KR20150105550A - Cosmetic composition comprising mucus extract from spawn - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a mucus extract of animal spawn selected from a snail spawn, a pond snail spawn and a frog spawn. The cosmetic composition of the present invention has excellent anti-oxidant, anti-wrinkle, skin moisturizing, skin barrier function restoring, atopic dermatitis treating, and anti-inflammatory effects.

Description

The present invention relates to a cosmetic composition comprising a mucus extract of animal eggs,

The present invention relates to a cosmetic composition containing an mucus extract of animal eggs, and more particularly to a cosmetic composition containing mucilage extract of animal eggs selected from snail eggs, coagulant eggs and frog eggs.

The extent to which aging of cells affects the aging of individuals (humans, animals) is not yet known. It is well known that the telomere at the end of the chromosome is shortened each time it breaks down and the cell dies when it reaches its limit. Recently, there has been research that collagen, which is the main component of cell epilepsy, is reduced to 65% of the 20s when it is in their 80s and is involved in aging. There are also studies that abnormal protein accumulation due to intracellular ER stress is involved in aging.

Skin aging can be divided into two types: intrinsic (chronological aging) and phtoaging (Gilchrest BA: J. Am . Acad . Dermatol ., 21, 610-613 (1989)]. Endogenous aging is a naturally occurring aging phenomenon that is accompanied by decreased physiological function of the body as age increases [Braverman IM et al . : J. Invest . Dermatol ., 78, 434-443 (1982)]. Photoaging means that the skin is repeatedly exposed to light to change the appearance or function of the skin [Ridder GM et al . : J. Am . Acad . Dermatol ., 25, 751-760 (1991)]. In addition to the above, skin aging can be caused by active oxygen species activated by ultraviolet rays, stress, disease states, environmental factors, wounds, and aging. When such conditions are deepened, the antioxidant defense network existing in the living body is destroyed, And tissues to promote adult diseases and aging. More specifically, lipids, proteins, polysaccharides and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins causes severe hyperinflammation of collagen, hyaluronic acid, elastin, proteoglycans, and fibronectin, which are connective tissues of the skin, which interferes with skin elasticity. Mutation, cancer induction, and immune function.

Therefore, it is necessary to protect the cell membranes by abolishing reactive oxygen species that are mediated by the body's metabolic processes, ultraviolet irradiation, and inflammatory reactions, and regenerate already damaged cells by active metabolism to proliferate the cells Can be restored and healthy skin can be maintained. In aging, not only active oxygen species but also matrix metalloproteinases (MMPs) are involved. In vivo, synthesis and degradation of extracellular matrix such as collagen are appropriately controlled, but their synthesis decreases as aging progresses Expression of MMP, an enzyme that degrades collagen, is promoted, and skin elasticity is lowered and wrinkles are formed. In addition, these degrading enzymes are activated by ultraviolet exposure. Therefore, there is a demand for development of a substance capable of controlling the activation of MMP induced in the cell by ultraviolet light or inhibiting its activity. However, until now, raw materials used as cosmetic materials simply suppressed the activity of MMP.

Atopic dermatitis is a common disease in people with atopic allergies. Although the cause of atopic dermatitis has not yet been clarified, atopic dermatitis has a history of atopy in 70% of the patients, And it is understood to be one of diseases caused by genetic factors or immune diseases because it is accompanied by allergic diseases such as allergic rhinitis and asthma. In the treatment of atopic dermatitis, medicines such as corticosteroids, which can expect high pharmacological effects, are used, but side effects of corticosteroids are problematic, and the use of corticosteroids causes the rebound It is also well-known that a later severe worsening of the affected part occurs. As a countermeasure for such side effects, a non-steroidal anti-inflammatory agent or an antihistamine agent which does not contain a hormone such as adrenocortical hormone is used but it is very difficult to develop a remedy or therapeutic agent for atopic dermatitis without side effects because it is difficult to cure.

