KR20160034708A - Aromatheraphy serum composition with natural essential and natural hydrosol - Google Patents

Abstract

The present invention relates to a serum composition having an aromatherapeutic effect, comprising: natural essential oil including essential oil of Dendranthema boreale (Makino) Ling ex Kitam., essential oil of Citrus junos Sieb. ex Tanaka, essential oil of Artemisia annua, essential oil of Artemisia princeps Pampanini in Geomundo Island, and linalool; and natural hydrosol including hysdrosol of Dendranthema boreale (Makino) Ling ex Kitam., hysdrosol of Citrus junos Sieb. ex Tanaka, hysdrosol of Artemisia annua, and hydrosol of Artemisia princeps Pampanini in Geomundo Island. The natural hydrosol is added as a solvent. The serum composition in the present invention has a combination of Dendranthema boreale (Makino) Ling ex Kitam. flower scent and Artemisia princeps Pampanini scent, relieves the skin, moisturizes the skin, and has an excellent aromatherapeutic effect.

Description

[0001] The present invention relates to an aromatherapy serum composition comprising natural essential oils and natural hydrogens,

The present invention relates to an aromatherapy serum composition comprising a natural essential oil and a natural hydroazole, and more particularly, to a perfume composition which recreates a unique and fragrant fragrance including natural essential oils and natural hydrogens extracted from natural sources, And exhibits excellent efficacy in antibacterial, anti-atopic, skin-soothing and skin moisturizing.

In order to maintain healthy skin, active ingredients such as cosmetics must be supplied from outside the body. In addition to the addition of active ingredients extracted from natural sources for basic management, the use of these natural materials is increasing rapidly. Especially, cosmetics and herbal extracts are mainly used in cosmetics and cosmetics. Therefore, it is a big field that is never overlooked in the perfume industry. Especially in Europe, especially in France, Which is used in large quantities. Also, researches on the development of natural fragrance have been actively conducted due to the problems caused by the side effects of synthetic fragrance in the world, and recently, the development of functional cosmetic products using fragrance extracted from these natural resources is increasing.

On the other hand, domestic spice industry is estimated to be 350 billion, showing 6% increase every year. By fragrance products, synthetic fragrance accounts for 14.8%, natural fragrance 17.5%, food fragrance 28.5%, and cosmetics fragrance 29.2%. However, the import of natural spices was 865 tons in 2006, with the import of spice ingredients more than 95%, and it was reported that it increased by 8% every year (2006 National Statistical Office). As a result, it is inevitable to develop natural perfumes, focusing on domestic native varieties, and to commercialize natural flavors for import substitution.

On the other hand, a method for collecting a directional substance (essential oil) from a plant tissue includes a compression method, a nonvolatile solvent extraction method, a volatile solvent extraction method, a liquefied gas extraction method, and a steam distillation method.

The compression method is a method of extracting essential oils by squeezing citrus fruits. There is a cell that stores natural essential oil near the surface of the peel. It is a method of treating the unstable fruit flavor unstable at low temperature by the method of squeezing the oil and breaking the cell by squeezing it.

The nonvolatile solvent extraction method is a method of making fragrance by absorbing fragrance of flowers with fat using odorless purified Uji, lard or olive oil. Extracting the fragrant ingredient using high purity alcohol and removing the alcohol gives a fragrance called essence or refined oil.

The volatile solvent extraction method is a method of extracting using a volatile solvent such as nucleic acid, petroleum ester, or benzene. The flower cone obtained by recovering the solvent under reduced pressure is dissolved in high purity alcohol, cooled to about -20 ° C, the insoluble component is removed, and the alcohol is recovered at low temperature. The material thus obtained is called absolute, which is the most refined in essential oils and used for the combination of high-grade fragrances. However, since this process is performed at a lower temperature than the steam distillation process, the solubility is high, and there is a tendency that extra substances such as coloring matters and proteins are included in addition to the fragrance.

The liquefied gas extraction method is a method of extracting perfume materials using liquefied propane, butane, carbon dioxide gas or the like using a special apparatus under high pressure and low temperature. It is possible to obtain high purity perfume as compared with other extraction methods, but it is disadvantageous in that it is expensive.

The steam distillation method is a method of distilling water vapor to distill essential oil, and is classified into water distillation method, water-steam distillation method, and steam distillation method.

The water distillation method is a method of separating water and oil after cooling the steam and essential oil generated by heating the crude oil into water and heating it. Because the raw material is immersed in boiling water, it does not directly contact water vapor. It is suitable for orange flowers and roses that come into contact with water vapor directly.

The water-steam distillation method is a method in which a crude oil is poured into a water vapor in a layer on a grid installed in a distillation section, and the water is heated from below to distill water vapor passing through the sample and a volatile essential oil component separated into water vapor. The plant material is not in contact with water, so it has the advantage of not being overheated and is suitable for some seeds, fruits, peels, some flowers, outposts and leaves that require medium boiling point.

 The steam distillation method is the most common method of oil extraction, in which raw materials are added to the distillation kiln and water vapor is blown from the bottom to collect the essential oil. It is a distillation method which mainly requires low boiling points.

The steam distillation method has the advantage of being safe for skin application because it extracts essential oil with water vapor (water) and has little toxicity. However, although the chemical composition of the essential oil can be changed due to the high temperature, the steam distillation method can extract the essential oil and obtain a hydrosol having the aromatherapy effect.

On the other hand, hydrosol is a term derived from Latin hydro, which means water, and sol, which is called decomposition, and is also called directional distilled water or hydrozoal water. Hydrozoles are obtained in the process of producing essential oils or in the process of distilling plants to obtain only pure hydrogels. Steam distillation of plants results in the production of oil, the essential oil, and the hydrosol, a water component, which contains low levels of essential oils. Hydrosol is a water with aromatherapy value that can be used without difficulty in using high concentration of aroma oil or irritating to sensitive skin and infant. In addition, the hydrosol has antiseptic, germicidal effect, it controls the pH of the skin, gives moisturizing effect to the skin, acts to prevent skin diseases and allergic reaction, and is used as a main ingredient of natural cosmetics.

