KR20160041082A - Promoter for Pollen-Specific Expression in Plants and Use Thereof - Google Patents

Abstract

Provided are a promoter exhibiting anther-specific expression characteristics in plants, a vector comprising the same, a plant transformed by the vector, and a method for transforming traits of plants using the same. The anther-specific expression promoter of the present invention can be applied in the production of transgenic crops which functionally alters anther-producing substance and existing seeds, pollination and fertilization steps in a high efficiency for increase yield of agriculturally and industrially effective crops. In addition, the anther-specific expression promoter is an anther-specific regulatory region and is capable of regulating expression of the anther-specific gene in the whole steps of the pollination and the fertilization. Thus, the promoter of the present invention can be useful in the development of genetically modified plants into which exotic genes are introduced requiring anther-specific expression.

Description

[0001] The present invention relates to a promoter having an anther expression-specific expression characteristic of a plant and a use thereof.

The present invention relates to a promoter having an anther expression-specific expression characteristic of a plant and a use thereof.

The energy depletion, the environmental pollution and the sudden change of climate caused by the use of fossil fuels after the Industrial Revolution in the last decade have called for the development of environmentally friendly new and renewable energy. In response to these demands, the development of biomass energy (bio-energy), which is produced by consuming organic matter (mainly plants) and organic matter generated by using solar energy, is getting attention, and commercialization technology development and distribution are increasing. In particular, the EU announced that it will supply more than 70% of total alternative energy to bioenergy by 2010. China will use biomass gasification products from farmers, Sweden, Canada and Brazil, Research on bio-energy is actively underway with worldwide interest.

The amount of biomass produced on the earth for about a year is large enough to match the total amount of current oil reserves, and if properly used, it can solve the problem of energy exhaustion. However, due to the recent rapid economic growth of major economies such as China and India, grain demand, supply-demand anxiety, and agrification due to the continuous consumption of bio-energy development materials, It is urgent to develop and disseminate bio-energy raw materials.

The world population is expected to grow to over one billion in the next 12 years, reaching 8.3 billion by 2030. Moreover, with the advancement of medical technology, the era of healthy 100 years old and above is expected to take place in the near future. Crop yields will gradually decrease due to the activities of civilization and global warming caused by the use of petroleum resources. Therefore, as traditional cultivation and breeding techniques can not supply the food of explosively expanding populations, it is imperative to research and develop diverse biotechnological methods to develop crops with improved resistance to disasters and biomass and yield.

Plant, which is the main raw material for producing biomass, is essential in its life history, accompanied by moisture and fertilization process through anther. Anther is formed in the pollen chamber of the flower organ, and the mature anther is exposed to the outside of the pollen chamber and stays in the stigma. Afterwards, a pollen tube is formed in the head of the stigma, and it is moved to the ovary in the stigma. The two spermatocytes transferred through the pollen tube are combined with the oocyte and the central cell in the ovary to complete the modification process. This process has a direct impact on fruit production, which is the basis for the next generation.

Korean Patent Laid-open Publication No. 10-2010-0081806 discloses a pollen-specific gene promoter pPLim3, an expression vector containing the pollen-specific promoter pPLim3, and a transformant using the expression vector. Korean Patent Publication No. 10-2005-0041087 discloses a pollen- Korean Patent Laid-Open No. 10-2006-0087960 discloses a gene specific to an apple-derived drug, a promoter thereof, and a method for producing a transgenic plant using the same. However, as in the present invention, LBD10 An anther expression-specific expression method using a promoter of a gene has not been disclosed.

Numerous papers and patent documents are referenced and cited throughout this specification. The disclosures of the cited papers and patent documents are incorporated herein by reference in their entirety to better understand the state of the art to which the present invention pertains and the content of the present invention.

The present inventors have searched for a promoter having a function to specifically express a gene in anther on an anther. As a result, Pro _LBD10 : EGFP: GUS vector containing the promoter region of LBD10 gene and EGFP and GUS gene was produced, and EGFP and GUS activities were _{observed in} the anther of the plant transformed with this vector. In other tissues except the anther The present inventors have completed the present invention by experimentally confirming that no activity occurs at all.

Accordingly, an object of the present invention is to provide a promoter of anther expression-specific expression characteristics of a plant.