On the other hand, in the human body, the skin is an organ acting as a protective film for protecting the living body from the external environment. It prevents loss of biological components in the human body, that is, water and electrolytes, and prevents harmful substances from entering the human body from the outside . The skin is divided into the skin layer, the dermal layer and the subcutaneous fat layer. The epidermal layer consists of keratinocytes and melanocytes. Since the keratinocytes in the outermost layer of the skin are in direct contact with the external environment, they are required to have strong resistance against physical, chemical, or material permeation and prevent moisture from being lost to the outside At the same time as zooming, it should maintain its flexibility by holding proper water on its own.

Generally, the moisture content of the skin is about 70% in the dermal layer, but decreases to the skin layer, which is about 10 to 30% in the keratin layer. The water supplied from the dermis layer mainly migrates to the upper part of the stratum corneum by passive diffusion and eventually to the outside. This is called Trans Epidermal Water Loss (TEWL). It is known that the lipid component of the keratinocyte layer and epidermal lipid, which maintains the transdermal water loss at an appropriate level, is the lipid component.

On the other hand, it is known that the keratinocyte layer has a hydrophilic substance called a natural moisturizing factor (NMF) and plays an important role in skin moisturization. Normal keratinocyte layer maintains skin smoothness and softness and maintains normal body protection function when moisture is maintained at about 10 to 30%. However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the protective function of the body is lost and the aging phenomenon occurs. For example, in the case of dry skin, the keratinocyte aggregation is weakened, and a scaling phenomenon appears in which the keratinocytes are peeled off like scales from the skin surface. The reason why the skin is dried in this way is that the moisture content of keratinocyte layer is smaller than that of normal skin. In addition to this, even healthy skin may be in a harsh environment, such as wind, cold weather, sunshine, cleansing, shaving, etc., which causes water shortage, which can lead to poor skin condition.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer. To this end, cosmetics were added with ingredients similar to sebum, moisturizers such as NMF or polyol, and the like. For example, glycerin, solitol and the like having three or more hydroxyl groups (-OH) as water-soluble polyols exhibit excellent moisture resistance, but they are very sticky and feel uncomfortable when used. Propylene glycol having two hydroxyl groups (-OH) 3-butylene glycol, etc. may cause side effects of the skin. In addition, other natural moisturizing factors such as sodium pyrrolidonecarboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic, which deteriorates emulsion stability of the cosmetic. Amino acids, collagen, elastin, etc. are also capable of moisturizing, but also had a limited ability to moisturize.

The ability of the stratum corneum to retain moisture can be controlled by NMF, which consists of sebum components, amino acids, lactic acid, urea, and inorganic salts. It is safe and usable as a natural moisturizing factor in skin external preparations. The development of high-quality materials is one of the important research projects in cosmetics.

Meanwhile, in recent years, many cosmetic products using natural products have been developed to reduce skin irritation caused by various chemical substances and the like. In addition to low adverse effects on skin, natural materials have recently become increasingly appreciated as a cosmetic raw material as consumers' response to cosmetics using natural materials has increased.

On the other hand, eggs containing various fish eggs such as salmon, salmon, and tuna are known to be effective for diet because of abundance of proteins and minerals and low calories. There is already a lot of health foods containing these, , And studies of applying such mucus extracts of animal eggs to cosmetic compositions have not been conducted.

The present inventors have studied the applicability of various natural products to cosmetics. As a result, they have selected mucilage extracts of animal eggs and found that these mucilage extracts have antioxidant, skin wrinkle, skin moisturizing, restoration of skin barrier function, And confirmed the effect of inflammation, thereby completing the present invention.

Korean Patent Registration No. 10-0762953 (Registration Date: 2007.09.21) discloses a frog oyster tube extract showing hepatoprotective effect against acetaminophen-induced liver damage and a liver function-protecting agent containing the same. However, Does not disclose the use of frog egg extract as a composition for external application for skin.

