KR20150050235A - Skin conditioning composition comprising ethyl hexyl gallate - Google Patents

Abstract

The present invention relates to an external use skin composition and a cosmetic composition for anti-oxidizing, anti-aging, whitening and moisturizing of skin including ethylhexyl gallate as an effective ingredient, wherein ethylhexyl gallate increases and activats moisture holding capability via free radical scavengers, inhibition of collagenase expression, inhibition of melanin synthesis, inhibition of NO discharge of microphage cell lines and induction of keratinocyte differentiation. The ethylhexyl gallate according to the present invention is checked to have excellent effect in increasing the moisture holding capability via inhibition of collagenase expression, inhibition of melanin synthesis, inhibition of NO discharge of microphage cell lines and induction of keratinocyte differentiation, so it may be broadly and usably applied to a cosmetic composition or an external use skin composition for anti-oxidizing, anti-aging, whitening anti-inflammation or moisturizing of skin.

Description

[0001] The present invention relates to a skin conditioning composition containing ethyl hexyl gallate,

The present invention relates to a composition for external application for skin and cosmetic composition for skin antioxidation, anti-aging, whitening, anti-inflammation and moisturizing comprising ethylhexogalate as an active ingredient, Anti-aging, whitening, anti-inflammation, or moisturizing, comprising ethylhexylgalate having an activity of increasing water retention ability through inhibition of NO release of macrophage cell line and induction of keratinocyte differentiation, as an active ingredient, .

The human skin undergoes changes due to various internal and external factors as it gets older. In other words, internally, secretion of various hormones that regulate metabolism is reduced, the function of immune cells and the activity of cells are lowered, and the biosynthesis of immune proteins and biocompatible proteins necessary for the living body is reduced, and externally, ozone layer destruction As a result, the content of ultraviolet rays reaching the surface of the sunlight increases and the environmental pollution is further intensified, so that free radicals and active noxious oxygen are increased, thereby reducing the thickness of the skin and increasing the wrinkles. In addition, not only the elasticity is decreased but also skin color is dull, skin troubles frequently occur, and spots, freckles and black spots are also increased.

As the aging progresses, the contents and arrangement of collagen, elastin, hyaluronic acid, and glycoprotein, which constitute the skin, appear to change or decrease, and oxidative stress is caused by free radicals and active noxious oxygen (Barja et al., " Free radicals and aging & quot ;, Trends Neurosci., 27 (10): 595-600). In addition, most of the cells constituting the skin, by aging or ultraviolet rays, produce proinflammatory cytokines, which are known to cause inflammation The biosynthesis of Cox-2, cyclooxygenase is increased and the biosynthesis of matrix metalloproteinase (MMP), which is an enzyme that decomposes the skin tissue by these inflammatory factors, is increased, It is known that the production of NO (nitric oxide) by iNOS (inducible nitric oxide synthase) is increased, and the biosynthesis of the substrate material is decreased by the decrease of cell activity and microinflammation due to the natural progression of endogenous aging, Degradation and degeneration are accelerated by external factors such as increase of stress caused by harmful environment and increase of active oxygen species by sunlight, As quality is destroyed and thin is displayed to various symptoms of skin aging. Accordingly, it is a reality that prevents the phenomenon of aging, and the number of studies performed with respect to active ingredients which can improve.

On the other hand, active oxygen generated by various physical, chemical, and environmental factors such as in vivo enzymatic system, reductive metabolism, chemical agents, pollutants and photochemical reaction, etc. is non-selective, irreversible It is known to cause various diseases including cancer or aging cells by destructive action. In addition, lipid peroxides and various peroxides such as lipid peroxides produced as a result of lipid peroxidation by these active oxygen also cause oxidative destruction to cells and cause various dysfunctions and cause various diseases. Accordingly, antioxidants such as free radical scavengers or peroxidation-inhibiting substances are expected to be used as agents for inhibiting or treating various diseases caused by these oxides.

Under the above background, the inventors of the present invention have found that, by having a free radical scavenging, suppression of collagenase expression, suppression of melanin synthesis, suppression of NO release of macrophage cell line and induction of keratinocyte differentiation, In studying substances that can be used for whitening, anti-inflammation and moisturizing, ethyl hexyl gallate effectively inhibited collagenase synthesis, melanin synthesis inhibition, inhibition of NO release of macrophage cell line, and induction of keratinocyte differentiation The present invention has been completed.

