KR101843065B1 - A composition for anti-aging comprising mixture of carnosine, soypeptide and andrographis paniculata extract - Google Patents

Abstract

The anti-aging cosmetic composition comprising the canosin, soybean peptides and extracts of Tessykimin as an active ingredient of the present invention exhibits an effect of promoting DEJ and ECM as well as an effect of inhibiting cell senescence by oxidative stress, And it is expected that it can be effectively used as a cosmetic composition and food composition for anti-aging because there is no toxicity to cells as well as effectively improving skin aging.

Description

[0001] The present invention relates to an anti-aging composition comprising a carnosine, a soybean peptide, and a tsunami extract,

The present invention relates to an antioxidant composition comprising carnosine, soybean peptides and extracts of Tessin, and more particularly to a composition for improving dermal epidermal junction (DEJ) protein, an ECM (Extracellular matrix) An anti-aging cosmetic composition or a food composition comprising a carnosine, a soybean peptide, and a cinnabar extract which exhibits an effect of inhibiting cellular senescence and an effect of increasing the reactivity of a skin active growth factor.

Skin is the primary organ of aging, and much research has been done in the field of cosmetics to delay or prevent it. Skin aging usually begins around the age of 25, and skin aging proceeds in earnest at around the age of 40 years. Skin aging is divided into photoaging and endogenous aging. In the case of endogenous aging, it is a natural phenomenon due to degenerative changes that occur in proportion to the progress of time with age. Skin external changes related to skin aging include skin dryness, skin sagging and wrinkles, and skin diseases include bruising keratosis and black spots. Skin aging is a phenomenon occurring in each layer of the skin, dermis collagen, GAG, including ECM (extracellular matrix) production is reduced due to the dermis is atrophied, the skin is also thin and dry skin. Dermal epidermal junction (DEJ) proteins responsible for epidermal and epidermal junctions also decrease in expression as the aging progresses, resulting in flattening of the epidermal-dermal junction and weakening of the connection, resulting in easy skin damage and permanent supply And the aging of the skin is promoted. It is also reduced by external factors such as ultraviolet light. DEJ-related proteins include collagen 4, collagen 7, collagen 17, and Lminin 5. Active oxygen, a typical inducer of extrinsic aging, occurs during ultraviolet light, pollution, inflammation, and cell-mediated metabolism, and promotes cell senescence.

When cell senescence is promoted, the ability to regenerate cells and the ability of skin constituents such as collagen, elastin, and hyaluronic acid to degrade. In addition, the decrease in growth factor receptor due to aging may cause a decrease in reactivity to external growth factors, and the growth factors that activate cells often fail to function properly. In fact, studies have been reported that fibroblasts with progressive cell senescence do not respond to treatment with EGF, while receptors for EGF (epidermal growth factor), a typical skin activator, decrease [Hidenori Shiraha et al, JBC, 275 ; 19343-19351, 2000). Therefore, it can be inferred that suppression of cell senescence is a major anti-aging strategy in order to respond appropriately to cell activators such as EGF.

Therefore, the inventors of the present invention have continued to develop a composition for anti-aging which can simultaneously inhibit the aging of epidermal-dermal junctions (DEJ) and dermis in a naturally occurring compound having high safety and safety, , Canosin, soybean peptide, and Tessy's extract can simultaneously exhibit the effect of inhibiting cell senescence by oxidative stress, increasing the activity of skin activating growth factor, as well as enhancing the effect of DEJ and enhancing ECM. Completed.

Accordingly, a technical problem to be solved by the present invention is to provide a composition having anti-aging activity.

In order to solve the above technical problems, the present invention provides an anti-aging cosmetic composition comprising as an active ingredient an extract of canosin, a soybean peptide, and a tsunami extract.

In addition, the present invention provides an anti-aging food composition containing canosin, soybean peptides, and tsunami extract as an active ingredient.

