KR20140131211A - Tube for centrifugation - Google Patents

Abstract

The present invention relates to a container for centrifugation which is characterized by comprising a first space including a space inside; a second space connected with the lower side of the first space and having a space formed inside; a path part connecting the first space and the second space and including a connecting hole where a fluid flows in and out; a first cover part formed to close the upper side of the first space; and the second cover part formed to close the lower side of the second space, wherein the path part is characterized by having the first space and the second space combined to open and close the connecting hole according to the rotation of the second space.

Description

[0002] Tube for centrifugation [

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a centrifuge container, and more particularly, to a centrifuge container improved in structure so that bone marrow or blood separation and PRP concentration can be performed in one container.

Blood consists of plasma, platelets and blood cells, among which platelets contain components necessary for the regeneration and activation of tissue. When blood is centrifuged, it is separated into plasma, platelets and blood cells according to its specific gravity. The separated plasma is separated into platelet rich plasma (PPP) and platelet poor plasma (PPP) . Platelets contain many growth factors and play important roles in wound healing and skin regeneration. It has been reported that PRP contains such platelets at a high concentration, and various growth factors stimulate the proliferation of peripheral cells and stimulate the synthesis of collagen and other components abundantly. Accordingly, PRP has recently been used in various fields such as pain treatment such as back pain, hair loss treatment, and skin diseases such as skin regeneration and burn treatment.

Conventionally, as a method for separating the PRP, a method using two tubular test tubes was used. More specifically, the blood sampled from the human body is injected into a tubular test tube, the injected test tube is subjected to primary centrifugation using a centrifuge, the injector needle is inserted into the tube, and only the plasma located in the uppermost layer is extracted. Then, to obtain high concentration of PRP, the extracted plasma is put into another tube type test tube and subjected to secondary centrifugation. Plasma in the upper layer of the tubular tube is centrifuged and extracted with a syringe to extract remaining PRP. Such a method is disclosed in Korean Patent Laid-Open No. 10-2010-0105282.

However, in this process, erythrocytes can be sucked together in the plasma extraction step after the first centrifugation, and since the Buffy coat layer is formed on the red blood cells in a very small amount, there is a problem that it can not be extracted accurately. There is a problem that it can be exposed to the atmosphere. This problem also occurs when bone marrow is extracted.

Therefore, in order to solve the above-mentioned problems, it is necessary to develop a dedicated kit capable of automatically and accurately separating stem cells and PRPs.

KR 10-2010-0105282 (public number)

An object of the present invention is to provide a centrifuge container capable of minimizing exposure to air during the blood separation process and extracting an object contained in a high concentration.

The present invention provides a display device comprising: a first spatial part having a space formed therein; A second space connected to a lower side of the first space and having a space therein; A passage portion connecting the first space portion and the second space portion and having a communication port through which the fluid enters and exits; A first lid part configured to close an upper side of the first space part; And a second lid part formed to seal the lower side of the second space part; Wherein the passage portion is formed by the first space portion and the second space portion being coupled to each other to open or shut off the communication hole as the second space portion rotates.

The centrifugal separation container according to the present invention is characterized in that the second space portion is formed at one side lower portion of the first space portion, so that the centrifugal separation and concentration work of blood is performed through one container, It is possible to prevent the loss and increase the convenience of the work.

1 is a perspective view of a centrifuge container according to the present invention.
2 is a cross-sectional view of a centrifuge container according to the present invention.
3 is a cross-sectional view illustrating a state in which a communication port of a centrifuge container is shut off according to an embodiment of the present invention.
Fig. 4 is an enlarged cross-sectional view of the passage portion of Fig. 3;
5 is a cross-sectional view illustrating a state in which a communication port of a centrifuge container is opened according to an embodiment of the present invention.
Fig. 6 is an enlarged cross-sectional view of the passage portion of Fig. 5;
7 is an exploded perspective view of a centrifuge container according to the present invention.

The present invention relates to a centrifuge container, comprising: a first space part having a space therein; A second space connected to a lower side of the first space and having a space therein; A passage portion connecting the first space portion and the second space portion and having a communication port through which the fluid enters and exits; A first lid part configured to close an upper side of the first space part; And a second lid part formed to seal the lower side of the second space part; The passage portion is characterized in that the first space portion and the second space portion are coupled and the second space portion rotates to open or shut off the communication hole.

In the present invention, the passage portion is formed to be smaller in diameter than the first space portion and the second space portion.

In addition, the passage portion may include a camera sensor for distinguishing a separation layer of the mixture, and the camera sensor may be a CCD, CMOS, or RGB photosensitive material.

