KR20130116396A - Photoreactor with external light source - Google Patents

Photoreactor with external light source Download PDF

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Publication number
KR20130116396A
KR20130116396A KR1020120023245A KR20120023245A KR20130116396A KR 20130116396 A KR20130116396 A KR 20130116396A KR 1020120023245 A KR1020120023245 A KR 1020120023245A KR 20120023245 A KR20120023245 A KR 20120023245A KR 20130116396 A KR20130116396 A KR 20130116396A
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KR
South Korea
Prior art keywords
incubator
light source
photoreactor
external light
flow path
Prior art date
Application number
KR1020120023245A
Other languages
Korean (ko)
Inventor
정국
정재훈
김봉조
이수영
남지현
임준혁
이제근
이태윤
유영문
Original Assignee
부경대학교 산학협력단
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Publication date
Application filed by 부경대학교 산학협력단 filed Critical 부경대학교 산학협력단
Priority to KR1020120023245A priority Critical patent/KR20130116396A/en
Publication of KR20130116396A publication Critical patent/KR20130116396A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor

Abstract

PURPOSE: A photoreator with an external light source is provided to actively supply light and gas to microalgae and to culture microalgae using the light and the gas. CONSTITUTION: A photoreactor with an external light comprises: a flow path (14) with a tubular vertical section; a culture machine (12) which is connected to one or more flow paths; and a light emitting element (15) which is mounted at the outside of the culture machine and irradiates the flow paths by light. The culture machine and the flow paths are made of a light transmitting material. In the culture machine, one or more flow paths are piled and inclined. Each end of the flow paths is connected to another one.

Description

Photoreactor with external light source {PHOTOREACTOR WITH EXTERNAL LIGHT SOURCE}

The present invention relates to a photoreactor, and more particularly, to a photoreactor having an external light source for culturing microalgae by supplying light and gas from the outside.

Microalgae is a general term for photosynthetic single-celled organisms, and is composed of various types of photosynthetic organisms that can produce biomass more efficiently and faster than land plants. Some of these species can produce more than 50% of their biomass as fat, and many of these fats can be triglycerides, a source of biodiesel, which is high in energy-dense biodiesel and green aircraft. Expectations are being made for materials such as fuel and green gasoline. Microalgae feed on sunlight, medium and carbon dioxide, and can control the environment to grow much faster in the photobioreactor.

A reactor that decomposes organic compounds by causing a photocatalytic oxidative decomposition reaction by mixing a predetermined amount of a photocatalytic material in wastewater containing organic compounds in Korean Patent Publication No. 10-2010-0136602 using conventional reactor technology. A non-uniform PFR (Plug Flow Reactor) type photocatalytic reactor for the decomposition of organic compounds contained in wastewater is disclosed.

However, the conventional reactor technology has a problem that the supply of light and gas is passive.

Republic of Korea Patent Publication No. 10-2010-0136602 (2010.12.29.)

An object of the present invention for solving the above problems is to provide a photoreactor having an external light source for actively supplying light and gas required for microalgae cultivation and the light and gas supplied to the microalgae cultivation efficiently.

In order to achieve the above object, the present invention is a light having a light source having an external light source including a light emitter for irradiating light through the incubator and the incubator formed by connecting a plurality of passages and a tubular channel having a vertical cross section is a circle or polygon. Provide a reactor.

In addition, the incubator and the flow path may be a light transmissive material.

In addition, the incubator may be formed by stacking a plurality of flow paths inclined and both ends are alternately connected.

In addition, the light emitter may be one or more LED assemblies that can be inserted into a gap formed by connecting a flow path.

In addition, the lower tank connected to the incubator lower tube and the upper tank connected to the incubator upper tube and one end is connected to the lower tank and the pipe, the other end may further include a pump connected to the upper tank and the pipe.

In addition, the culture medium may be transferred from the lower tank to the upper tank using a pump and introduced into the incubator by free fall.

In addition, it may further include an injector for supplying gas to the bottom of the incubator.

