KR20140092094A - Mixture Compostion of Food for promoting collagen synthesis and improving skin moisture - Google Patents
Mixture Compostion of Food for promoting collagen synthesis and improving skin moisture Download PDFInfo
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- KR20140092094A KR20140092094A KR1020130004401A KR20130004401A KR20140092094A KR 20140092094 A KR20140092094 A KR 20140092094A KR 1020130004401 A KR1020130004401 A KR 1020130004401A KR 20130004401 A KR20130004401 A KR 20130004401A KR 20140092094 A KR20140092094 A KR 20140092094A
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- South Korea
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- collagen
- weight
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- food
- peptide
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/39—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
본 발명은 제과류, 제빵류, 튀김류, 면류 또는 떡류의 곡류 식품에 다양하게 활용될 수 있는 식품 조성물로서, 자세하게는 곡물에 더하여 콜라겐 효소분해 펩타이드와 히알루론산을 일정 함량으로 포함함으로써, 콜라겐의 체내 합성을 촉진하고 피부 보습 효과가 있는 기능성 식품 조성물에 대한 것이다.
The present invention relates to a food composition which can be used variously in confectioneries, bakery products, fried foods, noodles or rice cakes. More specifically, it comprises collagen enzyme degrading peptide and hyaluronic acid in addition to grain, And a skin moisturizing effect.
피부노화는 노화에 미치는 요인에 따라 내인성 노화와 외인성 노화로 구분할 수 있는데, 내인성 노화는 나이가 들어감에 따라 피부의 구조와 생리기능이 환경변화와는 무관하게 감퇴하는 것이고, 외인성 노화는 태양광선 등의 외부환경에 지속적으로 노출되기 때문에 나타나는 것이다. Skin aging can be classified into endogenous aging and extrinsic aging depending on the factors that affect aging. The aging of the endogenous aging is accompanied by a decrease in the structure and physiological functions of the skin irrespective of changes in the environment. Of the total population.
피부노화가 진행되면 표피, 진피 등의 구조 및 기능적인 변화로 인해 피부가 탄력성을 잃고 처져 보이게 된다. 진피의 두께는 감소하는데 반해 콜라겐(교원질)의 총량은 성인이 된 후 1년에 1%씩 소실되며, 남아있는 콜라겐 섬유는 점차 두꺼워지면서 가교가 증가하게 되어 용해도, 팽창력 등이 감소한다. 아울러 탄력섬유도 두꺼워지게 되며, 가교도 증가하게 된다. 그 외 진피 내에서는 섬유아세포의 증식 활성이 떨어지고 콜라겐의 합성, 분해능 역시 감소한다.As the skin ages, the skin becomes unstable due to the structural and functional changes of the epidermis and dermis. While the thickness of the dermis decreases, the total amount of collagen (collagen) disappears 1% per year after becoming an adult. The remaining collagen fibers gradually thicken and increase cross-linking, resulting in decreased solubility and swellability. In addition, the elastic fibers are thickened, and the crosslinking is also increased. In other dermis, the proliferation activity of fibroblasts is decreased, and the synthesis and resolution of collagen are also decreased.
한편, 콜라겐은 피부노화와 관련된 주요 피부조직 성분이며, 지방을 제외한 피부 전체 건조 중량의 77%, 진피섬유 성분의 90%를 차지하고 있는 단백질로서 피부의 강도, 탄력성, 유연성을 유지할 수 있게 하는 기능을 부여한다. 따라서, 콜라겐의 합성증진 및 분해억제는 피부미용 및 노화억제와 관련하여 주요 관심사가 되었으며, UV에 의한 광노화를 억제하면서 콜라겐의 합성을 증진시킬 수 있는 미용식품의 개발이 요구되고 있다.On the other hand, collagen is a major skin tissue component related to skin aging. It is a protein that occupies 77% of the dry weight of the skin except the fat and 90% of the dermis fiber component. It functions to maintain the strength, elasticity and flexibility of the skin. . Therefore, the promotion of collagen synthesis and inhibition of decomposition has been a major concern in relation to skin cosmetic and anti-aging inhibition, and it is required to develop a cosmetic food that can enhance the synthesis of collagen while suppressing photoaging due to UV.
