KR20150069775A

KR20150069775A - Cosmetic compositions preventing or relieving the skin aging by menopause

Info

Publication number: KR20150069775A
Application number: KR1020130156300A
Authority: KR
Inventors: 김미선; 조환일; 이상화
Original assignee: 주식회사 엘지생활건강
Priority date: 2013-12-16
Filing date: 2013-12-16
Publication date: 2015-06-24

Abstract

The present invention relates to a cosmetic composition for preventing or improving aging skin including extracts of Belamcanda chinensis, genistein and daizein and, more specifically, a cosmetic composition for preventing or improving aging skin in climacteric period, which has good effects by combining the extracts wherein the cosmetic composition has improved solubility and good effect of improving aging skin.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for preventing or improving skin aging,

The present invention relates to a cosmetic composition capable of preventing or ameliorating skin aging after menopausal period, and relates to a cosmetic composition capable of improving skin aging such as reduction of elasticity and wrinkle due to reduction of female hormone after menopause.

Women become menopausal (menopause) when the female hormone estrogen is reduced. After menopause, women experience various menopausal symptoms such as facial flushing and depression due to a lack of estrogen secretion as ovarian function decreases.

Estrogen has been reported to be a potent anti-aging substance that improves skin aging, as estrogen is applied to the skin to significantly improve elasticity, dryness, and wrinkles. However, these hormone components have limitations that they can not be used as a material for cosmetics. Therefore, there is a need to find estrogen replacement effect ingredients to improve the causes of skin aging in menopause.

Isoflavones, which are mostly contained in soybeans and the like, are phytoestrogens having a structure similar to estrogen, and can expect physiological functions such as estrogen. Antioxidant, antimicrobial and anti - cancer effects of isoflavones are well known. Recently, skin anti - aging efficacy of isoflavones has also been reported, which is a potential candidate for improving skin aging. These isoflavones have low solubility in water or oil and are difficult to apply to cosmetics at high concentrations.

Accordingly, the present invention has been made to solve the above-mentioned problems and to provide a cosmetic composition which is effective for preventing or ameliorating various skin aging symptoms due to reduction of female hormones, especially estrogen, after menopausal period.

The present invention also provides an estrogenic activity effect that is superior due to the synergistic effect and provides an optimal combination for use in cosmetic compositions.

In order to achieve the foregoing object, the present invention Sagan (Belamcanda Chinensis extract, genistein, and daidzein. The present invention provides cosmetic compositions for preventing or improving skin aging.

The inventors of the present invention have repeatedly studied a variety of plant extracts and synthetic compounds for improving skin elasticity, gloss reduction, wrinkling or dry feeling symptoms after menopausal period. As a result, it has been found that when the extracts of the present invention are used together with genistein and daidzein, they exhibit similar activities to estegens when used in an optimal ratio, and are effective for alleviating or improving the skin aging symptoms appear after the menopausal period. Moreover, Belamcanda Experiments have shown that the synergistic effects of estrogen-like effects are superior to those of chinensis extract, genistein and daidzein, respectively, when used together.

As used herein, the term "prevention " means preventing the appearance of skin aging symptoms that may occur due to menopausal symptoms as described above, and can be understood as a broad concept including prevention, treatment, and mitigation.

As used herein, the term "improvement" can be understood as a broad concept meaning treating the aging of the skin, which can be caused by menopause, and restoring the symptoms close to the original state.

It is known that the above-mentioned medicinal herb is dried rootstock of Belamcanda chinensis Leman., And has excellent antifungal, anti-viral, anti-inflammatory and hypotensive effects. Belamcandin, iridin, tectoridin, and tectorigenin are well known as anticholinergic and anti-inflammatory agents.

The extract of the present invention means an extract obtained by a conventional method in the field of the present invention and can be further purified by extraction, fractionation, separation, filtration and the like.

For example, the extract of the present invention may be a solvent extract thereof or a dried product thereof. The extraction solvent used in the extraction of dead cells may be any solvent which can be used for extracting natural products without limitation, for example, purified water; Lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; Hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether and dichloromethane; Or a mixed solvent thereof may be used, but it is preferable to use purified water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, butyl alcohol, or a mixed solvent thereof, and it is preferable to use purified water, ethanol, , And it is more preferable for the purpose of the present invention to use a mixed solvent of purified water and ethanol.

