KR20130121385A - A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same - Google Patents

A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same Download PDF

Info

Publication number
KR20130121385A
KR20130121385A KR1020120044536A KR20120044536A KR20130121385A KR 20130121385 A KR20130121385 A KR 20130121385A KR 1020120044536 A KR1020120044536 A KR 1020120044536A KR 20120044536 A KR20120044536 A KR 20120044536A KR 20130121385 A KR20130121385 A KR 20130121385A
Authority
KR
South Korea
Prior art keywords
weight
granules
blueberry
present
audi
Prior art date
Application number
KR1020120044536A
Other languages
Korean (ko)
Inventor
최향자
홍주헌
이대훈
양수진
강은지
박혜미
Original Assignee
주식회사 소리소
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 주식회사 소리소 filed Critical 주식회사 소리소
Priority to KR1020120044536A priority Critical patent/KR20130121385A/en
Publication of KR20130121385A publication Critical patent/KR20130121385A/en

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/1682—Processes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/45—Ericaceae or Vacciniaceae (Heath or Blueberry family), e.g. blueberry, cranberry or bilberry
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
    • A61K36/605—Morus (mulberry)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/79—Schisandraceae (Schisandra family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention provides granules with improved storage stability of anthocyanin pigments of mulberry, blueberry, and Schisandra chinensis extracts which have physiological activities such as anticancer, anti-diabetes, anti-inflammation, anti-hyperlipidemia, and antioxidation; and a health food material and a health food using the same. [Reference numerals] (AA) 2L extraction solvent/100g extraction specimen;(BB) ��xtraction specimen: dried specimen of mulberry, blueberry, or schisandra chinensis;(CC) ��xtraction solvent: 1% Citric acid, 0.5% HCI, 50% EtOH, 48.5 % H_2O;(DD) Stir and extract at 160rpm and at room temperature;(EE) 48 hours;(FF) Distillation; remove impurities;(GG) Filtrate;(HH) Concentrate at reduced pressure;(II) ��oncentration standard: 30 Brix;(JJ) Common granule production;(KK) Decompressed concentration (30 brix) 20%, anhydrous purified glucose 25%, salty water crystal 45%, lactose powder 4.97%, maltodextrin 5%, flavor (Ugawa flavor) 0.03%;(LL) Present invention's granule production;(MM) Decompressed concentration (30 brix) 20%, anhydrous purified glucose 25%, salty water crystal 45%, lactose powder 4.97%, maltodextrin 5%, flavor (Ugawa flavor) 0.03%, alginic acid sodium salt 2%, liquid calcium 1.5%

Description

오디·블루베리·오미자 추출물의 안토시아닌 색소의 저장 안정성이 향상된 과립체 및 그 제조방법{A granule improved stock stability of anthocyanin pigment from Mulberry, Blueberry and S. chinensis Baillon extracts and manufacturing method of the same} Granule improved stock stability of anthocyanin pigment from Mulberry, Blueberry and S. chinensis Baillon extracts and manufacturing method of the same}

본 발명은 항암, 항당뇨, 항염증, 항고지혈증, 항산화 등 생리활성을 갖는 오디, 블루베리, 오미자 추출물의 안토시아닌 색소의 저장 안정성이 증대된 과립체 및 그 제조방법에 관한 것이다.The present invention relates to granules having an increased storage stability of anthocyanin pigments of Audi, blueberries, and Schisandra chinensis extracts having physiological activities such as anti-cancer, anti-diabetic, anti-inflammatory, anti-hyperlipidemia, and antioxidants, and methods for producing the same.

인간을 포함한 생물은 호흡이라는 과정을 통하여 에너지를 얻고 신진대사를 하는 과정에서 활성산소(reactive oxygen species: ROS)를 생성한다. 활성산소는 불안정한 성격을 띠고 있어서 세포내 단백질이나 지질분자는 물론이고 유전정보를 함유한 DNA에 치명적인 손상을 입히는 것으로 알려져 있다. Organisms, including humans, obtain energy through metabolic processes and produce reactive oxygen species (ROS) during metabolism. Free radicals are known to be unstable and cause fatal damage to DNA containing genetic information as well as intracellular proteins and lipid molecules.

상기와 같은 활성산소는 질병 유발이나 생체방어기구에 이상이 초래되거나 각종 물질적, 화학적 요인들에 의하여 생성량이 증가 되고 생체내에 정상범위 이상으로 용량을 초과하게 될 경우 산화적 스트레스(oxidative stress)를 야기한다. 산화적 스트레스가 노화를 비롯하여 각종 질환(암, 동맥경화, 염증성 질환, 심혈관 질환 등)을 일으키는 중요한 원인임이 입증됨으로써 활성산소 소거활성을 갖는 항산화성 생체 기능 물질이 노화 억제 및 질환의 치료제로서 가능성이 크게 부각되고 있다.Such active oxygen causes oxidative stress when the disease is caused to cause disease or abnormality in the biological defense mechanism or when the amount of production is increased by various physical and chemical factors and the dose is exceeded in the body over the normal range. do. As oxidative stress has proven to be an important cause of aging and various diseases (cancer, arteriosclerosis, inflammatory diseases, cardiovascular diseases, etc.), antioxidant bio-functional substances with active oxygen scavenging activity are likely to be anti-aging and therapeutic agents for diseases. It is greatly highlighted.

현대인은 산소에 의한 산화적 스트레스(oxidative stress)에 항상 노출이 되어 있으며, 이러한 산화적 스트레스는 정상적인 경우 생체 내에 존재하는 항산화계에 의해 제거되지만 각종 환경 오염물질, 흡연, 알콜 및 방사선등에 의해서 인체에 산화적 스트레스를 가중시키고 있다. 인체는 산화촉진물질(prooxidant)과 산화억제물질(antioxidant)이 균형을 이루고 있으나 산화촉진 쪽으로 기울게 되면 산화적 스트레스가 유발되어 잠재적인 세포손상 및 병리적 질환을 일으키게 된다.Modern man is always exposed to oxidative stress caused by oxygen, and this oxidative stress is normally removed by the antioxidant system existing in the living body, but it is exposed to human body by various environmental pollutants, smoking, alcohol and radiation. It is increasing oxidative stress. The human body has a balance of prooxidants and antioxidants, but when inclined toward oxidation, oxidative stress is induced, causing potential cell damage and pathological diseases.

최근에 건강에 대한 관심이 증대됨에 따라 영양분 섭취와 질병 예방 차원에서 각종 과실 및 이를 이용한 여러 가지 가공품에 대한 소비 및 제품개발에 관한 연구가 활발이 진행되고 있다. 과실에는 강력한 항산화 효과를 가지는 여러 가지 생리활성 성분 즉, 비타민, 카로티노이드 및 플라보노이드와 같은 페놀 화합물이 많이 존재하는 것으로 알려지고 있어 질병 예방과 감소에 크게 기여한다고 보고되고 있다. Recently, as interest in health has increased, studies on consumption and product development of various fruits and various processed products using the same have been actively conducted in order to prevent nutrient intake and disease. It is reported that fruit contains many physiologically active ingredients, such as vitamins, carotenoids, and flavonoids, which have a strong antioxidant effect, and are reported to contribute greatly to disease prevention and reduction.

뽕나무 열매인 오디(Mulberry)는 한방에서 상심자로 불리며 강장제나 진정제로 사용된 예가 있고, 현대의학에서는 혈당강하작용에 대한 보고가 있다. 다량의 안토시아닌 색소를 함유하고 있으며 항당뇨, 항산화, 항염증 그리고 항고지혈증 등의 생리활성이 있어 기능성 식품의 소재로 알려져 있다. Mulberry fruit mulberry (Mulberry) is called as a heartache in oriental medicine and used as a tonic or sedative, and there are reports of hypoglycemic action in modern medicine. It contains a large amount of anthocyanin pigment and is known as a functional food material because of its physiological activities such as antidiabetic, antioxidant, anti-inflammatory and anti-hyperlipidemia.

블루베리(Blueberry)는 진달래 과(Ericaceae) 산 앵도나무 속(Vaccinium)에 속하는 관목성 식물로서 항산화 작용과 시력증진작용 등의 강력한 기능성을 보유한 원예작물의 하나로 병해충이 적어 무 농약으로 재배할 수 있는 친환경적 작물로 알려져 있다. Blueberry is a shrub plant belonging to the genus Vaccinium of the Ericaceae family, and it is a horticultural crop with powerful functions such as antioxidant activity and visual enhancement. It is known as an environmentally friendly crop.

오미자(Schisandra chinensis Baillon)는 중추억제 작용, 혈압 강하 작용 및 알콜 해독 작용이 있는 것으로 알려져 있는 생약재로서 암 예방 활성 노화 억제 면역조절작용 및 항균활성 등 다양한 생리적 기능성이 보고되고 있다.
Schisandra chinensis Baillon is a herb known to have central inhibitory effect, blood pressure lowering effect and alcohol detoxification effect, and various physiological functions such as cancer prevention activity, anti-aging immunomodulation activity and antibacterial activity have been reported.

