KR101295125B1 - A pharmaceutical with anti-oxidative activity, and a healthful food with the pharmaceutical - Google Patents

Abstract

The present invention is an antioxidant functional composition containing Schisandra chinensis Baill., Ginseng ( Panax ginseng CA Meyer), Shao (何首烏, Polygonum multiflorum Thunberg) and Bokbunja ( Rubus chingii Hu) as an active ingredient It is about.
The present invention contains Schisandra chinensis Baill. (Omija), Ginseng (人蔘, Panax ginseng CA Meyer), Shou (何首烏, Polygonum multiflorum Thunberg) and Bokbunja (覆盆子Rubus chingii Hu) as an active ingredient,生地黄, Rehmannia glutinosa (Gaertner) Liboschitz)) relates to an antioxidant functional composition and food formulated as a solid by adding juice and honey.

Description

Antioxidant functional composition and functional food containing same {A pharmaceutical with anti-oxidative activity, and a healthful food with the pharmaceutical}

The present invention is an antioxidant functional composition containing Schisandra chinensis Baill., Ginseng ( Panax ginseng CA Meyer), Shao (何首烏, Polygonum multiflorum Thunberg) and Bokbunja ( Rubus chingii Hu) as an active ingredient It is about.

The present invention contains Schisandra chinensis Baill. (Omija), Ginseng (人蔘, Panax ginseng CA Meyer), Shou (何首烏, Polygonum multiflorum Thunberg) and Bokbunja (覆盆子Rubus chingii Hu) as an active ingredient,生地黄, Rehmannia glutinosa (Gaertner) Liboschitz)) relates to an antioxidant functional composition and food formulated as a solid by adding juice and honey.

Oxidative stress due to free radicals and free radicals has been known to cause denaturation and destruction of lipids, proteins, DNA, enzymes and the like, thereby causing aging and various diseases.

In other words, if the bioprotective ability of antioxidants that can withstand oxidative stress or exposure to excessive free radicals and free radicals, the balance is broken and irreversible destruction of the substrate, protein, DNA, etc. As a result, aging and cancer Various diseases such as complex arteriosclerosis, arthritis and Parkinson's disease are caused.

Here, the action of antioxidants can be divided into enzymatic and non-enzymatic systems, including antioxidant enzymes such as superoxide disputase (SOD), catalase, and peroxidase, and vitamin C, E, and glutathione. Non-enzymatic substances such as).

Meanwhile, in order to prevent and improve diseases caused by oxidative stress, researches on developing antioxidants, inducing the expression of antioxidant enzymes, or developing pharmaceuticals and functional foods containing non-enzymatic antioxidants are in progress. Has been.

Synthetic antioxidants developed so far include butylated hydroxyl anisole (BHA), butylated hydroxyl toluene (BHT) and nordihydro-guaiaretic acid (NDGA), and natural antioxidants are tocopherol, gossypol and sesamol. (sesamol), lecithin (lecitthin), cephalin (cephalin), ether extracts of red pepper, wheat germ, soybean, pepper, extracts from tomatoes, mainly extracts of the flavonoid pigments.

However, synthetic antioxidants are toxic in vivo and may cause allergies and tumors, and also have weaknesses such as being easily destroyed when heat is applied once due to weak temperature.

In addition, natural antioxidants are known to have the advantages of being safe to the living body compared to synthetic antioxidants, but the effect is weak.

Therefore, it is urgent to develop a homeostatic functional composition using natural herbal medicines that are excellent in antioxidant activity than existing natural antioxidants without any toxicity to the living body.

On the other hand, the present invention as an antioxidant functional composition, Schizandra, ginseng, sewage and bokbunja as the main active ingredient, bar schisandra, ginseng, sewage and bokbunja look at.

In addition, the present invention looks at the case of using the raw juice and honey to prepare a solid (膏劑) as a formulation containing the main active ingredient (劑,), look at the sulfur.

First, the present invention is the symptoms of aging and disease-induced symptoms of the human body caused by oxidative stress (허) (東 醫 寶 鑑 虛勞 門, ○ 虛 者 皮毛 肌肉 筋脈 骨髓 氣血 津液 不 足 是 也 [綱目, huh (虛), hair, tendon, 힘 tendon, bone marrow (혈), blood donation (진), etc. are lacking [gangmok]. It can be said that it corresponds to), and he noticed that it was necessary to see both the Gi and Yin Yang () 陽) together.

Therefore, the present invention utilizes ginseng (하), sewage (血), Schisandra chinensis (복), bokbunja (yang) as an active ingredient to configure a composition according to the above point of interest.