The amount of the extraction solvent may be 10 to 100 times, preferably 20 to 80 times, more preferably 30 to 50 times the weight of the extraction solvent, but is not limited thereto.

The method of extracting the above extract may be carried out by a conventional extraction method such as stirring, cold extraction, hot water extraction, immersion extraction, supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, And it is preferable to heat and reflux or extract at room temperature. The extraction temperature is preferably 10 to 30 ° C, and the extraction time is preferably 1 to 20 days, more preferably 1 to 5 days, but is not limited thereto.

More specifically, the suspension may be shredded and shredded, the extraction solvent may be added by 1 to 20 times the volume of the pulverized product to be extracted, and then selectively (decompression) concentrated, dried or purified. More specifically, an extraction solvent is added to extract an active ingredient substance as in the above-mentioned method, (a) heating at a temperature of 50 to 100 ° C for 1 to 20 hours in a state where a cooling condenser is installed to prevent evaporation of the solvent, (B) immersing the mixture at a temperature of 5 to 37 DEG C for 1 to 15 days to extract the active ingredient.

Further, according to another embodiment of the present invention, fractions of Leucine extract may be used.

The instant fraction of the present invention can be obtained by fractionating the instant extract (preferably a lower alcohol extract having 1 to 4 carbon atoms) with a solvent having a polarity lower than that of water such as hexane, chloroform, butanol and ethyl acetate, Or chloroform, but is not limited thereto.

Particularly, for the purpose of attaining the effect according to the present invention, it is preferable that the extract of the present invention is a hexane or chloroform fraction of a lower alcohol extract having 1-4 carbon atoms.

The fraction may be prepared by suspending or dissolving the dried extract in a liquid state before drying or in a suitable solvent such as water, ethanol, or a mixed solvent thereof, and then dissolving the dried extract in a suitable solvent such as hexane, chloroform, butanol or ethyl acetate This low solvent may be sequentially added to separate each layer, and then each layer may be obtained by concentration under reduced pressure and / or drying.

At this time, the vacuum concentration is preferably performed using a vacuum rotary evaporator, but not limited thereto.

In addition, " drying " according to the present invention may be vacuum drying, vacuum drying, boiling drying, spray drying, room temperature drying or freeze-drying, However, the present invention is not limited to this drying method.

Genistein is a compound represented by the following general formula (1), which is a kind of isoflavone and can be obtained by soybean extract or by synthesis.

[Chemical Formula 1]

Daidzein is a compound represented by the following general formula (2), which is a kind of isoflavone as well as the above-mentioned genistein, and can be obtained by soybean extract or synthesis.

(2)

The inventors of the present invention have confirmed that the above-mentioned genistein and daidzein exhibit similar activities to estrogen, which shows excellent effects in improving or preventing skin aging in menopausal period. Furthermore, as a result of conducting experiments on natural plants capable of imparting synergistic effects to the estrogenic activities of the above-mentioned genistein and daidzein, it was confirmed that the extracts of the present invention showed the best synergy effect, .

Specifically, the inventors of the present invention have confirmed through experiments that the synthesized genistein and daidzein exhibit estrogen-like activity when used in combination with the extract of Mishima and are useful for improving the skin condition of menopausal women.

The cosmetic composition of the present invention contains 0.0001 to 0.5% by weight, preferably 0.0005 to 0.1% by weight, more preferably 0.001 to 0.05% by weight, based on the total weight of the composition, of genistein and daidzein. If the content is less than 0.0001% by weight, the desired estrogenic activity can not be sufficiently attained, which is undesirable. If the content exceeds 0.05% by weight, an increase in the effect is not expected to increase markedly, It is possible to cause a problem in the stability of the apparatus.

In addition, the extract may contain 0.1 to 10% by weight, preferably 0.5 to 8% by weight, more preferably 1 to 5% by weight, based on the total weight of the cosmetic composition comprising the genistein and daidzein .

