JPH09263526A - Cosmetic composition and medicinal composition containing extract of a plant in selaginella - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cosmetic composition and a medicinal composition which has anti-inflammatory, antiallergic and antioxidant actions and is excellent in safety. SOLUTION: An extract of a plant (Selaginella moellendorfii Hieron) in Selaginella or one or more kinds of its fractionated products are used as active ingredients in an amount of 0.001-10wt.%. This active ingredient is properly formulated to conventional additives to prepare tooth paste, ointment, lotion, powder hair tonic, shampoo, rinse, treatment, mousse, juice, candy, tablets and the like. The extract is prepared by extracting raw or dried roots. leaves stems of the plant with an organic solvent such as ethanol. The extract is further partitioned with organic solvents and fractionated and isolated through chromatography and the like.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention comprises an organic solvent extract of the genus Selaginella genus Selaginella, which has excellent safety and anti-inflammatory, anti-allergic and antioxidant effects, or a fraction thereof. The present invention relates to a cosmetic composition and a pharmaceutical composition.

[0002]

2. Description of the Related Art The skin is irritated by various external environmental factors such as ultraviolet rays, cold wind and dryness, and is in a state of being easily inflamed. However, anti-inflammatory agents that can be used in cosmetics are limited to substances with weak action such as glycyrrhizinic acid and allantoin, and in reality, satisfactory effects have not been obtained.

Recently, allergic diseases such as hay fever and atopic dermatitis tend to increase. In the allergic reaction, IgE antibody binds to the surface of mast cells via a receptor and causes an antigen-antibody reaction by the invasion of antigen.
It is classified into type I to type IV allergic reactions, including type 1 allergy, but in reality, several of these reactions are combined to cause human lesions. Therefore, it is still a sufficiently effective antiallergic agent. Has not been found.

Since the skin is directly stimulated by external environmental factors such as ultraviolet rays, active oxygen is particularly easily generated. It is said that the active oxygen generated in the living body is usually eliminated by enzymes such as catalase and superoxide dismutase. However, in vivo,
If such action is insufficient or active oxygen is excessively present, various tissue disorders such as lipid peroxide production, stains and wrinkles are likely to occur.

Antioxidants for preventing and treating these disorders include synthetic products such as dibutylhydroxytoluene and butylhydroxyanisole, and natural product-based products such as tocopherol and rosmarinic acid. Although the effect is strong, its use is limited due to its safety to the living body, and it is said that natural products are safer than synthetic products. There is a problem that it is inferior to synthetic products in terms of stability and effect.

[0006]

DISCLOSURE OF THE INVENTION The object of the present invention is to contain an active ingredient of natural product type which exhibits anti-inflammatory, anti-allergic and anti-oxidant effects and is safe from the viewpoint of living body. It is to provide a cosmetic composition and a pharmaceutical composition.

[0007]

Means for Solving the Problems In view of such a situation, the present inventors have searched for natural products, and as a result, it was found that the organic solvent extract and the fraction thereof of dog caterpillar have an excellent anti-inflammatory action. The inventors have found that they have an antiallergic action and an antioxidant action, and have completed the present invention.

[0008] That is, the present invention provides a cosmetic composition containing an organic solvent extract of canine caterpillar of the genus Selaginella of the genus Selaginaceae or a fraction thereof. The present invention also provides a pharmaceutical composition containing an organic solvent extract of dog squirrels of the genus Selaginella of the genus Selaginaceae or a fraction thereof. Such a composition is an anti-inflammatory agent,
It is useful as an anti-allergic agent or antioxidant.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION
laginella moellendorfii Hieron (Chinese name: Gangnammaki) is a plant belonging to the genus Seraginella (Selaginella) and is distributed in most of the southern region in China, and is known as the ground bark or ground branch. , It is mainly used as a traditional Chinese medicine with hemostatic effect for bleeding, jaundice, and child attraction.

[0012] The organic solvent extract of canine caterpillar of the present invention can be obtained by extracting raw or dried plant bodies with an organic solvent. As the plant body, any of a root portion, a leaf portion and a stem portion may be used. The extraction conditions are not particularly limited, but for example, dried dogs and cats (sliced or crushed) 1
1 to 30 parts of organic solvent is added to 1 part, and room temperature to 90 ° C.
Over a temperature range of about 1 with or without stirring.
Extraction is carried out for a period of time to 1 week, the extract is filtered or centrifuged to remove solids, and then the organic solvent is distilled off by a suitable method known per se.