It is still another object of the present invention to provide a vector comprising the promoter and the foreign gene of the present invention.

It is another object of the present invention to provide a transgenic plant transformed by the vector of the present invention.

It is yet another object of the present invention to provide a method for transforming a plant by the vector of the present invention.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, there is provided a promoter of an anther-specific expression characteristic of a plant consisting of the nucleotide sequence set forth in SEQ ID NO: 1.

The present inventors have searched for a promoter having a function to specifically express a gene in anther on an anther. As a result, Pro _LBD10 : EGFP: GUS vector containing the promoter region of LBD10 gene and EGFP and GUS gene was produced, and EGFP and GUS activities were _{observed in} the anther of the plant transformed with this vector. In other tissues except the anther It was experimentally confirmed that no activity was observed at all.

As used herein, the term " specific " means that the expression activity of a promoter is higher in a particular tissue than in at least one other tissue in the same plant body. The level of expression activity of the promoter is assessed by comparing the expression level of the promoter in tissues previously measured using methods commonly used with those in other tissues. Generally, the level of expression of a promoter is measured by the amount of gene product expressed under the control of the promoter, for example, protein and RNA.

As used herein, the term " promoter " means a DNA sequence capable of regulating the expression of a coding sequence or functional RNA. The promoter region can be easily recognized by those skilled in the art. That is, the start codon of the estimate including the ATG motif is confirmed, and the upstream is the estimated promoter region from this start codon. A promoter consists of contiguous and remote upstream elements. Adjacent elements typically include a TATA box that allows RNA polymerase II to initiate synthesis of RNA at suitable transcription initiation sites. The distant element contains a regulatory sequence, also called an enhancer, which is an additional regulatory element involved in tissue-specific or time-specific expression in the upstream region of the TATA box. An enhancer is a DNA sequence capable of promoting the activity of a promoter and may be a unique element of the promoter or a heterologous element inserted to enhance the tissue-specificity or expression level of the promoter. The promoter may be entirely derived from the original gene, or may be composed of different elements derived from different promoters found in nature, or may even contain synthetic DNA. It will be understood by those skilled in the art that each different promoter may direct expression of the gene in different tissue or cell types, or at different stages of development, or in response to different environmental conditions. A promoter that expresses a gene for most of the time in most cell types is called a " constitutive promoter ". Various types of new promoters useful in plant cells have been continuously discovered; A number of examples of promoters are disclosed in a compilation by Okamuro and Goldberg (1989, Biochemistry of Plants 15: 1-82). It is recognized that, in most cases, the exact border of the regulatory sequence is not completely defined, so that certain mutated DNA fragments have the same promoter activity.

According to another aspect of the present invention there is provided a vector comprising a promoter consisting of the nucleotide sequence described in the first sequence of the sequence listing and an exogenous gene operatively linked to the promoter.

As used herein, the term " operably linked " means a functional linkage between a nucleic acid expression control sequence (e.g., an array of promoter, signal sequence, or transcription factor binding site) and another nucleic acid sequence, The sequence controls the transcription and / or translation of the other nucleic acid sequences.

The term " foreign gene of interest " as used herein is not limited to a specific gene but can be selected according to the desired application or phenotype to be achieved.

The vectors in the present invention can be constructed through various methods known in the art, and specific methods are described in Sambrook et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press (2001) This document is incorporated herein by reference.

The vector of the present invention can typically be constructed as a vector for cloning or expression. In addition, the vector of the present invention can be constructed by using prokaryotic cells or eukaryotic cells as hosts. For example, when the vector of the present invention is an expression vector and a prokaryotic cell is used as a host, a strong promoter capable of promoting transcription (e.g., pL? Promoter, trp promoter, lac promoter, T7 promoter, tac promoter, etc.) It is common to include a ribosome binding site and a transcription / translation termination sequence for initiation of translation. When E. coli is used as a host cell, the promoter and operator site of the E. coli tryptophan biosynthetic pathway (Yanofsky, C., J. Bacteriol., 158: 1018-1024 (1984)) and the left promoter of phage l Promoter, Herskowitz, I. and Hagen, D., Ann. Rev. Genet., 14: 399-445 (1980)).