It is an object of the present invention to provide a cosmetic composition for improving antioxidation, skin wrinkle, skin moisturizing, skin barrier function recovery, atopy improvement, and anti-inflammatory effect without causing side effects.

In order to achieve the above object, the present invention provides a cosmetic composition characterized by containing a mucus extract isolated from animal eggs as an active ingredient.

An embodiment of the present invention provides a cosmetic composition comprising a mucus extract of animal eggs selected from snail eggs, cochineal eggs, frog alo eggs.

In one embodiment of the present invention, there is provided a cosmetic composition characterized in that the mucus extract is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition.

In one embodiment of the present invention, the mucus extract is obtained by washing the animal egg, disinfecting or sterilizing the animal, disintegrating the egg, and providing the cosmetic composition using the extract obtained therefrom.

One embodiment of the present invention provides a cosmetic composition for antioxidation, skin wrinkle improvement, skin moisturizing, skin barrier improvement, atopic improvement and / or anti-inflammation.

The cosmetic composition of the present invention is excellent in antioxidation, skin wrinkle, skin moisturizing, skin barrier function recovery, atopy improvement, and anti-inflammatory effect.

In addition, since the present invention uses a mucus extract of animal origin, which is a natural material, it is possible to provide a cosmetic composition which is harmless to human body and is excellent in safety.

The present invention provides a cosmetic composition containing mucus extract of animal eggs. The mucus extract of animal eggs applied to the present invention is not limited to specific animal species and is characterized by containing mucus extract obtained from all species as an effective ingredient, unless there is a special reason. A special reason is a case of limited use because it is listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) as a protected species.

In one embodiment of the present invention, the animal egg is preferably a snail egg, a snail egg, a frog egg, or the like. The mucus extract can be obtained by collecting animal eggs such as snail eggs, coagulant eggs, frog eggs, etc., washing them, disinfecting and sterilizing them, and then crushing and extracting the obtained products. At this time, it can be extracted with water, alcohol or acetone.

The cosmetic composition of the present invention may contain mucus extract in an amount of 0.001 to 30% by weight based on the total weight. Preferably, the mucus extract may be 0.01 to 15% by weight, more preferably 0.1 to 10% by weight, based on the total weight of the composition. When the mucus extract is contained in the above-mentioned range in the total amount of the cosmetic composition, the effect of the mucilage extract of animal eggs can be sufficiently exerted without deteriorating the quality suitability of the cosmetic composition.

The cosmetic composition according to the present invention may be used, for example, as any one of antioxidant, skin wrinkle, skin moisturizing, restoring skin barrier function, atopic dermatitis, and anti-inflammatory.

The cosmetic composition according to the present invention may contain other ingredients in addition to the mucilage extract of animal eggs within the range not impairing the main effect of the present invention.

Such other ingredients include, for example, hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents, fragrances, fillers, blocking agents, pigments, absorbers and dyes. The amount of the various adjuvants is an amount ordinarily used in the art, for example, 0.001 to 30% by weight based on the total weight of the composition. However, in any case, the adjuvants and the ratio thereof are selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

Meanwhile, the formulations of the cosmetic composition of the present invention are not limited to specific ones, and examples thereof include emulsions of lotion, gel, water-soluble liquid, cream, essence, oil-in-water type and w / A color cosmetic formulation consisting of an essential cosmetic formulation made of ointment, an underwater type and a water-in-oil type make-up base, a foundation, a skin cover, a lipstick, a lip gloss, a face powder, a two way cake, an eye shadow, a teak color and an eyebrow pencil One is good. It may also optionally be applied to the skin in aerosol form, in solid form, e.g. in the form of a stick, and as a skin care and / or makeup product.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Example  1: Preparation of mucilage extract of snail eggs]

Snail eggs within 10 days after spawning were selected, washed in clean running water, cleaned with 70% ethanol and sterilized.

The disrupted eggs were crushed and extracted into an aqueous solution of 30% (w / w) butylene glycol and 70% (w / w) water, filtered through 400 mesh, and then obtained a mucilage extract of snail eggs.

