KR20160001513U - Continuous cell culture device for maintaining the viability of the mobile - Google Patents

Abstract

The continuous cell culture apparatus for maintaining the viability of movement of the present invention is provided with a culture container having a space capable of selectively determining the closed state, wherein the culture medium is injected into the space, the cell inoculation, And allowing the cell to be cultured under the condition that the space is open. The culture container is provided with an environment for maintaining cell viability by transferring an external temperature upon movement or storage after completion of cell culture.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a continuous cell culture device,

The present invention relates to a continuous cell culture apparatus for maintaining the viability of migration, and more particularly, to a cell culture apparatus capable of injecting, culturing, desorbing, and obtaining cells and culture liquid through a culture vessel, To maintain the viability of cells during the movement of the culture container.

In cell culture, there are single culture (adherent culture) in which cells adhere to an incubator and proliferate, and suspension culture in which cells grow in a floating state.

Most animal cells adhere to the surface and grow, and their growth rate is very slow compared to that of microorganisms. Therefore, productivity is low and they are likely to be contaminated by microorganisms during culture.

However, the conventional methods in the cell surface adhesion culture have difficulties in maintaining the hermeticity, and thus have a problem of being easily contaminated after a certain period of cell acquisition.

In addition, when the culture cells are moved outside the laboratory, separate sealing means must be provided. However, there is no case in which complete sealing is successful.

However, it is difficult to maintain the uniformity of the cultured cells because it is another passage when the container is moved.

In order to solve the above problems, an apparatus for culturing cells in a series of the present invention is a device for selectively and repeatedly injecting, culturing, desorbing, and obtaining cells and a culture solution in a culture vessel, It is possible to obtain cells and to allow the cells remaining in the culture vessel to be repeatedly cultured while preventing contamination and ensuring uniformity of cultured cells.

The object of the present invention is to maintain the viability of the cell by improving the safety when the culture container is moved by continuing conduction of the mammalian or higher temperature to the culture container when the cell container is moved.

That is, the temperature of the mammal or more is not a specially prepared device, but the temperature is transferred to a portion of the body by closely adhering the culture container to the band, fixing the band to the body, or moving the culture container to the temperature generating means, So that the culture container can be easily moved without difficulty.

In addition, the object of the present invention is to provide an incubation apparatus capable of supplying an optimal culture temperature and a gas necessary for cell proliferation to a culture container through a culture environment means which provides an optimal culture environment for cell proliferation while storing a culture container, In order to improve the performance of the system.

The purpose of the present invention is to allow the cells to be desorbed from the bottom of the culture container in a closed state.

In order to accomplish the above object, the present invention provides a continuous cell culture apparatus for maintaining viability of migration, comprising a culture container having a space for selectively determining a closed state, The cell culture can be cultivated in a state where the space is open, and the culture container can be maintained in a state where the cell is viable For example.

According to the present invention, the temperature allows the culture vessel to be brought into close contact with the surface of the body having a temperature equal to or higher than that of the mammals to enable temperature transfer.

According to the present invention, the temperature allows the culture vessel to be brought into close contact with the surface of the portable temperature generating means to enable temperature transfer.

According to the present invention, the temperature allows the incubation vessel to adhere to the surface of the portable electronic device to enable self-heating temperature transition.

According to the present invention, the culture container is provided with a tessellation step on the surface so that it can be fixed to the temperature conductive object through the band.

According to the present invention, in the process of obtaining the cells, cells are obtained except for a part of the whole cultured cells, thereby enabling repeated culturing of the cells remaining in the culture container.

According to the present invention, the whole cultured cells are obtained in the process of obtaining the cells, and then the culturing vessel is allowed to repeatedly perform the culture injection, the cell inoculation, the culture, the desorption, and the obtaining.

According to the present invention, the culture container is provided with a closed passage, and the fluid, gas and cells are carried into and out of the space from the outside, and the culture container is provided with a circulation filter capable of circulating the gas necessary for cell culture in the space do.

According to the present invention, a scraper is installed in the space to scrape the cells by moving the scraper, thereby removing the cells from the bottom of the space.