It is an object of the present invention to provide an antioxidant, an anti-aging, whitening, anti-inflammatory and moisturizing effect of skin by suppressing collagenase expression, inhibiting melanin synthesis, inhibiting NO release of macrophage cell line and inducing differentiation of keratinocyte And to provide a composition for external application for skin and a cosmetic composition.

In order to solve the above problems, the present invention provides a composition containing ethyl hexyl gallate as an active ingredient. The composition of the present invention may be a composition for skin antioxidation, anti-aging, whitening, anti-inflammation and moisturizing.

The composition of the present invention not only exhibits antioxidative and anti-aging effects, but also exhibits whitening, anti-inflammation and moisturizing effects by containing ethylhexyl galeate, and can be used in various fields such as cosmetics, household products or medicine.

The ethylhexylgalate of the present invention is a substance having an IUPAC name of 2-ethylhexyl 3,4,5-trihydroxybenzoate, a chemical formula of C ₁₅ H ₂₂ O ₅ and a molecular weight of 282.33, which is produced by a chemical synthesis method or an enzyme But is not limited thereto.

In one embodiment of the present invention, ethylhexogalate of the present invention is synthesized by using ethyl hexanol and gallate using Tannase enzyme, and then purified and used.

The ethylhexogalate of the present invention is used in an amount of 0.0001 to 30% (w / v), preferably 0.01 to 20% (w / v), more preferably 0.1 to 10% (w / v) . If the content of the active ingredient is less than 0.0001%, the effect of antioxidation, anti-aging, whitening, moisturizing and anti-inflammation is insignificant. If the content exceeds 30% by weight, no significant increase in the effect is observed.

The composition of the present invention may be an external preparation for skin or a cosmetic composition.

The term "external preparation" in the present invention refers to a preparation provided for external use, and includes external preparation, external preparation, external preparation, ointment, warning agent and suppository. The external preparation for skin of the present invention, It may be included without limitation, but it may be particularly beneficial.

The composition for external application for skin of the present invention can be used as a composition for external application for skin for antioxidation, which can provide an excellent antioxidative effect by inhibiting the oxidation of DPPH which is an organic radical. In addition, the composition of the present invention can be used as a composition for external application for anti-aging, specifically, for improving wrinkles, which effectively suppresses the expression of collagenase in the skin, thereby reducing collagen decomposition in the skin to improve skin elasticity, An anti-aging effect can be provided.

In addition, the composition of the present invention can be used as a composition for external application for skin whitening, and it can provide an excellent whitening effect by inhibiting the production of melanin. Furthermore, the composition of the present invention can be used as a composition for external application for moisturizing skin, which can enhance the skin barrier function and induce differentiation of dermal keratinocytes. Therefore, it can be effectively used as a composition for external application for skin, which prevents or improves skin dryness or psoriasis caused by incomplete epidermal differentiation.

The external preparation for skin or cosmetic composition of the present invention is an external preparation for skin characterized by antioxidation, anti-aging, skin elasticity improvement or wrinkle improvement, whitening, skin moisturizing or anti-inflammatory, skin antioxidant, anti-aging, whitening, anti- And may be characterized by being free radical scavenging, inhibiting collagenase expression, inhibiting melanin production, inhibiting macrophage release of nitric oxide or differentiating keratinocyte cells, and skin moisturizing effect.

The term "antioxidant" used in the present invention means all mechanisms that lower the degree of oxidative stress. In the present invention, the prevention of skin (cell) damage caused by oxidative stress, Means any mechanism that can improve or treat. Damage due to external causes may include ultraviolet rays, external contaminants, trauma, and the damage caused by internal causes may include inflammation, metabolic stress, and the like.

According to one embodiment of the present invention, in order to confirm the antioxidative effect of ethylhexylgalate, the free radical scavenging ability was analyzed, and as a result, the antioxidative effect of DPPH was suppressed to thereby exhibit an excellent antioxidative effect. Therefore, the ethylhexylgalate of the present invention has an excellent effect on the free radical scavenging ability, so that the composition for external skin application for antioxidants or the cosmetic composition for improving or treating damaged skin (cell) caused by oxidative stress caused by external or internal causes And can be usefully used in compositions.

The term " anti-aging " as used in the present invention means any mechanism that slows or prevents the change of skin cells and tissues upon aging, and the change of skin cells and tissues due to aging is, for example, The decrease in moisture content, and the synthesis of collagen and elastin, which are related to the elasticity of the skin, may be reduced. In the present invention, it may mean the skin elasticity improvement or the wrinkle improvement effect, and preferably the collagenase expression inhibiting effect.

