KR20160037718A - Vehicle for inspecting circulating tumor cells in blood - Google Patents

Abstract

The present invention relates to a vehicle for inspecting blood cells, which can increase the stability of an inspection of a target cell. According to an embodiment of the present invention, the vehicle for inspecting blood cells comprises a storage unit, an inspection apparatus, and a fuel cell, wherein the inside of the storage unit is formed with an operation space. The inspection apparatus is installed in the operation space, separates the target cell in a specimen, and stores the separated target cell in a state of live cells. In addition, the fuel cell supplies a driving voltage to the inspection apparatus.

Description

VEHICLE FOR INSPECTING CIRCULATING TUMOR CELLS IN BLOOD [0002]

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a blood cell test vehicle, and more particularly, to a blood cell test vehicle capable of enhancing the stability of a test cell.

Tissue biopsy is generally performed to clinically confirm the current status of patients with some cancers, such as pancreatic cancer, lung cancer, and colorectal cancer. However, such a biopsy is difficult to repeatedly performed depending on time, cost, and condition of a patient, and there is a disadvantage that side effects may cause infection or bleeding.

Circulating Tumor Cells (CTCs) are a primary tumor tissue, a small number of tumor cells that move away from a primary carcinoma and circulate in the blood. According to statistics, about 90% of cancer patients are known to die from metastatic cancer.

Blood tumor cells range from one to several thousand per ml of blood, depending on the type of cancer, and recent advances in technology have made it possible to effectively isolate blood tumor cells.

Test apparatus for examining target cells such as blood tumor cells or rare cells are usually installed in buildings such as laboratories or medical institutions because they may be affected by minute vibrations. Further, since the internal environment such as the temperature and humidity of the space in which the inspection apparatus is installed is also important, a device for adjusting and maintaining such internal environment is installed.

On the other hand, since the inspection apparatus is installed in a fixed place such as a hospital or a laboratory, in order to collect a specimen such as blood (Clinical Specimen), it becomes troublesome for the examinee to directly visit the facility. Especially, when many people such as workplaces, government offices, and schools are required to be inspected, the cost and time consuming due to the movement of a large number of personnel can be a burden.

Therefore, an attempt is being made to provide a means of transport, such as a vehicle, so that the subject may be able to collect the specimen, isolate and inspect the target cell without having to visit a predetermined location such as a medical institution. There is also a need for an effective installation technique of the devices necessary to ensure that such inspection processes can be carried out stably and safely in vehicles such as vehicles.

In order to solve the above problems, the present invention provides a blood cell test vehicle capable of enhancing the stability of the test of target cells.

According to an aspect of the present invention, An inspection device installed in the work space for separating the target cells from the specimen and storing the separated target cells in a live cell state; And a fuel cell for supplying a driving voltage to the testing device.

In one embodiment of the present invention, the accommodating portion may further include an environment maintaining device for receiving a driving voltage from the fuel cell and maintaining an internal environmental condition of the work space.

In one embodiment of the present invention, the internal environmental conditions may include temperature and humidity.

In one embodiment of the present invention, the accommodating portion may further include an air conditioning device for receiving driving voltage from the fuel cell, circulating air in the working space, and discharging contaminants.

According to an embodiment of the present invention, a driving voltage of an inspection apparatus, an environment maintaining apparatus, and an air conditioner provided in the inspection vehicle may be supplied through the fuel cell. Since the fuel cell can supply the driving voltage in a state in which the vehicle is not started, the occurrence of vibration on the vehicle can be effectively eliminated. As described above, since the inspection vehicle can be provided with an environment free from vibration, the inspection process in the inspection vehicle can be performed stably and the accuracy of the inspection can be confirmed.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

