KR20160029918A - Method for improving the resistance to the drought or salt stress using CaLOX1 in plants - Google Patents

Abstract

The present invention relates to a method for enhancing resistance against desiccation or salt stress in plants. More specifically, the present invention relates to a method for enhancing resistance against desiccation or salt stress in plants, by regulating expression of capsicum lipoxygenase 1 (CaLOX1) protein. A transgenic plant (CaLOX1-OX) in which CaLOX1 of the present invention is over-expressed has shown effects of reducing sensitivity for abscisic acid and enhancing resistance against desiccation or salt stress. Thus the method of the present invention can be useful for improving crops that men can use.

Description

[0001] The present invention relates to a method for improving dryness or salt stress resistance of plants using CaLOX1,

More particularly, the present invention relates to a method for improving the resistance to drying or salt stress of a plant by controlling the expression of CaLOXl (Capsicum Lipoxygenase 1) protein .

Environmental stresses, such as drought and high salinity, directly affect grain growth and production. Especially, as the desertification progresses today, water shortages cause big problems in agriculture and the environment. Therefore, it is necessary to develop plants that can survive in a dry environment even when water is used less. When these techniques are developed and applied to crops, agricultural production is expected to increase greatly. Especially in dry areas, plants with improved resistance to dryness, that is, plants capable of lowering the transpiration rate, are favorable to survival, And may be useful for environmental purification in areas where the environment is very dry.

Plants, on the other hand, have been developing physiological, biochemical and molecular defense mechanisms to cope with the harmful effects of stress because they are frequently exposed to various environmental stresses such as drought, salt, cold, heat, abscisic acid: ABA) signal transduction regulation allows plants to overcome these stresses. It has been reported that plants pretreated with abscisic acid and stressed are resistant to stress compared to plants that do not produce stress, while mutant plants that fail to produce abscisic acid or fail to respond to abscisic acid are known to be stress-free. Therefore, it is expected that the use of proteins involved in the reaction of abscisic acid will enable to develop plants with improved resistance to environmental stress.

Accordingly, a method of increasing the drying or salt resistance of plants has been a major subject of study, and studies have been conducted on the use of proteins involved in the reaction of abscisic acid (Korean Patent Publication No. 10-2010-0040789) It is not enough.

Disclosure of the Invention The present invention was conceived to solve such problems as described above. The present inventors have confirmed that the effect of reducing the sensitivity of abscisic acid and the resistance to drying or salt stress of the transgenic plant ( CaLOX1- OX) overexpressing CaLOX1 , Thereby completing the present invention.

Accordingly, an object of the present invention is to provide a method for promoting drying or salt stress resistance of a plant comprising transforming a gene encoding a CaLOX1 protein into a plant, and overexpressing the CaLOX1 protein in the transformed plant.

It is another object of the present invention to provide a transgenic plant having improved drying or salt stress resistance by the above method.

However, the technical problem to be solved by the present invention is not limited to the above-mentioned problems, and other matters not mentioned can be clearly understood by those skilled in the art from the following description.

In order to accomplish the object of the present invention, the present invention provides a method for producing a plant, comprising the step of transforming a gene encoding a CaLOX1 protein into a plant, and a step of overexpressing the CaLOX1 protein in the transformed plant, Thereby providing a method of enhancing stress resistance.

In one embodiment of the present invention, the CaLOX1 protein may be composed of the amino acid sequence of SEQ ID NO: 1.

In another embodiment of the present invention, the gene coding for the CaLOX1 protein may comprise the nucleotide sequence of SEQ ID NO: 2.

The present invention provides a transgenic plant improved in drying or salt stress resistance by the above method.

In one embodiment of the present invention, the transgenic plant may be Arabidopsis .

The present invention provides a method of screening a plant for drying or salt stress resistance enhancer, comprising the step of treating the candidate substance and then measuring the overexpression of the CaLOX1 protein.

The method for enhancing dryness or salt stress resistance of a plant according to the present invention includes overexpression of CaLOX1 protein. The method of the present invention is characterized in that the CaLOX1 overexpressed transgenic plant ( CaLOX1- OX) has an abscisic acid sensitivity reduction effect, As a result, the CaLOX1 expression is expected to be useful for the improvement of human crops.

