KR20140064280A - Somniferous composition comprising the extracts, fractions or isolated compounds of lettuce and process for preparation thereof - Google Patents

Abstract

Disclosed is a somniferous composition comprising an extract of lettuce or a fraction of lettuce as an active ingredient. Also, disclosed is a preparation method of the somniferous composition which includes the steps of: extracting lettuce using a solvent of which weight is 1-20 times of weight of the lettuce at 50°C-100°C for10-60 minutes; filtering the extracted solution of lettuce; and concentrating the filtered solution under reduced pressure to obtain an extract of lettuce. The extract of lettuce, a fraction of lettuce, or a compound isolated from the same has a somniferous effect; is derived from natural substances and thus is safe; and can be advantageously used as an active ingredient of a food composition for inducing sleep or alleviating sleep disorders.

Description

TECHNICAL FIELD The present invention relates to a composition for inducing sleep and a composition for preparing the same, and a method for producing the same, which comprises extracting lettuce, lettuce fractions or compounds isolated therefrom,

The present invention relates to a water-surface-inducing composition containing lettuce extract.

Sleep is defined as a state in which the ability to recognize and react to the external environment is reversible, repetitive, and normally stationary, although it can be defined in many ways. Sleep is caused by brain activity, but is also deeply related to physiological changes in other parts of the body.

Sleep disorders are a very common disorder in which more than 20% of the population have experienced or are suffering. Sleep disturbances can cause a variety of personal and social problems and can be causes of learning disability, inefficiency, traffic accidents, safety accidents, emotional disturbances, social adjustment disorders, dissatisfaction with marriage, and industrial accidents. In addition, if the sleep disorder is not properly treated, the medical, neurological, and psychiatric illnesses that are already suffering may deteriorate or the recovery may be delayed, and serious diseases such as myocardial infarction and stroke may result.

To treat sleep disorders, a chemical sleeping agent is used to reversibly depress the central nervous system to induce sleep and maintain a sleeping state. Conventional sleeping pills have a central inhibitory action such as anesthetics, so that a small amount of sedative action, while a moderate amount of sleep action, but a large amount of coma, paralysis, respiratory depressant action. Barbituric drugs have low safety and resistance and dependence easily occur, and there is a problem that sleep disorder caused by nightmares is caused when the drug is stopped after a long term use.

Recently, benzodiazepine medicines with fewer harmful effects and anti-anxiety effects have been used, but the problem of dependency has not been solved, and excessive use of medicines such as helminth, coma, coronary vasodilation, May cause congenital anomalies.

The present invention provides a water-surface-inducing composition, a water-surface-inducing agent, a food composition, and a method for producing the water-surface-inducing composition containing extracts of lettuce, lettuce fractions or compounds isolated therefrom as an active ingredient.

The present invention provides a water-surface-inducing composition containing an extract of lettuce or a fraction of lettuce as an active ingredient.

In addition, the present invention provides a water-surface-inducing composition containing, as an active ingredient, a lettuce extract or a lettuce fraction extracted from lettuce on which a flower bud is raised.

The present invention also provides a water-surface-inducing composition comprising, as an active ingredient, a lettuce extract or a lettuce fraction extracted from a flower bud of lettuce.

The present invention also provides a water-insoluble composition comprising lettuce extract or lettuce fraction extracted from one or more mixed solvents selected from the group consisting of water, C1-C4 alcohols and acetone as an active ingredient.

In addition, a group consisting of? -Lactucerol,? -Lactucerol, Lactucin, Lactucopicrin and a pharmaceutically acceptable salt thereof separated from lettuce As an active ingredient, is provided.

In addition, the present invention provides a water-surface-inducing composition comprising lettuce extract, lettuce fraction or a compound isolated therefrom in an amount of 0.1 to 50% by weight based on the total weight of the composition.

In addition, the present invention provides a water surface directing agent further comprising a pharmaceutically acceptable carrier in the water-insoluble composition.

In addition, the present invention provides a food composition further comprising a food-acceptable additive in the water-insoluble composition.

The present invention also relates to a method for producing a water-insoluble composition comprising extracting lettuce with a solvent at 1 to 20 times the weight of lettuce at 50 to 100 DEG C for 10 to 60 minutes, and then filtering and concentrating the solution under reduced pressure to obtain an extract of lettuce .