1 is an exemplary view showing a blood cell inspection vehicle according to an embodiment of the present invention.
2 is a block diagram showing a blood cell inspection vehicle according to an embodiment of the present invention.
3 is a block diagram illustrating an apparatus for inspecting a blood cell test vehicle according to an embodiment of the present invention.
FIG. 4 is a perspective view illustrating a separation kit used in a separation process of target cells in a blood cell test vehicle according to an embodiment of the present invention. FIG.
FIG. 5 is an exploded perspective view showing a separation kit used in a process of separating target cells in a blood cell test vehicle according to an embodiment of the present invention. FIG.
FIG. 6 is a flowchart of a blood cell separation process using a separation kit used in a target cell separation process in a blood cell test vehicle according to an embodiment of the present invention.
FIG. 7 is a view illustrating a process of separating blood cells using a separation kit used in a target cell separation process in a blood cell test vehicle according to an embodiment of the present invention. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" to another part, it includes not only "directly connected" but also "indirectly connected" . Also, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is an exemplary view showing a blood cell testing vehicle according to an embodiment of the present invention. FIG. 2 is a configuration diagram showing a blood cell testing vehicle according to an embodiment of the present invention, and FIG. FIG. 2 is a configuration diagram showing an inspection apparatus of a blood cell test vehicle according to an example.

1 to 3, the blood cell testing vehicle 100 according to an embodiment of the present invention may include a receiving unit 110, a testing device 200, and a fuel cell 300.

The accommodating portion 110 may be provided in the inspection vehicle 100 and the work space 111 may be formed in the accommodating portion 110. In FIG. 1, the inspection vehicle 100 is shown as a van, but this is for illustrative purposes only, and the inspection vehicle 100 is not necessarily limited to a van.

The receiving portion 110 may be formed integrally with the inspection vehicle 100 or may be manufactured separately from the inspection vehicle 100 and then coupled to the inspection vehicle 100. That is, the receiving portion 110 can be interpreted to mean a vehicle that is connected to the inspection vehicle without power or a container (container) having a space therein. For example, the receptacle 110 may be interpreted to include a trailer or a container used to transport a cargo, wherein the workspace 111 may include a trailer or an inner space of the container Lt; / RTI >

The inspection apparatus 200 may be installed in the work space 111 of the storage unit 110 and may separate and analyze target cells from a specimen. Here, the specimen refers to the material necessary for the inspection, and may be the inspection material. Clinically, blood, fluid, pleural fluid, ascites, joint fluid, pus, secretion fluid, feces, pharyngeal mucus, urine, bile, feces and the like can be used as a specimen. In the present invention, have. Also, in the present invention, the target cell may be CTCs (Circulating Tumor Cells) and may include rare cells.

The testing device 200 may include a separation unit 210 capable of separating target cells from blood and a storage unit 250 for storing separated target cells. In addition, the testing device 200 may further comprise an inspection unit 260 for testing the target cells separated from the blood. The testing device 200 can separate the target cells from the specimen and can keep the separated target cells in a viable state.

The specimen can be received and stored after being collected from the subject, and then transferred to the inspection apparatus 200 for the target cell separation process.

Separation unit 210 is the centrifuge 211, a rotator (Rotator) (212), cell centrifuge (Cytospin) (213), cell counter (Cell Counter) (214), carbon dioxide (CO ₂₎ incubator 215 and And a clean bench (216).

Centrifuge 211 can be used to perform Ficoll Gradient Centrifugation and can also be used to recover a Peripheral Blood Mononucleated Cell (PBMC) layer.

Then, the rotator 212 can be used to mix and stir reagents in the blood.

In addition, the cell centrifuge 213 can be used for concentrating the cells contained in the liquid specimen by finely spreading the cells on a limited area of a slide glass using centrifugal force.

The cell counter 214 may then be used to count cells. The cell counter 214 may be an automatic cell counter.

In addition, the carbon dioxide incubator 215 can be used to house and culture cells therein.

In the clean bench 216, the manual work of the operator can be performed.

The separating unit 210 is a process of separating the blood from the blood by using the above-described Ficoll gradient centrifugation and peripheral blood mononuclear cell layer recovery process, chip filtration, immunomagnetic separation (IMS), and cell centrifuge (Cytospin) Of the tumor cells in the blood.

The separation unit 210 may further include a separation kit. Hereinafter, an example of separating blood tumor cells using a separation kit will be described.

FIG. 4 is a perspective view illustrating a separation kit used for separating target cells in a blood cell test vehicle according to an embodiment of the present invention. FIG. 5 is a cross- Is an exploded perspective view showing a separation kit used in the process.

4 and 5, the separation kit 220 used in the separation process of the target cells in the blood cell inspection vehicle according to the embodiment of the present invention includes a frame part 221, a filter part 225, And a hydrophobic film 227.