Carnosine, an active ingredient of the anti-aging composition of the present invention, is a dipeptide of β-alanyl-L-histidine composed of β-alanin and histidine, Significant amounts are found, especially in the non-protein portion of vertebrate muscle cells, which amount to about 1 to 20 mM. Kanosin is an antioxidant that protects cells from oxidative stress and is known as a free radical scavenger that has an excellent effect in removing reactive oxygen species (Boldyrev et al., Neuroscience 94 2): 571-577, 1999, USP 5,866,537, USP 5,503,776). Taking advantage of its function as an antioxidant, kanosin has been mainly used as a cosmetic or dermatological therapeutic agent (USP 5,723,482; USP 5,710,177). The protective action of canosin is achieved by inhibiting the deformation of biopolymers in oxidative stress environment through proton buffering, heavy metal chelating and elimination of free radicals and active sugar molecules and maintaining their inherent functions (Boldyrev et al., Biochemistry (Mosc) 65 (7): 751-756,2000).

In the present invention, Andrographis paniculata is a perennial plant of Acanthaceae that grows predominantly throughout India, especially in the southern fields and forests where shade and moisture are sufficient. Its height is 0.3-1.0 meters. The stem is dark green with a thickness of 2-6mm. Leaves are 8cm long and 2.5cm wide, with a shallow-leaf shape. Flowers are white or have purple spots on a pinkish petal. It is known as 'Maha-tita' (King of bitters) because it tastes extremely tasteless in all plant parts. It is also called 'bhui-neem' because of its similar shape and bitter taste. These tsunami medicines have been known for their anti-cancer, anti-inflammatory, antibacterial and antiviral actions and have been used for immunization, budding,

The extract of the present invention is a substance which extracts, extracts, leaves, roots, stems, flowers and the like of the above-mentioned Tensilaceae by an ordinary method or has an anti-aging activity separated therefrom and improves the skin condition. In addition, the above-mentioned extract refers not only to the extract obtained from the extract of Tenshi extract by conventional methods, but also to its dry powder or any form which is formulated using the same.

In the present invention, the extract can be extracted using water or an organic solvent. The extracted liquid can be used in a liquid form or can be used by concentration and / or drying. The organic solvent may be selected from the group consisting of methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethylsulfoxide , 3-butylene glycol, propylene glycol, or a mixed solvent thereof. The extract can be extracted at room temperature or with heating under the condition that the active ingredient of the extract is not destroyed or minimized. Depending on the organic solvent to be extracted, the degree of extraction and the degree of loss of the active ingredient of the extract may differ. Therefore, an appropriate organic solvent should be selected and used. The extraction method is not particularly limited, and examples thereof include cold extraction, ultrasonic extraction, and reflux cooling extraction.

In the present invention, the tsunami extract includes all of the extract, fraction and purified product obtained in each step of extraction, fractionation or purification, and a diluted solution, a concentrate or a dried product thereof.

The soybean peptide, which is an active ingredient of the anti-aging composition of the present invention, is a degradation product of a protein hydrolyzed partly from a soybean protein and has physiological activity and is composed of peptides that are water-soluble, It has been reported that it has the effect of increasing DEJ. The soybean peptide used in the present invention can be used in the form of a solution or a powder, and can be commercially available or can be directly isolated from soybeans by a known method. Examples of the method for producing the soybean peptide solution include mixing the soybean peptide and the water at a weight ratio of 5 to 10: 90 to 100, preferably 5: 100, and in the present invention, the commercially available liquid soybean peptide is dissolved in 70 And then adding the solution to the purified water which has been heated up to 80 ° C, stirring and continuing to agitate for 10 to 20 minutes and cooling.

In the present invention, when carnosine, corticosteroid extract and soybean peptide are mixed with each other, the cell senescence inhibitory effect by oxidative stress and the reactivity of the skin activating growth factor are increased in comparison with the case of using the canosin, cinnabar extract or soybean peptide Not only the effect but also the DEJ enhancing effect and the ECM enhancing effect can be remarkably increased.