FIG. 1 shows the results of qRT-PCR for the change in the amount of CaLOX1 expression under the conditions of (A) abscisic acid (ABA), (B) drying, and (C) salt stress.
Fig. 2 shows the root lengths obtained by ABA treatment before germination (A) or ABA treatment after germination (A) between the CaLOX1 overexpressed transgenic Arabidopsis thaliana ( CaLOX1- OX # 12, # 16) and the wild type (WT) And (C) the result of quantifying root length by ABA treatment after germination.
FIG. 3 shows (A) root lengths of (A) root length between transgenic Arabidopsis plants overexpressing CaLOX1 over osmotic stress conditions, (B) root length, (C) germination rate, And (D) green cotyledon.
FIG. 4 is a graph showing the ratio of (A) germination rate, (B) green cotyledon percentage and (C) percentage of green cotyledons between the transgenic Arabidopsis plants overexpressing CaLOX1 and the wild type control under salt stress conditions Observation results.
FIG. 5 shows the results of visual observation of discoloration of (A) leaves after germination by NaCl treatment between the transgenic Arabidopsis thaliana plants overexpressing CaLOX1 under salt stress conditions, (B) chlorophyll concentration, (C ), And (D) the result of quantifying the root length.
FIG. 6 shows (B) the survival rate, (C) fresh weight, and (C) the yellowing phenomenon (A) between the transformed Arabidopsis thaliana plants overexpressing CaLOX1 in the vegetative state and the wild- (D) concentration of chlorophyll, and (F) brown spots.
7 is a result of comparing the expression patterns of genes DREB2A, RD20, RD29a, RD29b, P5CS between the salt stress conditions, the seedlings state CaLOX1 the transgenic Arabidopsis thaliana plants with the wild type control group.
Figure 8 shows the survival rate, (B) the water loss, (C) the degree of air release, (D) the MDA concentration, and (E) the survival rate between the transgenic Arabidopsis plants overexpressing CaLOX1 and the wild- This is the result of visual observation of brown spots.
9 is a result of a comparison COR15A, DREB2A, RD20, RD29a, RD29b gene expression profile between the stress in a dry condition, the CaLOX1 the transgenic Arabidopsis thaliana plants with the wild type control group.

The present inventors confirmed the effect of increasing the expression of CaLOX1 (Capsicum Lipoxygenase 1) in the dry or salt stress condition and the effect of decreasing the sensitivity of abscisic acid sensitivity and the resistance to drying or salt stress in transgenic Arabidopsis thaliana overexpressing CaLOX1 , Thus completing the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides a method for promoting drying or salt stress resistance of a plant comprising transforming a gene encoding a CaLOX1 protein into a plant and overexpressing the CaLOX1 protein in the transformed plant.

CaLOX1 overexpressed in the present invention is a lipoxygenase protein derived from red pepper. Lipoxygenase proteins are found in plants, animals and molds, and are a class of enzymes known to function in cells

Lipoxygenase acts as an enzyme that catalyzes the oxidation of lipoxygenase and unsaturated fatty acid in most plants and is known to be involved in resistance to insects, response to wounds, aging, and resistance to plant diseases. However, There is no report on stress resistance. Accordingly, the present invention is characterized by overexpression of the CaLOX1 protein, thereby enhancing the drying or salt stress resistance of the plant.

In addition, the CaLOX1 protein of the present invention is preferably composed of the amino acid sequence of SEQ ID NO: 1, and the gene encoding the CaLOX1 protein may preferably be of SEQ ID NO: 2, but is not limited thereto.

In one embodiment of the present invention, an increase in CaLOX1 expression was observed under dry or salt stress conditions (see Example 1), and a transgenic Arabidopsis thaliana overexpressing CaLOX1 was prepared (see Example 2), and abscisic acid , ABA) treatment (see Example 3). In addition, in the transgenic Arabidopsis thaliana overexpressing CaLOX1 , osmotic stress caused by mannitol treatment, salt stress due to NaCl treatment, and resistance to drying stress by dehydration treatment were confirmed (see Examples 4 to 6 ), And by controlling the expression of CaLOX1 , it was confirmed that plants with improved dry or salt stress resistance could be provided.

Accordingly, the present invention provides a transgenic plant in which CaLOX1 (Capsicum lipoxygenase 1) protein is overexpressed to enhance dryness or salt stress resistance.