The term "whitening " as used in the present invention means whitening the skin, which means excessive synthesis of melanin or alleviation or improvement of spots, freckles and various pigmentation caused by melanin deposition.

According to one embodiment of the present invention, the melanin formation inhibitory activity of the ethylhexylgalate at the cellular level was confirmed, and as a result, a melanin production inhibitory ability (ethyl (meth) acrylate) similar to that of the whitening agent (hydroquinone, 44.2% Hexylgalate, 47.6%). Therefore, the ethylhexylgalate of the present invention has an excellent melanin synthesis inhibitory activity effect, and has a significant effect on skin whitening, and thus can be usefully used as a skin whitening cosmetic composition or a skin external preparation.

The term "moisturizing" used in the present invention means that the homeostasis of the living body is maintained by suitably controlling water loss (moisture evaporation) and the like. The ethylhexylgalate of the present invention has water retention ability and can be used as a cosmetic composition for skin moisturizing or as an external preparation for skin.

According to one embodiment of the present invention, treatment of ethylhexalgate according to the present invention promoted differentiation in keratinocytes. Therefore, it was found that the ethylhexylgalate according to the present invention enhances the barrier function of the skin and improves the skin moisturizing ability.

The term "anti-inflammatory" as used in the present invention includes all mechanisms for healing inflammation of the skin or inhibiting the inflammatory reaction itself. In the present invention, the inflammatory reaction by macrophages may be inhibited, but not limited thereto.

According to one embodiment of the present invention, it was confirmed that the treatment of ethylhexalgate according to the present invention inhibits the production of NO by LPS, a known skin irritation component. Therefore, it can be seen that the ethylhexylgalate according to the present invention can be effectively used for sensitive skin products by alleviating skin irritation and inflammation.

The cosmetic composition or external preparation for skin of the present invention preferably contains ethylhexyl galeate in an amount of 0.0001 to 30% (w / v) based on the weight of the total composition. If it is contained at less than 0.0001% (w / v), antioxidation, anti-aging, whitening, anti-inflammation and moisturizing effects of the skin can not be substantially expected. On the other hand, when it is contained in excess of 30% (w / v), the overall processability of the composition such as solubility in a solvent is lowered and its use for various purposes such as formulations described later may be restricted.

In addition, the cosmetic composition or external preparation for skin of the present invention may contain ingredients generally accepted in addition to the above-mentioned effective ingredients, and may contain conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes, .

The cosmetic composition or the external preparation for skin of the present invention may be prepared in any formulations conventionally produced in the art, and examples thereof include solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, -Containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and spray, but are not limited thereto. More specifically, it can be manufactured in the form of a flexible lotion (skin), a nutritional lotion (milk lotion), a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder .

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interface-active agent-containing cleansing, the carrier component is selected from the group consisting of aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

In another embodiment, the present invention can provide a quasi-drug composition containing ethylhexyl galeate as an active ingredient. The composition of the present invention may be a quasi-product composition having antioxidant, anti-aging, whitening, anti-inflammation and moisturizing activity of the skin.

When the composition of the present invention is used as an additive, the composition may be added as it is or may be used together with other quasi-drugs or quasi-drugs, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use.

Preferably, the quasi-drug composition may be a disinfectant cleaner, shower foam, gagrin, a wet tissue, a detergent soap, a hand wash, a humidifier filler, a mask, an ointment agent, a coating agent or a filter filler.

In another aspect, the present invention can provide a pharmaceutical composition containing ethylhexgalate as an active ingredient as an effective ingredient. The composition of the present invention may be a pharmaceutical composition having antioxidant, anti-aging, whitening, anti-inflammation and moisturizing activity of the skin, and may be used as an adjuvant for an active ingredient having medicinal use. The pharmaceutical compositions of the present invention may be prepared according to methods well known to those skilled in the art.

The pharmaceutical composition of the present invention may be formulated in the form of tablets, pills, powders, capsules, suspensions, oral solutions, emulsions or syrups, oral preparations, suppositories or injection solutions according to a conventional method have. In detail, when formulated, it can be prepared by using diluents or excipients such as fillers, weighing agents, binders, wetting agents, disintegrants, surfactants and the like which are generally used. Solid formulations for oral administration include, but are not limited to, tablets, pills, powders, granules, capsules, and the like. Such a solid preparation may be prepared by mixing at least one excipient such as starch, calcium carbonate, sucrose, lactose, gelatin and the like in addition to the above composition containing ethylhexyl galeate.

