KR20160011174A - Food composition comprising cynanchum wilfordii potentiating testosterone excretion as an active ingredient - Google Patents

Abstract

The present invention relates to a food composition comprising a Cynanchum wilfordii Hemsley extract as an active ingredient for enhancing testosterone secretion. A Cynanchum wilfordii Hemsley extract of the present invention can be used in a food composition for preventing and treating diseases caused by a decrease in testosterone levels, since the Cynanchum wilfordii Hemsley extract increases Testosterone content in leydig testicular cells and in blood of rats.

Description

TECHNICAL FIELD [0001] The present invention relates to a health food composition comprising a white water extract having a testosterone secretion enhancing effect,

The present invention relates to a health food composition comprising a white water extract having a testosterone secretagogue enhancing effect. More particularly, the present invention relates to a health food composition for preventing or ameliorating a disease caused by reduction of testosterone in a menopausal male who can be safely used without toxicity and side effects by using an extract as a natural raw material ( Cynanchum wilfordii Hemsley) .

Sasao has roots that reach into the ground, forming round root roots. The narrow stem has no hairs in the whole, and the leaves are staggered. The leaves have petiole, the edge is flat, the edge is flat. From August to September, the white flower at the end of the branch runs on each other. There are no petals, and the calyx is divided into 5 pieces. There are eight stamens and three styles. The achene fruit has wings and is wrapped in calyx. It is a perennial herbaceous perennial native to China, native to China. It is possible to cultivate everywhere in the country because it is wild, cold tolerant, insecticidal, resistant to weather, etc.

There are two kinds of red and white seaweeds. White cedar is white cedar ( Cynanchum wilfordii Hemsley), which is white cedar (Asclepiadaceae). They are also called other names such as mockery, dwarf grass, sunshine, new mockery, multipurpose roots and have the efficacy of nourishment, blood, blood, It is used for early white hair (early white hair), nervous breakdown, and chronic air bloom.

Polygonum multiflorum has been used for the treatment of liver diseases such as interpolation, xyphosis, hemorrhage, giant wind, Ningxian God, (髓 精 髓), 정수 (((髓 鬚 痰), 정수 鬚 鬚, 悅 鬚), Long striae), a yearly fever

Polygonummultiflorum Thunberg is a dried perennial herbaceous perennial herbaceous plant belonging to the family Porphyra spp., 3-4 years old. In Korea and North Korea, it is traditionally defined that the phytochemicals of sewage are classified into the red sea water and the white sea water. In the Korean Pharmacopoeia, the Korean herbal medicine standard set the enemy seaweed to the seaweed, the white seaweed to the white seaweed (white warriors, Cynanchum wilfordii Hemsley), and the Korea Food and Drug Administration (KFDA) distinguishes Hwaseo and Hwaseo (see Table 1).

On the other hand, menopausal period generally means that when the mature stage of the female reaches the terminal period, the function of the ovary gradually decreases and the ovulation function becomes irregular, the formation of the progesterone is not complete and the reproductive organs decline, Is called a menopause. Most women reach the menopause around the age of 45. During this period, symptoms such as heart palpitations, excitement, anxiety, and irritability develop, and further progression results in complete abolition of the menstrual cycle, which lasts for one to three years. It is necessary to supplement female hormone therapy.

Female hormone is the estrogen and progesterone, which are follicular hormones secreted from the ovaries. Generally, female hormones are often used to refer to estrogen. Estrogen is a female hormone that regulates the lifespan of women through menstruation, pregnancy, and menopause. Estrogen levels in the body affect a wide range of tissues and organs, from the brain to the liver and bone, and estrogen is especially needed to maintain flexibility and steady state of the uterus, urinary, breast, skin, and blood vessels.

Thus, conventional menopause has been used to refer to women's menopause, but recently men's menopause problems due to dietary changes have led to the classification of health functional foods to distinguish between female and menopausal menstrual periods. Table 1 shows the status of raw material approval by functional content of the 2013 health functional food classification.