The frame part 221 can be a slide glass, for example, which is capable of placing a sample and moving it to the analysis equipment.

Here, the shape of the frame portion 221 is not limited to any particular shape. That is, the frame portion 221 may have various shapes such as a disk shape as well as an angular shape such as a rectangular shape.

Meanwhile, the frame part 221 may be made of various materials including glass, resin, and the like. Further, the frame portion 221 may include all of the sample and the sample (cell, etc.) on which the sample is placed. For example, the frame part 221 may include not only a slide and a plate but also a component having a holding, supporting and collecting function, and further, a chip on which a cell is placed. May vary.

An inflow hole 222 may be formed through the frame portion 221.

The position of the inflow hole 222 is not particularly limited, and the number of the inflow holes 222 is not limited to a specific number. In addition, the inflow hole 222 may be formed in an angular shape such as a rectangular shape as well as a circular shape as shown. The cell mixture solution 230 to be described later may be introduced into the inflow hole 222.

The filter unit 225 may be provided to cross the inflow hole 222.

Specifically, the filter unit 225 may be provided below the inflow hole 222. The lower surface of the filter unit 225 may have the same height as the lower surface of the frame unit 221 or protrude from the lower surface of the frame unit 221 or may be positioned lower than the lower surface of the frame unit 221.

The filter unit 225 is formed in a shape corresponding to the inflow hole 222 and is inserted into the lower inner side of the inflow hole 222 or formed larger than the inflow hole 222, Or may be provided in various ways.

A plurality of micro holes 226 may be formed in the filter unit 225. The microhole 226 may have the same size throughout the filter portion 225 and may be formed uniformly throughout the filter portion 225.

The microholes 226 of the filter unit 225 are formed by various methods including etching or electroforming using MEMS (Micro Electro-Mechanical Systems) .

The microhole 226 of the filter unit 225 separates the target cells from the cell mixture by filtering the target cells 231 (see FIG. 7) from passing through the cell mixture solution 230 flowing into the inlet holes 222 I can leave. To this end, the microhole 226 may have a smaller size than the target cell.

The target cell 231 can be filtered as the microhole 226 is formed smaller than the target cell 231. In this case, however, not all of the non-target cells pass through the microhole 226. That is, some non-target cells such as a liquid (not shown) and a red blood cell 232 (see FIG. 7C) except for the target cells in the cell mixture solution 230, Some non-target cells such as white blood cells 233 (see FIG. 7 (e)) may not pass through the microholes 226, but may pass through the target cells 231 And may remain on the filter unit 225.

It is preferable that the filter unit 225 is excellent in flatness and has no bending and pattern in the vertical direction on the surface. Focusing can be accurately performed at the time of imaging the cell mixture liquid or the target cell on the filter unit 225.

As one form of the filter unit 225, the filter unit 225 may have a shape having a mesh. However, the mesh-type filter portion is merely an example, and the filter portion 225 can be interpreted to include all types of filters having a filtering function through the microstructure.

The hydrophobic film 227 may be coated on the filter portion 225. The hydrophobic membrane 227 may be coated on the entire surface of the filter portion 225, including the inner surface of the microhole 226. Thus, the filter unit 225 may have hydrophobicity.

The hydrophobic membrane 227 is coated on the filter part 225 so that the cell mixture solution 230 flowing into the inflow hole 222 can be concentrated on the filter part 225. That is, the hydrophobic membrane 227 can help the cell mixture liquid 230 having the attractive force between the cells in the cell mixture liquid, the surface tension of the liquid in the cell mixture liquid, etc., to remain on the filter unit 225 in a dripped form.

In addition, the filter unit 225 may be coated with a hydrophilic film (not shown). When the hydrophilic membrane is coated on the filter unit 225, the hydrophilic membrane can prevent droplets of the mixed cell mixture from flowing into the membrane.

The hydrophilic film 227 or the hydrophilic film 227 is coated on the filter portion 225 or the hydrophobic film 227 is coated on the hydrophilic film 227 in a coated state or the hydrophilic film is converted and coated on the hydrophobic film ≪ / RTI > That is, the filter unit 225 may have a hydrophilic surface (Switchable Surface).