Specifically, the cosmetic composition for anti-aging according to the present invention may be used in a weight ratio of 0.9 to 1.1: 0.4 to 0.6: 0.9 to 1.1, preferably 1: 0.5: 1, . Hereinafter, an anti-aging complex compound comprising an active ingredient of the composition of the present invention, namely, canosin, cinnabar extract, and soybean peptide is referred to as "AC" (Anti-aging complex).

According to one specific embodiment, the concentrations of cannosine, TCE extract and soybean peptide were found to be inoculated with no intracellular toxicity, and were found to be 50 ppm, 20 ppm and 50 ppm, respectively, and mixed at a weight ratio Compounds, and their ROS (reactive oxygen species) scavenging ability, collagen synthesis test, and DEJ-related protein expression were measured. As a result, the complex compound containing canosin, Tenshi extract and soybean peptide inhibited the ROS activity increased by external oxidative stress (rothenone) by at least 20%, and the synthesis of collagen, which is one of the main components of ECM, At least 30% more, and that the expression of DEJ-related protein was increased by at least 50%.

The composition of the present invention contains 0.0001 to 20% by weight, preferably 0.001 to 10% by weight, more preferably 0.01 to 10% by weight, based on the total weight of the composition, of a complex compound (AC) of canosin, cinnabarin extract and soybean peptide 5% by weight. If the weight of the AC is less than 0.0001 wt%, the effect of preventing aging of the skin is insufficient. If the weight of the composite compound is less than 20 wt%, the effect of increasing the content is very weak.

In addition, according to one preferred embodiment of the present invention, an EGF (Epidermal Growth Factor), an FGF (Fibroblast Growth Factor) and the like may be further added to the anti-aging composition of the present invention in order to increase the anti-aging activity.

The composition containing AC of the present invention exhibits an anti-inflammatory effect and has little cytotoxicity as a natural substance. According to one embodiment of the present invention, there is provided a cosmetic composition comprising AC as an active ingredient.

The cosmetic composition according to one embodiment of the present invention may contain conventional additives such as anti-inflammatory agents, antioxidants, stabilizers, solubilizers, vitamins, pigments and fragrances in addition to AC as an active ingredient, The carrier may be further added.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it can be prepared as a nutritional cream, a convergent lotion, a soft lotion, a lotion, an essence, a nutritional gel or a massage cream.

When the formulation of the present invention is a paste, cream or gel, use is made of an animal oil, vegetable oil, wax, paraffin, starch, tragacanth gum, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Castellulose, aluminum metahydroxide, bentonite, agar or tracert, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

In addition, the food composition according to one embodiment of the present invention may further contain, as an active ingredient, not only AC but also components that are ordinarily added in food production, such as protein, carbohydrate, fat, nutrients, seasoning, .

Examples of such carbohydrates are monosaccharides, such as glucose, fructose, and the like; Disaccharides such as maltose, sucrose, oligosaccharides and the like; And polysaccharides such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavorings such as tau martin and stevia extract (e.g., rebaudioside A and glycyrrhizin) and synthetic flavorings (saccharine, aspartame, etc.) can be used as flavorings.

For example, when the food composition of the present invention is prepared as a drink, it may further contain citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, mulberry extract, jujube extract, licorice extract etc. in addition to AC of the present invention .

Meanwhile, as a result of the skin irritation test of the complex compound in the specific examples of the present invention, it has been found that the complex compound is a harmless substance to the human body as a natural substance. Accordingly, the complex compound prepared by mixing the canosin, cinnabar extract and the soybean peptide of the present invention is harmless to the human body and has little toxicity and side effects, so that it can be safely used even in long-term use. In particular, Can be safely applied to the composition.

As described above, the anti-aging composition comprising the canosin, soybean peptide, and tsunami extract of the present invention as an active ingredient has an effect of inhibiting cell senescence due to oxidative stress, increasing the activity of skin activating growth factor, Can effectively improve skin aging, and is not toxic to cells. Therefore, it is expected to be useful as a cosmetic composition and food composition for anti-aging.