In addition to simple excipients, lubricants such as magnesium stearate and talc may also be used. Liquid preparations for oral administration, liquid paraffin, and various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like. Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like. Examples of the suppository base include withexol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The composition of the present invention may be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) depending on the desired method, and the dose may be determined depending on the condition and weight of the patient, The mode of administration, the route of administration, and the time, but may be suitably selected by those skilled in the art.

The ethylhexogalate according to the present invention has an excellent effect on suppression of collagenase expression, suppression of melanin synthesis, suppression of NO release of macrophage cell line, and increase of water retention ability by inducing keratinocyte differentiation, Anti-aging, whitening, anti-inflammatory and moisturizing cosmetic compositions or external skin preparations.

Hereinafter, the present invention will be described in more detail with reference to the following examples and experimental examples. However, the following examples and experimental examples are provided for illustrating the present invention, and the scope of the present invention is not limited thereto.

Ethyl hexyl gallate of the present invention used in the following examples was synthesized by using ethyl hexanol and gallate using Tannase enzyme and purified and used in the experiment.

Example  1: Antioxidant effect test - DPPH  evaluation

To investigate the antioxidant effect of ethylhexyl galatate, the reduction of 1,1-diphenyl-2-picryl hydrazyl (DPPH) Antioxidant was oxidized). The antioxidant activity was evaluated by comparing the DPPH antioxidant effect with the change of the absorbance. That is, the degree of decrease of the absorbance of the ethylhexylgalate obtained in Example 1 by inhibiting the oxidation of DPPH to that of the control group was measured, and the concentration showing an absorbance of 50% or less as compared with the absorbance of the control group was defined as the effective antioxidant concentration Respectively.

190 μl of 100 μM (in ethanol) DPPH solution and 10 μl of ethylhexylgalate and positive control of Example 1 obtained above were reacted at 37 ° C. for 30 minutes, and the absorbance was measured at 540 nm. Trolox, a synthetic antioxidant widely used as a positive control, was used. The DPPH analysis results of each substance are shown in Table 1 below, and IC ₅₀ means the sample concentration when the absorbance was reduced by 50% by the added sample.

DPPH analysis result Test substance IC ₅₀ (ppm) Trolox (positive control) 43 Ethylhexgalate 150

As can be seen in the above Table 1, the ethylhexylgalate of Example 1 showed similar efficacy effects as compared to Troxox, a synthetic antioxidant used as a positive control. Thus, it was found that the ethylhexylgalate according to the present invention had an excellent antioxidative effect.

Example  2: Wrinkle-improving effect test - Measurement of inhibitory effect on collagenase expression

The ability to inhibit the collagenase production of ethylhexylgalate prepared above was compared with that of tocopherol (Comparative Example 1). Tocopherol is an antioxidant, a substance known to regenerate epidermal cells of the skin and prevent aging of the skin.

Specific experiments were carried out in a 96-well microtiter plate containing 2.5% fetal bovine serum-containing DMEM (Dulbecco's Modified Eagle's Media) to give 5,000 cells / well of human fibroblasts And cultured until it grew to about 90%. After that, the cells were cultured in serum-free DMEM medium for 24 hours, treated with 100 μg / ml of ethylhexogalate of Example 1 dissolved in serum-free DMEM medium and tocopherol at a concentration of 10 moles for 24 hours, Were collected.

The collected cell culture medium was assayed for collagenase production using commercially available collagenase assay kit (Amersham Pharmacia). First, a cell culture solution collected in a 96-well plate uniformly coated with primary collagenase antibody was added and the antigen-antibody reaction was performed in a thermostatic chamber for 3 hours.

After 3 hours, the second collagen antibody bound to the chromophore was placed in a 96-well plate and allowed to react for 15 minutes. After 15 minutes, the color development inducing substance was added and coloration was induced at room temperature for 15 minutes. When the reaction (color development) was stopped by adding 1 M sulfuric acid again, the color of reaction solution was yellow and the degree of yellow was different according to the degree of reaction progress.

The absorbance of a yellow 96-well plate was measured at 405 nm using a spectrophotometer and the degree of synthesis of collagenase was calculated by the following equation. At this time, the reaction absorbance of the cell culture obtained from the untreated group was used as a control. That is, the degree of expression of collagenase in the untreated group was set at 100, and the degree of expression of collagenase in the group treated with the test substance was determined, and the results are shown in Table 2 below.