The present invention also provides a method for producing a water-surface-inducing composition, wherein the solvent is one or more mixed solvents selected from the group consisting of water, C1-C4 alcohols and acetone.

The present invention also provides a method for preparing a water-insoluble composition, which comprises suspending the extract of lettuce in water and sequentially fractionating the extract with normal-hexane, methylene chloride, ethyl acetate, n-butanol and water to obtain a lettuce fraction .

Also, the present invention provides a method for preparing a water-insoluble composition, wherein the lettuce fraction is one selected from the group consisting of a normal-hexane fraction, a methylene chloride fraction, an ethyl acetate fraction, a normal-butanol fraction and a water fraction .

The lettuce extract, lettuce fractions or compounds isolated therefrom of the present invention exhibit a water-inducing effect and are safe substances derived from natural materials, and thus can be usefully used as an effective ingredient of a food composition for inducing sleep or improving sleeping disorders.

Fig. 1 is a photograph of lettuce on which a pedicel is used, which is used in an embodiment of the present invention.
2 is a photograph of the lettuce powder obtained by the method of Production Example 2-1 of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention is capable of various modifications and various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. It is to be understood, however, that the invention is not to be limited to the specific embodiments, but includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

One embodiment of the present invention provides a composition having a water-inducing action containing an extract of Lettuce, a lettuce fraction or a compound isolated therefrom as an active ingredient.

Further, a modification of the embodiment of the present invention provides a composition having a water-inducing action containing, as an active ingredient, a lettuce extract or a lettuce fraction extracted from lettuce raised with a flower bud.

Further, another modified example of the embodiment of the present invention provides a composition having a water-inducing action containing an extract of lettuce extracted from lettuce or an extract of lettuce as an active ingredient.

Lettuce is a perennial herbaceous plant of the dicotyledonous plant, and is widely grown in Europe and West Asia as a vegetable. The stem is very branched and has a height of 90 to 120 centimeters, with no hairs on the whole. Leaves from the root are elliptical and large, and the largest leaves are 20-30 cm long.

Lettuce is mainly used for chewing salads and wrappers, and it is eaten as an eggplant. Vitamins and minerals are abundant and are known to be good for anemic patients. On the other hand, it is common to eat only lettuce leaves when eating lettuce, and it is common to discard the roots, stems, and flower buds of lettuce.

Lettuce grows according to varieties such as lettuce lettuce, lettuce, cabbage lettuce, and lettuce. As shown in Fig. 1, after harvesting, the bitter taste of lettuce becomes stronger.

The flower buds of lettuce are not bitter and bitter. The leaf of the lettuce which the flower stalk is raised is not used for food because it is bitter, and it is common to harvest only the lettuce leaf before the flower stalk is raised. Traditionally, the peduncles of lettuce and lettuce on which the pedicles were raised were discarded.

The lettuce used in an embodiment and a variation of the present invention includes lettuce or lettuce or lettuce raised in a flower bud, and is used in a variety of forms such as a lollaroose, an undiluted facet, an aphrodite facet, an acuplicate, a flame facet, It is not limited to breed of lettuce such as red skirt,

The water-surface-inducing composition comprising lettuce extract, lettuce fractions or compounds isolated therefrom of the present invention is preferably prepared by the following steps, but is not limited thereto.

The method for preparing the water-surface-inducing composition comprises: extracting lettuce by adding a solvent; Filtrating and concentrating the extract to obtain an extract of lettuce; And optionally, suspending the produced extract in water and fractionating it with a solvent to obtain an lettuce fraction.

In the above production method, lettuce may be cultivated, harvested, or marketed without limitation. The lettuce may be a leaf, a stem or a root, but is not limited thereto.

In one embodiment of the invention, the solvent in the extraction step is one or more than one selected from the group consisting of water, alcohol, acetone, or a mixture thereof, preferably water, a C ₁ to C ₄ alcohol and acetone A mixed solvent, more preferably an aqueous solution of methanol, ethanol or acetone, but is not limited thereto.

In one embodiment of the present invention, the amount of the extraction solvent is preferably 1 to 20 times, more preferably 10 times, the dry weight of the lettuce, but is not limited thereto. When the amount of the solvent is less than 1 times, the solvent does not play a sufficient role. When the amount of the solvent is more than 20 times, the extraction degree is similar.

