KR20150088461A - Insecticidal composition for comprising extract and mixture from Cinnamomum cassia, Schizandra chinensis and Syzygium aromaticum - Google Patents

Abstract

The present invention relates to an insecticidal composition comprising a Cinnamomum cassia extract, a Schisandra chinensis extract, and a Syzygium aromaticum extract. According to the present invention, an insecticidal composition has insecticidal effects in Tetranychus urticae, Aphis gossypii, Spodoptera exigua, Trialeurodes vaporariorum, Frankliniella occidentalis, and Meloidogyne incognita, and can be used as an eco-friendly material of insect damage using the same.

Description

[0001] The present invention relates to an insecticidal composition comprising Cinnamomum cassia, Schizandra chinensis and Syzygium aromaticum,

The present invention relates to an insecticidal composition containing cinnamon extract, omija extract and clove extract or a mixture thereof, and more particularly to an insecticidal composition comprising an extract obtained by using ethanol as a solvent and a mixture thereof.

Chemical synthetic pesticides have been used to increase food resources by controlling agricultural pests. However, ecosystem destruction due to abuse of chemical synthetic pesticides and the emergence of resistant pests and drastic decrease of useful insects were caused. As a side effect of these chemical synthetic pesticides, it is required to develop biocidal pesticides having low toxicity, high efficiency and insecticidal power.

Biological pesticides include natural products, host-resistant microorganisms, pathogens isolated from natural environments, and natural enemies. Commercialized products include microbes, pheromone-containing biochemical pesticides, plant pesticides, natural enemies and others.

Among them, plant pesticides show insecticidal and repellent effects against pests by using useful materials derived from plants. They can be developed at low cost compared to the development of chemical synthetic pesticides, which have no side effects due to the use of natural materials and require huge time and cost investment. Do.

One of the most well known pesticides in plant pesticides is the pyrethroid compound of the insect pest, and many synthetic similar products have been developed and marketed. Nicotine, a major component of cigarettes, and triterpenoid azadirachtin, a major component of mull beads, are also used as natural insecticides. Other compounds such as flavonoids, saponins, phenols, and alkaloids have also been reported to exhibit insecticidal activity.

Cinnamomum cassia belongs to the genus Lauraceae and is known to be distributed in India, China, Sri Lanka, Vietnam and Australia. Cinnamic acid, cinnamaldehyde, diterpenoid, cinnamic acid, and polyphenols have been reported as active ingredients of cinnamon. Antimicrobial activity and various physiological activities of these substances and their derivatives have been reported.

Schizandra chinensis belongs to the genus Omiza and is a medicinal plant that uses red fruit as medicines and food ingredients. The ingredients contained in the fruit of Omija contain about 1.5% of lignan compounds such as schizandrin, gomisin A and gomisin N. Especially, schizandrin has high anticancer activity, immunoregulatory activity and antimicrobial activity. Hanba also report the lignan component such as gomisin A gomisin N and that this is a tiny-creatures-killing effect. (Mitsuo M., K. Hirotaa, M. Fukuyamaa, Y. Ishikawab and H. Kameokaa. [1997] Natural product letters 12 (3 ) 175-180.)

Cloze ( Syzygium aromaticum ) is a tropical evergreen schistose, and when the bud turns from green to red, it is harvested and dried. It has a characteristic odor, strong taste, and is known to paralyze the tongue. It is reported that 16 ~ 19% of essential oil is contained in the bud, and the major components are eugenol, caryophyllene, methyl-N-amylketone, methyl salicylate and benzylalcohol.

Accordingly, the inventors of the present invention have completed the present invention by confirming that the insecticidal activity is revealed by using cinnamon, omija, clove extract and mixtures thereof in order to develop an insecticide utilizing natural products and it is applicable as an environmentally friendly pesticide.

An extract of Omija which maximizes the content of shuzendrine which is an active ingredient of Omija, a method of extracting it, and a soft capsule containing the Omija extract as an active ingredient (Application 10-2002-0085632) Plants and insecticidal compounds having insecticidal activity (Application 10-2001-0026179) (Registered trademark 10-0941915) containing cinnamic aldehyde, which is separated and purified from cinnamon, and a substance structurally similar thereto, A natural insecticidal composition comprising a Chinese Rhodiola extract and a clove extract as an active ingredient (Registration No. 10-1221735)

The present inventors intend to provide a natural insecticidal composition comprising cinnamon, omija, clove extract and a mixture thereof.

In order to accomplish the above object, the present invention provides a composition for natural insecticide comprising cinnamon, omija, clove extract and a mixture thereof.

The extract of the present invention may be prepared by various methods for producing plant extracts known in the art. For example, shaking extraction method, low temperature extraction method, high temperature extraction method or reflux extraction method may be used, but the present invention is not limited thereto.