According to the present invention, the closed passage is provided on the surface of the culture container, and the closed passage is made of a soft block to seal the space, and the fluid, gas and cells can be loaded and unloaded into the space when the needle of the syringe penetrates. At the same time, when the needle is removed, the space is sealed by the elasticity of the sealed passage.

According to the present invention, the gas circulated through the circulation filter into and out of the space allows gas containing at least one of carbon dioxide and oxygen to flow in and out. The circulation filter includes a channel installed at the side of the culture vessel, And a filter provided inside the valve.

According to the present invention, the scraper rotates or moves while contacting the inner surface of the space through a magnetic force to scrape the cells. When the moving member is moved close to the lower surface of the culture container, the scraper and the moving member are moved The cells are cultured in a continuous manner.

According to the present invention, the scraper forms a plurality of blades on the lower surface which is in contact with the bottom surface of the space.

According to the present invention, the circulation filter mounts a clip on a pipeline so that the pipeline can be selectively closed and opened.

In order to solve the above problems, the continuous cell culture apparatus that maintains the viability of migration of the present invention is capable of selectively and repeatedly injecting, culturing, desorbing, and obtaining cells and culture liquid while maintaining the hermeticity of the culture vessel, It is possible to obtain the cells except for a part of the whole cultured cells so that the cells remaining in the culture container can be repeatedly cultured while preventing the contamination of the cultured cells and ensuring uniformity.

The continuous cell culture apparatus that maintains the viability of the present invention maintains the viability of the cell by improving the safety when the culture vessel is moved by continuing conduction of the temperature of the mammal or higher in the culture vessel when the cell culture vessel is moved. .

That is, the temperature of the mammal or more is not a specially prepared device, but the temperature is transferred to a portion of the body by closely adhering the culture container to the band, fixing the band to the body, or moving the culture container to the temperature generating means, So that the culture container can be easily moved without difficulty.

In addition, the continuous cell culture apparatus that maintains the viability of the present invention maintains the optimal culture temperature and the gas necessary for cell proliferation in a culture container by means of a culture environment means that provides an optimal culture environment for cell proliferation while storing the culture vessel Thereby improving the proliferation of the cell culture.

Brief Description of the Drawings Fig. 1 is a perspective view showing a continuous cell culture apparatus that maintains viability of movement of the present invention. Fig.
FIG. 2 is a perspective view showing a state in which a band is attached to a continuous cell culture device that maintains viability of movement of the present invention. FIG.
3 is a longitudinal sectional view showing a state in which a band is attached to a continuous cell culture apparatus that maintains viability of movement of the present invention.
FIG. 4 is a cross-sectional view showing a state in which a band is attached to a continuous cell culture device that maintains viability of movement of the present invention. FIG.
FIGS. 5 to 10 are flowcharts showing steps of culturing cells using a continuous cell culture apparatus that maintains viability of movement of the present invention. FIG.
FIG. 11 is a perspective view showing another embodiment of a continuous cell culture apparatus that maintains viability of movement of the present invention. FIG.
12 is a perspective view showing various embodiments of a scraper among continuous cell culture apparatuses that maintain viability of movement of the present invention;
13 is a perspective view showing a culture environment means for storing a continuous cell culture apparatus that maintains viability of movement of the present invention;
FIG. 14 is a perspective view showing another embodiment of a continuous cell culture apparatus that maintains viability of movement of the present invention. FIG.

Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings.

First, in the drawings, it is noted that the same components or parts are denoted by the same reference numerals as possible. In describing the present invention, a detailed description of related known functions or configurations will be omitted so as not to obscure the gist of the present invention.

FIG. 1 is a perspective view showing a continuous cell culture apparatus for maintaining the viability of movement of the present invention, FIG. 2 is a perspective view showing a state in which a band is attached to a continuous cell culture apparatus for maintaining viability of movement of the present invention, FIG. 4 is a longitudinal sectional view showing a state in which a band is attached to a continuous cell culture device that maintains the viability of movement of the present invention. And FIGS. 5 to 10 are flowcharts showing the steps of culturing cells using a continuous cell culture apparatus that maintains the viability of movement of the present invention. FIG. 11 is a flow chart of a continuous cell culture Fig. 12 is a perspective view showing another embodiment of the apparatus. Fig. 12 is a perspective view showing a variety of scrapers FIG. 13 is a perspective view showing a culture environment means for storing a continuous cell culture apparatus that maintains viability of movement of the present invention, and FIG. 14 is a perspective view of a continuous cell culture apparatus for maintaining viability of movement of the present invention And is a perspective view showing another embodiment.

