KR20110130115A - Cosmetic composition containing the extract of angelica koreanum with the anti-inflammatory and antioxidative effects - Google Patents

Abstract

PURPOSE: An Angelica Koreanum extract with anti-inflammation and antioxidation and a cosmetic composition containing the same are provided to suppress the generation of NO without skin irritation. CONSTITUTION: A cosmetic composition for anti-aging contains 5-30 wt% of Angelica Koreanum extract. The extract is isolate by adding water, hydrophilic organic solvent, or mixture solvent thereof to Angelica Koreanum. The extract is prepared using purified water, anhydrous or hydrous methanol of 1-4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol, or butylene glycol. The cosmetic composition is manufactured in the form of a skin softener, nutrition lotion, nutritional cream, massage cream, essence, pack, emulsion, oil gel, and mask pack.

Description

Active extract with anti-inflammatory and antioxidant effects and cosmetic composition containing same {Cosmetic composition containing the extract of Angelica Koreanum with the Anti-inflammatory and Antioxidative effects}

The present invention relates to an active extract having an anti-inflammatory and antioxidant effect and a cosmetic composition containing the same, and more particularly, there is no irritation and safety on the skin, and there is no problem of inducing skin diseases, and the production of nitric oxide is suppressed. In addition to showing an anti-inflammatory effect, it relates to a skin anti-aging cosmetic composition exhibiting an antioxidant effect through the ability to eliminate reactive oxygen species (Reactive Oxygen Species).

In general, cosmetics make skin healthy and beautiful, and play a role in delaying exogenous aging caused by endogenous aging and external factors caused by aging.

The skin is the most external tissue of the body, and it is an indispensable organ for maintaining the biochemical functions necessary for the metabolism of the human body while protecting the body from water and chemical factors of the external environment. Specifically, the skin secretes sweat, sebum, and waste products as well as endocrine function, immune function, absorption and secretion function, and protection function. D Plays an important role in performing a wide variety of functions, including synthesis and nutrient storage. These skins are easily damaged by external environments such as physicochemical injuries, microorganisms such as bacteria and fungi, parasite infections, UV exposure, and air pollution, which are closely related to aging of the skin.

When the skin is damaged, inflammation occurs, a phenomenon in which blood components escape through the walls of blood vessels into the tissue as a local protective response to damage to biological tissues. Through the inflammatory response, the human body secretes various cellular products such as proteolytic enzymes and cytokines, thereby treating and defending against inflammation. Usually, inhibitory proteins called transcription factors NF-kB and ikb, which promote the expression of cytokine receptors and genes involved in cell adhesion substance production, exist in the form of a complex. However, when stimulation is applied to cells using ultraviolet light or lipopolysaccharide (LPS, lipopolysaccharide), an enzyme called IKK acts to phosphorylate the complex of NF-kB and ikb, resulting in a drop in ikb. NF-kB, which migrates into the nucleus of the cytoplasm, binds to precursors of inflammation-related genes and induces transcription of the genes. In this case, induced nitric oxide synthase (iNOS) and cyclo, which are inflammation-associated biosynthetic enzymes, are produced. Nitric oxide and prostaglandin are synthesized by oxidase-2 (COX-2) and released into the cell. Excessive inflammatory reactions damage the surrounding cells and connective tissues by proteolytic enzymes, and if this process continues or chronically leads to serious tissue damage and causes rapid skin aging.