이를 위하여 경구로 섭취할 수 있는 콜라겐에 대한 연구가 지속적으로 이루어져, 다양한 물질로부터 추출되고, 다양한 후처리 과정을 거친 콜라겐 원료들이 개발되었다. 이와 관련하여 국내공개특허공보 제2001-0075842호에는 콜라겐이 함유된 기능성 식품이 개시되어 있고, 국내공개특허공보 제2002-0085307호에는 “경구용 피부미용 식품 조성물”로서 콜라겐이 다량 함유된 조성물이 개시되어 있다. For this purpose, studies on collagen that can be ingested orally have been continuously carried out, and collagen materials that have been extracted from various substances and subjected to various post-treatment processes have been developed. Korean Patent Laid-Open Publication No. 2001-0075842 discloses a functional food containing collagen, and Korean Patent Publication No. 2002-0085307 discloses a composition containing a large amount of collagen as " oral skin cosmetic food composition "Lt; / RTI >
그러나, 상기와 같은 콜라겐 자체는 열에 대한 안정성이 떨어질 뿐만 아니라 분자량이 크므로 다양한 음식물에 적용하기 어려웠으며, 소화 흡수의 용이성, 유효량의 체내 도달 여부 등 다양한 문제점이 있어 식품 분야로의 실질적인 적용에 어려움이 있었다.
However, since collagen itself has poor stability against heat and has a large molecular weight, it is difficult to apply to various foods, and there are various problems such as ease of digestion and absorption, .
상기와 같은 문제점을 해결하기 위하여, 본 발명은 콜라겐의 체내 합성을 촉진하고 피부 보습 효과를 증진시킬 수 있는 기능성 식품 조성물을 제공하는 것을 목적으로 한다.In order to solve the above problems, it is an object of the present invention to provide a functional food composition capable of promoting the synthesis of collagen in the body and enhancing skin moisturizing effect.
또한, 본 발명은 열적 안정성 및 체내 흡수성을 높여 제과류, 제빵류, 튀김류, 면류 또는 떡류 등의 다양한 곡류 식품에 적용시킬 수 있어 일상생활에서 손쉽게 섭취할 수 있으며, 맛과 식감 또한 향상시킬 수 있는 기능성 식품 조성물을 제공하는 것을 목적으로 한다.
Further, the present invention can be applied to a variety of cereal foods such as confectionery, bakery products, fried foods, noodles or rice cakes by enhancing thermal stability and absorbability in the body, so that they can be easily ingested in daily life, It is an object of the present invention to provide a food composition.
상기 목적을 달성하기 위하여 본 발명은 제과류, 제빵류, 튀김류, 면류 또는 떡류의 곡류 식품에 사용되며, 콜라겐의 체내 합성을 촉진하고 피부 보습 효과가 있는 식품 조성물로서, 곡물 1000 중량부에 대하여 i) 콜라겐 효소분해 펩타이드 분말 5~7 중량%, 및 ⅱ) 히알루론산 1~3 중량%을 포함하는 식품 조성물을 제공한다. 이때, 상기 콜라겐 효소분해 펩타이드는 체내에서 효율적으로 섬유아세포와 상호작용할 수 있도록, 평균 분자량이 1000 Da 이하인 것이 바람직하다.In order to achieve the above object, the present invention provides a food composition which is used for a cereal food of a confectionery, a bakery, a fried food, a noodle or a rice cake and promotes the synthesis of collagen and has a skin moisturizing effect. 5 to 7% by weight of a collagen enzyme digesting peptide powder, and ii) 1 to 3% by weight of hyaluronic acid. At this time, it is preferable that the collagen enzyme degrading peptide has an average molecular weight of 1000 Da or less so that it can efficiently interact with fibroblasts in the body.
또한, 상기 콜라겐 효소분해 펩타이드 분말의 열적 안정성을 더욱 향상시키기 위하여, 상기 분말을 코팅액으로 코팅하여 마이크로캡슐화할 수 있는데, 이 경우 상기 코팅액은 쉘락(Shellac), 제인-디피(Zein-DP) 또는 HPMC(hydroxypropyl methylcellulose) 중 어느 하나를 포함하고, 상기 코팅이 분무속도 0.5~1.0㎖/min, 공기 유입온도 60 내지 80℃ 및 분무압력 1.5 내지 2.5 ㎏/㎠의 유동층(Fluid Bed) 반응기에서 수행되는 것이 바람직하다. 그리고, 코팅 효율을 높이기 위하여, 상기 펩타이드 분말과 코팅액의 비율이 1:1~2인 것이 바람직하다.In order to further improve the thermal stability of the collagen enzyme degrading peptide powder, the powder may be coated with a coating solution to be microencapsulated. In this case, the coating solution may be a solution of Shellac, Zein-DP or HPMC (hydroxypropyl methylcellulose), and the coating is carried out in a fluid bed reactor having a spraying rate of 0.5 to 1.0 ml / min, an air inlet temperature of 60 to 80 ° C and a spray pressure of 1.5 to 2.5 kg / cm 2 desirable. In order to increase the coating efficiency, the ratio of the peptide powder to the coating solution is preferably 1: 1 to 2.
한편, 콜라겐의 체내 합성을 촉진하기 위하여, 글루타민 펩타이드를 함유하는 단백질 가수분해물 1~6중량%를 추가로 포함하거나 비타민C 1~2중량% 및 칼슘 1~2중량%를 추가로 포함할 수 있다.