In addition, the gas supplied to the bottom of the incubator may flow in the flow path.

According to the photoreactor having the external light source of the present invention described above, light and gas are actively supplied to the microalgae, the light is continuously used for microalgae cultivation and the gas is actively used for microalgae cultivation without stagnant in the incubator It is effective.

1 is a schematic diagram of an optical reactor having an external light source, which is one embodiment of the present invention;
Figure 2a is a photograph of the incubator of the photoreactor having an external light source of Figure 1,
FIG. 2B is a schematic diagram of a modification of the incubator of the photoreactor having the external light source of FIG. 1; FIG.
FIG. 2C is a schematic diagram of another modification of the incubator of the photoreactor having the external light source of FIG. 1; FIG.
FIG. 2D is a schematic view of the incubator of the photoreactor having the external light source of FIG.
3 is a photograph of a light emitting body to be attached to the outside of the photoreactor having the external light source of FIG.
4 is a photograph in which the light emitter of FIG. 3 is inserted into a gap to emit light;
5 is a graph of tetraselmis growth according to the emission color of the LED light source,
6 is a graph of tetraselmis growth according to the emission color of the LED light source and the presence or absence of gas supply.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

1 is a schematic diagram of a photoreactor having an external light source, which is one embodiment of the present invention.

As shown in the figure, a tube-shaped flow path 14 having a vertical cross section and a plurality of flow paths 14 connected to the incubator 12 and the incubator 12 formed by connecting a plurality of light emitters are irradiated with light to the flow path 14. It consists of 15 pieces.

The tubular flow path 14 having a vertical cross section is formed in a wide width and a low height by combining a horizontal partition member 13 and a vertical side member 16. The incubator 12 includes a plurality of flow paths. 14 is inclined and stacked, and both ends are alternately connected. The incubator 12 and the flow passage 14 are light transmissive materials.

The incubator 12 is described in detail as follows. The adjacent partition member 13 of one end of the inclinedly stacked flow path 14 is directly abutted to form a predetermined angle, and the remaining partition member 13 and the side member 16 extend to seal the incubator 12. Is connected. The plurality of flow paths 14 are alternately connected at both ends in the same manner to form a zigzag incubator 12. The upper and lower partition members 13 forming a flow path 14 positioned at the top and bottom of the incubator 12 are horizontal to the ground.

A medium that is mixed with a medium and a microalgae and suspended in seawater is defined as a culture solution.

Due to the shape of the incubator 12, the length of time that the culture solution stays in the incubator 12 becomes long. A gap is formed between the flow paths 14 connected to form the incubator 12. The light emitting body 15 is inserted into the gap to irradiate light to the flow passage 14 from the outside of the incubator 12. The light emitter 15 is one or more LED assemblies that can be inserted into a gap. In the photoreactor having an external light source of one embodiment of the present invention, a plate-shaped LED is used as the light emitter 15. The plate-shaped LED emitter 15 is connected to a power supply 18 and an electric wire 17 located outside to supply power.

Photoreactor having an external light source of an embodiment of the present invention and the lower tank 23 and the upper tank 10 is connected to the upper tube 11 and the incubator 12 and the lower tube incubator 12 and One end is connected to the lower tank 23 and the pipe 25, and the other end further includes a pump 26 connected to the upper tank 10 and the pipe 27.

The lower tank 23, the upper tank 10, the incubator 12, the lower tank 23 is configured in the circulation system to move the culture fluid.

The culture solution is introduced into the lower tank 23 through the inlet 24. In addition, the culture medium is recovered through the discharge valve 22 formed on the lower water tank 23 side.

The upper tank 10 and the lower tank 23 which are positioned up and down are connected to the pipes 25 and 27, and the culture medium is fed from the lower tank 23 to the upper tank along the pipes 25 and 27 by the force of the pump 26. (Function of 25) The culture medium moved to the upper tank 10 flows through the tube 11 into the incubator 12 located below the upper tank 10 by free fall and incubator 12. Flow from top to bottom. Passing through the incubator 12, the culture fluid is moved to the lower tank 23 through the tube 19, and is moved back to the upper tank 10 by the force of the pump 26.