The extracts of lettuce or lettuce, which can be used in the composition for inducing sleep in the present invention, are suitably from 1 to 50% by weight, based on the weight of the respective lettuce extract or fraction, of the solid content excluding the solvent (or all the remaining ingredients except for the solvent).

The extraction method may be selected from the group consisting of supercritical extraction, subcritical extraction, high temperature extraction, high pressure extraction, hot water extraction, immersion extraction, adsorption resin including XAD and HP-20, reflux temperature extraction, , And preferably an ultrasonic extraction method, a reflux temperature extraction, and a room temperature extraction may be used, but the present invention is not limited thereto.

The number of extraction times is preferably 1 to 5 times, more preferably 3 times, but is not limited thereto. When the number of times of extraction is more than 5 times, there is no change in extraction degree.

The temperature at the time of extraction may preferably be 50 ° C to 100 ° C. If the temperature is lower than 50 ° C, the extraction efficiency is lowered. If the temperature is higher than 100 ° C, the extraction degree is not changed. For extraction of a certain component, extraction is preferably carried out at 100 ° C. in a solvent containing pure water, but not limited thereto.

The extraction time is preferably 10 min to 60 min, more preferably about 30 min, but is not limited thereto. In case of less than 10 minutes, the extraction efficiency is lowered.

In an embodiment of the present invention, it is preferable to use a vacuum rotary evaporator in the step of obtaining the extract of lettuce, but the present invention is not limited thereto. In addition, the extract may further be dried, and the drying may be carried out under reduced pressure, vacuum drying, boiling, spray drying, room temperature drying or freeze drying, but is not limited thereto.

In one embodiment of the present invention, the solvent in the step of obtaining the lettuce fraction may be an organic solvent, preferably, but not limited to, n-hexane, methylene chloride, ethyl acetate or n-butanol. The lettuce fraction may be obtained by suspending an extract of lettuce in water and sequentially fractionating the fraction with normal-hexane, methylene chloride, ethyl acetate, n-butanol and water, and preferably fractionated with normal-hexane fraction, methylene chloride fraction, ethyl Acetate fraction, n-butanol fraction and water fraction, more preferably ethyl acetate fraction, but is not limited thereto. The lettuce fraction can be obtained from the lettuce extract by repeating the fractionation process 1 to 5 times, preferably 3 times, preferably after fractionation, but it is not limited thereto.

One embodiment of the present invention is a pharmaceutical composition comprising α-Lactucerol (Taraxasterol), β-lactucerol (Lactucon, Lactucerin) represented by Chemical Formula 2, There is provided a water-surface-inducing composition comprising, as an active ingredient, at least one selected from the group consisting of Lactucin, Lactucopicrin represented by the formula (4) and a pharmaceutically acceptable salt thereof.

The term " pharmaceutically acceptable salt " means salts having pharmaceutically acceptable and desired pharmacological activity as defined above. Such salts include organic and inorganic acids such as, for example, hydrogen chloride, hydrogen bromide, hydrogen iodide, sulfuric acid, phosphoric acid, acetic acid, glycolic acid, maleic acid, malonic acid, oxalic acid, toluenesulfonic acid, methanesulfonic acid, trifluoroacetic acid, fumaric acid , Succinic acid, tartaric acid, citric acid, benzoic acid, ascorbic acid, and the like.

The water-insoluble composition of the present invention preferably contains the extract of lettuce extract, lettuce fraction or the compound isolated therefrom in an amount of 0.1 to 50% by weight based on the total weight of the composition, but is not limited thereto.

One embodiment of the present invention provides a sleep inducing agent further comprising a pharmaceutically acceptable carrier in the water-insoluble composition.

In one embodiment of the present invention, the water surface inducing agent may further comprise a suitable pharmaceutically acceptable excipient or diluent.

The water surface-inducing agent of the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols or the like, oral preparations, suppositories and sterilized injection solutions according to a conventional method have.

Examples of the carrier, excipient or diluent that can be included in the water surface inducing agent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

 In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used. Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose, Lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included . Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of suppository bases include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like.