Functionality of Health Functional Food Classification in 2013 number Functional Functional ingredient name Number Classification Contents One Fatigue improvement Help improve fatigue Hong Kyung Chun Extract One 2 Blood glucose control Helps to suppress postprandial increase in blood sugar Mushroom mycelium One 3 Body fat reduction Help reduce body fat (2), lactoferrin (milk refining protein), chitosan (2), and sea mustard extract (Jansetgen) 7 4 Liver health Helps in liver health Bellflower extract, fermented kelp 2 Helps protect liver from alcoholic damage - 0 5 Blood circulation improvement Help improve the haul Cultured natto bacterium One 6 Improve memory Help improve memory - 0 7 Joint / Bone Health Help with joint health Compound extracts such as rosehip powder (2), complex extracts such as Gassiogalli 4 Helps in bone health Mixed Extract One 8 Blood triglyceride improvement Helps improve blood triglyceride - 0 9 Improving exercise performance / endurance Helps to improve athletic ability - 0 Helps to improve endurance - 0 10 Intestinal health Helps in the proliferation of beneficial bacteria in the intestines and inhibition of harmful bacteria - 0 Controlling your immune system helps your intestinal health - 0 Help with bowel activity smoothly - 0 11 Menopausal Women's Health Help with the health of menopausal women - 0 12 Eye Health Helps improve eye fatigue - 0 Helps in eye health Blueberry fruit extract, Lutein giacantine complex extract 2 13 Skin health Helps maintain skin health from skin damage caused by ultraviolet rays Phosphatidylserine (2), low molecular collagen peptide, finger root extract powder (panduratin), 4 Helps skin moisturize Hyaluronic acid, sodium hyaluronate 2 14 Urination function Helps improve urination by bladder - 0 15 detente Helps relieve tension caused by stress Hong Kyung Chun Extract One 16 Urinary tract health Helps prevent urinary tract infection by inhibiting the absorption of harmful bacteria in the urinary tract - 0 17 Calcium absorption help Helps to absorb calcium - 0 18 Anti-oxidant Helps in antioxidation Ubiquinol One 19 Digestive function Helps improve digestion - 0 20 Blood pressure control Helps reduce high blood pressure  Grape seed enzyme decomposition extraction powder One 21 Prostate Health Helps maintain prostate health - 0 22 Cognitive ability Help improve cognitive abilities Fermented L.helveticus, Platycodon sp. Extract (DRJ-AD) 2 23 Immune function Helps boost immunity Yeast Beta Glucan, Synthetic PLAG, Cordyceps sinensis Extract 3 Helps relieve irritable immune response - 0 24 Decay of cavities Helps reduce the risk of cavities - 0 25 Cholesterol improvement Helps improve blood cholesterol Green tea extract One 26 Irritable skin condition
Improving May help improve skin condition by immunosensitive response Lactobacillus (L. plantarum CJLP133), L.sakei Probio65 2 27 Menopause Men's Health Can help the health of menopausal men MR -10 Compound extracts such as dandelion One sum 37

Testosterone is an interstitial cell of testis, a steroid compound that is produced by cholesterol under the control of the gonadal hormone in the lower body. It has an effect of accumulating nitrogen in the body because it has the expression of secondary sex of male, promotion of spermatogenesis, development of testis, prostate, seminal vesicle and also assimilating protein.

Accordingly, the present invention provides a method for preventing or reducing diseases caused by reduction of testosterone in a menopausal male who can be safely used without toxicity and side effects by using an extract of Cynanchum wilfordii Hemsley, a natural raw material, A health functional food composition having a testosterone secretagogue enhancing effect for improvement.

Korean Patent Publication No. 10-1113593 relates to a pharmaceutical composition for the prevention and treatment of erectile dysfunction. In a hyperlipidemic white paper derived from a high fat diet, red ginseng, which improves erectile function by increasing pressure inside the corpus cavernosum, And an extract obtained by mixing red ginseng, ginseng, and seaweed at a weight ratio of 1: 1: 1 for the prevention and treatment of an erectile dysfunction disease containing an extract of Sasa ginseng as an active ingredient. Korean Patent Publication No. 10-1295125 discloses a method for producing a ginseng extract comprising Schizandra chinensis Baill. Ginseng, Panax ginseng CA Meyer, Polygonum multiflorum Thunberg, and Rubus chingii Hu. As an antioxidant-functional ingredient. However, Polygonum multiflorum Thunberg used in the above composition means an amount of sodium hypochlorite, which is different from white sewage used as a material of the present invention.