상기 오디, 블루베리, 오미자에는 다량의 안토시아닌(anthocyanin) 색소가 다량 함유되어 있다. 안토시아닌은 수소 이온의 농도에 따라 빨간색, 보라색, 파란색 등을 띄며, 시력개선, 혈관질환 예방, 인슐린 생성증대, 항염, 기억력 개선 효과가 알려져 있으며, 그중 항산화 효과는 비타밀, 카로틴계 화합물, 셀레늄, 토코페놀 등 일반적으로 알려진 항산화 물질보다 뛰어난 것으로 보고되어 있다. The audi, blueberries, and Schizandra chinensis contain large amounts of anthocyanin pigments. Anthocyanins have red, purple, and blue colors depending on the concentration of hydrogen ions, and are known to improve eyesight, prevent vascular disease, increase insulin production, anti-inflammatory, and improve memory. Among them, antioxidant effects include vitamins such as vitamins, carotene compounds, selenium, It is reported to be superior to commonly known antioxidants such as tocophenols.

상기 안토시아닌과 같은 생리효능을 갖는 유효성분의 일반적인 섭취수단으로는 열수에 우리거나 타서 마시는 차의 형태가 널리 이용되고 있으나 근래에 들어서는 과립, 타블렛, 캅셀 등과 같은 섭취 편리성이 강조된 제품들이 생산되고 있다. 과립 제품은 분말제품보다 흐름성이 좋으며 물리적 화학적으로 안정하다. 그리고 미세한 분말의 경우 용해 시 용매 표면에 부유하여 용해성에 문제가 있으나 과립체으로 제조하면 이러한 문제점을 보완할 수 있다. 또한 제조 공정이 간단하고 자동충전기 개발로 대량 생산이 가능할 뿐만 아니라 휴대가 용이하여 건강식품에 많이 사용된다.
As a general intake means of the active ingredient having an physiological effect, such as anthocyanin, the form of tea or drinking in hot water is widely used in recent years, but products such as granules, tablets, capsules, etc. have been produced in recent years. . Granulated products have better flowability than powdered products and are physically and chemically stable. And in the case of fine powder is suspended on the surface of the solvent when dissolved, solubility problem, but if prepared in granules can compensate for this problem. In addition, the manufacturing process is simple and mass production is possible due to the development of automatic charger, and it is also used for health food because it is easy to carry.

일반적으로 안토시아닌과 같은 천연색소는 산소와의 결합이 용이하여 공기와 접촉하였을 때 변질이 쉬우며 색소 추출물은 저장방법 및 기간에 따라 안정성이 감소하여 안정성을 높이는 기술이 절실하지만 이와 같은 연구는 미흡한 실정이다. 식품 분야에서는 색소 추출물을 식품 첨가물 또는 기능성 소재로 사용하기 위해 상기 색소물질의 제제화 기술연구를 통하여 식품 소재의 산화방지 및 보존성 향상, 변화하기 쉬운 식품소재의 안정화, 불필요한 냄새의 차단, 액상식품의 고형화, 식품소재의 방출속도 조절, 제조공정의 개선 및 물성향상을 시도하고 있다.In general, natural pigments such as anthocyanins are easy to decompose when they come into contact with air because they are easily combined with oxygen, and pigment extracts are deteriorated in terms of storage stability and stability depending on the storage method and duration. However, such studies are insufficient. to be. In the field of food, the pigment extract is used as a food additive or functional material through research on the formulation technology of the pigment material to prevent oxidation and preservation of food materials, to stabilize food materials that are easy to change, to block unnecessary odors, and to solidify liquid foods. In addition, it is trying to control the release rate of food materials, improve the manufacturing process and improve the physical properties.

대한민국 공개특허 제10-2010-0058027호에서는 검정콩으로부터 안토시아닌 색소추출을 위한 추출용매에 구연산을 첨가하여 안토시아닌을 안정화하고 상기 추출한 안토시아닌을 진공포장 함으로서 그 저장성을 증대시키는 방법에 대해 개시하고 있으며, 대한민국 공개특허 제10-2009-0011603호에서는 오미자로부터 안토시아닌 고농도 추출기술과 상기 추출물로부터 안토시아닌을 컬럼크로마토그래피를 통해서 분리하는 기술에 대해서 개시하고 있다. 또 대한민국 등록특허 제10-0760263호 에서는 자미고구마로부터 광에 불안정한 안토시아닌색소를 광산화 방지제인 EDTA 또는 sodium ascorbate를 첨가하여 아토시아닌의 광안정성을 증진시키는 방법에 대해서 개시하였다. 이 밖에도 안토시아닌 색소의 안정성을 증진 시키는 방법은 대한민국 공개특허 제10-1989-0011960호, 대한민국 공개특허 제10-2011-0010011호, 대한민국 공개특허 제10-1987-0011205호에서 유기산 또는 HCl을 첨가하여 pH를 낮추거나 금속염을 첨가함으로써 색소의 안정화를 유도하거나, 단백질, 탄닌, 플라보노이드, 알카로이드 또는 아미노산 등을 안토시아닌에 결합시켜 안토시아닌 복합체를 형성하여 색소침착(copigmentation)하는 방법등에 대해서 개시하고 있다.Korean Unexamined Patent Publication No. 10-2010-0058027 discloses a method of increasing the shelf life by adding citric acid to an extraction solvent for extracting anthocyanin pigment from black soybean and stabilizing anthocyanin and vacuum-packing the extracted anthocyanin. Patent No. 10-2009-0011603 discloses a technique for extracting high concentrations of anthocyanins from Schisandra chinensis and a technique for separating anthocyanins from the extracts by column chromatography. In addition, the Republic of Korea Patent No. 10-0760263 discloses a method for enhancing the light stability of the athocyanin by adding EDTA or sodium ascorbate as an antioxidation agent to the anthocyanin pigment unstable to light from the sweet potato. In addition, the method of improving the stability of the anthocyanin pigment is disclosed in the Republic of Korea Patent Publication No. 10-1989-0011960, Republic of Korea Patent Publication No. 10-2011-0010011, Republic of Korea Patent Publication No. 10-1987-0011205 A method of inducing stabilization of a pigment by lowering the pH or adding a metal salt, or binding an protein, tannin, flavonoid, alkaloid, or amino acid to anthocyanin to form an anthocyanin complex, and a pigmentation (copigmentation).

이상에서 살펴본 바와 같이 상기 개시된 발명들은 안토시아닌 색소를 추출하는 과정이나 추출 후 보관하는 과정에 첨가물을 통하여 pH를 조절하거나, 산소를 차단하는 방법 등으로 안정화를 유도하였을 뿐, 제형 방법의 개선을 통하여 안토시아닌 색소의 저장 안정성를 증대한 기술은 개시된바 없다. As described above, the disclosed inventions only induce stabilization by adjusting the pH through additives or blocking oxygen in the process of extracting the anthocyanin pigment or in the process of storing after extraction, and improving anthocyanin by improving the formulation method. No technique has been disclosed that enhances the storage stability of the dye.

따라서 본 발명의 목적은, 항암, 항당뇨, 항염증, 항고지혈증, 항산화 등의 생리활성을 갖는 안토시아닌 색소가 다량 함유되어 있는 오디, 블루베리, 오미자 추출물의 저장안정성을 증대할 수 있는 과립체제조방법을 제공하는 데 있다. Therefore, an object of the present invention is to prepare a granule body which can increase the storage stability of the mulberry extract, blueberry, Schizandra chinensis extract containing a large amount of anthocyanin pigments having physiological activities such as anti-cancer, anti-diabetic, anti-inflammatory, anti-hyperlipidemia, antioxidant To provide a way.

본 발명의 다른 목적은, 오디, 블루베리, 오미자의 과립체을 통하여 고부가가치 가공식품 및 기능성 식품소재를 제공하는 데 있다.Another object of the present invention is to provide a high value-added processed foods and functional food materials through granules of Audi, blueberries, Schisandra chinensis.

