KR20140041635A - Composition for protecting skin and inhibiting damage of skin against uv comprising polysiphonia morrowii harvey - Google Patents

Abstract

The present invention relates to a pharmaceutical composition comprising Polysiphoniai morrowii Harvey extract as an active ingredient for treating skin and inhibiting skin damage caused by ultraviolet. The Polysiphonia morrowii Harvey extract of the present invention has an outstanding antioxidative effect through an activity of eliminating a free radical in cells generated by ultraviolet irradiation, and also the Polysiphonia morrowii Harvey extract has outstanding effects of directly absorbing ultraviolet per se and inhibiting apoptosis induced by ultraviolet. Thus, the pharmaceutical composition of the present invention, which comprises the Polysiphonia morrowii Harvey extract as an active ingredient, can be usefully applied to skin treatment and skin protection by effectively protecting skin cells from ultraviolet. Particularly, since the Polysiphonia morrowii Harvey extract is a native substance, which is an edible material, the composition of the present invention comprising the Polysiphonia morrowii Harvey extract as an active ingredient is beneficial to long term use in terms of safety.

Description

Composition for protecting skin and inhibiting damage of skin against UV Comprising Polysiphonia morrowii Harvey}

The present invention relates to a therapeutic pharmaceutical composition and a composition for absorbing ultraviolet rays that can inhibit skin damage caused by ultraviolet rays and protect the skin.

In general, ultraviolet rays contained in sunlight can cause erythema formation or melanin pigmentation in skin cells when excessively directly irradiated to the skin, which can cause blemishes and blemishes, and react with sebum secreted by the epidermis and peroxidation. The production of lipids can cause skin problems and, in severe cases, can cause skin cancer. Ultraviolet rays are classified into UV-A (320 to 400 nm), UV-B (280 to 320 nm) and UV-C (200 to 280 nm) depending on the wavelength. Among them, ultraviolet Are known as UV-A and UV-B.

UV exposure increases free radical formation and the generation of reactive oxygen species (ROS) in the skin, and induces aging stress by oxidative stress on cellular components such as DNA, membranes and proteins. It is known to cause.

The sunscreens used for the purpose of preventing skin damage by ultraviolet rays are classified into chemical sunscreens and physical sunscreens.

Chemical blocking agents that act as chemical absorption of ultraviolet rays are well known organic blocking agents such as cinnamic acid, salicylic acid and benzophenone. Blockers are well known. Chemical blockers have the advantage of excellent UV-blocking effect, but has a disadvantage of causing skin irritation because it is narrow in the wavelength range applied, and is applied to the cosmetic in a sticky and bundley feeling and molecular form when applied to cosmetics. In the case of physical blockers, skin irritation is relatively low compared to chemical blockers but has the disadvantage of causing heavy feeling of use, whitening and phototoxicity.

In order to compensate for the above disadvantages of the chemical and physical blockers, various studies on sunscreens have been conducted, and in particular, studies on natural sunscreen ingredients having advantages in skin safety have been actively conducted. As a technology developed in this regard, Korean Patent Nos. 10-0526637, 10-0858630, 10-0114124, and 10-0162282, etc., contain natural ingredients extracted from drawing, green beans, coffee, pansy, rhubarb, etc. A sunscreen cosmetic composition has been developed.

Although the above-described techniques include natural sunscreen ingredients having excellent skin safety, the direct sunscreen effect is low, and thus has a disadvantage of using a mixture of conventional synthetic organic sunscreen ingredients and natural extracts instead of a single natural extract.

In addition, as a technology developed in connection with the above, US Patent No. 5552135 developed a sunscreen natural ingredient containing ferulic acid (ferulic acid) and ethyl ferulate (ethylferulate) in grains such as oats, corn, wheat, rice, In addition, the present invention proposes a technique of increasing the sunscreen effect when used in combination with an organic sunscreen ingredient rather than a single ingredient.

On the other hand, Morrow red thread ( Polysiphonia morrowii Harvey) is a red algae in seawater, belongs to the Rhodomelaceae. It is reported to be distributed in Jeju Island and coastal areas of Korea, and in Asia such as Europe, South America, China, and Japan. To date, several species of bromophenol compounds have been reported from Morrow red thread, and physiological activities include α-glucosidase inhibitory activity [Fisheries Science (1999), 65 (2), 300-303] and fish pathogens. Antimicrobial activity against [J. Fish Pathol., 18 (2): 147-156 (2005)], a domestic patent relating to antiviral activity against fish disease virus [Food Sci. Biotechnol. 17 (5): 1074-1078 (2008); Korean Patent No. 10-0838621] and antioxidant activity [Food Sci. Biotechnol. 18 (1): 124-129 (2009). However, the ultraviolet absorbing effect of the Morrow red thread extract has not been reported so far.

The inventors of the present invention, while studying a functional pharmaceutical material using marine organisms, as well as the antioxidant effect according to the free radical scavenging activity in the cell caused by the extract of Morrow red thread, the effect of absorbing such ultraviolet rays itself and ultraviolet rays The present invention was completed by confirming that there is a cytoprotective effect through inhibition of apoptosis induced by irradiation.

Accordingly, an object of the present invention is to inhibit skin damage and skin damage caused by ultraviolet rays, including the extract of Morrow red thread having the effect of absorbing ultraviolet rays, free radical scavenging effect and inhibiting apoptosis induced by ultraviolet irradiation as an active ingredient. It is to provide a pharmaceutical composition for treatment.

It is another object of the present invention to provide a composition for absorbing ultraviolet rays comprising the extract of Morrow red thread as an active ingredient.

Still another object of the present invention is to provide a cosmetic composition for inhibiting skin damage and protecting skin by ultraviolet rays comprising the extract of Morrow red thread as an active ingredient.

In order to achieve the object of the present invention as described above, the present invention is a red thread ( Polysiphonia) morrowii Harvey) provides a pharmaceutical composition for inhibiting skin damage and treatment of skin by ultraviolet rays comprising the extract as an active ingredient.

In one embodiment of the present invention, the Morrow red thread extract may be water, an organic solvent or a mixed solvent extract thereof.