Aromatherapy, on the other hand, is a combination of aroma (aroma) and therapy (therapy), which refers to aroma therapy and aromatherapy. It is a natural remedy using essential oils extracted from fragrant plants (herbs) for the purpose of health promotion, disease prevention, and beauty. Originally, 100% essential oil is used for treating diseases, skin care, restoring psychological instability. In addition to these pure aromatherapy, cosmetics, air fresheners, fragrances, fragrances, etc. are also used. However, if the concentration of aroma is too high or if you smell for a long time, or if you eat essential oil without doctor's prescription, it may cause side effects and cause an allergic or skin reaction.

Accordingly, the inventors of the present invention have made intensive studies to develop a cosmetic composition having aromatherapy effect while reproducing a unique and fragrant fragrance by blending natural essential oils obtained from natural resources with natural hydrogens in an appropriate ratio, and as a result, they have reached the present invention.

Korean Patent Registration No. 10-0879094 (registered on Jan. 01, 2009) discloses a natural essential oil component containing lavender essential oil and tea tree essential oil, and acne containing a lavender hydrozoal and a tea trihydroazole component obtained in the essential oil extraction A cosmetic composition for improvement is described. Korean Patent Laid-Open No. 10-2012-0106475 (public date: 2012.26.26) contains essential oil of Chamaecyparis obtusa, Chrysanthemum, Ginseng and Rhubarb to effect water supply, reduction of pore size, alleviation of keratin and wrinkles and inhibition of pigmentation And the like.

The present invention relates to a natural hydrosol containing natural essential oils and essential oils containing citric acid essential oil, citron essential oil, ragwort waxy essential oil, Guandon mugwort essential oil and linalool, and acid hydroxides, citrate hydrosol, To provide a serum composition having an aroma therapy effect which is harmonious with the acidity and moxa smell of the country of origin and is effective for antibacterial, anti-atopic, skin sedative and skin moisturizing.

In order to achieve the above object, the present invention provides a natural essential oil containing essential oils of liquorice, safflower essential oil, ragwort wormwood oil, geomundo mugwort oil, and linalool; And a natural hydrazol containing a source of hydrocarbons, a citric acid hydrate, a citric acid hydrate, a citric acid hydrate, a catechin hydrosol, and a genus hydrosol, wherein the natural hydrogens are added as a solvent. to provide.

In the serum composition of the present invention, the natural essential oil preferably contains 5 to 25% by weight of essential oils, 10 to 30% by weight of citron essential oils, 10 to 30% by weight of waxy essential oil, 10 to 30% It is preferable that it contains 15 to 35% by weight of the linalool. The natural essential oil thus prepared is harmonized with the acidity and wormwood smell of the country of origin, and exerts an excellent aromatherapy effect.

In addition, in the serum composition of the present invention, the natural hydroose preferably contains 20 to 40% by weight of a hydroxymale acid, 20 to 40% by weight of a citrate hydrosol, 10 to 30% by weight of a hydrogen peroxide, 10 to 30% by weight. The natural hydroose thus obtained exerts excellent aromatherapy efficacy (anti-stress) and exhibits antibacterial, anti-atopic, skin-soothing and skin moisturizing effects.

Further, in the serum composition of the present invention, it is preferable that the serum composition preferably contains 0.01 to 1% by weight of natural essential oil and 25 to 80% by weight of natural hydrozols. Most of the cosmetic lotion used as a solvent in the production of general cosmetics is distilled water of 100% pure water. The present invention is characterized by using natural hydroesol instead of distilled water or purified water. Accordingly, the fragrance is suitable, the fragrance is not stimulated, and the aromatherapy effect can be exerted. In addition, since the skin soothing effect and skin moisturizing effect are excellent, the skin elasticity enhancement effect can be expected.

On the other hand, serum is a beauty concentrate that supplies nutrition, moisture and gloss to the skin. While the general serums focus on functionality, the serum composition of the present invention is a serum composition focused on aromatherapy efficacy. Thus, the serum composition of the present invention provides a new serum composition that delivers nutritional and moisturizing ingredients to the skin, while exhibiting aromatherapy efficacy.

On the other hand, the Chrysanthemum boreale is also called perennial chrysanthemum (wild chrysanthemum) belonging to the Asteraceae family. The yellow flowers of the country are beautiful and fragrant, and they are also used as chrysanthemums or chrysanthemum tea. It has been mainly used for headache, fever, and decolonization in oriental medicine. Ingredients of the country include essential oil, linarin, glycosides of luteolin, chrysanthemin, It contains chrysanthemaxanthin, polysaccharides, coumarins, yejuhua lactone.

In addition, the essential oil of the country is strong with a blue viscous liquid and has a slightly sweet and warm herb flavor. Its efficacy includes anti-inflammatory, antiseptic, burn, scar, antiallergic, antidepressant, dermatitis, nervous sedative, And the like are known. The major components of the essential oil of the country are α-pinene, camphene, sabinene, β-pinene, o-cymeme, 1,8- But are not limited to, cineole, 1,8-cineole,? -Thujone, chrysanthenone, camphor, borneol, chrysantenyl acetate, sabyl acetate, bornyl acetate,? -longipinene,? -elemene, (E) -carryophyllene,? - Farnesene, germacrene D, delta -cadinene, caryophyllene oxide, chamazulene, and the like.

Citrus junos has been widely used as a medicinal herb for citron, which is an evergreen shrub of Rutaceae, which grows throughout the country, and its fruit shell is called yuzafi (Japanese citrus peel). It is known to have an effect of controlling circulatory diseases and various congestion. In the mainchain gangmok, the citron peeled the food and digested it, and said that it had the effect of digesting food poisoning and decrypting food poisoning. Citron is not only rich in vitamins B1, B2, C and various organic acids, but also contains limonoids, which are known to have excellent anticancer effects, and antioxidants, It is known to be rich in hesperidine known to be effective (Journal of Cardiovascular Pharmacology 22 (2): 225-30, 1993).

In addition, the citron essential oil may be selected from 1R-alpha-pinene, D-Limonene, 3-carene, (+) - 4- carene), 1,3,8-p-menththriene, 1,6-octadien-3-ol, 3- Cyclohexen-1-ol, 1,6,10-dodecatriene, 1H-cycloprop [e] azulene (1H-Cycloprop [e] azulene, p-ment-1-en-8-ol, gamma-Elemene, Ledol, Thymol, And the like.

The Artemisia annua L. is an annual herbaceous plant belonging to the Asteraceae family. It is distributed worldwide as tropical Asian origin, and is wild in many parts of the country. In the oriental medicine, the top part of the mugwort is used as an antipyretic agent, a hemostatic agent, a treatment for skin diseases, and an insecticide.