Schisandra chinensis Baill. Or Schisandra chinensis Baill., Which is a deciduous wood copy belonging to the Schisandra chinensis , is a dried fruit of Schisandra chinensis , S. sphenanthera Rehd . Et Wils. It is harvested after frost and dried in the sun or steamed.

In Chinese medicine, Schisandra chinensis has five flavors, but the sour taste is the main, and its nature is mild, but it is not crude. ) Entering three scenes, converging wastes in the upper part, moaning underneath, and inserting branch, Saengjin, Jihan It is known to have such action.

In particular, recent pharmacological studies have shown that the main pharmacological ingredients of Schisandra chinensis (schizandrin (C23H32O6)) and gomisin A ~ Q, etc. are mainly contained in the seeds.

Looking at the prior art that used Schisandra chinensis for antioxidant effect, Korean Laid-Open Patent Publication No. 10-2009-0097550 describes a cosmetic composition containing sizandrrin extracted from Schisandra chinensis as a main active ingredient for the antioxidant effect and anti-wrinkle effect of the skin. have.

In addition, the paper "Physical Characteristics and Antioxidant Activity of Schizandra chinensis" (Kwak Sook Kim et al., Journal of the Korean Society of Food Science and Nutrition, Vol. It is disclosed that the antioxidant activity was similar to that of BHT without significant difference.

Ginseng is processed from the root of Panax ginseng CA Meyer, a member of the Araliaceae family. White ginseng is dried when stems and leaves are dried in autumn. Dried red ginseng is processed into sugar and dried is called ginseng.

In oriental medicine, it has been used as a representative herbal medicine with the effect of promoting health and preventing disease, such as being slightly warm, sweet and slightly bitter, entering the rain, lungs, and heart to replenish the energy and make a essence.

Ginseng contains about 5.22% of ginsenoside, among which the content of ginsenosides Rb ₁ , Rc and Rg ₁ is relatively high.

In addition, it contains many kinds of amino acids (peptides), vitamins (vitamin) A, B ₁ , B ₂ , C and so on.

Looking at the prior art using the ginseng for the antioxidant effect, Korean Patent Publication No. 10-2009-0112027 discloses to increase the antioxidant activity by extruding the ginseng with the optimal extrusion molding parameters.

In addition, the article “Antioxidant Activity of Ginseng” (Kim Eun-hye et al., Korea Ginseng Society Journal, Vol. 33, No.1, 1 ~ 7, 2009) shows that ginseng oxidative stress along with antioxidant protection against free radical damage It is disclosed that it has an action of suppressing occurrence.

Sewage is a dried root of Polygonum multiflolum Thunb., A perennial herbaceous plant belonging to the Polygonaceae family, which was planted three to four years before planting in spring and in autumn. After the leaves are dry, cut them into two large pieces and dry them in the sun or on the fire.

Here, sewage means red sewage (赤 何首烏), which is defined in the Korean Pharmacopoeia Herbal Medicine Standards Collection, which is divided into sewage as sewage and white sewage as white sewage.

For reference, in South Korea and North Korea, sewage (類) herbs are traditionally defined as dripping sewage and white sewage, whereas in China and Japan, there is no separate provision for white sewage. (Reference: “Study on the Origins and Names of Sewage and White Sewage” (Cho Hwan-soo et al., Korea Institute of Oriental Medicine 9/1 (Kwon, No. 10), 2003)

In Chinese medicine, the taste is bitter, sweet and sweet, and the nature is warm, so it is said that the ear, the heart, the nerve, the ear, and the interpolation, the icy, It is mainly used for the effect of yangtyeol (혈,), gungpung (祛風), etc., and enters the otoscope of the liver, kidney (주로) and Shinjeong (정 精) and liver blood (肝血) ))

Looking at the prior art using sewage for the antioxidant effect, Korean Unexamined Patent Publication No. 10-2006-0098283 discloses sewage as one of about 10 substances contained in the antioxidant nutrient supplement composition for smokers.

In addition, the article “Antioxidant and Nitrite Scavenging Activity of Plant Extracts” (Korean Society of Food Science and Technology, Vol. 33, No.5, 626 ~ 632, 2001) shows that electron donating ability of DPPH of sewage extracts was over 50%. It is shown that this appeared.

Bokbunja is dried fruit of Rubus chingii Hu, a deciduous shrub belonging to the Rosaceae family, and when the fruit turns from green to greenish yellow in early summer. Take out the fruit and remove it and boil it in boiling water for 2 ~ 4 minutes and dry it in the sun.