The plant according to the present invention is a food crop including rice, wheat, barley, corn, soybean, potato, red bean, oats, sorghum; Vegetable crops including Arabidopsis, cabbage, radish, red pepper, strawberry, tomato, watermelon, cucumber, cabbage, melon, squash, onions, onions and carrots; Special crops including ginseng, tobacco, cotton, sesame seed, sugar cane, beet, perilla, peanut, and rapeseed; Fruit trees including apple trees, pears, jujube trees, peaches, sheep grapes, grapes, citrus fruits, persimmons, plums, apricots, and bananas; Roses, gladiolus, gerberas, carnations, chrysanthemums, lilies, tulips; And feed crops including ragras, red clover, orchardgrass, alpha-alpha, tall fescue, perenniallaigrus, and the like, preferably Arabidopsis , but are not limited thereto.

Hereinafter, preferred embodiments of the present invention will be described in order to facilitate understanding of the present invention. However, the following examples are provided only for the purpose of easier understanding of the present invention, and the present invention is not limited by the following examples.

EXAMPLES Example 1: Effect of ABA, salt or dry stress CaLOX1  Induction of gene

To determine whether the CaLOX1 gene is promoted by ABA, salt (NaCl) or drought stress, the expression pattern of CaLOX1 gene was confirmed under the above conditions through qRT-PCR.

After treating ABA (100 μM), drought (dehydrated) and NaCl (200 mM), RNA was extracted from the sampled pepper leaves. Extracted RNA samples were treated with RNA-free DNase to remove genomic DNA and stored at -20 ° C. For reverse transcription PCR, Transcript First Strand cDNA synthesis kit (Roche) was used. At the same time, PCR was performed without reverse transcriptase, and PCR products were used for qRT-PCR to reaffirm genomic DNA contamination in cDNA samples. The synthesized cDNA was amplified using a CFX96 Touch ™ real-time PCR detection system (Bio-Rad, Hercules, CA, USA) together with iQ ^™ SYBR Green Supermix and the specific primers shown in Table 1 below. All reactions were repeated three times. The PCR conditions were as follows: After heating for 5 minutes at 95 ° C, 45 cycles (95 ° C for 20 seconds, 60 ° C for 20 seconds, and 72 ° C for 20 seconds) repeat. qRT-PCR was performed at least twice each in biological and technical replicates. The relative expression level of each gene was calculated by the ΔΔCt method (Livak and Schmittgen 2001), and an internal control gene (18S rRNA) was used for normalization to accurately measure target gene expression.

As shown in FIG. 1, in the ABA stress condition, the expression of CaLOX1 increased after 2 to 4 hours after the ABA treatment, but the significance could not be confirmed. In the dry stress condition, after 6 hours from the dehydration treatment, CaLOX1 Expression was increased and expression was significantly increased after 12 hours. In salt stress condition, CaLOX1 expression was increased from 2 hours after salt treatment, and significant increase was observed after 6 hours.

These results indicate that the expression of CaLOX1 gene is closely related to salt and dry stress.

Example  2. CaLOX1 Production of overexpressed Arabidopsis mutant plants

The CaLOX1 gene was transformed into Arabidopsis thaliana, a model plant, to confirm its function in the plant. A vector was constructed to overexpress the CaLOX1 gene by binding with the CaMV 35S promoter, and 2 lines (# 12, # 16) were selected by transfection into Agrobacterium and finally to Arabidopsis.

Example 3. CaLOX1  Identified reduced sensitivity to ABA stress in overexpressed Arabidopsis mutant plants

No significant increase in expression of CaLOX1 gene was observed by treatment with abscisic acid (ABA) To investigate the role of the CaLOX1 gene, we compared the lengths of roots from pre-germination or germinated ABL- treated roots between CaLOX1 overexpressed transgenic Arabidopsis plants ( CaLOX1- OX # 12, # 16) and wild-type (WT) .

Transgenic and wild-type Arabidopsis plants overexpressing CaLOX1 were grown vertically for 7 days in 0.5X MS containing ABA (0.5 μM, 1.0 μM), and then root lengths were compared. In addition, in order to compare the roots length of the transgenic and wild-type Arabidopsis seedlings overgrown with CaLOX1 by germination after ABA treatment, the plants were grown for 4 days in 0.5X MS and then cultured in the medium containing ABA (0.20 μM) 0,5X MS for 7 days, and the experiment was conducted on 20 seeds and performed independently 3 times.