Caryophyllene, Santolina trien, (-) - germane ((-) - Germacrene), bicyclo [3.1.1] heptane (Bicyclo [3.1. 1-heptane, decahydro-4a-methyl-1-methylene-7- (1-methylethenyl) -naphthalene, Beta-eudesmene, bicyclo [2.2.1] heptan-2-one, e) -beta- Beta-farnesene, beta.-Phellandrene, Artemisia alcohol, Estragole, 1H-cycloprop [e] azulene, Fragrance raw materials, fragrance ingredients such as cosmetics, anti-inflammation used in medicines, and antimicrobial ingredients such as insecticides.

The Geomundo mugwort is abundant in various minerals because of its smooth leaves, sea breeze and seaweed due to its geographical characteristics such as abundant sunshine and granitic soil. Gyomundo mugwort, especially in the human body is unable to synthesize the essential amino acid Histidine (histidine) and 7 contains a large amount of chineol essential oil, antimicrobial activity and antioxidant activity was found to be excellent.

In addition, the Geomundo mugwort essential oil contains Caryophyllene, Pinene, Borneol, Limonene, 1,8-Cineole, Camphene, Copaene), fragrance raw materials, fragrance ingredients such as cosmetics, anti-inflammation used in medicines and insecticides.

In addition, Linalool is one of terpene alcohols having a unique scent as a main component of hedgehog oil. It is a colorless liquid, free of lavender oil, bergamot oil, oil, or as an ester. Lily of the valley, aloe and so on, so it is used in soaps, cosmetics, food fragrances and so on.

On the other hand, in the serum composition of the present invention, the natural essential oils and natural hydrozols can be produced by steam distillation. For example, it can be produced using the method described in Korean Patent No. 10-1105539.

Meanwhile, the serum composition of the present invention may further include components commonly used in the art to which the present invention belongs. For example, glycerin, 1,3-butylene glycol, dipotassium glycyrrhizate, EDTA-2Na, amino coat, methyl paraben, vitamin Vitamin E acetate, KF96 (dimethicone), jojoba oil, macadamia nut oil, Viscolam AT / 64P (sodium acrylate), triethanolamine, cerasome, The biocare HS (hydrolyzed in distilled water, Sclerotium gum (Sclerotium gum)), symldol 68, coheliss, JC complex (botanical extract, Xanthan Gum, Potassium Sorbate, 1,2-Hexanediol, Caprylyl Glycol, Butylene Glycol, Citric Acid, Adenosine, and distilled water (purified water). May be manufactured including any one or more of them.

The present invention relates to a natural hydrosol containing natural essential oils and essential oils containing citric acid essential oil, citron essential oil, ragwort waxy essential oil, Guandon mugwort essential oil and linalool, and acid hydroxides, citrate hydrosol, It is possible to provide a serum composition which is harmonious with the acidity and moxa smell of the country of origin and is effective for antibacterial, anti-atopic, skin sedative and skin moisturizing effects and has an aromatherapy effect.

FIG. 1 shows the result of measurement of brain waves to confirm the effect of the acidic essential oil and citron essential oil on brain activity.
FIG. 2 shows the result of checking the increase of the Upper Alpha index after the EEG reaction test according to the position of EEG measurement of the Korean refined oil and citron essential oil.
FIG. 3 shows the results of confirming the stress reduction effect of the acidic essential oil and citron essential oil.
Fig. 4 shows the result of confirming the antimicrobial activity against the skin fungus of citron essential oil.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[Preparation Example 1: Preparation of natural essential oil]

After preparing the outpost of the Chinese province, the yuza fruit, the outpox and the geomundo wormwood outpour, using the directional essential oil water vapor distillation device (Korean Patent No. 10-1105539) . Thereafter, natural essential oil was prepared by mixing 15 ml of the abovementioned essential oils, 20 ml of the citron essential oil, 20 ml of the above-mentioned ragweed essential oil, 20 ml of the above-mentioned Geomundo mugwort essential oil and 25 ml of linalool.

[Preparation Example 2: preparation of natural hydrogens]

After preparing the outbreak of the Korean prehistoric period, the yuza fruit, the outbreak outpox and the goat mugwort outpour, the acid hydrolyzate, the citrate hydrosol and the goethite wormwood hydrosol were obtained by using the 'directional essential oil water vapor distillation apparatus' (Korean Patent No. 10-1105539) Respectively. Thereafter, 30 ml of the above-mentioned acid hydroxols, 30 ml of the above-mentioned yzohydrozol, 20 ml of the above-mentioned guinea-pomace hydrosol and 20 ml of the above-mentioned Geomundo mugwort hydrosol were mixed to prepare a natural hydro-sol.

[Experimental Example 1: Determination of Influence on the Activity of Brain in Essential Oils and Citron Essential Oil]

In this experiment, central nervous system EEG (EEG) was performed to confirm the effect of the acidic essential oil and citron essential oil prepared in Preparation Example 1 on brain activity.

The central nervous system EEGs were measured and compared before and after each treatment (untreated).

As a result of the experiment, it was confirmed that the brain activity stimulated by Upper alpha, which is the brain activation promoting factor, was activated after the incense of the essential oil of the country and the essential oil of citron when the incense of the citric acid refined oil and the citron essential oil ).

[Experimental Example 2: Confirmation of Stress Reducing Effects of Citric Acid Essential Oil and Citron Essential Oil]

In this Experimental Example, the autonomic nerve response test was performed to confirm the stress reduction effect of the acidic essential oil and citron essential oil prepared in Preparation Example 1.

The heart rate variability (HRV) was measured and compared before and after taking the fragrance of the Korean refined oil and citron essential oil, respectively.

As a result of the experiment, it was confirmed that sympathetic nervous system was reduced after taking the perfume of the national refined oil and citron essential oil before taking the perfume of the national refined oil and the yuzu refined oil (FIG. 3). In other words, it was confirmed that the acid refined oil and the citron oil reduced the stress and gave the comfort.