In general, skin aging is associated with somatic mutations, excessive production of free radicals, autoimmune reactions, accumulation of toxic substances, errors in gene duplication, and modification of dermal constituent proteins (M. Rieger, Cosmetics & toiletries, 110, 94, 1994). Various theories have been raised, and among them, active oxygen species derived from oxygen or nitrogen have been recognized as a theory that the causes of various diseases such as aging and adult diseases are caused by reactive oxygen species. Research to develop a natural antioxidant that can control the activity is actively underway. Active oxygen species, commonly known as hazardous oxygen, are the most stable forms of triplet oxygen ( ³ O ₂ ) with reduced peroxide anion radicals (O _2- ^· ), hydrogen peroxide (H ₂ O ₂ ), hydroxy radicals (OH) , Lipid peroxides (ROOH) or free radicals (ROO ·, RO ·) generated here, such as free radicals in the body that occur in normal conditions, but the antioxidant enzyme superoxide dismutase (SOD) ), Catalase, glutathione peroxidase (GPx), etc. are present to inhibit the generation of excessive active oxygen species. However, when these reactive oxygen species are abnormally excessively generated due to stress and external environmental factors and exist in the body without being erased, oxidative stress caused by free radicals is applied to the living body, resulting in DNA, RNA, protein, It is estimated to cause cell membrane and cell structure damage, cancer, tissue damage, aging and the like.

In order to suppress the acceleration of various diseases and aging rapidly increasing in modern times, there is an active research on antioxidants that can control or eliminate such reactive oxygen species. Typically, studies on the efficacy of natural antioxidants, tocopherol, ascorbic acid, carotenoids, flavonoids, glutathione, and the like and their stabilization techniques, and synthetic antioxidants butylated hydroxy toluene (BHT), butylated hydroxy anisol Various antioxidants such as (BHA) are known, and research on many other antioxidants is ongoing. (EA Lissi et al. Free Radic. Biol. Med, 18 (2), 153, 1995) (A. Ghiselli et al. Free Radic. Biol. Med, 18 (1), 29, 1995) Tocopherols among these antioxidants Has a relatively low antioxidant effect, and synthetic antioxidants such as BHA and BHT, which have excellent effects, have been used in medicine and food fields, but there are indications of mutagenicity and toxicity, and derivatives and formulations with stability in ascorbic acid. As development proceeds, there is an urgent need for the development of more stable and effective natural antioxidant materials.

It is well known that plants contain various active ingredients, and many plant extracts are known to be effective in relieving pain, detoxifying, antipyretic, antiseptic, astringent, anti-inflammatory, and antioxidant. According to the trend, the active ingredients in cosmetics are also used in various forms in cosmetics based on their usefulness as well as chemicals and natural substances derived from plants.

The present invention relates to an active extract having an anti-inflammatory and antioxidant effect and a cosmetic composition containing the same, and more particularly, there is no irritation and safety on the skin, and there is no problem of inducing skin diseases, and the production of nitric oxide is suppressed. In addition to showing an anti-inflammatory effect, an object of the present invention is to provide a skin anti-aging cosmetic composition exhibiting an antioxidant effect through the ability to eliminate reactive oxygen species (Reactive Oxygen Species).

In order to achieve the above object, the present invention provides an anti-aging cosmetic composition according to the anti-inflammatory effect and antioxidant effect, comprising the active extract as an active ingredient.

As a result of applying the active extract to the cosmetic composition in the present invention, while excellent anti-inflammatory and antioxidant efficacy, while ensuring the safety does not cause irritation to the skin to complete the present invention.

Angelica Koreanum belongs to the Minari family, and grows in mountainous and mountainous wetlands and valleys in the central and northern regions of Korea (Gyeongbuk, Gangwon, Gyeonggi, Pyeongbuk, Hamnam, and Hambuk), and is distributed geographically in Manchuria and Ussuri. Especially, it grows in the shaded humid place under the broad leaf tree in the deep valley of north central and Gangwon-do. Perennial herb, about 2m high, stems upright, hairless, branched from above, similar to parsley, but different from upper part. Flowers bloom in August-September, small white flowers gather in an umbrella shape. Boksan-style inflorescences are developed at the end of branches and main stems, and are divided into 10-30 scattered flowers. In this run, 1-2 guns are lanceolate, and there are about 6 small guns and linear. Petals are 5 and there are 5 stamens. Fruits mature in October, branch is oval, flat, with broad wings. Among the ingredients contained in the actives, Coumarin contains 0.26 (0.98)% in the root, 0.11% in the outpost and 0.13-0.44% in the fruit. Angelicoreanol, bisorarangeron, isoimferatorin, oxyfejudtanin, imperatorin, ostol, ferulic acid, osrenol (recording point 126 ° C, 7-hydroxy-8-pentenylcoumarin), verber Gapten, Angelicin, Umbelliferon and Angelicol were isolated. The essential oil content is 0.39% in the root, 1.13% in the fruit, 0.67% in the ear of flowers and 0.06% in the leaves.

