KR102203209B1 - Method for granulation of plant powder containing antioxidant materials using Cryogenic Micro Grinding Technology - Google Patents

Abstract

The present invention relates to a method of maintaining the antioxidant activity of the original substance and improving the absorption rate in the body when a plant having antioxidant activity is pulverized and powdered and granulated by using a cryogenic micro-grinding technology (CMGT). . According to the present invention, by minimizing the destruction of nutrients while pulverizing plants with antioxidant functions into ultra-fine sizes of 10 to 50 μm at cryogenic temperatures of -196 to -70°C, the components are maintained as much as possible and absorption rate in the body is improved. Has the effect of being. In addition, it is possible to granulate while maintaining the active ingredients of antioxidant plants that are weak to heat through the low-temperature granulation process, thereby increasing dispersibility and improving the usability as processed products.

Description

Method for granulation of plant powder containing antioxidant materials using Cryogenic Micro Grinding Technology

The present invention relates to a method of maintaining the antioxidant activity of the original substance and improving the absorption rate in the body when a plant having antioxidant activity is pulverized, powdered and granulated by using a cryogenic micro-grinding technology (CMGT). will be.

The UN Future Report predicts that the world's population will reach 9 billion by 2050, which will intensify the food shortage, and FAO predicts that food shortages will continue or increase hunger and malnutrition. The decrease in the quantity and quality of food affects not only food security but also a balanced supply of nutrition. On the other hand, when ingesting nutritious food materials, as the digestion and absorption capacity decreases and the utilization rate in the body decreases, food development technology with high absorption capacity in vivo is required, and for this purpose, interest in cryogenic ultra-fine grinding technology is increasing.

Cryogenic ultra-fine grinding technology has been studied for the preservation of volatile components or fatty oil content of spices such as pepper, coriander, cumin, fenugreek, etc.(Singh & Goswami, 1999; Murthy & Suvendu, 2008; Bhupendra & Goswami, 2016; Sharma et al., 2016). Freeze-crushing technology is a technology that blocks heat generated during pulverization using liquid nitrogen at -196°C to prevent loss of flavor and nutrients in food due to heat (Ishito et al., 2002; Rohit et al., 2013; Saxena et al., 2015). Freeze crushing technology protects heat-sensitive ingredients and pulverizes substances evenly to maintain flavor evenly.Since it is pulverized at low temperature using liquid nitrogen, it has the effect of preventing thermal denaturation of proteins, etc., and preventing oxidation of lipids. (Krejеovаa et al., 2008; Bhupendra & Goswami, 2016). In addition, if it is prepared as an extract or a concentrate, only soluble ingredients can be ingested, but the cryogenic ultra-fine grinding technology is a technology that allows you to consume the original nutrition as it is, as it is manufactured into ultra-fine powder that can consume the raw material.

Meanwhile, as interest in functional foods and high-functional crops that can be regulated in vivo has increased, functional studies such as barley sprout, turmeric, hwangchil, and mushroom mushrooms are being conducted by applying various processing techniques.

Accordingly, the present inventors have completed the invention of increasing the permeability and bioavailability by pulverizing a plant having an antioxidant function into an ultra-fine powder while maintaining the antioxidant function of the original material using a cryogenic ultra-fine pulverization technology. Furthermore, a method of producing ultra-fine pulverized granules having an antioxidant function was completed by mixing and low-temperature granulation of the ultra-low-temperature ultra-fine pulverized powder and the cryogenic ultra-fine pulverized powder containing moisture without going through the drying process after cryogenic ultra-fine grinding.

The object of the present invention is (a) preparing an antioxidant plant consisting of turmeric, ginger and aronia; (b) the antioxidant plant selected from the group consisting of turmeric, ginger and aronia was frozen at -196°C to -70°C and pulverized to an average particle size of 10 to 50µm, and the pulverized product was -30 to -10 Drying at °C to prepare a first fine powder; (c) Second fine powder by freezing the antioxidant plant selected from the group consisting of turmeric, ginger and aronia prepared in step (a) at -196°C to -70°C and pulverizing to a size having an average particle size of 10 to 50 μm Manufacturing a; (d) mixing the first fine powder and the second fine powder; And (e) to provide a method for producing antioxidant plant granules comprising the step of granulating the mixture at 25 to 35 ℃.