The coating of the hydrophobic film or the hydrophilic film with respect to the surface of the filter unit 225 can be appropriately selected depending on, for example, the kind of the specimen, the kind of the target cell,

The coating of the hydrophobic film 227 or the hydrophilic film may be accomplished by varying the voltage supplied to the filter portion 225 in the coating process. This coating process may proceed with the filter portion 225 provided in the frame portion 221 through which the hydrophobic film coating or the hydrophilic film coating on the filter portion 225 can be conveniently and quickly converted.

Further, the separation kit 220 may further include an absorption pad 229.

The absorbing pad 229 may be provided on the lower surface of the frame portion 221. The absorption pad 229 can be brought into close contact with the lower surface of the frame portion 221 to absorb liquid and non-target cells passing through the filter portion 225.

The absorbent pad 229 may be made of, for example, paper, but is not limited thereto. That is, the absorbent pad 229 may be made of a material suitable for absorbing liquid and non-target cells in the cell mixture solution 230, thereby improving the absorbency and storage properties of liquid and non-target cells separated from the cell mixture have.

The absorbent pad 229 may be provided separately from the frame 221 and may be in close contact with the lower surface of the frame 221 when it is desired to absorb the cell mixture of the filter 225. However, if necessary, the absorbent pad 229 may be provided in close contact with the lower surface of the frame portion 221.

FIG. 6 is a flow chart of a blood cell separation process using a separation kit for use in a target cell separation process in a blood cell test vehicle according to an embodiment of the present invention. FIG. FIG. 2 is a process example showing a blood cell separation process using a separation kit used in a process of separating target cells from a vehicle. FIG.

As shown in FIGS. 6 and 7 (a) to 7 (c), in the method of separating blood cells using a mobile blood cell testing apparatus according to an embodiment of the present invention, (S710) flowing into the inflow hole 222 formed in the hydrophobic membrane 227 so as to be high on the filter unit 225 formed with the hydrophobic membrane 227.

Here, the cell mixture solution 230 may include physiological fluids such as saliva, sweat, and urine of humans or animals, blood, serum, and the like.

In an embodiment of the present invention, the cell mixture 230 may be blood, and the target cell 231 may be CTCs (Circulating Tumor Cells) as described above. The non-target cell may be a cell other than a blood tumor cell, for example, a red blood cell 232 and a white blood cell 233.

The cell mixture solution 230 may be supplied to the inflow hole 222 using a mechanism such as a pipette or the like. At this time, when the cell mixture solution 230 is supplied to the inflow hole 222, the lower end of the pipette is positioned close to the inflow hole 222 to minimize the distance of free fall of the cell mixture solution 230, 230 can be made smaller.

Alternatively, the cell mixture solution 230 may be introduced into a liquid sample by centrifugal force, for example, a cell centrifuge 213 (see FIG. 3) And may be supplied to the ball 222.

At this time, since the surface of the filter unit 225 is coated with the hydrophobic film 227, the cell mixture liquid 230 flowing into the inlet hole 222 may be accumulated in the form of droplets on the filter unit 225 .

The hydrophobic film 227 may be coated as the filter unit 225 is provided in the frame unit 221 and a voltage is supplied to the filter unit 225.

The filter unit 225 may have a microhole 226. For example, the filter unit 225 may have a mesh shape. The hydrophobic film 227 may be coated on the entire surface of the filter portion 225.

7 (d), the absorbent pad 229 is brought into close contact with the lower surface of the frame portion 221 so that liquid and rain in the cell mixture liquid 230, which is held on the filter portion 225, The target cell may be passed through the filter unit 225 to be absorbed by the absorption pad 229 and the target cell 231 in the cell mixture liquid may be separated by the filter unit 225 to proceed to step S720.

That is, as the absorbent pad 229 is brought into close contact with the lower surface of the frame portion 221, the liquid in the cell mixture solution 230 on the filter portion 225 can be absorbed into the absorbent pad 229. At this time, non-target cells smaller in size than the microholes 226 of the filter unit 225, for example, the red blood cells 232 and the like can be absorbed by the absorption pad 229.

The target cell 231 is filtered on the filter unit 225 by absorbing the liquid and some non-target cells into the absorption pad 229 in the cell mixture liquid 230 on the filter unit 225 It remains. Such a separation method of the target cell 231 can effectively prevent damage to the target cell since it hardly gives a physical impact to the target cell as in the conventional cell separation apparatus.