If the content is less than 0.1% by weight, it may be difficult to achieve synergistic effects with the genistein and daidzein, so that it may be difficult to achieve the object of the present invention. When the content is more than 10% by weight, Is not expected to be economical.

The cosmetic composition comprising the mixture of the extract of the present invention, genistein and daidzein according to the present invention may further contain an excipient or an additive, and is not particularly limited as long as it is an excipient or an additive which can be included in a conventional cosmetic composition.

The composition for preventing or improving menopausal skin according to the present invention further comprises ingredients commonly used in the cosmetic composition as an active ingredient and includes conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances, And a carrier.

The present invention relates to a cosmetic composition for preventing menopausal skin aging, which is characterized in that the cosmetic composition is any one selected from the group consisting of cosmetics, essences, skins, lotions, creams, packs, foundations, Which is characterized in that a cosmetic composition containing a mixture of an extract of Chrysanthemum indicum, genistein and daidzein is used in the production of a cosmetic base product or a color cosmetic product, .

When the formulation of the cosmetic composition of the present invention is a paste, a cream or a gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide Can be used.

When the formulation of the cosmetic composition of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, Propellants such as carbon, propane / butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a solution or an emulsion, a solvent, a solubilizer or a emulsifier is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Glycol, dipropylene glycol, butylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, PEG-8, polyethylene glycol or sorbitan.

In addition, the cosmetic composition of the present invention can be applied in various forms such as liquid, cream, paste, and solid forms, and can be manufactured using conventional cosmetic preparation methods.

The formulation of the cosmetic composition of the present invention is not particularly limited in shape as described above. However, the object of the present invention is to improve the skin elasticity, gloss reduction, wrinkle and dry feeling due to skin aging of the skin during menopause, Such as ointments for external use on the skin, lotions, and the like.

The cosmetic composition of the present invention may be applied to the skin alone or in combination with a carrier or the like, and may be administered at least once at the time of application or administration.

The composition comprising all of the extracts of the instant invention, genistein and daidzein exhibited estrogen-like activity due to the synergistic effect of the above extract, genistein and daidzein mixture, It was confirmed that it exerts more excellent effect.

The composition comprising all of the extract of the present invention, genistein and daidzein has the effect of alleviating and / or alleviating the symptoms of skin aging which may appear in a woman's menopausal period.

The mixture of the above extracts, genistein, and daidzein can exhibit estrogen-like activity synergistically.

The cosmetic composition containing the mixture may have various effects of preventing or ameliorating various symptoms of skin aging that may be caused by skin aging of the skin such as reduction of elasticity, decrease of gloss, wrinkle formation and severe dry feeling.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments. The embodiments of the present invention are provided by way of example to facilitate a specific understanding of the present invention.

Example One. Estrogenic Comparison of active effects

In order to find a composition capable of preventing or ameliorating skin aging in menopause, in order to find an isoflavone compound having an estrogen-like effect, a luciferase assay, which activates a transcriptional promoter by reacting with an estrogen receptor to produce a fluorescent protein, Activity was measured. NIH3T3 cells were transfected with vectors having an ER response element capable of reacting with estrogen receptors. After 24 hours, the estrogenic activity was measured by luciferase assay. The amount of luminescence of the control group was changed to 1 and the estrogenic activity for each condition was examined.

Comparison of estrogenic activity effects of single and multiple treatments of genistein, daidzein, and sajan extracts Condition Luciferase Activity (fold) Control group 1.0 Positive control (treatment with estrogen 0.1 / / ml, optimal concentration in cells) 2.1 Genistein 0.1 (占 퐂 / ml)
1.0 ([mu] g / ml)
10.0 ([mu] g / ml) 1.3
1.9
2.0 0.1 [mu] g / ml of daidzein
1.0 ([mu] g / ml)
10.0 ([mu] g / ml) 1.4
2.2
2.4 0.5%
1.0 wt%
2.0 wt% 1.2
1.5
1.6 Genistein (0.1 占 퐂 / ml) + daidzein (0.1 占 퐂 / ml) 2.5 (0.1 μg / ml) + daidzein (0.1 μg / ml) + goethite extract (1% 2.9

As shown in the above Table 1, synergistic effects were shown to be superior to those when using each of genistein and daidzein, respectively.