In addition, the photoreactor having an external light source of one embodiment of the present invention further comprises an injector 21, the tube 20 is inserted into the lower end of the incubator 12 to supply gas, the air is injector 21 It is supplied to the lower end of the incubator 12 to flow in the flow path 14 in a bubble state.

The light irradiated from the light emitter 15 and the gas supplied from the injector 21 are actively supplied into the incubator 12, thereby increasing the microalgal culture efficiency.

2A is a photograph of the incubator of the photoreactor having the external light source of FIG. 1, in which a flow path 14 formed by combining the partition member 13 and the side member 16 is inclined and alternately connected at both ends thereof to incubator 12. ) Is formed.

Unlike the above, when the flow path 14 is stacked flat, and both ends of the flow path 14 are alternately connected to form the incubator 12, the culture solution is stagnated near the connection portion of the flow path 14 to lower the culture efficiency. do. (See FIG. 2B).

When the inclined flow path is formed by stacking a plurality of partition members 13 alternately as shown in FIG. 2C, bubbles A flow smoothly from the bottom of the incubator 12 to the top, but the culture medium flows from the top of the incubator 12 to the bottom. The flow (F ') of the stagnant section (B) is generated, the culture efficiency is lowered because the bubble (A) and the culture medium is not mixed in the stagnant section (B) (see Fig. 2c).

2D shows that both ends of the partition member 13 are connected in a zigzag to form a flow path 14, and the flow paths 14 are inclined and stacked to form an incubator 12. The flow (F) of the bubble (A) flowing from the bottom of the incubator 12 to the top is naturally continued, and at the same time, the culture efficiency by eliminating the congestion section (B) of the corner generated in the flow (F ') of the culture fluid flowing from the top to the bottom. Can be increased (see FIG. 2D).

FIG. 3 is a photograph of a light emitting body to be attached to the outside of the photoreactor having the external light source of FIG. 1, and FIG. 4 is a photograph of the light emitting body of FIG. 3 inserted into a gap to emit light.

As shown, the light emitter 15 was made of a plate-shaped LED. In the photoreactor having an external light source of one embodiment of the present invention, the LED 15 is attached to a gap formed by the passage 14 connected thereto. It is located outside the incubator 12, but because it emits light in close proximity to the flow path 14, it is possible to irradiate light evenly to the flow path (14).

5 is a graph of tetraselmis growth according to the light emission color of the LED light source, and FIG. 6 is a graph of tetraselmis growth according to the emission color and gas supply of the LED light source.

Using a photoreactor having an external light source of an embodiment of the present invention described above incubated tetracelmis in the medium of Table 1 and the conditions of Table 2 below to obtain the graph of Figure 5 and the graph of Figure 6.

badge additive reagent



f / 2 badge

f / 2 trace metal solution


FeCl 3 .6H 2 O, Na 2 EDTA.2H 2 O,
CuSO 4 .5H 2 O, Na 2 MoO 4 .2H 2 O,
ZnSO 4 7H 2 O, CoCl 2 7H 2 O,
MnCl 2 4H 2 O,
Vitamin B 2 (cyanocobalamin),
Biotin, Thiamine HCl


f / 2 vitamin solution

variable Condition reagent
LED

Red light; White light; 25 days each

Sea water, H 2 O, NaNO 3 ,
NaH 2 PO 4 H 2 O, Na 2 SiO 3 9H 2 O,
f / 2 trace metal solution,
f / 2 vitamin solution
LED, injector

Red, on; Red, off; White light, on;
White light, off; 15 days incubation each

Tetraselmis is a photosynthetic pigment with chlorophylls a and b . It produces mannitol in the early photosynthetic products, and has four isotonic flagellums, which are single-celled or colony green algae whose main components are galactose and uronic acid. .