The absorbent pad 229 may be made of a material suitable for absorbing liquid and non-target cells except the target cells in the cell mixture solution 230, thereby improving the absorbency and storage properties of liquid and non-target cells separated from the cell mixture . The absorbent pad 229 may be made of, for example, paper, but is not limited thereto.

On the other hand, after the step (S710) of making the cell mixture liquid high on the filter part 225, the step of converting and coating the hydrophobic film 227 of the filter part 225 into the hydrophilic film may be further performed. Here, the hydrophilic film may be transformed as voltage is applied to the filter unit 225 in a state where the filter unit 225 is provided in the frame unit 221.

Further, by supplying the voltage applied to the filter unit 225 according to predetermined conditions, not only the hydrophobic film can be converted into the hydrophilic film, but also the hydrophilic film can be converted and coated with the hydrophobic film.

When the hydrophilic membrane is transformed and coated on the filter unit 225, the superimposition phenomenon of the cell mixture on the filter unit 225 is removed, so that absorption at the absorption pad 229 can be more smoothly performed.

Meanwhile, the process in which the hydrophilic film is coated on the surface of the filter unit 225 after the cell mixture solution is stored on the filter unit 225 may be performed before the absorption pad 229 is adhered to the lower surface of the frame unit 221 . In this case, the above-described process in which the absorbent pad 229 is brought into close contact with the lower surface of the frame portion 221 may be omitted if necessary. That is, when the surface of the filter unit 225 is converted and coated with a hydrophilic film to allow the liquid and non-target cells in the cell mixture liquid to be sufficiently discharged, a step of attaching the absorbent pad 229 to the lower surface of the frame unit 221 is omitted . However, when it is impossible to have sufficient effect of discharging liquid and non-target cells in the cell mixture liquid by simply coating the hydrophilic film on the surface of the filter part 225, The process of attaching the absorbent pad 229 to the bottom surface may proceed.

7 (e), the step (S730) in which the absorbing pad 229 is removed from the frame part 221 may proceed.

The absorbent pad 229 can be removed from the frame portion 221 after sufficient absorption of the liquid and some non-target cells from the cell mixture is made.

After the liquid and non-target cells in the cell mixture solution 230 are absorbed by the absorption pad 229, only some non-target cells such as the target cells 231 and the white blood cells 233 may remain on the filter portion 225 .

The micro holes 226 of the filter unit 225 may be formed to have a smaller size than the target cells 231 so that the target cells are filtered. The microholes 226 of the filter unit 225 may be appropriately formed according to the size of the target cells. This allows the filter unit 225 to effectively separate the target cells 231 from the cell mixture solution 230 and to discharge the separated liquid and non-target cells from the cell mixture 230 Can be adjusted.

The frame portion 221 from which the absorption pad 229 has been removed can be moved to a measuring device such as a microscope.

As shown in FIGS. 7 (f) and 7 (g), the step of dyeing only the target cells 231 left in the filter unit 225 by the introduction of the dyeing liquid 240 into the inflow holes 222 (S740) may be performed.

That is, the blood cell separation process according to an embodiment of the present invention may include separating the target cells and staining the separated target cells.

Here, the staining liquid 240 may be such that only the target cells 231 are stained with a specific color (for example, red). Therefore, the dyeing solution 240 introduced into the inflow hole 222 can stain the target cells 231 remaining on the filter unit 225 with a specific color.

The inspector can count the number of target cells stained using a measuring device such as a microscope or easily find the number of target cells through the cell counter 214.

1 to 3, the storage unit 250 can store the target cells separated from blood in a living cell in a viable state. To this end, the storage unit 250 may include a refrigerator 251. The refrigerator 251 may be a small refrigerator in consideration of the working space 111 of the accommodating portion 110.

The centrifugal separator 211, the rotator 212, the cell centrifuge 213, the cell counter 214, the carbon dioxide incubator 215, the clean bench 216 and the refrigerator 251 are connected to the flow of the inspection process, And the like, and a cradle or the like may be further provided if necessary.

On the other hand, the fuel cell 300 can supply a driving voltage to the inspection apparatus 200.

The fuel cell 300 may include a fuel cell stack (not shown) that generates electrical energy from the electrochemical reaction of the reaction gas.

