KR20160025916A - Beverage compositions of the antioxidant and anti-Listeria capability containing natural plant material - Google Patents

Abstract

The present invention relates to a beverage composition with a high anti-oxidative and anti-listeria function containing a natural plant material. More specifically, the beverage composition includes: 6 wt% of cranberry concentrate; 1 wt% of aronia concentrate; 25 wt% of MBA concentrate; 2 wt% of Schisandra chinensis fruit concentrate; 10 wt% of apple concentrate; 3 wt% of Rubus coreanus berry concentrate; 3 wt% of raspberry concentrate; 11 wt% of blueberry concentrate; 26 wt% of campbell early concentrate; 5 wt% of strawberry concentrate; 3 wt% of mulberry concentrate; 2 wt% of broccoli concentrate; 1 wt% of kale concentrate; and 2 wt% of natural oriental medicinal ingredient concentrate. Since the present invention bears excellent taste, anti-oxidation function, and anti-listeria function, it can be very useful for modern people who are constantly exposed to various types of stresses and oxidative stresses such as pollution and smoking.

Description

TECHNICAL FIELD The present invention relates to a beverage composition containing a natural plant material and having high antioxidant antirustic properties,

The present invention relates to a beverage composition having high antioxidative and antimicrobial activity, comprising a natural plant material, and more particularly to a beverage composition containing a natural plant material and having excellent antioxidant, To a beverage composition of high antioxidant antirustic acid function containing a natural plant material which is beneficial for health.

'Oxidation' refers to a reaction in which a substance combines with oxygen, which is a reaction that takes away hydrogen, a reaction that takes electrons from the ion (eg, Cl- → Cl), a reaction that increases the positive (+) charge ₂ + → Fe ₃ +) are all oxidation reactions. In vivo, dehydrogenation, oxidation, and electron transfer are carried out by oxygen, and oxidization by enzymes in the air, which plays an important role in metabolism and energy generation, Also called autoxidation, the quality of food is deteriorated by this automatic oxidation. For example, oxidation of lipids produces oxidized bums and peroxides.

This 'oxidation' occurs not only in food but also in the human body, and the human body is subject to 'oxidative stress' due to the rapid increase of harmful oxygen during the energy generation process of the human body (through the oxidation process in which the consumed food reacts with oxygen).

In other words, active oxygen (harmful oxygen) generated in the human body positively affects when proper position, time, and appropriate amount are generated in the human body, and also the toxic effect by the harmful oxygen can be solved However, our body regulates the amount of active oxygen in the body itself.

However, when the production of harmful oxygen increases sharply due to excessive exercise, excessive stress and ultraviolet ray, smoking and drinking water, environmental pollutants such as automobile exhaust gas, excessive food intake, and the like, Various diseases are induced. The side effect of harmful oxygen is called 'oxidative stress'.

The harmful free radicals generated by the oxidative stress cause the damage of the immune system. The harmful free radicals attack and change the antigenic determinant, so the immune cells can not distinguish between the foreign body and normal cells, do.

Such a mechanism causes autoimmune diseases (rheumatoid arthritis, diabetes, hair loss, etc.), and the natural healing power of the human body is decreased, thereby increasing the incidence of cancer. It also causes arteriosclerosis due to inflammation of blood vessels, coronary artery disease, and various cardiovascular diseases. In addition, harmful free radicals can cause aging of smooth muscle that moves blood vessels, gastrointestinal tract, and heart. Smooth muscles absorb nutrients and oxygen through the blood vessels, producing energy in the mitochondria and releasing waste products. However, when the smooth muscle ages, the mitochondria in the muscle cells induce oxidative aging, resulting in a decrease in energy production, leading to blood vessel inflammation and thrombogenesis, resulting in a rapid progression of aging due to a decrease in nutritional supply to muscles.