In Oriental medicine, the taste is sweet and sour and the nature is warm, and enters the kidneys and bystanders. The sweetness is complementary, the acidity is converging, the sex is warm, but the heat is low. (燥熱) does not have the effect of Xinyang (腎陽) is effective to treat the symptoms of hypoxia caused by the new license (藥 焦 不 固) is a medicine (良藥).

 Bokbunja contains ingredients such as total sugar (17.3%), reducing sugar (8.6%), crude protein (10.6%), crude ash (4.5%), crude fat (3.1%), and crude fiber (3.9%). 11 kinds of alcohols, acids including valeric acid, 20 kinds of carbonyls including hexanal, 5 kinds of hydrocarbons including 2-heptanone, and methyl palmitate Contains fragrance components of three kinds of esters.

It also contains a large amount of polyphenol, which prevents cholesterol lowering, inhibition of hypertension or atherosclerosis, prevention of lipid peroxidation, prevention of aging, lowering of lipid concentration in serum, inhibition of the generation of neutrophil, It has been reported that there is an effect of increasing the resistance of blood vessels.

Looking at the prior art using the bokbunja for the antioxidant effect, Korean Patent Publication No. 10-2004-0006823 discloses the use of bokbunja alcohol extraction extract as an ingredient of the antioxidant functional health food.

In addition, the article “Identification and Activity of Antioxidants in Bokbunja” (Yoonin et al. 6, Journal of the Korean Food Science and Technology, Vol. 34, No.5, 898 ~ 904, 2002) showed that the solubility of EtOAc in bokbunja extracts was the result of DPPH experiment. It is described that strong activity appeared in the fractions.

It is the root of Rehmannia glutinosa (Gaertner) Liboschitz, a perennial herb that belongs to Scrophulariaceae.

In oriental medicine, the taste is sweet and bitter, and the nature is cold, and the heart, the liver, and the god are deaf. Entering (腎 經) to moan and moisturize, and the cold temper enters the heart and liver blood (heart, 血 分), cheongnyang blood By aging heat (설) to the snow (泄熱).

The saccharides contained in dike include D-glucose, D-galactose, D-fructose, sucrose, raffinose and starchose. stachyose, manninotriose and verbascose, accounting for 64.9% of the total.

Looking at the prior art that used the live sulfur in the antioxidant effect, the article "Antioxidant effect of the ginseng gowanhwan and its constituent drug group" (Korean Journal of Herbology, Rye Young Sung et al., Volume 18, No. 3, 2003) Although not tested, it has been shown that the results of experiments with mixed samples of raw sulfur and other drugs showed antioxidant effects.

In summary, it can be seen that the prior art uses Schisandra chinensis, Ginseng, Sewao and Bokbunja mixed with other herbal medicines. It can be seen that it has some degree of antioxidant function.

However, the prior art does not describe the use of Schisandra chinensis, Ginseng, Sewao and Bokbun as the main active ingredients, and thus, there is no indication of the antioxidant function that can be obtained by using Schisandra chinensis, Ginseng, Sewao and Bokbunja together.

In addition, in the prior art, Schisandra chinensis, ginseng, sewage and bokbunja as the main active ingredients, it is not described at all using the raw juice and honey to prepare a solid (膏劑).

Therefore, from the viewpoint of the present invention and the results of the study, it is expected that synergistic effect of antioxidant function will be expected when ingested Schisandra chinensis, ginseng, sewage and bokbunja in combination.

In addition, the formation of koji (ginseng) juice and honey in Schisandra chinensis, ginseng, sewage and bokbunja (膏劑) is expected to maintain the antioxidant effect as well as aesthetic sense.

Accordingly, the present invention was carried out for the antioxidant activity and component analysis experiments for the composition formed with Schisandra chinensis, ginseng, sewage and bokbunja as the main active ingredients, additionally containing fresh juice and honey.

It is an object of the present invention to provide a homeostatic functional composition having no antioxidant effect and having an excellent antioxidant effect than existing natural antioxidants.

An object of the present invention is to provide an antioxidant functional composition containing Schizandra chinensis, Ginseng, Sewage and Bokbunja as active ingredients.

It is an object of the present invention to provide an antioxidant functional composition and food containing Schizandra chinensis, Ginseng, Sewage and Bokbunja as active ingredients, and formulated as a solid by adding fresh juice and honey.

The present invention aims to solve the technical problem by providing an antioxidant functional composition containing Schisandra chinensis, ginseng, sewage and bokbunja as active ingredients.

