KR20150139427A - Hypoglycaemic agent and the preparation for a granule type of hypoglycaemic agent - Google Patents

Abstract

The present invention relates to a hypoglycemic agent and a manufacturing method of a granule thereof, which comprises 15-30% of Astragalus membranaceus, 10-15% of Dioscorea japonica, 10-15% of Pueraria montana, 10-15% of Adenophora triphylla, 8-15% of Atractylodes japonica, 10-15% of Scutellaria barbata, 2-5% of Poria cocos, 5-10% of Momordica charantia L., 5-10% of Polygonatum odoratum, 5-10% of Cornus officinalis, 3-5% of Rehmanniae radix preparata, 2-5% of Zea mays, and 1-5% of Glycyrrhiza uralensis Fischer. More specifically, various types of mixed herbal medicinal compositions are scientifically mixed and configured, thereby maintaining a normal level of blood glucose, and preventing diabetic complications.

Description

TECHNICAL FIELD The present invention relates to a hypoglycaemic agent and a granule type hypoglycaemic agent,

The present invention relates to a method for producing a granule exhibiting a hypoglycemic effect using a natural product, and more particularly, to a method for producing a granule, And a method for producing the granule.

Recently, the incidence of diabetes mellitus is soaring in Korea as much as the diabetes mellitus. In the 1970s, the incidence of diabetes increased from 3% to 10% in the 1980s, with about 1% to 3% of the total population. By 2000, about 10% of the entire population was diabetic. Of those, Diabetes mellitus is a progressive type of diabetes currently underway.

 Especially in the late 2000s, there is a rapid increase in the number of hypertension and diabetes patients in a rapidly aging society. However, the currently implemented methods (such as hypoglycemic agents, insulin injections, and meal control methods) are difficult to cure. In other words, these methods are a kind of first aid measure that simply prevents the blood sugar from rising, and if you do not take the hypotensive, your blood sugar will rise again. However, at this time, this is the only way to prevent other complications, so you need to manage your blood sugar well in this way.

It is important to emphasize that it takes a long time to manage diabetes mellitus steadily over a period of 1-2 years, considering that diabetes has already started to progress three to five years ago. Most people with diabetes these days are in urgent need of blood sugar drops even after a few days of ingestion, but it is not easy to obtain such effects by using natural products using plants.

Diabetes is a disease in which the food we ingest is broken down into glucose and is not delivered to every part of our body (cells) that needs nutrients, and excreted by urine. Type 1 diabetes (congenital: 5%) refers to the inability to secrete insulin due to the destruction of pancreatic cells. Type 2 diabetes (acquired: 95%) causes insulin resistance and relative insulin deficiency, . As shown in the table below, the normal blood glucose level is determined to be diabetic if the fasting plasma glucose level is less than 110 mg / dl and the fasting glucose level is 125 mg / dl and the fasting glucose level is 126 mg / dl or higher in the ongoing glucose intolerance state.

The most common cause of diabetes is insulin secretion inhibition in the pancreas, but more fundamentally, it involves 6 parts of the body (liver, kidney, pancreas, heart, lung) (stomach, intestine, large intestine, bladder, ), And blood pressure and blood sugar gradually rise. In particular, if the functions of the pancreas, kidney, and liver are significantly reduced, the blood sugar increases and the diabetes progresses faster.

Diabetes mellitus is not only due to abnormalities of the pancreas but it is caused by multiple problems such as kidney, liver, stomach, and vascular disease.

The insulin secretion in the pancreas, the increase in blood sugar, or insulin secretion is not functioning in the absence of action, and the pancreas secretes insulin secretion of insulin is not produced by the breakdown of the phenomenon that the insulin is not produced, And clogging of the diabetes mellitus are all causes of diabetes mellitus. However, since these are the phenomenological, surface, and direct causes of diabetes mellitus, eliminating these causes does not help to cure diabetes mellitus.

Korean Patent Laid-Open Publication No. 10-2012-0109061 discloses a drug for preventing or alleviating blood glucose levels and diabetes, which comprises an enzyme-transformed yam extract as an active ingredient, and a method for producing the same. However, such a drug also has a temporary blood glucose- to be.