Therefore, in order to maintain a healthy life, it is important to suppress the 'oxidative stress' occurring in the human body. For this purpose, it is necessary to prevent the increase of active oxygen in the body through proper exercise and sufficient rest, It is desirable to reduce oxidative stress by ingestion.

'Antioxidant activity' refers to the oxidation inhibiting action. The catechins contained in large amounts in green tea are not only peroxidized in peroxides (for example, t-butyl hydroperoxide) in vivo but also reacted in vivo in vitro (Thiobarbituric acid reaction products, TBARS), and the antioxidant activity of antioxidants such as catechins of green tea has two hydroxyl groups (hydroxyl groups) in addition to catechins And vitamin C, vitamin E, and β-carotene are antioxidant vitamins because they have antioxidant properties.

Patent Registration No. 10-0578626 Patent Registration No. 10-0635529

As described above, green tea is widely known as an orally ingestible food having antioxidant function. It is widely known that the human body benefit of green tea is widely known, so that not only green tea which can be easily taken orally, And green tea ice cream.

However, while green tea has a beneficial effect on the human body, catechin contains a large amount of caffeine, which is an alkaloid contained in fruits, leaves and seeds of some plants such as coffee or common tea. If you take an appropriate amount of the central nervous system to work on the mind to awaken and reduce fatigue, while the effects of long-term high doses of 'caffeine addiction' can cause.

Commonly known caffeine addiction involves a variety of physical and mental symptoms including irritability, anxiety, nervousness, insomnia, headache, heart tremor, hyperreflexia, respiratory alkalosis, It is a problem that can cause gastric ulcer, erosive esophagitis and gastroesophageal reflux disease for a long time because it promotes the secretion.

Therefore, there is a need for the development of food having high antioxidant ability which is beneficial to the human body and capable of mass production, without the possibility of toxic diseases even if taken for a long time.

The present invention provides a beverage composition containing a natural plant material and having high antioxidative and antimicrobial activity, which comprises 6% by weight of a cranberry concentrate, 1% by weight of an Aronia concentrate, 25% by weight of an MBA concentrate, 2% by weight of Omija concentrate, 10% by weight of apple concentrate, 3% by weight of bokbule concentrate, 3% by weight of raspberry concentrate, 11% by weight of blueberry concentrate, 26% by weight of a campbell concentrate, 5% by weight of strawberry concentrate, 2% by weight of broccoli concentrate, 1% by weight of kale concentrate and 2% by weight of concentrated natural herbal material, Can be achieved.

The high-antioxidant beverage composition of the present invention containing the natural botanical material of the present invention is excellent in sensory properties as a beverage composition by mixing a concentrate of natural and fruit flavors having various colors and tastes, and has high antioxidant and anti-listerial function Therefore, it has a very beneficial effect on the health of modern people exposed to oxidative stress by external factors such as various stress, pollution, and smoking.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photomicrograph of photosensitizing damage measurement for measuring the light damage inhibiting ability of a root canal root extract contained in a beverage composition of high antioxidant antirusticia function containing a natural plant material of the present invention. FIG.
FIG. 2 is a photomicrograph of photomicrographs of genomic DNA for measuring the light damage inhibiting ability of a root canal root extract contained in a beverage composition of high antioxidant antirusticia function containing a natural plant material of the present invention.

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the structure and action of a beverage composition of high antioxidant antirusticia function containing a natural plant material of the present invention will be described in more detail with reference to the accompanying drawings.

The high-antioxidant anticorrosive beverage composition containing the natural plant material of the present invention contains 6 wt% of cranberry concentrate, 1 wt% of Aronia concentrate, 25 wt% of MBA concentrate, 2 wt% of Omiza concentrate, 10 wt% of apple concentrate, 3% by weight of raspberry extract, 3% by weight of raspberry concentrate, 11% by weight of blueberry concentrate, 26% by weight of cambellberry concentrate, 5% by weight of strawberry concentrate, 3% And 2% by weight of a concentrated liquid.