First, as shown in FIGS. 1 to 10, the continuous cell culture apparatus for maintaining the viability of movement of the present invention is provided with a culture container 100 formed into a polygonal or circular shape.

The culture container 100 is formed with a closed space 101 therein.

The culture container 100 is made of a soft plastic material, and the size of the space 101 can be varied by external pressure or force.

The culture container 100 is provided with a culture container 100 having a space capable of selectively determining an airtight state, in which the culture medium is injected into the space 101, the cells are inoculated, desorbed, and obtained , Allowing the cells to be cultured in the open space.

In addition, in the process of obtaining the cell 11, except for a part of the whole cultured cells 11, the cell 11 is obtained to enable the repeated culturing of the cells 11 remaining in the culture container 100.

In addition, the entire cultured cells are obtained in the process of obtaining the cell 11, and then the culture solution is injected into the culture container 100, the cells are inoculated, the culture is carried out, the desorption is performed repeatedly.

The culture container 100 is equipped with a sealed passage 110 and a circulation filter 120 so that the cells 11 can be cultured continuously.

First, the sealed passage 110 is installed in the culture container 100 to enable the infusion of the culture medium 12 into the space 101, the inoculation of the cells 11 into the culture medium 12, and the process of obtaining the cells 11, and even when the above- State is maintained.

That is, the closed passage 110 is provided on the side surface of the culture container 100 so that fluid, gas and cells 11 can be carried into and out of the space 101 from the outside.

To enable this, the closed passage 110 is provided on the surface of the culture container 100, and the closed passage 110 is formed of a soft block and installed in the space 101.

At this time, a syringe is usually used to inject the culture medium 12 and the cells 11 through the sealed passage 110 and to obtain the cells 11 externally.

In other words, after penetrating the sealed passage 110 through the needle of the syringe, the culture medium 12 filled in the syringe is injected into the space 101, or the cell 11 desorbed after culturing in the culture container 100 is injected into the syringe And sucked out to the outside.

When the needle is taken out from the sealed passage 110 after the injection of the culture liquid 12 or the cell 11 is obtained, the sealing passage 110 itself is sealed by the elasticity of the sealed passage 110, so that the hermeticity of the space 101 can be maintained.

The circulation filter 120 is installed to inject gas necessary for culturing the cells 11 into the space 101 of the culture container 100.

That is, the culture container 100 is stored in the culture environment means 200 at the time of culturing the cells 11, and is supplied with the gas necessary for culturing the cells 11 while being supplied with an appropriate temperature.

At this time, the culture container 100 allows the gas to flow in and out of the space 101 through the circulation filter 120.

13, the gas enters and exits into the space 101 of the culture container 100 by generating a negative pressure inside the culture environment means 200 to change the size of the space 101 of the culture container 100, So that the gas necessary for culturing the cell 11 is allowed into and out of the space 101 inside the means 200.

Here, the gas includes at least one of carbon dioxide and oxygen.

The specific configuration of the circulation filter 120 is as follows.

The circulation filter 120 includes a channel 121 installed on a side surface of the culture vessel 100, a valve 122 installed at an end of the channel 121, and a filter 123 installed inside the valve 122.

A clip 124 is attached to the pipeline 121 so that the pipeline 121 can be selectively closed and opened.

When the culture container 100 is stored in the culture environment means 200, the clip 124 allows the gas to flow into and out of the space 101 by opening the channel 121. When the culture container 100 is to be taken out of the culture environment means 200, The pipe 121 is interrupted to seal the space 101 of the hermetically sealed container.

Since the cells 11 cultivated on the bottom surface of the space 101 of the culture container 100 are not easily detached by the adhesive force, the scraper 130, which is a separate tool for desorbing the cells 11, is installed inside the space 101.