The vectors that can be used in the present invention include plasmids such as pSC101, ColE1, pBR322, pUC8 / 9, pHC79, pGEX series, pET series and pUC19 which are frequently used in the art, phages such as λgt4 · λB ,? -charon,?? z1, and M13), or a virus (e.g., SV40, etc.).

On the other hand, when the vector of the present invention is an expression vector and a eukaryotic cell is used as a host, a promoter derived from a genome of a mammalian cell (for example, a metallothionein promoter) or a mammalian virus Virus late promoter, vaccinia virus 7.5K promoter, SV40 promoter, cytomegalovirus promoter, and tk promoter of HSV) can be used, and generally have a polyadenylation sequence as a transcription termination sequence.

The vector of the present invention may be a selection marker and may include an antibiotic resistance gene commonly used in the art, for example, ampicillin, gentamycin, carbenicillin, chloramphenicol, streptomycin, kanamycin, And resistance genes for tetracycline.

According to a preferred embodiment of the present invention, the promoter suitable for the present invention may be any of those conventionally used in the art for transgenic introduction of plants, for example, a ubiquitin promoter of corn, a corynebacterium mosaic bar (CaMV) 35S promoter, nopaline synthase (nos) promoter, Piguet mosaic virus 35S promoter, water cranes Vasyl formum virus promoter, combellinella yellowmott virus promoter, ribulose-1,5-bis-phosphate carboxamide (TPI) promoter, adenine phosphoribosyl transferase (APRT) promoter of Arabidopsis, and the tryptophan synthase promoter of ssRUBISCO, a cysteine triphosphate isomerase (TPI) promoter of Arabidopsis thaliana, do.

According to one embodiment of the present invention, the 3'-non-detoxified site causing polyadenylation in accordance with the present invention is derived from the nopaline synthase gene of Agrobacterium tumefaciens (nos 3 'end) ( (Bevan et al., Nucleic Acids Research, 11 (2): 369-385 (1983)), derived from the tryptophan synthase gene of Agrobacterium tumefaciens, the protease inhibitor I or II gene of tomato or potato A 3'-terminal portion, or a CaMV 35S terminator.

Alternatively, the vector of the invention additionally carries a gene encoding a reporter molecule (e.g., luciferase and beta -glucuronidase). In addition, the vector of the present invention can be used as a selection marker for a gene resistant to antibiotics (e.g., neomycin, carbenicillin, kanamycin, spectinomycin, hygromycin) (for example, neomycin phosphotransferase (nptII) Gomycin phosphotransferase (hpt), etc.).

According to another aspect of the present invention there is provided a transgenic plant transformed by said vector.

According to another aspect of the present invention, there is provided a method for transforming a plant, which comprises transforming a plant with the vector and expressing the foreign gene in a plant.

Host cells capable of continuously cloning and expressing the vector of the present invention in a stable manner can be used in any host cell known in the art, such as E. coli JM109, E. coli BL21, E. coli RR1, E. coli . such as E. coli LE392, E. coli B, E. coli X 1776, E. coli W3110, Bacillus subtilis, Bacillus strains, and Salmonella typhimurium, Serratia marcesensus and various Pseudomonas spp. Enterobacteriaceae and strains.

When the vector of the present invention is transformed into eukaryotic cells, Saccharomyce cerevisiae, insect cells, human cells (e.g., Chinese hamster ovary, W138, BHK, COS-7, 293 , HepG2, 3T3, RIN and MDCK cell lines) and plant cells. On the other hand, since the vector of the present invention is highly useful in plants, the transformant includes not only cells but also calluses derived from plant cells or tissues.

The method of delivering the vector of the present invention into a host cell may be carried out by the CaCl ₂ method (Cohen, SN et al., Proc. Natl. Acac. Sci. USA, 9: 2110-2114 (1973) , Hanahan, D., J. MoI. Biol., 166: 557-580 (1997)), one method (Cohen, SN et al., Proc. Natl. Acac. Sci. USA, 9: 2110-2114 (1983)) and electroporation (Dower, WJ et al., Nucleic. Acids Res., 16: 6127-6145 (1988)). In addition, when the host cell is a eukaryotic cell, microinjection (Capecchi, MR, Cell, 22: 479 (1980)), calcium phosphate precipitation (Graham, FL et al., Virology, 52: 456 (Wong, TK et al., Gene, 10: 87 (1980)), DEAE- (Yang et al., Proc. Natl. Acad. Sci., 87: 9568-9572 (1990)) and dextran treatment (Gopal, Mol. Cell Biol., 5: 1188-1190 ) Or the like into the host cell.