[ Example  2: Preparation of mucilage extract of coagulant eggs]

The mucus extract of coagulometer eggs was obtained in the same manner as in Example 1.

[ Example  3: Preparation of mucilage extract of frog eggs]

The mucilage extract of frog eggs was obtained in the same manner as in Example 1.

[ Experimental Example  One: NBT Antioxidant Effectiveness Measuring Experiment]

In order to measure the antioxidative effect of the extracts of animal mucus obtained in Examples 1 to 3, antioxidant activity was measured by NBT (Nitroblue terrazolium) method using the antioxidants such as retinol and BHT as comparative samples under laboratory conditions Respectively.

In order to measure the antioxidative effect, the active oxygen produced by xanthine and xanthine oxidase was measured by the NBT method and the effect of the test substance on the removal of active oxygen, that is, the effect of active oxygen scavenging was evaluated. The active oxygen scavenging rate was measured by measuring the blue color produced by the reaction of active oxygen with NBT at a wavelength of 560 nm by xanthine and xanthine oxidase.

As a measurement method, 0.1 ml of 0.05 M Na ₂ CO ₃ , 0.1 ml of a 3 mM xanthine solution, 0.1 ml of a 3 mM EDTA solution, 0.1 ml of a BSA solution and 0.1 ml of a 0.7 mM NBT solution were added to Bayer's disease and 0.1 ml of a sample solution And allowed to stand at 25 DEG C for 10 minutes. Thereafter, 0.1 ml of xanthine oxidase solution was added and stirred, followed by incubation at 25 ° C for 20 minutes. Then, 0.1 ml of 6 mM CuCl ₂ was added to stop the reaction, and the absorbance St at 560 nm was measured. In the blank test, distilled water was used instead of the sample solution, and the remainder was treated in the same manner as above to measure the absorbance Bt. In addition, the blank of the sample solution was subjected to the same operation using distilled water instead of the xanthine oxidase solution to measure the absorbance Bo.

The results are shown in Table 1 below. ≪ tb > < TABLE >

[ Equation  One]

Inhibition rate (%) = [1- (St-So) / (Bt-Bo)] 100

St: Absorbance at 560 nm after enzyme reaction of sample solution

Bt: Absorbance at 560 nm after enzyme reaction of blank test solution

So: the absorbance at 560 nm before the reaction in the absence of enzyme in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of enzyme in the blank test solution

Name of sample Treatment concentration (%) Antioxidative effect(%) Example 1 0.1 86 Example 2 0.1 84 Example 3 0.1 86 Retinol 0.1 88 BHT 0.1 84

As shown in Table 1, the antioxidative effect of the extract of the present invention was 84 ~ 86%, which is similar to that of retinol and BHT. .

[ Experimental Example  2: DPPH Antioxidant Effectiveness Measuring Experiment]

In order to measure the antioxidative effect of the extracts of animal mucus obtained in Examples 1 to 3, antioxidant activities such as vitamin E and green tea extract were compared with each other in DPPH assay Respectively.

DPPH is a method for measuring antioxidative activity by reducing power using a free radical called 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (DPPH). Free radical scavenging was measured at a wavelength of 560 nm by comparing the degree of reduction of the absorbance by DPPH reduction by the test substance with the absorbance of the blank test solution.

As a reagent used, 61.88 mg of a 0.1 mM solution of 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) was dissolved in methanol to make 100 ml. To 0.15 ml of a 0.1 mM DPPH solution, 0.15 ml of a sample solution was added to a 96-well plate, followed by rapid stirring, followed by incubation at 25 ° C for 10 minutes. Thereafter, the absorbance St at 560 nm was measured. The blank test was carried out in the same manner as above using distilled water instead of the sample solution, and the absorbance Bt was measured. Also, the blank of the sample solution is measured by the same procedure using methanol instead of the 0.1 mM DPPH solution to determine the absorbance Bo

The results of the antioxidant effect were calculated by the following equation (2), and the results are shown in Table 2 below.

