CN111007221A - Automatic classification counting system for algae in water body - Google Patents
Automatic classification counting system for algae in water body Download PDFInfo
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- CN111007221A CN111007221A CN201911340768.2A CN201911340768A CN111007221A CN 111007221 A CN111007221 A CN 111007221A CN 201911340768 A CN201911340768 A CN 201911340768A CN 111007221 A CN111007221 A CN 111007221A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
Abstract
The application provides a water alga automatic classification counting system, relates to the water quality testing field. The detection device controls the sampling device to sample the water sample to be detected stored in the sample storage device according to the preset sampling amount, and controlling the sampling device to inject the taken water sample into the target sample carrying assembly, driving the target sample carrying assembly to move between the lighting device and the observation device by the transmission device, so that the observation device carries out microscopic image acquisition on the water sample carried by the target sample carrying assembly under the irradiation of the lighting device to obtain a microscopic image corresponding to the water sample, then the detection device carries out image analysis on the microscopic image collected by the observation device according to the preset sampling amount to obtain the algae classification counting result corresponding to the water sample, therefore, the automatic operation of the algae detection operation is realized, the qualitative detection and the quantitative detection of the algae are completed, the manual participation degree in the algae detection process is reduced, and the algae detection precision is improved.
Description
Technical Field
The application relates to the field of water quality detection, in particular to an automatic classification counting system for algae in a water body.
Background
With the continuous advance of industrial development and urban construction, the problem of environmental pollution also becomes a key problem of wide social attention at present, and the problem of water quality pollution is an extremely important problem in the problem of environmental pollution, wherein the monitoring of algae in water is an important content in the current stage of water quality monitoring. However, in the prior art, the qualitative detection of algae in a water sample is mainly focused, the influence of the quantitative detection on the algae detection result is ignored, and the detection personnel needs to manually control each physical device required by the algae detection to perform the algae detection test, so that the problems of low detection precision and high human resource consumption exist.
Disclosure of Invention
In view of this, an object of the present application is to provide an automatic classification and counting system for algae in water, which can automatically perform detection operations such as quantitative sampling, sample transmission, algae observation and result output on a water sample to be detected, and simultaneously realize qualitative detection and quantitative detection on algae, reduce human involvement in an algae detection process, and improve algae detection accuracy.
In order to achieve the above purpose, the embodiments of the present application employ the following technical solutions:
in a first aspect, an embodiment of the present application provides an automatic classification and counting system for algae in a water body, the system includes a sample storage device, a sampling device, an illumination device, an observation device, a transmission device, a detection device, and a plurality of sample loading assemblies, wherein the plurality of sample loading assemblies are detachably embedded on the transmission device;
the detection device is electrically connected with the sampling device and is used for controlling the sampling device to sample the water sample to be detected stored in the sample storage device according to a preset sampling amount and controlling the sampling device to inject the taken water sample into the target sample loading assembly;
the transmission device is used for driving the target sample loading assembly to move between the illuminating device and the observing device;
the observation device is used for carrying out microscopic image acquisition on the water sample borne by the target sample loading assembly under the irradiation of the illumination device to obtain a microscopic image corresponding to the water sample;
the observation device is electrically connected with the detection device and used for sending the acquired microscopic image to the detection device, so that the detection device performs image analysis on the microscopic image according to the preset sampling amount to obtain an algae classification counting result corresponding to the water sample.
In an optional embodiment, the detection device is further electrically connected to the transmission device, and is configured to control the transmission device to drive the target sample loading assembly to move between the illumination device and the observation device when the sampling device completes a water sample injection operation.
In an optional embodiment, the detection device is further electrically connected to the illumination device, and is configured to control the illumination device to illuminate the target sample loading assembly when the target sample loading assembly is located between the illumination device and the observation device;
the detection device is also used for controlling the observation device to carry out microscopic image acquisition on the water sample carried by the target sample carrying assembly when the target sample carrying assembly is positioned between the illumination device and the observation device.
In an alternative embodiment, the sampling device comprises a mechanical arm, a sampling needle and a control unit;
the sampling needle is mounted on the mechanical arm, and the control unit is electrically connected with the mechanical arm and is used for controlling the mechanical arm to drive the sampling needle to move;
the control unit is also electrically connected with the sampling needle and is used for controlling the sampling needle to carry out water sample extraction operation or water sample injection operation.
