KR20140073784A - Cosmetic composition with the extract of Solidago virgaurea L. var coreana Nakai, Aster tataricus, Saxifraga stolonifera - Google Patents

Abstract

The present invention relates to a cosmetic composition and, more specifically, to a cosmetic composition containing the extract of Solidago virgaurea, Aster tataricus, and Saxifraga stolonifera. The cosmetic composition is configured to contain mixture extract which is obtained by mixing and extracting the Solidago virgaurea, the Aster tataricus, and the Saxifraga stolonifera as an active ingredient. The present invention has great anti-inflammatory, effectiveness of anti-wrinkle, and antioxidant effectiveness.

Description

[0002] Cosmetic compositions containing extracts of Staphylococcus aureus L. var. Corea Nakai, Aster tataricus, Saxifraga stolonifera,

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition comprising an extract of Narakiri, anchovy, and orchid.

The skin is the largest organ of the body, always in direct contact with the external environment, and serves as a protective layer for protecting the living body from various stimuli or dry environment. It is an organ where new cells are generated and eliminated extensively as compared with other organs. In addition, it protects the body from physical abrasion, protects the inside of the body from moisture loss, protects it from ultraviolet rays generated from the sun, and plays a very important role in controlling body temperature.

These externalities, such as wrinkles, loss of elasticity, and keratinization, are caused by external stimuli due to various physical and external environmental factors. Skin aging is largely classified into natural aging (or endogenous aging) and external aging. Natural aging is difficult to artificially control because it is affected by genetic factors, whereas external aging is affected by environmental factors. Do.

Exemplary extrinsic aging factors include ultraviolet light and the most prominent external aging phenomenon is wrinkle formation (Daniell HW, Ann. Intern. Med., 1971, 75 (6), 873; Grove GL et al., J. Am. Acad Dermatol., 1989, 21 (3), 631; Griffiths CE et al., Arch. Dermatol., 1992, 128, 347). One of the photo-aging mechanisms caused by ultraviolet light is via free radical pathways (Harman D, J. Gerontol., 1956, 11 (3), 298).

Free radicals are known to cause disruption of connective tissue such as skin collagen, inhibition of cell membrane function, promotion of DNA mutation, modification of protein action, intercellular energy transfer, transformation of molecules related to metabolism (Lavker RM and Kligman AM, J. Invest. Dermatol., 1988, 90, 325). The fact that free radicals are involved in aging means that antioxidants that inactivate free radicals or a variety of chemical scavengers can be used to delay the aging process.

On the other hand, some free radicals and other active oxygen and peroxides are produced in normal cells in the metabolic process, but superoxide dismutase (SOD), catalase, And peroxidase as well as antioxidant substances such as vitamin C, vitamin E, glutathione, ubiquinone, and uric acid. However, oxidative stress is caused when an abnormality occurs in such a biological defense mechanism or when the production of active oxygen by various physical and chemical factors exceeds the capacity of a biological defense system. Therefore, suppression of active oxygen is an important factor in skin aging cosmetics.

Antioxidants such as tocopherol, polyphenols, Coenzyme Q 10, BHT and BHA have been extensively used for the purpose of neutralizing these reactive oxygen species. However, most of them are synthetic products. . Accordingly, a lot of studies for antioxidant activity, safety of human skin, and economic development of natural antioxidants of plant origin have been expected.

On the other hand, Narae Solidago Virgaurea (Solidago virgaurea L. var coreana Nakai) is a perennial plant with a height of 40-80㎝, and flowers bloom in July-October. Fruits are cylindrical with aqua, with little or no hair, and the length of the tangents is about 3.5 mm. It grows on mountains all over Japan, and is distributed in Japan, Manchuria, China, Taiwan, and the Philippines. The outpost is called Ichigo (枝花). It has the effect of picnic, blue fever, swelling, and detoxification. It treats cold, throat, jaundice, pertussis, pediatric seizures and boils. Ingredients include solidagosaponin, limonen, bornylacetate, and borneol.