In order to achieve the above object, (a) preparing an antioxidant plant consisting of turmeric, ginger and aronia; (b) the antioxidant plant selected from the group consisting of turmeric, ginger and aronia was frozen at -196°C to -70°C and pulverized to an average particle size of 10 to 50µm, and the pulverized product was -30 to -10 Drying at °C to prepare a first fine powder; (c) Second fine powder by freezing the antioxidant plant selected from the group consisting of turmeric, ginger and aronia prepared in step (a) at -196°C to -70°C and pulverizing to a size having an average particle size of 10 to 50 μm Manufacturing a; (d) mixing the first fine powder and the second fine powder; And (e) it provides a method for producing antioxidant plant granules comprising the step of granulating the mixture at 25 to 35 ℃.

In addition, (a) preparing an antioxidant plant consisting of turmeric, ginger and aronia; (b) freezing the antioxidant plant selected from the group consisting of turmeric, ginger and aronia at -196°C to -70°C and pulverizing it to a size having an average particle size of 10 to 50 μm; (c) It provides a method of producing an antioxidant plant powder comprising the step of drying the pulverized product at -30 to -10 ℃.

In the present invention, the antioxidant plant granules and powder may be characterized in that they maintain the polyphenol content of the raw material and have antioxidant activity.

In addition, the antioxidant plant granules and powders may be characterized in that the absorption rate in the body increases.

In the antioxidant plant granules according to the present invention, the first fine powder is 80 to 98% by weight, the second fine powder is 2 to 20% by weight, and the mixture of the first fine powder and the second fine powder is 5 to 20% by weight. It may be characterized by containing moisture. The mixing of the first fine powder and the second fine powder for granulating antioxidant plants may be characterized in that they are mixed in an airflow manner in a zero gravity state.

In the present invention, the "Cryogenic Micro Grinding Technology (CMGT)" is a technology that directly pulverizes the antioxidant plants into fine powders in the state of hardly freezing the antioxidant plants at cryogenic temperatures of -196°C to -80°C by adding liquid nitrogen to be. In general, if the raw material is pulverized without drying, it cannot be pulverized because of the moisture contained in the raw material. Even after the drying step, the crushed product is crushed like a gel due to the remaining moisture unless it is completely dried. Is obtained. On the other hand, the cryogenic ultra-fine pulverization method is to freeze and crush the raw material while maintaining the cryogenic state by supplying liquid nitrogen by immersing the raw material containing moisture in liquid nitrogen and freezing it. It is characterized in that by pulverizing at, complete powdering is possible even if it contains moisture, and ultrafine powder in micro units can be obtained.

In the present invention, PAMPA (Parallel Artificial Membrane Permeability Assay), which is used to check the permeability of nutrients, is a rapid in vitro model for passive intercellular permeability evaluation in vivo. Speak of technology.

In the present invention, "CMGT-mixed" refers to a fine powder (first fine powder) obtained by ultra-low temperature pulverization at a particle size of 10 to 50 μm at -196°C to -70°C and then drying at a low temperature at 10 to 30°C and -196 It refers to a mixture of fine powder (second fine powder) containing moisture obtained by ultra-low temperature pulverization at a particle size of 10 to 50 μm at ℃ to -70 ℃. 80 to 98% by weight of the first fine powder and 2 to 20% by weight of the second fine powder may be mixed, and the moisture content of the mixed fine powder may be 5 to 20%. The suggested ratios for the first and second fine powders are also mixed, and the first dispersion is performed on the mixture through powder sizing. At this time, since the second fine powder is a clustered type of particles containing moisture, the particles are dispersed in an airflow mixing method in a zero gravity state and mixed and exchanged with the first fine powder. At this time, if there is no self-cohesive force according to the characteristics of the raw material, a binder is required in addition to the moisture content, so when mixing with airflow, a basic excipient can be added to increase the degree of cohesion of the dispersed raw material, and the amount of basic excipient used can be up to 1 to 90% Since the use of one antioxidant plant is the main purpose, it is preferably used within 1 to 70%, more preferably within 1 to 30%. The basic excipients for reinforcing cohesion are dextrins (dextrin, maltodextrin, indigestible maltodextrin, isomaltoldextrin, etc.), crystalline cellulose, chicory dietary fiber powder, lactose, oligosaccharide powders (fructooligosaccharide, isomaltooligosaccharide, etc.), etc. You can use

In the present invention, "CMGT-granules" are formed by mixing the first fine powder and the second fine powder containing moisture, and then performing a low-temperature granulation process at 25 to 35°C. On the other hand, the general FBG granulation process requires a temperature condition of 45 to 60 °C and the lowest temperature condition is also 40 to 45 °C is required, so that the general granulation process may destroy the antioxidant components contained in the antioxidant plants.