In order to cure diabetes, it is important to artificially lower the blood glucose level, which is the current method, to a normal level, but more importantly, it is necessary to treat and normalize the function of the damaged part of the pancreas, It is more important than anything else. If the function of Chapter 5, Part 6 is normal, the diabetic level will remain normal and at the same time complications will be prevented and diabetes can then be cured and cured.

Korean Patent Publication No. 10-2012-0109061

The present invention, as a conventional problem, was confirmed to have a level of 130 to 140 mg / dl even when the blood sugar level never reaches or reaches the normal value of 110 mg / dl even after ingestion by the method using the mixed herbal composition. It is a technical object of the present invention to provide a method for maintaining a blood glucose level below 110 mg / dl using a mixed herbal composition. In addition, many of the conventional invented mixed herbal compositions have not changed or even increased in the value per test result, and even though the blood sugar is somewhat lowered, the normal value can not be reached. Although it reached 130-140 mg / dl, it was judged to be the best method. At the same time, existing methods have shown that the problem is that the diabetes is fundamentally treated and not finally cured, and that it has no effect on the already complicated diabetes. The present invention relates to a method for treating diabetes mellitus within a period of from one month to three months after the administration of the diabetes mellitus within a period of one month to three months while at the same time treating the diabetes mellitus within a few months, It is an object of the present invention to provide a therapeutic composition.

The hypoglycemic agent of the present invention is a hypoglycemic agent comprising 15-30% of Hwanggi, 10-15% of Baekjang, 10-15% of Pueraria, 10-15% of Baengsam, 8-15% of Baekjang, 10-15% 5 to 10%, Hwangjeong 5 to 10%, Corn oil 5 to 10%, Sukjihwang 3 to 5%, Corn bean 2 to 5%, Licorice 1 to 5%.

The method for producing a blood glucose lowering granule according to the present invention comprises

10-15%, 10-15%, 10-15%, 10-15%, 10-15%, 8-15%, 10-15%, 5-15%, 5 ~ 10%, and 5 ~ 10%, respectively. 5 to 10% of corn oil, 5 to 10% of corn oil, 3 to 5% of corn steep liquor, 2 to 5% of corn mustard, and 1 to 5% of licorice, and then cleaned with distilled water. hr to obtain herbal extracts;

To preserve the drug efficacy of the obtained herbal medicine extract, water is removed from the vacuum concentrator at 65 ° C or lower, the extract is dried in a drier at 85 ° C and finely pulverized to 200 or less in a pulverizer to obtain a herbal extract powder; And

In order to prepare granules, vitamin A, B1, B2, C, D, E, Niacine, folic acid and minerals such as calcium, zinc and iron were added to herbal extract powder. And further mixing the water and the alcohol in an appropriate ratio to prepare a herbal extract as a granule.

Further, the volume ratio of the water: alcohol (95%) is 5: 95.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above problems occurring in the prior art, and it is an object of the present invention to provide a diabetic granule preparation, Reaching 110 mg / dl, and slowly reaching 110 mg / dl within 90 days. At the same time, daily blood glucose changes were kept constant, which improved the function of the damaged part of the body, which was caused by diabetes, and strengthened the capillary blood vessels and facilitated the blood flow disorder, which is important for prevention and treatment of circulatory diseases such as hypertension and arteriosclerosis And at the same time by taking advantage of the function of the pancreas, diabetes, vision problems, headache, fatigue, and has the effect of radically improved.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a flow chart of the steps of a method for producing the present hypoglycemic granule.

Hereinafter, the technical idea of the hypoglycemic agent and the method for producing the hypoglycemic agent according to the present invention will be described in detail with reference to the accompanying drawings. The following detailed description is intended to illustrate the technical spirit of the present invention only and should not be construed as limiting the technical idea of the present invention.

A typical symptom of diabetes is the phenomenon called 三 三 一 少. That is, it is a multifunctional food with a lot of food, a polyuria with a lot of urine, followed by drinking lots of water, and weight loss. In fact, the next time, diabetes mellitus should be very high blood sugar concentration is clear, while the body is generally healthy for the disease is misdiagnosed as a disease and does not recognize. Weight loss is an illness that is misdiagnosed as a diet for the elderly, and when the symptoms of diabetes and dryness are very severe, they feel sick and seek a doctor. According to statistics, about 57 percent of patients with type 2 diabetes mellitus have no symptoms, and about 35 percent have weight loss patients. In fact, only 8 percent of patients with diabetes mellitus have the following.