The cranberry (Vacinium macrocarpon) is a plant physiologically active substance, which is Flavan-3-ols, A-type PACs, Anthocyanins, Benzoic acid, Ursolic acid and the like. In particular, cranberry contains a large amount of proanthocyanidin A, which has been proven in numerous clinical trials to be beneficial to cardiovascular and immune systems and is therefore widely used as a anti-cancer adjuvant and cystitis preventative.

The Aronia melanocarpa is native to North America (USA, Canada), and its fruit and leaves are known to be effective enough to be used as traditional medicines by Indians and early American settlers.

It contains vitamins A, C, E, B2, B6, B9, B12, folic acid, quinic acid, phenolic acids, anthocyanin, tannin- Catechins, quercitin (good for angina), 02 nutritional ingredient routines (rutin-cholesterol lowering, hypertension treatment, good for arteriosclerosis), hesperidin (hypertension) ), Resveratrol (anti-aging), beta-carotene (good for eyes), and various organic minerals such as calcium, iron, magnesium, zinc, potassium and manganese.

It also contains good organic acids and many other phytochemicals. In particular, the tannin content of Aronia is rich in antioxidants and natural preservatives, and acts as an immune system against pathogens.

The above-mentioned Schizandra chinensis B. is an evergreen vine plant belonging to Magnoliaceae. It has five flavors of sweetness, sourness, bitter taste, spicy taste and salty taste and shows a clear red color by anthocyanin . Carbohydrates in the pulp of Omija and K, Mn and Ca in the seeds are abundantly contained. Especially, the fruit has been reported to be excellent in alcohol detoxification and antioxidation. It has been reported to have various physiological activities such as alcohol detoxification, hypoglycemia, cholesterol lowering, immunomodulation, anticancer and antitumor, and the main ingredient is lignan compound And palmitic acid and stearic acid, and schizandrin, schizadran, γ-schizadrin, ethamigrenal and gomisin have been reported.

The apple is an alkaline food with a low calorie content and a lot of good ingredients. Among them, typical dietary fiber sends out the harmful cholesterol accumulated in blood vessels and increases beneficial cholesterol to prevent atherosclerosis. Helps prevent and treat hypertension by releasing saliva from the body.

Pectin, a soluble fiber in apples, increases the viscosity of gastric juices and excretes malignant cholesterol to suppress the rapid increase in blood pressure. Phenolic acid neutralizes unstable harmful oxygen in the body to prevent stroke, It enhances pulmonary function and protects lungs from tobacco smoke and pollutants.

In addition, apples are rich in organic acids that remove fatigue substances and vitamin C, which is good for skin cosmetics, and are known to contain a large amount of polyphenols.

Polyphenols contained in apples contain various polyphenols in a certain ratio, and among them, they contain a large amount of floridines classified as flavonols, keletil and dihydrochalcone. The apple polyphenol is a representative antioxidant food having an excellent antioxidant function by directly acting on the active oxygen in the body, activating the human antioxidant system itself and increasing the expression of other genes involved in antioxidation.

Rubus coreanus is a deciduous shrub of Rubus, Rosales and Rosaceae. It is distributed only in Southeast Asia, especially in China, Japan, and the southern part of the Korean peninsula. Is reddish and new branches are covered with white powder on a green background. It blooms in May to June with light pink flowers. Fruits ripen in July to August, ripen in round and red color, but gradually become black. Traditionally, immature seeds have been used as medicines for the treatment of gangjeong, gyeongsin, ginsin, yin, and wool in oriental medicine, and they have been used for the treatment of seminal root, enuresis, asthma and allergy related diseases.

The anthocyanin pigment contained in the bokbunja is a water-soluble flavonoid pigment showing red, purple, and blue, and is a plant pigment that is differently expressed according to characteristics of plant parts. However, antioxidant, anti-inflammatory, anti-cancer, Peroxidation inhibition and DNA celavage protection.