Michaelmas daisy(Aster tataricus) Is a perennial plant with a height of 1 ~ 1.5m and a flower with a diameter of 2.5 ~ 3.3㎝. It runs from April to October at the tip of the branch and the tip of the main stem. The involucre is hemispherical, the bract is arranged in 3 lines, the tip is sharp-pointed, has short hairs, and the edges are dry. It is 16 ~ 17㎜ in length and 3 ~ 3.5㎜ in width and light blue. The fruit is an achene with a length of about 3mm, hairs, and a length of about 6mm. It is commonly found in mountain wetlands throughout Japan, and is distributed in Japan, Manchuria, China, Ussuri, Mongolia, and Dahriia. Roots and rootstocks are collected in autumn and dried. Roots and rootstocks are called resources, and have the efficacy of warming, doing, communicating, and inhabiting. It treats seawater, asthma and urinary incontinence caused by abundance. Ingredients include epifriedelanol, friedelin, shinone, astersaponin, quercetin, lachnophyllol, lachnophyllolacetate, and anethol.

Saxifraga stolonifera ) is a perennial plant with a height of about 40㎝, and the flower is white and blooms in May. The fruit is ovate with capsule. They are commonly found in rocky cottages and wetlands throughout Japan, and are distributed in Japan, Manchuria, and China. The outpost is called hoi-cho (耳 耳草), and it has the efficacy of giant wind, blue fever, and deciduous blood. Rubella, eczema, otitis media, solitary, hematocrit, hemorrhoids, hemorrhoids. Outposts contain arbutin, aesculin.

Korean Patent Publication No. 10-2012-0117115 (published on October 24, 2012) discloses a composition for promoting hair growth or preventing hair loss, which contains a humanized extract. Korean Patent Laid-Open No. 10-2002-0094349 (published on Dec. 18, 2002) discloses a whitening cosmetic comprising a mixture of peony root, upper limb and Hoiicho extract.

In the present invention, it is intended to develop a cosmetic composition using the above-described narakame, zooshi, and zoysac.

The present invention provides, as a first aspect, a cosmetic composition characterized by comprising, as an active ingredient, a mixed extract obtained by mixing Narakiri, Nagiso and Kozaku and extracting it.

In a second aspect, the present invention provides a cosmetic composition characterized in that it comprises, as an active ingredient, an extract of Namayama stalactite, an extract of Anthocyanin and an extract of a safflower.

The naramishi, the smell (sometimes referred to as "good") and the smell (referred to as "hoicho") used in the present invention can be easily purchased through conventional markets such as Kyungdong Market or Internet market.

It is preferable to wash the dried narakiri, eggplant (jawan) and oyster (hoicho) used in the present invention with purified water, preferably by washing with purified water.

In the present invention, the extraction solvent is selected from the group consisting of water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol and mixed solvents thereof (for example, Two solvent mixtures, or two or more solvent mixtures) may be used, and it is preferable that the solvent is extracted with a solvent selected from water, anhydrous alcohol having 1 to 4 carbon atoms, or hydrated alcohol. Examples of the alcohol having 1 to 4 carbon atoms include methanol, ethanol, n-propanol, iso-propanol, n-butanol and tert-butanol. Hydroxy ethanol is most preferred in these extraction solvents, and the amount of water contained is preferably within 30 to 90 (wt / wt)%, more preferably 70 (wt / wt)%.

Mixed extracts of Nalamis, Chrysanthemum, and Zoysia (Hoiicho) of the present invention, or each individual extract, can be prepared by known extraction methods known to those of ordinary skill in the art. For example, in the present invention, dried (or all mixed) dried nira bean sprouts, red bean sprouts and lozenges (hoi choco) are separately fired, and water of 1 to 50 times their dry weight, 4 of anhydrous alcohol or hydrated alcohol is added and the mixture is immersed at 4 to 30 DEG C for 3 to 20 days to extract an active ingredient and then the extraction solvent can be obtained by concentrating the extractant with a reduced pressure concentrator.