본 발명의 상기 목적은 오디, 블루베리, 오미자 각각의 시료를 건조하는 단계와; 이와 별도로 추출용매를 제조하는 단계와; 상기 건조 오디, 블루베리, 오미자 각각 1 중량부에 대해 상기에서 제조한 추출용매 20 중량부를 첨가하여 안토시아닌이 함유된 추출물을 얻는 단계와; 상기에서 얻은 안토시아닌 함유 추출물의 불순물을 제거하는 단계와; 상기 여과물을 30 브릭스로 농축시키는 단계와; 안토시아닌 색소가 안정화된 과립체 제조를 위한 혼합물 100 중량%에 대해 상기에서 얻은 30 브릭스의 3종의 농축액을 각각 20중량%, 무수정제포도당 23.5 중량%, 함수결정 43 중량%, alginic acid sodium salt 2중량%, 액상칼슘 1.5중량%, 유당분말 4.97중량%, 말토덱스트린 5중량%, 향(우가와향) 0.03중량%의 비율로 혼합하여 3종의 혼합물을 얻는 단계와; 상기에서 얻은 3종의 혼합물을 각각 과립기를 통과시켜 3종의 과립체를 제조하는 단계와; 상기에서 얻은 본 발명 3종의 과립체 각각의 폴리페놀, 총 플라보노이드, 총 안토시아닌 함량을 측정하는 단계와; 상기에서 얻은 3종의 본 발명 과립체의 항산화 효과를 전자공여능, 자유전자 소거 능력, 산화물 소거능력 측정을 통하여 검정하는 단계와; 상기에서 얻은 본 발명 3종의 과립체 각각에 대한 형태학적 입자크기, 입자모양에 대해 측정하는 단계와; 본 발명 3종 각각의 과립체의 안토시아닌 변화 측정을 통하여 저장 안정성을 평가하는 단계를 통하여 달성하였다. The above object of the present invention comprises the steps of drying the samples of Audi, Blueberry, Schizandra; Separately preparing an extraction solvent; Obtaining an extract containing anthocyanin by adding 20 parts by weight of the extraction solvent prepared above with respect to 1 part by weight of each of the dried mulberry, blueberries and schisandra chinensis; Removing impurities of the anthocyanin-containing extract obtained above; Concentrating the filtrate to 30 brix; 20 wt% of the three concentrates of 30 Brix obtained above were 20 wt%, 23.5 wt% of unrefined glucose, 43 wt% of hydrous crystals, and alginic acid sodium salt 2 with respect to 100 wt% of the mixture for preparing anthocyanin pigment stabilized granules. Obtaining a mixture of three kinds by mixing at a ratio of weight%, liquid calcium 1.5 weight%, lactose powder 4.97 weight%, maltodextrin 5 weight%, and flavor (Ugawa flavor) 0.03 weight%; Preparing three granules through each of the three mixtures obtained above through a granulator; Measuring polyphenol, total flavonoids, and total anthocyanin content of each of the three granules of the present invention obtained above; Assaying the antioxidant effects of the three inventive granules obtained above by measuring electron donating ability, free electron scavenging ability and oxide scavenging ability; Measuring the morphological particle size and particle shape of each of the three granules of the present invention obtained above; Through the step of evaluating storage stability through measuring the anthocyanin change of each of the three granules of the present invention.

본 발명은, 항암, 항당뇨, 항염증, 항고지혈증, 항산화 등의 생리활성을 갖는 안토시아닌 색소가 다량 함유되어 있는 오디, 블루베리, 오미자 추출물에 함유된 안토시아닌 색소의 안정성을 증대할 수 있는 과립체 제조방법을 제공하는 효과가 있다. The present invention, granules that can increase the stability of the anthocyanin pigment contained in audi, blueberry, Schizandra chinensis extract containing a large amount of anthocyanin pigment having physiological activities such as anti-cancer, anti-diabetic, anti-inflammatory, anti-hyperlipidemia, antioxidant It is effective to provide a manufacturing method.

또, 본 발명은 오디, 블루베리, 오미자의 과립체를 통하여 고부가가치 가공식품 및 기능성 식품소재를 제공하는 효과가 있다.In addition, the present invention has the effect of providing a high value-added processed foods and functional food materials through the granules of Audi, blueberries, Schisandra chinensis.

도 1은 본 발명 안토시아닌 색소가 안정화된 오디·블루베리·오미자 과립체의 제조공정을 도식화한 것이다.
도 2는 본 발명 오디·블루베리·오미자 과립체의 농도 의존적 산화물 소거능력을 나타낸 결과이다.
도 3은 본 발명 오디, 블루베리, 오미자의 과립체를 gold ion coating 한 후 주사형 전자현미경(scanning electron microscope, 160A, Shimazu, Japan)을 이용하여 3.0kV에서 50배 및 100배 배율로서 본 발명 오디(A-1; 50배율, A-2 100 배율), 블루베리(B-1; 50배율, B-2 100 배율), 오미자(C-1; 50배율, C-2 100 배율) 각각의 입자 표면을 나타낸 그림이다.
도 4는 본 발명 오디, 블루베리, 오미자 각각의 과립체의 저장성을 평가하여 그 결과를 나타낸 그림이다(A; 통상의 과립체 제조방법으로 제조한 상기 3종의 과립체, B; 본 발명 과립체 제조방법으로 제조한 상기 3종의 과립체).BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic diagram illustrating a production process of an Audi, blueberry, and Schisandra granules in which the anthocyanin pigment of the present invention is stabilized.
Figure 2 is a result showing the concentration-dependent oxide scavenging ability of the present invention Audi blueberry Schizandra granules.
3 is a 50-fold and 100-fold magnification at 3.0 kV using a scanning electron microscope (scanning electron microscope, 160A, Shimazu, Japan) after gold ion coating the granules of the present invention Audi, blueberry, Schizandra chinensis Audi (A-1; 50x, A-2 100x), Blueberry (B-1; 50x, B-2100x), Schisandra (C-1; 50x, C-2 100x) Figure shows the particle surface.
Figure 4 is a figure showing the results of evaluating the shelf life of each of the granules of the present invention Audi, blueberry, Schisandra chinensis (A; the three granules prepared by a conventional granule manufacturing method, B; granules of the present invention) The three kinds of granules produced by a sieve production method).

본 발명은 안토시아닌 색소성분을 함유한 시료를 건조하는 단계와; 추출용매를 제조하는 단계와; 상기 건조시료 1중량%에 대해 상기에서 제조한 추출용매 20중량%를 첨가하여 안토시아닌 색소가 함유된 추출물을 얻는 단계와; 상기에서 얻은 안토시아닌 색소함유 추출물을 여과를 통하여 불순물을 제거하는 단계와; 상기 여과물을 30 브릭스로 농축시키는 단계와; 과립체 100중량%에 대해 상기 농축액 20중량%와 과립체 제조용 혼합물을 제조하는 단계와; 상기에서 얻은 혼합물을 과립기에 통과시켜 과립체를 제조하는 것을 특징으로 하는 안토시아닌 색소의 저장 안정성이 향상된 과립체를 제조하는 방법을 개시하였다. 그러나 본 발명 안토시아닌 색소의 저장안정성이 향상된 과립체 및 그 제조방법에 있어 안토시아닌 색소성분이 함유된 시료는 본 발명에 사용된 오디, 블루베리, 오미자에 한정되는 것은 아니다.The present invention comprises the steps of drying the sample containing an anthocyanin pigment component; Preparing an extractant; Obtaining an extract containing anthocyanin pigment by adding 20% by weight of the extraction solvent prepared above to 1% by weight of the dry sample; Removing impurities from the anthocyanin pigment-containing extract obtained by filtration; Concentrating the filtrate to 30 brix; Preparing a mixture for preparing the granules with 20% by weight of the concentrate relative to 100% by weight of the granules; Disclosed is a method for producing granules having improved storage stability of the anthocyanin pigment, characterized in that the granules are prepared by passing the mixture obtained above through a granulator. However, the samples containing anthocyanin pigment components in the granules and storage method of the storage stability of the anthocyanin pigment of the present invention is not limited to the mulberry, blueberry, Schizandra used in the present invention.

이하, 본 발명의 구체적인 내용을 바람직한 실시예 및 실험예를 통하여 상세히 설명하나, 하기 실시예 및 실험예에 의해 본 발명의 권리 범위가 한정되는 것은 아니다.Hereinafter, the specific contents of the present invention will be described in detail through preferred examples and experimental examples, but the scope of the present invention is not limited by the following examples and experimental examples.

<< 실시예Example 1> 본 발명 오디· 1> this invention Audi 블루베리Blueberries ·오미자 ·Schisandra 과립체의Granular 제조 Produce

1. 실험재료1. Experimental material

오디(선운산 농업 협동조합, Mulberry), 블루베리(햇님농원, Blueberry), 오미자(경북 문경시 동로면, Schisandra chinensis Baillon)는 모두 국내산으로 태영 엘·에스로부터 구입하여 본 발명에 사용하였다. 오디와 블루베리는 생물, 오미자는 건조된 것을 사용하여 -20℃이하의 암소에 보관하면서 추출용 시료로 사용하였다. Ody (Sununsan Agricultural Cooperative, Mulberry), Blueberry (Sunnyum Farm, Blueberry), Omija (Schisandra chinensis Baillon, Dongro-myeon, Myeonggyeong-si, Gyeongbuk) were all domestically used in the present invention. Audi and blueberries were used as biological samples, and Schizandra chinensis were dried and stored in the cow at -20 ℃ or lower.