Raspberry is a deciduous shrub that is cultivated for eating fruits such as European and North American species. The common components of raspberry are moisture 22.67%, crude protein 2.64%, crude fat 1.67%, soluble matter 70.19%, crude fiber 0.85% And ash of 1.98%.

In particular, raspberry contains 5.340 mg / g of phenolic compounds, and the major inorganic components contain 97.48 mg / 100 g of Ca, 215.20 mg / 100 g of K, 294.04 mg / 100 g of P, and 137.67 mg / 100 g of Na, The total amino acid content is 2,098.82mg / 100g, and the main amino acids are glutamic acid, aspartic acid and leucine.

The blueberry is a shrub plant belonging to the genus Eraccaaceae (Vaccinium) and has about 400 species and is distributed mainly in Southeast Asia. In North America, three species are cultivated as commercially important fruits such as V.corymbosum, V. angustifolium and V. ashei.

The general components of blueberry contain 10.47% of moisture, 2.66% of crude protein, 2.04% of crude fat, 81.36% of soluble substance, 1.48% of crude fiber and 1.99% of ash and total phenolic compound content is 9.028 ㎎ / g higher than raspberry .

In addition, the inorganic components such as raspberry contain 451.34 mg / 100 g of Ca, 355.40 mg / 100 g of K, 321.10 mg / 100 g of P and 137.58 mg / 100 g of Na and the total amino acid content of the blueberry is 2,011.44 mg / Contains glutamic acid, aspartic acid and leucine.

Blueberries are processed as jams, wines, sauces, etc. in addition to raw materials, and they are widely used as raw materials for confectionery. They are known to have powerful functions such as antioxidation, visual enhancement, antidiabetic and anti-cancer effects. This is a big increase.

The Campbell Early is the grape variety having the largest cultivated area in Korea, and it is known that it has good flavor and distinctive flavor and good merchantability. Grapes (Vits vinifera L.) are Rhamnale and Vitaceae crops. Fruits are mainly used for reproduction, raw materials for the production of processed foods such as juice, wine, vinegar and seeds. Processed and used. More than 70% of the total production is consumed for reproduction, and 30% is consumed for processing beverages, wine, and other products. Beneficial components such as polyphenols, anthocyanins, and resveratrol in grapes have been proven to have anticarcinogenic effects in killing cancer cells as well as atherosclerosis and anti-diabetes.

Strawberry is a perennial plant of rosemary rosaceae which is a perennial herbaceous plant with a calorific value of only 27kcal per 100g. It is effective for dieting. It is rich in vitamin C and has excellent antioxidant activity. When it is taken with lactobacillus product, calcium is supplemented. It is known to help prevent osteoporosis.

The above-mentioned mulberry is a mature fruit of a deciduous tree, black or magenta, collected from May to June, and used for food or as a herb medicine. Mature audi has a large amount of anthocyanin-based pigments and contains higher nutrients than ordinary fruits. In particular, vitamin C has been reported to be 1.5 times higher than citrus fruits, and it contains a large amount of free sugars such as fructose and glucose, a small amount of oxalic acid and citric acid,

Broccoli (Broccoli) is a dark green vegetable belonging to mustard, also called 'green flower cabbage'. Broccoli is one of the most used vegetables for Western food such as salads, soups and stews.

Broccoli is rich in antioxidants such as vitamin C and beta-carotene. Beta-carotene is a micro-nutrient that has antioxidant activity as a pre-production substance of vitamin A. Vitamin C, which helps absorption of large amounts of calcium and calcium in broccoli, helps prevent osteoporosis. In addition, it has been known that it has an effect of inhibiting cancer cell activation and cancer cell proliferation because it contains abundant sulfur compounds having strong antitumor activity.

The Kale is known as the food having the highest content of beta carotene among the greenish-yellow vegetables. The main ingredients include chlorophyll, calcium 407mg, phosphorus 49mg, iron 5.8mg, vitamin A 4,500IU, fiber, calcium, vitamin B1, vitamin B2, vitamin C, E, K and so on, It is known that it has an excellent effect on treatment, and it is known to be effective for the production of new cells.