In one embodiment of the present invention, the Morrow red thread extract may be an ethanol extract.

In one embodiment of the present invention, the Morrow red thread extract has a skin protection effect through the mechanism of suppressing the free radicals induced by ultraviolet absorption, ultraviolet irradiation and inhibiting apoptosis induced by ultraviolet rays. It can have

In one embodiment of the present invention, the pharmaceutical composition is a composition in the form of an external preparation for skin selected from the group consisting of creams, gels, patches, sprays, ointments, warnings, lotions, linings, pasta and cataplasma. Can be.

In addition, the present invention is a red thread ( Polysiphonia) morrowii Harvey) provides a composition for absorbing ultraviolet rays comprising the extract as an active ingredient.

The present invention also provides a red thread ( Polysiphonia) morrowii Harvey) provides a cosmetic composition for inhibiting skin damage and protecting skin by ultraviolet rays comprising the extract as an active ingredient.

In one embodiment of the present invention, the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition It can be formulated as an essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, lipstick, makeup base, foundation, press powder, loose powder, eye shadow.

Morrow red thread extract of the present invention is not only excellent in the antioxidant effect through free radical scavenging activity in the cell generated by ultraviolet irradiation, but also directly absorbs the ultraviolet light itself and inhibits apoptosis induced by ultraviolet light. Since the effect can be excellent, the pharmaceutical composition of the present invention comprising this as an active ingredient can effectively protect skin cells from ultraviolet rays can be usefully used for skin protection and skin treatment. In particular, such a Morrow red thread extract is a natural substance, so it corresponds to an edible substance, the composition of the present invention comprising it as an active ingredient has a safe advantage even in long-term use.

1 is a measurement of the absorbance of the Morrow red yarn extract of the present invention in the ultraviolet region (250 ~ 400 nm) using a UV spectrophotometer.
Figure 2 by measuring the concentration of DPPH radical scavenging activity, H ₂ O ₂ treated cell reactive oxygen species scavenging activity, UV-B treated cell reactive oxygen species scavenging activity according to the concentration-specific treatment of the Morrow red thread extract of the present invention It is shown graphically (PME: Morrow red thread extract of the present invention, NAC: N-acetyl cysteine).
3 is a laser scanning microscope 5 PASCAL program (Carl Zeiss, Jena, Germany) for the generation of active oxygen species generated in the cell when UV-B irradiation after the treatment of the extract of the Morrow red thread of the present invention to the cells by concentration It is a photograph imaged using.
4 is a graph quantifying the fluorescence intensity according to the generation of reactive oxygen species in the cell shown in the image of FIG.
Figure 5 is a graph measuring the ESR signal using an ESR spectrometer to measure the scavenging activity of the peroxide anion according to the application of the Morrow red thread extract of the present invention (control: no treatment group).
Figure 6 is a graph measuring the ESR signal using an ESR spectrometer to measure the scavenging activity of hydroxyl radicals according to the application of the Morrow red thread extract of the present invention (control: no treatment group).
Figure 7 shows the graph by measuring the cell viability through MTT assay method after applying the Morrow red thread extract of the present invention to the cells by concentration.
Figure 8 shows the graph by measuring the cell viability through the MTT assay method when the UV-B irradiated after treating the extract of Morrow red thread of the present invention cells.
9 is a graph showing the SOD enzyme activity measured by UV-B irradiation after treatment with the Morrow red thread extract of the present invention (control: untreated group).
10 is a graph showing the measurement of the catalase enzyme activity when UV-B irradiation after the treatment of the Morrow red thread extract of the present invention (control: untreated group).
11 is a result of measuring the amount of protein expression of SOD and catalase in the cells by Western blotting when the extract of the Morrow red thread extract of the present invention to the cells by concentration.
FIG. 12A is a photograph photographed under a fluorescence microscope after staining a fragment of a nucleus of apoptotic bodies with Hoechst 33342, a DNA-specific fluorescent dye, when UV-B irradiation was performed on the cells of the Morrow red thread extract of the present invention (control: No treatment).
FIG. 12B is a graph quantifying the intracellular apoptotic bodies shown in the image of FIG. 12A compared to the control group (control: no treatment group).
13 is a graph showing the analysis of DNA fragments of cells according to the formation of apoptotic bodies when UV-B irradiated after treatment of the extract of the Morrow red thread of the present invention (control: untreated group).

The present invention is characterized in that it provides a composition for inhibiting skin damage and skin treatment (protection) by ultraviolet light comprising the extract of the marrow red thread as an active ingredient.

Morrow red thread belongs to Ceramiales (Rhodomelaceae) and is distributed in East-West-South coast including Jeju Island in Korea. Morrow red yarn fractions are known to have DPPH radical scavenging activity and have been reported for their functionality and biomass as a source of arachidonic acid and eicosapentaenoic acid.

The present invention by confirming that the Morrow red thread extract having the above characteristics can directly absorb ultraviolet rays as well as antioxidant effects through free radical scavenging activity, and has an excellent effect of inhibiting DNA damage induced by ultraviolet rays. It was first identified that it is effective for skin protection.

More specifically, in Example 2 below, the UV-B absorption ability of the Morrow red yarn extract of the present invention was confirmed as a high absorbance at 285nm corresponding to the UV-B range, through which the Morrow red thread extract of the present invention UV It was inferred that skin cells could be protected in response to -B irradiation.

In addition, in Example <3-1>, it was confirmed that when the DPPH radical scavenging activity was measured, it showed 51% scavenging activity at 100 μg / ml of the Morrow red thread extract of the present invention. As a result of measuring the active oxygen species in UV-B treated cells after application of the Morrow red thread extract of the present invention, it was confirmed that the positive control group was higher than the 26% indicated by the positive control group NAC at 30 μg / ml. In Example <3-3>, superoxide anion (O2-.) And hydroxyl radical (OH) scavenging activity in UV-B treated cells after application of the Morrow red thread extract of the present invention. As a result, all of them were significantly removed.

In addition, in Example 5 below, the effect of the Morrow red thread extract of the present invention on antioxidant enzymes (SDO and catalase) was investigated, and the extract of the present invention not only increases antioxidant enzyme activity but also increases antioxidant enzyme protein expression. I could confirm it.