2, the to as the concentration of ABA is increased, but CaLOX1 the root length of the transgenic and wild-type Arabidopsis seedlings are reduced, transgenic Arabidopsis a CaLOX1 overexpression compared to the wild type of control roots The length decreased slowly. Similar results were also obtained in plants treated with ABA after germination.

The above results indicate that the sensitivity to abscisic acid (ABA) is decreased in the Arabidopsis overexpressing CaLOX1 gene.

Example 4.  CaLOX1  Increased resistance to osmotic stress in overexpressed Arabidopsis mutant plants

To investigate the role of the CaLOX1 gene in response to osmotic stress, we compared the root length, germination rate and green cotyledon percentage between CaLOX1- OX and wild-type controls under osmotic stress conditions.

Transformed and wild type Arabidopsis seedlings overexpressing CaLOX1 were grown vertically for 7 days in 0.5X MS containing mannitol (0. 300, 400, 500 mM), and then root lengths were compared. In addition, transgenic and wild type Arabidopsis thaliana overexpressing CaLOX1 was inoculated and germinated on a plate containing 0.5X MS agar medium containing mannitol (500 mM), and germination rate was measured after 5 days from each of them. And seeded on a plate containing 0.5X MS agar medium containing mannitol (400 mM) in the same manner as in Example 1. After 7 days, the ratio of green cotyledons was confirmed.

As shown in FIG. 3, as the treatment concentration of mannitol increased, the lengths of transgenic and wild-type Arabidopsis seedlings overexpressing CaLOX1 decreased, but the transgenic Arabidopsis thaliana overexpressing CaLOX1 showed a longer root And the ratio of germination and green cotyledons was relatively high.

These results indicate that the tolerance to osmotic stress by mannitol treatment in the Arabidopsis thaliana overexpressing CaLOX1 gene was improved.

Example 5. CaLOX1  Increased resistance to salt stress in overexpressing Arabidopsis mutants

In order to investigate the role of CaLOX1 gene in response to salt stress, the ratio of gonadal germination rate and green cotyledon when germination stress was applied to the pre-germination CaLOX1- OX Arabidopsis and wild-type control were compared. The concentration of chlorophyll and the length of root were compared in the case of salt stress treatment of OX Arabidopsis and wild type control. In addition, the expression patterns of genes related to bilateral yellowing, survival rate, fresh weight, chlorophyll concentration, hydrogen peroxide yield, and salt stress were compared when salt stress treatment was applied to CaLOX1- OX Arabidopsis and wild type control plants Respectively.

Transgenic and wild type Arabidopsis seedlings overexpressing CaLOX1 were seeded on a plate containing 0.5X MS agar medium containing NaCl (200 mM), after which the germination rate and percentage of green cotyledons were determined after 7 days . Also, in the germinated plants, transgenic and wild-type Arabidopsis cells overexpressing 4-day-old CaLOX1 grown on a plate containing 0.5X MS agar medium were transferred to 0.5X MS liquid medium containing NaCl (175 mM), and after 7 days Then, the chlorophyll concentration was measured and transferred to a 0.5X MS liquid medium containing NaCl (100 mM) in the same manner as described above. After 7 days, each root length was confirmed. Transgenic and wild type Arabidopsis plants overexpressing 2-week-old CaLOX1 grown in soil were treated with NaCl (250 mM) and grown for 12 days. Then, the occurrence of yellowing, survival rate, loss of fresh weight and chlorophyll concentration , And Arabidopsis thaliana leaf was stained with DAB solution. After 3 and 6 hours, brown spot was observed to confirm the production of hydrogen peroxide by oxidative stress. Expression patterns of the genes related to salt stress were analyzed by qRT-PCR using the primers shown in Table 2 below.

As shown in Figs. 4 and 5, it was confirmed that the germination rate and green cotyledon ratio were decreased in the plants treated with NaCl before germination, and the transformed Arabidopsis thaliana overexpressing CaLOX1 had a germination rate And green cotyledons decreased slowly. In addition, the reduction of chlorophyll concentration and root length was observed in plants treated with NaCl after germination, and the transgenic Arabidopsis thaliana overexpressing CaLOX1 showed a slow decrease in chlorophyll concentration and root length compared with the wild type control Respectively.