The sleep-inducing agent of the present invention can be administered orally or parenterally (for example, intravenously, subcutaneously, intraperitoneally or topically) according to a desired method, and the dose is determined depending on the condition of the patient, The dosage, the excretion rate, the severity of the disease, the drug form, the administration time, the administration method, the administration route, and the administration period. The daily dose is 100 mg / kg to 300 mg / kg, preferably 100 mg / kg to 200 mg / kg, when the extract, fraction or compound according to the present invention is lyophilized, It may be administered once or several times a day.

One embodiment of the present invention provides a food composition further comprising a pharmaceutically acceptable additive in the water-insoluble composition. The food composition is provided as a food composition for prevention and improvement of fatigue.

The food composition includes all forms of functional food, nutritinal supplement, health food and food additives. Food compositions of this type may be added as such or used with other food or food ingredients, and may be used variously according to conventional methods known in the art.

Examples of the food composition to which the above substance can be added include dairy products including dairy products such as drinks, meat, sausage, bread, biscuits, rice cakes, candies, snacks, confectionery, pizza, ramen noodles, , Beverages, alcoholic beverages, and vitamin complexes, all of which include foods in a conventional sense.

The lettuce extract, lettuce fraction or compounds isolated therefrom of the present invention can be added directly to food or used together with other food or food ingredients, and can be suitably used according to conventional methods. The amount of the active ingredient to be mixed can be suitably determined according to its use purpose (for prevention or improvement). Generally, the amount of the extract in the food composition may be 0.1 to 90 parts by weight of the total food. However, in the case of long-term intake intended for health and hygiene purposes or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

When the food composition of the present invention is a health functional beverage, there are no particular restrictions on other components other than those containing the above-mentioned extract as essential components in the indicated ratios, and various flavors or natural carbohydrates, can do. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose, etc .; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 g of the composition of the present invention.

In addition to the above, the extract of Lettuce, the fraction of lettuce, or the compound isolated therefrom of the present invention may contain flavors such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and intermediates (cheese, , A pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated beverages and the like. In addition, the lettuce extract or lettuce fraction of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks. These components may be used independently or in combination. Although the ratio of such additives is not so important, it is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the lettuce extract or lettuce fraction of the present invention.

Hereinafter, the present invention will be described in detail with reference to Examples, Experimental Examples and Preparation Examples.

EXAMPLES The following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by the following Examples, Experimental Examples and Preparation Examples.

 ≪ Example 1 > Preparation of mixed lettuce extract

The lettuce with the peduncle was crushed to an appropriate size. 6 kg of lettuce and 6 liters of ethanol solution were added to the extraction container, and the mixture was allowed to stand at room temperature for 7 days, and then filtered with filter paper to obtain an extract. The extraction procedure was repeated three times, and then the solvent was concentrated under reduced pressure to obtain 60 g of an extract.

<Example 2> Preparation of mixed extract of lettuce

A mixed-solvent extract of lettuce was obtained by the same method as in Example 1, except that lettuce was replaced by lettuce instead of lettuce grown up.

<Example 3> Preparation of hot water extract of lettuce

1400 ml of water at 50 ° C to 100 ° C was added to 6000g of lettuce, and the extraction was repeated three times. In the extraction process, water was added to extract 18000 ml of lettuce hot water extract in the process of extracting water temperature. The resulting extract of hot water of lettuce was filtered 2 ~ 3 times to remove the by-products, and then 90 mL of the extract was placed in 200 pouches.

Example 4: Preparation of hot water extract of lettuce flower

A hot-water extract of lettuce was obtained by the same method as in Example 3, except that lettuce flowers were used instead of lettuce flowers.

Experimental Example 1 Measurement and Comparison of Water Quality

The quality of sleep was measured to verify the efficacy of the present invention. The Pittsburgh Sleep Quality Index (PSQI) was used to measure sleep quality. The evaluation items of PSQI are shown in <Table 1> below. The questionnaire was revised by evaluating the quality of sleep for 7 days. Fill out the questionnaire twice per person, fill in the lettuce before taking it, take the lettuce for 7 days, and re-fill the same questionnaire.

A zero in each item indicates that there is no difficulty. Three points represent serious difficulties. Seven component scores are added to create one overall score, with a score range of 0 to 21. A score of 0 indicates that there is no difficulty, and a score of 21 indicates that there is a serious difficulty across the front.