In an alternative embodiment, the robotic arm comprises a support base, a rotating rod, a first telescoping rod, and a second telescoping rod;
one end of the rotating rod is fixedly connected with one end of the first telescopic rod, the other end of the first telescopic rod is fixedly connected with one end of the second telescopic rod, the other end of the second telescopic rod is fixedly connected with the sampling needle, and the other end of the rotating rod is movably mounted on the supporting base;
the rotating rod is electrically connected with the control unit and is used for driving the first telescopic rod, the second telescopic rod and the sampling needle to rotate relative to the supporting base under the control of the control unit;
the second telescopic rod and the first telescopic rod are respectively electrically connected with the control unit and used for performing telescopic operation under the control of the control unit.
In an alternative embodiment, the sample assembly comprises a transparent slide and a transparent cover slip;
a groove with the extending direction parallel to the length extending direction of the transparent glass slide and two mounting bosses arranged on two sides of the groove are formed on the surface of one side of the transparent glass slide;
the transparent cover glass covers the two mounting bosses and is fixedly connected with the two mounting bosses, wherein the length of the transparent cover glass in the length extending direction of the transparent glass slide is smaller than that of the groove in the length extending direction of the transparent glass slide, so that a sample inlet is formed in the transparent glass slide.
In an optional embodiment, the transmission device comprises a fixed frame, a driving unit, a driving wheel, a driven wheel and a conveying belt;
the driving wheel and the driven wheel are respectively arranged on two opposite sides of the fixed frame and can rotate relative to the fixed frame;
the conveying belt is sleeved on the driving wheel and the driven wheel and is meshed with the driving wheel and the driven wheel, and a plurality of accommodating holes for accommodating the sample loading assemblies are formed in the conveying belt at intervals;
the driving unit is electrically connected with the driving wheel and used for driving the driving wheel to rotate and driving the driven wheel and the conveying belt to move.
In an alternative embodiment, the lighting device is arranged on the fixed frame at a central position between the driving wheel and the driven wheel and is fixedly connected with the fixed frame, wherein the lighting direction of the lighting device is opposite to the surface of the conveyor belt tangent to the driving wheel and the driven wheel.
In an alternative embodiment, the observation device comprises a microscope and a camera;
the objective lens of the microscope is positioned on the light path of the lighting device and is arranged opposite to the lighting device at intervals of the conveyor belt;
the camera is arranged on an ocular of the microscope and is used for collecting microscopic images of the water sample observed by the microscope.
In an alternative embodiment, the system further comprises a power supply;
the power supply device is electrically connected with the sampling device, the illuminating device, the observing device, the transmission device and the detection device and is used for providing electric energy for the sampling device, the illuminating device, the observing device, the transmission device and the detection device.
Compared with the background art, the method has the following beneficial effects:
the application controls the sampling device to sample a water sample to be detected stored in the sample storage device according to a preset sampling amount through the detection device electrically connected with the sampling device, controls the sampling device to inject the taken water sample into the target sample carrying assembly, drives the target sample carrying assembly to move between the illuminating device and the observation device through the transmission device, enables the observation device to carry out microscopic image acquisition on the water sample carried by the target sample carrying assembly under the illumination of the illuminating device to obtain a microscopic image corresponding to the water sample, then sends the acquired microscopic image to the detection device electrically connected with the observation device through the observation device, enables the detection device to carry out water sample image analysis on the microscopic image according to the preset sampling amount to obtain an algae classification counting result corresponding to the water sample, thereby realizing the automatic operation of algae detection operation, the algae detection method has the advantages that qualitative detection and quantitative detection of algae are completed, manual participation in an algae detection process is reduced, and algae detection precision is improved.
In order to make the aforementioned objects, features and advantages of the present application more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.