FIG. 1 is a graph showing the effect of increasing the collagen production of a single treatment group of canosin, cynomolium extract and soybean peptide, and a complex compound (AC) thereof.
FIG. 2 is a graph showing the effect of increasing the expression of DEJ protein in the monocotyledonous extract, soybean peptide extract, and complex compound (AC) thereof.
FIG. 3 shows the effect of increasing the collagen production in the fibroblast cells induced by oxidative stress and the complex compound (AC) of the monocotyledonous extract, the soybean peptide extract and the soybean peptide monoclonal antibody.
FIG. 4 is a graph showing the wrinkle-reducing effect in a short-term clinical test of a compound (AC) of a canosin, a tsunami extract and a soybean peptide.

Hereinafter, embodiments of the present invention will be described in detail to facilitate understanding of the present invention. However, the embodiments according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the invention are provided to more fully describe the present invention to those skilled in the art.

<Preparation Example 1> Preparation of Tengshin extract

The supernatant fluidized bed reactor (Natex, Austria) was charged with 1 kg of the dried sample and 500 to 700 ml of ethanol (99.5% Under the condition that the pressure was 10 MPa and the S / F ratio (Supercritical fluid kg / Feed kg) was 35, the total carbon dioxide was extracted using 40 kg of carbon dioxide. The mixture of carbon dioxide, ethanol and feedstock was then passed through a separator and the extracts were separated from the carbon dioxide by varying the pressure and temperature of the mixture. The extract was concentrated under reduced pressure to remove ethanol, and 121.4 g of tsunami extract was prepared.

<Preparation Example 2> Preparation of a composition containing canosin, cinnabar extract and soybean peptide

A mixture containing canosin, Tenshi extract, and soybean peptide was mixed at a weight ratio of 1: 0.5: 1 to prepare a composition containing a complex compound (AC) of canosin, Tenshi extract and soybean peptide.

&Lt; Example 1 > Composition cytotoxicity test

The cytotoxicity test (MTT) of the composition was performed using human-derived fibroblasts. The cytotoxic effect of the cells was measured by MTT (3- (4-hydroxyphenyl) -1- (4-methylphenyl) , 5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide) analysis method. Cell viability was expressed as percentage of negative control, and the results are shown in Table 1.

At the concentration of 50 ㎍ / ㎖, there was a significant decrease in the number of cells compared to the negative control and no significant cell number change was observed in the kanosin and soy peptide treated groups. No significant changes in cell survival were observed after treatment with maximal concentrations that did not show cytotoxicity for each treatment substance. The concentration of the complex compound was 50 μg / ml, 50 μg / ml, and 25 μg / ml, respectively.

&Lt; Example 2 > Measuring ROS scavenging ability

Human normal fibroblasts were inoculated into a 24-well microplate containing DMEM medium to a concentration of about 3 x 10 ⁴ cells and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours Lt; / RTI &gt; The test material was then treated and further incubated for 24 hours. The cells were further treated with 100 mM H2DCFDA (H2DCFDA (Molecular Probe, USA)) capable of measuring intracellular ROS levels and rotenone (200 mM) capable of inducing intracellular oxidative stress and then re-cultured for 30 minutes. Subsequently, the cells were pulverized with 1N NaOH and the degree of fluorescence present in the cell lysate was measured using a fluorometer instrument (Infinite M200, Tecan, Switzerland). The ROS scavenging results are shown in Table 2.

Treating material (-) Canosin
50 μg / ml The
extract
25 / / ml Big head
Peptides
50 μg / ml (Canosin + TCE + soybean peptide) complex (AC) ROS scavengers 0 20% 8% 28% 41%

As shown in Table 2 above, the ROS scavenging activity of canosin, Tenshi extract and soybean peptide was 20%, 8%, and 28%, respectively. In the case of using a mixture of these substances, the ROS scavenging ability was increased to 41% Respectively. This means that the efficacy can be elevated when the major constituents of the present invention are combined at a concentration without cytotoxicity.