Evaluation items Questionnaire contents 1. subjective sleep quality Self-rated sleep quality 2. Sleep latency Time to sleep,
The number of times you did not get well within 30 minutes of a week 3. Sleep period What actually sleeps for a few hours? 4. Habitual sleep effect (Actual sleep time) / (number of hours spent in the bedroom) × 100 = habitual sleep efficiency (%) 5. Sleep disturbance The number of 9 sleep interferences (number of breaks in the middle, frequency of going to the bathroom, breathing inconvenience, snoring, cold, fever, nightmares, pain, etc) 6. Sleep medication use The number of times the medication was taken to sleep 7. Daytime dysfunction The number of times you are awake and have problems,
Number of times you have problems with enough motivation

Statistical package program SPSS 13.0 was used to analyze the collected data. Repeated Measures ANOVA of the General Linear Model was applied for the pre - post test of the experimental group and the control group. p-value) was less than 0.05 was considered statistically significant.

In this experiment, 26 experimental groups and 23 control groups participated. Twenty - six of the experimental groups were divided into two questionnaires and seven bars of lettuce hot water extract (90 ml extracted from lettuce 30 g) obtained by the method of Example 3. 30g of lettuce contained in 90ml of lettuce hot water extract is a daily dose of herbal medicine which is not strong in Oriental medicine, and it is dose once per day per person. In the control group, 2 questionnaires and 7 roots of hydrolyzate extract (90ml extracted from 0.1g of Sukjipgang) were distributed. The experiment was carried out by filling out the questionnaire before taking, taking one bag after dinner every day, filling out 7 bars for 7 days, and then filling out the questionnaire again.

To 90 ml of water was added 0.1 g of steamed rice ginger and the boiled water was used as a control for the lettuce hot water extract. The reason for selecting Sukjihwanghwang is that it can produce color and aroma similar to that of lettuce hot water extract even in a small amount. 0.1 g of Sulfur Sulfur has little effect on the experiment. The control group also provides the same questionnaire as the experimental group, and the subjects do not know that they belong to the control group.

An independent sample t-test was used for the experimental group and the control group to verify that the experimental group and the control group had the same composition. As shown in <Table 2>, there was no significant difference between the experimental group and the control group, and the experimental group and the control group used in this analysis were the same group. In Table 2, M is the mean of the total PSQI scores, and SD is the standard deviation. t is the test value of the t-test, and p is the significance probability.

Control group Experimental group t p M SD M SD Before experiment Overall PSQI score 12.35 4.91 12.42 4.17 0.003 0.954

After the end of the experiment, the questionnaire results were analyzed. <Table 3> shows the overall PSQI score of the control group and the experimental group (taking the extract of lettuce) after the end of the experiment. The overall PSQI score is the sum of all 7 items. As shown in Table 3, significant differences can be found between the control and experimental groups. Although the efficacy of the psychological effects was partially confirmed in the control group of Table 3, it can be seen that the experimental group had a greater effect on the induction of sleep. As shown in Table 3, the significance (p value, p) of the control group was 0.005. As shown in Table 4, the p value was 0.000 in the experimental group. In the table, DM (Delta M) means the difference between the pre-M value and the post-M value.

PSQI score M SD Pre-post t p DM SD Control group dictionary 12.35 4.91 0.70
1.06
3.138
0.005
Death 11.65 4.64 Experimental group dictionary 12.42 4.17 2.23
1.97
5.786
0.000
Death 10.19 4.02

Experimental Example 2: Comparison of subjective sleep quality

The subjective quality of sleep in PSQI is a score that patients themselves evaluate for their sleep. As shown in <Table 4>, pre-post-test scores of subjective sleep quality were not effective in the control group (p = 0.056) and showed efficacy in the experimental group. (p = 0.003)

M SD Pre-post t p DM SD Control group dictionary 2.04 0.88 0.26 0.62 2.021 0.056 Death 1.78 0.90 Experimental group dictionary 2.00 0.69 0.46 0.71 3.333 0.003 Death 1.54 0.91

<Experimental Example 3> Comparison of sleep latency

In PSQI, sleep latency is a measure of the time it takes to sleep and the number of times that it has not slept within 30 minutes of the last week. As shown in <Table 5>, the control group had no efficacy (p = 0.057) and the efficacy was in the experimental group. (p = 0.008)