In addition, in Example 6, it was confirmed that the Morrow red yarn extract of the present invention effectively inhibits DNA damage and apoptosis caused by UV-B irradiation.

That is, the composition of the invention having the characteristics as described above can be useful in inhibiting skin damage caused by ultraviolet rays and to protect the skin from UV rays, Morrow red thread (Polysiphonia morrowii Harvey) can be used as a pharmaceutical composition for inhibiting skin damage and skin treatment by ultraviolet rays containing the extract as an active ingredient, and the extract itself has an ultraviolet absorbing effect, Polysiphonia morrowii Harvey) can also be used as a composition for absorbing ultraviolet rays containing the extract as an active ingredient.

The Morrow red thread extract according to the present invention may be obtained by extraction and separation from nature using extraction and separation methods known in the art, and the 'extract' as defined in the present invention may be obtained by using an appropriate solvent. It is extracted from the red thread, and may include, for example, hot water extracts of Morrow red thread, polar solvent soluble extract or nonpolar solvent soluble extract.

As a suitable solvent for extracting the extract from the Morrow red thread, any solvent that is acceptable in the art may be used, and water or an organic solvent may be used. Examples of the solvent include alcohols having 1 to 4 carbon atoms, acetone, ether, and the like, including purified water, methanol, ethanol, propanol, isopropanol, butanol, Various solvents such as benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane may be used alone or in combination. But is not limited to.

As the extraction method, any one of the methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method and compression method can be selected and used. In addition, the desired extract may be further subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation to the method for producing the Morrow red thread extract of the present invention, any known method may be used.

For example, the Morrow red thread extract included in the composition of the present invention may be prepared in powder form by an additional process such as distillation under reduced pressure, freeze drying, or spray drying, which is extracted by the hydrothermal extraction or solvent extraction. Can be. Further, the primary extract can be further purified by using various chromatographies such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography and the like, You can get it.

Therefore, in the present invention, the Morrow red thread extract is a concept including all the extracts, fractions and purified products obtained in each step of extraction, fractionation or purification, their dilutions, concentrates or dried products.

According to a specific embodiment of the present invention, the preparation of the Morrow red thread extract of the present invention is carried out as follows.

The Morrow red thread extract of the present invention is the dried Morrow red thread collected in Jeju Island and then dried dried red thread in 80% ethanol three times at room temperature for 24 hours, filtered and concentrated under reduced pressure with a rotary concentrator to ethanol soluble components Can be obtained.

The composition of the present invention comprising such a Morrow red thread extract as an active ingredient may be a pharmaceutical composition.

The pharmaceutical composition of the present invention can be prepared by using pharmaceutically acceptable and physiologically acceptable adjuvants in addition to the above-mentioned active ingredients. Examples of the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, swelling agents, lubricants, A lubricant or a flavoring agent can be used.

The pharmaceutical composition may be formulated into a pharmaceutical composition containing at least one pharmaceutically acceptable carrier in addition to the above-described active ingredients for administration.

The pharmaceutical composition may be in the form of granules, powders, tablets, coated tablets, capsules, suppositories, liquids, syrups, juices, suspensions, emulsions, drops or injectable solutions. For example, for formulation into tablets or capsules, the active ingredient may be combined with an oral, non-toxic pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. Also, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included as a mixture. Suitable binders include, but are not limited to, natural and synthetic gums such as starch, gelatin, glucose or beta-lactose, natural sugars such as corn sweeteners, acacia, tracker candles or sodium oleate, sodium stearate, magnesium stearate, Benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like. Acceptable pharmaceutical carriers for compositions that are formulated into a liquid solution include sterile water and sterile water suitable for the living body such as saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, One or more of these components may be mixed and used. If necessary, other conventional additives such as an antioxidant, a buffer, and a bacteriostatic agent may be added. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to formulate into injectable solutions, pills, capsules, granules or tablets such as aqueous solutions, suspensions, emulsions and the like. Further, it can be suitably formulated according to each disease or ingredient, using the method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field.

In one embodiment of the present invention, the Morrow red thread extract of the present invention may be included in 0.00001 to 30% by weight based on the total weight of the composition.

In one embodiment of the present invention, the pharmaceutical composition of the present invention may be a topical skin composition. In addition, the composition of the present invention has a UV absorbing effect, it can also be used as a sunscreen or the like as a skin external preparation that can be applied to the skin or a cosmetic composition for UV absorption.

The dermatological pharmaceutical composition of the present invention is an external preparation for skin having an effect of protecting skin from ultraviolet rays, and it can be used as an external preparation for skin such as cream, gel, patch, spray, ointment, warning agent, lotion, liniment, pasta or cataplasma But it is not limited thereto.

In addition, the composition of the present invention may be a cosmetic composition for inhibiting skin damage and skin protection by ultraviolet rays comprising the extract of Morrow red thread as an active ingredient.

When the composition of the present invention is made of a cosmetic composition, the composition of the present invention may include not only the above-described Morrow red thread extract, but also components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, Conventional adjuvants such as vitamins, pigments and flavorings, and carriers.

In addition, the composition of the present invention may be used in addition to the above-mentioned Morrow red thread extract, in combination with the organic sunscreen that has been conventionally used as long as it does not impair the skin protection effect by reacting with the Morrow red thread extract.

Examples of the organic ultraviolet screening agent include glyceryl paraben, drometrizol trisiloxane, drometrizol, dicaloyl triolate, disodium phenyldibenzimidazole tetrasulfonate, diethylhexylbutamidotriazone, Hydroxybenzoylhexyl benzoate, di-methoxy cinnamate, a mixture of Rawson and dihydroxyacetone, methylenebis-benzotriazolyltetramethylbutylphenol, 4-methylbenzylidene camphor, menthyl anthranylate, benzophenone Benzophenone-4, benzophenone-8 (dioxyphenyl) benzene, butylmethoxydibenzoylmethane, bishexyloxyphenol methoxyphenyltriazine, synoxate, , Octocrylene, ethylhexylmethylmethylphenyl, ethylhexylmethoxy cinnamate, ethylhexylsalicylate, ethylhexyltriazone, isoamyl-p-methoxy cinnamate, polysilicon-15 (dimethicone, At least one selected from the group consisting of terephthalylidene dicam persulfonic acid and salts thereof, tiei-salicylate and aminobenzoic acid (parabe) can be used.