The extraction solvent used in the present invention includes, for example, benzene, diethyl ether, methylene chloride, ethyl acetate, butyl acetate, acetone, methanol, ethanol, butanol, 1,3-butylene glycol and water-containing ethanol, water-containing methanol, water-containing. Solvents selected from butanol, hydrous acetone and hydrous dimethylformamide may be mentioned, and these may be used alone or in admixture of two or more. Preferred solvents include methanol, ethanol,
Acetone, and water-containing solvents and mixed solvents thereof are included.

[0012] The fraction of the organic solvent extract of Acacia nigra of the present invention is a conventional separation and fractionation method such as liquid-liquid partitioning using the above-mentioned organic solvent extract using various solvents, chromatography and the like. It is obtained by fractionating with.

These organic solvent extracts of dogtooth squirrel and fractions thereof may be used alone or in combination of two or more kinds,
The total amount is preferably 0.001 to 10% by weight. If it is less than 0.001% by weight, the anti-inflammatory effect, antiallergic effect and antioxidant effect cannot be obtained, while if it exceeds 10% by weight, skin irritation becomes high.

The composition of the present invention can be in the form of any oral preparation, external preparation or internal preparation, which is combined with other desired active ingredients by a method known per se, and toothpaste, ointment, lotion,
It can be formulated into cosmetic oils, creams, emulsions, packs, powders, hair tonics, shampoos, rinses, treatments, mousses, juices, candy, tablets and the like. It should be noted that the organic solvent extract and the fractionated product of Acacia nigra which are added to the composition of the present invention are derived from natural products and no toxicity is observed.

The composition of the present invention is applied to skin, scalp, hair, buccal cavity and oral administration.

Hereinafter, the present invention will be described in more detail based on test examples and examples. However, the present invention is not limited to the following examples and may be appropriately modified within the scope of the invention. It can be implemented.

[0017]

【Example】

(1) Preparation of an organic solvent extract of dog caterpillar and a fraction thereof (Reference Example 1) Organic solvent extract 815 g of dried dog caterpillar was appropriately cut and extracted with 13 liters of ethanol under reflux three times (2 h). The combined solution of the three extracts was concentrated under reduced pressure and then dried to obtain 19.1 g of an organic solvent extract. (Yield 2.34%)

(Reference example 2) Fractionated product To 19.1 g of the extract obtained in (Reference example 1) was added 1 liter (x3) of 50% water-containing ethanol, and liquid-liquid was added with 1 liter of hexane (x3). The mixture was distributed, the aqueous ethanol layers were collected and concentrated under reduced pressure to obtain 9.15 g of a fraction of the organic solvent extract.

Reference Example 3 Organic Solvent Extract 400 g of dried dog-toothed caterpillar was appropriately cut and subjected to reflux extraction with 7 liters of chloroform three times (2 h). The combined solution of the three extracts was concentrated under reduced pressure and then dried to obtain an organic solvent extract.

(2) Efficacy Test Example 1 Anti-Inflammatory Action In order to investigate the anti-inflammatory action of the organic solvent extract and its fractions prepared above, a test was carried out using white guinea pigs to suppress ultraviolet erythema.

UV erythema inhibition test using white guinea pigs: Hartley white guinea pigs (female, 400-60)
0 g) was shaved on the back, and after fixing, the back was divided into 4 compartments (2.5 cm × 2.5 cm), and the fraction of Reference Example 2 was diluted with a diluting solvent (ethanol: propylene glycol = 3: 7) to 5
The test sample diluted to 0.1% was applied to each section in an amount of 0.1 ml. After 30 minutes, UV UVB irradiation of 3.0 J / cm ²
Immediately after that, 0.1 ml of the test sample was applied again to each part,
After 3 hours, 24 hours, and 48 hours, a value of each part was measured using a color difference meter. As a control, the same operation was performed using the diluted solvent of the test sample. The difference between the a value after UV irradiation and the a value before UV irradiation is defined as the delta a value,
The ratio with the delta a value of the control group was calculated as the inhibition rate. Table 1 shows the results.