However, in the granulation process according to the present invention, by mixing the second fine powder containing moisture, the moisture input time during granulation can be reduced due to an increase in the moisture content, so that the overall granulation operation time is shortened, and thus, antioxidants vulnerable to heat. Ingredients can be protected, and as the internal temperature of the contents decreases, the starting temperature of the granulation operation itself becomes low, so even if the temperature of the contents is increased by initial heating for granulation, granulation can be performed at low temperature. In addition, in the case of high-temperature granulation, the required amount of moisture is small, and in the case of low-temperature granulation, a relatively large moisture content and an appropriate temperature are required.According to the present invention, the degree of granulation is accelerated by reaching an appropriate moisture content in a short time. Has the effect of being.

As shown in FIG. 7 of the present invention, the fine powder generated through the drying process after CMGT 1 pulverization is referred to as "first fine powder", and the fine powder in a state that contains moisture without going through the drying process after CMGT 2 pulverization It was called "second fine powder".

In the present invention, the term "granule" is a fine powdery dry food that absorbs moisture when placed in a suitable humidity, and the powder component becomes sticky, and the powder agglomerates to form 30 to 150 times larger particles. Say. By granulation, the shape becomes a porous structure, which improves wettability, and at the same time increases the rate of dispersion and sedimentation in water. When granulated, the dispersibility is higher than that of the non-granulated powder and the usability as a processed product is excellent.

In the present invention, turmeric is a plant of the ginger family Turmeric ( Curcuma longa Radix ), Onulgeum ( C. aromatica ), Guangseo Achul ( C. kwangsiensis ), Bong Achul ( C. zedoaria ). Compared to normal turmeric a lot, turmeric is a rootstock, whereas turmeric is dried using tubers. It grows or grows in Southeast Asia, including southern China, India, and Okinawa, and is also cultivated in the central and southern regions of Korea. Curcumin, an indicator component of turmeric, is a type of alkaloid mainly contained in turmeric produced in India. It is also a type of curcuminoid. Curcuminoids are known to give off the yellow color of turmeric.

In the present invention, 6-gingerol, an index component of ginger, is one of the spicy ingredients of ginger, and plays a role in reducing lipids in the body, inhibiting antibacterial effects, suppressing tumors, and inhibiting DNA damage. It also helps reduce migraines and reduces vomiting caused by pregnancy or motion sickness.

In the present invention, anthocyanin, an indicator component of aronia, refers to a pigment mainly included in flowers or fruits, and is a substance that exhibits red, purple, blue, etc. depending on the concentration of hydrogen ions. Anthocyanins are known to have antioxidant effects.

In the present invention, CMGT grinding, mixing, and granulation experiments were performed using turmeric, ginger, and aronia as plants having antioxidant properties, but the CMGT grinding conditions, the mixing ratio of the first fine powder and the second fine powder, and granulation were determined. Including the features according to the present invention such as temperature conditions for turmeric, ginger, aronia and other antioxidant plants will be able to obtain the same effect according to the present invention. Antioxidant plants include barley sprouts, wheat sprouts, upper leaves, hwangchil, gooseberries, other berries, phylum mushrooms, and chaga mushrooms.

According to the present invention, by minimizing the destruction of nutrients while pulverizing plants with antioxidant functions into ultra-fine sizes of 10 to 50 μm at cryogenic temperatures of -196 to -70°C, the components are maintained as much as possible and absorption rate in the body is improved. Has the effect of being. In addition, it is possible to granulate while maintaining the active ingredients of antioxidant plants that are weak to heat through the low-temperature granulation process, thereby increasing dispersibility and improving the usability as processed products.

In addition, the powder or granules of the antioxidant plant according to the present invention can be used for the purpose of enhancing the function of health functional foods, and can be commercialized as patient food, elderly food, baby food, nutritional food, space food, diet food, and antioxidant supplement food. Depending on the purpose of supplying antioxidants, 2 to 90% of the powder or granules according to the present invention are contained, and powders, granulated granular products, pills, bars, liquid products, hard capsules, soft capsules, tablets, etc. It can be used as a type of product.