As age increases, we can not change aging and genetic factors, but we can see that other factors can be controlled through self-management. For example, regular exercise every day, the body should be tempered, abstinence of food, eating a lot of vegetables, eating less animal fat, and preventing obesity. Especially sugar (carbohydrate) absorption is moderate, and daily life is not too tense, stress-reducing, and anger. Also, you should refrain from drinking, smoking, watching TV, playing computer games late at night, drinking lots of warm water, and avoiding nervous emotions as much as possible. Particularly, it is a shortcut to diabetes, such as fatty pork belly, sausage, chicken, pizza, and western high calorie snacks. After drinking shochu in the pork belly, the air in our body is rapidly increased in the triglyceride level due to the carbohydrates in the rice, and when the state of high level is maintained repeatedly, blood stains such as blood clots (peach) Resulting in damage to the artery sclerosis. Especially rice has high triglyceride level, but saturated fat and alcohol in the meat can increase the triglyceride so that it can not be compared with rice. Therefore, the number of times should be reduced as much as possible.

However, in addition to these factors, the present invention has found the root cause of elevated diabetes levels.

First, it is a deterioration in function of 5 to 6 parts.

In addition to insufficiency of insulin secretion in the pancreas and inability to function after secretion, there is a problem in the function of the liver, kidney and blood vessels, but the sugar level is not high and the blood sugar is not falling well. Blood sugar levels can be kept normal. If the blood glucose is maintained at normal levels over a prolonged period of time (about 18 months or more), the pancreas is also very likely to recover slowly. Therefore, it is very important to maintain normal blood sugar control, and at the same time, if the function of the pancreas and other organs is restored, it can be said that the treatment and cure of diabetes is not impossible.

 In the previous studies, it was found that the initial glucose tolerance period was at least 6 to 18 months, depending on the degree of diabetes mellitus and individual differences. The reason for this is that when one of the five or six parts of the diabetes is damaged, it develops gradually and it takes three to five years for the diagnosis of diabetes. On the contrary, similar time is required for the treatment. However, in the present invention, the period of reaching the normal value was short, and the numerical value was maintained at 110 mg / dl or less.

 In conclusion, the causes of elevated glucose levels are not only the problems of the pancreas but also the hypertension (blood vessel cholesterol), kidney (kidney failure and kidney dialysis), liver (fatty liver, liver cirrhosis) When the function is lowered, the blood sugar increases and diabetes develops. Therefore, simultaneous ingestion of hypoglycemic agents (including insulin injections and insulin pumps) and NTB-A extract prescribed by the hospital causes the blood sugar to gradually decrease (blood glucose starts dropping from 20 days to 3 months depending on the patient) do. The meaning of the treatment is that if the blood glucose level before the intake was 300, the blood glucose level during the intake was maintained at 150, and if the blood glucose level did not increase, the blood glucose level was 200. Here, in order to fall to 110, as described above, the blood sugar does not drop unless the function of 5 parts or 6 parts is improved. The duration of this cure can vary from one year to 22 months, depending on the diabetic patient.

 The NTB-A extract must be scientifically well-blended with dozens of natural ingredients such as Puerariae radix, Hwanggi, Seokjanghwang, Sajamdang, Baekwoo, Baekjilip, At the same time, the amount of the compound is very important. In extracting method, the extracting time and temperature are precisely controlled using nanotechnology so that the major components are not destroyed.

Second, it is an irregular diet and kind.

Meals should be regular and non-stopping, and substituting bread or instant meal is a contraindication. So that the normal insulin secretion eventually becomes impossible. At the same time, eating a lot of meat or overeating is a bad habit of eating in half a bowl. If the plant does not have proper nutrition because of water or nutrition, it will manage the blood sugar as if it is dry, so that the body will not function properly and the aging will progress quickly and the diabetic complication will accelerate at the same time.

If the pancreas is managed only by the fear of diabetes, other organs will be damaged, so it must be managed in three dimensions.

It should be noted that the method of small amounts of food provided to diabetic patients is a very erroneous method. As an example, "diabetes is difficult to cure unless you eat properly." Therefore, if you eat vitamins and minerals that are essential for diabetic patients to a mixed herbal composition that is well-balanced with limited nutrients, the glucose level falls to normal in a short time, "It is necessary to take a balanced diet of vitamins and minerals in a balanced manner," he said. Reproductive forms of dietary supplements or food for diabetic patients are alternatives.