M SD Pre-post t p DM SD Control group dictionary 2.04 0.71 0.22 0.52 2.011 0.057 Death 1.83 0.89 Experimental group dictionary 2.12 0.65 0.46 0.81 2.900 0.008 Death 1.65 0.80

<Experimental Example 4> Comparison of sleeping periods

In PSQI, sleep duration is a measure of how many hours you actually sleep at night. As shown in <Table 6>, the pre-post test of the sleeping time score showed no efficacy in the control group (p = 0.162) and was effective in the experimental group. (p = 0.029)

M SD Pre-post t p DM SD Control group dictionary 1.96 0.98 0.09 0.29 1.447 0.162 Death 1.87 0.92 Experimental group dictionary 1.77 1.14 0.31 0.68 2.309 0.029 Death 1.46 0.95

Experimental Example 5 Comparison of habitual sleeping effect

In PSQI (actual sleep time / time spent in the bedroom) × 100 = habitual sleep efficiency, the value of the habitual sleep effect score is obtained. As shown in Table 7, there was no effect in the control group (p = 0.328) and the effect was in the experimental group. (p = 0.031)

M SD Pre-post t p DM SD Control group dictionary 1.70 1.06 0.04 0.21 1,000 0.328 Death 1.65 1.11 Experimental group dictionary 1.65 1.09 0.23 0.51 2.287 0.031 Death 1.42 0.90

Experimental Example 6 Comparison of Sleep Interference

In PSQI, sleep disturbance is a score that evaluates the number of times that symptoms interfere with nine sleeping conditions. As shown in <Table 8>, pre-post test of sleep disturbance score showed no efficacy in the control group (p = 0.083) and no efficacy in the experimental group. (0.161)

M SD Pre-post t p DM SD Control group dictionary 1.57 0.79 0.13 0.34 1.817 0.083 Death 1.43 0.79 Experimental group dictionary 1.54 0.81 0.08 0.27 1.443 0.161 Death 1.46 0.81

<Experimental Example 7> Comparison of sleeping drug use

In PSQI, sleep drug use is a measure of the number of times a drug has been taken to sleep. As shown in <Table 9>, pre-post test of sleep drug use showed no efficacy in the control group (p = 0.328) and no efficacy in the experimental group. (p = 0.103)

M SD Pre-post t p DM SD Control group dictionary 1.13 1.39 0.04 0.21 1,000 0.328 Death 1.09 1.35 Experimental group dictionary 1.23 1.42 0.15 0.46 1.690 0.103 Death 1.08 1.29

<Experimental Example 8> Comparison of daytime dysfunction

In PSQI, daytime dysfunction is the number of times it is difficult to maintain arousal during the day and the score of whether or not it has enough motivation to work. As shown in <Table 10>, pre-post test of daytime dysfunction showed no efficacy in the control group (p = 0.328) and efficacy in the experimental group. (p = 0.002)

M SD Pre-post t p DM SD Control group dictionary 1.96 1.15 -0.04 0.21 -1,000 0.328 Death 2.00 1.17 Experimental group dictionary 2.12 0.99 0.54 0.81 3.384 0.002 Death 1.58 0.86

 &Lt; Preparation Example 1 > Preparation of pharmaceutical preparations

&Lt; Preparation Example 1-1 >

2 g of the lettuce extract of Example 1 or Example 2 and 1 g of lactose were mixed and filled in airtight bags to prepare powders.

&Lt; Preparation Example 1-2 > Preparation of tablet

100 mg of the lettuce extract of Example 1 or Example 2, 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate were mixed and then tableted according to a conventional method for preparing tablets.

&Lt; Preparation Example 1-3 > Preparation of capsules

100 mg of the lettuce extract of Example 1 or Example 2, 100 mg of corn starch, 100 mg of milk sugar and 2 mg of magnesium stearate were mixed and filled in gelatin capsules according to the conventional preparation method of capsules to prepare capsules.

&Lt; Preparation Example 1-4 >

1 g of the lettuce extract of Example 1 or Example 2, 1.5 g of lactose, 1 g of glycerin, and 0.5 g of xylitol were mixed to prepare 4 g per one ring according to a conventional method.