(%) = (Absorbance of the substance-treated cell group / absorbance of the control group) x 100

Test substance Expression level of collagenase (%) Untreated group 100 Tocopherol (Comparative Example 1) 78 EGCG (Comparative Example 2) 62 Ethylhexgalate 67

The lower the degree of expression of collagenase, the higher the ability to inhibit the expression of collagenase and the less collagen decomposition in the skin, resulting in a smaller amount of wrinkles to be produced. As can be seen from the above Table 2, the ethylhexogalate of the present invention effectively inhibited the expression of collagenase in vitro and confirmed that the expression of collagenase was superior to that of tocopherol known as an antioxidant I could. Thus, it was found that the ethylhexylgalate according to the present invention effectively inhibited the expression of collagenase, thereby reducing the degradation of collagen in the skin, and thus exhibiting an excellent anti-aging effect.

Example  3: Melanin production inhibition test

In order to confirm the whitening effect of ethylhexylgalate prepared at the cell level, ethylhexylgalate and the hydroquinone inhibitory effect of the control group, melanin, were measured and compared.

Specifically, mouse-derived melanoma cells (Dooley, TP et al, Skin pharmacol, 7, pp 188-200) derived from C57BL / 6 mice were cultured in DMEM supplemented with 10% fetal calf serum, 100 nM 12- O-tetradecanoylphorbol-13-acetate and 1 nM cholera toxin at 37 ° C and 5% CO ₂ . The cultured Mel-Ab cells were detached with 0.25% trypsin-EDTA, and the cells were cultured at a concentration of 10 cells / well on a 24-well plate. Then, each test substance was added for 3 days from the second day And cultured.

As a test substance, hydroquinone and ethylhexyl galeate were used at a concentration of 10 ppm, respectively. At this time, the hydroquinone was used as a positive control. Then, the culture solution was removed, washed with PBS (phosphate buffered saline), and the cells were dissolved with 1 N sodium hydroxide. The absorbance was measured at 400 nm, and the inhibition rate of melanin production was calculated according to the following formula. Respectively.

% Inhibition of melanin production = 100- (absorbance of each test substance / control absorbance x 100)

Test substance Melanin production inhibition rate (%) Untreated group 100 Hydroquinone (positive control) 44.2 Ethylhexgalate 47.6

As shown in Table 3, it was confirmed that the ethylhexogalate of the present invention exhibited a degree of inhibition of melanin formation similar to that of hydroquinone, which is a known whitening substance. Therefore, it was found that the ethylhexylgalate according to the present invention effectively inhibits the production of melanin in the skin, thereby excelling in the whitening effect.

Example  4: Analysis of anti-inflammatory effect

The skin anti-inflammatory effect of ethylhexylgalate obtained in Example 1 was measured. The RAW 246.7 macrophage cell line was placed in a DMEM medium containing 10% FBS penicillin (100 IU / ml) and streptomycin (100 μg / ml), and cells were grown in a 37 ° C, 5% CO ₂ incubator. 300 μl was added at a density of several ml / ml, and after 24 hours, the ethylhexylgalate sample was treated at a concentration of 50 ppm. One hour after the sample treatment, 1 μg / ml of LPS was treated. After 18 hours, the amount of NO released into the medium was measured using a Griess reagent. The group treated with dexamethasone was used as a positive control for the anti-inflammatory activity which inhibited the release of NO, and the amount of NO was determined by comparison with the measured value of the standard NaNO ₂ , .

ingredient Concentration (ppm) % Inhibition rate Negative control group 100 LPS 1 ug / ml 0 LPS 1 ug / ml + ethylhexegalate 50 31.1

As shown in Table 4, it was confirmed that the ethylhexogalate of the present invention inhibits the production of NO by LPS, a known skin irritation component. Therefore, it can be seen that the ethylhexylgalate according to the present invention can be effectively used for sensitive skin products by alleviating skin irritation and inflammation.

Example  5: skin moisturizing effect test

In order to confirm the skin barrier function and skin moisturizing ability of ethylhexylgalate, human keratinocytes cultured in a primary culture by the use of absorbance were placed in a culture flask and adhered to the bottom. Then, 5 mg / ml and 10 mg / ml were added to the culture medium and cultured for 5 days until the cells were grown to 80 to 90% of the floor area.