The cinnamon, omija or clove extract may be water or an extract extracted with an organic solvent.

Examples of the organic solvent include hexane, chloroform, ethyl acetate, butanol, methanol, propanol, ethanol, and mixtures thereof.

When the extraction solvent is water, it may be subjected to hot water extraction, and in the case where the extraction solvent is ethanol, reflux extraction may be used.

Further, the extract extracted with the extraction solvent may be further subjected to a fractionation process with a solvent such as hexane, chloroform, ethyl acetate, butanol, etc., and at least one solvent may be used in the fractionation.

The temperature for preparing the extract may be 25 ° C to 100 ° C, but is not limited thereto, and the extraction time may be 60 minutes to 360 minutes, but is not limited thereto. The extraction process may be performed using a shaking extractor, an ultrasonic extractor, or a fractionator, but is not limited thereto. The extract thus prepared may be subjected to vacuum filtration or drying to remove the solvent, followed by both reduced pressure filtration and drying. The reduced pressure filtration can be performed by a low-temperature decompression concentration method using a reduced pressure concentrator, But it is not limited thereto.

According to a specific embodiment of the present invention, the extract is obtained by refluxing ethanol by adding ethanol to the plant, filtering the resulting extract and concentrating the extract.

According to one embodiment of the present invention, the cinnamon extract, Omija extract or clove extract may be an organic solvent extract fraction. For example, it may comprise a hexane fraction of the cinnamon extract. It may also comprise a hexane fraction of the above Omija extract. And the ethyl acetate fraction of the clove extract.

According to one embodiment of the present invention, the composition for insecticidal use may be a mixture of the extract of Omija and the extract of clove at the same concentration in the volume ratio of 1: 1: 1 to 1: 2: 1. When the insecticidal composition contains the same amount of cinnamon extract at a ratio of 1: 1: 1 to 1: 2: 1 by volume, the cinnamon extract, the omija extract or the clove extract, You can have more.

The insecticidal composition has an insecticidal effect. For example, spotted mites, effective for (Tetranychus urticae), cotton aphid (Aphis gossypii), greenhouse whitefly (Trialeurodes vaporariorum), beet armyworm (Spodoptera exigua), flower thrips (Frankliniella occidentalis), root-knot nematode (Meloidogyne sp.) . ≪ / RTI >

The composition for insecticidal use may further comprise a substance to be mixed with a conventional insecticide, and it is preferable that the insecticidal composition is controlled according to the insecticide formulation, the application site, and the application method.

The composition for insecticidal use may be treated on its own by itself or may be prepared in a flowable form. Suspensions, microsuspension, emulsions, powders, granular concentrates, microemulsions and the like.

The insecticidal composition according to the present invention has an insecticidal effect and can be used as an environmentally friendly natural pesticide preparation having an insecticidal effect especially on the spotted mite, cotton aphid, greenhouse powdery mildew, have.

1 is a block diagram for explaining a fractionation process of a solvent from a cinnamon extract.
FIG. 2 is a block diagram for explaining the fractionation process of the solvent from the Schizandra chinensis extract. FIG.
FIG. 3 is a block diagram for explaining the fractionation process by the solvent from the clove extract. FIG.

EXAMPLES Hereinafter, the present invention will be described in detail with reference to Examples. However, the following Examples are illustrative of the present invention, and the present invention is not limited to the following Examples.

<Examples>

1. Extract preparation and fractionation

end. Sample preparation

Cinnamomum cassia , Schizandra chinensis and Syzygium aromaticum were pulverized and 70% ethanol was added and the mixture was refluxed at 80 ° C for 3 hours. The impurities were removed using a filter paper, and the filtrate was concentrated under reduced pressure to dryness A weight of 100 g was obtained.

I. Fractionation of the extract

100 g of each extract was dissolved in distilled water, and the fractions were sequentially fractionated using hexane, chloroform, ethyl acetate and butanol in hexane-soluble, chloroform-soluble, ethyl acetate-soluble, butanol-soluble and water-soluble fractions.

The cinnamon extract was fractionated to obtain 12.9 g of hexane solubility, 30 g of chloroform solubility, 2.9 g of ethyl acetate solubility, 14.7 g of butanol availability and 32 g of water solubility.

The omija extract was fractionated to obtain 30 g of hexane solubility, 18 g of chloroform solubility, 6 g of ethyl acetate solubility, 10 g of butanol solubility and 27 g of water solubility.

The clove extract was fractionated to obtain 25 g of hexane solubility, 11 g of chloroform solubility, 33 g of ethyl acetate solubility, 17 g of butanol availability and 12 g of water solubility

All. Mixing of Extracts

 The above cinnamon extract, Omiza extract and Clove extract were respectively prepared at 5,000 ppm. Then, the cinnamon extract and the omija extract prepared at 5,000 ppm were mixed at a ratio of 1: 1 (v / v) or the Omiza extract and the clove extract at a ratio of 1: 1 (v / v).