In the method of the present invention, transformation of plant cells can be carried out according to conventional methods known in the art, including electroporation (Neumann, E. et al., EMBO J., 1: 841 (1982) ), Particle bombardment (Yang et al., Proc. Natl. Acad. Sci., 87: 9568-9572 (1990)) and Agrobacterium-mediated transformation (U.S. Patent Nos. 5,004,863, 5,349,124 and 5,416,011) . Of these, Agrobacterium-mediated transformation is most preferred.

Methods for transforming dicotyledonous plants with Agrobacterium tumefaciens and obtaining transformed plants are known, and methods for obtaining transgenic plants for a variety of other plants are known, including cotton U.S. Patent Nos. 5,004,863 and 5,159,135; See U.S. Patent Nos. 5,569,834 and 5,416011 for soybeans; For Brassica, see U.S. Patent No. 5,463,174; For peanut, see Cheng et al. (1996) Plant Cell Rep. 15: 653-657, McKently et al. (1995) Plant Cell Rep. 14: 699-703); For papaya, see Ling, K. et al. (1991) Bio / technology 9: 752-758); and pea (Grant et al. (1995) Plant Cell Rep. 15: 254-258.

Selection of transformed plant cells can be carried out by exposing the transformed culture to a selection agent (e.g., metabolic inhibitor, antibiotic, and herbicide). Plant cells that stably contain a marker gene that is transformed and conferring selectative resistance are grown and divided in the above cultures. Exemplary labels include, but are not limited to, hygromycin phosphotransferase gene, glycophosphate tolerance gene and neomycin phosphotransferase (nptII) system.

Methods for the development or regeneration of plants from plant protoplasts or from various plants are well known in the art. The development or regeneration of plants containing foreign genes introduced by Agrobacterium can be accomplished according to methods known in the art (U.S. Pat. Nos. 5,004,863, 5,349,124 and 5,416,011).

In the present invention, the preferred transformation method is carried out using an Agrobacterium system, more preferably Agrobacterium tumefaciens-binary vector system.

Transformation of plant cells is carried out with Agrobacterium tumefaciens containing Ti plasmids (Depicker, A. et al., Plant cell transformation by Agrobacterium plasmids. In Genetic Engineering of Plants, Plenum Press, New York (1983) . More preferably, binary vector systems such as pBin19, pRD400, pRD320, pGA1611 and pGA1991 are used (An, G. et al., Binary vectors "In Plant Gene Res. Manual, Martinus Nijhoff Publisher, An et al., 1988; and Lee et al., 1999).

Binary vectors suitable for the present invention include (i) a promoter that is operative in plants; (Ii) a structural gene operably linked to the promoter; And (iii) a polyadenylation signal sequence. Alternatively, the vector additionally carries a gene encoding a reporter molecule (e.g., luciferase and glucuronidase). Examples of promoters used in the binary vector include, but are not limited to, CaMV 35S promoter, 1 promoter, 2 promoter and nopaline synthase (nos) promoter.

Infection of an explant by Agrobacterium tumefaciens comprises methods known in the art. Most preferably, the infection process comprises co-culturing an explant in a culture of Agrobacterium tumefaciens with an explant. Whereby Agrobacterium tumefaciens is infected into plants.

Explants transformed by Agrobacterium tumefaciens are regenerated in regeneration medium, which ultimately forms transgenic plants.

The transformed plants according to the present invention are confirmed to be transformed by methods known in the art. For example, PCR using DNA samples from tissues of transformed plants can identify foreign genes inserted into the genome of the transgenic plant. Alternatively, Northern or Southern blotting can be performed to confirm the transformation (Maniatis et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).

The features and advantages of the present invention are summarized as follows:

(I) The present invention provides a promoter of an anther expression-specific expression characteristic of a plant and a vector containing the same, a plant transformed with the vector, and a method of transforming a plant using the vector.