&Lt; Example 3 > In vitro collagen synthesis performance measurement

The fibroblast present in the dermis is a cell that produces ECM, a major constituent of the dermis. The protein known to be involved in the skin wrinkles of the ECM constituent is type-1 procollagen. The wrinkle-reducing effect of the test substance was observed through the type-1 collagen collagen biosynthesis performance. Human normal fibroblasts were inoculated into a 24-well microplate containing DMEM medium (2 x 10 ⁵ cells / well) and cultured in a 5% CO ₂ incubator at 37 ° C for 24 hours Lt; / RTI &gt; After 24 hours, the medium was removed from each well, samples were treated at different concentrations, and then cultured again for 24 hours. After 24 hours, the cell culture medium was collected to prepare a sample. The amount of collagen synthesis was determined by measuring the amount of procollagen type I C-peptide (Type I C-peptide: PICP) using a collagen measurement kit (Procollagen Type I C-peptide EIA kit MK101, Takara, Japan). Specific test methods were performed according to the instructions of Takara Co., Ltd.'s kit. Collagen assembly performance results are shown in FIG.

Experimental results showed no significant effect of canosin and cyanobacterium extracts on the treated concentration range, and soy peptides were found to increase the type - 1 procollagen collagen synthesis performance to 16%. When using these substances in combination, it was confirmed that the collagen synthesis ability increased by 35%. This means that the efficacy can be elevated when the major constituents of the present invention are combined at a concentration without cytotoxicity.

<Example 4> Measurement of DEJ-related protein aggregation performance

Studies on inhibitors of skin aging at the dermal epidermic junction (DEJ) have been spotlighted. Collagen-4, 7, 17, laminin-5 And the like. In the case of the DEJ protein biosynthesis performance experiment, human normal fibroblasts were inoculated (2 x 10 ⁵ cells / well) into a 24-well microplate containing DMEM medium, and 5% CO ₂ Lt; RTI ID = 0.0 &gt; 37 C &lt; / RTI &gt; for 24 hours. After 24 hours, the medium was removed from each well, samples were treated at different concentrations, and then cultured again for 24 hours. After 24 hours, the cell culture medium was collected to prepare a sample. Collagen 4, 7, 17, and laminin production were measured using an ELISA kit (Cloud-Clone Corp., USA). Detailed test methods were performed according to the instructions of the analysis kit of Cloud-Clone Corp. The result of DEJ protein synthesis is shown in Fig.

As shown in FIG. 2, when the AC-containing composition of the present invention was treated with collagen-7, 17 and laminin-5 except for collagen-4, the amount of protein expression was increased synergistically. In particular, an increase in the expression level of collagen-7 and collagen-17 was observed to be remarkable.

&Lt; Example 5 > Antioxidant activity of complex compounds Measure

The antioxidant activity of the antioxidant composition of the present invention has an effect of inhibiting cell senescence induced by oxidative stress. In addition, the anti-aging composition of the present invention can inhibit cell senescence I looked at it.

The fibroblasts were treated with 0.5 μM of rhenone, an oxidative stress inducer, for 2 weeks to induce premature cell senescence. The test substance was treated with rothenone and after 2 weeks, the degree of cell senescence was observed through SA-βgal analysis. SA-βgal analysis was analyzed by FACS through C12FDG staining. The results are shown in FIG. As a result, it was observed that the complex mixture for anti - aging significantly inhibited oxidative stress - induced cell senescence.

Example 6 Measurement of Growth Factor Reactivity Enhancement Effect of Anti-Aging Complex Compound

As the cell aging progresses, the receptors for the cell growth factor decrease and the reactivity to the cell growth factor decreases. That is, even if EGF or FGF, which is widely used as an anti-aging agent in the skin field, is applied to the skin, aged cells do not react properly and it is difficult to obtain expected effects. In this experiment, we investigated the effect of anti - aging complex compounds inhibiting cell senescence on fibroblast cells induced early senescence by oxidative stress, which can increase skin growth factor responsiveness. The cells obtained by repeatedly treating the rottenone for 2 weeks and the cell group treated with the conjugate for rotonone and the anti-oxidative complex were inoculated in a 24-well microplate at 1 x 10 ⁵ cells / well, treated with EGF, a typical skin cell growth factor, Respectively. The results are shown in Table 3 below.