On the other hand, in order to promote the synthesis of collagen in the body, it may further comprise 1 to 6% by weight of protein hydrolyzate containing glutamine peptide, or may further include 1 to 2% by weight of vitamin C and 1 to 2% by weight of calcium .
본 발명의 기능성 식품 조성물은 콜라겐의 체내 합성을 촉진하고 피부 보습 효과를 증진시킬 뿐만 아니라, 열적안정성 및 체내 흡수성을 높여 제과류, 제빵류, 튀김류, 면류 또는 떡류 등의 다양한 곡류 식품에 적용시킬 수 있어 일상생활에서 손쉽게 섭취할 수 있다. The functional food composition of the present invention can promote the synthesis of collagen in the body and enhance the skin moisturizing effect and increase the thermal stability and the absorbability of the body to be applied to a variety of cereal foods such as confectionery, baked goods, fried foods, noodles or rice cakes It can be easily ingested in daily life.
또한, 식품의 맛과 식감을 향상시키며, 제조 후 일정시간이 경과하여도 부드럽고 쫄깃한 맛과 식감이 오래 지속되고, 굳은 상태의 것을 다시 열처리하여도 본래의 맛과 식감이 그대로 나타날 수 있다.
In addition, it improves the taste and texture of foods, and the soft and chewy taste and texture are maintained for a long time even after a certain period of time after manufacture, and the original taste and texture can be displayed even if the hardened state is heat-treated again.
도 1은 시료에 따른 피부섬유아세포의 콜라겐 합성량을 비교한 그래프이다.1 is a graph comparing collagen synthesis amounts of dermal fibroblasts according to a sample.
이하, 본 발명의 콜라겐의 체내 합성을 촉진하고 피부 보습 효과가 있는 식품 조성물을 바람직한 실시예들을 참조하여 상세히 설명하기로 한다.Hereinafter, a food composition which promotes the in vivo synthesis of collagen of the present invention and has a skin moisturizing effect will be described in detail with reference to preferred embodiments.
본 발명의 식품조성물은 제과류, 제빵류, 튀김류, 면류 또는 떡류의 곡류 식품에 사용되는 식품조성물로서, 콜라겐의 체내 합성을 촉진하고 피부 보습 효과를 주기 위하여, 곡물 1000 중량부에 대하여 i) 콜라겐 효소분해 펩타이드 분말 5~7 중량%, 및 ⅱ) 히알루론산 1~3 중량%를 포함하는 것을 특징으로 한다.
The food composition of the present invention is a food composition for use in cereals of confectionery, bakery, fried, noodle or rice cakes. In order to promote the synthesis of collagen in the body and to impart skin moisturizing effect, 5 to 7% by weight of a disintegrating peptide powder, and ii) 1 to 3% by weight of hyaluronic acid.
콜라겐은 인체 내에서 양적, 기능적으로 가장 큰 부분을 차지하는 단백질로, 인체 내 모든 세포의 뼈대를 형성하는 세포외기질, 구체적으로는 결합조직의 주요 구성 인자를 의미하며, 상기 결합조직의 노화로 인해 피부 주름 및 처짐, 골다공증 등의 인체 노화 질환들이 비롯되는 것으로 알려져 있다. Collagen is a protein that occupies the largest quantitative and functional part in the human body. It refers to an extracellular matrix that forms a skeleton of all cells in the human body, specifically, a major constituent of connective tissue. Due to aging of the connective tissue Skin wrinkles and sagging, and osteoporosis.
한편, 섬유아세포는 결합조직의 중요한 성분을 이루는 세포로서 콜라겐 등 조직성분을 합성하며, 외인성 또는 내인성 요인들로 인하여 상기 섬유아세포의 생리 활성 기능이 저하될 경우, 피부 조직 내 콜라겐의 양이 감소하고 이에 따라 세포의 생리 활성 기능이 감소하는 악순환의 원인이 된다. 따라서 섬유아세포의 콜라겐 합성은 노화, 주름 형성 및 손상된 조직의 재생과 직접적인 관련이 있다고 볼 수 있다.On the other hand, fibroblasts are cells constituting an important component of connective tissues and synthesize tissue components such as collagen. When the physiological activity of the fibroblasts is lowered due to extrinsic or intrinsic factors, the amount of collagen in the skin tissue decreases Thus causing a vicious cycle in which the physiological activity of cells is reduced. Thus, collagen synthesis in fibroblasts is directly related to aging, wrinkling, and regeneration of damaged tissue.