In addition, in the present invention, the dried (dried) narakame, the dried bean sprouts and the dried bean sprouts are pulverized and mixed with water of 1 to 30 times the dry weight, At least one solvent selected from the group consisting of anhydrous alcohol and hydrated alcohol is used. Then, in order to prevent the solvent from evaporating, it is heated and extracted with an extractor equipped with a cooling condenser at 30 to 100 DEG C for 1 to 48 hours, Lt; 0 > C for 1 to 10 days to extract the active ingredient and concentrate the extraction solvent with a vacuum concentrator. It is also possible to extract by using the ultrasonic extraction method known in the above-mentioned extraction solvent. However, it is preferable that the extract of Nakayama mashii, red mackerel and rosemary extract of the present invention or each individual extract is extracted with water, anhydrous or hydrous alcohol having 1 to 4 carbon atoms, 100 deg. C is preferable.

In the present invention, in the present invention, the mixed extract of Angelica gigas Nakai, Angelica gigas Nakai and Kozaku (Hoi chia) or each individual extract is obtained by concentrating the extract solution obtained by extracting with the extraction solvent, the freeze-dried powder or concentrate of the concentrate by spray drying, Lt; / RTI >

On the other hand, in the cosmetic composition of the present invention, the mixed extract of Narakami, Nagase and Zoysaccharide is added to the cosmetic in an amount of 0.001% to 10% by weight in powder form or liquid form relative to the total weight of the cosmetic composition And may be added to the cosmetic in an amount of 0.01 to 5% by weight, preferably in powder form or in liquid form, based on the total weight of the cosmetic composition.

On the other hand, the present invention can separately prepare extracts for each of Narakiri, Nagase and Zoysia, and then add them to the cosmetic composition to mix these components. The addition amount thereof is such that the sum of the three components is from 0.001% to 10% by weight, preferably from 0.01% by weight to 5% by weight, based on the total weight of the cosmetic composition, in powder or liquid form, %. ≪ / RTI > At this time, the blending ratio of the three components can be variously adjusted.

On the other hand, the cosmetic composition of the present invention is not particularly limited in its formulation, and examples thereof include cosmetic lotion, nutrition lotion, nutritional cream, massage cream, essence, pack, emulsion type foundation, solid type foundation, It can have a formulation. It may also be a form of bath cleanser, shampoo, soap, and the like. In addition, in the cosmetic composition of each formulation, components other than the above-mentioned extracts of Narakiri, Chrysanthemum, and Poisonous (or Hoiicho) extracts (or mixed extracts) can be arbitrarily selected and formulated according to the formulation or use purpose of other cosmetics have. For example, it may include a coloring agent (coloring matter), a flavoring agent (fragrance), a suspending agent, an emulsifying agent, a solubilizing agent, a stabilizer, an isotonic agent, a pH adjusting agent, a viscosity adjusting agent,

On the other hand, the cosmetic composition of the present invention is preferably used for anti-inflammation, anti-wrinkle treatment, anti-aging, anti-inflammation, anti-wrinkle or antioxidant, May contain one or more other active ingredients exhibiting an effect.

The cosmetic composition of the present invention, which contains a mixed extract of Narakamishi, Anisuga (Zykwan) and Zoysac (Hoicho), was found to be excellent in anti-inflammatory, wrinkle-reducing and antioxidative effects.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following embodiments, and includes modifications of equivalent technical ideas.

[ Example  One: Naraka seaweed , Wisdom ) And rosewood Hoi Cho ) Preparation of mixed extract powder [

After washing with the purified water, the dried seaweed, 100 grams of dried narakame, 100 grams of dried seaweed, 100 g of dried seaweed (Hoicho) and 6 kg of distilled water were added to the dried seaweed, And extracted from the extractor equipped with a condenser at 80 ° C to 100 ° C for 2 hours. This process was repeated three times. Thereafter, the mixture was filtered through a 300-mesh filter paper, left at room temperature for 1 week, and the precipitate was filtered twice with a filter paper of Edbentek No. 5 and a Wattman GFC 150 mm filter. Then, the mixture was concentrated at a temperature of 40 to 50 ° C using a vacuum concentrator, and 20 g of dried extract powder was obtained by using a spray dryer (model name: B-290, manufactured by BUCHI) under the following conditions.