(Ii) The anther-specific expression promoter of the present invention can be used for the production of transgenic crops that functionally transform production materials of existing seeds and anther, as well as high-efficiency water, fertilizer and water for increasing the yield of agricultural and industrial useful crops have.

(Iii) The anther-specific expression promoter of the present invention is an anther-specific regulatory region, which is capable of regulating an anther gene-specific gene expression at the stage of moisture, before the fertilization process, as a transgenic plant in which a foreign gene requiring a flower- It can be useful for development.

Fig. 1 is a schematic diagram of a vector used in the production of Pro _LBD10 : EGFP: GUS .
Fig. 2 shows the expression patterns of EGFP and GUS, which are reporter genes, during the development of an anther of a transgenic plant including the promoter of LBD10 gene used in the present invention. A, inflorescence; B, flowering; C and D, surgery at the flowering stage; E, polar single cell stage anthers; F, early double cell stage anthers; G, mid - late double cell stage anthers; H, triple cell stage anthers; I, mature, triple cell stage anther.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood by those skilled in the art that these embodiments are only for describing the present invention in more detail and that the scope of the present invention is not limited by these embodiments in accordance with the gist of the present invention .

Example

Example 1: Pro _LBD10 : EGFP: GUS Analysis of tissue-specific expression patterns of transgenic plants

In order to investigate the tissue in which the promoter region of the LBD10 gene was activated, a recombinant plant expression vector containing an EGFP gene encoding a green fluorescent protein as a reporter gene and an enzyme protein GUS gene encoding a blue color in the subsequent staining of an X-Glu reagent Pro _LBD10 : EGFP: GUS was constructed (Fig. 1) and transformed into Arabidopsis Columbia-0 (Col-0).

The LBD10 promoter region corresponding to about 1808 base sequences from the beginning of ATG translation of the reporter gene was ligated with Pfu DNA polymerase and two primers, LBD10proF (5'-CAC CGT CTT GAT CAT AAT GTC TTA AAA TCA C-3 ) And LBD10proR (5'-CCC CAC TTA AGA CAC AAC ACT ATC-3 '). Then, recombination reaction was performed with the pENTR ^TM / SD / D-TOPO (Invitrogen) vector using the GatewayBP recombination reaction. As a result, pENTR ^TM / SD / D-TOPO _LBD10 . The recombinant vector was constructed and recombined into the pKGWFS7 target vector using the GatewayLR recombination reaction. As a result, Pro _LBD10 : EGFP: GUS was constructed as a recombinant plant expression vector. To express this in plants, Agrobacterium was transformed into Arabidopsis thaliana using the floral dip method. After the seeds were transplanted, individual individuals growing on a 0.5X Murashige Skoog (MS), 10 mg / ml bsata medium were selected, transferred to the soil, and cultivated. The seeds were separately harvested and planted in the same composition medium The surviving and dead phenotype were identified in 3: 1 ratio and ultimately, 24 homozygous transgenic plants were isolated. Among them, the transgenic plants to be used for the experiment were grown in pots for 6 weeks.

To investigate the fluorescence expression of EGFP reporter gene-based proteins, the developing anther was isolated from the developing surgery in the bud using a needle. In addition, mature anther was separated from the flowering in the operation of ten steps. After that, nuclei in anther were stained with DAPI (4 ', 6-diamidino-2-phenylindole) reagent to confirm the development stage of anther. Samples were observed at 1000X magnification using a Leica DM2500 microscope.

In order to examine the expression of gene-derived proteins from GUS reporter, transgenic plants were cut with scissors and reacted with X-Gluc reagent at 37 ° C for 16 hours, and chloroplasts were removed using ethanol. Samples were observed at 50-1000X magnification with differential interference contrast (DIC) using a Leica DM2500 microscope.

Through the analysis of EGFP and GUS protein expression from the reporter gene, it was found that the promoter region of LBD10 gene was active throughout polar development stages from the polar single cell stage to the mature triple cell stage. In addition, no activity was observed in other tissues of plants other than anther. Through these observations, the promoter of the LBD10 gene was confirmed to be a useful promoter as an anther-specific expression promoter (Fig. 2).