종래에는 콜라겐을 섭취하기 위하여, 콜라겐이 다량 함유된 음식을 섭취하거나, 순수 콜라겐을 추출하여 이를 음료수나 정제된 형태로 섭취하였다. 그러나, 상기 콜라겐은 열에 매우 불안정할 뿐 아니라 고유의 특이한 맛으로 인하여 일반적인 식품의 재료로서 사용되는 데에는 한계가 있었다. 또한, 소화 흡수의 용이성, 유효량의 체내 도달 여부 등 다양한 문제점이 있어 식품 분야로의 실질적인 적용에도 어려움이 있었다. Conventionally, in order to consume collagen, food containing a large amount of collagen is ingested, or pure collagen is extracted and taken in a drink or purified form. However, the collagen is not only unstable to heat, but also has a limitation in being used as a general food material because of its unique peculiar taste. In addition, there are various problems such as ease of digestion and absorption, an effective amount reaching the inside of the body, and the like, and thus it has been difficult to apply the composition to a food field.
이에, 본 발명에서는 식품으로의 적용성 및 소화 흡수성을 높이기 위하여 콜라겐을 효소 처리하여 가공한 콜라겐 펩타이드 분말을 사용하며, 상기 폴라겐 펩타이드가 체내에서 섬유아세포의 콜라겐 합성을 촉진함으로써, 결과적으로 피부보습효과를 높이는 것을 특징으로 한다.Therefore, in the present invention, collagen peptide powder processed by enzymatic treatment of collagen is used in order to improve applicability to food and digestion absorbability, and the polar peptide promotes the collagen synthesis of fibroblasts in the body, And the effect is enhanced.
이때, 상기 콜라겐 효소분해 펩타이드는 체내에서 효율적으로 섬유아세포와 상호작용할 수 있도록, 평균 분자량이 1000 Da 이하인 것이 바람직하며, Gly-X-Y 형태의 트리펩티드를 15~95%를 함유한 복합물로서, 이때 X 및 Y는 어떠한 아미노산도 가능하다. 또한 상기 분해효소로는 펩틴 또는 콜라게나제 등이 사용될 수 있다. At this time, the collagen enzyme-degrading peptide preferably has an average molecular weight of 1000 Da or less so as to efficiently interact with fibroblasts in the body, and is a complex containing 15 to 95% of a tripeptide of Gly-XY form, wherein X And Y can be any amino acid. As the above-mentioned degrading enzyme, peptin or collagenase may be used.
또한, 본 발명의 콜라겐 효소분해 펩타이드 분말의 함량은 곡물 1000 중량부에 대하여 5~7 중량%이 사용되는 것이 바람직하며, 이보다 적은 경우 펩타이드 분말 첨가에 따른 효과를 얻기 어려우며, 이보다 많은 경우 효과에 비하여 비용이 과다하게 소요되고, 식품 자체의 맛과 식감에 영향을 줄 수 있다.
In addition, the content of the collagen enzyme degrading peptide powder of the present invention is preferably 5 to 7% by weight based on 1000 parts by weight of the cereal. When the amount is less than that, it is difficult to obtain the effect of addition of the peptide powder. It is expensive and can affect the taste and texture of the food itself.
또한, 본 발명의 식품 첨가물은 상기 콜라겐 효소분해 펩타이드와 함께 히알루론산 1~3 중량%를 포함하는 것을 특징으로 한다. 상기 히알루론산은 아미노산과 우론산으로 이루어지는 복잡한 다당류의 하나로 눈의 초자체나 탯줄 등에 존재하는데, 세균의 침입이나 독물의 침투를 막는 역할을 한다. 근래에는 연쇄상 구균의 발효로 대량 생산하여 화장품과 의약품 분야에서 널리 사용되고 있다.In addition, the food additive of the present invention is characterized by comprising 1 to 3% by weight of hyaluronic acid together with the collagen enzyme degrading peptide. The hyaluronic acid is a complex polysaccharide composed of amino acid and uronic acid, and exists in the vitreous body of the eye and the umbilical cord, and plays a role of preventing invasion of bacteria or penetration of poison. In recent years, fermentation of streptococci has been mass-produced and widely used in cosmetics and pharmaceuticals.
상기 히알루론산은 본 발명의 콜라겐 펩타이드 분말과 함께 작용하여 피부 내 콜라겐의 합성을 촉진시키고, 광노화를 억제시킬 뿐만 아니라 피부 보습을 증가시키고, 콜라겐 펩티드의 생체 잔존율을 높이는 등 전반적으로 피부 상태를 개선시킬 수 있다.The hyaluronic acid acts together with the collagen peptide powder of the present invention to promote the synthesis of collagen in the skin, to suppress the photoaging, to increase the skin moisturization, to increase the survival rate of the collagen peptide, .
또한, 본 발명의 히알루론산의 함량은 곡물 1000 중량부에 대하여 1~3 중량%이 사용되는 것이 바람직하며, 이보다 적은 경우 콜라겐 체내합성 촉진 효과를 얻기 어려우며, 이보다 많은 경우 효과에 비하여 비용이 과다하게 소요되고, 식품 자체의 맛과 식감에 영향을 줄 수 있다.