Wherein the concentrated natural herbal material concentrate comprises a natural herbarium material comprising 0.5% by weight of an aqueous extract of Hosui, 1% by weight of an Aspergillus oryzae extract, 8% by weight of a grape stem extract and 4% by weight of a grape seed extract; And 86.5% by weight of water, which is the sum of the water generated from each extract in the course of extracting the natural herbal ingredients.

Polygonum multiflorum is a Chinese native perennial herbaceous perennial plant native to China. It has been used for many years as a supplementary, supplementary, hypervigilant, (悅子), 정 鬚 (精 鬚 鬚), 悅 鬚 鬚 (悅 鬚 鬚), 服 服 服 (悅 鬚 鬚) ): It has been used as a medicinal product with efficacy that has an efficacy that is effective for the treatment of yew pure water, jangguk bone (long bones) and yearly burn injury. Recently, it has been used for the treatment of cholesterol lowering, atherosclerosis inhibition, It is a representative herbaceous plant known to have the efficacy of small scale.

On the other hand, Korean Patent Publication No. 10-1424175 and Korean Patent Laid-Open Publication No. 2001-0077956 disclose data demonstrating the effect on the antioxidative function of one of the main additives of the present invention, The description of experimental results necessary for demonstrating antioxidant functional properties is omitted.

Rumex crispus L. is a perennial herbaceous perennial plant that grows throughout Korea and has a height of 30-80cm. Its stems grow straight. The leaves are alive. The petiole is short and the petiole is short and narrow with both ends and wrinkles.

R. japonicus HOUTT, R. conglomeratus MURP., And R. maritimus L., which are morphologically similar to the cryptograss species, also live in Korea. In the private sector, leaves are used for edible purposes, and the leaves and roots of the spikelets are used as various prescription drugs such as hemostasis, penetration, detoxification, bronchitis and constipation.

Recently, studies on the physiologically active substances and their physiological functions contained in the horsetails have been carried out. The extracts of Syrup Seeds have been reported to have anti-inflammatory analgesic and liver protective effects, and antibacterial, antifungal and anti-inflammatory activities have been reported. It has been reported that the root, seed or overhead extracts of DPPH showed DPPH radical scavenging activity and singlet oxygen scavenging activity similar to butylated hydroxyanisole (BHA) or ascorbic acid. In addition, the suppression of lipid peroxidation was also reported in the extracts of Cryptomeria japonica. Antioxidant substances such as 2,6-dichloro-4-nitrophenol, 2-isopropyl-5-methylphenol, 4-vinyl-2-methoxyphenol and 2,3-dihydrobenzofuran are known to exist.

Therefore, the following experiment was carried out to confirm antioxidant ability of Cryptomeria japonica.

The extracts were filtered through a filter paper to remove solids, and the 70% ethanol extract was washed with a vacuum concentrator (EYELA N1000, manufactured by Nisshin Chemical Industry Co., Ltd.) and dried in a vacuum oven for 5 days. Tokyo, Japan). After adding a certain amount of water to the extract obtained, the volume was adjusted to 1 L, and then each fraction was obtained by adding 1: 1 ratio of nucleic acid, chloroform, ethyl acetate and butanol as extraction solvents having different degrees of polarity.

First, add the same amount of the nucleic acid to the extract as the syrup extract, mix well and divide into two layers, water layer and extraction solvent. The nucleic acid solvent layer (HE) was concentrated separately, and the aqueous solution layer was again partitioned into chloroform (CE), ethyl acetate (EE) and butanol (BE).

After separating the butanol, the remaining aqueous layer was lyophilized, methanol (10 ml) was added and centrifuged. At this time, the supernatant was dissolved in methanol extract (ME) and the precipitate was dissolved in distilled water and used as a water extract (WE). Each extract was concentrated to 100 mg / ml in consideration of solubility, and stored at 4 ° C for use in the experiment.