2. 추출물의 제조2. Preparation of Extract

상기 준비한 오디, 블루베리, 오미자 각각의 시료 100 g(건조중량)을 상기 각각의 건조중량 1 중량%에 대해 20 중량%에 해당하는 추출용매(1% Citric acid 및 0.5% HCl을 함유하는 물과 50% 에탄올)에 첨가하여 실온에서 48시간 동안 160rpm으로 교반 추출하였다. 불순물을 제거하기 위해 Whatman No.2 여과지(Whatman International Ltd., England)를 이용하여 여과시켰다. 여과된 용액은 감압농축기(Model N-1N, Eyela Co., Tokyo, Japan)를 사용하여 30 Brix로 농축 시킨 뒤 대조구로 일반적인 과립체제조 방법인 농축액 20%에 무수정제포도당 25%, 함수결정 45%, 유당분말 4.97%, 말토덱스트린 5%, 향(우가와향) 0.03% 실험구와 수정된 방법인 농축액 20%에 무수정제포도당 23.5%, 함수결정 43%, alginic acid sodium salt 2%, 액상칼슘 1.5%, 유당분말 4.97%, 말토덱스트린 5%, 향(우가와향) 0.03% 비율로 혼합한 후 통상의 과립기를 통과시켜 제조하였다. 제조된 시료는 -70℃이하의 암소에 보관하면서 분석용 시료로 사용하였다.
100 g (dry weight) of each sample of the prepared Audi, blueberry and Schizandra chinensis extract (20% by weight of water containing 1% Citric acid and 0.5% HCl) with respect to 1% by weight of each dry weight and 50% ethanol) and extracted with stirring at 160 rpm for 48 hours at room temperature. Filtration was performed using Whatman No. 2 filter paper (Whatman International Ltd., England) to remove impurities. The filtered solution was concentrated to 30 Brix using a reduced pressure concentrator (Model N-1N, Eyela Co., Tokyo, Japan), and then, as a control, 20% of concentrated glucose, 25% of anhydrous glucose, water crystalline 45 %, Lactose powder 4.97%, maltodextrin 5%, fragrance (Ugawa fragrance) 0.03% In experimental and modified 20% concentrate, unrefined glucose 23.5%, hydrous 43%, alginic acid sodium salt 2%, liquid calcium 1.5%, lactose powder 4.97%, maltodextrin 5%, flavor (Ugawa fragrance) was mixed in a ratio of 0.03% and prepared by passing through a conventional granulator. The prepared samples were used as samples for analysis while kept in the dark below -70 ℃.

<< 실시예Example 2> 본 발명 오디· 2> present invention Audi 블루베리Blueberries ·오미자 ·Schisandra 과립체의Granular 성분 분석 Component analysis

1. 총 폴리페놀함량 측정 1 . Total Polyphenol Content Measurement

총 폴리페놀 함량은 Folin-Denis법에 따라 본 발명 오디, 블루베리, 오미자 각각의 추출액 1 mL에 1N Folin ciocalteu reagent 1 mL를 첨가하고 충분히 혼합한 다음 20% Na2CO3 1 mL를 첨가하고 실온의 암소에서 30분간 반응시킨 후 분광광도계(Ultraspec 2100pro, Amersham Co., Sweden)를 이용하여 725 nm에서 흡광도를 측정하였다. 이때 총 폴리페놀함량은 Tannic acid(Sigma Co., USA)를 정량하여 작성한 표준곡선으로부터 계산하였다.Total polyphenol content of Folin-Denis method invention mulberry, blueberry, Schizandra chinensis was added 1N Folin ciocalteu reagent 1 mL to 1 mL of each extract was thoroughly mixed and then 20% Na 2 CO 3 according to the After adding 1 mL and reacting for 30 minutes in the dark at room temperature, the absorbance was measured at 725 nm using a spectrophotometer (Ultraspec 2100pro, Amersham Co., Sweden). The total polyphenol content was calculated from the standard curve prepared by quantifying Tannic acid (Sigma Co., USA).

본 발명 오디, 블루베리, 오미자의 각각의 과립체 제조 후 측정한 총 폴리페놀함량은 하기 표 1에 정리하였다. 본 발명 오디, 블루베리, 오미자의 각각의 과립체의 총 폴리페놀함량은 상기 각각의 과립체 시료의 농도를 5 mg/mL로 보정한 후 동일한 조건에서 측정하였으며, 오디 과립체의 총 페놀 함량은 4.83 mg/mL로 가장 높게 나타났으며, 상기 오디 과립체의 총 페놀함량은 다른 2종의 과립체의 함량과 비료해서 2.4 내지 3.7배 높게 나타났다. 또 본 발명 블루베리 과립체의 총 폴리페놀함량은 2.58 mg/mL로 나타났으며, 본 발명 오미자 과립체는 1.23 mg/mL로 가장 낮게 나타났다.The total polyphenol content measured after the preparation of the granules of the present invention Audi, blueberry, Schizandra are summarized in Table 1 below. The total polyphenol content of each granule of the present invention Audi, blueberry, and Schizandra was measured under the same conditions after correcting the concentration of each granule sample to 5 mg / mL, the total phenolic content of the Audi granules The highest phenol content of the Audi granules was 2.4 to 3.7 times higher than that of the other two granules. In addition, the total polyphenol content of the blueberry granules of the present invention was found to be 2.58 mg / mL, and the schizandra granules of the present invention were the lowest as 1.23 mg / mL.

본 발명 오디·블루베리·오미자 과립체의 총 폴리페놀 함량Total Polyphenol Content of the Audi, Blueberry and Schisandra granules of the Invention SampleSample Total polyphenol content (mg/ml)Total polyphenol content (mg / ml) MulberryMulberry 4.830.016a 4.830.016 a BlueberryBlueberry 2.580.024b 2.580.024 b S.chinensis BaillonS.chinensis Baillon 1.230.063c 1.230.063 c

2. 총 플라보노이드함량 측정 2 . Total Flavonoid Content Measurement

총 플라보노이드함량 측정은 Davis법에 따라 수행하였으며, 본 발명 오디, 블루베리, 오미자 각각의 추출액 1 mL에 diethyl glycol 2 mL를 혼합하여 충분히 시료를 용해시킨 후 0.1N NaOH 200 μL와 혼합하여 37℃에서 1시간 반응시킨 후 분광광도계(Ultraspec 2100pro, Amersham Co., Sweden)를 이용하여 420 nm에서 흡광도를 측정하였다. 이때 총 플라보노이드 함량은 Rutin(Sigma, St, Louis, MO, USA)을 정량하여 작성한 표준곡선으로부터 계산하였다. The total flavonoid content was measured according to the Davis method, and 2 mL of diethyl glycol was mixed with 1 mL of the extract of each of the present invention of Audi, blueberry, and Schisandra chinensis. After reacting for 1 hour, the absorbance was measured at 420 nm using a spectrophotometer (Ultraspec 2100pro, Amersham Co., Sweden). Total flavonoid content was calculated from the standard curve prepared by quantifying Rutin (Sigma, St, Louis, MO, USA).

본 발명 오디, 블루베리, 오미자의 각각의 과립체 제조 후 총 플라보노이드 함량을 측정한 결과는 하기 표 2와 같다. 본 발명 오디, 블루베리, 오미자의 각각의 과립체의 총 플라보노이드 함량은 상기 각각의 과립체 시료의 농도를 10 mg/mL로 보정하여 총 플라보노이드 함량을 측정하였으며, 본 발명 오디, 블루베리, 오미자 과립체 각각의 총 플라보노이드 함량은 3.49, 1.45 및 0.94 mg/mL였다.  The results of measuring the total flavonoid content after the preparation of the granules of the present invention Audi, blueberry, Schizandra chinensis are shown in Table 2 below. The total flavonoid content of each granule of the present invention Audi, blueberry, Schizandra chinensis was measured by adjusting the concentration of each granule sample to 10 mg / mL, and the total flavonoid content was measured. The total flavonoid content of each sieve was 3.49, 1.45 and 0.94 mg / mL.

본 발명 오디·블루베리·오미자 과립체의 총 플라보노이드 함량Total Flavonoid Content of the Audi, Blueberry and Schisandra granules of the Invention SampleSample Total flavonoid content (mg/ml)Total flavonoid content (mg / ml) MulberryMulberry 3.490.097a 3.490.097 a BlueberryBlueberry 1.450.081b 1.450.081 b S.chinensis BaillonS.chinensis Baillon 0.940.037c 0.940.037 c

3. 총 안토시아닌함량 측정 3 . Total Anthocyanin Content Measurement

총 안토시아닌함량은 본 발명 오디, 블루베리, 오미자의 각각의 과립체의 건조분말 0.2 g을 dH2O 3 mL에 녹여 분석용 시료로 사용하였다. 상기 안토시아닌 분석용 3종의 각각의 시료 100 μL에 1900 μL의 pH 1.0 buffer(0.2M KCl + 0.2M HCl) 또는 pH 4.5 buffer(0.2M Potassium phosphate + 0.1M Citric acid)를 각각 혼합한 후 520 nm와 700 nm에서 흡광도를 측정하였다. 총 안토시아닌 함량 (mg/100g)은 cyanidin-3-glucoside의 몰흡광계수(ε=26,900 L·M-1·cm-1)를 이용하여 하기의 식에 의해 산출하였다. As for the total anthocyanin content, 0.2 g of the dry powder of each granule of the present invention Audi, blueberry, and Schizandra chinensis was dissolved in 3 mL of dH 2 O and used as a sample for analysis. 1900 μL of pH 1.0 buffer (0.2M KCl + 0.2M HCl) or pH 4.5 buffer (0.2M Potassium phosphate + 0.1M Citric acid) was mixed with 100 μL of each of the three anthocyanin assays, and 520 nm. Absorbance was measured at and 700 nm. The total anthocyanin content (mg / 100g) was calculated by the following formula using the molar extinction coefficient (ε = 26,900 L · M-1 · cm-1) of cyanidin-3-glucoside.