The cultured cells were harvested, washed with PBS (phosphate buffered saline), and then suspended in 10 mM Tris-HCl buffer solution containing 2% SDS (sodium dodecyl sulfate) and 20 mM DTT (Dithiothreitol) 1 ml of Tris-HCl, pH 7.4) was added and sonication was performed for 3 minutes, followed by boiling for 10 minutes. The precipitate was centrifuged at 1200 rpm for 30 minutes, and the precipitate was suspended in 1 ml of PBS and the absorbance at 340 nm was measured.

Separately, a portion of the solution after the sonication was taken to measure the protein content and used as a criterion when evaluating the degree of cell differentiation. The test results are shown in Table 5 below, in which the low calcium (0.03 mM) and high calcium (1.2 mM) treated groups were used as negative / positive control groups and the test substance was added to the low calcium concentration.

Test substance Concentration used The ability to differentiate in keratinocytes (%) Control group Low Ca < ^{2 +} & gt ^; (0.03 mM) 100 Low Ca ²⁺ (1.2 mM) 206 Ethylhexgalate 10 mg / ml 162 5mg / ml 126

As shown in Table 5, when the amount of CE (cornified envelope) produced during differentiation of keratinocytes was measured to compare the promoting effect of cell differentiation, the ethylhexogalate according to the present invention promoted differentiation from keratinocytes . Therefore, it was found that the ethylhexylgalate according to the present invention enhances the barrier function of the skin and improves the skin moisturizing ability.

Example  6: Skin Moisture power  Improve clinical effect

The clinical effects of improving the moisturizing ability of the cosmetic composition containing ethylhexogalate were evaluated in 20 healthy women aged 30 to 45 years old. Dry skin test subjects having skin moisture of 65 or less were selected. The first group was divided into two groups, the group A group was coated with the example, and the group B was coated with the comparative example formulation into the face area twice a day (morning and evening) for 4 weeks. The prescriptions of the examples and comparative examples are shown in Table 6.

ingredient Content (% by weight) Example Comparative Example Ethylhexgalate 0.5 0 Liquid paraffin 5 5 Wax 3 3 Polysorbate-60 2 2 Squall 3 3 glycerin 6 6 Purified water To 100 To 100 Incense, preservative a very small amount a very small amount

Before the start of the experiment, the two parts to be measured were determined, and the moisture content of the skin was measured three times, followed by application (2 times / day) for 4 weeks. The skin moisture content measuring device Corneometer courage & Khazaka, Germany) was used to measure the moisture of the skin layer just below the stratum corneum. The measurement results are summarized in Table 7.

Example Comparative Example D0 D28 D0 D28 Average 43 55 42 49 Increase (%) 27.9 11.4

As shown in Table 7, when the moisture-wettability of the ethylhexalgate-containing example was measured, it was confirmed that the moisturizing power was superior to the comparative example not containing ethylhexgalate. Thus, it was found that ethylhexalgate according to the present invention improves skin moisturization.

The above results indicate that ethylhexylgalate according to the present invention can be used for the purpose of skin-beauty to show various effects. Therefore, it is useful for antioxidation, wrinkle, whitening, anti-inflammation and moisturizing in various fields such as cosmetics, .

From the above description, it will be understood by those skilled in the art to which the present invention pertains that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. In this regard, it should be understood that the above-described embodiments are illustrative in all aspects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, as well as all changes or modifications derived from the meaning and scope of the appended claims and their equivalents.

Claims (7)

Ethylhexogalate as an active ingredient.
The composition for external application for skin according to claim 1, wherein the composition comprises 0.0001% (w / v) to 30% (w / v) ethylhexyl galeate by weight of the total composition.
The composition for external application for skin according to claim 1, wherein the composition is for antioxidation, anti-aging, skin elasticity improvement or wrinkle improvement, whitening, skin moisturizing or anti-inflammatory.
The composition for external application for skin according to claim 1, wherein the composition has free radical scavenging, inhibition of collagenase expression, inhibition of melanin production, suppression of macrophage release of nitric oxide or differentiation of keratinocytes, and skin moisturizing effect .
A cosmetic composition for skin antioxidation, anti-aging, whitening, anti-inflammation or moisturizing comprising ethylhexygalate as an active ingredient.
Ethylhexogalate as an active ingredient.
A pharmaceutical composition comprising ethylhexgalate as an active ingredient.