-Inlet temperature: 180 DEG C

-Aspirator efficiency: 100% (35 cm3 / hr)

-Pump efficiency: 25% (7.5 ml / min)

-Nozzle Cleaner 4

-Rotameter: 30 mm (357 Liter / hr)

[ Example  2 to 11: Naraka seaweed , Wisdom ) And rosewood Hoi Cho ), Preparation of mixed extract powder according to extraction solvent and preparation of mixed extract powder according to mixing ratio]

Mixed extract powder was prepared in the same manner as in Example 1 except that the extraction solvent and the addition ratio were varied for the mixture of Narakiri, Zoysia (Zykwan) and Zoysac (Hoicho). The extraction solvents used were as shown in Table 1 below. At this time, in Examples 2 to 5 shown in Table 1, the Narakiri, Zoyu (zygwan), and Zoysac (hoicho) were mixed at a weight ratio of 1: 1: 1, respectively.

Meanwhile, after confirming the effect of the extraction solvent, the extract powder was prepared at various mixing ratios as shown in Table 2, using the 70% ethanol of Example 4, which showed excellent effects.

division Extraction solvent Example 2 30% ethanol Example 3 50% ethanol Example 4 70% ethanol Example 5 100% ethanol

division Ratio of medicinal ingredients (Narayashi: Example 6 2: 1: 1 Example 7 1: 2: 1 Example 8 1: 1: 2 Example 9 2: 2: 1 Example 10 1: 2: 2 Example 11 2: 1: 2

[ Experimental Example  One: Xanthine  Oxidase ( Xanthine Oxidase ) Inhibitory effect confirmation]

The Nitro-Blue Tetrazolium (NBT) reduction method was used to measure the scavenging activity of superoxide radicals produced by xanthine oxidase.

After mixing 1 ml of 0.1 M potassium phosphate buffer (pH 7.5) containing 0.4 mM xanthine and 0.24 mM NBT and 0.6 ml of 0.1 M potassium phosphate buffer (pH 7.5) and 0.1 ml of the sample, xanthine oxidase (0.05 U / ml ) And the mixture is reacted at 37 ° C for 20 minutes. After the reaction was terminated by adding 1 ml of 1 M HCl, the absorbance at 520 nm was measured.

The NBT reduction inhibition was calculated by the following equation (1), and the results are shown in Table 3 below.

[Equation 1]

B (Blank): Absorbance of reaction solution containing 0.1M potassium phosphate buffer (pH 7.5) containing 0.4mM xanthine and 1M HCl alone

C (Control): absorbance of the reaction solution to which 0.24 mM NBT was added instead of the sample

S (Sample): absorbance of the reaction solution to which the sample is added

division NBT reduction efficiency (%) 0.1% 0.2% 0.5% 1.0% 2.0% 5.0% Example 1 10 11 20 33 43 60 Example 2 13 13 26 37 47 65 Example 3 12 15 30 41 50 78 Example 4 25 43 50 57 64 84 Example 5 11 12 21 32 40 55 Example 6 10 11 20 31 59 80 Example 7 15 19 32 43 52 76 Example 8 17 20 29 40 57 76 Example 9 8 10 23 33 45 78 Example 10 10 11 20 32 41 75 Example 11 9 14 18 30 43 79

[ Experimental Example  2: Superoxide Dismutase ( superoxide Dismutase , SOD ) Identify the effect of similar activity]

SOD-like activity was determined by measuring the amount of pyrogallol produced that catalyzed the conversion of hydrogen peroxide according to the method of Marklund S. et al. Eur. J. Biolchem., 47, p468, 1975) Similar activity. 3 ml of Tris-HCl buffer (50 mM Tris [hydroxymethyl] amino methane, 10 mM EDTA, pH 8.5) and 0.2 ml of 7.2 mM pyrogallol were added to 0.2 ml of the sample and reacted at 25 ° C for 10 minutes. 1 ml of 1N HCl was added Lt; / RTI > The amount of oxidized pyrogallol in the reaction solution was measured at 420 nm.