[Experimental Example 3: Confirmation of effect of inhibiting proliferation of skin supernatant of citrate hydrazole]

example In the Experimental Example, the antibacterial effect of citron hydrate (containing citron essential oil) prepared in Preparation Example 2 was examined to confirm the effect of inhibiting the skin flour proliferation of citron essential oil. Citron essential oil was prepared in the same manner as described in Example 1 above

Staphylococcus aureus and Staphylococcus epidermidis were inoculated into the flask, and citron essential oil was added to the flask at concentrations of 1, 10, 20 and 50 mg / Respectively. The results are shown in Table 4 below.

Citron oil Inhibition Zone Diameter (mm) 50 (mg / ml) 20 (mg / ml) 10 (mg / ml) 1 (mg / ml) Disc 1 Disc 2 Disc 3 Disc 4 Staphylococcus aureus 9.5 5.6 5 -  Staphylococcus epidermidis 10.3 5 4.6 -

As a result, it was confirmed that the antimicrobial activity was increased as the concentration of citron essential oil increased (FIG. 4). From the above results, antimicrobial effect of citrus hydrosol could be expected.

[Experimental Example 4: Analysis of Flavor Components and Content of Essential Oils of Country of Origin]

In this experimental example GC-MASS analysis was performed to analyze the fragrance component and the content of the essential oil of the acid produced in Preparation Example 1 above. Table 2 shows the results of GC-MASS analysis of the essential oil of the country.

RT (min) Compounds Peak area percent (%) Odorant description 939 alpha-pinene 0.85 957 camphene 1.39 fruity 979 sabinene 1.32 982 1-octen-3-ol 0.20 mushroom-like 985 β-pinene 1.05 991 β-mycene 0.87 terpeny 1012 l-phenylindole 0.77 1015 δ-3-carene 0.18 1023 alpha-terpinene 0.40 1028 1,2,3-trimethyl benzene 0.03 1032 o-cymeme 1.83 1038 l-lemonene 0.74 1043 1,8-cineole 5.04 camphoraceous 1049 β-ocimene 0.15 1065 gamma-terpinene 0.82 citrus 1092 alpha-terpinolene 0.25 pine 1096 α-dimethyl styrene 0.07 1130 β-thujone 2.97 cedarleaf 1135 chrysanthenone 1.98 1149 스 ylyl alcohol 0.17 1154 (Z) -sabinol 0.36 1158 (E) -pinocarveol 0.36 1166 camphor 5.96 1177 pinocarvone 0.55 1183 camphene 0.83 1191 borneol 5.51 pine 1194 4-terpineol 0.79 1207 alpha-terpineol 0.16 1209 myrtenol 0.57 1239 chrysantenyl acetate 1.45 1256 carvone 0.19 herbal 1225 p-cumic aldehyde 0.30 1266 chrysanthenyl acetate 1.28 1276 camphene 0.11 1285 geranyl acetate 0.11 rose like 1297 부닐 미산 4.71 1298 bornyl acetate 3.14 1304 carvacrol 0.12 1323 공용 고 0.10 1333 myrtenyl acetate 0.19 1356 alpha-terpenyl acetate 0.47 1365 (Z) -carvyl acetate 0.31 1372 alpha-longipinene 1.06 1393 α-copaene 0.48 1404 β-elemene 1.91 citrus 1430 보러 이소 부열 0.14 1446 (E) -caryophyllene 4.74 1459 β-farnesene 2.19 1480 α-humulene 0.57 1508 germacrene D 8.91 1515 valencene 0.86 1519 γ-elemene 0.97 1534 δ-cadinene 1.41 1549 alpha-bisabolene 0.75 1566 nerolidol 0.25 1593 (E) -longipinocarveol 0.37 1603 spathulenol 0.41 1612 caryophyllene oxide 1.30 1759 chamazulene 4.97

As a result of analysis, it was found that the essential oil of the country of origin is composed of α-pinene, camphene, sabinene, β-pinene, o-cymeme, But are not limited to, 1,8-cineole,? -Thujone, chrysanthenone, camphor, borneol, chrysantenyl acetate, sabinyl acetate, bornyl acetate,? -longipinene,? -elemene, (E) -carryophyllene,? -paradine, It has been reported that it contains β-farnesene, germacrene D, δ-cadinene, caryophyllene oxide, and chamazulene. Here, chanazulene, which has an antiinflammatory and strong anti-inflammatory action, showed a high content of 4.97%, and an ester component accounted for more than 11%, so that an antiallergic action could be expected.

[Experimental Example 5: Analysis of Flavor Components and Content of Citron Essential Oil]

In this experimental example GC-MASS analysis of citron essential oil was carried out to analyze the fragrance components and content of the citron hydrosol (containing citron essential oil) prepared in Preparation Example 2 above. Citron essential oil was prepared in the same manner as described in Example 1 above. Table 3 below shows the GC-MASS analysis results of citron oil.

RT (min) Compounds Peak area percent (%) Odorant description 3.870 1R-alpha-Pinene 1.2202 6.283 beta-Pinene 0.1165 Pine-like 8.451 beta-Phellandrene 0.1133 Peppery, Fresh 10.400 D-Limonene 30.6517 Sour, Lemon-like 12.427 3-carene 19.7641 13.015 (+) - 4-carene 4.4449 16.611 1-phenyl-1-butene 0.9260 16.687 1,3,8-p-Menthatriene 0.3237 Citrusy, Oily 17.451 (+) - 4-carene 0.6942 18.173 Decanal 0.4357 Sweet, Sour 19.671 1,6-Octadien-3-ol 11.2949 20.144 Cyclohexane 0.4328 20.522 Caryophyllene 0.8657 20.680 3-Cyclohexen-1-ol 2.4095 20.855 bicyclo [2.2.1] hept-2-ene 0.4514 21.309 1,3,5-Heptatriene, 0.4507 21.385 cis-beta-terpineol 0.6802 22.015 alpha-Caryophyllene 0.3239 22.289 1,6,10-Dodecatriene 1.9038 22.527 Bicyclo [2.2.1] heptane 0.4607 22.708 1H-Cycloprop [e] azulene 1.6789 22.935 p-menth-1-en-8-ol 4.9166 23.524 gamma-Elemene 0.8780 Yuzu-like, Sweet 23.536 1,3,6-Heptatriene 0.8780 24.072 (+) - Epi-bicyclosesquiphellandrene 0.8530 25.156 2,6-Octadien-1-ol 0.2523 25.494 gamma-Elemene 0.2999 Yuzu-like, Sweet 26.176 2,6-Octadien-1-ol 0.6266 29.038 beta-Panasinsene 0.1969 29.359 Epiglobulol 0.2585 29.446 (z) -4-decen-1-ol 0.4582 30.151 1,3-Dimethyl-5-ethyladamantane 0.6231 30.559 Ledol 1.2603 Sweet 31.299 1H-Indene 0.1584 32.308 1H-Cyclopropa [a] naphthalene 0.3441 32.663 Thymol 2.9055 33.346 alpha-Cadinol 0.3314 44.508 n-Hexadecanoic acid 0.8710

As a result of the analysis, the essential oil of citron was 1R-alpha-pinene, D-Limonene, 3-carene, (+) - 4- -carene, 1,3,8-p-menththriene, 1,6-octadien-3-ol, 3 3-Cyclohexen-1-ol, 1,6,10-Dodecatriene, 1H-cycloprop [e] Cycloprop [e] azulene, p-ment-1-en-8-ol, gamma-Elemene, Ledol, Thymol ), And so on.