As shown in Figs. 6 and 7, in the case of treatment with NaCl in the vegetative state, it was confirmed that the transgenic Arabidopsis thaliana overexpressing CaLOX1 had a reduced yellowing phenomenon and reduced number of dead plants as compared with the wild type control. In addition, the loss or decrease of the weight and chloroplast was also decreased slowly, and the amount of hydrogen peroxide production was confirmed by confirming the brown spots as a result of DAB staining. In addition, the expression level of the gene, DREB2A, RD20, RD29a, RD29b, P5CS related to salt stress was found to increase substantially as in Arabidopsis thaliana plants over-expressing the CaLOX1 compared to the control group.

These results indicate that the resistance to salt stress by NaCl treatment is enhanced in Arabidopsis thaliana overexpressing CaLOX1 gene.

Example  6. CaLOX1  Overexpressed Arabidopsis mutant plants On dry stress  Identify enhanced resistance to

In order to investigate the role of the CaLOX1 gene in response to dry stress, we compared the survival rate, moisture loss, stomatal aperture, MDA concentration, and dry stress among CaLOX1- OX Arabidopsis and wild type controls in dry stress conditions Expression patterns of the genes were analyzed by qRT-PCR using the primers shown in Table 3 below.

Transgenic and wild type Arabidopsis plants overexpressing 3 weeks of CaLOX1 were dehydrated by stopping watering for 11 days. After that, water was added to the plants again for 2 days so as to recover from dehydration, the survival rate of the plants having rehydrated green leaves was confirmed, and the leaf loss and pore opening degree of the Arabidopsis leaves were checked. In addition, the MDA concentration was measured from the leaves taken 4 and 8 hours after dehydration, and the degree of lipid peroxidation was confirmed. The amount of hydrogen peroxide production by oxidative stress was determined from the leaves collected after 1 and 3 hours of dehydration treatment. Expression patterns of genes related to dry stress were analyzed by qRT-PCR.

As shown in FIGS. 8 to 9, in the dry stress condition, the transgenic Arabidopsis thaliana overexpressing CaLOX1 showed high survival rate, low water loss, and high pore blocking degree as compared with the wild type control, and low lipid peroxidation Oxidative stress was confirmed. In addition, the COR15A gene, DREB2A, RD20, RD29a, expression of RD29b related to drought stress was found to increase substantially as in Arabidopsis thaliana plants over-expressing the CaLOX1 compared to the control group.

The above results indicate that the resistance against the dry stress by the dehydration treatment in the Arabidopsis thaliana overexpressing CaLOX1 gene is improved.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive.