[0022]

[Table 1]

From Table 1, it can be seen that the organic solvent extract of catnipweed, which is the active ingredient in the composition of the present invention, has an anti-inflammatory effect.

(3) Efficacy Test Example 2 Antiallergic Action Histamine Release Inhibitory Action In order to investigate the antiallergic action, a test was carried out to inhibit histamine release by compound 48/80 from rat abdominal mast cells. The type I allergic reaction is caused by the release of chemical mediators such as histamine and leukotriene (degranulation) as a result of the antigen-antibody reaction on the surface of mast cells. Therefore, the measurement was performed by using the inhibitory action of histamine, which is one of the chemical mediators released from mast cells when an inflammatory agent (compound 48/80) was administered, as an index.

Histamine release inhibition test: rat (Wistar
/ ST system, body weight about 250g) and 0.1% BS in the abdominal cavity
A phosphate buffer was injected, and mast cells were separated according to a conventional method to prepare a cell suspension. The extracts obtained from Reference Examples 1 and 3 were added to this suspension (2 ml), and 10 minutes later, a inflammatory agent (compound 48/80) was added, and 10 minutes later, the amount of free and intracellular histamine was measured. It was quantified by the fluorescence method. As a control, physiological saline was added instead of the test sample.
The release inhibition rate (%) was calculated by the following formula.

[0026]

[Equation 1]

The test results are shown in FIG. From FIG. 1, it can be seen that the organic solvent extract of dog caterpillar, which is the active ingredient in the composition of the present invention, suppresses the release of histamine.

(4) Efficacy test example 3 Antioxidant activity In order to investigate the antioxidant activity, an SOD activity measurement test by the NBT method was carried out. The measurement was carried out by adding azo blue nitroblue tetrazolium (NBT) to a test sample solution in which superoxide radicals were generated by xanthine-xanthine oxidase, and measuring the absorbance.
The principle is to measure the trapped amount of superoxide radicals (SOD activity) by the change in absorbance.

SOD activity test: 0.05 M Na ₂ CO ₃
Buffer (pH 10.2) 2.4 ml, 3 mM xanthine solution 0.1 ml, 3 mM EDTA aqueous solution 0.1 ml, 0.1
5 wt% bovine serum albumin aqueous solution 0.1 ml, 0.75 m
0.1 ml of M NBT aqueous solution and 0.1 ml of each of the extract solutions obtained from Reference Examples 1 and 2 were used to obtain a final concentration of 0.025 m.
g / ml, 0.05 mg / ml, and 0.1 mg / ml were mixed, and the mixture was allowed to stand at 25 ° C. for 10 minutes, and then the absorbance in a blank test was adjusted to 0.20 to 0.23. Add 0.1 ml of xanthine oxidase (Sigma) to 2
Incubated at 5 ° C for 20 minutes. Then 6 mM
Add 0.1 ml of CuCl ₂ solution to stop the enzyme reaction,
Absorbance at 560 nm was measured (A).

As a control, the same operation was carried out by using a diluting solvent instead of the sample solution (B). As a blank for each sample, after adding a test sample solution, 6 mM CuCl ₂ was added.
0.1 ml of the solution was added, and 0.1 ml of xanthine oxidase was added in the same manner, and the absorbance was measured (C). The inhibition rate (%) was calculated by the following formula.

[0031]

[Equation 2] A: Absorbance of sample by enzyme reaction B: Absorbance of control by enzyme reaction C: Absorbance of sample by non-enzyme reaction

The test results are shown in FIG. From FIG. 2, it can be seen that the organic solvent extract of Kata-Hiba, which is an active ingredient in the composition of the present invention, exhibits a SOD activity inhibitory action, and thus an antioxidant action, in a concentration-dependent manner.

(5) Example (Example 1) Toothpaste component Blending amount (wt%) Organic solvent extract obtained in Reference Example 1.0 Calcium carbonate 45.0 Sodium carboxymethyl cellulose 1.0 Glycerin 20.0 Sodium lauryl sulfate 1.5 Sodium saccharin 0.1 Perfume 1.0 Purified water Remainder 100.0 Total Toothpaste was prepared by a conventional method according to the above formulation.