The fuel cell stack may have an anode functioning as a cathode and a cathode functioning as an anode. Hydrogen may be supplied as fuel to the anode, and oxygen may be supplied to the cathode as an oxidant. Also, a membrane may be provided between the anode and the cathode, and hydrogen cations (H +) generated in the anode may move to the cathode electrode through the membrane. In the fuel cell stack, hydrogen as a fuel and oxygen as an oxidant are supplied to generate a reaction gland, electrical energy, heat, and water (water).

The fuel cell 300 also includes a hydrogen supply system for supplying hydrogen as fuel to the fuel cell stack, an air supply system for supplying air to the fuel cell stack including oxygen as an oxidant necessary for the electrochemical reaction, And a heat and moisture management system for controlling the operation temperature of the fuel cell stack and performing a moisture management function by releasing heat as a by-product of chemical reaction to the outside. That is, in the present invention, the fuel cell 300 can be interpreted as a fuel cell system including the above-described configurations.

The fuel cell 300 may be provided separately from a vehicle battery (not shown) used for starting the inspection vehicle 100 or the like. The mounting position of the fuel cell 300 and the like are not particularly limited.

In the present invention, the test apparatus 200 can be operated using the fuel cell 300 without starting the blood cell testing vehicle 100. Since the operating voltage of the inspecting apparatus 200 can be supplied using the fuel cell 300 as described above, vibrations that may occur when the inspecting vehicle 100 is started may not be generated, 200) can be stably performed.

The blood cell testing vehicle 100 may further include an environment maintaining device 400 and the environment maintaining device 400 may receive a driving voltage from the fuel cell 300. [

The environment maintaining apparatus 400 can adjust and maintain the internal environmental conditions of the work space 111 of the accommodating portion 110. [ The internal environmental conditions may include temperature, humidity, and the like. The environment maintaining apparatus 400 may have a constant temperature function for keeping the temperature inside the work space 111 constant.

The blood cell testing vehicle 100 may further include an air conditioner 500, and the air conditioner 500 may receive a driving voltage from the fuel cell 300.

 The air conditioner 500 can adjust and maintain the air condition of the work space 111 of the accommodating portion 110. The air conditioner 500 can circulate or exhaust the air in the work space 111 and thereby maintain a good air cleanliness in the work space 111. [

In addition, the air conditioner 500 can circulate or exhaust gases and gases. Accordingly, the air conditioning apparatus 500 can have a function of discharging pollutants in the inspection vehicle 100, such as infectious samples, wastes, etc., as well as the functions of circulation and exhaust of the air including the air as described above.

Since the blood cell testing vehicle 100 according to the present invention can receive the driving voltages of the inspection apparatus 200, the environment maintaining apparatus 400 and the air conditioning apparatus 500 from the fuel cell 300, ) Can be inspected without turning on the ignition. Therefore, the vibration of the vehicle can be minimized, thereby improving the inspection quality.

On the other hand, a server may be installed in the near or remote area of the blood cell testing vehicle 100 according to the present invention. Since the process information according to the inspection of the blood cells can be transmitted to the server and managed, the inspection process can be efficiently managed.

It is also possible to add a means for automatically monitoring the progress of each step of the inspection process. For example, a barcode or an RFID tag is attached to a specimen or a slide containing a sample, and a reader for recognizing these tags in a contact or non-contact manner is provided, whereby management of sample and sample information and inspection process management can be efficiently performed .

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

100: Blood cell test vehicle
110:
200: Inspection device
220: separation kit
300: Fuel cell
400: environment maintaining device
500: air conditioner

Claims (4)

A receiving portion in which a work space is formed;
An inspection device installed in the work space for separating the target cells from the specimen and storing the separated target cells in a live cell state; And
And a fuel cell for supplying a driving voltage to the inspection apparatus.
The method according to claim 1,
Wherein the accommodating portion further includes an environment maintaining device for receiving a driving voltage from the fuel cell and maintaining an internal environmental condition of the work space.
3. The method of claim 2,
Wherein the internal environmental condition includes temperature and humidity.
The method according to claim 1,
Further comprising an air conditioning device for receiving driving voltage from the fuel cell, circulating air in the working space, and discharging contaminants from the fuel cell.

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