The present invention provides a prophylactic and therapeutic agent for skin aging formulated in a pharmaceutical unit dosage form by adding a pharmaceutically acceptable carrier, excipient or diluent to an active ingredient including Schizandra chinensis, Ginseng, Sewage and Bokbunja. To solve technical problems.

Examples of the carrier, excipient and diluent include tosse, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Vaginal cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

The pharmaceutical dosage forms may also be used in the form of pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set.

In addition, when formulating an active ingredient including the Schizandra chinensis, ginseng, sewage and bokbunja can be prepared using a conventional diluent or excipient such as fillers, extenders, binders, wetting agents, disintegrating agents, surfactants.

In addition, each of the pharmaceutical dosage forms may be formulated in the form of powders, granules, tablets, capsules, oral preparations such as suspensions, emulsions, syrups, aerosols, suppositories, and sterilized injection solutions according to conventional methods .

In the solid preparation for oral administration, at least one excipient such as starch may be prepared by mixing calcium carbonate, sucrose, lactose, gelatin or the like in the extract. In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used.

The preparation for parenteral administration may include a sterilized aqueous solution, a non-aqueous solvent, a suspension, an emulsion, a freeze-dried preparation, and a suppository.

Examples of the non-aqueous solvent and the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like.

Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The preferred dosage of the active ingredient according to the present invention may be appropriately selected by those skilled in the art depending on the condition and the weight of the patient, the degree of disease, age, sex, drug form, administration route and period.

The extract of the present invention can be administered to mammals such as rats, mice, livestock, humans and the like in various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, muscular, subcutaneous injection.

The present invention is to solve the technical problem by providing an antioxidant functional composition and food formulated as a solid containing Schisandra chinensis, ginseng, sewage and bokbunja as an active ingredient, but added to the saengjihwang juice and honey.

The present invention aims to solve the technical problem by providing an antioxidant functional food by adding a food supplement additive to a component containing Schisandra chinensis, ginseng, sewage and bokbunja or the above formulated ingredients.

Examples of the food to which the above components can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

It may also be added to foods or beverages for the purpose of antioxidant function.

At this time, the amount of the ingredient in the food or beverage may be 0.01 to 20% by weight of the total food weight, the health beverage composition may be added in a ratio of 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml have.

The health functional beverage composition of the present invention is not particularly limited to the other components containing the above components, and may contain various flavors or natural carbohydrates, etc. as additional components, as in general beverages.

Examples of the above-mentioned natural carbohydrates are monosaccharides such as glucose, fructose; Disaccharides such as maltose, sucrose and the like and polysaccharides such as dextrins, cyclodextrins and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. In addition to the above, natural flavoring agents (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavorings.

The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the composition of the present invention.

In addition to the above, the ingredients of the present invention include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, organic acids, and protection. It may further contain a sex colloid thickener, a pH adjuster, a stabilizer, a preservative, a glycerin, an alcohol, a carbonation agent used in a carbonated beverage, and the like.

In addition, the components of the present invention may contain natural fruit juices and pulp for the production of fruit juice beverages and vegetable beverages.

These components can be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

Compositions containing Schisandra chinensis, ginseng, sewage and bokbunja as the active ingredients of the present invention, as shown in the ABST experiment and DPPH experiment, has a significant antioxidant activity effect.

The composition containing Schisandra chinensis, ginseng, sewage, and bokbunja of the present invention, and formed of solid extract from raw sap juice and honey, has a significant antioxidant activity effect as revealed by ABST experiment and DPPH experiment.

The composition containing the present invention Schisandra chinensis, ginseng, sewage, and bokbunja, and the composition formed by the solid juice with raw sulfur juice and honey, as shown in the HPLC experiment, the main pharmacological components for the antioxidant function is maintained, the antioxidant activity effect is remarkable Do.

In the composition formed from the above-mentioned solids of the present invention, the Schizandra chinensis and Bokbunja are treated mainly, and when the liquid components (Jangjihwang juice and honey) are more than twice the powder components (Schisandra chinensis, ginseng, sewage and Bokbunja) This further improving effect is remarkable.

In the composition formed from the above-mentioned solid (인) of the present invention, when the Schisandra chinensis, ginseng, sewage and bokbunja in the same dose, the liquid component is twice the powder component and the antioxidant activity is further enhanced when the fresh green juice and honey are the same weight The effect is remarkable.

The composition containing the present invention Schisandra chinensis, ginseng, sewage and bokbunja as an active ingredient, by taking continuously without bringing side effects, the effect of improving the antioxidant activity is remarkable.