1 is a result of analysis of the particle size of CMGT-mixed turmeric and the disintegration particle size of CMGT-granules.
Figure 2 is a result of analysis of the particle size of CMGT-mixed and the disintegration particle size of CMGT-granules of Aronia.
3 is a result of analysis of the particle size of CMGT-mixed ginger and the disintegration particle size of CMGT-granules.
4 is a gallic acid standard curve used to confirm the total polyphenol content.
5 is a Trolox standard curve used to confirm the ABTS radical scavenging efficacy.
6 is a result of confirming the ABTS radical scavenging ability of the general pulverized product of turmeric and the CMGT pulverized.
7 is a result of confirming the ABTS radical scavenging ability of a general pulverized product of ginger and a pulverized CMGT.
8 is a result of confirming the ABTS radical scavenging ability of the general pulverized product of Aronia and the pulverized CMGT.
9 shows the UV chromatogram of the general pulverized product of ginger and the pulverized CMGT.
Figure 10 shows the CMGT-granulation process.
11 shows the dispersion index of CMGT-granules applied to FBG.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Preparation of ingredients

Turmeric, ginger and aronia were prepared as materials used in this experiment. The plants were dried and pulverized, and the lyophilized and lyophilized CMGT pulverized product obtained by cryogenic micro grinding technology (CMGT) was used as samples.

The indicator component of turmeric was curcumin, the indicator component of ginger was 6-gingerol, and the indicator component of aronia was anthocyanin. Total polyphenol content and antioxidant activity were measured for turmeric, ginger, and aronia, and cell membrane permeability was measured for all samples by using PAMPA (Parallel Artificial Membrane Permeability Assay).

Method for measuring polyphenol content

The polyphenol content of the extract was measured by the method of Folin-Denis. 0.5 ml of 50% Folin-Ciocalteu's phenol reagent was added to 1 ml of the extracted sample solution, and reacted at room temperature for 3 minutes. In the reaction solution, 1 ml of a saturated Na ₂ CO ₃ solution and 7.5 ml of distilled water were sequentially mixed, allowed to stand for 30 minutes, and the absorbance was measured at 760 nm. The total polyphenol content was calculated according to the calibration curve (y = 6.4072x + 0.0326) prepared using gallic acid as a standard material. The measurement unit was GAE (Gallic acid equivalent)/g.

ABTS radical scavenging assay method

The ability to remove 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS+ㆍ) was measured using an Antioxidant Assay Kit (CS0790, SigmaAldrich, USA). As a standard, a calibration curve (y=-2.2078x + 0.6508) was prepared using trolox (6-hydroxy-2,5,7,8-tetra-methylchroman-2-carboxylic acid) and the results were obtained. In the 10 μl trolox solution diluted in the assay buffer, 20 μl myoglobin working solution (100-fold with 1x assay buffer) and 150 μl ABTS substrate working solution were mixed. After incubation at room temperature for 5 minutes, 100 μl stop solution was mixed and measured at 734 nm with a micro plate reader (SpectraMax M2e, Molecular Devices, USA). The scavenging activity was expressed as trolox equivalents (trolox equivalent antioxidant capacity, TEAC) in the trolox standard curve. As 1x assay buffer, 10x assay buffer (A3605, Sigma-Aldrich, USA) was diluted 10-fold with distilled water, and ABTS substrate working solution was mixed with 1 ABTS tablet, 1 phosphate-citrate buffer tablet and 100 ml of distilled water.

Parallel artificial membrane permeability assay

Transmittance analysis was performed using a Pampa Evolution Instrument (Pion Inc. USA). Each sample was extracted and used as a stock solution, and 100 μl of stock solution was dispensed into a stock plate. Prisma HT buffer (110151, Pion Inc., MA, USA) was diluted 40 times to prepare Prisma Buffer prepared at pH 7.4. After 1000ul of prisma buffer was added to the deep well plate, 200ul of sample from the stock plate was taken and transferred to a deep well plate, and then mixed 4-6 times with a pipette. For the GIT assay, 180 µl of the sample solution was transferred from the deep well plate to the donor plate. After completely dissolving GIT-0 lipid (1ml/vial), the lid of the Acceptor plate was removed, and 5µl of lipid was directly wetted into the membrane. As for the acceptor plate, 200 µl of ASB solution (Acceptor Sink Buffer, Pion Inc, Billerica, USA) was added to each well. Taking care not to create a bubble, put it on the donor plate from the bottom row of the acceptor plate to make a sandwich plate, and then put the lid on the acceptor plate. It was incubated for 1 hour in Gut-Box™ (Pion Inc., Billerica, USA) under the condition of 40 ABL (Aqueous Boundary Layer Thickness). After incubation, the samples remaining on the acceptor plate and the donor plate were placed in 2ml e-tubes, respectively, and analyzed by LC/MS (Shimadzu UPLC-MS). Phenomenex Luna 1.6u C18(2) 100A 150 x 2.10 mm was used as the column, and the Intection volume was set to 3 µl and a flow rate of 0.3 mL/min. A PDA detector was used for the detection UV and Water (0.1% of formic acid in water)-Acetonitrile Gradient elution was used for the mobile phase.