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

<110> INDUSTRY-ACADEMIC COOPERATION FOUNDATION, CHONNAM UNIVERSITY <120> Promoter for Pollen-Specific Expression in Plants and Use Thereof <130> PN140476 <160> 3 <170> Kopatentin 2.0 <210> 1 <211> 1828 <212> DNA <213> LBD10 promoter <400> 1 gtcttgatca taatgtctta aaatcacaaa acttatataa aactataaaa catatattat 60 aatatataat tataatcata agttacaagt atcaatgtat taaaaaaata aatagtatca 120 atactaaata agattttttt tagttttctt aaagatattt atcaaccatt caagttaaat 180 tcaagacata atattcttgt gttattatca actaaatctt gactcttaat attgtatcaa 240 gttttgtaaa tcaataaagt atttagagaa tttaaagtaa atgttcttga ttttatttca 300 tcaagtttaa atcaatgaaa agtagttagg aacgttgatt tatcttcttt actctttcaa 360 ttttatactt ttataatgct attattggag gcaaaaaata gagaggaaga ataagctatt 420 ctctagtatc tatatgaact cttcatgtaa attactaaag aatattagtt acaagaatga 480 aatatctata acccgttcaa gttaaatttt ctatgtttca gttgtcaaac ttctgcatta 540 tcaggttgta ccttcactga atacattata ttgtagaagt ttaaatcaat gaaatattta 600 cagactctaa tattttttat attaaaactc taatacactt ttgttatcat taattaatgt 660 tttactaatt acattgtatc atattgaatc aatgatgtat tagagacagt aaaaatacta 720 ttcgtaacta gtaatgaatt atacttttca aatgatcctt ttatataaat ttcacaaatt 780 ttaaatgcta atttctcatt ctttatttga agagttcttg taaaaaatgc taaagaatgg 840 ggtatattat ttttttagat tctaaatttg aaagttaaaa attcaagacc tagtaaatca 900 gttaaaaata taaaagaaat atctaaaaaat aactcacttt ttctccattg taaacaaatt 960 tgattttctt ttcctatgaa atttgaatta gtcttctact aattatgcag cttaaaatat 1020 cattgctaac ttatcaaatg atacttcaaa ttttttatca gtcacaaaaa aaaaaacaat 1080 ttgcataagc ggtttagatt taaatttgac cacacgtgtg taactttgta acacgatgtc 1140 tctatctttc tctctttctt gttgatgtgt tttaaggttt tctaaatctc atttttgtct 1200 ccttaaaatt ttctcagaaa catttccgtc atttgttcct tagttttgta atcaatcttc 1260 acgattcctc aggcactgta cttggcaaat ctctgtcttt ttttctcctt cattttctct 1320 cactctctcc caaaaatcca gtaattctat attattttag gtgatcattc tgatttcaat 1380 ttttttggtt gaatttttct aaatagtaat gttatttcag gtaccatcct ccaaggtcat 1440 aagagaacag gatcggtgtt ctcgaacgag cgggtgcgat ttcttgattc tttaatttta 1500 tttgatcaca tttcttaatt ctctcatata tatagattga ttgatttggt tgaaaattga 1560 tttaatttca taactatgtt ttctttttca taattaaatt ttcatagttc tgtttctttt 1620 ttgttgttga gaaagttgat ctaatgtggt tgattcaaag ggtgatgtaa atgttcttgc 1680 ctggtttttg gtttcttgtg ttgaaagcta aaacgtatat gattttgtgt ttcttgtgct 1740 tcaagatatt tcttacatgt tatttccctt gttctctttg tcttaattcc attcttaaat 1800 ttttgatagt gttgtgtctt aagtgggg 1828 <210> 2 <211> 31 <212> DNA <213> LBD10proF <400> 2 caccgtcttg atcataatgt cttaaaatca c 31 <210> 3 <211> 24 <212> DNA <213> LBD10proR <400> 3 ccccacttaa gacacaacac tatc 24

Claims (5)

A promoter of an anther-specific expression characteristic of a plant consisting of the nucleotide sequence set forth in SEQ ID No. 1.
A vector comprising the promoter of anther-specific expression characteristics of the plant of claim 1.
3. The vector of claim 2, wherein the vector comprises an exogenous gene operatively linked to the promoter.
A transformed plant transformed with the vector of claim 2 or 3.
A method for transforming a plant, comprising transforming a plant with the expression vector of claim 3 and expressing the foreign gene in a plant.

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