FIG. 1 is a schematic diagram illustrating a system of an automatic classification and counting system for algae in a water body according to an embodiment of the present disclosure;
FIG. 2 is a second schematic diagram illustrating the system components of the system for automatically classifying and counting algae in a water body according to the embodiment of the present application;
fig. 3 is a schematic structural diagram of a sampling device according to an embodiment of the present application;
FIG. 4 is a schematic structural diagram of a sample loading assembly according to an embodiment of the present disclosure;
FIG. 5 is a schematic structural diagram of a transmission provided in an embodiment of the present application;
FIG. 6 is a third schematic view illustrating a system configuration of an automatic classification and counting system for algae in a water body according to an embodiment of the present invention;
FIG. 7 is a fourth schematic view illustrating a system of an automatic classification and counting system for algae in a water body according to an embodiment of the present disclosure;
fig. 8 is a fifth schematic view illustrating a system configuration of an automatic classification and counting system for algae in a water body according to an embodiment of the present application.
Icon: 10-an automatic classification counting system for algae in the water body; 11-a sample storage device; 12-a sampling device; 13-a lighting device; 14-a viewing device; 15-a transmission; 16-a detection device; 17-a sample loading module; 121-a robotic arm; 122-a support base; 123-rotating rod; 124-a first telescopic rod; 125-a second telescoping rod; 126-a sampling needle; 127-a control unit; 171-transparent glass slide; 172-transparent cover glass; 173-a groove; 174-mounting bosses; 151-a mount; 152-a drive unit; 153-a drive wheel; 154-a driven wheel; 155-a conveyor belt; 156-containment hole; 18-power supply means.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. The components of the embodiments of the present application, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present application without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that in the description of the present application, it is understood that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship which is usually placed when the product of the application is used, or the orientation or positional relationship which is usually understood by those skilled in the art, and are only for the convenience of describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present application. Moreover, relational terms such as "first" and "second," and the like, may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions.
In the description of the present application, it is further noted that, unless expressly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
Some embodiments of the present application will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.
Referring to fig. 1 and fig. 2 in combination, fig. 1 is a schematic diagram of a system composition of an automatic classification and counting system 10 for algae in a water body according to an embodiment of the present application, and fig. 2 is a second schematic diagram of the system composition of the automatic classification and counting system 10 for algae in a water body according to the embodiment of the present application. In the embodiment of the present application, the system 10 for automatically classifying and counting algae in water can realize automatic operation of algae detection operation, complete qualitative detection and quantitative detection of algae, reduce human involvement in algae detection process, and improve algae detection precision. The automatic classification and counting system 10 for algae in water comprises a sample storage device 11, a sampling device 12, an illuminating device 13, an observing device 14, a transmission device 15, a detecting device 16 and a plurality of sample loading assemblies 17.
In this embodiment, the sample storage device 11 is used for storing samples of water to be tested. The sample storage device 11 comprises at least one sample storage tank, and at least one to-be-detected water sample can simultaneously exist in the sample storage device 11 through the at least one sample storage tank, so that the automatic water body algae sorting and counting system 10 can conveniently extract a certain amount of to-be-detected water sample from the sample storage device 11 to perform algae detection. In the process, the sample accommodating tank can perform standing concentration on the water sample to be detected for 24 hours, so that the automatic water body algae sorting and counting system 10 can realize quantitative detection of algae.
In this embodiment, the sampling device 12 is used for performing a water sample extraction operation or a water sample injection operation on a water sample to be detected. Specifically, the sampling device 12 is electrically connected to the detection device 16, and is configured to sample the water sample to be detected stored in the sample storage device 11 according to a preset sampling amount under the control of the detection device 16, and then inject the extracted water sample into a certain carrying device for carrying the sample to perform algae detection under the control of the detection device 16.
The detection device 16 can control the sampling device 12 to perform the water sample extraction operation and the water sample injection operation at a first preset time interval, so that the automatic classification and counting system 10 for algae in water body can perform algae detection on the water sample to be detected loaded on the plurality of load-bearing devices.
Optionally, referring to fig. 3, fig. 3 is a schematic structural diagram of the sampling device 12 according to an embodiment of the present disclosure. In the embodiment of the present application, the sampling device 12 includes a mechanical arm 121, a sampling needle 126 and a control unit 127, wherein the mechanical arm 121, the sampling needle 126 and the control unit 127 cooperate to extract the water sample from the sample storage device 11 and inject the extracted water sample into the carrier.