In addition, at least one fixing protrusion may be provided on the outer surface of the first space portion so that the second space portion can be fixed when the second space portion is rotated. At least one rotation protrusion may be provided on the outer surface of the second space portion, . ≪ / RTI >

The first lid portion of the present invention may also include at least one needle penetration portion, and the needle penetration portion may include fluid control means capable of controlling the entry and exit of the fluid.

Meanwhile, at least one gas outlet may be formed on one side of the first lid and the second space of the present invention, and the gas outlet may further include a filter.

In addition, the passage portion and the second lid portion of the present invention may include at least one contact means, and the passage portion may be screwed or unevenly coupled with the first space portion and the second space portion.

Further, the first lid and the second lid of the present invention may be screwed into the first and second spaces.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The sizes and shapes of the components shown in the drawings may be exaggerated for clarity and convenience. In addition, terms defined in consideration of the configuration and operation of the present invention may be changed according to the intention or custom of the user, the operator. Definitions of these terms should be based on the content of this specification.

It is to be understood that within the scope of the appended claims, the invention may be practiced otherwise than as specifically described within the spirit and scope of the appended claims. Of course.

FIG. 1 is a perspective view of a centrifuge container according to the present invention, FIG. 2 is a sectional view of a centrifuge container according to the present invention, and FIG. 3 is a cross- Fig. 5 is a cross-sectional view showing a state in which a communication port of a centrifuge container is opened according to an embodiment of the present invention, Fig. 6 is a cross-sectional view of the passage portion of Fig. 5 Fig. 7 is an exploded perspective view of a centrifuge container according to the present invention. Fig. Hereinafter, the centrifugal separation container of the present invention will be described in detail with reference to FIGS. 1 to 7 and Examples.

The present invention relates to a centrifuge container for separating and concentrating blood as described above.

1 to 7, the centrifugal separation container according to the present invention comprises a first space part having a space formed therein; A second space connected to a lower side of the first space and having a space therein; A passage portion connecting the first space portion and the second space portion and having a communication port through which the fluid enters and exits; A first lid part formed above the first space part; And a second lid part formed to seal the lower side of the second space part; Wherein the passage portion is engaged with the first space portion and the second space portion so as to be rotated so that the communication space can be opened or blocked as the second space portion rotates.

The centrifuge container according to the present invention is a device for extracting only a specific component from a substance having various concentrations of components, and it is possible to use a centrifugal force of a centrifugal separator to separate each component by concentration, Device.

That is, the centrifugal separator according to the present invention is a device for separating components injected into the centrifugal separator by rotating the centrifugal separator at a high speed and separating the components using the centrifugal force.

The container for centrifugation according to the present invention is adapted to extract platelet-rich plasma layer (PRP) from collected blood, but is not limited to specific materials. The present invention can be used not only in platelet-rich plasma layer (PRP) but also in extracting bone marrow, body fluid or cells.

Hereinafter, for the sake of convenience of explanation, it will be described that the mixed substance is limited to blood collected.

The centrifugal separation container according to the present invention is configured such that the blood collected therein is injected into the blood vessel, and the blood components such as red blood cells, platelets, plasma, etc. are divided into layers according to the specific gravity through the centrifugation, The first space portion and the second space portion correspond to the space. In addition, the centrifugal separation container according to the present invention is formed to have a generally cylindrical shape so as to be suitable for a centrifugal separator. The first space part and the second space part according to the present invention may be made of a transparent plastic material so that the state of the inside can be confirmed from the outside.

The first space part is located on the upper side, and the collected blood is accommodated. The blood initially injected into the container is uniformly mixed with each component, so that blood initially injected into the first space is also homogenized.

The second space part is located below the first space part, and the second space part corresponds to a part where red blood cells are located in the blood after centrifugal separation.

The first space part and the second space part include a passage part for connecting the first space part and the second space part. More specifically, the passage portion is engaged with the first space portion and the second space portion so as to rotate, and the communication hole can be opened or blocked as the second space portion rotates. 3 to 6 are enlarged views of a communication part according to an embodiment of the present invention, in which the communication part is closed and opened. Figs. 3 to 4 show a state in which the communication part is closed FIGS. 5 to 6 are views showing a state in which a communication port can be opened by rotating the second space part to allow the fluid to flow in and out. FIG.

In particular, a sealing means for preventing fluid from flowing when the communication port is blocked may be included around the communication port. The sealing means may be a silicone ring commonly used in the art.

The passage portion may be smaller in diameter than the first space portion and the second space portion, and the first space portion and the second space portion may be screwed or unevenly coupled.

Further, the passage portion is characterized by having a camera sensor for precise boundary separation of the mixture. It is possible to accurately extract different components using the camera sensor. At this time, the camera sensor may be CCD, CMOS or RGB photosensitive.