In addition, the content of hyaluronic acid of the present invention is preferably 1 to 3% by weight based on 1000 parts by weight of the cereal, and when it is less than that, it is difficult to obtain the effect of promoting the synthesis of the collagen body. And can affect the taste and texture of the food itself.
한편, 상기 콜라겐 효소분해 펩타이드 분말의 열적 안정성을 더욱 향상시키기 위하여, 상기 분말을 코팅액으로 코팅하여 마이크로캡슐화할 수 있는데, 이 경우 상기 코팅액은 쉘락(Shellac), 제인-디피(Zein-DP) 또는 HPMC(hydroxypropyl methylcellulose) 중 어느 하나가 사용될 수 있으나, 이에 제한되는 것은 아니며 당업계에 통상적으로 사용되는 코팅소재를 사용할 수도 있다. 본 발명에 따른 코팅소재는 상기 펩타이드 분말에 대하여 1~3배, 바람직하게는 2배 중량으로 사용할 수 있다 .In order to further improve the thermal stability of the collagen enzyme degrading peptide powder, the powder may be coated with a coating solution to be microencapsulated. In this case, the coating solution may be prepared by dissolving or dispersing the collagen enzyme degrading peptide powder in a solvent such as Shellac, Zein- (hydroxypropyl methylcellulose) may be used. However, the present invention is not limited thereto, and coating materials conventionally used in the art may be used. The coating material according to the present invention may be used at a weight of 1 to 3 times, preferably 2 times, relative to the peptide powder.
이때, 상기 캡슐의 입자크기를 최소화하며 코팅효율의 최대화하기 위하여, 유동층공정기를 사용하는 것이 바람직하며, 상기 유동층공정기는 분무속도 0.5~1.0 mL/min, 바람직하게는 0.7 mL/min, 공기 유입온도 60~80℃, 바람직하게는 70℃, 및 분무압력 1.5~2.5 ㎏/㎠, 바람직하게는 1.9 ㎏/㎠의 조건으로 설정한다.
At this time, it is preferable to use a fluidized bed process unit in order to minimize the particle size of the capsule and maximize the coating efficiency, and the fluidized bed process unit has a spraying rate of 0.5 to 1.0 mL / min, preferably 0.7 mL / 60 to 80 占 폚, preferably 70 占 폚, and a spraying pressure of 1.5 to 2.5 kg / cm2, preferably 1.9 kg / cm2.
한편, 본 발명의 식품 첨가물은 콜라겐의 체내 합성을 촉진하기 위하여, 글루타민 1~6중량%를 추가로 포함하거나 비타민C 1~2 중량% 및 칼슘 1~2 중량%를 추가로 포함할 수 있다. Meanwhile, the food additive of the present invention may further comprise 1 to 6% by weight of glutamine, 1 to 2% by weight of vitamin C and 1 to 2% by weight of calcium to promote the synthesis of collagen in the body.
일반적으로, 피부섬유아세포의 콜라겐 합성 기작은 크게 1) 콜라겐 유전자의 전사 시작, 2) 전사(mRNA합성) 3) 번역(단백질 합성), 4) 번역 후 변형, 5) 분비 및 추가 변형의 5 단계의 단백질 유전자 발현 과정을 기본 틀로 한다. 이를 고려할 때, 단일 물질 투여만으로 인체의 가장 큰 조직인 피부의 콜라겐 합성 촉진을 유도하는 것에는 한계가 있으므로, 콜라겐 합성을 촉진하는 단백질 가수분해물 및 비타민과 미네랄을 함께 사용하는 것이 바람직하다.In general, the collagen synthesis mechanism of dermal fibroblasts is largely classified into two stages: 1) initiation of transcription of collagen gene, 2) transcription (mRNA synthesis), 3) translation (protein synthesis), 4) post-translational modification, The protein gene expression process. Considering this, it is preferable to use a protein hydrolyzate and vitamins and minerals that promote collagen synthesis, since there is a limit to induce collagen synthesis promotion of the skin, which is the largest tissue of the human body, only by administering a single substance.
상기 단백질 가수 분해물은 단백질에 산 또는 염기 처리, 가열 처리, 효소 처리 등을 하여 가공한 단백질 유래 조성물을 포함하며, 바람직하게는 콜라겐 합성 증대 효과가 있으며, 사람 또는 동물의 상처 부위에서의 콜라겐 합성을 증가시키는 L-글루타민을 포함하는 글루타민 및 글루타민 펩타이드를 포함한다. 이때, 상기 단백질 가수 분해물은 체내에서 실질적으로 섬유아세포와 상호 작용하기 위해서 1000 Da 이하의 저분자량을 가지는 것이 바람직하다.The protein hydrolyzate includes a protein-derived composition prepared by treating the protein with an acid or a base, a heat treatment, an enzyme treatment, etc. Preferably, the protein hydrolyzate has an effect of increasing collagen synthesis. Glutamine and glutamine peptides, including L-glutamine. At this time, it is preferable that the protein hydrolyzate has a low molecular weight of 1000 Da or less in order to substantially interact with fibroblasts in the body.