The sampling needle 126 is mounted on the mechanical arm 121, and the control unit 127 is electrically connected to the mechanical arm 121 and is configured to control the mechanical arm 121 to drive the sampling needle 126 to move. For example, the control unit 127 may control the mechanical arm 121 to drive the sampling needle 126 to extend into the sample storage device 11, so that the sampling needle 126 is inserted below the liquid level of the water sample to be detected, may control the mechanical arm 121 to drive the sampling needle 126 to be away from the sample storage device 11, and may control the mechanical arm 121 to drive the sampling needle 126 to be close to a carrying device to be injected with the water sample, so that the sampling needle 126 can inject the extracted water sample into the carrying device.
The control unit 127 is further electrically connected to the sampling needle 126, and is configured to control the sampling needle 126 to perform a water sample extraction operation or a water sample injection operation. When the sampling needle 126 extends below the liquid level of the water sample to be detected, the control unit 127 can control the sampling needle 126 to perform water sample extraction operation according to a preset sampling amount, and when the sampling needle 126 extends into the bearing device, can control the sampling needle 126 to inject the extracted water sample into the bearing device.
The control unit 127 is electrically connected to the detection device 16, and is configured to receive a control instruction sent by the detection device 16 to the sampling device 12, and control the mechanical arm 121 and the sampling needle 126 according to the control instruction.
In this embodiment, the robot arm 121 may include a support base 122, a rotating rod 123, a first telescopic rod 124 and a second telescopic rod 125. One end of the rotating rod 123 is fixedly connected to one end of the first telescopic rod 124, the other end of the first telescopic rod 124 is fixedly connected to one end of the second telescopic rod 125, the other end of the second telescopic rod 125 is fixedly connected to the sampling needle 126, and the other end of the rotating rod 123 is movably mounted on the supporting base 122. The rotating rod 123 can rotate relative to the supporting base 122, the first telescopic rod 124 and the second telescopic rod 125 can both perform an extending operation or a shortening operation, and the rotating rod 123, the first telescopic rod 124 and the second telescopic rod 125 cooperate with each other to form the mechanical arm 121 having a U-shaped structure, and drive the sampling needle 126 to move to the sample storage device 11 or the carrying device.
The rotating rod 123 is electrically connected to the control unit 127, and is configured to drive the first telescopic rod 124, the second telescopic rod 125 and the sampling needle 126 to rotate relative to the supporting base 122 under the control of the control unit 127. The second telescopic rod 125 and the first telescopic rod 124 are electrically connected to the control unit 127, respectively, and are configured to perform a telescopic operation under the control of the control unit 127.
In the present embodiment, the sample loading assembly 17 is used as a carrying device in the automatic classification and counting system 10 for algae in the water body. The sample loading assemblies 17 are arranged in the transmission device 15 in a mutually spaced and detachable embedded manner, and the transmission device 15 can drive the sample loading assemblies 17 to move between the illuminating device 13 and the observation device 14. After the sampling device 12 injects the sample to be detected into a certain sample loading assembly 17, namely the target sample loading assembly 17, mounted on the transmission device 15, the transmission device 15 can drive all the sample loading assemblies 17 to move until the target sample loading assembly 17 is moved between the illumination device 13 and the observation device 14, and at this time, the sample loading assembly 17 adjacent to the target sample loading assembly 17 in the direction opposite to the moving direction of the transmission device 15 can also move to the vicinity of the sampling device 12, so that the sampling device 12 can inject the sample to be detected into the sample loading assembly 17 in the vicinity thereof, and the sample loading assembly 17 is used as a new target sample loading assembly 17.
The transmission device 15 can also drive all the sample carrying assemblies 17 to move according to a second preset time interval, so that the target sample carrying assembly 17 carrying the water sample to be detected can be moved to a position between the lighting device 13 and the observation device 14, and the observation device 14 and the lighting device 13 are matched with each other to perform algae observation operation on the water sample carried by the target sample carrying assembly 17. In this process, the interval value of the second preset time interval is not less than the interval value of the first preset time interval.
Optionally, please refer to fig. 4, fig. 4 is a schematic structural diagram of the sample loading assembly 17 provided in the embodiment of the present application. In the present embodiment, the sample assembly 17 includes a transparent slide 171 and a transparent cover glass 172. A groove 173 having an extending direction parallel to the longitudinal extending direction of the transparent slide 171 and two mounting bosses 174 disposed at both sides of the groove 173 are formed at a central position on one side surface of the transparent slide 171. The transparent cover glass 172 covers the two mounting bosses 174 and is fixedly connected with the two mounting bosses 174.