Figure 1a is a table showing the results of performing ABST antioxidant efficacy test in a functional composition according to an embodiment of the present invention.
Figure 1b is a graph showing the results of performing the ABST antioxidant efficacy test in the functional composition according to the embodiment of the present invention.
Figure 2a is a table showing the results of performing DPPH antioxidant efficacy test in a functional composition according to an embodiment of the present invention.
Figure 2b is a graph showing the results of the DPPH antioxidant efficacy test performed on the functional composition according to the embodiment of the present invention.
Figure 3 is a graph showing the results of analyzing the components of the functional composition according to an embodiment of the present invention by HPLC.

The terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary terms and the inventor may properly define the concept of the term in order to best describe its invention It should be construed as meaning and concept consistent with the technical idea of the present invention.

Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention. Therefore, various equivalents It should be understood that water and variations may be present.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Example 1. Koji, including Schisandra chinensis, Ginseng, Sewao and Bokbunja  Produce

1-1. Material processing

First, after removing the foreign substances such as soil, which is attached to the medicine, by hand, secondly, Schisandra chinensis, Ginseng, Sewao and Bokbunja are formed by using a grinder to form a powder. (About 1 hour) Remove moisture.

Here, in the case of Schisandra chinensis constituents are mainly contained in the seed bar to form a powder using the whole Schisandra chinensis.

In addition, in the case of Schizandra chinensis and Bokbunja, it is also possible to form a powder by using a preliminary step (酒蒸) step in advance.

The third processing step is to prevent clumping when forming solids, and the Schisandra chinensis, ginseng, sewage, and bokbunja powder are again filtered through a fine sieve, and the raw turmeric juice is filtered through burlap.

1-2. Material mixing

Mix Schisandra chinensis, Ginseng, Sewao and Bokbunja powder with raw distilled juice and honey, but mix so that dough dough is not aggregated and even.

Here, the mixing ratio of Schisandra chinensis, ginseng, sewage and bokbunja is treated at the same dose, and the amount of raw sap juice and honey can be adjusted to correct a certain sour taste unique to Schisandra chinensis.

A mixing vessel was used as a jar and mixed with a wooden spatula.

1-3. Water bath

Prepare the base of the jar in the bath container (made of iron) (wood chips to keep the bottom of the jar floating in the bath container), seal the jar opening with the jar lid (5 sheets of oiled Korean paper) Grabs it.

Continue to soak for 3 days, but continue to replenish with hot water so that the water does not evaporate and the position of the surface does not fall below the height of the mixed dough in the jar.

On day 4, turn off the fire, lift the jar out, discard the hot water from the bowl, put the jar in the bowl again, pour cold water and soak for 1 day.

On the second day, complete the second bath for a day.

Reference Example 1. Experiment Preparation

1-1. Sample Preparation

Schisandra Ginseng Sewage Bokbunja Fresh green juice honey Sample 1 50 (note) 50 50 50 (note) 200 200 Sample 2 50 50 50 50 150 200 Sample 3 50 50 50 50 300 200 Sample 4 50 50 50 50 200 200 Sample 5 50 50 50 50 150 300

A viscous liquid component is formed in the same manner as in Preparation Example 1, but the mixing ratio of Schisandra chinensis, Ginseng, Sewage and Bokbun is treated at the same dose, and mixed with powder (Schisandra chinensis, Ginseng, Sewage and Bokbunja). Samples 1 to 5 were formed by adjusting the amount of (Saengjihwang juice and honey).

Sample 1 is a sample produced by forming Schizandra chinensis and Bokbunja powder.

Diluted water (third) corresponding to 20.0 times (w / v) of the weight of the samples (No. 1 to 5) was added and diluted, and the filtered solution with a syringe filter (nylon, 0.45 μm) was converted to a raw material conversion concentration of 50 mg. / mL was determined and diluted to a final concentration of 5000, 2500, 1250, 625, 312.5 μg / mL experiment.

Reference Example 2. Aesthetic and Digestion Experiment

1-1. Preparation for experiment

For samples 1 ~ 5, 10 students who were attending Woosuk University Oriental Medicine were selected as sensory evaluation agents, and aesthetic and digestive experiments were performed.

Sensory evaluation is a 7-point measurement method (1 point: worst, 2 points: bad. 3 points: a little bad. 4 points: normal. 5 points: a little good. 6 points: good. 7 points: best.) The smell, texture was performed.