CMGT powdering of turmeric, ginger and aronia

Turmeric, ginger and aronia were purchased and washed thoroughly under running water to prepare. The prepared turmeric, ginger and aronia were immersed in liquid nitrogen, frozen at -190°C, and supplied while pulverizing liquid nitrogen, and ultra-low temperature pulverization was performed while maintaining -190°C. At this time, the pulverized turmeric, ginger and aronia had a particle size of 10-50 μm and a particle temperature of -120 to -20°C.

On the other hand, turmeric, ginger, and aronia were pulverized by a general pulverization method in order to compare with the cryogenic ultra-fine pulverization according to the present invention. For general pulverization, dry raw materials were pulverized for 9 minutes with a pulverizer (DHM-7000CW, Daesung Artlon, Paju, Korea) and passed through an 80 mesh network as a sample.

Comparison of CMGT-mixed particle size and disintegrated particle size of CMGT-granules

The particle size of each sample (CMGT-mixed and CMGT-granule) was analyzed through a wet particle size analyzer, and the particle size distribution 10% and 90% values, and the average particle diameter and median values were measured by repeating measurements three times. It is shown in the table.

FBG (fluid) at low temperature (25 to 35°C) using a sample of raw material having an average particle size of turmeric 17.7 μm, aronia 14.7 μm, and ginger 19.9 μm of mixing (CMGT-mixed) of the first fine powder and the second fine powder bed granulation) process was carried out to prepare CMGT-granules.

When the CMGT-granules of each sample thus prepared were ingested into the body again, the disintegration particle size was checked to determine the size of the particle size used in the body. The disintegration particle size was 16.3 μm of turmeric, 16.6 μm of aronia, and 20.6 μm of ginger.

Sample particle size analyzer measurement result sample Particle diameter (㎛) Mean Median d10 d90 Turmeric
CMGT mix 17.765±0.938 18.759±0.646 6.164±1.071 47.161±1.530 Granules 16.366±0.225 17.865±0.009 5.215±0.033 43.512±0.213 Aaronia
CMGT mix 14.703±0.486 15.985±0.470 5.287±0.119 34.238±1.709 Granules 16.677±0.363 18.364±0.354 5.312±0.140 43.012±0.667 ginger
CMGT mix 19.961±0.465 19.899±0.271 5.864±0.080 69.826±3.712 Granules 20.670±0.119 20.460±0.112 7.194±0.110 63.003±0.761

The average particle size of CMGT-mixed turmeric was 17.765㎛, and the average disintegrating particle size of CMGT-granules (FBG) was 16.696㎛, and the average particle size of CMGT-granules was 1.069㎛ smaller than that of CMGT-mixed. Confirmed. At 10% and 90% of particle size distribution, CMGT-mixed showed a particle size distribution of 6.164㎛ and 47.161㎛, and CMGT-granule showed a particle size distribution of 5.408㎛ and 45.817㎛. Through this, it was found that there was no significant difference between the CMGT-mixed and CMGT-granules of turmeric.

The average particle size of Aaronia's CMGT-mixed 3 times was 14.703㎛, and the average disintegrating particle size of CMGT-granules was 14.440㎛. The average particle size of CMGT-granules and the average particle size of CMGT-mixed were not significantly different. Did.

The average particle size of CMGT-mixed ginger was 19.961㎛, and the average disintegration particle size of CMGT-granules was 21.437㎛, and the average particle size of CMGT-granules was 1.476㎛ smaller than that of CMGT-mixed. At 10% and 90% of particle size distribution, CMGT-mixed showed a particle size distribution of 5.864㎛ and 69.826㎛, and CMGT-granule showed a particle size distribution of 6.104㎛ and 76.473㎛. Through this, it was found that there was no significant difference between the CMGT-mixed and CMGT-granules of ginger.

From this, it was found that while taking advantage of the CMGT technology, which is a cryogenic ultrafine grinding method, the dispersibility can be improved through the FBG technology, and after ingestion in the body, it disintegrates and acts as ultrafine particles again.