The length of the transparent cover glass 172 in the length extending direction of the transparent slide 171 is smaller than the length of the groove 173 in the length extending direction of the transparent slide 171, so as to form a sample inlet on the transparent slide 171, so that the sampling device 12 can inject a sample to be detected into the sample assembly 17 through the sample inlet, ensure that the sample fills the gap between the transparent slide 171 and the transparent cover glass 172, and exhaust the air between the transparent slide 171 and the transparent cover glass 172.
Optionally, referring to fig. 5, fig. 5 is a schematic structural diagram of the transmission device 15 according to the embodiment of the present application. In the embodiment of the present application, the transmission device 15 may include a fixing frame 151, a driving unit 152, a driving wheel 153, a driven wheel 154, and a transmission belt 155.
In this embodiment, the driving wheel 153 and the driven wheel 154 are respectively disposed at two opposite sides of the fixed frame 151, and are movably connected to the fixed frame 151. The driving wheel 153 and the driven wheel 154 are both rotatable relative to the fixed frame 151.
The transmission belt 155 is sleeved on the driving wheel 153 and the driven wheel 154, and is engaged with the driving wheel 153 and the driven wheel 154, so that the driving wheel 153 can drive the driven wheel 154 to rotate through the transmission belt 155 when rotating, and accordingly, the transmission belt 155 rotates around the driving wheel 153 and the driven wheel 154. The conveyor belt 155 is provided with a plurality of accommodating holes 156 for fixedly clamping and accommodating the sample loading assembly 17 at intervals, and the accommodating holes 156 are through-hole structures. When the sample loading assembly 17 is inserted into the corresponding accommodating hole 156 and the driving wheel 153 starts to rotate, the conveyor belt 155 starts to move under the driving of the driving wheel 153, so as to drive the sample loading assembly 17 to move.
The driving unit 152 is electrically connected to the driving wheel 153, and is configured to drive the driving wheel 153 to rotate, so as to drive the driven wheel 154 and the conveyor belt 155 to move.
Referring to fig. 1 again, in the present embodiment, the illumination device 13 is disposed on the fixing frame 151 at a central position between the driving wheel 153 and the driven wheel 154, and is fixedly connected to the fixing frame 151, wherein an illumination direction of the illumination device 13 is opposite to a surface of the conveyor belt 155 tangent to the driving wheel 153 and the driven wheel 154, so that when the conveyor belt 155 carries the target sample loading assembly 17 to move to a position right above the illumination device 13, the illumination device 13 can be directly illuminated on the sample loaded on the target sample loading assembly 17.
In one embodiment of this embodiment, the illumination device 13, whether facing the target loading assembly 17 or not, operates normally and is always illuminated. In another embodiment of this embodiment, the operation time of the illumination device 13 and the operation time of the transmission device 15 are staggered, when the transmission device 15 stops operating, the illumination device 13 starts operating, and when the transmission device 15 starts operating, the illumination device 13 stops operating, wherein when the transmission device 15 operates, the target loading assembly 17 moves between the illumination device 13 and the observation device 14.
In this embodiment, the observation device 14 is configured to perform microscopic image acquisition on the water sample carried by the target sample carrying assembly 17 under the illumination of the illumination device 13, so as to obtain a microscopic image corresponding to the water sample. The observation device 14 may include a microscope and a camera, and an objective lens of the microscope is located on the optical path of the illumination device 13 and is disposed to face the illumination device 13 with the conveyor belt 155 interposed therebetween. When the light emitted from the lighting device 13 irradiates the target sample loading assembly 17, the microscope can adjust the focal length accordingly, and observe the irradiated water sample. The camera is arranged on an ocular of the microscope and is used for collecting microscopic images of the water sample observed by the microscope. The camera can select 100-1000 different visual fields for photographing during image acquisition, and microscopic image acquisition operation is achieved.