Third, lack of exercise and stress.

After meals, I would like to recommend more than 30 minutes of exercises that can sweat, such as walking or cycling. In particular, you should exercise sweating more than three times a week. People with diabetes need to exercise daily, exercise after cycling, and ride bicycles. Pre-eclampsia is not high, but postprandial blood glucose is high because the liver and pancreas are weak.

The hypoglycemic agent according to the present invention will be hereinafter also referred to as NTB-A herbal medicine extract.

The hypoglycemic granule according to the present invention is composed of Hwanggi, Sanjak, Changgeun, Samshan, Baekjung, Semi-Enlion, Byeongryeong, Kangwha, Hwangjeong, Sansui, Seokgi, Corn bean, Licorice and uses the function of damaged pancreas to produce insulin secretion Normal, and especially the damaged part of the pancreas other than the 5, 6 to restore normal function to cure the root cause of diabetes. Diabetes mellitus is normal if 5 (stomach, small intestine, bowel, bladder, gallbladder, three seconds) is normal in 6 parts (liver, kidney, pancreas, heart and lungs) In addition, the NTB-A herbal medicine extract of the present invention is prepared as granules containing vitamins and minerals, which are essential nutrients for diabetic patients, after being extracted three times for 3 hours at 100 ° C or less, concentrated, dried and pulverized.

The blood glucose lowering agent and the granule preparation method according to the present invention will be described in detail as follows. That is, the hypoglycemic agent of the present invention can be used as a hypoglycemic agent in an amount of 15 to 30%, 10 to 15%, 10 to 15%, 10 to 15%, 10 to 15%, 8 to 15%, 10 to 15% And 5 ~ 10% of corn steep liquor, 5-10% of corn steep liquor, 5 ~ 10% of corn steep liquor, 3 ~ 5% of steamed corn steep liquor, 2 ~ 5% of corn steep liquor and 1 ~ 5% of licorice liquor and cleaned with distilled water. When extracted three times within 3 hours once, it becomes NTB-A herbal medicine extract. (Extraction step)

In order to preserve the efficacy of the extracted NTB-A herbal medicine extract, water is removed from a reduced pressure concentrator at 65 ° C or lower, and the extract is dried in a dryer at 85 ° C and finely pulverized to a size of 200 or less in a pulverizer )

The granulated product thus prepared is granulated as follows: (granule preparation step)

In order to produce granules, calcium carbonate (Ca), zinc (Zn), calcium carbonate (Ca), zinc (Zn) Iron (Fe) is added and mixed. However, in the case of mixed herbal medicine extracts extracted as in the present invention, it is impossible to prepare granules by using water. In order to prepare pills, 50% It is necessary to contain a crushing mixture in an amount of at least% by weight to prepare a pellet. In the present invention, a technique for producing granules using an appropriate ratio of water and alcohol for the preparation of granules has been established. Particularly, it is possible to manufacture granules with only pure extractor powder without any additional binder such as starch used in pills or granules. To prepare the granules, a mixing ratio by volume of water: alcohol (95%) was added at a ratio of 5: 95.

In order to achieve the above object, the main ingredients of the diabetic granule according to the present invention are 15 ~ 30% of Hwanggi, 10 ~ 15% of Sajik, 10 ~ 15% of Pueraria, 10 ~ 15% , 15 ~ 5%, 5 ~ 10%, 5 ~ 10%, 5 ~ 10%, 5 ~ 10%, 3 ~ 5%, corn beard 2 ~ 5% and licorice 1 ~ 5% respectively Using the function of the pancreas other than 5 part 6, the diabetes level drops to keep it normal, does not rise again.

Hwanggi (黄耆, Astragali Radix) is a perennial herbaceous plant of the genus Fabaceae, about 1 meter in height, and its leaves are oval and oval. Beta, beta-sitosterol, formononetin, isoliquiritigenin, calcium, choline, copper, essential fatty acids, iron, magnesium, manganese, potassium and zinc. Pharmacological actions such as rejuvenation, cell regeneration, cardiopulmonary revascularization, and increase the facial force. Regarding the strengthening of blood sugar, it replenishes the blood, strengthens heart, lungs, regenerates cells damaged by diabetes, and increases immunity.