Comparison of chromaticity of CMGT-mixed and CMGT-granules

The chromaticity of the sample was investigated using a colorimeter (ColorQuest XE, HunterLab, USA) for the brightness L (lightness), redness a (redness), and yellowness b (yellowness), and the standard white plate used at this time. )'S L value is 96.60, a value is 0.24, and b value is 1.97. Table 2 shows the results of chromaticity analysis of each sample through a colorimeter.

Sample colorimeter measurement result sample Colorimeter data L a b Turmeric
CMGT mix 71.27±0.006 7.01±0.020 67.80±0.098 Granules 70.78±0.032 7.30±0.040 68.29±0.424 Aaronia
CMGT mix 36.12±0.115 19.91±0.062 6.15±0.144 Granules 34.52±0.084 20.03±0.091 6.08±0.288 ginger
CMGT mix 78.58±0.035 -0.50±0.036 26.45±0.108 Granules 78.31±0.031 -0.24±0.042 27.46±0.104

Considering that the antioxidant component is mainly a pigment component, the change of the antioxidant component can be predicted sensoryly through the change in chromaticity according to CMGT-mixing and CMGT-granules. As a result of chromaticity analysis, there was a tendency that the brightness slightly decreased after passing through the FBG process, but it was confirmed that the color difference between CMGT-mixed and CMGT-granules was not large. From this, it was confirmed that the FBG process did not exhibit large discoloration due to heat and was excellent in preservability of the dye component.

On the other hand, it was confirmed that when ultra-fine pulverization at cryogenic temperatures was performed rather than normal pulverization, the brightness increased and tended to become brighter. This means that there is a possibility that the color of the sample may change due to heat generated when pulverization is performed at room temperature, resulting in a decrease in brightness.When ultra-fine pulverization at cryogenic temperatures is carried out, the original color of the sample is maintained and the colorimeter is fined. It was estimated that the brightness was higher than that of the general pulverized material when measured by.

Comparison of effect of general powder and CMGT powder

Change in total polyphenol content

In general, polyphenol compounds are known to have blood pressure prevention, gout and whitening effects. One of the most noticeable things for the physiological activity of polyphenols is that they donate hydrogen atoms to free radicals as their antioxidant activity, thereby inhibiting the production of free radicals such as free radicals, which are excessively generated by oxidative stress in the living body, thereby exhibiting antioxidant activity. It is known to do. The used gallic acid standard curve is shown in FIG. 4. The calibration curve showed good linearity (R2=0.9954).

In order to measure the total polyphenol content of the general pulverized powder of turmeric, ginger and aronia and CMGT powder samples, the concentration of each sample was diluted to fall within the measurement range of the calibration curve, and the absorbance was measured. Same as CMGT powder samples of turmeric, ginger, and aronia showed significantly higher total polyphenol content than those of normal ground powder samples. In the case of CMGT powder of turmeric, the total polyphenol content was 0.104mg/g, which was 1.35 times higher than that of the general pulverized turmeric product of 0.077. Ginger CMGT powder had a total polyphenol content of 0.143, which was 1.4 times higher than that of general ginger powder of 0.101. In the case of Aaronia CMGT powder, the total polyphenol content was 0.779, which was 1.16 times higher than the total polyphenol content of Aaronia general pulverized product, 0.669. From this, it was confirmed that the CMGT powdering technique for turmeric, ginger and aronia was an advantageous processing method for maintaining the polyphenol component of the sample compared to the general grinding technique.

Total polyphenol content in each sample Total polyphenol content
(mg·GAE/g) Normal CMGT powder Turmeric 0.077±0.0008 0.104±0.0016 ginger 0.101±0.0002 0.143±0.0008 Aaronia 0.669±0.0045 0.779±0.0012

ABTS radical scavenging activity change

Polyphenol compounds have been studied a lot as natural antioxidants that remove active oxygen generated in the body due to their structural characteristics. In order to evaluate the antioxidant activity of turmeric, ginger, and aronia, ABTS radical scavenging efficacy was measured and the correlation thereof was confirmed, and the used Trolox standard curve is shown in FIG. 5. The calibration curve showed good linearity (R2=0.996).

In order to measure the ABTS radical scavenging ability for the general pulverized powder of turmeric, ginger and aronia and CMGT powder samples, each sample was diluted in three concentrations so as to fall within the measurement range of the calibration curve. same. As a result, it was confirmed that the CMGT powder samples of turmeric, ginger, and aronia were measured significantly higher in antioxidant activity in the same trend as the result of the total polyphenol content, which confirmed the same result as DPPH radical scavenging activity. From this, it can be seen that the CMGT powdering technology is an advantageous processing method for maintaining the antioxidant capacity of the sample compared to the general drying and general grinding technology.