Duksan (GR grade) and Sigma-Aldrich Co. (St. Louis, MO, USA) were used as solvents and reagents.

[Experimental Example 1]

<Total phenol content analysis>

Folin-Ciocalteu method (17) was used to evaluate the total polyphenol content of the root extracts (HE, CE, EE, BE, ME, WE) extracted with various solvents.

100 μl of the sample, 50 μl of Folin-ciocalteu reagent and 850 μl of 20% Na ₂ CO ₃ were mixed and stored in a dark room for 2 hours. The mixture was analyzed by a spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan) Respectively. The total phenol content was calculated using gallic acid as a standard calibration curve, and the total polyphenol content was calculated as mg gallic acid in 1 mg of the sample. The concentration of gallic acid was 10 ~ 500 ㎎ / ㎖, and the absorbance was evaluated at 765 nm by the above method.

Phenolic compounds are widely distributed in nature and are known to exhibit antioxidative activity. Table 1 shows the results of analysis of the total phenolic contents (TPC) of the various rootstock extracts (HE, CE, EE, BE, ME and WE)

As shown in Table 1, the TPC of the respective solvent extracts ranged from 0.083 to 0.664 mg GA / mg extract, and the ethyl acetate extract (EE) was 0.664 mg GA / mg extract, which was significantly higher than other solvent extracts . In addition, 0.288 mg GA / ㎎ extract was obtained for the chloroform extract (CE), 0.294 mg GA / ㎎ extract for the butanol extract (BE) and 0.291 mg GA / ㎎ extract for the nucleic acid extract (HE) (ME) showed 0.091 ㎎ GA / ㎎ extract and distilled water (WE) showed 0.083 ㎎ GA / ㎎ extract. In general, the content of phenolic compounds showed a positive correlation with antioxidant activity, and the antioxidant activity of the roots of Pseudomonas sp.

[Experimental Example 2]

<2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical measurement>

DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity was evaluated according to the method of Brand-Williams et al. After dissolving 6 × 10 ^-5 MDPPH in methanol, 900 μl of the DPPH radical solution and 100 μl of the sample were mixed and reacted for 5 minutes. Then, the absorbance was evaluated at 517 nm using a spectrophotometer (UV-1800, Shimadzu).

The scavenging ability of the DPPH radical was calculated by the following equation based on the change of the absorbance after the addition of the control group and the sample to which no addition was made.

The DPPH radical scavenging ability (electron donating ability,%) was plotted according to the concentration of the sample, and the sample concentration at the electron donating ability of 50% was expressed by 50% inhibition concentration (IC ₅₀ ).

DPPH has chemically stabilized free radicals and is reduced by ascorbic acid, tocopherol, and polyhydroxy aromatic compounds, resulting in discoloration of deep purple color, which is widely used to detect antioxidants from various natural materials (23). Table 2 shows the results of electron donating ability of roots extract.

The IC ₅₀ values indicating DPPH radical scavenging activity were 0.057 mg / ml of WE, 0.077 mg / ml of ME, 0.015 mg / ml of BE, 0.005 mg / ml of EE, 0.039 mg / ml of CE and 0.093 mg / The DPPH scavenging activity of the extract was excellent. Especially, it showed high DPPH radical scavenging activity in ethyl acetate extract (EE) and butanol extract (BE). It has a remarkably high antioxidant ability compared to 0.010 ㎎ / ㎖ of ascorbic acid Respectively. In addition, chloroform extract (CE) and water extract (WE) showed relatively good activity, while nucleic acid extract (HE) and methanol extract (ME) showed relatively low radical scavenging activity.