Examples of products to which the cosmetic composition of the present invention can be added include cosmetics such as astringent lotion, softening longevity lotion, nutrition lotion, various creams, essences, packs, foundation and the like, cleansing, cleanser, soap, .

As a specific formulation of the cosmetic composition of the present invention, there may be mentioned a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizing lotion, a nutrition lotion, a massage cream, It includes formulations such as soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, lipstick, makeup base, foundation, press powder, loose powder, eye shadow.

According to a preferred embodiment of the present invention, the content of the Morrow red thread extract of the present invention is 0.00001-30% by weight, preferably 0.5-20%, more preferably 1.0-10% by weight based on the total weight of the composition. . When the content of the Morrow red thread extract is less than 0.00001% by weight, the ultraviolet absorbing effect by the Morrow red thread extract is greatly reduced, and when it exceeds 30% by weight, skin irritation may occur, and formulation problems may occur. .

On the other hand, the cosmetic composition according to the present invention may be formulated by stabilizing the containment of the Morrow red thread extract inside the nanoliposomes. When the extract is contained inside the nanoliposomes, the components of the extract can be stabilized to solve problems such as precipitation formation, discoloration, and odor when formulated, and can increase the solubility and transdermal absorption of the components, thereby improving efficacy expected from the extract. Maximum expression can be achieved.

In the present invention, nanoliposome refers to a liposome having a typical liposome form and having an average particle diameter of 10 to 500 nm. According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposome is 50 to 300 nm. When the average particle diameter of the nanoliposome is more than 300 nm, improvement of skin penetration and improvement of formulation stability is very weak among technological effects to be achieved in the present invention.

The nanoliposomes used to stabilize the extract according to the present invention may be prepared by a mixture comprising a polyol, an oily component, a surfactant, a phospholipid, a fatty acid and water.

The polyol used in the nanoliposome of the present invention is not particularly limited and is preferably a polyol such as propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isopropylene glycol, pentylene glycol, erythritol, , Sorbitol, and mixtures thereof. The amount thereof is 10 to 80% by weight, preferably 30 to 70% by weight, based on the total weight of the nanoliposome.

The oil component used in the preparation of the nanoliposome of the present invention may be selected from a variety of oils known in the art and is preferably a hydrocarbon oil such as hexadecane and paraffin oil, Vegetable oils such as silicon oil such as dimethicone and cyclomethicone, sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil, ethoxylated alkyl ether oils, propoxylated alkyl ether oils ₄₀ is a fatty alcohol, and mixtures thereof -, phytosphingosine, sphingosine, and scan non-ping the same scan pinggo cannabinoid lipid, cholesterol side-by celebrity, sitosterol cholesteryl sulfate, cholesteryl sulfate, cytokines, C _10. The amount thereof may be 1.0 to 30.0% by weight, and preferably 3.0 to 20.0% by weight based on the total weight of the nanoliposome.

Any surfactant known in the art may be used as the surfactant used in the preparation of the nanoliposome of the present invention. For example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants can be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkyl acyl glutamates, alkyl phosphates, alkyl lactylates, dialkyl phosphates and trialkyl phosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkyl polyglycosides, polyglyceryl esters and sugar esters. Particularly preferred surfactants are polysorbates belonging to nonionic surfactants. The amount thereof may be 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight, based on the total weight of the nanoliposome.

The phospholipid, another component used in the preparation of the nanoliposomes of the present invention, is a lipophilic lipid that is used with both affinity lipids and is composed of natural phospholipids (e.g., egg yolk lecithin or soy lecithin, sphingomyelin) Dipalmitoyl phosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, unsaturated lecithin or saturated lecithin derived from natural origin extracted from soybean or egg yolk is preferable. Normally, the amount of phosphatidylcholine is 23 to 95% and the amount of phosphatidylethanolamine is 20% or less in natural derived lecithin. In the production of the nanoliposome of the present invention, the amount of the phospholipid to be used is 0.5 to 20.0% by weight, preferably 2.0 to 8.0% by weight based on the total weight of the nanoliposome.

Fatty acid to be used in nano-liposome preparation of the present invention is a higher fatty acid, preferably a C ₁₂ - ₂₂ as a saturated or unsaturated fatty acid of the alkyl chain, e.g., lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid . The amount thereof may be 0.05 to 3.0% by weight, preferably 0.1 to 1.0% by weight, based on the total weight of the nanoliposome.

The water used in the preparation of the nanoliposome of the present invention is generally deionized distilled water, and the amount thereof may be 5.0-40 wt% based on the total weight of the nanoliposome.

The preparation of nanoliposomes can be accomplished through a variety of methods known in the art, but most preferably is made by applying a mixture comprising the ingredients to a high pressure homogenizer. The preparation of a nanoliposome by a high pressure homogenizer can be carried out under various conditions (for example, pressure, number of times, etc.) depending on a desired particle size, and preferably at a high pressure homogenizer So that the nanoliposome can be produced.

The cosmetic composition for skin protection according to the present invention may contain 0.1 ~ 20.0% by weight, based on the nanoliposome filling weight, more preferably 1.0 ~ 10.0% by weight for the formulation of the Morrow red thread extract.

The composition of the present invention may also be a food composition, which may contain various flavors or natural carbohydrates as additional ingredients, as well as ordinary food compositions, in addition to the extract of Morrow red thread as an active ingredient. .

Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. The above-described flavors can be advantageously used as natural flavorings (tau martin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.).

The food composition of the present invention can be formulated in the same manner as the above pharmaceutical composition and used as a functional food or added to various foods. Foods to which the composition of the present invention can be added include, for example, beverages, meat, chocolates, foods, confectionery, pizza, ram noodles, other noodles, gums, candy, ice cream, alcoholic beverages, vitamin complexes, .