한편, 본 발명의 식품 첨가물은 콜라겐의 체내 합성을 촉진하기 위하여 비타민을 포함하며, 구체적으로 상기 비타민은 비타민 C인 것이 바람직하다. 또한, 본 발명의 식품 첨가물은 콜라겐의 체내 합성을 촉진하기 위하여 미네랄을 포함하며, 상기 미네랄은 칼슘, 인, 칼륨, 나트륨, 염소, 마그네슘, 철, 요오드, 구리, 아연, 코발트, 망간 이온 등 생물체를 구성하는 원소 중에서 탄소, 수소, 산소를 제외한 생물체의 무기적 구성요소를 의미한다. 이때, 콜라겐의 효율적인 체내 합성을 위하여 상기 미네랄은 칼슘 이온(Ca2 +)인 것이 바람직하다. Meanwhile, the food additive of the present invention includes vitamins to promote the synthesis of collagen in the body. Specifically, the vitamin is preferably vitamin C. The food additive of the present invention includes minerals for promoting the synthesis of collagen in the body and the minerals are selected from the group consisting of calcium, phosphorus, potassium, sodium, chlorine, magnesium, iron, iodine, copper, zinc, cobalt, Means inorganic components of an organism other than carbon, hydrogen, and oxygen. At this time, it is preferable that the mineral is calcium ion (Ca < 2 + & gt ; ) in order to efficiently synthesize collagen efficiently.
또한, 상기 글루타민의 함량은 곡물 1000 중량부에 대하여 1~6중량%를 추가로 포함되고, 상기 비타민C 및 칼슘의 함량은 곡물 1000 중량부에 대하여 1~3 중량%인 것이 바람직하며, 이보다 적은 경우 콜라겐 체내합성 촉진 효과를 얻기 어려우며, 이보다 많은 경우 효과에 비하여 비용이 과다하게 소요되고, 식품 자체의 맛에 영향을 줄 수 있다.In addition, the content of glutamine is 1 to 6% by weight with respect to 1000 parts by weight of cereals, and the content of vitamin C and calcium is preferably 1 to 3% by weight with respect to 1000 parts by weight of cereals, It is difficult to obtain the effect of promoting collagen synthesis, and in many cases, it is more expensive than the effect and may affect the taste of the food itself.
이하, 실시예, 비교예 및 실험예를 들어 본 발명의 구성 및 효과를 보다 구체적으로 설명한다. 그러나 이들 실시예, 비교예 및 실험예는 본 발명에 대한 이해를 돕기 위해 예시의 목적으로만 제공된 것일 뿐 본 발명의 범주 및 범위가 하기 실시예, 비교예 및 실험예에 의해 제한되는 것은 아니다.
Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to Examples, Comparative Examples and Experimental Examples. However, these examples, comparative examples, and experimental examples are provided for illustrative purposes only in order to facilitate understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples, comparative examples and experimental examples.
[1] 콜라겐 펩타이드 분말의 제조[1] Preparation of Collagen Peptide Powder
돼지의 피부로부터 저분자 올리고 펩타이드 함량이 높은 콜라겐 펩타이드 조성물을 제조하였다. 반응기에 투입된 원재료를 염기(1N NaOH, Na2SO4 및 NaHCO3) 및 산(1NHCl과 H2SO4)으로 처리하고, 상압 가열을 통한 살균 공정으로 전처리하였다. 프로테아제와 펩티다아제로 구성된 가수분해효소를 전체 중량당 2 중량%로 처리한 후, 탈색, 탈취하고, 정제 과정을 순차적으로 거쳐 평균 분자량이 약 800Da 이하인 콜라겐 펩타이드 분말을 제조하였다. A collagen peptide composition having a high molecular oligopeptide content from the skin of pigs was prepared. The raw materials charged into the reactor were treated with bases (1N NaOH, Na2SO4 and NaHCO3) and acids (1NHCl and H2SO4) and pretreated with sterilization process by heating at atmospheric pressure. The collagen peptide powder having an average molecular weight of about 800 Da or less was prepared by treating 2% by weight of hydrolytic enzyme consisting of protease and peptidase, decoloring, deodorizing, and purifying sequentially.