[Experimental Example 6: Analysis of fragrance component and content of mugwort refined oil]

In this experimental example GC-MASS analysis was carried out to analyze the fragrance component and the content of the crude wolfberry milk prepared in Preparation Example 1. Table 4 below shows the results of GC-MASS analysis of the mugwort milk essential oil, and Table 5 shows the flavor or efficacy of the components contained in the mugwort milk essential oil.

PK RT (min) Compound name Area Pct
(%) One 4.7642 Ethyl ether 0.1673 2 7.7881 Santolinatriene 0.3166 3 8.4705 1S-alpha.-Pinene 0.2229 4 8.8941 1,3,6-Heptatriene 0.0881 5 9.253 Camphene 0.7250 6 10.4884 beta.-Phellandrene 0.9819 7 11.3827 beta.-Myrcene 0.3230 8 13.1064 Santolina trien 5.0748 9 14.0536 1,4-Cyclohexadiene 0.2436 10 16.6303 2-Butyl-1,2-azaborolidine 0.2058 11 16.9951 2,6-Octadienal 0.2800 12 17.7775 Artemisia alcohol 1.4212 13 18.2423 Copaene 0.2990 14 18.5011 1,6-Octadien-3-ol 0.3922 15 19.2365 Bicyclo [2.2.1] heptan-2-one 2.7029 16 20.0837 Bicyclo [2.2.1] heptan-3-one 0.1747 17 21.172 Caryophyllene 1.3872 18 21.7603 Estragole 0.8510 19 22.2251 1,5,7-Octatrien-3-ol 0.3733 20 22.7369 (e) - [beta] -FARNESENE 0.4020 21 23.6665 1,5-Cyclodecadiene 0.0235 22 24.1136 Tricyclo [5.4.0.0 (2,8)] undec-9-ene 0.5457 23 24.3371 (-) - Germacrene 0.4647 24 24.6371 decahydro-4a-methyl-1-methylene-7- (1-methylethenyl) -Naphthalene 0.7555 25 25.0548 alpha.-Cubebene 1.7440 26 25.5902  (-) - Germacrene 0.2407 27 25.9079 1H-Cycloprop [e] azulene 0.0963 28 26.5256 α-Cedrene 0.1254 29 27.1198 Caryophyllene 6.2754 30 27.4492 Propanoic acid 0.8047 31 27.8081 Naphthalene 0.0849 32 28.2493 (e) - [beta] -FARNESENE 2.2918 33 28.5847 Naphthalene 0.1101 34 29.673 D-Germacrene 4.7237 35 29.9378 beta-Eudesmene 4.0560 36 33.4205 alpha.-Farnesene 0.2661 37 34.8501 Caryophyllene oxide 1.7783

ingredient Incense or efficacy Caryophyllene Air purification (phytoncide), medicinal Santolinatrien Inflammation, pain, atopy, arthritis, antibacterial activity (-) - Germacrene Antimicrobial, insecticide Bicyclo [3.1.1] heptane Food flavor, perfume, antibacterial, lobular agent, antitumor, seasoning, insecticide decahydro-4a-methyl-1-methylene-7- (1-methylethenyl) -Naphthalene Cosmetics fragrances, food fragrances, antimicrobial agents, paint raw materials, air purification (phytoncide), anti-inflammation beta-Eudesmene Conflict, Tonic, Anti-inflammatory, itching Bicyclo [2.2.1] heptan-2-one Moxibustion odor, medicines, spices, insecticides and pesticides (e) - [beta] -Farnesene Herb fragrance, blueprint smell, perfume, fragrance, insect pheromone, aphid remover beta.-Phellandrene Odor reducing agent, fragrance raw material Artemisia alcohol Mugwort, anti-malaria, anthelmintic, anticancer, insecticidal action Estragole Herbal fragrances, cosmetics fragrances, fragrances 1H-Cycloprop [e] azulene Blue anti-inflammatory, antibacterial, anti-inflammatory

Caryophyllene, Santolina trien, (-) - germane ((-) - Germacrene), bicyclo [3.1.1] heptane (Bicyclo [3.1 Methyl-1-methylene-7- (1-methylethenyl) -Naphthalene, Beta-eudesmene, bicyclo [2.2.1] heptan-2-one, e) -beta-phenesene (e) -beta-farnesene, beta.-Phellandrene, Artemisia alcohol, Estragole, 1H-cycloprop [e] azulene ), Fragrance raw materials, fragrance ingredients such as cosmetics, anti-inflammation used for medicines, and insecticides.

[Experimental Example 7: Analysis of Flavor Components and Content of Mugwort mugwort essential oil]

In this experimental example GC-MASS analysis of Geomundo mugwort refined oil was carried out to analyze the fragrance components and contents of the Geomundo mugwort hydrosol (containing Geomundo mugwort essential oil) prepared in Preparation Example 2 above. Geomundo mugwort essential oil was prepared by the same method as described in Preparation Example 1 above. Table 6 below shows the results of GC-MASS analysis of Geomundo mugwort essential oil, and Table 7 below shows the flavor or potency of the components contained in Geomundo mugwort essential oil.