In addition, the food composition is a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, such as moor red thread extract as an active ingredient, coloring and neutralizing agents (cheese, chocolate, etc.), pectic acid and Salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks.

Morrow red thread extract as an active ingredient of the present invention is a natural substance, so there is almost no toxicity and side effects, so it can be used with confidence even for long-term use for the purpose of inhibiting skin damage by ultraviolet rays and protecting the skin for skin protection.

The health functional food of the present invention may be prepared and processed in the form of tablets, capsules, powders, granules, liquids, pills and the like for the purpose of inhibiting skin damage and protecting the skin by ultraviolet rays.

In the present invention, the term &quot; health functional food &quot; refers to foods manufactured and processed using raw materials or ingredients having useful functions in accordance with Law No. 6727 on Health Functional Foods. Or to obtain a beneficial effect in health use such as physiological action.

The health functional food of the present invention may include a conventional food additive, and the suitability as a food additive, unless otherwise specified, in accordance with the General Regulations of the Food Additives and General Test Methods approved by the Food and Drug Administration, etc. Judging by the standards and standards.

Examples of the items listed in the above-mentioned "food additives" include chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; Natural additives such as persimmon extract, licorice extract, crystalline cellulose, high color pigment and guar gum; L-glutamic acid sodium preparations, noodle-added alkalis, preservative preparations, tar coloring preparations and the like.

For example, the health functional food in the form of tablets is a mixture of excipients, binders, disintegrants and other additives with the extract of Morrow red thread, the active ingredient of the present invention by granulating in a conventional manner, then adding a lubricant and the like Compression molding or the mixture can be compression molded directly. In addition, the health functional food in the form of tablets may contain a mating agent and the like as necessary.

Hard capsules among the health functional foods in the form of capsules can be prepared by filling a mixture of a mixture of additives such as the excipient such as Morrow red thread extract of the present invention in a conventional hard capsule, soft capsule agent is a red capsule extract The mixture mixed with additives such as excipients can be prepared by filling in a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a coloring agent, a preservative and the like, if necessary.

The health functional food of the cyclic form may be prepared by molding a mixture of the Morrow red thread extract, the active ingredient of the present invention, an excipient, a binder, a disintegrant, and the like by a conventionally known method. It can be zeroed away or the surface can be coated with materials such as starch, talc.

The health functional food in the form of granules can be prepared in a granular mixture of a mixture of Morrow red yarn extract, an active ingredient of the present invention, an excipient, a binder, a disintegrant, and the like by a conventionally known method. It may contain an agent and the like.

The health functional food may be a beverage, a meat, a chocolate, a food, a confectionery, a pizza, a ramen, a noodle, a gum, a candy, an ice cream, an alcoholic beverage, a vitamin complex and a health supplement food.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  >

Statistical processing

The experimental results were expressed as mean ± standard deviation, and the results were analyzed by variance (ANOVA) using Tokey's test to analyze the difference. A p-value <0.05 was considered statistically significant.

reagent

1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, N-acetyl cysteine (NAC), 5,5-demethyl-1-pyrroline-N-oxide (DMPO), 2 ', 7'-dichlorodihydrofluorescein diacetate (DCF- DA), [3- (4,5-dimehtylthiazol-2-yl) -2,5-diphenyltetrazolium] bromide (MTT) and Hoechst 33342 dyes were purchased from Sigma chemical company (St. Louis, Mo, USA). Cu / Zn SOD and CAT antibodies were purchased from Biodesign International Company (Saco, Maine, USA). All other compounds and reagents used analytical grades.

< Example  1>

The Morrow Red Thread  Extract preparation

Morrow red yarn samples were collected from Jeju Island, and the specimens were deposited with the Jeju Biodiversity Research Institute (JBRI) and received JBRI-10365. The Morrow red thread extract of the present invention is the dried Morrow red thread collected in Jeju Island and then dried dried red thread in 80% ethanol three times at room temperature for 24 hours, filtered and concentrated under reduced pressure with a rotary concentrator to ethanol soluble components Could be obtained.

< Example  2>

The Morrow Red Thread  Extract UV -B Absorption capacity  Measure

In order to evaluate the ultraviolet absorbing ability of the Morrow red yarn extract of the present invention prepared in Example 1, the absorbance was measured in the ultraviolet region (250 ~ 400 nm) using a UV spectrophotometer (Shimadzu, UV-1800, Japan).

As a result, as shown in Figure 1, the peak appeared at 285nm corresponding to the UV-B range, it was confirmed that the high absorbance in this range. Therefore, through these results, the present inventors were determined that the Morrow red thread extract may act to absorb UV-B, thereby protecting cells in response to UV-B irradiation.

< Example  3>

The Morrow Red Thread  Extract Free radical Scatters  Measure

In order to confirm the free radical scavenging activity of the Morrow red thread extract of the present invention prepared through Example 1, DPPH radical scavenging activity, intracellular reactive oxygen species (ROS) scavenging activity, peroxide anion scavenging activity and hydroxyl radical scavenging activity Was measured.

<3-1> DPPH Radical  Measurement of scavenging activity

DPPH (1,1-diphenyl-2-picrylhydrazyl) analysis is a method of measuring the antioxidant capacity as an indicator of the degree to which the purple color is reduced by aromatic compounds and aromatic amines due to the electron donating ability of antioxidants. In this experiment, NAC (N-acetyl cysteine), which is well known for its antioxidant efficacy, was used as a positive control group.

First, the Morrow red thread extract of the present invention prepared in Example 1 was added to each solution at 6.25, 12.5, 25, 50, and 100 μg / ml by concentration to 1 × 10 ^-4 M DPPH solution in methanol, and then mixed vigorously. After 3 hours, the absorbance was measured at 520 nm using a spectrophotometer of the residual DPPH concentration.

As a result, as shown in Figure 2, it was found that the Morrow red thread extract of the present invention has a DPPH radical scavenging ability in a concentration-dependent manner, the extract of the present invention at 6.25μg / ml at 4%, 12.5μg / ml 6%, it was confirmed that the scavenging activity of 13% at 25μg / ml, 26% at 50μg / ml, 51% at 100μg / ml.