ABTS radical scavenging ability of each sample Trolox (mg/ml) Normal CMGT powder Turmeric 1.63±0.041 2.17±0.027 ginger 2.38±0.162 3.00±0.152 Aaronia 32.69±1.880 36.45±0.250

Artificial lipid membrane permeation analysis

The goal of this experiment is to check the membrane permeability of an indicator material using the PAMPA (Parallel Artificial Membrane Permeability Assay) system, an artificial lipid membrane. The artificial lipid membrane PAMPA system is an in vitro model for viewing the permeability of substances that passively penetrate cell membranes. That is, it is possible to measure only the transmittance of a material in the case of diffusion using the PAMPA system. When a material having a high molecular weight passes through the membrane, the transmittance of the water-soluble material is significantly lower than that of the oil-soluble material. Due to the properties of these materials, it was possible to confirm the permeability between the indicator materials. The transmittance measurement results are shown in Table 5.

The transmittance of turmeric CMGT was found to be about 16% higher than that of the general pulverized material, and the transmittance of Aaronia CMGT was found to be about 30% higher than that of the general pulverized material. In the case of ginger, the transmittance of the general pulverized product and CMGT was similar, but the permeated concentration was higher in CMGT, and the results are shown in Table 6.

Comparison of transmittance of each sample Transmittance (%) Normal CMGT powder Turmeric 21.91 37.44 ginger 36.28 35.47 Aaronia 28.37 59.27

Ginger transmittance comparison ginger
6-gingerol Normal CMGT powder HT Acceptor HT Acceptor Peak area 192.591 69.88 324.963 115.273 % 100 36.28 100 35.47

At this time, the ratio of donor and acceptor was measured after the experiment based on 100% of extract + HT as a sample before entering the gut box. The transmittance was defined as a% value obtained by dividing the peak area value identified by the acceptor by the peak area value of the extract + HT.

When the 6-gingerol concentration in the ginger extract was confirmed as the peak area of the HT sample, the peak area of CMGT was 324.963, and the normal pulverization was 192.591, which was about 1.7 times higher in CMGT. In addition, the concentration of permeable index component 6-gingerol was detected 1.6 times higher in CMGT and 69.88 in general pulverized material.

CMGT-mixing and preparation of CMGT-granules

In the case of CMGT-mixed and CMGT-granules, it was confirmed that they were well maintained without destruction of antioxidant components. The moisture content and dispersibility were checked, and the first fine powder and the second mixing ratio according to the present invention were specified. The content of aronia, ginger, and turmeric (second fine powder) in a state containing moisture after CMGT 2 was set to 2 to 30% by weight, and the degree of dispersion according to the degree of mixing was confirmed. At this time, if the amount is less than 2% by weight, the dispersion index may be high, but it was confirmed that the effect of shortening the operating time during the operation of FBG does not occur because the water content retained in the overall contents does not provide much nutrition. The (wet) content of the powder was set to 2% or more. In addition, when 25% by weight or more is added, the content has the effect of lowering the overall temperature by increasing the retained moisture content of the content. . From this, the total moisture content of the mixture of the first fine powder and the second fine powder was calculated as 5 to 20% by weight. The content ratio of the first fine powder and the second fine powder to which this was applied is shown in Table 7.

division Mixing ratio (CMGT1+CMGT2) 2% 5% 10% 15% 20% 25% 30% Aaronia CMGT2 2.00 5.00 8.00 15.00 21.00 28.00 35.00 CMGT1 98.00 95.00 92.00 85.00 79.00 72.00 65.00 ginger CMGT2 2.00 4.50 8.00 13.00 19.00 25.00 30.00 CMGT1 98.00 95.50 92.00 87.00 81.00 75.00 70.00 Turmeric CMGT2 2.00 3.00 7.00 13.00 20.00 26.00 32.00 CMGT1 98.00 97.00 93.00 87.00 80.00 74.00 68.00

Changes in total polyphenol content and ABTS radical scavenging activity

Changes in total polyphenol content, antioxidant activity, and transmittance of CMGT-mixed and granulated CMGT-granules obtained by mixing the first fine powder and the second fine powder were examined. As a result, CMGT-granules were slightly lower overall than that of CMGT-mixed, but when looking at the maintenance of sulfation activity, it was confirmed that there was no significant difference in the antioxidant activity of turmeric, ginger, and aronia. This was predicted to be due to the fact that polyphenols were substituted with other substances by the FBG process or the total antioxidant active substances in the sample were maintained by the antioxidant active substances generated during the FBG process. From this, it can be seen that the FBG process is a technology capable of granulating while maintaining the antioxidant activity of the sample.