Figure pat00001
Figure pat00001

A : (Aλ520 - Aλ700)pH 1.0 - (Aλ520 - Aλ700)pH 4.5A: (A λ 520 -A λ 700 ) pH 1.0- (A λ 520 -A λ 700 ) pH 4.5

MW : cyanidin-3-glucoside의 분자량 ＝ 449.2 g/mol MW: Molecular weight of cyanidin-3-glucoside = 449.2 g / mol

DF : 희석배수 = 20 DF: Dilution factor = 20

3 : 총 부피 3 mL 3: total volume 3 mL

5000 : 시료 100 g당으로 환산하기 위하여 추출액의 시료 무게인 0.2 g으로 나눈 값 5000: The value divided by 0.2 g which is the sample weight of the extract to convert to 100 g of sample.

ε : 몰흡광계수 ＝ 26,900 L/cm·mol ε: molar extinction coefficient = 26,900 L / cmmol

1 : cm 1 cm

본 발명 오디, 블루베리, 오미자의 각각의 과립체의 총 안토시아닌 함량은 하기 표 3과 같다. 본 발명 오디, 블루베리, 오미자 각각의 과립체에는 안토시아닌이 다량 함유되어 있는데, 주요 안토시아닌으로는 오디에는 cyanidin-3-glucoside(C3G)와 cyanidin-3-rutinoside이 주로 함유되어 있으며, 그 비율은 7: 3으로 분포하고 있다. 또, 블루베리는 Delphinidin-3-arabinoside(D3A)의 함량이 가장 높으며, 오미자는 cyanidin-3-glucoside(C3G)와 Petunidin-3-glucoside(P3G)가 함유되어 있다. 안토시아닌 함량은 본 발명 오디 과립체에는 76.26 mg/100g , 본 발명 블루베리 과립체에는 75.26mg/100g으로 분포하며 상기 2종의 본 발명 과립체의 안토시아닌 함량은 크게 차이 나지 않았다. 그러나 본 발명 오미자 과립체의 안토시아닌 함량은 15.81mg/100g으로 함량이 가장 낮았다. The total anthocyanin content of each granule of the present invention Audi, blueberry, Schizandra chinensis is shown in Table 3 below. In the granules of the present invention Audi, blueberries, and Schizandra chinensis, anthocyanins are contained in a large amount. As main anthocyanins, Audi contains cyanidin-3-glucoside (C3G) and cyanidin-3-rutinoside, and the ratio is 7 : It is distributed as 3. Blueberries have the highest content of Delphinidin-3-arabinoside (D3A), and Schisandra chinensis contains cyanidin-3-glucoside (C3G) and Petunidin-3-glucoside (P3G). The anthocyanin content was distributed in 76.26 mg / 100g in the present invention granules, 75.26mg / 100g in the blueberry granules of the present invention, and the anthocyanin content of the two inventive granules was not significantly different. However, the anthocyanin content of the schizandra granules of the present invention was the lowest as 15.81mg / 100g.

본 발명 오디·블루베리·오미자 과립체의 안토시아닌 함량Anthocyanin Content of the Audi, Blueberry and Schisandra granules of the Invention SampleSample Total anthocyanin content (mg/100g)Total anthocyanin content (mg / 100g) MulberryMulberry 76.267.441a 76.267.441 a BlueberryBlueberry 75.2610.860a 75.2610.860 a S.chinensis BaillonS.chinensis Baillon 15.817.040b 15.817.040 b

<< 실시예Example 3> 본 발명 오디· 3> present invention Audi 블루베리Blueberries ·오미자 ·Schisandra 과립체의Granular 항산화 활성측정 Antioxidant Activity Measurement

1. 전자공여능 활성 측정 1 . Electron Donating Activity Measurement

본 발명 오디, 블루베리, 오미자 과립체 각각의 전자공여능 활성 측정은 1,1-diphenyl-2-pycrylhydrazyl(DPPH)의 환원력을 이용하여 측정하였다. DPPH 시약 12 mg을 Absolute ethanol 100 mL에 용해한 후 dH2O 100 mL 첨가하여 DPPH 용액의 흡광도를 517 nm에서 약 1.6으로 조정한 후, 본 발명 오디, 블루베리, 오미자 3종의 과립체 수용액 0.5 mL에 DPPH 용액 5 mL를 혼합하여 실온에서 15분간 방치 한 후 분광광도계(Ultraspec 2100pro, Amersham Co., Sweden)로 흡광도를 측정하여 하기와 같이 계산하여서 전자 공여능을 평가하였다.Electron-donating activity of each of the present invention Audi, blueberry, Schizandra granules was measured using the reducing power of 1,1-diphenyl-2-pycrylhydrazyl (DPPH). After dissolving 12 mg of DPPH reagent in 100 mL of Absolute ethanol and adding 100 mL of dH 2 O to adjust the absorbance of the DPPH solution to about 1.6 at 517 nm, 0.5 mL of aqueous solution of granules of the three species of Audi, blueberry and Schizandra chinensis of the present invention 5 mL of a DPPH solution was added to the mixture, and the mixture was left at room temperature for 15 minutes, and then absorbance was measured with a spectrophotometer (Ultraspec 2100pro, Amersham Co., Sweden).

Figure pat00002
Figure pat00002

전자 공여능은 활성산소에 전자를 공여하고 식품 중의 지방질 산화를 억제하는 목적으로 사용되며, 인체 내에서는 활성 라디칼에 의한 노화를 억제시키는 작용으로 이용되고 있다. 본 실험예에서는 본 발명 3종의 과립체 각각의 DPPH 라디칼 소거 활성능력을 평가하여 하기 표 4에 나타냈다. 상기 본 발명 3종의 과립체들의 농도는 20g/mL의 농도로 동일하게 보정한 후에 측정하였고 그 측정 값은 본 발명 오디 과립체가 45.09%로 가장 높은 소거 활성을 보였고, 본 발명 블루베리와 오미자 과립체 각각은 24.10% 및 15.80%로 나타났다. The electron donating ability is used for the purpose of donating electrons to active oxygen and inhibiting lipid oxidation in foods, and is used in the human body to suppress aging by active radicals. In this experimental example, the DPPH radical scavenging activity of each of the three granules of the present invention was evaluated and shown in Table 4 below. The concentration of the three granules of the present invention was measured after the same correction to the concentration of 20g / mL and the measurement value of the present invention the granules of the present invention showed the highest scavenging activity of 45.09%, the present invention blueberry and Schizandra granules The sieves were 24.10% and 15.80%, respectively.

본 발명 오디·블루베리·오미자 과립체의 전자공여능 Electron Donating Ability of the Audi, Blueberry and Schisandra granules of the Invention SampleSample Electron donating ability(%)Electron donating ability (%) MulberryMulberry 45.090.939a 45.090.939 a BlueberryBlueberry 24.100.154b 24.100.154 b S.chinensis BaillonS.chinensis Baillon 15.800.585c 15.800.585 c