[2] 콜라겐 펩타이드 분말의 코팅[2] coating of collagen peptide powder
상기 실시예1에서 제조한 분말을 유동층공정기(Miniglatt, Glatt, Germany)를 사용하여 마이크로캡슐을 제조하였다. 유동층공정기에 제조된 추출물 분말 50 g을 넣고 예비건조를 위해 유입공기온도 60℃, 유출공기온도 40℃, 공정온도 50℃에서 30분간 추출물 분말의 예비건조를 실시한 후, 유동화압력 0.4 ㎏/㎠에서 각각의 제조공정별로 코팅액을 분무시켜 캡슐화하였다. 이때 코팅액은 쉘락(Shellrak), 10% 제인-디피(제인-디피; in 80% EtOH)을 사용하였으며, 분말과 코팅액의 비율은 1:2(g/g)로 하였다. The powder prepared in Example 1 was microcapsulated using a fluidized bed processer (Minigltt, Glatt, Germany). After 50 g of the extract powder prepared in the fluidized bed process was preliminarily dried for 30 minutes at an inlet air temperature of 60 ° C, an outlet air temperature of 40 ° C and a process temperature of 50 ° C for preliminary drying, the extract powder was preliminarily dried at a fluidization pressure of 0.4 kg / The coating solution was sprayed for each manufacturing process and encapsulated. At this time, the coating liquid was a Shellrak, 10% Jane-Dipi (in 80% EtOH), and the ratio of the powder to the coating liquid was 1: 2 (g / g).
[3] 히아루롤산과의 혼합 조성물[3] Mixture composition with hyaluronic acid
상기 코팅된 콜라겐 펩타이드 분말과 히아루롤산 25g을 혼합하여 실시예1을 제조하였다. Example 1 was prepared by mixing 25 g of the coated collagen peptide powder and hyaluronic acid.
[4] 글루타민 펩타이드와의 혼합 조성물 [4] Mixture composition with glutamine peptide
글루타민 함량이 높은 밀 글루텐을 원재료로 사용하여, 상기 [1]의 콜라겐 펩타이드와 동일한 방법으로 평균 분자량 500-1000 Da의 저분자 글루타민 펩타이드 25g을 제조하여 실시예 1과 혼합하여 실시예 2를 제조하였다. 25 g of a low molecular weight glutamine peptide having an average molecular weight of 500-1000 Da was prepared in the same manner as the collagen peptide of [1], using wheat gluten having a high glutamine content as a raw material, and was mixed with Example 1 to prepare Example 2.
[5] 비타민 C 와 칼슘과의 혼합 조성물 [5] Mixed composition of vitamin C and calcium
비타민 C와 칼슘 분말 20g을 상기 실시예2와 혼합하여 입하여 각각 실시예 3로 하였다.Vitamin C and 20 g of calcium powder were mixed with the above-mentioned Example 2 to prepare the same as Example 3, respectively.
[6] 피부 섬유 아세포의 콜라겐 합성 촉진 효과 평가[6] Evaluation of promoting collagen synthesis in skin fibroblasts
사람 피부섬유아세포를 10% Fetal Bovine Serum과 1%의 페니실린과 스트렙토마이신이 첨가된 Dulbeccos's Minimum Essential Medium에 배양하여 계대수 5 이하를 유지하며, 상기 실시예 처리에 따른 콜라겐 합성량의 변화를 생체 외(in vitro 상)에서 실험하여, 사람 피부섬유아세포의 콜라겐 합성 촉진 효과를 평가하였다. 또한 비교예로서 무처리된 DMEM 배지를 준비하여 비교하였다. Human skin fibroblasts were cultured in Dulbeccos's Minimum Essential Medium supplemented with 10% Fetal Bovine Serum and 1% penicillin and streptomycin to maintain the number of passages below 5, and the amount of collagen synthesis according to the above- (in vitro) to evaluate the promoting effect of human skin fibroblasts on collagen synthesis. As a comparative example, untreated DMEM medium was prepared and compared.
시료별로 사람 피부섬유아세포를 웰 플레이트(well plate)에 1x 104cells/㎠의 밀도로 seeding한 후 세포 부착과 환경 적응을 위해 24시간 동안 인큐베이터(incubator)(37°C, 5% CO2)에서 배양하였다. 그리고 배지를 제거한 후, 혈청(Serum)이 첨가되지 않은 배지를 처리하여 8시간 동안 starvation시켰다. 상기 실시예 조성물을 배지에 용해하여 시료별로 처리하였다. 시료 처리 후, 사람 피부 섬유 아세포를 72시간 동안 배양하고 배지의 상층액을 취하여 합성된 콜라겐을 정량하였다. 콜라겐 정량은 타입 1 프로콜라겐 C-펩타이드EIA 키트(Type I Procollagen C-Peptide EIA kit)(Takara, Japan)를 사용하였다. 실험 결과는 도 2에 도시하였다.