<110> Chung-Ang University Industry-Academy Cooperation Foundation <120> Method for improving resistance to the drought or salt stress          using CaLOX1 in plants <130> Cau20141520KR_PB14-12160 <160> 18 <170> Kopatentin 2.0 <210> 1 <211> 2586 <212> DNA <213> Artificial Sequence <220> <223> CaLOX1 <400> 1 atgttactgg aaaagattgt ggacgtaatc tctgggaaaa atgacgatgg aaaaaagatg 60 aaaggaactg ttgtgttgat gaagaagaat gcattggact ttaatgatgt caatgcttct 120 tttcttgatg gagttcttga gttccttggc aagagagtct ctttgcagtt gatcagctct 180 gttcatggtg atcctgcgaa tggtttacaa gggaaacgta gcaagccagc ttacttggag 240 aactggctca ctacgcgaac cccattagtc gcaggcgaat cagcctttga tgtcacgttt 300 gattgggacg aggatattgg agttccaggg gcatttatca tcaataattt gcacttcaat 360 gagtttttcc ttaaatcact cactcttgaa gacgttccca atcatggcaa gattcatttt 420 gtctgtaatt cttgggttta tcctgctaaa agatacaaat cagaacggat tttctttgct 480 aatcaggcat atcttcccca cgaaactcca gaaccattgc gcgaatacag agaaaaagaa 540 ttagtgacct taagaggaga tggaaatgga aagcttgagg aatgggacag ggtttatgac 600 tatgctttct acaacgactt gggtgatcca gaaagaggcg aagcgtatgc taggactatc 660 ttgggaggat ctgctgagtt cccataccct cggagaggaa gaacaggcag aaagtcaaca 720 aaagcagatc ctaaaagtga aagtaggatt ccattgctta tgagtttaga catctatgta 780 ccaagagatg agcgttttgg acacattaag ttgtcggact tcccgacata tgctttgaaa 840 tccattgttc agttccttat ccccgagttt caggctctct ttgatagcac tcctggtgag 900 tttgacagct ttgaggatgt actgaggctt tatgaaggag gaatcaaatt gccgcaaggc 960 ccttttctca aagccctcac tgacagcatt cctctatcga ttctaaaaga aatcatccga 1020 actgatggtg aagggaaatt caaattccca actcctcaag tcattcaagc ggataaaagt 1080 tcatggagga ctgatgaaga atttgcaaga gaaatgcttg ccggagtaaa tcctgtcata 1140 atcagcagac tccaagagtt ccctccaaaa agcaagctag atactgaagt atatggaaac 1200 caaaacagta caataaccaa agaacatata gagaatgcac tggatgggct aactatcgat 1260 gatgcaatca agacaaacag gctttacata ttaaaccatc atgacatgct tatgccgtat 1320 gtgaggagaa taaacacgac aaacacaaaa ctctacgcct caagaactct gcttttcttg 1380 caagacgatg gaacaatgaa gccaatagca attgaactaa gcttgccaca tccggatgga 1440 gatgaacttg gggctgttag caaagtttat accccagccg atcgagatgt tgagggtacg 1500 atctggcaat tggctaaagc ttatgttgca gtgaatgact cgggtgttca tcagctaatc 1560 agtcactggt tgaatacaca tgcagcaatt gagccgtttg tgattgcaac aaacaggcaa 1620 ctaagcgtgc ttcacccgat tcataaactt ttacatcctc attttcggga cacgatgaac 1680 ataaacgctt tggcaagaca gatcttaatc aatgctggtg gagttcttga gctgacagtt 1740 tttccttcca aatatgcgat ggaaatgtct gctgtagttt acagaaattg ggtcttccct 1800 gaacaagcac ttccggttga tctcgttaag agaggagttg cagtagagga ctcgagttcc 1860 ccacatggcg ttcgcttact aattcaagac tacccatacg ctgttgatgg tttagaaata 1920 tggtcagcaa tcaaaatttg ggtaacagaa tattgcaact tctattataa atcagatgaa 1980 tcagttctga aagatgatga actccaagcc tggtggaaag aagttcggga agaagggcat 2040 ggtgacaaga aagatgaacc ctggtggcct aaaatgcaaa cacgtcaaga gctaatagat 2100 tcttgcacca ttattatttg gatagcatca gcacttcatg cagcagtcaa ttttgggcaa 2160 tacccttatg caggttacct cccaaatcgc ccaacattaa gtcgaagatt catgcctgag 2220 ccaggaactc ctgagtatga agaacttaag acaaatcctg atctggcata cttgaaaaca 2280 atcactcctc aactgcagac attactagga atttctctca tagagatatt gtcaaggcat 2340 acatcagatg aggtttacct tggacagaga gactcatctg aatggacaaa ggaccaagaa 2400 cctcttgctg cttttgagag gtttgggaaa aagttgagtg aaatcgagga tcaaattgta 2460 cagatgaatg gcgatgagaa ttggaaaaat aggtcggggc ctgttaaggt tccatatacg 2520 ttgctctttc ctacaagtga agaaggactc acaggcaaag gaatacccaa cagtgtgtcg 2580 atatag 2586 <210> 2 <211> 861 <212> PRT <213> Artificial Sequence <220> <223> CaLOX1 protein <400> 2 Met Leu Leu Glu Lys Ile Val Asp Val Ile Ser Gly Lys Asn Asp Asp   1 5 10 15 Gly Lys Lys Met Lys Gly