Diacoreae Rhizoma is a perennial plant of the genus Dioscorea japonica, about 1 meter tall and has three wings with yellowish gray color. The bulbous stem of the bat is used as a tonic, because it is a lance. The sticky ingredient is a glycoprotein, and it has many enzymes that help digestion. They are distributed in South Korea, South and Central Japan, and Japan. It contains 2-3% mucinous mucin of glucoprotein and produces mannose, glucose and 16 other amino acids by hydrolysis. The main pharmacological actions protect the spleen, stomach, lungs and kidneys. Regarding blood sugar enhancement, it protects the pancreas (spleen), stomach, lungs, kidneys, clears the urine, and strengthens the function of the pancreas cells, thus normalizing insulin secretion.

Puerariae Radix is a dry root of Pueraria thunbergiana (P. lobata), a perennial vernacular belonging to the legume, and its weakness is sweet, sour, It shows the efficacy of entering into the stomach and into the stomach and the septum, suzin, ginseng, and so on. Pharmacological actions include the action of glycosylation and body drainage, the relief of hyperactivity, the antipyretic action, the hypotensive action, the memory enhancement, the increase of the cerebral blood flow, the coronary artery dilation, the cardiac function improvement, and the antiarrhythmic effect. It is a symptom of diabetes that suppresses the following symptoms of dryness and thirst: it improves sugar function, improves heart function and lowers blood pressure.

Fourth is the pulmonary tuberculosis, which is a complication of diabetes, and it treats the polyuria, which is caused by pneumonia, swelling of the kidney, inflammation and frequent urination. In particular, liver and kidney function abnormalities to improve the symptoms of weak legs and strength.

It improves the function of the stomach damaged by diabetic complications.

The name of the raw material of the Semi-lotus (Semi-lotus) is chrysanthemum (rose moss) and is the origin of South America. The stem is reddish, branches are spread widely and is about 20cm high. Leaves are fleshy, slender, long cylindrical, with white hairs on the axilla. The flower has one or two petals on the end of branch, 2.5cm in diameter, with two calyx pieces and five petals. The petals are tapered, with red, yellow, white, and double flowers. The flowers bloom in July-October, bloom on a clear day, and bloom around 2 pm. The calyx is two ovate, membranous, with five petals, inverted egg shape, and the tip is digged. There are many stamens and there are 5 to 9 pistil heads in style. Fruits are capillary with membranes and ripen in September and split horizontally in the center to yield many seeds. The outposts contain alkaloids, flavonoids, and phenols. The main components are portulal, betanin, betacyanin, betanidin, and the like. It is known. Major pharmacological actions include eliminating heat from the lungs, stomach, and liver of diabetes, and eliminating the toxins that are distributed in Chapter 5 and 6. It removes heat and poisons from the body caused by diabetes, and especially removes bacteria caused by diabetes. Diabetes mellitus caused by microorganisms killed by the germs is also helpful in the treatment of diabetes.

Byeongryong removes various kinds of sediments (moss) in the kidney.

It breaks down the sugars present in the stomach, kidneys, liver, and blood vessels.

Hwangjeong eliminates skin irritation caused by diabetes (skin itching caused by peripheral nerve necrosis), cleanses blood, and decomposes sugar components in kidneys, stomach, and blood.

Corn oil protects the liver and kidneys, sprouts regularly, regenerates damaged cells due to diabetes, and carries out glucose degradation. It restores fragile liver function, especially in diabetic patients. When the liver function is weakened, it often develops sweating, occasionally covers or colds, and Lee Myung - Hyun 's voice appears in the ear, which improves the symptoms and strengthens the ovary.

Sukjihwang has the function of restoring the sexual function with the blood, the blood and the bosin, and treating the adversity.

Corn beard treats nephritis, makes urine come out well and lowers blood pressure. In particular, it strengthens the soy sauce to facilitate the action of sugar in the liver.

Licorice functions as a medicine in the present invention and acts to draw medicine to penetrate to the place where the illness exists.

This herbal extract is absorbed through the stomach wall, veins and capillaries to restore vision and control the endocrine function of each organ such as heart, liver, pancreas, kidney, lung, and stomach, It specifically enhances pancreas and kidney function. In addition, it releases toxins accumulated in each organ, and dissolves and releases toxins and sediments, especially in pancreatic cells, to promote insulin secretion.