Specifically, the luciferase activity was 1.9 and 22 when the above-mentioned 0.1 μg / ml of genistein and 0.1 μg / ml of daidzein were used, respectively, 10 times, that is, 1.0 μg / ml.

However, when the total amount of each of the above-mentioned genistein and daidzein was 0.1 μg / ml and the total amount was 0.2 μg / ml, they had a luciferase activity of 2.5.

That is, it can be seen that the use of both genistein and daidzein can achieve a further improved effect even if the amount is reduced to 1/5.

Therefore, it was found that when lycopene is included together with genistein and daidzein, it has excellent luciferase activity.

In addition, it has been confirmed through experimentation that the extract of Zymogen increases the luciferase activity with the above-mentioned genistein and daidzein.

More specifically, the Luciferase Activity did not show a significant increase from 1.5 to 1.6 even when the amount of quercetin was doubled.

However, the use of genistein and daidzein together with the extract of Zymogen showed a significant improvement of the luciferase activity to 2.9.

As a result, it was expected that Luciferase activity could be expected to be improved when genistein and daidzein were used together with the extract of Zanthoxylum.

It is also believed that a composition comprising both the genistein extract and the genistein and daidzein therewith will have excellent estrogenic activity.

This was superior to the highest efficacy of the individual components and was superior to the activity when estrogen was most effective.

Formulation Example

Formulation Example 1. A gel containing the genistein, daidzein and triglyceride extracts was prepared according to a conventional method as shown in Table 2 below.

Raw material name Example 1 (% by weight) Comparative Example 1 (% by weight) Dipropylene glycol 10.0 10.0 Figure-8 8.0 8.0 Butylene glycol 6.0 6.0 PIGGY-40 Hydrogenated Castor Oil 0.4 0.4 Daid Jane 0.01 - Genestine 0.02 - Sangan Extract 2.0 - Carbomer 0.4 0.4 Triethanolamine 0.4 0.4 antiseptic Suitable amount Suitable amount Spices Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water to 100 to 100

Formulation Example 2. Lotions containing the genistein, daidzein, and menthae extracts as shown in Table 3 below were prepared according to a conventional method.

Raw material name Example 2 (% by weight) Comparative Example 2 (% by weight) Cetostearyl alcohol 1.0 1.0 Glyceryl stearate 1.0 1.0 FAGE -100 stearate 1.0 1.0 Sorbitan sesquistearate 1.5 1.5 Hexyldecyl hexanoate 5.0 5.0 Caprylic / capric glyceride 4.0 4.0 Triethanolhexanone 3.0 3.0 Dipropylene glycol 10.0 10.0 Figure-8 8.0 8.0 Glycereth-26 5.0 5.0 Daid Jane 0.01 - Genestine 0.02 - Sangan Extract 2.0 - Carbomer 0.1 0.1 Triethanolamine 0.1 0.1 antiseptic Suitable amount Suitable amount Spices Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water to 100 to 100

Formulation Example 3. An essence containing the genistein, daidzein and triglyceride extracts was prepared according to a conventional method as shown in Table 3 below.

Raw material name Example 3 (% by weight) Comparative Example 3 (% by weight) Denatured alcohol 5.0 5.0 Dipropylene glycol 10.0 10.0 Figure-8 10.0 10.0 Butylene glycol 5.0 5.0 PIGGY-40 Hydrogenated Castor Oil 0.4 0.4 Daid Jane 0.02 - Genestine 0.03 - Sangan Extract 2.0 - Carbomer 0.2 0.2 Xanthan gum 0.1 0.1 Acrylate / C10-30 alkyl acrylate crosspolymer 0.1 0.12 Triethanolamine 0.3 0.3 antiseptic Suitable amount Suitable amount Spices Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water to 100 to 100

Formulation Example 4. Cream containing the genistein, daidzein and triglyceride extracts was prepared according to a conventional method as shown in Table 3 below.