That is, the scraper 130 scatters the cells 11 while rotating or moving inside the space 101 through a mechanical external force, a magnetic force, a potential energy, or the like so that the cells 11 are removed from the bottom surface of the space 101.

In particular, as shown in FIG. 11, the scraper 130 constitutes a rectangular culture container, but there is no limitation on the shape of a culture container such as a circular or triangular polygon having a surface to which cells can adhere.

First, the scraper 130 has a method of removing cells 11 through a mechanical external force as follows.

11, the center shaft 133 is rotatably installed on the bottom surface of the space 101, and the center shaft 133 is exposed to the outside of the culture container 100, and the scraper 130 is interlocked with the rotation of the center shaft 133, The scraper 130 rotating on the bottom surface of the scraper 101 is scratched and scraped off the cell 11.

When the scraper 130 is rotated to remove the cells 11, the culture container 100 is formed into a cylindrical shape, and the scraper 130 rotates about the central axis 133 at the inner periphery of the space 101, thereby removing the cells 11.

In addition, the method of removing cell 11 through magnetic force is as follows.

The moving member 140 provided separately is brought close to the outer lower surface of the culture container 100 and connected to the scraper 130 by a magnetic force so that the scraper 130 is interlocked in the space 101 by the movement of the moving member 140, Scrape off.

That is, the scraper 130 and the moving member 140 may be integrally connected to each other by a magnetic force by providing a metal body 134 or a magnetic body 141.

In the cell 11 removal method using position energy, the culture container 100 is inclined, and the scraper 130 having its own weight slides on the bottom surface of the space 101 to scrape off the cell 11.

The scraper 130 configured as described above is formed with a culture groove 132 at an upper portion thereof to fill the culture liquid 12 and cultivate the cells 11.

That is, in the apparatus for culturing the cells 11 in succession of the present invention, the culture medium 12 is injected into the space 101, or injected into the culture grooves 132 of the space 101 and the scraper 130 to inoculate the culture medium 12 into the culture medium 12.

The lower surface of the scraper 130, which is in contact with the bottom surface of the space 101, forms a plurality of blades 131.

The scraper 130 has a lower surface contacting with a bottom surface of the space 101 to form a plurality of blades 131. The blade 131 is formed to have a corner angle so that the cells 11 are separated from the bottom surface by friction with the bottom surface of the space 101, .

Alternatively, the scraper 130 forms a plurality of blades 131, which are in contact with the bottom surface of the space 101, and the blades 131 are formed so as to have continuous corners of the blades 131 so as to contact or not contact the bottom surface of the space 101.

The scraper may be made of polyethylene, polypropylene (PP), polyamide (PA), polyacetal (POM), polyvinyl chloride (PVC), polyester (PET), polymethylpentene (PC), vinyl acetate (PVAc), polyvinyl alcohol (PVA), polyvinyl alcohols (PVA), polyvinyl alcohols (PVA), polyvinyl alcohols Materials selected from phenol resin (PF), urea resin (UF), melamine resin (MF), epoxy resin (EP), polyurethane (PUR), unsaturated polyester resin (UP) and metal are applied.

One or more culturing vessels 100 constructed as described above are provided and are connected in parallel through the circulating filter 120 in succession.

That is, as shown in FIG. 14, the plurality of culture vessels 100 are sequentially arranged, and the culture vessels 100 arranged adjacent to each other on the basis of the one culture vessel 100 are sequentially connected to the circulation filters 120, So that a plurality of culture vessels 100 can be integrally introduced and removed through the circulation filter 120 of FIG.

A method for continuously culturing cells through the above-described culture apparatus is as follows.

Culturing the cells 11 by inoculating the cells 11 into the inner space 101 of the closed culture vessel 100 and culturing the cells 11 when the density of the cells 11 cultured in the space 101 of the culture vessel 100 exceeds the reference value, Removing the cells 11, and obtaining cells 11 desorbed from the culture vessel 100 maintaining the closed state.