Example 1: Preparation of NTB-A mixed herbal extract

In order to attain the above object, the present invention provides a method for producing NTB-A mixed herbal medicine extract, which comprises 15-30% of Hwanggi, 10-15% of Baechu, 10-15% of Pueraria, 10-15% Rice flour 10 ~ 15%, bamboo flute 2 ~ 5%, corn flour 5 ~ 10%, hwangjung 5 ~ 10%, corn oil 5 ~ 10%, wheat flour 3 ~ 5%, corn beard 2 ~ 5% , Distilled water is added, and the mixture is extracted at a temperature of 100 占 폚 for 3 hours or more. After the extraction, it is filtered, then distilled water is added again, and the same filtration is performed twice. The filtrate is combined and the water is removed from the vacuum concentrator at 65 ° C or lower in order to preserve the efficacy for the purpose of concentration. The extract is dried in a dryer at 85 ° C and then finely pulverized to a size of 200 or less in a pulverizer step)

Example 2: Preparation of NTB-A mixed herbal granule

B1, B2, C, D, E, Niacine, folic acid, and inorganic compounds, which are essential nutrients for diabetic patients, are added to 100 kg of NTB-A mixed herbal extract prepared in Example 1 Appropriate amounts of calcium (Ca), zinc (Zn) and iron (Fe) are added and mixed in a mixer. To this mixture, 5% v / v%: 95 v / v% of water (alcoholic alcohol, 95%) was added to the extract, and granules were prepared in the granulator.

Example 3: Hypoglycemic effect of granule of NTB-A mixed herbal composition

In order to examine the therapeutic effect of the mixed NTB-A extract of the present invention on diabetic cure and diabetic complication, the following experiment was conducted.

1. Experimental animals

Male rats of 200-230 g SD group at 7 weeks of age were fed with solid diet and water for two weeks before the start of the experimental diet and adapted to the breeding environment. Experimental animals were randomly assigned to a control group (NC), a diabetic control group (DC), a mixed medicinal granule (NTB-A ext) and a diabetic group (DC + S1 ).

Experimental animals were maintained at room temperature of 20 ± 2 ℃, relative humidity of 50 ± 10%, darkness of 12 hours (8:00 am to 8:00 pm), ventilation frequency of 10 to 20 times / hour and illumination of 150 to 300 Lux) Respectively. During the test period, the temperature and humidity of the animal room were automatically controlled by constant temperature humidity, and the environmental conditions such as illumination were regularly measured and there were no changes that affected the test.

2. Streptozotocin induced diabetes and diabetic complications

Streptozotocin-induced streptozotocin (STZ) induced diabetic and diabetic complications by intraperitoneal injection of 55 mg per kg body weight. After 48 hours of diabetic induction, the test animals were fasted for 12 hours. Blood was collected from the tail vein using a blood glucose meter (Accu-Check Active, Roche Diagnostics, Germany) and only those with a blood glucose level of 200 mg / Respectively. STZ is a chemical substance that selectively breaks down beta cells and causes hyperglycemia due to insulin deficiency, which has been used to induce diabetes. Especially, Single High Dose Streptozotocin (SHDS) irreversibly induces sudden hyperglycemia due to mass necrosis of beta cells and induces irreversible hyperglycemia, which is suitable for the diabetic complication model.

3. General observations (changes in body weight, feed and intake)

Weight change was observed at intervals of one week, and the body weight of the first day of the sample was taken as the initial body weight, and the body weight was determined as the final weight after 6 weeks. The actual dietary intake was the amount obtained by subtracting the shed amount from the dietary intake, and the total dietary intake was the dietary intake for 6 weeks.

In order to examine the therapeutic effect of chronic diabetic complications, the drug was administered for about 8 weeks from the 31st day after continuing the 30 day diabetic state in the rat.

4. Blood sampling

The initial blood was obtained from the tail vein after fasting the test animal 12 hours before the start of the drink supply. The animals were fasted for 12 hours on the last day of the 6th week, and urethan (3 g / kg body weight) was injected into the abdominal cavity and anesthetized to collect blood from the abdominal cavity of the experimental animals. The collected blood was allowed to stand at room temperature for 30 minutes and then coagulated. The serum was separated by centrifugation at 6,500 rpm and 4 ° C for 20 minutes. The separated serum was stored at 30 ° C.