1-2. Experiment result

Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 group
number
Degree flavor total 52 total 45 total 52 total 53 total 54 Average 5.2 Average 4.5 Average 5.2 Average 5.3 Average 5.4 Standard
Deviation 0.6 Standard
Deviation 0.5 Standard
Deviation 0.4 Standard
Deviation 0.46 Standard
Deviation 0.49 incense total 55 total 50 total 49 total 51 total 50 Average 5.5 Average 5 Average 4.9 Average 5.1 Average 5 Standard
Deviation 0.679 Standard
Deviation 0.45 Standard
Deviation 0.3 Standard
Deviation 0.3 Standard
Deviation 0.45 article
Direct
feeling total 54 total 46 total 46 total 49 total 45 Average 5.4 Average 4.6 Average 4.6 Average 4.9 Average 4.5 Standard
Deviation 0.49 Standard
Deviation 0.669 Standard
Deviation 0.49 Standard
Deviation 0.54 Standard
Deviation 0.5

Considering the overall preference, sample 1 scored 52 points for taste, 55 points for aroma, and 54 points for texture.

This is considered to be the result of improving digestive disorders by referring to the fact that Schizandra chinensis and Bokbunja contain seeds, which sometimes complains of digestive disorders even if they are made of powder.

In the case of Sample 2, since the ratio of powder is higher than that of other samples, the overall part such as taste and texture is low.

Sample 3 had a too large amount of fresh sulfur juice, which formed thin and also tended to have a stronger flavor and sour taste.

Sample 4 had the same mixing ratio as that of Sample 1, except that Schizandra chinensis and Bokbunja were not treated mainly.

Sample 5 was the sample with the highest amount of liquid components, and the preference was examined in the order after Samples 1 and 4.

Collectively, in the taste and digestion experiments, the formulation of Sample 1 was found to be the best, and the total weight of the liquid component was found to be best suited to twice the total weight of the powder.

Experimental Example 1 ABST Antioxidant Effect Experiment

1-1. ABST reagent

Antioxidant efficacy test using color change was modified. Dissolve ABTS (2,2'-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid)) in 5 mM potassium phosphate buffer (pH 7.4) at 7 mM concentration and add 0.5x 2.45 mM potassium persulfate (K2S2O8) It was added and allowed to stand at room temperature for 12 hours or more to give a dark blue color.

Immediately before each test, 200 μL of potassium phosphate buffer was added to 96 blood tissue culture plates (Microtest 3072, BD Falcon, USA) and absorbance was measured using an absorbance meter (Spectra Max 340, Molecular Devices, USA). When diluted to 1.0 ± 0.02 was used as the ABTS solution.

1-2. Positive control group

The positive control group was diluted with TroloxTM in ethanol to a final concentration of 62.5 μM and the correction solution was potassium phosphate buffer.

1-3. Experimental Method

After adding 20 μL of extract, reference solution and control solution, each concentration of 96 blood tissue culture plate (Microtest 3072, BD Falcon, USA) to each blood, 180 μL of ABTS solution was added to each blood and left at room temperature for 5 minutes. 734 nm absorbance was measured with an absorbance meter (Spectra Max 340, Molecular Devices, USA).

The test was repeated three times, and the absorbance value of the correction liquid was zero. ABTS radical scavenging ability was converted into percentage. The conversion formula is as follows.

Radical scavenging activity (%) = 100-(absorbance value of test group / absorbance value of control group) X 100

1-4. Statistical processing

The significance of the experimental group was determined by one-way ANOVA and independent sample t- test, which was significant when P <0.05 for the control group. .

1-5. Experiment result

Figure 1a is a table showing the results of performing the ABST antioxidant efficacy test to the functional composition according to an embodiment of the present invention, Figure 1b is a diagram showing a graph.

Referring to Figure 1a, 1b, in the case of ABTS radical scavenging ability, all samples showed concentration-dependent efficacy, NO. Strong activity was shown in the order of 4-5-2-3-1.

In particular, at concentrations of 1250 μg / mL, sample 4 was 87.34%, and the efficacy difference was nearly twice that of 47.40% of sample 1.

Here, the activity of the positive control group was 99.87% of the radical scavenging activity.

It is noted that, from Samples 1 to 5, the antioxidant activity was shown to a significant degree, and it can be seen that the antioxidant activity was strengthened when the solid was formed through the bath treatment as a blending ratio of Sample 4, in particular.

In other words, the liquid component (Saengjihwang juice and honey) is twice the powder components (Schisandra chinensis, ginseng, sewage and bokbunja) and the antioxidant activity was enhanced when the saengjihwang juice and honey are the same weight.

Experimental Example 2 DPPH Antioxidant Efficacy Experiment

1-1. DPPH Reagent

Antioxidant efficacy test using color change was modified. DPPH (2,2-diphenyl-1-picrylhydrazyl) dissolved in ethanol at a concentration of 200 μM was used as a DPPH reagent.