No. RT (min) Compound name Area Pct
(%) One 4.952 2-ethyl-Furan 0.0367 2 6.964 Nonane 0.0845 3 8.470 1R-.alpha.-Pinene 11.135 4 8.935 Camphene 3.7927 5 9.759 beta.-Pinene 6.4164 6 10.364 beta.-Myrcene 1.3326 7 10.947 (+) - 2-Carene 0.7173 8 11.441 Limonene 6.5936 9 11.612 1,8-Cineole 4.4015 10 12.741 1-methyl-4- (1-methylethylidene) -Cyclohexene 0.9203 11 14.118 2,4,6-Octatriene 0.7748 12 14.647 1-methyl-4- (1-methylethenyl) -Benzene 0.7145 13 14.959 1,4-Cyclohexadiene 0.6234 14 16,000 3-Cyclopentene-1-acetaldehyde 0.4226 15 16.589 3-Carene 0.3455 16 16.759 Camphor 0.5936 17 17.195 1,4-Cyclohexadiene 0.2335 18 17.854 2 (10) -Pinene-3-one 0.6306 19 18.501 3-Cyclohexen-1-ol 2.2072 20 19.118 Acetic acid 0.7494 21 19.389 3-Cyclohexene-1-methanol 2.9196 22 19.989 1,3,8-p-Menthatriene 1.4766 23 20.683 L (-) - Borneol 8.5223 24 21.601 Caryophyllene 15.3488 25 22.972 1,4,7, -Cycloundecatriene 5.8548 26 23.537 gamma-cadinene 1.2705 27 23.960 Bicyclo [4.4.0] dec-1-ene 2.2212 28 24.201 Copaene 3.2626 29 25.243 γ-Muurolene 2.6628 30 27.296 1H-Benzocycloheptene 0.1713 31 28.290 alpha.-Calacorene 0.1101 32 28.943 Bicyclo [3.3.1] non-3-en-2-ol 0.2391 33 29.449 1H-Benzocycloheptene 0.0887 34 29.761 trans-Z-.alpha.-Bisabolene epoxide 0.0501 35 30.349 Caryophyllene oxide 0.5506 36 32.043 1H-Cycloprop [e] azulene 0.1459 37 32.738 Humulen- (v1) 0.0491 38 33.2146 1H-Cycloprop [e] azulen-7-ol 0.1149 39 33.8618 1H-Indene 0.1748 41 34.6266 Naphthalene 0.3847 42 37.4975 3-Methylene-bicyclo [3.2.1] oct-6-en-8-ol 0.1411

ingredient Incense or efficacy Caryophyllene Air purification (phytoncide), medicinal Pinene Air purification (phytoncide), antibacterial, anti-inflammation Borneol Pepper flavor, poultice, cosmetic perfume Limonene Cosmetics fragrances, food fragrances, antimicrobial agents, paint raw materials, air purification (phytoncide), anti-inflammation 1,8-Cineole Fresh camphor odor, antibacterial, respiratory disease prevention, inflammation, fatigue, pain relief cosmetic perfume, perfume, food fragrance, oral cleaning, cough suppression, Camphene Moxibustion odor, medicines, spices, insecticides and pesticides Copaene Perfume, cosmetic perfume

As a result of analysis, Geomundo mugwort refined oil was classified as Caryophyllene, Pinene, Borneol, Limonene, 1,8-Cineole, Camphene, (Copaene), fragrance raw materials, fragrance ingredients such as cosmetics, antimicrobial ingredients such as anti-inflammation and insecticide used for medicines.

[Experimental Example 8: Confirmation of Skin Efficacy of Essential Oils and Citron Essential Oils]

In this Experimental Example, the skin efficacy of the citric acid essential oil prepared in Preparation Example 1 and the citric acid hydrate prepared in Preparation Example 2 (containing citron essential oil) was examined.

Citron essential oil was prepared by the same method as described in Preparation Example 1 above.

Immediately after the pre-measurement, the control was used as a control, and 100 ml of jojoba oil, 5 ml of acidic essential oil + 95 ml of jojoba oil, 5 ml of citron essential oil + 95 ml of jojoba oil were applied to each of the test subjects, , Melanin (whitening), erythema, skin pH, and elasticity. The skin moisture content (Moisture) was measured by Corneometer CM825, the Melanin Index and the erythema index were measured with Mexameter MX18, the skin pH was measured with Skin-pH-meter PH905, and the elasticity was measured with Custometer MPA580. The results are shown in Table 8 below.

Measure natural
essential oil
gender Immediately after cleaning A
(Before measurement) Measurement time B Average car
(BA) efficacy After 1 hour
After 3 hours After 5 hours medium ¹⁾ Moisturizing
(AU) Jojoba oil 100% south 47.50 47.50 59.70 57.60 54.93

10.2 increase
(good) female 35.10 47.30 50.00 47.00 48.10 Average 41.30 47.40 54.85 52.30 51.52 Citron oil 5% + Jojoba oil 95% south 43.70 55.50 58.30 65.30 59.70

13.28 increase
(Great) female 39.50 49.95 49.40 50.80 50.05 Average 41.60 52.73 53.85 58.05 54.88 5% of the country's essential oil + 95% of the jojoba oil south 42.70 58.00 58.10 57.10 57.73

13.1 increase
(Great) female 39.35 49.95 49.15 52.35 50.48 Average 41.03 53.98 53.63 54.73 54.11 ²⁾ melanin index
(MI) Jojoba oil 100% south 193.00 198.00 221.00 224.00 214.33

7.5 increase
(none) female 133.00 128.00 128.00 124.00 126.67 Average 163.00 163.00 174.50 174.00 170.50 Citron oil 5% + Jojoba oil 95% south 193.00 199.00 190.00 179.00 189.33

-4.0 decrease
(good) female 138.50 137.50 128.50 136.50 134.17 Average 165.75 168.25 159.25 157.75 161.75 5% of the country's essential oil + 95% of the jojoba oil south 178.00 179.00 167.00 168.00 171.33

-4.5 decrease
(good) female 125.50 126.00 123.00 120.50 123.17 Average 151.75 152.50 145.00 144.25 147.25 ³⁾ Erythema
Indices
(EI) Jojoba oil 100% south 411.00 362.00 381.00 369.00 370.67

-30.3 decrease
(Extremely
Great) female 190.50 187.50 157.50 165.50 170.17 Average 300.75 274.75 269.25 267.25 270.42 Citron oil 5% + Jojoba oil 95% south 328.00 291.00 336.00 321.00 316.00

-12.5 decrease
(Great) female 180.00 179.50 170.00 151.50 167.00 Average 254.00 235.25 253.00 5.97 241.50 5% of the country's essential oil + 95% of the jojoba oil south 330.00 277.00 328.00 302.00 302.33