In another example, cinnamon extract, omija extract and clove extract prepared at 5,000 ppm were mixed at a ratio of 1: 1: 1 (v / v) at a ratio of 2: 1: 1: 2.

Experimental Example 1. Insecticidal Effect Test of Cinnamon, Omiza and Clove Extracts

end. Sample preparation

Cinnamon, omija and clove were refluxed for 4 hours at 80 ° C with 70% ethanol, concentrated using a vacuum concentrator, and used as a sample. The sample concentration was adjusted to 63 to 1,000 ppm to determine the insecticidal effect Respectively.

I. Examination of insecticidal effect by test and leaf method

The Tetranychus urticae used in the experiment was used for the test under the condition of room temperature of 28 ℃ and relative humidity of 40%. The petri dish with a diameter of 6cm was put on a cotton pad and moistened with distilled water to provide water. Each sample was prepared for each concentration and then immersed in a leaf section (3.5 cm) for 1 minute and shaved for 1 hour with the leaf section facing upward. The shredded leaves were placed in the center of petri-dish, and then 30 seeds of spotted mites were inoculated. After spraying, the plants were kept in an incubator at 25 ℃ for 48 hours.

Cotton aphid (Aphis gossypii), greenhouse whitefly (Trialeurodes vaporariorum), beet armyworm (Spodoptera exigua), and flower thrips (Frankliniella occidentalis) were to be supplied with water-wet laying the cotton wool to 6cm of the petri-dish diameter chokchokhi distilled water. Each sample was prepared for each concentration, dipped in leaf sections (4.5 cm) for 1 minute, and shaved for 1 hour with the leaf sections facing up. The shade cuts were placed in the center of a petri dish and then 10 inoculated with cotton aphid and greenhouse powder were sprayed. After 48 hours, the mortality was examined 48 hours after storage in a 25 ° C incubator.

Meloidogyne sp. Was sterilized at 121 ℃ for 15 min. After drying in a hood, tomato seedlings were planted, 1,000 root-knot nematodes were inoculated, and cultivated at 25 ℃ for 15 days. After 3 days of flowering, the number of root - knot nematode was checked and the insecticidal rate was examined.

All. result

1) Insecticidal effect of cinnamon extract

Table 1 shows the insecticidal effect of each concentration of cinnamon extract. At the concentration of cinnamon extract of 1,000ppm, the insecticidal effect of spotted mite was 72.5%. In addition, the insecticidal effect of cotton aphid was 63.8% at the cinnamon extract concentration of 1,000 ppm.

Sample concentration
(ppm) Insecticidal rate (%) Spotted mite Cotton aphid Chestnut Greenhouse powder Thrush Root-knot nematode 1,000 72.5 63.8 51.4 59.7 49.0 35.9 500 55.7 51.3 43.8 59.7 48.0 49.3 250 38.2 44.1 32.5 38.2 45.7 35.9 125 15.5 9.6 5.1 15.9 8.4 0 63 0 0 0 0 0 0

2) Insecticidal effect of Omiza extract

The insecticidal effect of Omiza extract was as shown in Table 2. The insecticidal effect of greenhouse powder was 43.2% at 1,000 ppm of Omiza extract. The insecticidal effect of spotted mite was 35.8% at the concentration of 1,000 ppm of Omiza extract.

Sample concentration
(ppm) Insecticidal rate (%) Spotted mite Cotton aphid Chestnut Greenhouse powder Thrush Root-knot nematode 1,000 35.8 21.5 19.7 43.2 17.9 22.0 500 12.8 9.3 8.2 11.4 9.1 10.2 250 4.3 3.6 1.5 4.9 0 0 125 0 0 0 0 0 0 63 0 0 0 0 0 0

3) Insecticidal effect of clove extract

Table 3 shows the insecticidal effects of clove extracts. The insecticidal effect of cotton aphid was 55.7% at the concentration of 1,000 ppm of clove extract. In addition, the insecticidal effect of spotted mite at the concentration of 1,000 ppm of clove extract was 49.5%.

Sample concentration
(ppm) Insecticidal rate (%) Spotted mite Cotton aphid Chestnut Greenhouse powder Thrush Root-knot nematode 1,000 49.5 55.7 42.8 48.9 33.1 25.8 500 31.3 28.5 13.5 30.0 32.4 24.1 250 22.1 20.6 11.6 18.0 11.3 9.2 125 0 0 0 0 0 0 63 0 0 0 0 0 0

Experimental Example 2 Insecticidal Effect According to Mixing Ratios of Cinnamon, Omiza and Clove Extracts

end. Preparation of mixtures according to mixing ratio of two extracts

Cinnamon extract, omija extract and clove extract were respectively prepared at 5,000 ppm. Then, the cinnamon extract and the omija extract prepared at 5,000 ppm were mixed at a ratio of 1: 1 (v / v) or the Omiza extract and the clove extract at a ratio of 1: 1 (v / v).