In this embodiment, the observation device 14 is electrically connected to the detection device 16, and is configured to send the acquired microscopic image to the detection device 16, so that the detection device 16 performs image analysis on the microscopic image according to the preset sampling amount to obtain an algae classification counting result corresponding to the water sample. The detection device 16 can count the number of each algae substance in each visual field in the water sample, and then perform algae density calculation according to the preset sampling amount and the algae classification counting result corresponding to the water sample to obtain the respective corresponding algae densities of different algae substances in the water sample, so as to automatically perform detection operations such as quantitative sampling, sample transmission, algae observation and result output on the water sample to be detected, simultaneously realize qualitative detection and quantitative detection on algae, reduce the artificial participation degree in the algae detection process, and improve the algae detection precision.
Optionally, referring to fig. 6, fig. 6 is a third schematic diagram illustrating a system composition of the automatic classification and counting system 10 for algae in a water body according to the embodiment of the present application. In the embodiment of the present application, the detecting device 16 is further configured to control the observing device 14 to perform microscopic image acquisition on the water sample carried by the target sample carrying assembly 17 when the target sample carrying assembly 17 is located between the illuminating device 13 and the observing device 14.
In this embodiment, the detecting device 16 is further electrically connected to the transmission device 15, and is configured to control the transmission device 15 to drive the target sample loading assembly 17 to move between the illuminating device 13 and the observing device 14 when the sampling device 12 completes a water sample injection operation on the target sample loading assembly 17.
Optionally, referring to fig. 7, fig. 7 is a fourth schematic diagram illustrating a system composition of the automatic classification and counting system 10 for algae in a water body according to an embodiment of the present application. In the embodiment of the present application, the detecting device 16 is further electrically connected to the illuminating device 13, and is configured to control the illuminating device 13 to illuminate the target sample loading assembly 17 when the target sample loading assembly 17 is located between the illuminating device 13 and the observing device 14.
Optionally, referring to fig. 8, fig. 8 is a fifth schematic diagram illustrating a system composition of the automatic classification and counting system 10 for algae in a water body according to an embodiment of the present application. In the embodiment of the present application, the system 10 for automatically classifying and counting algae in a water body may further include a power supply device 18.
In this embodiment, the power supply device 18 is electrically connected to the sampling device 12, the illuminating device 13, the observing device 14, the transmission device 15 and the detecting device 16, and is used for providing electric energy to the sampling device 12, the illuminating device 13, the observing device 14, the transmission device 15 and the detecting device 16, so as to ensure that the sampling device 12, the illuminating device 13, the observing device 14, the transmission device 15 and the detecting device 16 can operate normally.
In summary, in the automatic classification and counting system for algae in water provided by the present application, the detection device electrically connected to the sampling device controls the sampling device to sample the water sample to be detected stored in the sample storage device according to a preset sampling amount, controls the sampling device to inject the taken water sample into the target sample carrying assembly, drives the target sample carrying assembly to move to a position between the illumination device and the observation device by the transmission device, so that the observation device can perform microscopic image acquisition on the water sample carried by the target sample carrying assembly under the illumination of the illumination device to obtain a microscopic image corresponding to the water sample, and then sends the acquired microscopic image to the detection device electrically connected to the observation device by the observation device to perform image analysis on the microscopic image according to the preset sampling amount to obtain a classification and counting result of algae corresponding to the water sample, therefore, the automatic operation of the algae detection operation is realized, the qualitative detection and the quantitative detection of the algae are completed, the manual participation degree in the algae detection process is reduced, and the algae detection precision is improved.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (10)
1. An automatic classification counting system for algae in a water body is characterized by comprising a sample storage device, a sampling device, an illuminating device, an observing device, a transmission device, a detecting device and a plurality of sample carrying assemblies, wherein the plurality of sample carrying assemblies are detachably embedded on the transmission device;
the detection device is electrically connected with the sampling device and is used for controlling the sampling device to sample the water sample to be detected stored in the sample storage device according to a preset sampling amount and controlling the sampling device to inject the taken water sample into the target sample loading assembly;
the transmission device is used for driving the target sample loading assembly to move between the illuminating device and the observing device;
the observation device is used for carrying out microscopic image acquisition on the water sample borne by the target sample loading assembly under the irradiation of the illumination device to obtain a microscopic image corresponding to the water sample;
the observation device is electrically connected with the detection device and used for sending the acquired microscopic image to the detection device, so that the detection device performs image analysis on the microscopic image according to the preset sampling amount to obtain an algae classification counting result corresponding to the water sample.