[Experimental Example 3]

<ABTS radical decolorization measurement>

The antioxidant capacity of ABTS [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt] radicals was measured by ABTS radical cation decolorization method. 7 mM ABTS and 2.4 mM potassium persulphate The ABTS radicals were formed in the dark and stored in the refrigerator. The radical stock solution was diluted with ethanol such that the absorbance value was 0.70 (± 0.02) at 734 nm. To 0.9 ml of this solution was added 0.1 ml of each sample solution prepared for each concentration, followed by reaction at room temperature for 7 minutes. Then, the absorbance change of the reaction solution was measured at 734 nm. The degree of ABTS radicalcation decolorization was expressed by the absorbance reduction rate of the sample solution addition group and the no addition group.

When ABTS and potassium persulfate are left in the cow, the ABTS radical is produced. The ABTS radical is cleared by the antioxidant power of the extract, and the radical specific cyan color is discolored. As described above, the ABTS radical decolorization reaction is a method of measuring the elimination activity of the ABTS radical by indicating the degree of elimination of the free radicals that have already been generated, and the ABTS radical decolorization reaction is completed within 1 minute. Therefore, the measurement can be performed in a short time, and both the hydrophobic and hydrophilic Lt; / RTI &gt;

The results of ABTS radical scavenging activity of Aspergillus niger root extract and ascorbic acid are shown in Table 3.

As shown in Table 3, the ethyl acetate extract (EE) of 0.36 쨉 g / ml showed a high ABTS radical scavenging activity of about 94% and an IC ₅₀ value of 0.17 / / ml. In addition, the IC ₅₀ value of the butanol extract (BE) was relatively high at 21.42 ㎍ / ㎖, and the IC ₅₀ values of other fractions were relatively low (32.4 ~ 133.4 ㎍ / ㎖). In the case of the nucleic acid fraction, ABTS radical scavenging The activity could not be confirmed. It was found that the ethyl acetate extract (EE) used in the experiment had a very high ABTS radical scavenging activity when the IC ₅₀ value of ascorbic acid, well known as an antioxidant, was measured to be 3.35 ㎍ / ㎖. In addition, the DPPH radical inhibitory activity tends to be higher than the ABTS radical inhibition activity, because the ABTS radical is a stronger oxidizing substance than the DPPH radical.

[Experimental Example 4]

<Measurement of single oxygen>

The imidazole-RNO bleaching method (20) was used to measure the single enzyme oxygen generated by the photo-sensitization reaction of rose bengal (RB). Univariate analysis was performed by measuring the absorbance change at 440 nm before and after exposure to 20 μM Tris-succinate buffer (pH 6.5) solution in which 2 μm rose bengal, 5 mM imidazole and 4 μm RNO were dissolved. Samples were exposed to white light (λ> 400 ㎚, 100 W · m-2) from a 150 Whalogen-lamp (Osram, Augsburg, Germany) for 4 minutes and all analyzes were carried out at 25 ° C. L-ascorbic acid was used as a control.

In order to examine the effect of single-oxygen inhibition on various rootstock extracts, the inhibitory effect of single-oxygen induced by type-II photosensitization was analyzed. Table 4 shows the effect of single-oxygen inhibition by treatment with the concentration of the sample.

The QC ₅₀ value of EE was 0.365 mg / ml, and BE was 0.464 mg / ml, indicating high antioxidant activity as previously described. ME was 2.481 mg / mL, and WE was 2.743 mg / mL. In the case of CE and HE, it was not effective. As a control group, ascorbic acid (AA), which is well known as an antioxidant, was used. In the case of AA, the QC ₅₀ value was 0.086 mg / ml.

[Experimental Example 5]

&Lt; Measurement of light damaging ability of living organism &

In this experiment, we investigated the inhibition of E. coli death by DNA damage and the inhibition of DNA damage by the single enzyme oxygen. Suh et al. (21). Escherichia coli Luria-bertari solid medium, sterilized paper disk, and photosensitizer (10 μg rose bengal) were used for the inhibition of E. coli death.