The active extract used in the cosmetic composition of the present invention can be obtained by adding 95% ethanol, 70% ethanol and hot water to the active pulverized product, wherein the activity is preferably included in the range of 5 to 30% by weight.

In addition, the extraction solvent used to obtain the primary extract is from a solvent group including purified water, anhydrous or hydrous methanol having 1 to 4 carbon atoms, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol One pure solvent or two or more selected solvents may be used, but the present invention is not limited thereto. The amount of the extraction solvent added is preferably 1 to 20 times the total volume of the mixed herbal medicines. This extraction step may be performed by applying a conventional extraction method of the herbal medicine, which will be apparent to those skilled in the art having ordinary knowledge in the art.

The goliathic fermented extract obtained as described above may be included in the range of 0.1 to 90% by weight, preferably 1 to 80% by weight in the anti-aging cosmetic composition of the present invention. If the content is less than 1% by weight it is difficult to expect a substantial skin effect, higher than 80% by weight has problems in cosmetic formulation and manufacture.

According to the present invention, the formulation of the anti-aging and anti-aging cosmetic composition containing an active extract as an active ingredient is not particularly limited and may be appropriately selected as desired. For example, the cosmetic composition of the present invention may be prepared in formulations such as flexible cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, pack, emulsion, oil gel, mask pack.

In addition, the skin anti-aging cosmetic composition of the present invention may be formulated into general skin cosmetics in addition to the formulations described above, and may require oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, or fragrances. According to the formulation, it can be used in combination.

Skin protection composition of the present invention has the effects of anti-inflammatory, antioxidant, stimulating relieving power at the same time can be widely used for the purpose of skin protection, such as recovery and improvement of damaged skin in medicine, cosmetics, food and the like. Specifically, in medicine, it can be used for anti-inflammatory agents and anti-aging products related to antioxidant. However, the skin protection composition of the present invention is not limited to this purpose.

The present invention solves the problems of the prior art as described above is hypoallergenic and has no problem in inducing skin diseases, has excellent anti-nitrification and hydroxyl radical scavenging ability and exhibits anti-inflammatory and antioxidant effects in the form of a skin anti-aging cosmetic composition The stability of the cosmetics can be added and used in all forms.

1 is a graph showing the hydroxyl radical (Hydroxyl radical) scavenging ability of the active extract.
2 is a cell viability graph of the active extract.
Figure 3 is a graph of nitric oxide (Nitric Oxide) production inhibitory activity of the active extract.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited only to the following examples, and includes modifications of equivalent technical ideas.

Example  1. Active extract manufacture

The dried actives were chopped and crushed again to obtain a powder. 95% ethanol was added to the obtained active powder by 5 to 30 times by weight, and extracted by stirring for 3 hours at room temperature, and the extract was recovered using a filter paper. The recovered extract was obtained by using a rotary vacuum concentrator to obtain the active ingredient in the extract, which was used for the test after undergoing a freeze drying process.

Test Example  One. Hydroxyl Radical ( Hydroxyl radical ) Scatters

The hydroxyl radical scavenging ability of the active extract prepared in Example 1 was measured using an electron paramagnetic resonance method (Electron Paramagnetic Resonance, Kangwon National University). The addition of hydrogen peroxide and divalent iron salts generates OH, which in turn reacts with DMPO to produce DMPO-OH radicals. By measuring the EPR signal of DMPO-OH, the antioxidant activity of the extract against hydroxyl radicals can be investigated. In the experiment with the extract, if there is antioxidant activity, the EPR signal is weakened by scavenging hydroxyl radicals. This can be used to quantitatively determine the hydroxyl radical scavenging effect of certain substances. In the present invention, the hydroxyl radical was generated by the Fenton reaction and then quantified by EPR method using DMPO spin trap. Extracts were added and their hydroxyl radical scavenging ability was measured by the reduction of the EPR signal, wherein the antioxidant activity was expressed relative to the EPR signal when the extract was added, based on the EPR signal of the control group without the extract. As a positive control, Quercetin, an antioxidant activity substance, was used, and the hydroxyl radical scavenging ability (%) according to the concentration of active extracts compared to Quercetin is shown in Table 1.