SOD-like activity was calculated by the following equation (2), and the results are shown in Table 4 below.

&Quot; (2) "

division SOD-like activity (%) 0.1% 0.2% 0.5% 1.0% 2.0% 5.0% Example 1 3 5 12 20 31 39 Example 2 6 15 24 29 46 55 Example 3 7 19 31 44 63 78 Example 4 11 28 42 60 75 91 Example 5 3 4 10 17 25 33 Example 6 11 18 33 48 63 80 Example 7 7 15 31 44 60 75 Example 8 8 14 32 40 59 73 Example 9 5 11 31 41 59 71 Example 10 6 12 30 42 61 76 Example 11 5 10 29 43 62 77

[ Experimental Example  3: Nitrite ( NO ) Confirmation of scavenging activity effect]

Nitrite (NaNO ₂ ) scavenging activity was measured according to the method of Kato et al., (Agric. Biol. Chem. 51, p1333, 1987). A sample was added to 2 ml of 1 mM sodium nitrite (NaNO ₂ ) solution, and 0.1 N HCl (pH 1.2) and 0.2 M citrate buffer were used. The pH of the reaction solution was adjusted to 1.2, 3.0 and 6.0, respectively, and the volume of the reaction solution was adjusted to 10 ml. The reaction solution was reacted at 37 ° C for 1 hour. One ml each of the reaction solution was added thereto and 5 ml of 2% acetic acid was added . Then, 0.4 ml of a grease reagent (A: B = 1: 1, A: 1% sulfanilic acid in 30% acetic acid, B: 1% naphthylamine in 30% acetic acid) was added and reacted at room temperature for 15 minutes. Absorbance was measured at 520 nm after the reaction.

The nitrite scavenging activity was calculated by the following equation (3), and the results are shown in Table 5 below.

&Quot; (3) "

division Nitrite scavenging activity (%) 0.1% 0.2% 0.5% 1.0% 2.0% 5.0% Example 1 4 7 10 15 19 27 Example 2 6 9 14 22 37 50 Example 3 8 20 28 35 41 57 Example 4 11 24 33 41 59 74 Example 5 3 8 9 13 20 26 Example 6 5 9 14 26 36 51 Example 7 6 9 15 22 31 50 Example 8 4 7 13 20 34 52 Example 9 6 5 14 21 33 49 Example 10 7 8 12 21 30 45 Example 11 6 6 13 20 31 47

[ Experimental Example  2: NO  Confirming production inhibition effect]

In order to confirm the anti - inflammatory effect, the effect of inhibiting nitric oxide (NO) production, which is known to play an important role in inflammation induction, was examined. Although normal NO formation plays an important role in killing bacteria or eliminating tumors, NO produced by iNOS in the inflammatory state not only promotes inflammatory responses such as vascular permeability and edema, but also stimulates inflammation mediator biosynthesis, It is known to intensify.

Raw 264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% cotton, and inoculated on a 24-well plate. LPS (Lipopolysaccharide, 1 μg / ml) And cultured for 24 hours. The amount of NO produced was calculated by the following formula using the NO detection kit (iNtRON). The control group was an experimental group treated with LPS alone.

The NO generation inhibition rate was calculated by the following equation (4), and the results are shown in Table 6 below.