(Example 2) Toothpaste component Compounding amount (% by weight) Organic solvent extract obtained in Reference Example 1.0 Dibasic calcium phosphate 45.0 Sodium carboxymethyl cellulose 0.5 Glycerin 20.0 Sodium lauryl sulfate 1 .5 Saccharin sodium 0.1 Fragrance 0.5 Purified water Remainder 100.0 Total Toothpaste was prepared by a conventional method according to the above formulation.

Example 3 Powdered Toothpaste Component Blending Amount (% by Weight) Organic Solvent Extract Obtained in Reference Example 2.5 Dicalcium Phosphate 55.0 Calcium Carbonate 30.0 Sorbitol 10.0 N-Lauroyl Sarcosine Sodium 0.5 Sucrose fatty acid ester 1.0 Saccharin sodium 0.1 Fragrance 0.5 Purified water balance Total 100.0 According to the above-mentioned prescription, a toothpaste was prepared by a conventional method.

Example 4 Mouthwash Ingredients Amount (wt%) Organic solvent extract obtained in Reference Example 1.5 Ethanol 10.0 Saccharin sodium 0.2 Polyoxyethylene hydrogenated castor oil 2.0 Glycerin 5. 0 Fragrance 1.0 Purified water balance Total 100.0 According to the above formulation, a mouthwash was prepared by a conventional method.

Example 5 Ingredient for Gel Oral Agent Component Amount (wt%) Organic Solvent Extract Obtained in Reference Example 5.0 Saccharin Sodium 1.0 Perfume 0.1 Polyethylene Glycol 400 50.5 Polyethylene Glycol 4000 43.4 Total 100.0 According to the above formulation, a gel oral preparation was prepared by a conventional method.

Example 6 Ointment Ingredients Amount (% by Weight) Fraction of Organic Solvent Extract Obtained in Reference Example 1.0 Propylene Glycol # 400 15.0 Macrogol Ointment 84.0 Total 100.0 An ointment was prepared by a conventional method according to the above formulation.

(Example 7) Lotion component Blending amount (wt%) Fraction of organic solvent extract obtained in Reference Example 0.5 Glycerin 6.0 Ethanol 8.0 Polyexethylene hydrogenated castor oil 0.8 Methylparaben 0.05 Citric acid 0.05 Sodium citrate 0.07 Fragrance 0.1 Purified water balance Total 100.0 A lotion was prepared by a conventional method according to the above formulation.

Example 8 Cosmetic Oil Ingredients Amount (% by Weight) Fraction of Organic Solvent Extract Obtained in Reference Example 2.0 Cholesteryl Stearate 1.0 Olive Oil 2.0 Squalane Residual Total 100 0.0 A cosmetic oil was prepared by a conventional method according to the above formulation.

Example 9 Cream Component Blending Amount (wt%) Component (A) Fraction of Organic Solvent Extract Obtained in Reference Example 2.0 Sarah Beeswax 4.0 Cetanol 2.0 Stearic Acid 1. 0 Isopropyl myristate 5.0 Lanolin 2.0 Liquid paraffin 9.0 Self-emulsifying glyceryl monostearate 3.0 Polyoxyethylene sorbitan monostearate 1.5 Component (B) Methyl paraoxybenzoate 0.1 Propylene glycol 5 0.0 Fragrance 0.2 Purified water balance Total 100.0 According to the above formulation, a cream was prepared by a conventional method.

(Example 10) Emulsion component Blending amount (wt%) Component (A) Fraction of organic solvent extract obtained in Reference Example 0.2 Stearyl glycyrrhetinate 0.1 Liquid paraffin 5.0 Vaseline 2 0.0 Beeswax 1.0 Sorbitan sesquioleate 2.0 Component (B) Polyethylethylene oleyl ether 2.5 Ethyl paraoxybenzoate 0.1 Propylene glycol 5.0 Carboxyvinyl polymer 0.5 Potassium hydroxide 0.5 Perfume 0.2 Purified water balance Total 100.0 According to the above formulation, an emulsion was prepared by a conventional method.