Sample concentration (μg / ml) Hydroxyl radical scavenging ability (%)
(Quercetin 100μg / ml) 10 14.7 ± 0.8 20 30.4 ± 2.3 50 53.2 ± 5.2 100 78.8 ± 6.8

As shown in Table 1, the active 95% ethanol extract prepared in Example 1 can be confirmed that the hydroxy radical scavenging ability is excellent compared to the positive control Quercetin.

Test Example  2. Cytotoxicity Assessment

In order to measure the cytotoxicity of the active extract prepared in Example 1 rat-derived macrophage (Raw264.7) inoculated in a 96 well plate at a concentration of 1 x 10 ⁵ cells / ml and incubated in Example 1 The prepared active extract was diluted to 10, 20, 50, 100, 200 µg / ml concentration using serum-free medium, and then treated for 24 hours, and the survival rate of the cells was evaluated using MTT reagent. At this time, serum-free medium was used as a control.

division Concentration (㎍ / ml) Cell viability (%) Control group 100.00 ± 5.29 Example 1 10 126.56 ± 8.62 20 139.63 ± 16.44 50 111.11 ± 5.10 100 71.77 ± 2.69

As a result of Table 2, the active extracts prepared in Example 1 did not show toxicity when the cells were treated up to a concentration of 10-50 µg / ml, and the test group treated with the highest concentration of 100 µg / ml. Showed about 30% toxicity.

Test Example  3. Nitric oxide  Generation inhibitory effect

In order to measure the inhibitory effect of nitric oxide production of the active extracts prepared in Example 1, rat-derived macrophages (Raw264.7) were inoculated in 24 well plates at a concentration of 5 x 10 ⁵ cells / ml. Incubated for 24 hours at 5% CO ₂ conditions of 37 ℃. In order to apply the active extract prepared in Example 1 to the cultured cells, the cells were stimulated with LPS (Lipopolysaccharide), which is known to induce the production of nitric oxide, was diluted to a concentration of 10 ~ 200㎍ / ml . As a positive control, N-methyl-L-arginine acetate salt (L-NMMA) was used, and the control group was treated with medium containing only LPS. Cells were harvested for 24 hours, centrifuged at 12,000 rpm for 10 minutes, the supernatant was taken and reacted with the same amount of Griess reagent, and then absorbance was measured at 540 nm.

division Concentration (㎍ / ml) NO generation inhibition (%) Control group 84.32 ± 0.69 Example 1 10 24.65 ± 0.68 50 60.29 ± 0.19 100 81.77 ± 0.42

As shown in Table 3, in the case of the active extract prepared in Example 1 of the present invention, the effect of inhibiting the production of LPS-induced nitric oxide concentration-dependently was confirmed, the IC 50 is about 29.25㎍ / ml Was measured. In this experimental example, it was confirmed that the inhibitory effect of nitric oxide production of the active extract, which shows the anti-inflammatory effect at the cellular level.

Test Example  4. Evaluation of skin irritation

In order to evaluate the skin irritation of the active extracts prepared in Example 1, a primary patch test and a cumulative stimulation test were performed on a human body through a human patch test.

Twenty healthy men and women were tested according to CTFA guidelines (The Cosmetic, Toiletry and Fragrance Association. Inc. Washington, D.C., 20036, 1991). 20 μl of the sample was added dropwise to a Fin chamber, which was then patched on one side of the arm of the human body, which was the test site, and fixed with tape.

In the first skin irritation test, the patch was removed for 24 hours, the patch was removed, and then the skin reaction of the test site after 4 hours and 24 hours was determined according to the criteria of Table 4 below.