Thr Val Val Leu Met Lys Lys Asn Ala Leu              20 25 30 Asp Phe Asn Asp Val Asn Ala Ser Phe Leu Asp Gly Val Leu Glu Phe          35 40 45 Leu Gly Lys Arg Val Ser Leu Gln Leu Ile Ser Ser Val His Gly Asp      50 55 60 Pro Ala Asn Gly Leu Gln Gly Lys Arg Ser Lys Pro Ala Tyr Leu Glu  65 70 75 80 Asn Trp Leu Thr Thr Arg Thr Pro Leu Val Ala Gly Glu Ser Ala Phe                  85 90 95 Asp Val Thr Phe Asp Trp Asp Glu Asp Ile Gly Val Pro Gly Ala Phe             100 105 110 Ile Ile Asn Asn Leu His Phe Asn Glu Phe Phe Leu Lys Ser Leu Thr         115 120 125 Leu Glu Asp Val Pro Asn His Gly Lys Ile His Phe Val Cys Asn Ser     130 135 140 Trp Val Tyr Pro Ala Lys Arg Tyr Lys Ser Glu Arg Ile Phe Phe Ala 145 150 155 160 Asn Gln Ala Tyr Leu Pro His Glu Thr Pro Glu Pro Leu Arg Glu Tyr                 165 170 175 Arg Glu Lys Glu Leu Val Thr Leu Arg Gly Asp Gly Asn Gly Lys Leu             180 185 190 Glu Glu Trp Asp Arg Val Tyr Asp Tyr Ala Phe Tyr Asn Asp Leu Gly         195 200 205 Asp Pro Glu Arg Gly Glu Ala Tyr Ala Arg Thr Ile Leu Gly Gly Ser     210 215 220 Ala Glu Phe Pro Tyr Pro Arg Arg Gly Arg Thr Gly Arg Lys Ser Thr 225 230 235 240 Lys Ala Asp Pro Lys Ser Glu Ser Arg Ile Pro Leu Leu Met Ser Leu                 245 250 255 Asp Ile Tyr Val Pro Arg Asp Glu Arg Phe Gly His Ile Lys Leu Ser             260 265 270 Asp Phe Pro Thr Tyr Ala Leu Lys Ser Ile Val Gln Phe Leu Ile Pro         275 280 285 Glu Phe Gln Ala Leu Phe Asp Ser Thr Pro Gly Glu Phe Asp Ser Phe     290 295 300 Glu Asp Val Leu Arg Leu Tyr Glu Gly Gly Ile Lys Leu Pro Gln Gly 305 310 315 320 Pro Phe Leu Lys Ala Leu Thr Asp Ser Ile Pro Leu Ser Ile Leu Lys                 325 330 335 Glu Ile Ile Arg Thr Asp Gly Glu Gly Lys Phe Lys Phe Pro Thr Pro             340 345 350 Gln Val Ile Gln Ala Asp Lys Ser Ser Trp Arg Thr Asp Glu Glu Phe         355 360 365 Ala Arg Glu Met Leu Ala Gly Val Asn Pro Val Ile Ile Ser Arg Leu     370 375 380 Gln Glu Phe Pro Pro Lys Ser Lys Leu Asp Thr Glu Val Tyr Gly Asn 385 390 395 400 Gln Asn Ser Thr Ile Thr Lys Glu His Ile Glu Asn Ala Leu Asp Gly                 405 410 415 Leu Thr Ile Asp Asp Ala Ile Lys Thr Asn Arg Leu Tyr Ile Leu Asn             420 425 430 His His Asp Met Leu Met Pro Tyr Val Arg Arg Ile Asn Thr Thr Asn         435 440 445 Thr Lys Leu Tyr Ala Ser Arg Thr Leu Leu Phe Leu Gln Asp Asp Gly     450 455 460 Thr Met Lys Pro Ile Ala Ile Glu Leu Ser Leu Pro His Pro Asp Gly 465 470 475 480 Asp Glu Leu Gly Ala Val Ser Lys Val Tyr Thr Pro Ala Asp Arg Asp                 485 490 495 Val Glu Gly Thr Ile Trp Gln Leu Ala Lys Ala Tyr Val Ala Val Asn             500 505 510 Asp Ser Gly Val His Gln Leu Ile Ser His His Trp Leu Asn Thr His Ala         515 520 525 Ala Ile Glu Pro Phe Val Ile Ala Thr Asn Arg Gln Leu Ser Val Leu     530 535 540 His Pro Ile His Lys Leu Leu His Pro His Phe Arg Asp Thr Met Asn 545 550 555 560 Ile Asn Ala Leu Ala Arg Gln Ile Leu Ile Asn Ala Gly Gly Val Leu                 565 570 575 Glu Leu Thr Val Phe Pro Ser Lys Tyr Ala Met Glu Met Ser Ala Val             580 585 590 Val Tyr Arg Asn Trp Val Phe Pro Glu Gln Ala Leu Pro Val Asp Leu         595 600 605 Val Lys Arg Gly Val Ala Val Glu Asp Ser Ser Ser Pro His Gly Val     610 615 620 Arg Leu Leu Ile Gln Asp Tyr Pro Tyr Ala Val Asp Gly Leu Glu Ile 625 630 635 640 Trp Ser Ala Ile Lys Ile Trp Val Thr Glu Tyr Cys Asn Phe Tyr Tyr                 645 650 655 Lys Ser Asp Glu Ser Val Leu Lys Asp Asp Glu Leu Gln Ala Trp Trp             660 665 670 Lys Glu Val Arg Glu Glu Gly His Gly Asp Lys Lys Asp Glu Pro Trp         675 680 685 Trp Pro Lys Met