The present invention provides a functional food composition having a male hormone testosterone secretagogue enhancing effect which can be safely used without toxicity and side effects by using Baekgoo extract of natural Korean resource.

In order to accomplish the above object, the present invention provides a functional food composition having a testosterone secretion enhancing function comprising an extract of Bacillus subtilis as an active ingredient.

The method of extracting a functional food composition having a testosterone secretagogue enhancing function is characterized by comprising washing water with distilled water and then mixing it with distilled water to extract at least one selected from the group consisting of hot water extraction or an alcohol having 1 to 5 carbon atoms or a mixed solvent thereof as an extraction solvent .

The extract containing 0.01 to 99.9% by weight of the extract as an active ingredient, which is soluble in any one of water, methanol, ethanol, propanol, isopropanol, butanol or a mixed solvent thereof, Compositions and functional foods are provided.

The daily dose of the functional food prepared using the extract as a composition is orally administered in an amount of 10 to 2000 mg / kg per kg of body weight. The testosterone-lowering diseases of the present invention include testosterone hypoperfusion, erectile dysfunction, A functional food composition or a pharmaceutical composition for preventing or ameliorating at least one male menopausal disease selected from the group consisting of decreased urination, decreased sperm motility, decreased sexual desire, decreased physical strength, and increased visceral fat.

The functional food composition may be prepared in one or more forms selected from tablets and capsules, soft capsules, granules, liquids or beverages.

The white water extract having the testosterone secretion enhancing function of the present invention is useful as a functional food composition useful for prevention and improvement of diseases due to reduction of testosterone by increasing testosterone content in Leydig TM3 testicular cells and mouse blood Can be used.

FIG. 1 shows the results of cytotoxicity of Leyding TM3 cells against Bacillus subtilis extract.
FIG. 2 shows the effect of increasing testosterone in Leyding TM3 cells on Bacillus subtilis extract.
FIG. 3 shows the results of comparing the effect of testosterone increase in Leyding TM3 cells on Bacillus subtilis and Saponin extract.
Fig. 4 shows the effect of increasing testosterone in rats against Bacillus subtilis extract.

Hereinafter, the structure and effects of the present invention will be described in more detail with reference to specific examples and comparative examples in order to facilitate understanding of the present invention.

One. Baewooo Oh  Extract preparation

50 g of dried white water was washed with distilled water, and 500 mL was added with distilled water and ethanol, respectively, and the mixture was heated at 100 ° C. and 60 ° C. for 3 hours for extraction. After filtration using a filter paper (Watts 41), the filtrate was concentrated under reduced pressure and freeze-dried at -50 ° C for 48 hours using a freeze dryer. According to the above-mentioned method, 28.74 g (57.5%) of Baekgoo hot water extract and 12.31 g (24.6%) of Baechuoethanol extract were obtained according to the extraction solvent and used as a sample of the following experimental example.

1.1. White  Polar solvent, Nonpolar solvent  Available Fraction  Produce

After completely dissolving in 1 L of distilled water, 1 liter of hexane was added to the separating funnel to separate the water layer and the hexane layer. This process was repeated three times. Chloroform, ethylacetate and butanol were sequentially added through the same procedure to obtain fractions. Each of the fractions thus obtained was filtered through a vacuum filtration apparatus and concentrated. After freeze-drying, the solvent was completely removed It was used in this experiment.

1.1.1. Hexane-soluble fraction separation

After completely dissolving 28.74 g of the hydrothermal extract of Bacillus subtilis in 1 L of distilled water, the mixture was put into a separatory funnel and 1 L of hexane was added to separate the hexane insoluble layer (water layer) and the hexane soluble layer. The hexane insoluble fraction (water layer) was again subjected to the same process three times to collect hexane insoluble fraction and soluble fraction.

1.1.2. Chloroform-soluble fractionation

The chloroform-insoluble fraction (water layer) was mixed with 1 L of chloroform, and then the chloroform-soluble fraction and the insoluble fraction were separated. The chloroform-insoluble fraction (water layer) was subjected to the same process three times to collect chloroform-insoluble fractions and soluble fractions.