Changes in total polyphenol content of CMGT-mixed and CMGT-granules Total polyphenol content
(mg·GAE/g) mix Granules Turmeric 0.096±0.0009 0.094±0.0014 ginger 0.124±0.0013 0.116±0.0004 Aaronia 0.284±0.0015 0.241±0.0011

Changes in ABTS radical scavenging activity of CMGT-mixed and CMGT-granules Trolox (mg/ml) mix Granules Turmeric 1.90±0.049 1.93±0.071 ginger 2.49±0.136 2.71±0.106 Aaronia 1.43±0.060 1.29±0.033

Artificial lipid membrane permeation analysis

When comparing the permeability of the CMGT-mixed and the CMGT-granules to the artificial lipid membrane, the transmittance of the CMGT-granules was almost similar or higher than that of the CMGT-mixed. When the effect of CMGT-FBG processing technology conditions on the absorption rate of nutrients of turmeric, ginger and aronia in vivo was confirmed, first, the basic transmittance was increased with the CMGT processing technology (CMGT grinding has a transmittance compared to general grinding processing). In addition, it was confirmed that the index component is not destroyed when granules are formed due to the FBG process technology of this patent and can maintain extraction efficiency and transmittance.

Comparison of transmittance of CMGT-mixed and CMGT-granules Turmeric mix Granules PAMPA transmittance (%) 7.60% 8.30% ginger mix Granules PAMPA transmittance (%) 16.00% 16.60% Aaronia mix Granules PAMPA transmittance (%) 9.00% 11.30%

CMGT-granular dispersion

In order to improve the dispersion degree of CMGT-granules numerically, the degree of dispersion of the dextrin standard was calculated as 10, and those with little dispersion degree were calculated as 1 and scored. The content of the second fine powder (raw material) was mixed and applied at 2 to 30% by weight, and the moisture content of the first fine powder and the second fine powder mixture was calculated at a level of 5 to 20% by weight, and the content of the second fine powder was refined. The degree of dispersion according to the was quantified. The degree of dispersion according to the raw material content itself is high dispersion of the raw material content of 2 to 4%, but if the dispersion degree is scored in consideration of the workability of the whole mixture in the FBG environment, the dispersion work of the raw material content of 5 to 15% by weight I could find that the castle was better. From this, it was found that the content of the effective raw material was 2 to 20% by weight, and more preferably, it was found that the content of the effective raw material was 7 to 15% by weight when considering the dispersion degree and the appropriate moisture content as a whole.

division Raw material content
CMGT2(wet) 2% 7% 10% 15% 20% 25% 30% FBG application
Dispersion
score Aaronia 4 5 6 5 3 2 One ginger 5 7 7 7 4 3 One Turmeric 5 7 7 6 4 3 One

Claims (12)

(a) preparing an antioxidant plant consisting of turmeric, ginger and aronia;
(b) the antioxidant plant selected from the group consisting of turmeric, ginger and aronia was frozen at -196°C to -70°C and pulverized to a size having an average particle size of 10 μm to 50 μm, and the pulverized product was 10°C to 30 Drying at °C to prepare a first fine powder;
(c) The antioxidant plant selected from the group consisting of turmeric, ginger and aronia prepared in step (a) is frozen at -196°C to -70°C and pulverized to a size having an average particle size of 10 μm to 50 μm to contain moisture. Producing a second fine powder, characterized in that;
(d) mixing the first fine powder and the second fine powder in an airflow manner in a zero gravity state; And
In the method for producing antioxidant plant granules comprising the step of (e) granulating the mixture at 25 ℃ to 35 ℃,
The first fine powder is characterized in that 85 to 93% by weight and the second fine powder is 7 to 15% by weight,
The method for producing antioxidant plant granules, characterized in that the mixture of the first fine powder and the second fine powder contains 5 to 20% by weight of moisture.
The method of claim 1,
The antioxidant plant granules method for producing antioxidant plant granules, characterized in that maintaining the polyphenol content of the raw material.
The method of claim 1,
The method for producing antioxidant plant granules, characterized in that the antioxidant plant granules have antioxidant activity.
The method of claim 1,
The antioxidant plant granule is a method of producing an antioxidant plant granules, characterized in that the absorption rate in the body increases.
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