I. Examination of insecticidal effect by test and leaf method

The Tetranychus urticae used in the experiment was used for the test under the condition of room temperature 28 ℃ and relative humidity 40%. The petri dish with 6cm diameter was put on a cotton pad and moistened with distilled water to supply water. Each sample was prepared for each concentration and then immersed in a leaf section (3.5 cm) for 1 minute and shaved for 1 hour with the leaf section facing upward. The shredded leaves were placed in the center of petri-dish, and then 30 seeds of spotted mites were inoculated. After spraying, the plants were kept in an incubator at 25 ℃ for 48 hours.

Cotton aphid (Aphis gossypii), greenhouse whitefly (Trialeurodes vaporariorum), beet armyworm (Spodoptera exigua), and flower thrips (Frankliniella occidentalis) were to be supplied with water-wet laying the cotton wool to 6cm of the petri-dish diameter chokchokhi distilled water. Each sample was prepared for each concentration, dipped in leaf sections (4.5 cm) for 1 minute, and shaved for 1 hour with the leaf sections facing up. The shade cuts were placed in the center of a petri dish and then 10 inoculated with cotton aphid and greenhouse powder were sprayed. After 48 hours, the mortality was examined 48 hours after storage in a 25 ° C incubator.

Meloidogyne sp. Was sterilized at 121 ℃ for 15 min. After drying in a hood, tomato seedlings were planted, 1,000 root-knot nematodes were inoculated, and cultivated at 25 ℃ for 15 days. After 3 days of flowering, the number of root - knot nematode was checked and the insecticidal rate was examined.

All. Preparation of mixtures according to mixing ratio of three extracts

Cinnamon extract, omija extract and clove extract were respectively prepared at 5,000 ppm. The prepared cinnamon extract, Omiza extract and clove extract were mixed at a ratio of 1: 1: 1 (v / v), 2: 1: 1, 1: 2: 1, .

la. result

1) Insecticidal effect according to mixing ratio of 2 kinds of extracts

Table 4 shows the insecticidal effect of the mixture of cinnamon extract and omija extract mixed at a ratio of 1: 1 (v / v), and the mixture of Omiza extract and clove extract at a ratio of 1: 1.

The mixture of cinnamon and omija extract showed high insecticidal effect of 75.8% of greenhouse powder, and the mixture of omija and clove extract showed insecticidal effect of greenhouse powder 66.6%. This showed a higher insecticidal effect at the mixture than the single extract.

Insecticidal rate (%) Spotted mite Cotton aphid Chestnut Greenhouse powder Thrush Root-knot nematode Cinnamon: Omiza
(1: 1) 69.5 65.1 60.2 75.8 58.5 65.9 Omija: cloves
(1: 1) 61.4 62.1 54.1 66.6 51.0 61.4

2) Insecticidal effect according to mixing ratio of three extracts

The insecticidal effect of the mixture of cinnamon extract, omija extract and clove extract mixed at a mixing ratio of 1: 1: 1 (v / v / v), 2: 1: 1, 1: 2: 1 and 1: Table 5 shows the results.

2: 1: 1 ratio of cinnamon extract, omija extract and clove extract showed the highest insecticidal effect.

Mixing ratio
(v / v / v) Insecticidal rate (%) Spotted mite Cotton aphid Chestnut Greenhouse powder Thrush Root-knot nematode 1: 1: 1 66.2 62.5 58.2 80.2 64.5 68.2 2: 1: 1 72.3 68.8 62.7 86.6 68.1 68.5 1: 2: 1 69.3 65.1 56.8 85.3 62.8 65.3 1: 1: 2 65.2 62.4 58.4 82.1 50.3 62.1

Claims (5)

An insecticidal composition comprising cinnamon, omija and clove extracts characterized by a control against at least one plant insect selected from the group consisting of spotted mite, cotton aphid, parrot moth, greenhouse powder, flower yellow thrips, and root-knot nematode. The insecticidal composition according to claim 1, which comprises a mixture ratio of cinnamon extract, omija extract and clove extract of 2: 1: 1 (v / v / v). The insecticidal composition according to claim 1, which comprises a mixture ratio of cinnamon extract, omija extract and clove extract of 1: 2: 1 (v / v / v). The insecticidal composition according to claim 1, which comprises a mixture ratio of cinnamon extract and omija extract of 1: 1 (v / v). 2. The insecticidal composition according to claim 1, wherein the composition comprises 1: 1 (v / v) of a mixture of Omiza extract and Clove extract.

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