Untreated group 2-week treatment with rotenone
(Early induction of cell senescence) 2-week treatment with rothenone + anti-aging complex compound Cell proliferation increase rate at 5 ng / mL of EGF treatment 29% 8% 21%

As shown in Table 3, the increase rate of cell proliferation to EGF in the group treated with the anti-aging complex compound according to the present invention is significantly higher than the increase rate of cell proliferation according to EGF treatment of the fibroblasts induced by oxidative stress Were observed.

&Lt; Example 7 > Measurement of wrinkle-improving effect of cosmetics containing anti-aging compound

The effect of improving the wrinkles of cosmetic compositions containing the anti-aging complex compound was measured through clinical studies. The nutritional cream prepared in Production Example 1 (nutritional cream containing 0.05% each of anti-aging complex compound) was used.

The wrinkle improvement effect was analyzed by the Fringe projector (DermaTOP system, Ecotech company, France). The skin roughness factors were expressed by Ra, Rz, Rt parameters. The skin wrinkle improvement measurement was performed on 23 healthy women (35 to 60 years old) under the condition that the temperature was kept constant at 24 to 26 DEG C and the humidity was 38 to 40%. Production Example 1 The nutritional cream was applied to the face of the subject for 1 day Twice for 28 days after use, the degree of improvement of the wrinkles around the eyes was measured. The test results are shown in Fig.

As shown in FIG. 4, when the cream containing the anti-aging compound was applied for 1 month, the value of the parameter representing skin roughness was significantly decreased. Thus, it can be concluded that the anti-aging complex compound is effective for improving the skin wrinkles.

Formulation Example 1 Preparation of a cream containing an anti-aging complex compound

The composition of the nutritional cream containing the anti-aging complex compound is shown in Table 4 below. The aqueous phase, purified water, triethanolamine and propylene glycol were heated and dissolved at 70 DEG C, and a fatty acid, an oily component, an emulsifier and an antiseptic agent were heated to 70 DEG C to dissolve the emulsified liquid. After the emulsification was completed, the solution was cooled to 45 DEG C, and the complex compound and the fragrance for anti-aging were added and dispersed, followed by cooling to 30 DEG C. [

ingredient Content (% by weight) Compound for anti-aging 0.05 Jojoba oil 5.0 Liquid paraffin 7.0 Cetylaryl alcohol 2.0 Polyglyceryl-3 methylglucose distearate 2.0 Glyceryl stearate 0.5 Squalane 3.0 Propylene glycol 4.0 glycerin 5.0 Triethanolamine 0.3 Carboxyvinyl polymer 0.3 Tocopheryl acetate 0.2 Preservative, incense a very small amount Purified water Balance Sum 100

&Lt; Test Example 1 > Experiments to confirm the safety of the complex composition for anti-aging against human skin

In order to confirm whether the composite composition for antiaging agent is safe for human skin, a skin safety verification test was conducted. As a test method, a normal cumulative skin irritation test was performed.

1-1. Preparation of external skin preparation containing compound (AC)

In order to confirm that the complex compound (AC) is safe for the human skin, skin externalities containing the compound (AC) were prepared according to the ingredients and contents shown in Table 5, and skin stability and clinical efficacy tests were conducted. First, purified water, glycerin, and butylene glycol were mixed and dissolved at 70 캜 (water part), and the other components except for the above three components and trimethanolamine were dissolved at 70 캜 (oil part). The oil part was added to a water part and stirred with a homomixer (Tokushu Kika, Japan) for primary emulsification, and trimethanolamine was finally added thereto. Next, the bubbles generated in the mixed solution were removed, and the mixture was cooled to room temperature to prepare an external preparation for skin.