[ Equation  2]

Inhibition rate (%) = [1- (St-So) / (Bt-Bo)] 100

St: absorbance at 560 nm after free radical scavenging of the sample solution

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of free radicals in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample Treatment concentration (%) Antioxidative effect (%) Example 1 0.01 52 Example 2 0.01 54 Example 3 0.01 55 Green tea extract 0.01 40 Vitamin E 0.01 37

As a result of the experiment, the animal mucus extract of the present invention showed an antioxidative effect superior to green tea extract and vitamin E at the concentration of 0.01%.

[ Experimental Example  3: in vitro MMP -1 inhibition evaluation]

In this experimental example, the inhibitory activity of substrate metalloproteinase (MMP-1) was measured in a biochemical model in order to measure the anti-aging effect of the extract of animal mucus obtained in Examples 1 to 3 above. (EnzChek® gelatinase / collagenase kit, Molecular Probes), based on the use of collagen and gelatin conjugated to purified collagenase and its substrate fluororesin.

The collagenase purified from Clostridium histiochem was supplied into the EnzChek (TM) gelatinase / collagenase kit. Purified from porcine skin and incubated with DQ-collagen conjugated to fluorescein and reaction buffer {0.05 M Tris-HCl, 0.15 M NaCl, 5 mM CaCl ₂ And 0.2 mM sodium azide (pH 7.6)} was used with EnzChek (TM) gelatinase / collagenase kit (Molecular Probes).

Animal alum mucus extract was dissolved in the above reaction buffer to prepare samples at 0.04% and 0.02% (w / v), and then used in the experiment. Each of the above samples was incubated at room temperature for 15 minutes, 45 minutes, and 120 minutes with 25 ug / ml of DQ-collagen and 0.1 U / ml of collagenase. Also, under the respective experimental conditions, the control mixture corresponding to the collagenase and DQ-collagen mixture was incubated in the same manner, and a sample called Blank, hereinafter referred to as "enzyme-free blank" - in the presence of collagen and in the absence of collagenase.

Each experiment was performed three times. After 120 minutes, the signal corresponding to the decomposition of DQ-collagen was measured with a fluorescence analyzer (excitation: 485 nm, emission 505 nm). Thereafter, the fluorescence value of each sample was measured based on the 'enzyme-free blank' fluorescence value. The results show the mean value of the fluorescence unit per sample and the percent variation (%) for the control group.

Experimental Sample Inhibition rate (%) Treatment concentration (%) 0.02 Treatment concentration (%) 0.04 Example 1 62 79 Example 2 64 80 Example 3 61 77 Retinol 0 9 Green tea extract 55 70

As a result of the experiment, as shown in Table 3, inhibition rates of 61-64% and 0.04%, respectively, when treated with 0.02% of the animal mucus extracts of Examples 1 to 3, showed inhibition rates of 77-80% , And it was confirmed that it possessed anti-collagenase / gelatinase activity in a dose-dependent manner.

In addition, the animal louse mucus extracts of Examples 1 to 3 were found to be superior to the MMP-1 inhibitory effect of green tea extract.

[ Experimental Example  4: After UV irradiation MMP -1 expression inhibition evaluation]

In this experiment, ELISA was performed to measure the concentration of MMP-1 after UV irradiation and sample addition of the animal slime extract obtained in Examples 1 to 3 above.

UVA was irradiated to human dermal fibroblasts at an energy of 5 J / cm < 2 > using a UV chamber. Ultraviolet irradiation dose and incubation time were established by preliminary experiment to maximize MMP expression level in fibroblasts. Negative controls were wrapped in silver foil and kept in the UVA environment for the same amount of time. UVA emission was measured using a UV radiometer. Cells while UVA was irradiated were irradiated with UVA, and then cultured for 24 hours in a medium supplemented with the sample. Then, the medium was recovered and coated on a 96-well plate. The primary antibody {MMP-1 (Ab-5) monoclonal antibody and MMP-2 (Ab-3) monoclonal antibody} was treated and reacted at 37 DEG C for 60 minutes. The secondary antibody, anti mouse IgG (alkaline phosphatase conjugated), was reacted again for about 60 minutes, and the alkaline phosphatase substrate solution (buffer solution of 1 mg / ml p-nitrophenyl phosphate in diethanolamine) was reacted at room temperature for 30 minutes And the absorbance at 405 nm was measured with a microplate reader. As a control group, a sample to which no sample was added was used.