Human dermal fibroblasts were seeded in well plates at a density of 1 x 104 cells / cm 2 and incubated for 24 hours in an incubator (37 ° C, 5% CO2) for cell attachment and environmental adaptation . After the medium was removed, the medium without serum was treated and starvation was carried out for 8 hours. The composition of the Example was dissolved in the medium and treated for each sample. After the sample treatment, the human skin fibroblasts were cultured for 72 hours, and the supernatant of the culture medium was taken to quantify the synthesized collagen. For the determination of collagen, a Type I Procollagen C-Peptide EIA kit (Takara, Japan) was used. The experimental results are shown in Fig.
상기에서 살펴볼 수 있듯이, 본 발명의 식품 조성물은 펩타이드의 체내 합성을 촉진시킬 수 있는 구성 성분들을 함유함으로써, 콜라겐의 체내 합성을 촉진하고 피부 보습 효과를 증진시킬 뿐만 아니라, 제과류, 제빵류, 튀김류, 면류 또는 떡류 등의 다양한 곡류 식품에 적용시킬 수 있다.As can be seen from the above, the food composition of the present invention contains components capable of promoting the in vivo synthesis of peptides, thereby promoting the synthesis of collagen in the body and enhancing skin moisturizing effect, It can be applied to various kinds of cereal foods such as noodles or rice cakes.
본 발명은 상술한 특정의 실시예 및 설명에 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 발명이 속하는 기술 분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형 실시가 가능하며, 그와 같은 변형은 본 발명의 보호 범위 내에 있게 된다.
The present invention is not limited to the above-described specific embodiments and descriptions, and various modifications can be made to those skilled in the art without departing from the gist of the present invention claimed in the claims. And such modifications are within the scope of protection of the present invention.
Claims (7)
곡물 1000 중량부에 대하여, i) 콜라겐 효소분해 펩타이드 분말 5~7 중량%, 및 ⅱ) 히알루론산 1~3 중량%을 포함하는 식품 조성물.The present invention relates to a food composition which is used for cereal foods such as confectionery, bakery products, fried foods, noodles or rice cakes, promotes the synthesis of collagen in the body,
I) about 5-7% by weight of a collagen enzyme digesting peptide powder, and ii) 1-3% by weight of hyaluronic acid relative to 1000 parts by weight of cereal.
상기 콜라겐 효소분해 펩타이드가 체내에서 효율적으로 섬유아세포와 상호작용할 수 있도록, 상기 펩타이드의 평균 분자량이 1000Da 이하인 것을 특징으로 하는 식품 조성물.The method according to claim 1,
Wherein the peptide has an average molecular weight of 1000 Da or less so that the collagen enzyme degrading peptide can efficiently interact with fibroblasts in the body.
상기 콜라겐 효소분해 펩타이드 분말의 열적 안정성을 향상시키기 위하여, 상기 분말을 코팅액으로 코팅하여 마이크로캡슐화한 것을 특징으로 하는 식품 조성물.The method according to claim 1,
Characterized in that the powder is coated with a coating solution and microencapsulated to improve the thermal stability of the collagen enzyme degrading peptide powder.
상기 코팅액이 쉘락(Shellac), 제인-디피(Zein-DP) 또는 HPMC(hydroxypropyl methylcellulose) 중 어느 하나를 포함하며,
상기 코팅이 분무속도 0.5~1.0㎖/min, 공기 유입온도 60 내지 80℃ 및 분무압력 1.5 내지 2.5 ㎏/㎠의 유동층(Fluid Bed) 반응기에서 수행되는 것을 특징으로 하는 식품 조성물.The method of claim 3,
Wherein the coating solution comprises any one of Shellac, Zein-DP, and HPMC (hydroxypropyl methylcellulose)
Wherein the coating is carried out in a fluid bed reactor at a spray rate of 0.5 to 1.0 ml / min, an air inlet temperature of 60 to 80 ° C and a spray pressure of 1.5 to 2.5 kg / cm 2.
상기 펩타이드 분말과 코팅액의 비율이 1:1~3인 것을 특징으로 하는 식품 조성물.5. The method of claim 4,
Wherein the ratio of the peptide powder to the coating liquid is 1: 1-3.
콜라겐의 체내 합성을 촉진하기 위하여, 글루타민 펩타이드를 함유하는 단백질 가수분해물 1~6중량%를 추가로 포함하는 것을 특징으로 하는 식품 조성물.The method according to claim 1,
Wherein the composition further comprises 1 to 6% by weight of protein hydrolysates containing glutamine peptides in order to promote the synthesis of collagen in the body.
콜라겐의 체내 합성을 촉진하기 위하여, 비타민C 1~2 중량% 및 칼슘 1~2 중량%를 추가로 포함하는 것을 특징으로 하는 식품 조성물.
The method according to claim 1,
Characterized in that it further comprises 1 to 2% by weight of vitamin C and 1 to 2% by weight of calcium in order to promote the synthesis of collagen in the body.
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