The light from the plate-shaped LED illuminant 15 is divided into a case of red light and a case of white light, and the mixed solution incubated for 25 days is repeated 6 times at intervals of a hemacytometer in a 9-time interval of tetracells in each cell ( The result of measuring the number of Tetraselmis) with an optical microscope is the graph of FIG.

In the case of white light, the graph is steeper and the y-axis displacement is large. Therefore, it can be inferred that tetraselmis grows better in white light that emits complex light than red light in most conditions.

In addition, the light supplied from the plate-shaped LED light emitter 15 is divided into the case of red light and white light when the injector 21 is turned on and off when incubated for 15 days in tetracell mist under a total of four conditions Figure 6 The graph's results were obtained.

When the same light was supplied, the gas supplied side showed a very high initial culture rate. This can be interpreted that the influx of carbon source due to the gas supply has a great influence on the culture of tetraselmis.

If there is some medium in the growth of microalgae, the presence or absence of carbon source greatly influences the culture, and the change according to the wavelength of light supplied has the next influence.

The embodiments of the present invention described in the present specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention and are not intended to cover all the technical ideas of the present invention. Therefore, various equivalents And variations are possible.

Accordingly, the present invention is not limited to the above-described embodiments, and various modifications can be made by those skilled in the art without departing from the gist of the present invention as claimed in the claims. Such changes will fall within the scope of the claims.

10: upper tank 11, 19, 20, 25, 27: tube 12: incubator
13: partition member 14: flow path 15: luminous body
16: side member 17: wire 18: power supply
21: injector 22: discharge valve 23: lower water tank
24: Inlet 26: Pump A: Bubble
B: Stagnation section F: Bubble flow F ': Culture medium flow

Claims (8)

Vertical section is tubular flow path; and
An incubator formed by connecting one or more of the flow paths; and
A photoreactor having an external light source installed outside the incubator and including a light emitter for irradiating light to the flow path. The method of claim 1,
The incubator and the flow path is an optical reactor having an external light source, characterized in that the light transmitting material. The method of claim 1,
In the incubator, the flow path is inclined, one or more are stacked, one end of each flow path is an optical reactor having an external light source, characterized in that formed. The method of claim 3,
The light emitter is a photoreactor having an external light source, characterized in that one or more LED assembly that can be inserted into the space outside the incubator formed by the passage is connected. The method of claim 1,
A lower water tank connected to the lower portion of the incubator;
An upper water tank connected to the upper portion of the incubator; and
One end is connected to the lower tank and the pipe, the other end is connected to the pipe and the upper tank; Photoreactor having an external light source further comprises. The method of claim 5,
The photoreactor having an external light source, characterized in that the culture medium is moved from the lower tank to the upper tank using the pump and introduced into the incubator by free fall. The method of claim 1,
Photoreactor having an external light source, characterized in that it further comprises an injector for supplying gas to the bottom of the incubator. 8. The method according to claim 3 or 7,
And a gas supplied to the lower end of the incubator flows along the flow path.
KR1020120023245A 2012-03-07 2012-03-07 Photoreactor with external light source KR20130116396A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114367253A (en) * 2022-01-17 2022-04-19 万华化学集团股份有限公司 Optical channel reactor and preparation method of high-purity all-trans vitamin A acetate and derivative crystal thereof
WO2022170371A1 (en) * 2021-02-03 2022-08-11 Peter Douglas Apparatus for organism rearing or liquid remediation

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022170371A1 (en) * 2021-02-03 2022-08-11 Peter Douglas Apparatus for organism rearing or liquid remediation
CN114367253A (en) * 2022-01-17 2022-04-19 万华化学集团股份有限公司 Optical channel reactor and preparation method of high-purity all-trans vitamin A acetate and derivative crystal thereof
CN114367253B (en) * 2022-01-17 2024-04-09 万华化学集团股份有限公司 Optical channel reactor and preparation method of high-purity all-trans vitamin A acetate and derivative crystal thereof

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