribution and peak at 11 kDa. Hydrolysis of the protein with protease enzymes showed a molecular weight of 13-66 kDa, The 33 kDa sericin molecule appeared strong.

Therefore, it is preferable to hydrolyze the sericin aqueous solution, which is a refining wastewater, by adding one or two kinds of enzymes such as alkaline protease, flavosome enzyme or protease enzyme. When two kinds of enzymes are used, It is preferable to mix the two enzymes using 1/2.

(2) Powdering of sericin

Examples of a method for pulverizing sericin from a sericin aqueous solution, which is a refining wastewater, include a freeze-drying method, a spray drying method, and a freeze-thawing method.

The freeze-drying method is a method of drying at -40 ° C for a long period of time and then drying under vacuum. However, it has a disadvantage of high time and energy consumption. In the spray drying method, sericin solution is heated in a high- It is possible to recover a large amount of powder in a short period of time. However, it is not suitable for the powdering of sericin due to high energy and low recovery rate.

On the other hand, the freeze-thawing method is preferable in that the sericin proteins induce a large association behavior in the process of freezing and thawing, thereby allowing the precipitated sericin to be easily pulverized. .

Therefore, in the embodiment of the present invention, the sericin aqueous solution, which is the refining wastewater hydrolyzed by the enzyme, is concentrated through a separation process using a tubular ultrafiltration membrane having a cutoff molecular weight of 4,000, and then the concentrated sericin solution is cooled In a freezing chamber for a certain period of time, followed by thawing, to obtain a sericin powder.

[Preparation of fibroin powder]

First, the fibroin fiber (silk) may be obtained by removing sericin from the silkworm cocoons or silkworms through a refining process as described above, but it is preferable to use the waste produced by the defective or the filament in the silty industry.

However, in order to use such fibroin as a raw material capable of dissolving in the lotion of a mask pack, it is necessary to prepare fibroin composed of a polymer with a low molecular weight powder.

Thus, in an embodiment of the present invention, the production of fibroin powder comprises a first step of dissolving the refined fibroin fibers in water at high temperature, and a second step of dissolving the dissolved fibroin solution in the proteolytic enzyme alcalase And a second step of concentrating and drying the protein-decomposed aqueous solution.

≪ Measurement of solubility of fibroin &

To examine the solubility of fibroin in water at high temperature, fibroin was dissolved in water at a temperature of 170 캜, 180 캜, 190 캜 and 200 캜 at 4% (water 1 L: fibroin 40 g, bath ratio 1:25) The solubility was measured after dissolving for 5 or 7 hours. The results are shown in Table 1 below.

Temperature time Solubility
170 ℃
3h 10% 5h 43% 7h 60%
180 DEG C
3h 85% 5h 90% 7h 95%
190 ℃
3h 97% 5h 98% 7h 100%
200 ℃
3h 100% 5h 100% 7h 100%

As shown in Table 1, when fibroin was treated at 170 ° C for 3 hours, the solubility was low at 10%, and it was 43% and 60% solubility at 5 hours and 7 hours, respectively. But the solubility does not increase so much that it seems to be inefficient.

On the other hand, at 180 ° C, it was more than 90% when treated for more than 5 hours, and more than 97% when treated for 3 hours or more at 190 ° C, and 100% at 200 ° C for 3 hours.

≪ Measurement of molecular weight of fibroin powder &

The molecular weight of the fibroin powder prepared according to the embodiment of the present invention was measured to examine the degree of low fission. The molecular weight of the fibroin powder was as shown in Table 2 below.

In order to verify the effect of the protease, alcalase, the fibroin powder concentrated and dried only through the first step was used as a comparative example, and the fibroin powder concentrated and dried through the first and second steps As an example.

In addition, the molecular weight is shown as an average molecular weight instead of a molecular weight distribution because it is intended to examine the degree of low fission of the fibroin powder produced.

Temperature time Average molecular weight Example Comparative Example 170 ℃ 7h 10.9 kDa 13.1 kDa
180 DEG C
3h 9.0kDa 11.4 kDa 5h 7.9 kDa 8.3 kDa 7h 6.9 kDa 8.3 kDa
190 ℃
3h 6.1 kDa 7.8 kDa 5h 5.7 kDa 7.4 kDa 7h 5.3 kDa 6.7 kDa 200 ℃ 3h 5.0 kDa 6.1 kDa 5h 4.7 kDa 5.6 kDa

When the molecular weight of common fibroin is taken as 300 to 600 kDa, it can be seen that the degree of low molecular weight is large even at 170 占 폚 as shown in Table 2 above. In addition, the higher the temperature as a whole, the lower the molecular weight the longer the treatment time is.

In addition, as in the comparative example, it can be seen that even when the fibroin powder is produced through concentration and drying only through the first step, the degree of low molecular weight is large. However, in order to lower the molecular weight, , It can be seen that fibroin powder having a lower molecular weight can be produced by secondary decomposition of fibroin aqueous solution by proteolysis.