&Quot; (4) "

division NO production inhibition rate (%) 0.5% 1.0% 2.0% 5.0% Example 1 0 13 24 41 Example 2 7 17 27 46 Example 3 11 21 30 57 Example 4 28 37 44 65 Example 5 5 12 20 36 Example 6 7 18 37 55 Example 7 8 14 31 50 Example 8 6 15 30 53 Example 9 7 16 33 54 Example 10 5 18 32 52 Example 11 6 17 31 50

[ Experimental Example  3: Confirmation of inhibition of prostaglandin production]

The inhibitory effect of prostaglandin E ₂ (prostaglandin E ₂ , PGE ₂ ), which is known to play an important role in inflammation induction, was examined. Cyclooxygenase (COX) is an enzyme that converts arachidonic acid into prostaglandin, classified as COX-1 and COX-2. COX-1 acts on normal biological functions such as platelet formation, gastric wall protection and maintenance of renal function in the body, and COX-2 forms PGE ₂ , an inflammation mediator. PGE2 is known to be deeply involved in the development of cancer, including inflammation, immune response, and angiogenesis.

Raw 264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% cotton, and inoculated on a 24-well plate. LPS (Lipopolysaccharide, 1 μg / ml) And cultured for 24 hours. The amount of PGE ₂ produced was calculated using the PGE ₂ ELISA kit (R & D systems, Inc, USA) using the following equation. The control group was an experimental group treated with LPS alone.

The inhibition rate of PGE ₂ production was calculated by the following equation (5), and the results are shown in Table 7 below.

&Quot; (5) "

division Inhibition rate of PGE ₂ production (%) 0.5% 1.0% 2.0% 5.0% Example 1 10 18 22 46 Example 2 9 13 22 37 Example 3 14 18 26 56 Example 4 31 39 47 61 Example 5 14 15 33 41 Example 6 7 15 29 47 Example 7 10 18 30 48 Example 8 8 17 24 45 Example 9 9 14 25 40 Example 10 6 18 27 41 Example 11 7 16 26 44

[ Experimental Example  4: TNF -α generation inhibitory effect]

In order to confirm the anti-inflammatory activity, the inhibitory effect of TNF-α, one of the early inflammatory molecules, was confirmed. The formation of pro-inflammatory cytokines, such as TNF-α, is mediated through the process of converting arachidonic acid into prostaglandin due to the activity of phospholipase A2 (A2) .

Raw 264.7 cells were adjusted to 5 × 10 ⁵ cells / well using DMEM medium supplemented with 10% FBS and inoculated into a 24-well plate. LPS (Lipopolysaccharide, 1 μg / ml) And cultured for 24 hours. The amount of TNF-α produced was measured using a TNF-α ELISA kit (eBioscience, Inc, USA) using the following formula. The control group was an experimental group treated with LPS alone.

The inhibition rate of TNF-? Production was calculated by the following equation (6), and the results are shown in Table 8 below.

&Quot; (6) "

division TNF-α production inhibition rate (%) 0.5% 1.0% 2.0% 5.0% Example 1 8 14 25 38 Example 2 10 18 29 41 Example 3 17 21 30 45 Example 4 31 39 47 59 Example 5 16 12 20 36 Example 6 11 20 38 47 Example 7 9 19 39 51 Example 8 10 15 36 49 Example 9 11 18 35 50 Example 10 10 17 33 48 Example 11 9 16 34 45

[ Experimental Example  5: Identification of inhibition of inflammatory reaction induced by ultraviolet light]

Human keratinocytes were inoculated into DMEM medium containing penicillin (100 IU / ml), streptomycin (100 g / ml) and 10% FBS (fetal bovine serum) ≪ / RTI > The keratinocytes obtained were divided into 3 × 10 ⁵ cells per well in a 6-well plate and cultured for 24 hours. After incubation, the cells were irradiated with 25 mJ / cm 2 of ultraviolet light, added with the sample, and further cultured for 24 hours. The amount of TNF-α and IL-1α produced was calculated using an ELISA kit (eBioscience, Inc, USA) using the following formula. The control group was an experimental group treated with ultraviolet light alone.

The inhibition rates of TNF-? And IL-1? Production were calculated by the following equations (7) and (8), and the results are shown in Tables 9 and 10 below.