5. Measurement of sugar and lipid content in serum

On the first day of the test, blood glucose, triglyceride, total cholesterol, and HDL-cholesterol were tested for the serum obtained from the tail vein and the end-stage celiac vein.

6. Statistical processing

All test results were processed according to the Levene's test. Statistical analysis was performed by one-way analysis of variance (ANOVA) method. Student's t test was used to test for the difference between the control and experimental groups at p <0.05 level.

7. Test Results

7.1 Weight change

Table 1 and Table 2 show the changes in body weight, amount of increase, dietary intake, and blood glucose drop during the experimental period after induction of diabetes.

As shown in Table 1, the weight change was steadily increased in the normal control group for 6 weeks, while the diabetic group showed a decrease in body weight continuously during the experimental period and showed a lower body weight than the initial body weight at the end of the experiment. This suggests that STZ selectively destroys the pancreatic β-cells, resulting in abnormal insulin production, and imbalance of glucose metabolism, resulting in failure of body weight recovery. At the same time, the body weight of the normal group was increased by 91.8 g, but the diabetic group was decreased by 37.2 g. In addition, 24.7g of the group administered with the mixed herbal composition granules (DC + NTB-A ext) was reduced in the induced group, and 21.9g in the positive control group (DC + S1) in the diabetic group. As described above, the group administered with the herbal composition of the present invention showed less decrease in the gestational age than the diabetic group, but it was found to be higher than that of the diabetic group. Diabetes - induced diabetes mellitus group received about 1.8 times more diets than normal diabetes group, and diabetic control group received more diabetes mellitus than normal group. However, the mixed herbal composition administration group of the present invention consumed slightly less amount than the diabetic group, and the diabetic group consumed 5 times more water than the normal group. At the same time, the mixed herbal composition administration group of the present invention consumed about 2.5 times less water than the diabetic group, and the diabetic group had more water than the diabetic group.

NTB-A ext Diabetic agent Diabetic rat 6 week weight change   group                 Weight (g)   Initial (g)   The terminal period (g) Weight gain (g) NC 310.5 ± 5.5 402.3 ± 8.3 91.8 DC 290.6 + - 8.4 253.4 + - 10.2 -37.2 DC + NTB-A ext 289.9 ± 7.5 265.2 ± 9.9 -24.7 DC + S1 291.4 ± 9.5 269.5 ± 5.8 -21.9

※ NC + CMC; Normal group + carboxymethylcellulose

   DC + CMC; Diabetic group + carboxymethylcellulose

   DC + NTB-A ext; Diabetic induction group + mixed herbal composition granule

DC + S1; Diabetic group + positive control group (epalestate)

7.2 Glycemic effect

7.2-1 Experimental rat

Blood was taken from the orbital vein of the STZ-induced diabetic group and glucose was measured using a glucose kit. As shown in Table 2, the blood glucose level in the serum was found to be 403.8 ± 25.2 mg / dl in the diabetic group, and 138.54 ± 52.4 mg / dl in the NTB-A mixed extract group of the present invention, The blood glucose lowering effect is excellent. And 133.45 ± 33.5 md / dl in the positive control group (DC + S1).

Hypoglycemic effect of NTB-A extracted mixed herbal composition (experimental rat)  group             Blood sugar level (mg / dl) NC + CMC DC + CMC DC + NTB-A ext DC + S1 Initial 112.2 ± 3.2 375.9 ± 25.7 346.8 ± 6.8 364.22 ± 27.4 Final (6 weeks) 109.3 ± 3.7 403.8 ± 25.2 138.54 ± 52.4 133.45 ± 33.5

  ※ NC + CMC; Normal group + carboxymethylcellulose

      DC + CMC; Diabetic group + carboxymethylcellulose

      DC + NTB-A ext; Diabetic induction group + mixed herbal composition granule

DC + S1; Diabetic group + positive control group (epalestate)

7.2-2 People

The blood glucose lowering effect of the NTB-A extracted mixed herbal composition of the present invention is shown in Table 3.