2. ORAC ( Oxygen Radical Absorbance Capacity ) 측정 2 . ORAC ( Oxygen Radical Absorbance Capacity ) Measurement

본 발명 오디, 블루베리, 오미자 각각의 과립체 3종에 대한 항산화지수는 ORAC(Oxygen Radical Absorbance Capacity) 분석법을 통하여 측정하였다. 상기 ORAC측정법은 생체내의 수소전자 전달과 연관하여 AAPH(2,2'-azobis-2-methyl -propanimidamide, dihydrochloride)에 의해 생성된 자유전자 소거 능력, 즉 Radical chain breaking antioxidant capacity를 측정하는 것으로 측정하려는 시료에 존재하는 Hydrophobic 성분과 Hydrophlic 성분 모두와 반응하기 때문에 응용범위가 넓은 측정법으로 알려져 있다. 본 실험예에서는 본 발명 3종의 과립체 각각 및 표준액을 중성 Phosphate buffer(61.6:38.9 v/v, 0.75M K2HPO4 and 0.75M NaH2PO4)를 사용하여 농도별로 희석하였다. 검량 곡선을 작성하기 위하여 항산화 활성 비교 표준액으로 Trolox(Water soluble analogue of vitamin E, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, Aldrich Chem, Inc., USA) 10 μL를 Phosphate buffer 50 mL에 용해하여 제조하였고, 측정기기는 Fluorescent micro plate reader(Infinite M200 PRO, Tecan Co., Austria)를 사용하여 485 nm에서 정자가 여기(Excitation)되고 538 nm에서 방출(Emission)되게 조절하여 자유전자 소거능력을 측정하여 하기 표 5에 나타냈다. 표준시약으로 사용한 Trolox 함량과 형광도의 AUC 간의 회귀방정식은 Y=0.2818X+3.7577(Y는AUC이며, X는 Trolox 함량)이었다. Trolox의 함량이 증가함에 따라 형광도의 AUC도 유의하게(R2=0.944) 증가하였다. 본 발명 3종의 과립화 시료 각각은 1000 μg/mL 농도에서 실험을 수행하였다. 본 발명 오디 과립체의 항산화지수는 87.65μ moles TE /g FW로 측정되어 가장 높게 평가되었으며, 본 발명 블루베리 과립체의 항산화지수는 57.59 μ moles TE /g FW, 본 발명 오미자과립체의 측정결과는 47.72 μ moles TE /g FW로 나타났다. 이를 통해 본 발명 3종의 과립체중에 오디의 항산화능이 가장 높게 측정되었고, 본 발명 오미자 과립체가 가장 낮은 항산화지수를 나타냈다. Antioxidant index of each of three granules of Audi, blueberry and Schizandra chinensis was measured by ORAC (Oxygen Radical Absorbance Capacity) analysis. The ORAC assay is intended to measure free electron scavenging ability, ie, Radical chain breaking antioxidant capacity, generated by AAPH (2,2'-azobis-2-methyl-propanimidamide, dihydrochloride) in association with the delivery of hydrogen electrons in vivo. It is known to measure a wide range of applications because it reacts with both the hydrophobic and hydrophlic components present in the sample. In this experimental example, each of the three granules and the standard solution of the present invention were diluted by concentration using neutral Phosphate buffer (61.6: 38.9 v / v, 0.75M K2HPO4 and 0.75M NaH2PO4). To prepare the calibration curve, 10 μL of Trolox (Water soluble analogue of vitamin E, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, Aldrich Chem, Inc., USA) The solution was prepared by dissolving in 50 mL of phosphate buffer, and the measuring instrument was controlled to excitate at 485 nm and to emit at 538 nm using a fluorescent micro plate reader (Infinite M200 PRO, Tecan Co., Austria). It was shown in Table 5 to measure the free electron scavenging ability. The regression equation between Trolox content and AUC of fluorescence used as standard reagent was Y = 0.2818X + 3.7577 (Y is AUC and X is Trolox content). As the Trolox content increased, the AUC of fluorescence also increased significantly (R2 = 0.944). Each of the three granulation samples of the present invention was tested at a concentration of 1000 μg / mL. The antioxidant index of the present invention granules was measured by 87.65μ moles TE / g FW was the highest evaluation, the antioxidant index of the blueberry granules of the present invention was 57.59 μ moles TE / g FW, the measurement result of the present invention Schizandra granules Was 47.72 μmoles TE / g FW. Through this, the antioxidant activity of Audi was measured highest among the three granules of the present invention, and the present invention Schizandra granules showed the lowest antioxidant index.

본 발명 오디·블루베리·오미자 과립체의 자유전자 소거능력Free Electron Scavenging Ability of the Audi, Blueberry and Schisandra granules of the Invention SampleSample ORAC(moles TE/ g FW)ORAC (moles TE / g FW) MulberryMulberry 87.6512.753a 87.6512.753 a BlueberryBlueberry 57.596.013b 57.596.013 b S.chinensis BaillonS.chinensis Baillon 47.728.062b 47.728.062 b

3. Superoxide radical 소거 활성 측정 3 . Superoxide measurement of radical scavenging activity

Superoxide radical 소거 활성 측정은 Nishikimi et al의 방법에 의해 측정하였다. 본 발명 오디, 블루베리, 오미자 각각의 과립체의 최종반응농도가 100, 250, 500, 1000 μg/mL이 되도록 조제한 후 각각 0.5 mL씩 취해 0.1M Tris-HCl 완충용액(pH 8.5) 0.1 mL. 100μM PMS 0.2 mL을 혼합하여 반응시켰다. 상기 반응액은 560 nm에서 분광광도계(Ultraspec 2100pro, Amersham Co., Sweden)로 흡광도를 측정하여 이때의 흡광도를 S0으로 하고, 계속하여 500μM NBT 0.2 mL 및 500μM NADH 0.4 mL를 가한 후 2차 반응을 유도한 후에 그 반응물을 560 nm에서 흡광도를 측정하여 S로 하였고 이를 결과산출에 사용하였다. 또한 대조구로서 용매만을 사용하여 동일한 과정으로 측정하여 C0 및 C를 얻었고 산화물 소거능력을 하기와 같이 계산하였다. Superoxide radical scavenging activity was measured by Nishikimi et al. The final reaction concentrations of the granules of the present invention Audi, blueberry, Schizandra chinensis were prepared so that the final reaction concentration was 100, 250, 500, 1000 μg / mL, and 0.5 mL of each 0.1M Tris-HCl buffer (pH 8.5) 0.1 mL. 0.2 mL of 100 μM PMS was mixed and reacted. The reaction solution was measured by absorbance at 560 nm with a spectrophotometer (Ultraspec 2100pro, Amersham Co., Sweden) and the absorbance was set to S0. Subsequently, 0.2 mL of 500 μM NBT and 0.4 mL of 500 μM NADH were added, followed by secondary reaction. After induction, the reaction was measured as absorbance at 560 nm to be S, which was used for calculating the result. In addition, by using the solvent only as a control was measured in the same process to obtain C0 and C and the oxide scavenging ability was calculated as follows.

Figure pat00003
Figure pat00003

비효소적인 방법인 PMS/NADH로 유발된 Superoxide radical 생성계인 경우 NBT는 자주색의 Formazan으로 환원된다. 시료 내 Superoxide radical 소거 활성이 존재하는 경우, 시료첨가에 의해 Formazan의 생성이 억제되며 흡광도가 감소하게 된다. 추출물 무첨가군의 흡광도 변화를 100%로 기준하여 추출물 첨가군의 흡광도 변화를 Superoxide radical 생성 억제율로 표시하였다. In the non-enzymatic PMS / NADH-induced superoxide radical generation system, NBT is reduced to purple Formazan. If superoxide radical scavenging activity is present in the sample, formazan production is inhibited by absorbing the sample and the absorbance decreases. The absorbance change of the extract addition group was expressed as the inhibition rate of superoxide radical generation based on the change in absorbance of the extract-free group as 100%.

과립화된 본 발명 3종의 각각의 시료 농도에 대한 Superoxide radical 활성은 하기 표 6과 도 2에 나타내었다. 본 발명 오디 과립체 100 μg/mL 농도의 Superoxide 소거활성은 23.38%이었으며, 과립화된 시료의 농도가 증가할수록 소거활성도 증가하여 250, 500, 1000 μg/mL 농도에서 각 각 44.08%, 59.64%, 65.74%로 나타났다. 본 발명 블루베리 과립체 1000 μg/mL 농도 분석 결과는 65.74% 활성을 보여 오디 과립체과와 동일하게 나타났으며, 다른 농도인 100, 250, 500 μg/mL에서 블루베리 과립체의 활성이 각 각 16.03%, 39.313%, 56.87%로 나타나 본 발명 오디 과립체가 대체적으로 조금 높은 효과를 나타냈다. 본 발명 오미자 과립체는 1000 μg/mL에서 33.59%의 활성을 나타내어 본 발명의 다른 2종 과립체 오디 및 블루베리와 비교하여 50%정도의 활성만을 나타냈다. Superoxide radical activity of each of the three granulated sample concentrations of the present invention is shown in Table 6 and FIG. 2. Superoxide scavenging activity of 100 μg / mL concentration of the Audi granules of the present invention was 23.38%, and as the concentration of the granulated sample was increased, the scavenging activity was increased to be 44.08%, 59.64%, respectively at 250, 500, and 1000 μg / mL concentrations. 65.74%. The blueberry granules 1000 μg / mL concentration analysis result of the present invention showed 65.74% activity, the same as that of the Audi granules, the activity of the blueberry granules at different concentrations of 100, 250, 500 μg / mL 16.03%, 39.313%, 56.87%, the audi granules of the present invention showed a slightly higher effect. The schizandra granules of the present invention exhibited 33.59% of activity at 1000 μg / mL, showing only about 50% of activity compared to the other two granules of the present invention, audi and blueberries.

본 발명 오디·블루베리·오미자 과립체의 산화물 소거능력Oxide Scavenging Capacity of the Audi, Blueberry and Schisandra granules of the Invention SampleSample Superoxide radical scavenging activity (%)Superoxide radical scavenging activity (%) 100(g/mL)100 (g / mL) 250(g/mL)250 (g / mL) 500(g/mL)500 (g / mL) 1000(g/mL)1000 (g / mL) MulberryMulberry 23.381.863a 23.381.863 a 44.082.296b 44.082.296 b 59.640.496c 59.640.496 c 65.741.412d 65.741.412 d BlueberryBlueberry 16.033.061a 16.033.061 a 39.311.312b 39.311.312 b 56.873.625c 56.873.625 c 65.742.435d 65.742.435 d S.chinensis BaillonS.chinensis Baillon 9.062.296a 9.062.296 a 12.213.780a 12.213.780 a 26.535.792b 26.535.792 b 33.592.161b 33.592.161 b CatechinCatechin 40.741.594a 40.741.594 a 76.431.157b 76.431.157 b 89.031.005c 89.031.005 c 91.130.286d 91.130.286 d

<< 실시예Example 4> 본 발명 오디· 4> present invention Audi 블루베리Blueberries ·오미자 ·Schisandra 과립체의Granular 입자크기 및 모양측정 Particle Size and Shape Measurement