1-2. Positive control group

The positive control group was diluted with TroloxTM in ethanol to a final concentration of 62.5 μM and ethanol was used as the calibration solution.

1-3. Experimental Method

After adding 20 μL of each extract, reference solution and control solution to each blood of 96 blood tissue culture plate (Microtest 3072, BD Falcon, USA), 180 μL of DPPH solution was added to each blood and left at room temperature for 30 minutes. 515 nm absorbance was measured with an absorbance meter (Spectra Max 340, Molecular Devices, USA).

The test was repeated three times, and the absorbance value of the correction liquid was zero. DPPH radical scavenging ability was converted in percentage. The conversion formula is as follows.

Radical scavenging activity (%) = 100-absorbance value of the test group / absorbance value of the control group) X 100

1-4. Statistical processing

The significance of the experimental group was determined by one-way ANOVA and independent sample t- test, which was significant when P <0.05 for the control group. .

1-5. Experiment result

Figure 2a is a table showing the results of performing DPPH antioxidant efficacy test on the functional composition according to an embodiment of the present invention, Figure 2b is a graph showing a graph.

Referring to Figures 2a, 2b, the final concentration up to 2500 μg / mL showed a concentration-dependent efficacy, but except for sample 5 was found to decrease the activity at a concentration of 2500 μg / mL or more.

This is judged to affect the absorbance of 515 nm at high concentration as the color of the sample is yellow.

In light of the results of the ABTS test, it is reasonable to conclude that even at high concentrations, the trend of increasing antioxidant activity is maintained.

The difference in efficacy is no. In the order of 4-5-2-3-1, the same as in the ABTS test, especially at concentration 2500 μg / mL, Sample 4 was 77.68%, more than twice the efficacy difference compared to 35.15% of Sample 1.

Herein, the activity of the positive control group was 96.51% of the radical scavenging activity.

It is noteworthy that, as in the ABST experimental example, the antioxidant activity of the samples 1 to 5 was shown to a significant degree, and in particular, the antioxidant activity was enhanced when the solid was formed through the bath treatment as the blending ratio of the sample 4. You can see the point.

Experimental Example 3. Component Analysis Using HPLC

1-1. Preparation for experiment

Qualitative Analysis for Chemical Fingerprinting of Standards Schisandrin and Ginsenosides Rb1, Rb2, Rc, Re, Rf and Rg1 from Sample 4 Using High Performance Liquid Chromatography (HPLC) Was carried out. (Using HPLC as a method established by Jeonju Institute of Biological Materials)

Experimental conditions were HPLC (Agilent 1200 series with MWD (Multi-Wavelength Detector), detector (Detector: UV 203 nm), column (Column: Agilent Zorbax XDB C18 (4.6 × 150 mm, 5 μm pore size)), column Column temp. Is 35 ° C, mobile phase is H2O / Acetonitrile (A / B) as a gradient elution Initiation 3% B, 10 min 18% B, 25 min 22% B, 55 min 30 % B, 75 min 40% B, 80 min 50% B, 85 min 18% B, 90 min 3% B, Flow rate is 0.8 mL / min, Injection is 20 μL.

1-2. Reference material information

The notation of concentrations used in the experiments were all described as free base.

1-2-1. Ginsenoside Rb1

designation Common Name: Ginsenoside Rb1
(3beta, 12beta) -20-[(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl) oxy]-
12-hydroxydammar-24-en-3-yl2-O-beta-D-glucopyranosyl-beta-D-glucopyranoside Molecular formula C ₅₄ H ₉₂ O ₂₃ Molecular Weight 1109.29 CAS No. 41753-43-9 Source Wako Storage condition cold storage

1-2-2. Ginsenoside Rg1

designation Common Name: Ginsenoside Rg1
(3β, 6α, 12β) -3,12-Dihydroxydammar-24-ene-6,20-diyl bis-β-D-glucopyranoside Molecular formula C ₄₂ H ₇₂ O ₁₄ Molecular Weight 801.01 CAS No. 24427-39-0 Source Wako Storage condition cold storage

1-2-3. Ginsenoside re

designation Common Name: Ginsenoside Re Molecular formula C ₄₈ H ₈₂ O ₁₈ Molecular Weight 947.14 CAS No. 52286-59-6 Source Sigma Storage condition cold storage