-23.3 decrease
(Extremely
Great) female 199.50 198.00 172.00 172.00 180.67 Average 264.75 237.50 250.00 237.00 241.50

Skin pH Jojoba oil 100% south 5.55 5.79 5.52 5.78 5.70

0.03 slightly
increase female 6.06 5.71 6.06 6.16 5.98 Average 5.81 5.75 5.79 5.97 5.84 Citron oil 5% + Jojoba oil 95% south 5.09 5.45 5.40 5.30 5.38

0.09 slightly
increase female 5.91 5.66 5.84 5.90 5.80 Average 5.50 5.56 5.62 5.60 5.59 5% of the country's essential oil + 95% of the jojoba oil south 4.65 5.04 5.13 4.77 4.98

0.13 slightly
increase female 5.65 5.50 5.35 5.87 5.57 Average 5.15 5.27 5.24 5.32 5.28 ⁴⁾ Elasticity
(AU) Jojoba oil 100% south 0.8945 0.902 0.9062 0.9154 0.91

0.03 Extremely
Minimal female 0.9041 0.9352 0.9464 0.9377 0.94 Average 0.90 0.92 0.93 0.93 0.93 Citron oil 5% + Jojoba oil 95% south 0.8872 0.8926 0.9247 0.9318 0.92

0.20 Extremely
Minimal female 0.9010 0.9034 0.9108 0.9129 0.91 Average 0.89 0.90 0.92 0.92 0.91 5% of the country's essential oil + 95% of the jojoba oil south 0.8965 0.9191 0.9098 0.9157 0.91

0.01 Extremely
Minimal female 0.8802 0.9032 0.8731 0.9046 0.89 Average 0.89 0.91 0.89 0.91 0.90

¹⁾ Moisture: Arbitrary unit = AU ²⁾ Melanin index: MI = Melanin index ³⁾ Erythema index: EI = Erythema index ⁴⁾ Elasticity: AU = Arbitrary unit

As a result of the experiment, it was found that the essential oils of sunflower oil and citron oil were excellent in skin moisturizing effect and melanin reduction (whitening) effect, and also had redness reduction effect.

[Experimental Example 9: Confirmation of skin efficacy of mugwort refined oil and mugwort mugwort refined oil]

In this Experimental Example, the skin efficacy of the mugwort milk essential oil prepared in Preparation Example 1 and the mugwort mugwort hydrosol prepared in Preparation Example 2 (containing Geomundo mugwort essential oil) was examined.

Geomundo mugwort essential oil was prepared by the same method as described in Preparation Example 1 above.

As a control group, 100 ml of grape seed oil, 5 ml of mugwort milk essential oil + 95 ml of grape seed oil, 5 ml of geomundo mugwort oil, and 95 ml of grape seed oil were used as a control group. (Water content (moisturizing), elasticity, melanin (whitening), erythema and skin pH) of the skin over time for a total of 6 hours at intervals of 1 hour. The skin moisture content (Moisture) was measured by Corneometer CM825, the Melanin Index and the erythema index were measured with Mexameter MX18, the skin pH was measured with Skin-pH-meter PH905, and the elasticity was measured with Custometer MPA580. The results are shown in Table 9 below.

¹⁾ Moisture: Arbitrary unit = AU ²⁾ Melanin index: MI = Melanin index ³⁾ Erythema index: EI = Erythema index ⁴⁾ Elasticity: AU = Arbitrary unit

Experimental results showed that mugwort essential oil had excellent skin moisturizing effect, melanin reduction (whitening) effect and erythema reduction effect.

In addition, it was found that Geomundo mugwass essential oil has excellent skin moisturizing effect, melanin reduction (whitening) effect and erythema reduction effect.

[Example 1: Preparation of an aromatherapy serum composition containing natural essential oils and natural hydrozols]

In this Example, an aromatherapy serum composition comprising the natural essential oil prepared in Preparation Example 1 and the natural hydroazole prepared in Preparation Example 2 was prepared.

As shown in Table 10, purified water was mixed with distilled water and natural hydrazole, 0.01% of natural essential oil was added, and the raw materials were mixed in the order of A to F to prepare a serum composition.

fair
division Raw material name Ingredients (INCI NAME) content
(g) A Glycerin Glycerin 6.0 1,3 BG 1,3-Butylene glycol 4.0 Lipo DPG DIPOTASSIUM GLYCYRRHIZATE < RTI ID = 0.0 > 0.1 EDTA-2Na Disodium EDTA (DISODIUM EDTA) 0.02 Aminocoat Betaine (BETAINE) 1.0 Purified Water 1 30% by weight of citric acid hydrosol + 30% by weight of citrate hydrosol + 20% by weight of cocarose hydrosol + 20% 30.0 Purified water 2 Purified water 30.0 B Carbopol-941 CARBOMER 0.2 Purified water Purified water 6.0 C antiseptic METHYLPARABEN - Vitamin-E TOCOPHERYL ACETATE 0.2 KF96 DIMETHICONE 1.0 Functional Oil 1 Jojoba oil 1.0 Functional Oil 2 Macadamia seed oil 1.0 D TEA TRIETHANOLAMINE 0.16 Purified water Purified water 2.0 E Spices Natural essential oil (15% by weight of the country's essential oil + 20% by weight of citron essential oil + 20% by weight of mugwort-wax essential oil + 20% 0.01 F 5% Cerasome Hydrogenated lecithin, glycerin, ceramide, octyldodecanol, 1,2-hexanediol, caprylyl glycol nano-mixed solution 5% 3.0 Functional extract Rye seed extract 3.0. Complex plant extract Pine tree (leaf), centipedes (outpost), leggings (outpost) 5.0 Adenosine (%) 2% Adenosine 1.0 Purified water Purified water 5.31 Total amount 100.0

[Example 2: Preparation of an aromatherapy serum composition containing natural essential oils and natural hydrozols]

In this Example, an aromatherapy serum composition containing the natural essential oil prepared in Preparation Example 1 and the natural hydrazole prepared in Preparation Example 2 was prepared.