1. 입자크기 1 . Particle size

본 발명 3종의 과립체의 평균크기는 laser particle size analyzer(LS-320, Backman Coulter co., USA)를 이용하여 isopropyl alcohol에 분산시켜 측정하였다. 본 발명 오디, 블루베리, 오미자 각각 과립체의 Particle size 평균입도는 하기 표 7에 나타냈다. 본 발명 3종 각각의 과립체 분말의 입자크기는 306.4 ∼ 451.0 μm로 나타났고 포접액의 점도가 높을수록 입도가 커진다고 보고된 것으로 보아 블루베리의 점도가 가장 높았을 것으로 보여진다. 보통 미세캡슐화된 분말의 입자가 1∼200 μm의 범위에서 분포하는데 본 실험의 결과에서는 과립화된 입자로 크기가 미세분말보다 2배정도 크게 나타났고, 일반적으로 크기보다는 피막물질의 종류나 과립체 제조의 조건 등이 더 중요하다.  The average size of the three granules of the present invention was measured by dispersing in isopropyl alcohol using a laser particle size analyzer (LS-320, Backman Coulter Co., USA). Particle size average particle size of the present invention Audi, blueberry, Schizandra granules are shown in Table 7 below. The particle size of each of the three granular powders of the present invention was found to be 306.4 ~ 451.0 μm, and the higher the viscosity of the inclusion solution, the larger the particle size was reported. Usually, the particles of the microencapsulated powder are distributed in the range of 1 to 200 μm. In the results of this experiment, the size of the granulated particles was twice as large as that of the fine powder. The condition is more important.

본 발명 오디·블루베리·오미자 과립체의 입자크기Particle Size of the Audi, Blueberry and Schizandra Granules of the Present Invention SampleSample Particle size (μm)Particle size (μm) MulberryMulberry 306.4306.4 BlueberryBlueberry 451.0451.0 S.chinensis BaillonS.chinensis Baillon 443.0443.0

2. 입자모양 2 . Particle shape

본 발명 오디, 블루베리, 오미자 과립체 각각의 표면 구조는 gold ion coating한 후 주사형 전자현미경(scanning electron microscope, 160A, Shimazu, Japan)을 이용하여 관찰하였고, 현미경관찰 조건은 3.0kV에서 50배 및 100배 배율이였다. 본 발명 3종의 과립체 오디, 블루베리, 오미자 각각을 전자현미경으로 입자를 관찰하여 그 사진을 도 3에 정리하였다. 본 발명 3종의 과립체의 입자 표면은 부드러운 구형의 형태와 매끄러운 표면을 갖고 있다. 본 발명 오디 과립체의 입자 크기는 그 크기가 일정하고 개별적인 형태의 입자가 골고루 형성되어 있었으며, 본 발명 블루베리 과립체의 표면은 점착물질이 달라붙어 매끄럽지 않았다. 본 발명 오미자 과립체는 당의 영향에 의해 서로 결합한 형태의 모양을 나타내었다. (A-1),(B-1), (C-1)은 3.0KV ×50 으로 SEM을 측정하였고 (A-2),(B-2), (C-2) 3.0KV ×100 으로 SEM을 측정하였다. 100배의 비율로 측정한 결과를 보면 50배보다 구형의 형태와 표면이 더 자세히 관찰되었다.
The surface structure of each of the present invention Audi, blueberry, Schizandra granules was observed using a scanning electron microscope (scanning electron microscope, 160A, Shimazu, Japan) after gold ion coating, the microscope observation conditions were 50 times at 3.0kV And 100 times magnification. Each of the three granular bodies, blueberries, and schizandrae of the present invention were observed with an electron microscope, and the photographs are summarized in FIG. 3. The particle surface of the three granules of the present invention has a smooth spherical shape and a smooth surface. The particle size of the present invention granules was uniform in size and evenly formed particles of individual shapes, the surface of the present invention blueberry granules were not smooth due to the adhesion of the adhesive material. The schizandra granules of the present invention exhibited the shape of the form bound to each other under the influence of sugar. (A-1), (B-1), and (C-1) measured SEM at 3.0KV × 50, and SEM at (A-2), (B-2), (C-2) 3.0KV × 100. Was measured. Spherical shapes and surfaces were observed in more detail than 50 times when measured at the rate of 100 times.

3. 과립 색소 저장 안정성3. Granular pigment storage stability

오디, 블루베리, 오미자 각각 3종의 과립체 제조에 있어서 통상의 방법으로 제조한 과립체와 본 발명으로 제조한 3종의 과립체의 안토시아닌 색소에 대한 저장 안정성을 7일 간격으로 7주간 상온에 보관하면서 안토시아닌 함량을 측정하여 비교하였다.Storage stability of the anthocyanin pigments of granules prepared by the conventional method and three granules prepared according to the present invention for the three granules of Audi, blueberry, and Schisandra chinensis were kept at room temperature for 7 weeks at 7 days intervals. Anthocyanin content was measured and compared during storage.

<실시예 1>에 기재한 방법으로 얻은 오디, 블루베리, 오미자로부터 anthocyanin 색소를 함유한 각각의 추출물을 과립배합비율에 따라 2종의 과립체를 만들었으며 상기 오디, 블루베리, 오미자 각각의 추출물로부터 제조된 2종의 과림체에 대한 저장안정성을 평가하였다.Each of the extracts containing anthocyanin pigments from the mulberry, blueberry and schisandra chinensis obtained by the method described in Example 1 was prepared according to the granulation ratio. Storage stability was evaluated for the two kinds of fruiting bodies prepared from.

대조구로는 통상적인 방법으로 제조한 과립체 조성물(농축액 20%에 무수정제포도당 25%, 함수결정 45%, 유당분말 4.97%, 말토덱스트린5%, 향(우가와향)0.03%)로 하였으며, 본 발명 방법으로(농축액 20%에 무수정제포도당 23.5%, 함수결정 43%, alginic acid sodium salt 2%, 액상칼슘 1.5%, 유당분말 4.97%, 말토덱스트린5%, 향(우가와향) 0.03%) 제조한 과립체를 7주간 상온에 보관하면서 안토시아닌 함량을 비교 분석하여 도 4에 나타냈다. 도 4에 나타난 바와 같이 대조구의 경우 초기 오디, 블루베리, 오미자 각각의 과립체의 안토시아닌 함량은 각각 75.48 mg/100g, 74.88 mg/100g, 16.64 mg/100이었으나 7주간 저장 후 각각 55.68 mg/100g, 51.78 mg/100g, 4.86 mg/100g으로 감소하였다. 그러나 본 발명으로 제조된 3종의 과립체 오디, 블루베리, 오미자의 초기 안토시아닌 함량은 각각 76.26 mg/100g, 75.26 mg/100g, 16.70 mg/100g이었고 7주간 저장 후 측정한 안토시아닌 함량은 각각 69.47 mg/100g, 51.79 mg/100g, 10.02 mg/100g으로 대조구에 비해 안토시아닌 안정성이 뛰어나게 향상되었다.
As a control, the granule composition prepared in a conventional manner (20% concentrated liquid to 25% crude glucose, hydrous crystallized 45%, lactose powder 4.97%, maltodextrin 5%, flavor (Ugawa flavor) 0.03%), According to the method of the present invention (concentrated 20% in anhydrous glucose, 23.5%, hydrous 43%, alginic acid sodium salt 2%, liquid calcium 1.5%, lactose powder 4.97%, maltodextrin 5%, flavor (Ugawa flavor) 0.03% 7) The prepared granules were stored at room temperature for 7 weeks, and the anthocyanin content was analyzed and shown in FIG. 4. As shown in FIG. 4, the anthocyanin content of the granules of the initial Audi, blueberry, and Schisandra chinensis were 75.48 mg / 100g, 74.88 mg / 100g, and 16.64 mg / 100, respectively, after storage for 7 weeks, respectively, 55.68 mg / 100g, Decreased to 51.78 mg / 100 g, 4.86 mg / 100 g. However, the initial anthocyanin contents of the three granular bodies, blueberries, and schizandrae prepared by the present invention were 76.26 mg / 100g, 75.26 mg / 100g, and 16.70 mg / 100g, respectively. The anthocyanin contents measured after 7 weeks of storage were 69.47 mg, respectively. / 100g, 51.79 mg / 100g, 10.02 mg / 100g improved anthocyanin stability significantly compared to the control.

<< 실시예Example 5> 통계처리 5> Statistics

모든 실험결과는 SPSS(version 18.0, SPSS Inc., Chicago, IL, USA)를 이용한 분산분석(ANOVA)을 실시하였고 각 측정 평균값의 유의성(p<0.05)은 Duncan's multiple range test를 실시하여 검정하였다.
All the results were analyzed by ANOVA using SPSS (version 18.0, SPSS Inc., Chicago, IL, USA), and the significance (p <0.05) of each mean was tested by Duncan's multiple range test.

이상에서 설명한 바와 같이, 본 발명은 항암, 항당뇨, 항염증, 항고지혈증, 항산화 등의 생리활성을 갖는 오디, 블루베리, 오미자 추출물의 안토시아닌 색소의 저장 안정성이 크게 향상된 과립체 제조방법을 제공하는 뛰어난 효과가 있으므로 기능성 식품소재 산업 및 건강기능식품 산업상 매우 유용한 발명인 것이다.As described above, the present invention provides a method for producing granules having greatly improved storage stability of anthocyanin pigments of Audi, blueberry, and Schizandra chinensis extracts having physiological activities such as anticancer, antidiabetic, anti-inflammatory, antihyperlipidemia, and antioxidant activity. Because of the excellent effect is a very useful invention in the functional food material industry and health functional food industry.