Test group Treatment concentration (%) MMP-1 expression inhibition rate (%) Control group - - Example 1 0.1 23 Example 2 0.1 25 Example 3 0.1 22 Retinol 0.1 21

As a result of the experiment, as shown in Table 4, the MMP-1 in which the expression was induced upon irradiation with ultraviolet light showed an inhibition rate of 22 to 25% in the animal louse extract of Examples 1 to 3, , Which was superior to the inhibition rate of retinol used as a control.

[ Experimental Example  5: Inhibitory effect of inflammatory cytokine expression by ultraviolet irradiation]

This experiment evaluated the degree of suppression of the expression of inflammatory cytokines expressed by ultraviolet irradiation in order to confirm the anti-inflammatory effects of the extracts of animal mucus obtained in Examples 1 to 3 above.

Fibroblasts isolated from human epidermal tissue were placed in a 24-well test plate at 5 × 10 ⁴ and adhered for 24 hours. Each well was washed once with PBS and 500 占 퐇 of PBS was added to each well. After irradiating the fibroblasts with 10 mJ / cm2 of ultraviolet light using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15 W, Sankyo Dennki, Japan), PBS was removed and the cells were cultured in a cell culture medium ≪ / RTI > supplemented medium) was added. The animal mucus extract to be evaluated was treated and incubated for 5 hours. 150 [mu] l of culture supernatant was taken to quantitate IL-1 [alpha], thereby determining the effect of inhibiting the expression of inflammatory cytokines in the extract of animal louse mucus. The amount of IL-1? Was quantitated using an enzyme-linked immunosorbent assay (ELISA), and the production rate of IL-1? Was calculated by the following equation (3).

[ Equation  3]

Inhibitory rate of inflammatory cytokine expression (%) = [1- (St-Bo) / (Bt-Bo)] X 100

Bo: IL-1? Production in wells without UV irradiation

Bt: IL-1? Production in wells irradiated with ultraviolet light and not treated with the sample

St: IL-1α production in wells irradiated with ultraviolet light and treated with the sample

Name of sample Treatment concentration (%) Inhibitory rate of inflammatory cytokine expression (%) Example 1 0.01 31 0.02 64 Example 2 0.01 29 0.02 66 Example 3 0.01 32 0.02 59

As shown in Table 5, the animal louse extracts of Examples 1 to 3 inhibited the production of inflammatory cytokine IL-1α by 59 to 66% at a concentration of 0.02% , It was confirmed that the generation of inflammation due to ultraviolet rays was effectively prevented even at low concentrations.

[ Manufacturing example  1 and 2: extracts containing an extract of animal louse mucus Cosmetics  Composition Preparation]

The present Preparation Examples 1 and 2 were prepared by using the animal mucus extract prepared in Examples 1 and 2.

In order to prepare a cosmetic composition containing an animal's mucus extract, the (B) phase recorded in Table 6 below was heated and stored at 70 占 폚. (A) was added to the mixture, followed by preliminary emulsification, uniformly emulsified with a homomixer, and then gradually cooled to prepare a cosmetic composition containing creamy animal louse mucus extract. In addition, as shown in Table 6 below, a general cosmetic composition containing no animal-derived mucus extract was prepared and referred to as Comparative Example 1.