Example 11 Pack Component Blending Amount (% by Weight) Fraction of Organic Solvent Extract Obtained in Reference Example 5.0 Vinyl Acetate / Styrene Copolymer 10.0 Polyvinyl Alcohol 10.0 Solbit 5 0.0 allantoin 0.5 kaolin 7.0 ethanol 5.0 fragrance 2.0 ethyl paraoxybenzoate 0.1 purified water balance 100.0 According to the above-mentioned prescription, a pack was prepared by a conventional method.

(Example 12) Powder component Blending amount (wt%) Fraction of the organic solvent extract obtained in Reference Example 1.0 Dextrin 96.0 Talc 2.0 Decaglyceryl stearate 1.0 Total 100 0.0 Powder was prepared by a conventional method according to the above formulation.

Example 13 Chewing Gum Ingredients Amount (wt%) Organic Solvent Extract Obtained in Reference Example 10.0 Gum Base 65.0 Mannitol 20.0 Sorbit 3.5 Perfume 1.5 Total 100.0 A chewing gum was prepared by a conventional method according to the above formulation.

(Example 14) Gargling tablet Ingredient blending amount (wt%) Organic solvent extract obtained in Reference Example 5.0 Sodium hydrogencarbonate 55.0 Sodium diphosphate 15.0 Polyethylene glycol 6000 3. 0 Citric acid 12.0 Ethanol 6.0 Fragrance 4.0 Total 100.0 According to the above-mentioned prescription, a mouthwash tablet was prepared by a conventional method.

(Example 15) Lozenge Component Blending amount (wt%) Organic solvent extract obtained in Reference Example 0.001 Gum arabic 6.0 Fructose 20.0 Glucose 20.0 Maltose 30.0 Perfume 0.1 Purified water Residual total 100.0 According to the above formulation, a troche was prepared by a conventional method.

Example 16 Hair Tonic Ingredients Amount (wt%) Organic Solvent Extract Obtained in Reference Example 0.1 Ethanol 70.0 Tocopherol Acetate 0.05 Glycyrrhizic Acid 0.1 Propylene Glycol 3.0 l -Menthol 0.1 Fragrance / Dye Appropriate Fragrance Solubilizer Appropriate Purified Water Remainder Total 100.0 According to the above formulation, a hair tonic was prepared by a conventional method.

[0049]

EFFECTS OF THE INVENTION A cosmetic composition and a pharmaceutical composition comprising the organic solvent extract of dogtooth catfish or the fraction thereof of the present invention have anti-inflammatory effects such as ultraviolet erythema inhibition, anti-allergic effects, and the like in the body. It is excellent in antioxidant action such as suppression of peroxide generation and has no problem in safety, and can be used as an oral preparation, an external preparation and an internal preparation.

[Brief description of drawings]

FIG. 1 is a graph showing the histamine release inhibitory effect (anti-allergic effect) of an organic solvent extract of dog caterpillar.

FIG. 2 is a graph showing the superoxide radical activity inhibitory effect (antioxidant effect) of the organic solvent extract of dog caterpillar.

Continuation of the front page (51) Int.Cl. ⁶ Identification number Office reference number FI Technical display location A61K 7/26 A61K 7/26 9/20 9/20 U 35/78 ABE 35/78 ABEB ABF ABFB AED AEDB

Claims (7)

[Claims] 1. A cosmetic composition comprising an organic solvent extract of dog caterpillar of the genus Selaginella of the genus Selaginaceae or a fraction thereof. 2. The organic solvent for extraction is methanol, ethanol, butanol, acetone, ether, ethyl acetate,
The cosmetic composition according to claim 1, which is dimethylformamide, chloroform, or a mixed solvent thereof. 3. A pharmaceutical composition containing an organic solvent extract of dog caterpillar of the genus Seraginella of the genus Selaginaceae or a fraction thereof. 4. The organic solvent for extraction is methanol, ethanol, butanol, acetone, ether, ethyl acetate,
The pharmaceutical composition according to claim 3, which is dimethylformamide or chloroform, or a mixed solvent thereof. 5. The use according to any one of claims 1 to 4, which is used as an anti-inflammatory agent. 6. Use as an anti-allergic agent
4. Use according to any one of 4 above. 7. The use according to any one of claims 1 to 4, which is used as an antioxidant.