Gln Thr Arg Gln Glu Leu Ile Asp Ser Cys Thr Ile     690 695 700 Ile Ile Trp Ile Ala Ser Ala Leu His Ala Ala Val Asn Phe Gly Gln 705 710 715 720 Tyr Pro Tyr Ala Gly Tyr Leu Pro Asn Arg Pro Thr Leu Ser Arg Arg                 725 730 735 Phe Met Pro Glu Pro Gly Thr Pro Glu Tyr Glu Glu Leu Lys Thr Asn             740 745 750 Pro Asp Leu Ala Tyr Leu Lys Thr Ile Thr Pro Gln Leu Gln Thr Leu         755 760 765 Leu Gly Ile Ser Leu Ile Glu Ile Leu Ser Arg His Thr Ser Asp Glu     770 775 780 Val Tyr Leu Gly Gln Arg Asp Ser Ser Glu Trp Thr Lys Asp Gln Glu 785 790 795 800 Pro Leu Ala Ala Phe Glu Arg Phe Gly Lys Lys Leu Ser Glu Ile Glu                 805 810 815 Asp Gln Ile Val Gln Met Asn Gly Asp Glu Asn Trp Lys Asn Arg Ser             820 825 830 Gly Pro Val Lys Val Pro Tyr Thr Leu Leu Phe Pro Thr Ser Glu Glu         835 840 845 Gly Leu Thr Gly Lys Gly Ile Pro Asn Ser Ser Ser Ile     850 855 860 <210> 3 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> CaLOX1-Forward <400> 3 acgtaatctc tgggaaaaat gacgat 26 <210> 4 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> CaLOX1-Reverse <400> 4 acgtgacatc aaaggctgat tcgc 24 <210> 5 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> 18S rRNA-Forward <400> 5 tatggtgtgc accggtcgtc tcgt 24 <210> 6 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> 18S rRNA-Reverse <400> 6 gcagttgttc gtctttcata aatccaa 27 <210> 7 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> DREB2A-Forward <400> 7 ctacaaagcc tcaactacgg aatac 25 <210> 8 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> DREB2A-Reverse <400> 8 aaactcggat agagaatcaa cagtc 25 <210> 9 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RD20-Forward <400> 9 tggtttccta tctaaagaag ctgtg 25 <210> 10 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RD20-Reverse <400> 10 atacaaatcc ccaaactgaa taaca 25 <210> 11 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> RD29A-Forward <400> 11 cacaatcact tggctccact gttg 24 <210> 12 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RD29A-Reverse <400> 12 acctagtagc tggtatggag gaact 25 <210> 13 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RD29B-Forward <400> 13 gttgaagagt ctccacaatc acttg 25 <210> 14 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> RD29B-Reverse <400> 14 attaacccaa tctctttttc acaca 25 <210> 15 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> P5CS-Forward <400> 15 gaaggattac ttacaacgag atgga 25 <210> 16 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> P5CS-Reverse <400> 16 ctctcctcaa gtctcaacca aatac 25 <210> 17 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> COR15A-Forward <400> 17 gatacattgg gtaaagaagc tgaga 25 <210> 18 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> COR15A-Reverse <400> 18 acatgaagag agaggatatg gatca 25

Claims (5)

A method of promoting dry or salt stress resistance of a plant comprising the steps of:
(a) transforming a gene encoding a CaLOX1 (Capsicum Lipoxygenase 1) protein into a plant; And
(b) overexpressing the CaLOX1 protein in the transformed plant.
2. The method of claim 1, wherein the CaLOX1 protein comprises the amino acid sequence of SEQ ID NO: 1.
2. The method according to claim 1, wherein the gene encoding the CaLOX1 protein comprises the nucleotide sequence of SEQ ID NO: 2.
A transgenic plant having improved dry or salt stress resistance by the method of claim 1.
The method of claim 4, wherein the transgenic plant is a plant which is characterized in that the Arabidopsis thaliana (Arabidopsis).

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