Thus, in the production of fibroin powder according to the embodiment of the present invention, a solution obtained by mixing 40 g of fibroin fibers refined with respect to 1 L of water is dissolved at 170 ° C to 200 ° C for 3 to 7 hours, and the dissolved fibroin solution is subjected to proteolysis It is preferable to decompose and concentrate and dry the enzyme alcalase.

Considering the solubility and low molecular weight of water, a solution obtained by mixing 40 g of refined fibroin with 1 L of water is dissolved at 180 to 200 캜 for 3 to hours, and then the dissolved fibroin solution is treated with proteolytic enzyme It is more preferable to decompose and concentrate and dry the alcalase.

[Production of vitamin tree extract]

As for the efficacy of the leaves and fruits of vitamin trees, it is widely known as described above. Here, only the method of extracting leaves, fruits and stems of vitamin trees according to the embodiment of the present invention will be explained.

In extracting vitamins, extracts of vitamins such as leaves, fruit or stem are cut, finely cut or ground, heated at high temperature using water as a solvent, filtered to obtain an extract solution, or ethanol or methanol A method of immersing it in a solvent and filtering it to obtain an ethanol extract solution and concentrating it under reduced pressure can be used.

However, when water is used as a solvent and heated at a high temperature, the nutrients contained in the leaves, fruits or stems of the vitamin tree may be destroyed. It is preferable to obtain the extract solution by immersing in ethanol or methanol as a solvent .

Therefore, in the embodiment of the present invention, the leaf, fruit or stem of a vitamin tree is dried, finely cut or crushed, and then immersed in 50% ethanol mixed with water and ethanol for one week and then filtered to remove the solids. The extract solution was centrifuged, and the supernatant was concentrated under reduced pressure to give a powder.

[Production of functional lotion]

The functional lotion is prepared by dissolving 0.01 to 5 parts by weight of sericin powder, 0.01 to 5 parts by weight of fibroin powder, 0.01 to 25 parts by weight of vitamin tree extract and 0.1 to 10 parts by weight of other additives relative to 60 to 90 parts by weight of purified water.

At this time, the amount of the sericin powder, fibroin powder, and vitamin tree extract dissolved in the purified water may be appropriately controlled or excluded depending on the function and use of the mask pack. For example, when emphasizing the skin moisturizing function of the mask pack, sericin powder is used. When the use of the mask pack is skin external treatment such as prevention of skin disease or improvement of skin, fibroin is used, and vitamin tree extract It is desirable to increase the specific gravity.

[Production of functional silk mask pack]

The functional silk mask pack is produced by cutting a silk nonwoven fabric which has undergone a refining step for removing sericin from a silkworm cocoon or silkworm, and cutting the silk nonwoven fabric into a sheet member to impregnate the sheet member with the functional lotion.

Claims (7)

Characterized in that it contains purified water and sericin and vitamin tree extract dissolved in said purified water. The method according to claim 1,
The sericin is recovered from the refining wastewater discharged in the refining process for removing sericin from the silkworm cocoon or raw silkworm,
The vitamin tree extract is prepared by drying at least one of leaves, fruits or stems of a vitamin tree, pulverizing the powder, and immersing the pulverized material in 50% ethanol mixed with water and ethanol for one week, Characterized in that the ethanol extract solution from which the solids have been removed is centrifuged and the supernatant is concentrated under reduced pressure. 3. The method of claim 2,
In the refining process for removing sericin from the silkworm cocoons or silkworms, refining the silkworm cocoons or silkworms and distilled water at a high temperature of 80 to 120 ° C for 1 to 3 hours at a bath ratio of 1:15 to 25, To remove sericin,
Wherein the step of recovering sericin from the refining wastewater comprising an aqueous sericin solution comprises hydrolyzing sericin contained in the refining wastewater and then sericin is pulverized. 4. The method of claim 3,
The hydrolysis of the sericin is carried out by adding a single or two kinds of enzymes to the sericin aqueous solution, which is the refining wastewater, such as alkalase,
The saccharin aqueous solution was subjected to a separation process using a tubular ultrafiltration membrane having a cutoff molecular weight of 4,000 and concentrated, followed by concentration of the sericin solution in a freezing chamber below -4 ° C Characterized in that it is subjected to a freezing process for a certain period of time and then thawed and pulverized. 5. The method according to any one of claims 1 to 4,
Further comprising fibroin powder,
The fibroin powder is prepared by dissolving a solution of 40 g of refined fibroin fibers removed from the silkworm cocoons or silkworms at a temperature of 170 to 200 ° C for 1 to 3 liters of water for 3 to 7 hours and then dissolving the dissolved fibroin solution Characterized in that it is decomposed with an enzyme alkalase and concentrated and dried to be powdered. A functional silk mask pack characterized by being impregnated with the functional lotion according to any one of claims 1 to 4, wherein the silk nonwoven fabric in which sericin has been removed from the cocoon or the silkworm is impregnated with the functional lotion. Characterized in that the functional silk mask pack is produced by impregnating a silk nonwoven fabric from which silkworms have been removed from a silkworm cocoon or silkworm, with the functional lotion of claim 5.