1.1.3. Ethyl acetate soluble fraction separation

To the chloroform insoluble fraction (water layer), 1 L of ethyl acetate was added and mixed. Then, the ethyl acetate soluble fraction and the insoluble fraction were separated. The ethyl acetate insoluble layer (water layer) was subjected to the same process three times to obtain an ethyl acetate insoluble fraction and a soluble fraction Respectively.

1.1.4. Butanol soluble fraction fractionation

The ethanolic insoluble fraction (water layer) was mixed with 1 L of butanol, and the butanol-soluble fraction and the insoluble fraction were separated. The butanol-insoluble fraction was repeated three times to collect the butanol insoluble fraction and the soluble fraction.

The hexane-soluble fraction, the chloroform-soluble fraction, the ethyl acetate-soluble fraction and the butanol-soluble fraction were concentrated under reduced pressure and then lyophilized to obtain fractions.

2. Baewooo Oh  By extract Leydig TM3 cell Cytotoxic effect experiments

Leyding TM3 cells were cultured in DMEM medium supplemented with 10% FBS at 37 ° C and 5% CO ₂ . One Leyding TM3 cells were diluted to 1 × 10 ⁵ cells / mL and then dispensed in a 24 well plate 37 ℃, 5% CO ₂ After incubation for 24 hours in an incubator, the extracts were added at concentrations of 0, 10, 50, 100 and 200 μg / mL for 48 hours. Then, 300 μL of MTT solution was added for 2 hours and then the supernatant was removed DMSO was added at 100 uL per well and absorbance was measured at 570 nm in an ELISA autoreader.

FIG. 1 shows the results of cytotoxicity of Leyding TM3 cells against Bacillus subtilis extract. According to the measurement results, it was confirmed that Bacillus subtilis extract did not show toxicity to Leyding TM3 cells at the concentrations (10, 50, 100, 200 ug / mL) used in the experiment.

3. Baewooo Oh  By extract Leydig TM3 cell Testosterone increase effect test

Leyding TM3 cells were cultured in DMEM medium supplemented with 10% FBS at 37 ° C and 5% CO ₂ . One Leyding TM3 cells were diluted to 1 × 10 ⁵ cells / mL and then dispensed in a 24 well plate 37 ℃, 5% CO ₂ The testosterone ELISA kit (ADI) was prepared by centrifugation at 1200 rpm for 5 min. The supernatant was collected by centrifugation at 1200 rpm for 24 hrs. The extracts were added at 0, 10, 50, 100 and 200 μg / Testosterone content was measured using Enzo Life Sciences, USA, at -901-093, 065, 174.

FIG. 2 shows the effect of increasing testosterone in Leyding TM3 cells on Bacillus subtilis extract. As shown in FIG. 2, the extracts of Bacillus thuringiensis were treated at a concentration of 10, 50, 100 and 200 ug / ml in Leydig TM3 cells at 88.2 pg / ml, 125.3 pg / ml, 178.4 pg / ml, 236.2 pg / ml testosterone content increased.

4. Baisho Owa  Of corn oil extract Leydig TM3 cell Increased testosterone in comparison

Leyding TM3 cells were cultured in DMEM medium supplemented with 10% FBS at 37 ° C and 5% CO ₂ . One Leyding TM3 cells were diluted to 1 × 10 ⁵ cells / mL and then dispensed in a 24 well plate 37 ℃, 5% CO ₂ The testosterone ELISA kit (ADI-901-C) was prepared by centrifugation at 1200 rpm for 5 min. The supernatant was then centrifuged at 1200 rpm for 24 h. 093, 065, 174; Enzo Life Sciences, USA).

FIG. 3 shows experimental results comparing the increase of testosterone in Leyding TM3 cells with the extracts of Leuconostoc sp. As shown in FIG. 3, the testosterone content of Leydig TM3 cells was 100 and 200 ug / ml, respectively. The testosterone content of Leydig TM3 was higher than that of the L. acidophilus extract But also increased the number of times.