&Quot; (7) "

&Quot; (8) "

division TNF-α production inhibition rate (%) 0.5% 1.0% 2.0% 5.0% Example 1 4 10 20 33 Example 2 8 14 25 41 Example 3 9 14 31 45 Example 4 21 28 40 48 Example 5 9 14 22 35 Example 6 10 15 29 39 Example 7 11 17 28 40 Example 8 9 16 30 42 Example 9 7 15 31 40 Example 10 10 13 28 41 Example 11 8 14 29 37

division Inhibition rate of IL-1-α production (%) 0.5% 1.0% 2.0% 5.0% Example 1 0 2 11 30 Example 2 2 8 15 31 Example 3 One 10 21 44 Example 4 5 18 32 46 Example 5 0 7 14 30 Example 6 7 12 24 35 Example 7 4 9 29 40 Example 8 7 11 26 39 Example 9 5 8 25 37 Example 10 6 10 24 38 Example 11 3 7 19 36

[ Formulation Example  One: Naraka seaweed , Wisdom ) And rosewood Hoi Cho ) Preparation of Lotion Containing Mixed Extracts]

1 kg of cosmetic lotion (skin lotion) containing the extract of Nakayama seaweed, Angelica keiskei (jawan), and oyster (Hoiicho) mixed extract powder (Example 4) was prepared in the following Table 11 using the following production method.

number Raw material Content (% by weight) One (Example 4) mixed extract powder of Nakayama mushroom extract (Jawan) and Zoysac (Hoicho) 1.0 2  glycerin 3.0 3  Butylene glycol 2.0 4  Propylene glycol 2.0 5  Polyoxyethylene hardened castor oil 1.0 6  ethanol 10.0 7  Citric acid 0.02 8 Sodium citrate 0.06 9  antiseptic a very small amount 10  Spices a very small amount 11  Purified water Balance

In Table 11, the substances No. 2, 3 and 4 were sequentially added to the 11th substance in the reaction vessel, and they were dissolved by stirring. Then, the substances No. 5 and 6 were dissolved by heating at about 50 ° C. Subsequently, the substance 10 was added to the substance 11 after dissolution. Finally, the materials 1, 7, 8, and 9 were added, stirred thoroughly, and aged at 25 ° C for 3 days to prepare the title lotion.

[ Formulation Example:  2] Naraka seaweed , Wisdom ) And rosewood Hoi Cho Manufacture of nutrition lotions containing mixed extracts

1 kg of a hydrosomonal nutrient lotion containing the extracts of Narakamichi, Nagase (Jawan), and Kozo (Hoi-cho) mixed extract powder (Example 4) was prepared in the following Table 12 using the following production method.

number Raw material Content (% by weight) One (Example 4) mixed extract powder, 1.0 2  Wax 1.0 3  Polysorbate 60 1.5 4  Sorbitan sesquioleate 0.5 5  Liquid paraffin 10.0 6  Sorbitan stearate 1.0 7  Pro-type glycerin monostearate 0.5 8  Stearic acid 1.5 9 Glyceryl stearate / phage-100 stearate 1.0 10 Propylene glycol 3.0 11 Carboxyvinyl polymer 0.1 12 Triethanolamine 0.2 13 antiseptic a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16  Purified water Balance

In Table 12, materials 10, 11, 13, and 16 were heated to 80 to 85 ° C while mixing and stirring. Then, the materials were charged into the manufacturing part and then emulsified with 2, 3, 4, 5, 6, 7, 8, 9, The substance No. 12 was dissolved by heating at a temperature of 80 to 85 ° C and emulsified. After the emulsification is completed, the mixture is cooled to 50 ° C with stirring using an agitator, and then the substance 15 is added. After cooling to 45 ° C, the substance 14 is added and the substance 1 is added at 35 ° C. For 3 days to prepare the nutritional lotion of the title.