2. The system of claim 1,
the detection device is electrically connected with the transmission device and used for controlling the transmission device to drive the target sample loading assembly to move to a position between the illumination device and the observation device when the sampling device finishes water sample injection operation.
3. The system of claim 1,
the detection device is also electrically connected with the illumination device and is used for controlling the illumination device to illuminate the target sample loading assembly when the target sample loading assembly is positioned between the illumination device and the observation device;
the detection device is also used for controlling the observation device to carry out microscopic image acquisition on the water sample carried by the target sample carrying assembly when the target sample carrying assembly is positioned between the illumination device and the observation device.
4. The system of claim 1, wherein the sampling device comprises a robotic arm, a sampling needle, and a control unit;
the sampling needle is mounted on the mechanical arm, and the control unit is electrically connected with the mechanical arm and is used for controlling the mechanical arm to drive the sampling needle to move;
the control unit is also electrically connected with the sampling needle and is used for controlling the sampling needle to carry out water sample extraction operation or water sample injection operation.
5. The system of claim 4, wherein the robotic arm comprises a support base, a rotating rod, a first telescoping rod, and a second telescoping rod;
one end of the rotating rod is fixedly connected with one end of the first telescopic rod, the other end of the first telescopic rod is fixedly connected with one end of the second telescopic rod, the other end of the second telescopic rod is fixedly connected with the sampling needle, and the other end of the rotating rod is movably mounted on the supporting base;
the rotating rod is electrically connected with the control unit and is used for driving the first telescopic rod, the second telescopic rod and the sampling needle to rotate relative to the supporting base under the control of the control unit;
the second telescopic rod and the first telescopic rod are respectively electrically connected with the control unit and used for performing telescopic operation under the control of the control unit.
6. The system of claim 1, wherein the sample assembly comprises a transparent slide and a transparent cover slip;
a groove with the extending direction parallel to the length extending direction of the transparent glass slide and two mounting bosses arranged on two sides of the groove are formed on the surface of one side of the transparent glass slide;
the transparent cover glass covers the two mounting bosses and is fixedly connected with the two mounting bosses, wherein the length of the transparent cover glass in the length extending direction of the transparent glass slide is smaller than that of the groove in the length extending direction of the transparent glass slide, so that a sample inlet is formed in the transparent glass slide.
7. The system of claim 1, wherein the transmission comprises a fixed frame, a driving unit, a driving wheel, a driven wheel and a conveying belt;
the driving wheel and the driven wheel are respectively arranged on two opposite sides of the fixed frame and can rotate relative to the fixed frame;
the conveying belt is sleeved on the driving wheel and the driven wheel and is meshed with the driving wheel and the driven wheel, and a plurality of accommodating holes for accommodating the sample loading assemblies are formed in the conveying belt at intervals;
the driving unit is electrically connected with the driving wheel and used for driving the driving wheel to rotate and driving the driven wheel and the conveying belt to move.
8. The system of claim 7,
the lighting device is arranged on the fixed frame and is positioned at the central position between the driving wheel and the driven wheel, and is fixedly connected with the fixed frame, wherein the lighting direction of the lighting device is right opposite to the surface of the conveying belt, which is tangent to the driving wheel and the driven wheel.
9. The system of claim 8, wherein the viewing device comprises a microscope and a camera;
the objective lens of the microscope is positioned on the light path of the lighting device and is arranged opposite to the lighting device at intervals of the conveyor belt;
the camera is arranged on an ocular of the microscope and is used for collecting microscopic images of the water sample observed by the microscope.
10. The system according to any one of claims 1-9, further comprising a power supply;
the power supply device is electrically connected with the sampling device, the illuminating device, the observing device, the transmission device and the detection device and is used for providing electric energy for the sampling device, the illuminating device, the observing device, the transmission device and the detection device.
Priority Applications (1)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112683572A (en) * | 2021-01-11 | 2021-04-20 | 湛江国联水产开发股份有限公司 | Continuous sampling device for algae detection capable of preventing sample mixing |
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