In the meantime, the outer surface of the first space part of the present invention includes at least one fixing protrusion for preventing rotation of the outer space so as to fix the first space part when the second space part is rotated. For example, The projections may be formed to face each other. In addition, the outer surface of the second space portion may include at least one rotation protrusion to be mounted on the rotation means. For example, the rotation protrusion may be formed on the outer surface of the second space portion. In particular, the fixing protrusions and the rotation protrusions may be formed in a rib shape.

1 to 7, the present invention may include a first lid and a second lid to seal the upper side of the first space and the lower side of the second space.

First, the first lid portion may include a needle penetration portion and a gas exhaust port. Here, the needle penetration means a hole through which the needle can pass. In the present invention, it may be a through hole used when injecting blood into the container and extracting the resultant. Further, the needle penetrating portion may include fluid control means for controlling the entry and exit of the fluid, and the fluid control means may be a silicone rubber stopper. Also, the gas discharge port is for discharging air in the first space portion, and the gas discharge port may further include a filter. When the blood is injected into the container and the red blood cells are extracted, the filter allows the microorganisms contained in the outside air to be caught by the filter so that only the air can flow in and out of the first space.

Next, the second lid may comprise at least one sealing means for preventing leakage, which may be a commonly used silicon pad.

Particularly, the first lid part and the second lid part can be screwed to the first space part and the second space part, respectively.

The operation of the present invention having such a configuration will be described below.

First, the anticoagulant is extracted from the vacuum anticoagulant solution tube using a syringe. Here, the term "anticoagulant" means a substance inhibiting blood clotting.

Whole blood is then collected in the syringe containing the anticoagulant, injected into the first space through the needle penetration of the first lid, and centrifuged using a centrifuge.

At this time, the injection amount of blood may be 5 ml to 30 ml, and the communication part of the passage part should be shut off.

When the first centrifugation is completed, the blood contained in the first space is divided into red blood cells, white blood cells, and platelets, and the blood is separated from the upper side in the order of platelets, white blood cells, and red blood cells.

Thereafter, the second space portion is rotated to open the communication port of the passage portion, so that the red blood cell having a large specific gravity is accommodated in the second space portion. At this time, the camera sensor can be used to accurately separate red blood cells. Then, after the red blood cells are injected into the second space, the second space is rotated again to close the communication hole.

Thereafter, secondary centrifugation is carried out using a centrifuge. And the syringe needle is passed through the needle penetration part of the secondary centrifugation complete drawing, and the platelet-rich plasma layer (PRP) is extracted to the outside.

Accordingly, the centrifuge separation container of the present invention is capable of separating and concentrating blood through centrifugal separation through a single container, thereby preventing loss occurring in the conventional transfer process and improving the convenience of operation have.

The embodiments described above are intended to illustrate the contents of the present invention and are not intended to limit the scope of the present invention. Embodiments of the present invention are provided to more fully describe the present invention to those skilled in the art.

100:
110: first cover part 120: fixing protrusion
130: needle penetration part 131: fluid control part
140, 140: gas outlet 141, 141: filter
200: second space portion
210: second cover part 220: rotation protrusion
230: Sealing means
300: passage portion 310:

Claims (13)

A first space part having a space formed therein;
A second space connected to a lower side of the first space and having a space therein;
A passage portion connecting the first space portion and the second space portion and having a communication port through which the fluid enters and exits;
A first lid part configured to close an upper side of the first space part; And
A second lid part formed to seal the lower side of the second space part; / RTI >
Wherein the passage portion is formed by the first space portion and the second space portion being coupled to each other to open or close the communication hole as the second space portion rotates. The method according to claim 1,
Wherein the passage portion is smaller in diameter than the first space portion and the second space portion. The method according to claim 1,
Wherein said passageway comprises a camera sensor for separating the separation layer of the mixture. The method of claim 3,
Wherein the camera sensor is CCD, CMOS, or RGB photosensitive. The method according to claim 1,
Wherein at least one fixing protrusion is provided on an outer surface of the first space part so that the second space part can be fixed when the second space part is rotated. The method according to claim 1,
Wherein at least one rotation protrusion is provided on the outer surface of the second space part so as to be mounted on the rotation means. The method according to claim 1,
Wherein the first lid includes at least one needle penetration. 8. The method of claim 7,
Wherein the needle penetrating portion includes fluid control means capable of controlling the entry and exit of the fluid. The method according to claim 1,
And at least one gas outlet is formed on one side of the first lid part and the second space part. 10. The method of claim 9,
Wherein said gas outlet further comprises a filter. The method according to claim 1,
Wherein said passage portion and said second lid portion comprise at least one contact means. The method according to claim 1,
Wherein the passage portion has a first space portion and a second space portion that are screwed or unevenly coupled. The method according to claim 1,
Wherein the first lid part and the second lid part are screwed to the first space part and the second space part.

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