<Preparation Example 1-5> Preparation of granules

150 g of the lettuce extract of Example 1 or Example 2, 50 mg of soybean extract, 200 mg of glucose and 600 mg of starch were mixed, and then 100 mg of 30% ethanol was added and dried at 60 ° C to form granules.

&Lt; Preparation Example 2 > Production of food

The acid, the tablet, the capsule, the ring and the granule of Preparation Example 1 may be applied to food. The food containing the above-mentioned lettuce extract was prepared as follows.

<Production example 2-1> Preparation of powder

The lettuce extracts of Example 1 or Example 2 were dried by a known method together with brown rice, barley, glutinous rice, and yulmu and dried to prepare mixed powders having a particle size of 60 mesh or less by a pulverizer. Depending on the preference, black soybeans, black sesame seeds and perilla seeds may be steamed and dried, and then they may be added to the mixed powder after being made into powder having a particle size of 60 mesh by a pulverizer. The mixed powder may be diluted with water to be ingested as a raw or powdered powder.

&Lt; Preparation Example 3 > Preparation of beverage

A drink containing lettuce extract was prepared as follows.

<Production example 3-1> Preparation of health drink

(0.5 parts by weight), oligosaccharide (2 parts by weight), sugar (2 parts by weight), salt (0.5 part by weight) and water (75 parts by weight) were added to the lettuce extract of Example 3 or Example 4 The extract of lettuce (0.5 part by weight) was uniformly blended and instant sterilized, and then packaged in a glass bottle, plastic bottles, and plastic bottles to prepare health drinks.

<Production example 3-2> Preparation of vegetable juice

100 ml of the lettuce extract of Example 3 or Example 4 was added to 1,000 ml of vegetable juice such as tomato or carrot to prepare a vegetable juice for health promotion by a conventional method.

&Lt; Preparation Example 3-3 > Preparation of fruit juice

100 ml of the lettuce extract of Example 3 or Example 4 was added to 1,000 ml of fruit juice such as apple or grape, and fruit juice for health promotion was prepared by a conventional method.

Although the composition ratio is relatively mixed with the ingredients suitable for food, it is also possible to arbitrarily modify the compounding ratio according to the regional and national preferences such as the demand level, the demanding country, and the use purpose.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. Accordingly, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (12)

A composition for inducing sleep in a water comprising an extract of lettuce or a fraction of lettuce as an active ingredient.
The method according to claim 1,
Wherein the extract of lettuce or the fraction of lettuce is extracted from lettuce grown on a flower bud.
The method according to claim 1,
Wherein the extract of lettuce or the fraction of lettuce is extracted from the flower bud of lettuce.
The method according to claim 1,
Wherein the extract is obtained by extracting lettuce with one solvent selected from the group consisting of water, C1-C4 alcohol and acetone, or two or more mixed solvents.
Lactucerol, β-Lactucerol, Lactucin, Lactucopicrin, and pharmaceutically acceptable salts thereof, isolated from lettuce, selected from the group consisting of α-Lactucerol, β-Lactucerol, Or a pharmaceutically acceptable salt thereof as an active ingredient.
6. The method according to any one of claims 1 to 5,
Wherein the extract of lettuce, the lettuce fraction or the compound isolated therefrom is contained in an amount of 0.1 to 50% by weight based on the total weight of the composition.
6. A composition for sleeping in a sleeping condition, which composition further comprises a pharmaceutically acceptable carrier in the composition of any one of claims 1 to 5.
A food composition comprising a composition according to any one of claims 1 to 5 and further comprising a pharmaceutically acceptable additive.
Extracting the lettuce with a solvent of 1 to 20 times the weight of the lettuce at 50 to 100 DEG C for 10 to 60 minutes, filtering and concentrating the solution under reduced pressure to obtain an extract of lettuce.
10. The method of claim 9,
Wherein the solvent is one solvent selected from the group consisting of water, C1 to C4 alcohols and acetone, or a mixture of two or more solvents.
10. The method of claim 9,
Further comprising the step of suspending the extract of lettuce in water and sequentially fractionating it with n-hexane, methylene chloride, ethyl acetate, n-butanol and water to obtain a lettuce fraction.
10. The method of claim 9, wherein the lettuce fraction is one selected from the group consisting of a normal-hexane fraction, a methylene chloride fraction, an ethyl acetate fraction, a normal-butanol fraction, and a water fraction.

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