As shown in FIG. 4, the step of culturing the cell 11 comprises the step of injecting the culture medium 12 into the space 101, the step of inoculating the cell 11 into the culture medium 12, and the step of propagating the cell 11 into the culture medium 12 .

In the step of propagating the cell 11 to the culture solution 12, the cell 11 is inoculated into the culture solution 12, and the culture container is stored in the culture environment means 200 to culture the cells.

At this time, the culture environment means 200 has a culture temperature of 0 ° C to -42 ° C, and supplies gas required for cell culture 11 to the culture container.

At the same time, the clip is released from the circulation filter to open the channel.

The culture environment means 200 supplies the gas necessary for culturing the cells 11 to the inside and repeatedly generates negative pressure so that the gas can be repeatedly introduced into the culture container 100.

That is, as shown in FIG. 7, the culture container 100 is made of a soft material, and the size of the space 101 is varied while being compressed by external pressure such as negative pressure.

Then, as the size of the space 101 is resiliently restored to its original state, the gas existing inside the culture environment means 200 is sucked into the space 101 and the gas is taken in and out.

Here, the gas enters and exits the space 101 through the circulation filter 120 installed in the culture vessel 100.

Particularly, the clip 124 is mounted on the circulation filter 120, and the clip 124 selectively presses or releases the circulation filter 120 to determine the open / close state of the circulation filter 120.

After the cell 11 is proliferated in the culture container 100 in the culture environment means 200, the circulation filter 120 is closed through the clip 124 to obtain the cell 11, so that the culture container 100 is switched to the closed state .

Next, as shown in FIG. 9, after the culture container 100 is removed from the culture environment means 200, the moving member 140 having a magnetic action with the scraper 130 is brought into close contact with the lower surface of the culture container 100, The scrapers 130 are interlocked and scrape off the cells 11 attached to the bottom surface of the space 101.

Alternatively, the step of desorbing the cells 11 allows the scraper 130 installed in the space 101 to rotate and move through the mechanical energy to scrape the cells 11 to desorb from the bottom of the space 101.

That is, the scraper 130 is rotatably coupled to the inside of the space 101 through the center shaft 133, and the center shaft 133 is exposed to the outside of the space 101 to rotate the center shaft 133 to interlock the scraper 130, In the same manner as in the first embodiment.

Alternatively, the scraper 130 moves through the potential energy to scrape off the cells 11, thereby leaving them at the bottom of the space 101.

That is, the culture container 100 is inclined, and the scraper 130 having its own weight slides on the bottom surface of the space 101 to scrape off the cells 11.

Thereafter, the syringe needle is infiltrated into the other closed passage 110 except for the closed passage 110 into which the culture liquid 12 is injected, and the cells 11 cultured through the needle through the negative pressure of the syringe are sucked into the syringe.

Here, as shown in FIG. 10, in the step of obtaining the cell 11, a part of the desorbed cells 11 are left, and the remaining cells 11 are allowed to repeatedly grow.

As described above, in the method of culturing the cells 11 in the series of the present invention as described above, the above-described culture medium 12 injection, cell 11 inoculation, cell culture 11, cell 11 release, Thereby enabling stable and repeated cultivation of the post-cell 11.

In addition, as shown in FIGS. 2 to 4, the continuous cell culture apparatus that maintains the viability of the movement of the present invention as described above, locks the circulation filter 120 through the clip when the cell culture is completed, Thereby sealing the space 101 of 100.

Thereafter, in order to move the culture container 100, the temperature of the mammal or higher should be constantly transmitted to the culture container 100 so as to be able to move while maintaining the viability of the cells.

At this time, there is a problem that the culture container 100 is somewhat uncomfortable and inconvenient to install after storing the culture container 100 in a specially provided temperature generating means when it is moved to an aircraft, a ship, an automobile or the like.

In order to compensate for the above-mentioned disadvantages, in the present invention, if the disposing step 300 is provided on the surface of the culture container 100 and fixed to the means for generating a portable temperature through the disposing step 310, But it can maintain cell viability when moving.

Here, the means for generating the temperature may include all means for generating a temperature equal to or higher than the mammal body temperature.