5. Baewooo Oh  Due to ingestion of extracts In rats  Testosterone increase effect experiment

Male 10-week-old male mice were divided into two groups, one was a control group and the other was a control group. During the breeding period, mice were given free access to solid feeds and tap water. At the same time of day, the water extracts of Bacillus officinalis and Bacillus oxyethans dissolved in distilled water were administered at a concentration of 200 mg / kg, and the control group was given the same amount of physiological saline for 4 weeks Lt; / RTI >

After the last oral administration, blood was collected from the tail of the mouse, and the serum was separated by centrifugation at 10000 rpm for 10 minutes. Testosterone ELISA kit (ADI-901-093, 065, 174; Enzo Life Sciences, U.S.A.) was used to measure the serum testosterone content.

Fig. 4 shows the effect of increasing testosterone in rats against Bacillus subtilis extract. As shown in FIG. 4, the testosterone content of the control group fed with PBS was 109.4 pg / ml, and the amount of testosterone was 200 mg / kg, 200 mg / kg ), The testosterone contents were 1100 pg / ml and 1200 pg / ml, respectively.

From the above results, it was shown that the white wool water extract and the ethanol extract increased the testosterone content in the mouse blood by 10% and 10.9%, respectively.

6. Baewooo Oh  Extract As a composition  Health Functional Food Manufacturing

The health functional food having a testosterone secretagogue enhancing effect containing Baekgoo extract as an active ingredient may be prepared in the form of tablets and capsules, soft capsules, granules, and liquid preparations such that the composition is contained in an amount of 0.01 to 99.9% by weight, Modifications are possible. The health functional food may be produced in an amount such that the white water extract is administered in an amount of 10 to 2000 mg / kg per kg body weight per day.

The health functional beverage composition of the present invention is not particularly limited to the components other than those containing the above components, and may contain various flavors or natural carbohydrates as additional components such as ordinary beverages.

Examples of the above-mentioned natural carbohydrates are monosaccharides such as glucose, fructose; Disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. In addition to the above, natural flavoring agents (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavorings.

In addition to the above-mentioned components, the components of the present invention can be suitably used as flavorings such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates such as cheese and chocolate, pectic acid and its salts, A colloidal thickening agent, a pH adjusting agent, a stabilizer, a preservative, a glycerin, an alcohol, a carbonating agent used in a carbonated drink, and the like. In addition, the components of the present invention may contain flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks.

These health functional foods are effective in preventing or ameliorating male menopausal symptoms due to decreased testosterone such as hypo-testosterone, erectile dysfunction, decreased sperm motility, loss of sexual desire, decreased tack, decreased physical strength, and increased visceral fat.

The present invention relates to a food composition for enhancing testosterone which contains as an active ingredient an extract of Leuconostoc sp. Using an extract of Leptospirosis extract of the present invention. The extract of Leucocyte extract increases the testosterone content in Leydig testis cells and mouse blood, The present invention not only has an excellent effect for prevention and improvement of diseases due to reduction in testosterone but also has a low possibility of occurrence of side effects or toxicity due to natural substances and thus the present invention is effective for the improvement, There are industrial applicability in safe pharmaceutical compositions or functional food compositions.

Claims (6)

White Shuo (White Sheep, Cynanchum wilfordii Hemsley) extract as an active ingredient. The present invention relates to a health functional food composition for preventing or ameliorating menopausal diseases
The health functional food composition for preventing or ameliorating menopausal symptoms according to claim 1, wherein the white water extract is an extract obtained from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
A health functional food composition for preventing or ameliorating male menopausal disease characterized by containing the health functional food composition of claim 1 or 2 as an active ingredient
4. The health functional food composition according to claim 3, wherein the health functional food is prepared by incorporating white water extract in an amount of 10 to 2000 mg / kg of body weight per day
The health functional food composition according to claim 4, wherein the health functional food is prepared in the form of tablets, capsules, soft capsules, granules, liquid form
Prevention or improvement of at least one male menopausal disease selected from the group consisting of hypothyroidism, erectile dysfunction, sperm motility reduction, loss of sexual desire, diminished vigor, decreased physical strength and visceral fat, which comprises the extract of claim 1 or 2 as an active ingredient Health functional food composition

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