Raw material name Example 4 (% by weight) Comparative Example 4 (% by weight) Cetostearyl alcohol 2.0 2.0 Glyceryl stearate 1.5 1.5 FAGE -100 stearate 1.0 1.0 Stearic acid 0.5 0.5 Polyglyceryl-3 methyl glucoside distearate 1.5 1.5 Squalane 10.0 10.0 Macadamia seed oil 5.0 5.0 Isocetyl octanoate 5.0 5.0 Dipropylene glycol 10.0 10.0 Figure-8 8.0 8.0 Butylene glycol 4.0 4.0 Daid Jane 0.01 - Genestine 0.02 - Sangan Extract 2.0 - Carbomer 0.1 0.1 Triethanolamine 0.1 0.1 antiseptic Suitable amount Suitable amount Spices Suitable amount Suitable amount Pigment Suitable amount Suitable amount Purified water to 100 to 100

Clinical effect data

In the cosmetic composition of the present invention, in which the genistein, daidzein and extracts of the present invention were added, the efficacy of the cream for improving wrinkles, elasticity, moisturizing, glossiness and sensitivity in the skin was evaluated.

Clinical trial example 1. Wrinkle improvement effect

Evaluation items Improvement rate (%) wrinkle 6.9% (after 8 weeks, visiometer measurement)

As shown in Table 6, when the cosmetic composition of the present invention was used for 8 weeks, the average skin wrinkle improvement rate of participants was 6.9%. Therefore, it was found that the composition of the present invention has an excellent effect on the improvement of skin wrinkles due to aging of the skin during the menopausal period.

Clinical trial example 2. Elasticity improvement effect

Evaluation items Improvement rate (%) elasticity 5.7% (after 4 weeks use, Balistometer measurement)

As shown in Table 7, when the cosmetic composition of the present invention was used for 4 weeks, the average skin elasticity improvement rate of participants was 5.7%. Therefore, it was found that the composition of the present invention has an excellent effect in improving skin elasticity due to skin aging of menopausal age.

Clinical trial example 3. Moist improvement effect

Evaluation items Improvement rate (%) Moisturizing 14.1% (Corneometer measurement after 4 weeks use)

As shown in Table 8, when the cosmetic composition of the present invention was used for 4 weeks, the average skin moisturizing degree of the participants was improved by 14.1%. Therefore, it was found that the composition of the present invention improves moisture deficiency due to aging of the skin of menopausal women and has an excellent effect on moisturizing.

Clinical trial example 4. Glossiness improvement effect

Evaluation items Improvement rate (%) burnish 9.5% (after 4 weeks, measured by Glossimeter)

As shown in Table 9, when the cosmetic composition of the present invention was used for 4 weeks, the average skin gloss increase rate of participants was 9.5%. Therefore, it was found that the composition of the present invention is effective in improving the skin roughness caused by aging of the skin during the menopausal period and providing the skin with shine.

Clinical trial example 5. Sensitivity improvement effect

Evaluation items Improvement rate (%) responsiveness 85.4% (after 4 weeks, measured by Neurometer CPT)

As shown in Table 10, when the cosmetic composition of the present invention was used for 4 weeks, the average skin sensitivity of the participants was improved by 85.4%. Therefore, it was found that the composition of the present invention is effective in improving symptoms of skin becoming sensitive due to aging of the skin during menopause.

As a result of using the cream added with the extracts of genistein, daidzein and japonica extract in the menopausal women, as shown in Tables 6 to 10, the improvement was significantly improved as compared with the case of not used in all the items below.

Claims

Belamcanda Chinensis extract, genistein, and daidzein.

2. The cosmetic composition according to claim 1, wherein the genistein and daidzein are not derived from an extract from a dead skin.

The cosmetic composition according to claim 1, wherein the menopausal skin aging includes symptoms of skin elasticity reduction, gloss reduction, wrinkle formation or dry feeling.

[3] The cosmetic composition according to claim 1, wherein the cosmetic composition comprises 0.1 to 5%
And 0.000001 to 0.01% by weight of said genistein and daidzein, respectively.

A cosmetic composition for preventing or improving skin aging, which comprises genistein and daidzein.