The Repeated Insult Patch Test (RIPT) is a three-week routine skin exposure followed by a second closed challenge test on new areas two weeks later to detect irritation and allergens on the skin. As a test to observe, 10 μl of a patient without a history of skin disease was added dropwise to the Finn Chamber in Example 1 at a concentration of 10 mg / ml, and then fixed with Scanpor tape inside both arms as test sites. After 24 hours of application, the patch was removed, and a total of 9 repetitive applications were performed on the same site at the same schedule at 24 hour intervals. The degree of skin irritation was evaluated visually based on the primary skin irritation evaluation criteria, and a scoring scale list was prepared.

sample Number of test subjects Judgment result Irritation degree ++ + ± - Test group 20 - - - 20 0 Control group 20 - - - 20 0 (Criteria)
-: No erythema or unusual phenomenon
±: slightly reddish
+: Significantly redder than the surroundings
++: reddens and swells more heavily than the surroundings
(Stimulus calculation formula)
Stimulus = [{(±) Number × 1} + {(+) Number × 2} + {(++) Number × 3}] / Number of Subjects

sample Number of test subjects Induction stage Challenge stage Irritation degree 20 One 2 3 4 5 6 7 8 9 24hr 48hr 72hr Test group 20 - - - - - - - - - - - - 0 Control group - - - - - - - - - - - - 0
(formula)
Stimulus = [{(±) Number × 1} + {(+) Number × 2} + {(++) Number × 3}] / Number of Subjects

As can be seen from the results of Table 4 and Table 5, as a result of evaluating the skin irritation test on the human body, the activation extract prepared in Example 1 of the present invention does not cause irritation and confirmed that the material is safe to apply to the skin Could.

Formulation example  1. Formulation of Cosmetics

Cosmetic composition containing the active extract of the present invention can be used in a variety of cosmetics and cleansing agents for acne skin, the dosage form can be arbitrarily selected. Products to which the composition can be added include cosmetics such as creams, milk lotions, foundations, skins, etc., which are external preparations applied to various skins, and cleansing foams, face washes, soaps, treatments, and essences.

The following shows a specific formulation example of the cosmetic composition containing the kkujippung tree extract of the present invention, the formulation containing the cosmetic composition of the present invention is not limited thereto.

Formulation of the skin toner in the cosmetic containing the active extract of Example 1 is as follows.

Compounding ingredient Content (% by weight) Example 2 Carboxyvinyl Polymer 3 Butylene glycol 4 Propylene glycol 3 Methylphenylpolysiloxane 0.1 Methyl paraben 0.1 ethyl alcohol 2 Triethanolamine 0.04 EDTA-2Na 0.02 Purified water to 100

The formulation of the lotion (emulsion) in the cosmetic containing the active extract of Example 1 is as follows.

Compounding ingredient Content (% by weight) Example 2 Carboxyvinyl Polymer 4 Ethyl hydrobenzoate 0.1 Liquid paraffin 3 Vitamin E Acetate 0.1 silicon 0.8 Ketyl Alcohol 0.5 Sorbitan monostearate One Polysorbate 60 0.3 Methylsulfonylfetan 0.2 Propylene glycol 2 Butylene glycol 5 Methyl paraben 0.2 Triethanolamine 0.1 EDTA-2Na 0.03 Purified water to 100

Formulation of the essence in the cosmetic composition containing the active extract of Example 1 is as follows.

Compounding ingredient Content (% by weight) Example 2 Carboxyvinyl Polymer 15 glycerin 6 Propylene glycol 3 Macadamia oil 0.5 Methyl paraben 0.1 ethyl alcohol 2 Triethanolamine 0.15 EDTA-2Na 0.02 Purified water to 100

Claims (3)

Active extract for skin aging extracted by adding water, hydrophilic organic solvent or mixed solvent thereof to the active
The method of claim 1,
The active extract is a skin anti-aging cosmetic composition, characterized in that it contains active extract and active ingredient in the active
The method of claim 1,
The active extract is a skin aging cosmetic composition, characterized in that it exhibits anti-inflammatory and antioxidant effects

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