Claims (6)

안토시아닌 색소성분을 함유한 시료를 건조하는 단계와; 추출용매를 제조하는 단계와; 상기 건조시료 1중량%에 대해 상기에서 제조한 추출용매 20중량%를 첨가하여 안토시아닌 색소가 함유된 추출물을 얻는 단계와; 상기에서 얻은 안토시아닌 색소함유 추출물을 여과를 통하여 불순물을 제거하는 단계와; 상기 여과물을 30 브릭스로 농축시키는 단계와; 과립체 100중량%에 대해 상기 농축액 20중량%와 과립체 제조용 혼합물을 제조하는 단계와; 상기에서 얻은 혼합물을 과립기에 통과시켜 과립체를 제조하는 것을 특징으로 하는 안토시아닌 색소의 저장 안정성이 향상된 과립체의 제조방법.Drying the sample containing the anthocyanin pigment component; Preparing an extractant; Obtaining an extract containing anthocyanin pigment by adding 20% by weight of the extraction solvent prepared above to 1% by weight of the dry sample; Removing impurities from the anthocyanin pigment-containing extract obtained by filtration; Concentrating the filtrate to 30 brix; Preparing a mixture for preparing the granules with 20% by weight of the concentrate relative to 100% by weight of the granules; A method for producing granules having improved storage stability of the anthocyanin pigment, characterized in that the granules are prepared by passing the obtained mixture through a granulator. 제 1항에 있어서, 상기 시료는 오디, 블루베리, 오미자 중에서 선택된 과립체 제조방법According to claim 1, wherein the sample is a granule manufacturing method selected from Audi, blueberry, Schizandra 제 1항에 있어서, 상기 추출용매는 추출용매 100중량%에 대하여 Citric acid 0.1중량% 및 HCl 0.5중량%을 함유하는 물 50중량%와 에탄올 50중량%를 혼합하여 제조하는 것을 특징으로 하는 추출용매를 사용한 과립체 제조방법The extracting solvent of claim 1, wherein the extraction solvent is prepared by mixing 50% by weight of water and 50% by weight of ethanol containing 0.1% by weight of Citric acid and 0.5% by weight of HCl, based on 100% by weight of the extraction solvent. Method for producing granules using 제 1항에 있어서, 상기 과립체 제조용 혼합물은 무수정제포도당 23.5 중량%, 함수결정 43 중량%, alginic acid sodium salt 2중량%, 액상칼슘 1.5중량%, 유당분말 4.97중량%, 말토덱스트린 5중량%, 향(우가와향) 0.03중량%의 비율로 혼합하는 것을 특징으로 하는 과립체 제조방법The mixture for preparing granules according to claim 1, wherein the mixture for preparing granules is 23.5% by weight of crude glucose, 43% by weight of hydrous crystals, 2% by weight of alginic acid sodium salt, 1.5% by weight of liquid calcium, 4.97% by weight of lactose powder, 5% by weight of maltodextrin. , Flavor (Ugawa fragrance) 0.03% by weight of a granule manufacturing method characterized by mixing 제 1항 또는 제 2항의 방법으로 안토시아닌 색소의 저장 안정성이 향상된 오디, 블루베리 또는 오미자 중 어느 하나의 과립체 The granule of any one of audi, blueberry or schisandra chinensis improved storage stability of the anthocyanin pigment by the method of claim 1 or 2. 제 5항 기재의 안토시아닌 색소의 저장 안정성이 향상된 과립체를 유효성분으로 함유하는 것을 특징으로 하는 건강기능성식품 조성물A health functional food composition comprising granules having an improved storage stability of the anthocyanin pigment according to claim 5 as an active ingredient.
KR1020120044536A 2012-04-27 2012-04-27 A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same KR20130121385A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020120044536A KR20130121385A (en) 2012-04-27 2012-04-27 A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020120044536A KR20130121385A (en) 2012-04-27 2012-04-27 A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020150036262A Division KR101601237B1 (en) 2015-03-16 2015-03-16 A granule improved stock stability of anthocyanin pigment from Mulberry, Blueberry and S. chinensis Baillon extracts and manufacturing method of the same

Publications (1)

Publication Number Publication Date
KR20130121385A true KR20130121385A (en) 2013-11-06

Family

ID=49851628

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020120044536A KR20130121385A (en) 2012-04-27 2012-04-27 A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same

Country Status (1)

Country Link
KR (1) KR20130121385A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103626733A (en) * 2013-11-08 2014-03-12 天津大学 Separation method of blueberry anthocyanin
KR101483727B1 (en) * 2013-11-11 2015-01-19 중앙대학교 산학협력단 Anti-inflammatory Composition Containing Pigment Extract from Morus alba L
KR20160025916A (en) * 2014-08-28 2016-03-09 (재) 경북천연염색산업연구원 Beverage compositions of the antioxidant and anti-Listeria capability containing natural plant material
CN105497205A (en) * 2014-10-15 2016-04-20 宁波大学 Medicine for interventionally treating diabetes

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103626733A (en) * 2013-11-08 2014-03-12 天津大学 Separation method of blueberry anthocyanin
KR101483727B1 (en) * 2013-11-11 2015-01-19 중앙대학교 산학협력단 Anti-inflammatory Composition Containing Pigment Extract from Morus alba L
KR20160025916A (en) * 2014-08-28 2016-03-09 (재) 경북천연염색산업연구원 Beverage compositions of the antioxidant and anti-Listeria capability containing natural plant material
CN105497205A (en) * 2014-10-15 2016-04-20 宁波大学 Medicine for interventionally treating diabetes

Similar Documents

Publication Publication Date Title
Brahmi et al. The efficacy of phenolics compounds with different polarities as antioxidants from olive leaves depending on seasonal variations
KR101682552B1 (en) Antioxidant composition comprising red ginseng extracts and berrylike mixtures
Bastos et al. Betalains as antioxidants
Ogunwa et al. Phytochemical evaluation and in vitro antioxidant status of Clerodendrum volubile (an indigenous medicinal plant)
KR20130121385A (en) A granule improved stock stability of anthocyanin pigment from mulberry, blueberry and s. chinensis baillon extracts and manufacturing method of the same
Puangpronpitag et al. Phytochemical screening and antioxidant activities of the seedling extracts from inca peanut plukenetia volubilis
Sriyab et al. Nutricosmetic effects of Asparagus officinalis: A potent matrix metalloproteinase-1 inhibitor
Krupa-Małkiewicz et al. Effect of nanosilver (nAg) on disinfection, growth, and chemical composition of young barley leaves under in vitro conditions
Helvacıoğlu et al. Comparatively investigation of grape molasses produced by conventional and industrial techniques
KR101601237B1 (en) A granule improved stock stability of anthocyanin pigment from Mulberry, Blueberry and S. chinensis Baillon extracts and manufacturing method of the same
Vifta et al. Nanoparticle from Medinilla speciosa with various of encapsulating agent and their antioxidant activities using ferric reducing assay
El-Saber Effect of biosynthesized Zn and Se nanoparticles on the productivity and active constituents of garlic subjected to saline stress
Guo et al. Antioxidant and antidiabetic activities of Ulmus davidiana extracts
Dimcheva et al. ANTIOXIDANT ACTIVITY AND POLYPHENOLIC CONTENT OF THE BULGARIAN WILD HERB Cistus incanus L. STORED UNDER DIFFERENT CONDITIONS.
JP2001299305A (en) Composition for scavenging active oxygen, and method for producing the same
Thanh et al. The effect of Roselle calyces extract on the chemical and sensory properties of cupcakes
Loucif et al. Metal chelating and cupric ion reducing antioxidant capacities of Ammoides atlantica aqueous extract
KR101389471B1 (en) Method for producing chlorella powder with increased chlorophyll content and antioxidant activity
KR102203209B1 (en) Method for granulation of plant powder containing antioxidant materials using Cryogenic Micro Grinding Technology
Rutnakornpituk et al. Antioxidant activity, total phenolic and flavonoid contents and analyses of active compounds in Stemona collinsae Craib crude extracts
Dobrin et al. Phytochemical research on aerial parts of Raphanus raphanistrum subsp. landra (Moretti ex DC.) Bonnier & Layens.
Koraqi et al. Effect of extraction solvent on bioactive compounds and antioxidant activity of Achillea millefolium L. grown in Kosovo region
EP4241779A1 (en) Aronia extracts and uses thereof
Ocampo et al. Content and antioxidant capacity of phenolic compounds in quinoa seed: A review
Mohamad et al. A Study on Antioxidant Properties of Eurycoma Longifolia (Tongkat Ali)

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
AMND Amendment
E601 Decision to refuse application
AMND Amendment
E90F Notification of reason for final refusal
AMND Amendment
A107 Divisional application of patent
J201 Request for trial against refusal decision