[ Formulation Example  3: Naraka seaweed , Wisdom ) And rosewood Hoi Cho Nutrition with mixed extracts Serum  Produce

1 kg of a hydrosomal nutrition serum containing the extracts of Narakamichi, Nagase (Jawwan) and Kozo (Hoiicho) mixed extract powder (Example 4) was prepared in the following Table 13 using the following preparation method.

number Raw material Content (% by weight) One Nara-michi, Zenishi (Zawan) and Kozu (Hoi-cho)
The mixed extract powder (Example 4) 1.0 2 Xanthan gum 0.02 3 Butylene glycol 10.0 4 Phospholipid 10.0 5 Hydroxyethyl cellulose 0.1 6 Sodium hyaluronate 5.0 7 antiseptic a very small amount 8 Spices a very small amount 9 Purified water Balance

In Table 13, substances 2, 3, 5 and 9 were mixed and stirred while being heated to 40 to 45 ° C to be introduced into the production section. Then, an emulsifier was allowed to react and the substances 4, 6, 7 and 8 were added to the production section . After mixing, the mixture was cooled to 35 ° C with stirring using an agitator, the material 1 was added thereto, cooled to 25 ° C, and aged at 25 ° C for 3 days to prepare the titled nutritional serum.

[ Formulation Example  4: Naraka seaweed , Wisdom ) And rosewood Hoi Cho ) Preparation of Essence Containing Mixed Extract]

1 kg of hydrosomal essence containing Narakamishi, Chrysanthemum (Zykwan) and Zygomyo (Hoiicho) mixed extract powder (Example 4) was prepared using the following preparation method in the contents shown below in Table 14 below.

number        Raw material Content (% by weight) One (Example 4) mixed extract powder, 1.0 2 Cetearyl alcohol 1.0 3  Polyglyceryl 2-oleate 0.8 4  Ceramide 0.1 5 Steareth-20 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 Concentrated glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Triethanolamine 0.2 12  Xanthan gum 0.2 13  antiseptic a very small amount 14  Spices a very small amount 15  Purified water Balance

In Table 14, materials 2, 3, 4, 5 and 6 were homogenized at a constant temperature to prepare nonionic amphipathic lipids. This nonionic amphipathic lipid was mixed with substances 1, 7, 8 and 15, homogenized at a constant temperature and passed through a microfluidizer. Subsequently, the substance No. 9 was heated to 50 to 60 ° C and gradually added to homogenize And again passed through the microfluidizer. Subsequently, substances 10, 11, 12, 13 and 14 were added, dispersed, stabilized and aged to prepare the title essence.

[ Experimental Example  6: Confirmation of formulation stability]

(1) to (4), which are kept constant at room temperature (25 ° C), cold (4 ° C) and constant temperature (45 ° C) for Formulation Examples 1 to 4, And incubated in an opaque glass container in an incubator for 12 weeks to store and observe (discoloration, detachment and phase separation) and to confirm stability.

Temperature
Condition Stability verification (discoloration, removal and separation) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Indoor (25 ℃) 0 0 0 0 Refrigerated (4 ℃) 0 0 0 0 Constant temperature (45 ° C) 0 0 0 0

<Formulation stability level>

0: No change, 1: Minute change, 2: Change, 3: Extreme change

As shown in Table 15, all Formulation Examples 1 to 4 formulations were stable without deterioration of coloration, separation and separation under the temperature conditions of 25 占 폚, 4 占 폚 and 45 占 폚.

Claims (6)

Wherein the cosmetic composition comprises a mixed extract obtained by mixing and then extracting a mixture of Narakiri, Nagara and Zoysaccharum, as an active ingredient.
Wherein the extract comprises an extract of Narakiri marigold, an extract of Anthocyanin and an extract of a marigold extract as an active ingredient.
3. The method according to claim 1 or 2,
In the cosmetic composition,
Wherein the composition is for relieving inflammation.
The method according to claim 1 or 2, wherein
In the cosmetic composition,
Wherein the cosmetic composition is for improving wrinkles.
3. The method according to claim 1 or 2,
In the cosmetic composition,
Wherein the cosmetic composition is for preventing aging.
3. The method according to claim 1 or 2,
The above-
A cosmetic composition characterized by using a functional alcohol as an extraction solvent.