As shown in Table 11 below, natural hydrogens were added to distilled water instead of distilled water, 0.2% of natural essential oil was added, and the raw materials were mixed in the order of A to F steps to prepare a serum composition.

fair
division Raw material name Ingredients (INCI NAME) Example 1 A Glycerin Glycerin 6.0 1,3 BG 1,3-Butylene glycol 4.0 Lipo DPG DIPOTASSIUM GLYCYRRHIZATE < RTI ID = 0.0 > 0.1 EDTA-2Na Disodium EDTA (DISODIUM EDTA) 0.02 Aminocoa Betaine (BETAINE) 1.0 Purified Water 1 30% by weight of citric acid hydrosol + 30% by weight of citrate hydrosol + 20% by weight of cocarose hydrosol + 20% 60.0 Purified water 2 Purified water - B Carbopol-941 CARBOMER 0.2 Purified water 30% by weight of citric acid hydrosol + 30% by weight of citrate hydrosol + 20% by weight of cocarose hydrosol + 20% 6.0 C antiseptic METHYLPARABEN - Vitamin-E TOCOPHERYL ACETATE 0.5 KF96 DIMETHICONE 1.0 Functional Oil 1 Jojoba oil 1.0 Functional Oil 2 Macadamia seed oil 1.0 D TEA TRIETHANOLAMINE 0.16 Purified water 30% by weight of citric acid hydrosol + 30% by weight of citrate hydrosol + 20% by weight of cocarose hydrosol + 20% 2.56 E Spices Natural essential oil (15% by weight of the country's essential oil + 20% by weight of citron essential oil + 20% by weight of mugwort-wax essential oil + 20% 0.2 F 5% Cerasome Hydrogenated lecithin, glycerin, ceramide, octyldodecanol, 1,2-hexanediol, caprylyl glycol nano-mixed solution 5% 3.0 Coheliss Rye seed extract 3.0. Complex plant extract Pine tree (leaf), centipedes (outpost), leggings (outpost) 5.0 Adenosine (%) 2% Adenosine 1.0 Purified water 30% by weight of citric acid hydrosol + 30% by weight of citrate hydrosol + 20% by weight of cocarose hydrosol + 20% 4.26 Total amount 100.0

[Comparative Example 1: Preparation of a general serum composition]

In this comparative example, a general serum not containing natural essential oils and natural hydrozols was prepared.

As shown in Table 12 below, only purified water was added, and the combination fragrance was added instead of natural essential oil, and the mixture was mixed in the order of A to F to prepare a serum composition.

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division Raw material name Ingredients (INCI NAME) Comparative Example 1 A Glycerin Glycerin 6.0 1,3 BG 1,3-Butylene glycol 4.0 Lipo DPG DIPOTASSIUM GLYCYRRHIZATE < RTI ID = 0.0 > 0.1 EDTA-2Na Disodium EDTA (DISODIUM EDTA) 0.02 Aminocoa Betaine (BETAINE) 1.0 Purified Water 1 Natural hydrosol - Purified water 2 Purified water 60.0 B Carbopol-941 CARBOMER 0.2 Purified water Purified water 6.0 C antiseptic METHYLPARABEN 0.1 Vitamin-E TOCOPHERYL ACETATE 0.5 KF96 DIMETHICONE 1.0 Functional Oil 1 Jojoba oil 1.0 Functional Oil 2 Macadamia seed oil 1.0 D TEA TRIETHANOLAMINE 0.16 Purified water Purified water 2.56 E Spices Combination fragrance 0.2 F 5% Cerasome Hydrogenated lecithin, glycerin, ceramide, octyldodecanol, 1,2-hexanediol, caprylyl glycol nano-mixed solution 5% 3.0 Coheliss Rye seed extract 3.0. Complex plant extract Pine tree (leaf), centipedes (outpost), leggings (outpost) 5.0 Adenosine (%) 2% adenosine 1.0 Purified water Purified water 4.16 Total amount 100.0

[Experimental Example 9: feeling test of serum composition]

In this experimental example, the feeling test of each of the serum compositions prepared in Examples 1 to 2 and Comparative Example 1 was performed.

The feeling test was a physical property test, measuring the appearance, oiliness, absorbency, freshness, moisturizing, stickiness, flexibility, flavor, formability and overall feel as pH measurement and sensory test. The rating was not good: 1 to very good: 5. The results are shown in Table 13 below.

Item Comparative Example 1 Example 1 Example 2 <Property Test> pH 4.56 (23 C) 4.97 (23 C) 5.02 (23 DEG C) <Sensory Test> result grade result grade result grade Exterior Milky liquid 5 Milky liquid 5 Milky liquid 5 Inheritance Low 5 Low 5 moderation 3 Absorption power Slow One speed 5 usually
(A lot of water) 3 Total ductility Insufficient One moderation 3 Great 5 Bearing capacity Low One good 5 moderation 3 Sticky Too many One moderation 5 Low 3 flexibility Inadequate One moderation 3 good 5 Fragrance Poor (soap odor) One moderation 5 Strong 3 Formulation status Becoming liquid One Liquid is suitable 5 Liquid is thin 3 Overall
Feeling Slightly
There is a smell of soap 15 Country flower fragrance
Mugwort 41 Strong smell of wormwood 37

As a result of the test, the serum compositions prepared in Examples 1 and 2 were measured with excellent scores.

From the above results, it has been confirmed that the serum composition containing the natural essential oils extracted from the Korean provinces, the Japanese white mugwort and the Korean mugwort, and the natural hydrogens extracted from the yuzu, citron, mugwort and mugwort mugwort are excellent in absorption power, moisture resistance and flexibility I could.

Claims (4)

Natural essential oil containing linalool, essential oil of korean oil refinery, citron essential oil, citron essential oil,
A natural hydrosol containing acid hydroxides, acid hydroxides, citric hydrogens, citrate hydrogens,
Wherein the natural hydro-sol is added as a solvent.
The method according to claim 1,
The natural essential oil,
Characterized in that it contains 5 to 25% by weight of essential oil of the province, 10 to 30% by weight of citron essential oil, 10 to 30% by weight of essential oil of mugwort wax, 10 to 30% Therapeutic serum composition.
The method according to claim 1,
The natural hydro-
Characterized in that it contains 20 to 40% by weight of a citric acid hydrosol, 20 to 40% by weight of citrus hydrosol, 10 to 30% by weight of a propolis hydrosol and 10 to 30% Composition.
The method according to claim 1,
The above-
0.01 to 1% by weight of natural essential oils and 25 to 80% by weight of natural hydrozols.

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