That is, the culture container 100 can be fixed to a part of the body, for example, arms, legs, and torso through a band, and then moved to the transportation means, thereby facilitating the movement of the culture container 100.

Alternatively, it is possible to carry out the above-described effects by moving the culture container 100 in close contact with a temperature generating means which is easy to move and carry, such as a mobile phone or a hand stove, as well as the body.

As a result, when the cell culture is completed, the temperature of the mammal is continuously transferred to the culture container to move the culture container 100, thereby improving the safety when the culture container 100 is moved, thereby maintaining the cell viability.

That is, the temperature of the mammal or more is not a specially provided device, but the culture container 100 is adhered to a part of the body by fixing it with the band 310 and then moved, or the culture container is brought into close contact with the temperature generating means, So that the culture container can be easily moved without difficulty.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the inventions. Will be clear to those who have knowledge of.

10: Culture apparatus 11: Cell
12: Culture medium 100: Culture container
101: space 110:
120: circulation filter 121: duct
122: valve 123: filter
124: clip 130: scraper
131: blade 132: culture groove
133: center shaft 134: metal body
140: moving member 141: magnetic substance
200: culture environment means 300:
310: Band

Claims (14)

A culture container having a space capable of selectively determining a closed state is provided,
It is possible to inject a culture solution, inoculate cells, desorb it, and obtain it into the space while the space is sealed,
The cells can be cultured in a state where the space is open,
Wherein the culture container is provided with an environment for maintaining cell viability by transferring an external temperature upon movement or storage after completion of cell culture.
The method according to claim 1,
Wherein the temperature allows the culture vessel to be brought into close contact with a surface of a body having a temperature higher than that of a mammal to enable temperature transfer.
The method according to claim 1,
Wherein the temperature allows the culture vessel to adhere to the surface of the portable temperature generating means to enable temperature transfer.
The method according to claim 1,
Wherein said temperature is such that the culture container is brought into close contact with the surface of the portable electronic product to enable self-heating temperature transition.
The method according to claim 1,
Wherein the culture container is provided with a tessellation step on its surface so that it can be fixed to the temperature-guiding object through the band.
The method according to claim 1,
Wherein a cell is obtained except for a part of the whole cultured cells during the process of obtaining the cells to enable repeated culturing of the cells remaining in the culture container.
The method according to claim 1,
Wherein the whole cell culture is obtained in the course of obtaining the cells, and then the culture medium is injected into the culture vessel, the cells are inoculated, the culture is carried out, the elimination is carried out repeatedly, and the resultant cells are maintained in viable state.
The method according to claim 1,
Wherein the culture container is provided with a closed passage for introducing and discharging fluids, gases and cells from the outside into the space, and the culture container is provided with a circulation filter capable of circulating the gas necessary for cell culture in the space A continuous cell culture device that maintains viability of migration.
The method according to claim 1,
Wherein a scraper is installed in the space to scrape the cells by the movement of the scraper so as to desorb the cells from the bottom of the space.
9. The method of claim 8,
One or more sealing passages may be provided on the surface of the culture container,
The sealing passage is made of a soft block to seal the space,
Wherein the space is sealed by the elasticity of the closed passage when the needles are removed while enabling fluid, gas, and cells to be introduced into and removed from the space when the needles of the syringe are infiltrated.
9. The method of claim 8,
The gas circulated through the circulation filter into the space is allowed to flow in or out of a gas containing at least one of carbon dioxide and oxygen,
The circulation filter includes a channel installed on a side surface of the culture container,
A valve installed at an end of the conduit,
And a filter provided inside the valve.
10. The method of claim 9,
The scraper rotates or moves while contacting the inner surface of the space through a magnetic force to scrape the cells,
Wherein when the moving member is moved close to the lower surface of the culture container, the scraper and the moving member are interlocked by the magnetic force. Wherein the cell culture apparatus further comprises:
10. The method of claim 9,
Wherein the scraper has a plurality of blades formed on a lower surface thereof which is in contact with a bottom surface of the space.
12. The method of claim 11,
Wherein the circulation filter is provided with a clip on a channel so that the channel can be selectively closed and opened.

Images