1-2. Experiment of cumulative stimulation of skin

The skin external preparation prepared by the method 1-1 described above was applied to 30 healthy adults for 9 times and 24 hours cumulative pelletization on the upper forearm every other day to determine whether or not the compound (AC) Respectively. As a control, an external preparation for skin without squalene-based complex compound (AC) was used.

A pin chamber (Finn chamber, Epitest Ltd, Finland) was used as a method of applying the coating, and 15 μl of each of the external preparations was dropped into the chamber. The degree of reaction on the skin each time was scored using the following Equation 1, and the results are shown in Table 6 below.

At this time, a score of ± is given as 1, a score of 2 is given as +, and a score of 4 is given as ++, and a safe composition is determined when the average degree of reaction is less than 3.

As shown in Table 6, in the case of the control group, the test group 1 and the test group 2, the number of persons corresponding to ±, +, and ++ was 0, 0, and 0, respectively. As a result of calculation according to the above formula, the average degree of reactivity of the control group and the test groups 1 and 2 was 0.00 or less, all 3 or less.

&Lt; Formulation Example 2 > Preparation of cosmetic preparation

2-1. Production of flexible lotion

As shown in Table 7 below, a lotion lotion containing a complex compound (AC) as an active ingredient was prepared by a conventional method.

ingredient Content (% by weight) Compound (AC) 0.1 glycerin 3.0 Butylene glycol 2.0 Propylene glycol 2.0 Carboxyvinyl polymer 0.1 ethanol 10.0 Triethanolamine 0.1 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

2-2. Manufacture of nutrition lotion

A nutritional lotion containing the complex compound (AC) as an active ingredient was prepared according to a conventional method as shown in Table 8 below.

ingredient Content (% by weight) Compound (AC) 0.1 Wax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 5.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxyvinyl polymer 0.1 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

2-3. Manufacture of nutrition cream

A nutritional cream containing the complex compound (AC) as an active ingredient was prepared according to a conventional method as shown in Table 9 below.

ingredient Content (% by weight) Compound (AC) 0.1 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.5 Liquid paraffin 10.0 Squalane 5.0 Caprylic / capric triglyceride 5.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

2-4. Manufacture of massage cream

A massage cream containing the complex compound (AC) as an active ingredient was prepared according to a conventional method as shown in Table 10 below.

ingredient Content (% by weight) Compound (AC) 0.1 Wax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 Liquid paraffin 40.0 Squalane 5.0 Caprylic / capric triglyceride 4.0 glycerin 5.0 Butylene glycol 3.0 Propylene glycol 3.0 Triethanolamine 0.2 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

2-5. Manufacture of packs

A pack containing the complex compound (AC) as an active ingredient was prepared according to a conventional method as shown in Table 11 below.

ingredient Content (% by weight) Compound (AC) 0.1 Polyvinyl alcohol 13.0 Sodium carboxymethylcellulose 0.2 Allantoin 0.1 ethanol 5.0 Nonyl phenyl ether 0.3 Preservatives, micronutrients, trace fragrances and trace water Balance sum 100.0

As described above, the anti-aging composition comprising the canosin, soybean peptide, and tsunami extract of the present invention as an active ingredient has an effect of inhibiting cell senescence due to oxidative stress, increasing the activity of skin activating growth factor, Can effectively improve skin aging, and is not toxic to cells. Therefore, it is expected to be useful as a cosmetic composition and food composition for anti-aging.

Claims (4)

A cosmetic composition for anti-aging comprising as an active ingredient, a carnosine, a cinnabar extract and a soybean peptide, wherein the carnosine, cinnabar extract and soybean peptide are mixed in a weight ratio of 0.9 to 1.1: 0.4 to 0.6: 0.9 to 1.1 Wherein the anti-aging cosmetic composition is an anti-aging cosmetic composition. delete delete The method according to claim 1,
Characterized in that EGF (Epidermal Growth Factor) or FGF (Fibroblast Growth Factor) is further added.

Images