<3-2> intracellular Active oxygen species ( ROS ) Scavenging activity measurement

In order to check whether the Morrow red thread extract of the present invention has an activity of scavenging reactive oxygen species (ROS) generated in cells, the human keratinocytes (HaCaT cells) after treatment with the extract of the present invention, H ₂ O ₂ ROS levels in keratinocytes were measured by treatment or UV-B irradiation. In this case, free radical species was measured using DCF-DA (dichlorodihydrofluorescein diacetate). DCF-DA reacts with intracellular reactive oxygen species to produce a fluorescent substance (dichlorodihydrofluorescein (DCF)) and can measure intracellular reactive oxygen species by measuring fluorescence generated in the cell. In this experiment, NAC (N-acetyl cysteine), which is well known for its antioxidant efficacy, was used as a positive control group.

① Cell culture

Human keratinocytes (HaCaT cells) are 5% CO ₂ And in an incubator with at least 95% humidity at 37 ° C. Cultured in DMEM (Dulbeccos modified Eagle's medium) containing 10% heat-inactivated fetal calf serum, 100μg / ml streptomycin and 100 units / ml penicillin.

② H ₂ O ₂  In treated cells Active oxygen species  Measure

The cultured human keratinocytes (HaCaT cells) were plated at a density of 1.5 × 10 ⁵ cells / well on a plate and incubated for 16 hours, followed by concentration of Morrow red thread extract of the present invention (6.25, 12.5, 25, 50, 100 μg / ml), and after 30 minutes H ₂ O ₂ (1 mM) was added to the plate and further incubated at 37 ° C. for 30 minutes. Finally, 10 minutes after the addition of 25 μM of DCF-DA solution, fluorescence of 2 ', 7'-dichlorofluorescein was detected using a PerkinElmer LS-5B spectrofluorometer (PerkinElmer, Waltham, MA, USA).

As a result, as shown in Figure 2, the Morrow red thread extract of the present invention was confirmed to erase the ROS in H ₂ O ₂ -induced cells in a concentration-dependent manner, the extract of the present invention was treated with 6.25μg / ml In this case, ROS scavenging activity was 14%, 17% when treated with 12.5μg / ml, 25% when treated with 25μg / ml, 47% when treated with 50μg / ml, and 100μg / ml. One case showed 61% ROS scavenging activity.

③ UV In B-treated cells Active oxygen species  Measure erasure effect

In this experiment, in order to confirm the effect of scavenging the active oxygen species in the cells generated by UV-B irradiation, we investigated the effect of scavenging the active oxygen species after irradiating UV-B to human keratinocytes (HaCaT cells).

First, the human keratinocytes (HaCaT cells) are plated at a density of 1.5 × 10 ⁵ cells / well on a plate and incubated for 16 hours, and then the Morrow red thread extract of the present invention is concentrated by concentration (6.25, 12.5, 25, 50, 100 μg / ml). After 1 hour, the cells were irradiated with UV-B (280-320 nm; peak intensity 302 nm) at 150 mJ / cm ₂ using a CL-1000M UV Crosslinker (UVP, Upland, CA, USA). The cells were incubated for 24 hours at 37 ℃. Finally, 10 minutes after the addition of 25μM of DCF-DA solution, the fluorescence of 2 ', 7'-dichlorofluorescein was detected using a PerkinElmer LS-5B spectrophotometer (PerkinElmer, Waltham, MA, USA).

As a result, as shown in Figure 2, it was confirmed that the Morrow red thread extract of the present invention has the activity of eliminating intracellular ROS generated by UVB in a concentration-dependent manner, the extract of the present invention is 14 at 6.25μg / ml %, 15% at 12.5μg / ml, 16% at 25μg / ml, 24% at 50μg / ml, it was confirmed that the ROS scavenging activity of 30% at 100μg / ml.

In addition, 2 × 10 ⁵ cells / well was dispensed on a cover-slip loaded 6-well plate and cultured for 16 hours for image analysis according to the generation of reactive oxygen species in the cell. , 50, 100 μg / ml). One hour after the extract was treated, the plate was irradiated with UV-B for 82 hours. And after 24 hours, DCF-DA (100 μM) was added to each well and the cells were incubated for additional 30 minutes at 37 ° C. After washing the cells with PBS, the stained cells were imaged using a laser scanning microscope 5 PASCAL program (Carl Zeiss, Jena, Germany) on a confocal microscope.

As a result, as shown in Figure 3, the red fluorescence intensity was matched with the DCF produced by ROS, when the fluorescence intensity was compared with the control group treated with 25μg / ml Morrow red thread extract of the present invention In the experimental group, it was confirmed that the fluorescence intensity value of 41 was 50 μg / ml, and the fluorescence intensity value of 25 appeared in the experimental group treated with 50 μg / ml, and the fluorescence intensity value of 20 appeared in the experimental group treated with 100 μg / ml. This result reflects the reduction of ROS generation as the Morrow red thread extract treatment of the present invention reduces the ROS signal according to UV-B irradiation (see FIG. 4).

<3-3> Peroxide Anion ( superoxide anion : O2 -·) Scavenging activity measurement

Peroxide anion produced through xanthine / xanthine oxidase system forms DMPO / .OOH adduct by DMPO (5,5 dimethyl-1-pyrroline-N-oxide), a spin trapping agent, and then the amount of DMPO / .OOH adduct is increased. It was measured using an electron spin resonance (ESR) spectrometer. Briefly, 20 μl of xanthine oxidase (0.25 U / mL) was added to 20 μl of xanthine (5 mM), 20 μl of DMPO (1.5 M) and 20 μl of Morrow Red Thread Extract (100 μg / mL), and the ESR signal was recorded after 5 minutes. . The conditions of the ESR spectrum are shown in Table 1 below.

parameter Condition magnetic field 336mT power 1.00 mW frequency 9.4380 GHz modulation amplitude 0.2mT gain 500 scan time 0.5 min scan width 10 mT time constant 0.03sec 온도 25

As a result, as shown in FIG. 5, the ESR spectrometer data showed that the peroxide anion signal increased to a value of 2756 in the xanthine / xanthine oxidase system, but the peroxide anion signal was treated when the Morrow red thread extract of the present invention was treated. Reduced to a value of 1552. It was confirmed to be a signal formed by removing the inhibition and ^- This enables the extract of the present invention O2.