Raw material Production Example 1 Production Example 2 Comparative Example 1 end Stearyl alcohol 8 8 8 Stearic acid 2 2 2 Stearic acid cholesterol 2 2 2 Squalane 4 4 4 2-octyldodecyl alcohol 6 6 6 Polyoxyethylene (25 mole addition) alcohol ester 3 3 3 Glyceryl monostearate Aster 2 2 2 Liquid paraffin 5 5 5 I Example 1 (Snail's mucus extract) 5 - - Example 2 (Extract of mucilaginous mucus) - 5 - Propylene glycol 5 5 5 glycerin 4 4 4 Purified water Balance Balance Balance

Unit: wt%

[ Experimental Example  6: Skin wrinkles  Improvement effect measurement experiment]

In order to examine the effect of the cosmetic composition containing Snail's mucus extract of Preparation Example 1 for improving the skin wrinkles, the following Experimental Example was carried out as follows.

The creams of Preparation Example 1 and Comparative Example 1 were applied to 10 persons (20 to 35 years old female) of the experiment. The cream of Preparation Example 1 was applied to the right side of the face and the cream of Comparative Example 1 was applied to the left side of the face. The coating was applied continuously for 2 months. After the completion of the experiment, the effect of alleviating the skin wrinkles was measured by using a silicone resin copy (replica) before and after using the product for 2 months and comparing the state of the eye wrinkles with a skin micro-wrinkle device and a skin image analyzer.

Production Example 1 Comparative Example 1 Before use After 2 months use Before use After 2 months use Average fine wrinkle depth 0.187 0.158 0.190 0.182 Wrinkle reduction rate before use (%) 15.5% 4.21%

n = 10, p < 0.05

As a result of the experiment, it was found that the skin wrinkle improving effect was excellent in the skin around the eyes of the user who applied the cream of Preparation Example 1 containing Snail's mucus extract as shown in Table 7 above.

[ Experimental Example  7: atopy improvement effect]

In this Experimental Example, the cosmetic composition of Preparation Example 2 and Comparative Example 1 was evaluated by comparing the effect of improving atopy on a person having atopy.

Twenty patients (10 to 20 years old) with atopy were treated with the cream of Preparation Example 2 for the atopic skin area and the cream of Comparative Example 1 for 2 times per day. Day. Atopic dermatitis severity, IgE change, coral leucocyte (Eosinophil) change, moisture content and skin acidity were measured before and 4 weeks after product application (0 week). There were no adverse events in all subjects participating in the 4-week study.

Table 8 below compares the effect of improving the atopy of an experimenter using the cream prepared in Preparation Example 2 and Comparative Example 1.

Name of sample Atopic dermatitis severity IgE change Coral leukocyte change Moisture content Skin acidity I'm after I'm after I'm after I'm after I'm after Production Example 2 37.48 29.19 434.3 318.4 312 246 25.87 26.88 5.721 6.071 Comparative Example 1 37.77 33.06 446.5 366.5 321 281 26.31 25.03 5.714 5.829

n = 20, p < 0.05

As shown in Table 8, as shown in Table 8, atopic dermatitis severity, atopic dermatitis severity, blood IgE and coral leukocyte changes, water content, and skin acidity, which are criteria for atopic skin, The inventors of the present invention found that the animal mucus extract of the present invention is excellent in atopy improving effect.

[ Manufacturing example  3: An extract containing animal mucus extract Skin moisturizing Cosmetics  Preparation of the composition]

The present Preparation Example 3 was intended to prepare a skin moisturizing cosmetic composition using the mucilage extract obtained from Example 3 above.

To prepare a skin moisturizing cosmetic composition containing an animal's mucus extract, the (B) phase recorded in the following Table 9 was heated and stored at 70 DEG C, and then the (A) phase was added to the (B) Then, the mixture was uniformly emulsified with a homomixer, and then gradually cooled to prepare a cosmetic composition containing creamy animal milky mucilage extract. In addition, general cosmetic compositions containing no animal-derived mucus extract were prepared with compositions as shown in Table 9 below, and were referred to as Comparative Examples 2 to 5, respectively.