E. coli stained E. coli. After the sterilized paper disk was placed on the coli Luria-bertari solid medium, the untreated group, the photosensitizer treated group and the photosensitizer + sample (extract or ascorbic acid) were treated, exposed to light at 25 ° C for 20 hours, (Growth inhibition size) were observed. To confirm the ability of DNA damage inhibition, the extent of DNA breakage was determined according to the presence or absence of extract under light exposure conditions.

Experimental strain E. Genomic DNA was isolated from E. coli using an Exgene ™ cell mini kit (Gene All®). To the separated genomic DNA, 50 μL of RB solution was added to the separated genomic DNA, After investigation, genomic DNA breakdown was confirmed by 1% agarose electrophoresis.

The excellent antioxidant activity in the untreated extract shows that the ability to remove active oxygen generated when exposed to strong direct sunlight including ultraviolet light is excellent. First, the photoprotection effect of EE and BE extracts, which exerted a single-oxygen inhibitory effect, was investigated in order to examine the role of the scarlet roots extract from Escherichia coli damage caused by reactive oxygen species generated by Type-II photosensitization. Respectively.

Treated group, photosensitizer treated group, and photosensitizer + sample (extract or ascorbic acid) treated groups were exposed to light, and the growth state (growth inhibition size) of the coliform bacteria around the paper disk was observed. The growth of Escherichia coli was severely inhibited as shown in Fig. 1 when judged by the size of the growth inhibition zone formed around the paper disk due to the singlet oxygen generated when the photosensitizer rose bengal was exposed to light.

On the other hand, when ascorbic acid, EE, and BE were added, the size of growth inhibition rings decreased, suggesting that they suppressed or eliminated free radicals, which may have contributed to the survival of E. coli.

DNA damage inhibition test was carried out at the genetic level in order to examine the role of Root Extracts from the destruction of living organisms by ultraviolet rays and strong direct sunlight. The degree of DNA damage according to the lane 1, 2 and light exposure (lane 3-6) and the degree of DNA protection according to the presence of corydalis EE addition (lane 2, 4, 6) As a result, as shown in FIG. 2, it was confirmed that DNA damage was less in EE-added 4 and 6 samples than in 3 and 5-exposed samples without adding EE. It was found that EE, a root extract, suppresses DNA damage caused by active oxygen generated under light conditions.

In conclusion, the total phenolic content, DPPH, ABTS and single-enzyme oxygen analysis of roots extract showed excellent antioxidative activity in ethyl acetate extract and butanol extract in all experiments. It is judged that it will play an important role in protecting life from the light damage of a living organism.

On the other hand, in Korean Patent Publication No. 10-0533869 and Korean Patent Laid-Open Publication No. 10-2004-0092952, data proving effects on antioxidative function of grape seed, which is one of the main additives of the present invention, The description of the experimental results necessary for demonstrating the antioxidant functional properties of the grape seeds is omitted.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is clearly understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the invention as defined by the appended claims. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

Claims (2)

6% by weight of cranberry concentrate, 1% by weight of Aronia concentrate, 25% by weight of MBA concentrate, 2% by weight of Omiza concentrate, 10% by weight of apple concentrate, 3% by weight of brambled concentrate, 3% by weight of raspberry concentrate, A natural herbal material concentrate containing 26% by weight of a concentrated concentrate of Campbell, 5% by weight of a strawberry concentrate, 3% by weight of an audi concentrate, 2% by weight of a broccoli concentrate, 1% by weight of a kale concentrate and 2% A high-antioxidant anti-listeria beverage composition.
The method according to claim 1,
Wherein the concentrated natural herbal material concentrate comprises a natural herbarium material comprising 0.5% by weight of an aqueous extract of Hosui, 1% by weight of an Aspergillus oryzae extract, 8% by weight of a grape stem extract and 4% by weight of a grape seed extract;
Wherein the composition contains 86.5% by weight of water generated in the course of extracting the natural herbal ingredients.

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