<3-4> hydroxyl Radical ( hydroxyl radical : ㆍ OH ) Scavenging activity measurement

The hydroxyl radical generated by the Fenton reaction (H ₂ O ₂ + FeSO ₄ ) forms DMPO / .OH adduct by DMPO (5,5 dimethyl-1-pyrroline-N-oxide), a spin trapping agent, and then DMPO. OH adduct amount was measured using a JES-FA ESR spectrometer (JEOL, Tokyo, Japan). 0.3 M of DMPO, 10 mM FeSO 4, 10 mM H ₂ O ₂ ESR signal was recorded 2.5 minutes after mixing 100 μg / ml Morrow red thread extract with a phosphate buffer solution of pH 7.4. The conditions of the ESR spectrum are shown in Table 2 below.

parameter Condition magnetic field 336mT power 1.00 mW frequency 9.4380 GHz modulation amplitude 0.2mT gain 200 scan time 0.5 min scan width 10 mT time constant 0.03sec 온도 25

As a result, as shown in FIG. 6, ESR spectrometer data showed that the hydroxyl radical signal was increased to the value of 3930 under the Fenton reaction system, and the hydroxyl radical signal was reduced to the value of 2182 when the Morrow red thread extract of the present invention was treated. Appeared to be. Through this, the extract of the present invention was confirmed that the signal formation is inhibited by removing the OH.

< Example  4>

The Morrow Red Thread  Effect of Extracts on Cells

In this experiment, the cell survival rate was investigated by using MTT assay to observe the effect of Morrow red thread extract of the present invention on cell growth.

<4-1> Cytotoxicity measurement by concentration

After dispensing human keratinocytes (HaCaT cells) in 96 well plates at 1 × 10 ⁵ cells / ml and incubating for 16 hours in a 37 ° C., 5% CO ₂ incubator, the Morrow red thread extract of the present invention was 6.25, 12.5, It was added at concentrations of 25, 50 and 100 μg / ml, respectively. For MTT assay, 50μL of MTT stock solution (2mg / mL) was added to each well so that the total reaction volume was 200μL, and the cells were further cultured for 4 hours to reduce MTT. The plate was centrifuged at 800 × g for 5 minutes, the supernatant was removed, 150 μl dimethyl sulfoxide (dimethylsulfoxide) was added to each well to dissolve the formazan crystal, and the absorbance was measured at 540 nm with a scanning multi-well spectrophotometer. This absorbance represents the amount of MTT reduced by the cells and is therefore proportional to the cell viability present in each well.

As a result, as shown in Figure 7, the extract of the present invention did not appear cytotoxic at any concentration of 6.25, 12.5, 25, 50, 100μg / ml. Based on these results, it was determined that the extract of Morrow red thread of the present invention is an optimal amount of 100 μg / ml having high antioxidant effect, and in the following, the concentration of the Morrow red thread extract of the present invention was 100 μg / ml. .

<4-2> After the extract treatment of the present invention UV -Cell survival rate when -B irradiation

In this experiment, the effect of UV-B irradiation on the cells and the effect of protecting the cells in response to UV-B irradiation when treated with the Morrow red thread extract of the present invention.

Human keratinocytes (HaCaT cells) were dispensed in 96 well plates at 1 × 10 ⁵ cells / ml and incubated in a 37 ° C., 5% CO ₂ incubator for 16 hours, followed by 100 μg / ml of the Morrow red thread extract of the present invention. Added. After 1 hour, UV-B was irradiated and incubated for 48 hours at 37 ° C. For MTT assay, 50 μL of MTT stock solution (2 mg / mL) was added to each well so that the total reaction volume was 200 μL. Cells were further incubated for 4 hours to allow MTT to be reduced. The plate was centrifuged at 800 × g for 5 minutes, the supernatant was removed, 150 μl dimethyl sulfoxide (dimethylsulfoxide) was added to each well to dissolve the formazan crystal, and the absorbance was measured at 540 nm with a scanning multi-well spectrophotometer. This absorbance represents the amount of MTT reduced by the cells and is therefore proportional to the cell viability present in each well. PBS was used instead of the extract of the present invention as a control.

As a result, as shown in Figure 8, in the experimental group irradiated with UV-B, the cell survival rate was 79% in the experimental group irradiated with UV-B after treatment with the Morrow red thread extract of the present invention compared with 28% cell viability in the experimental group. It was confirmed that the increase significantly.

< Example  5>

The Morrow Red Thread  Effect of Extracts on Antioxidant Enzymes

In Example 3, it was confirmed that the Morrow red thread extract of the present invention shows a free radical scavenging effect, in the present experiment to check whether the free radical scavenging ability is affected by the antioxidant enzyme as the Morrow red thread extract of the present invention Antioxidase (SDO and catalase) activity and protein expression levels in treated cells were investigated.

<5-1> SOD ( superoxide dismutase ) Activity measurement

Superoxide dismutase (superoxide dismutase: SOD) is a representative radical anion as peroxide that can be in the human body: ^- an antioxidant for changing the (superoxide anion and O2), hydrogen peroxide (H ₂ O _2).

Human keratinocytes (HaCaT cells) were treated with Morrow's red thread extract of the present invention at a concentration of 100 μg / ml for 24 hours. After 1 hour, the cells were irradiated with UV-B and incubated at 37 ° C. for 24 hours in an incubator. SOD activity was measured according to the manufacturer's protocol using a colorimetric assay kit (Abcam, Cambridge, MA, USA). The kit used tetrazolium salt WST-1, which can produce water-soluble formazan as the peroxide anion decreases, and the formazan dye product was detected at 450 nm. SOD activity of peroxide anion ^- are shown as percent inhibition of (superoxide anion and O2).

As a result, as shown in Figure 9, the intracellular SOD activity was reduced to 54 mU / ml by UV-B irradiation, but the enzyme activity is restored to 61 mU / ml in the experimental group treated with the Morrow red thread extract of the present invention I could confirm that.