Hypoglycemic Effect of NTB-A Extract Mixed Herbal Composition (Human)  Intake period Group A (mg / dl) Group B (mg / dl) Group C (mg / dl) Group D (mg / dl) Group E (mg / dl) Group F (mg / dl)   Before ingestion 230 ~ 250 210-235 250 to 270 280-320 280 ~ 330 250 to 270  1 week average 190 ~ 220 174-205 230 ~ 255 252 to 286 255 ~ 301 240 ~ 267  2 week average 150-175 153-175 210 ~ 233 237 to 256 233-278 234 to 257  3 week average 130 ~ 144 133-150 185 ~ 209 210 ~ 231 215 to 244 210 ~ 249  4 week average 105-110 108 ~ 113 155-180 183-201 180 ~ 219 195-233  6 week average  95 ~ 106 102-109 130 ~ 153 152-179 160-190 171-201  8 week average 104-108 101 ~ 105 109-131 137-137 150-175 145-178 10 week average 102 to 107 103-106 108 ~ 123 125-137 138 ~ 156 133 ~ 158 12 week average 105 ~ 109 106 ~ 109 105-110 115 ~ 129 125 ~ 138 128-141

Group A; 10 males aged 40 to 50 years, group B; 10 women, 40 to 50 years old, group C; 10 males aged 50 to 60 years, group D; 10 men 60 to 70 years old, group E; 10 males over 70 years old, group F; 50 to 60 years old Insulin to female 10.

8. Diabetic rat test results after 6 weeks

BUN (Blood Urea Nitrogen), an index of renal function, was measured by absorbance at 580 nm using the urease-indophenol method, which was about 2.4 times higher in the diabetic group than in the normal group And the NTB-A mixed herbal composition administration group of the present invention was significantly lower than the diabetic group (p <0.05).

In addition, triglyceride was significantly increased in the diabetic induced group, and the mixed herbal composition administration group of the present invention was significantly decreased (50.55 ± 15.77 mgldl; p <0.05) as compared with the diabetic induced group.

Serum test results of diabetic rats after 6 weeks BUN (mg / dl) Triglyceride
(mg / dl) Total cholesterol (mg / dl) HDL
  NC + CMC 16.54 ± 0.55 33.56 ± 5.99 64.21 + - 10.76 56.0 ± 4.1  DC + CMC 34.21. + -. 3.99 71.08 ± 12.78 54.45 + - 11.56 46.7 ± 12.1  DC + NTB-A ext 25.12 + - 3.55 50.55 + 15.77 53.54 + - 7.23 49.9 ± 5.1  DC + S1 22.54 ± 5.18 47.32 + - 7.98 59.55 ± 12.66 50.3 ± 6.5

     ※ NC + CMC; Normal group + carboxymethylcellulose

        DC + CMC; Diabetic group + carboxymethylcellulose

        DC + NTB-A ext; Diabetic induction group + mixed herbal composition granule

DC + S1; Diabetic group + positive control group (epalestate)

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not intended to be exhaustive or to limit the invention to the precise forms disclosed. It will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.

Claims (2)

10-15%, 10-15%, 10-15%, 10-15%, 10-15%, 8-15%, 10-15%, 5-15%, 5 ~ 10%, and 5 ~ 10%, respectively. 5 to 10% of corn steep liquor, 5 to 10% of corn steep liquor, 3 to 5% of steamed corn steep liquor, 2 to 5% of corn steep liquor, and 1 to 5% of licorice. 10-15%, 10-15%, 10-15%, 10-15%, 10-15%, 8-15%, 10-15%, 5-15%, 5 ~ 10%, and 5 ~ 10%, respectively. 5 to 10% of corn oil, 5 to 10% of corn oil, 3 to 5% of corn steep liquor, 2 to 5% of corn mustard, and 1 to 5% of licorice. Clean and wash with distilled water. 3 times to obtain herbal extract;
To preserve the drug efficacy of the obtained herbal medicine extract, water is removed from the vacuum concentrator at 65 ° C or lower, the extract is dried in a drier at 85 ° C and finely pulverized to 200 or less in a pulverizer to obtain a herbal extract powder; And
Vitamin A, B1, B2, C, D, E, Niacine and folic acid were mixed with calcium (Ca), zinc (Zn) and iron (Fe) And a step of preparing a crude drug extract as a granule by further mixing the drug with the alcohol,
Wherein the volume ratio of the water to the alcohol (95%) is 5: 95.

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