Raw material Production Example 3 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 end Stearyl alcohol 8 8 8 8 8 Stearic acid 2 2 2 2 2 Stearic acid cholesterol 2 2 2 2 2 Squalane 4 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 6 Polyoxyethylene (25 mole addition)
Alcohol ester 3 3 3 3 3 Glyceryl monostearate
Aster 2 2 2 2 2 I Example 3
(Frog mucus extract) 5 - - - - glycerin - - 5 - - Na-hyaluronate - - - 5 - 1,3-butylene glycol - - - - 5 Purified water Balance Balance Balance Balance Balance

Unit: wt%

[ Experimental Example  10: skin moisturizing effect measurement experiment]

This experiment was carried out as follows to examine skin moisturizing effect of a cosmetic composition containing an animal mucus extract.

Twenty subjects of the experiment were subjected to the preparation example 3 and the comparative examples 2 to 5 to the lower part. After about 90 minutes, the humidifying ability before and after the application was evaluated by a moisture meter (CM820) Respectively.

division Production Example 3 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Type of moisturizer Frog mucus extract - glycerin Na-hyaluronate 1,3-butylene glycol Moisturizing effect
(△ Hydration) 17 5 10 13 9

As shown in Table 10, it was found that the moisturizing effect was excellent in the facial skin of an experimenter who applied the cream of Preparation Example 3 containing the above-mentioned frog louse mucus extract.

[ Experimental Example  11: Percutaneous  Water loss ( TEWL  : Trans - epidermal water loss ) Measure]

This experiment was carried out using a Tewameter (TM300, Courage and Khazaka Electronic Co., Ltd.) to investigate the trans-epidemic water loss (TEWL) of the cosmetic composition prepared in Preparation Example 3 and Comparative Examples 2 to 5. , Germany) as follows.

Each sample was applied to the right and left faces of 40 women in their 20s and 40s. The samples were divided into 1 hour, 2 hours, 4 hours, and 6 hours of transdermal water The loss was measured three times using a Tewameter (TM300, Courage and Khazaka Electronic Co., Germany) and the average value was used to evaluate the amount of percutaneous water loss. Table 11 below compares the measured transdermal water loss of an experimenter using the cream prepared in Preparation Example 3 and Comparative Examples 2 to 5.

division 1 hours 2 hours 4 hours 6 hours Production Example 3 14.9 12.4 9.9 7.6 Comparative Example 2 18.6 16.7 13.5 10.8 Comparative Example 3 16.8 14.1 12.2 9.5 Comparative Example 4 15.4 14.6 11.9 9.7 Comparative Example 5 15.5 13.8 11.4 9.2

Unit: g / h · m ²

As a result of measurement of transdermal water loss, it was found that the amount of transdermal water loss was reduced in the facial skin of an experimenter who applied the cream of Preparation Example 3 containing the frog mucus extract as shown in Table 11 above. This shows that the skin barrier, including the lipid component of the keratinocyte layer and the epidermal lipid, is improved, thereby reducing the transdermal water loss.

Claims (9)

A mucilage extract of animal eggs selected from snail eggs, coagulant eggs or frog eggs. The method according to claim 1,
Wherein the mucus extract is obtained by crushing and then extracting the animal eggs. The method according to claim 1,
Wherein the mucus extract is contained in an amount of 0.001 to 30% by weight based on the total weight of the composition. 4. The method according to any one of claims 1 to 3,
Wherein the cosmetic composition is an antioxidant composition. 4. The method according to any one of claims 1 to 3,
The cosmetic composition is a cosmetic composition for improving skin wrinkles. 4. The method according to any one of claims 1 to 3,
The cosmetic composition is a cosmetic composition for skin moisturizing. 4. The method according to any one of claims 1 to 3,
The cosmetic composition is for improving the skin barrier. 4. The method according to any one of claims 1 to 3,
Wherein the cosmetic composition is for improving atopy. 4. The method according to any one of claims 1 to 3,
The cosmetic composition is an anti-inflammatory composition.