<5-2> Catalase ( catalase ) Activity measurement

Catalase is an enzyme that converts hydrogen peroxide into water. It is an antioxidant enzyme that primarily protects lipid peroxidation and inhibits radical production. Therefore, the present inventors confirmed whether the Morrow red expression extract of the present invention has antioxidant activity by regulating the activity of catalase, a radical production inhibitory enzyme, through the following experiment.

Morrow red yarn extract was treated with human keratinocytes (HaCaT cells) at 100 μg / ml for 24 hours, and after 1 hour, the cells were irradiated with UV-B and incubated at 37 ° C. for 24 hours in an incubator. Catalase activity was measured according to the manufacturer's protocol using a colorimetric assay kit (Abcam, Cambridge, MA, USA). Briefly, catalase reacts with methanol, which serves as an electron donor in the presence of H ₂ O ₂ . Formaldehyde produced was measured using 4-amino-3-hydrazino-5-mercapto-1,2,4-trizole as chromogen and detected at 540 nm. Catalase activity is expressed as nmol / min / ml.

As a result, as shown in FIG. 10, the intracellular CAT activity was reduced to 10 nmol / min / ml by UV-B irradiation, but in the experimental group treated with the Morrow red thread extract of the present invention to 21 nmol / min / ml. It was confirmed that the enzyme activity was restored.

<5-3> intracellular SOD  And Catalase  Protein expression level measurement

In order to investigate whether the Morrow red thread extract of the present invention affects the activity of SOD and catalase in cells as well as their protein expression, protein expression levels of SOD and catalase were measured by western blotting.

Human keratinocytes (HaCaT cells) were treated with Morrow red thread extract at 100 μg / ml concentration for 1, 6, 12, 24 hours, and then obtained by dissolving buffer (120 mM NaCl, 40 mM Tris (pH 8) and 0.1% NP 40). Treated with 100 μl for 30 minutes. Cells were then centrifuged at 13,000 × g for 15 minutes, then the supernatant was separated from the cell eluate, and protein concentration was determined. Some of the cell eluate (40 μg) was then heated for 5 minutes followed by electrophoresis on a 10% SDS-polyacrylamide gel and transferred to nitrocellulose membrane. 10% skim milk was then pretreated on the nitrocellulose membrane, and the secondary immunoglobulin-G-horseradish peroxidase conjugate (Pierce, Rockford, IL, USA) after treatment with the primary antibody against the protein of interest. Was treated. Protein bands were detected using an enhanced chemiluminescence western blotting detection kit (Amersham, Little Chalfont, Buckinghamshire, UK).

As a result, as shown in Figure 11, the experimental group treated with the Morrow red thread extract was confirmed that the time-dependent increase in the amount of Cu / Zn SOD and catalase protein expression compared to the control (β-actin).

< Example  6>

Morrow Red Thread  Extract UV Natural death by -B apoptosis Inhibitory Effect Analysis

In Example <4-2>, it was confirmed that cell death by UV-B was suppressed by the Morrow red thread extract treatment of the present invention. In order to investigate whether apoptosis by UV-B is directly related to apoptosis, the present inventors measured apoptosis inhibitory effect according to the Morrow red thread extract treatment of the present invention.

For apoptosis, apoptotic bodies were first observed through nuclear staining of cells. Human keratinocytes (HaCaT cells) were treated with Morrow red thread extract at a concentration of 100 μg / ml, and cells were exposed to UV-B after 1 hour. Cells were additionally incubated for 48 hours at 37 ° C., and 1.5 μl of Hoechst 33342 (stock 10 mg / ml), a DNA-specific fluorescent dye, was added to each well and incubated at 37 ° C. for 10 minutes. Stained cells were visualized and quantified under a fluorescence microscope equipped with a CoolSNAP-Pro color digital camera to examine the degree of nuclear condensation.

As a result, as shown in Figure 12, in the experimental group irradiated with UV-B, the fragments of the nucleus of the apoptotic bodies showing the natural apoptosis were remarkably observed, whereas the experimental group irradiated with UV-B after treatment with the Morrow red thread extract of the present invention In Esau, nuclear fragments of apoptotic bodies were rapidly reduced.

In addition, the DNA fragmentation of the cells was observed in order to measure the cytotoxic effect of the Morrow red thread extract of the present invention. DNA fragmentation of cells was assessed as cytoplasmic histone-related DNA fragments analyzed using kits purchased from Roche Diagnostic (Portland, OR, USA) according to the manufacturer's instructions.

As a result, as shown in Fig. 13, DNA fragmentation was high in UV-B irradiated cells, while DNA-fragmentation was significantly reduced in the experimental group irradiated with UV-B after treatment with the Morrow red thread extract of the present invention. .

Therefore, the present inventors have found that the Morrow red thread extract effectively inhibits the inhibition of apoptosis by UV-B irradiation through the degree of nuclear condensation and DNA fragmentation experiments. It was found that the silk extract was excellent in protecting the cells from UV-B irradiation.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (5)

Morrow Red Thread ( Polysiphonia) morrowii Harvey) A pharmaceutical composition for protecting skin cells against ultraviolet rays comprising the extract as an active ingredient.
The method according to claim 1,
Morrow red thread extract is a pharmaceutical composition for protecting skin cells against ultraviolet rays, characterized in that water, organic solvents or mixed solvent extracts thereof.
3. The method of claim 2,
The Morrow red thread extract is a pharmaceutical composition for protecting skin cells against ultraviolet light, characterized in that the ethanol extract.
The method according to claim 1,
The Morrow red thread extract has a medicament for protecting skin cells against ultraviolet rays, characterized in that it has the effect of eliminating free radicals in cells generated by ultraviolet absorption, ultraviolet irradiation, and inhibiting apoptosis induced by ultraviolet rays. Pharmaceutical composition.
The method according to claim 1,
The pharmaceutical composition is a composition for external application to the skin, characterized in that the composition for the skin, selected from the group consisting of creams, gels, patches, sprays, ointments, warnings, lotions, linings, pasta and cataplasma. Pharmaceutical composition for cell protection.

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