1-2-4. Ginsenoside Rf

designation Common Name: Ginsenoside Rf
(3β, 6α, 12β) -3,12,20-Trihydroxydammar-24-en-6-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside Molecular formula C ₄₂ H ₇₂ O ₁₄ Molecular Weight 801.01 CAS No. 52286-58-5 Source Fluka Storage condition cold storage

1-2-5. Ginsenoside Rb2

designation Common Name: Ginsenoside Rb2
20-((6-O-alpha-L-Arabinopyranosyl-beta-D-glucopyranosyl) oxy) -12beta-
hydroxydammar-24-en-3beta-yl2-O-beta-D-glucopyranosyl- beta-D-glucopyranoside Molecular formula C ₅₃ H ₉₀ O ₂₂ Molecular Weight 1079.27 CAS No. 11021-13-9 Source Korea Ginseng Corporation Storage condition cold storage

1-2-6. Ginsenoside Rc

designation Common Name: Ginsenoside Rc
20-[(6-O-alpha-L-Arabinofuranosyl-beta-D-glucopyranosyl) oxy] -12b-
hydroxydammar-24-en-3b-yl2-O-beta-D-glucopyranosyl- beta-D-glucopyranoside Molecular formula C ₅₃ H ₉₀ O ₂₂ Molecular Weight 1079.27 CAS No. 11021-14-0 Source Korea Ginseng Corporation Storage condition cold storage

1-2-7. Schisandrin

designation Common Name: Schisandrin
Schisandrin (7CI) Molecular formula C24H32O7 Molecular Weight 432.56 CAS No. 7432-28-2 Source Korea Food and Drug Administration Storage condition cold storage

1-3. Sample preparation

Take 4 weight 1.2753 g of sample 4 and 80% methanol aq. After addition of 10 mL (v / v) and suspension extraction, the samples were centrifuged for 5 min at × 9,700 g, 2-fold diluted, and injected into HPLC by 20 μL.

1-4. reagent

Methanol [CH ₃ OH] HPLC grade J.T. Baker Acetonitrile [CH ₃ CN] HPLC grade J.T. Baker Water [H ₂ O] HPLC grade J.T. Baker

1-5. Experiment result

Figure 3 is a graph showing the results of analyzing the components of the functional composition according to an embodiment of the present invention by HPLC.

For blank samples, the peak retention time of ginsenoside Rg1 in each ginsenoside standard solution is about 23 minutes, and the ginsenoside Rf is about 50 minutes, ginsenoside Rb1 was about 60 minutes, and chisandrin was about 77 minutes.

However, in the case of ginsenosides Re, Rc and Rb2 in the sample, the peak intensity was weak and could not be confirmed.

It is noteworthy that ginsenosides Rb ₁ and Rg included in ginseng and schisandrin, which are the main pharmacological components of Schisandra chinensis, as antioxidant active ingredients in water-treated solids containing Schizandra chinensis and Ginseng. ₁ and Rf were analyzed.

Looking at Reference Example 2, Experimental Example 1, Experimental Example 2 and Experimental Example 3, it can be seen that the composition containing Schisandra chinensis, Ginseng, Sewage and Bokbunja as the main active ingredients has a significant degree of antioxidant activity.

It can also be seen that the aesthetics can be improved when the Schizandra chinensis and Bokbunja are main treated, and the liquid ingredients (Saengjihwang juice and honey) are more than twice the powder components (Schisandra chinensis, Ginseng, Sewao and Bokbunja).

In addition, it is said that antioxidant activity is enhanced when Schisandra chinensis, ginseng, sewage and bokbunja have the same dose, the liquid component is twice the powder component, and the saengjihwang juice and honey are the same weight.

In addition, it can be seen that the main pharmacological components of Schisandra chinensis and ginseng, which can function as antioxidants, were also analyzed in solid products formed through the bath treatment.

Claims (9)

delete delete delete Omija ( Schisandra chinensis Baill.), Ginseng (人蔘, Panax ginseng CA Meyer), Shou (何首烏, Polygonum multiflorum Thunberg) and Bokbunja (覆盆子Rubus chingii Hu) are each formed into powder and contain the same weight.
It is formulated as solid by adding juice and honey of Rehmannia glutinosa (Gaertner) Liboschitz), wherein the weight of the juice and honey is the same weight, and the total weight of the juice and honey is Schisandra chinensis Antioxidant functional composition, characterized in that more than twice the total weight of ginseng, sewage and bokbunja.
delete delete An antioxidant functional food characterized by further adding a food acceptable food additive to the composition according to claim 4.
The method of claim 7,
The Schizandra chinensis and Bokbunja Antioxidant functional foods, characterized in that using the main treatment (酒蒸).
delete

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