TWI440429B - A photo bio-reacting system - Google Patents

Description

Photobiochemical reaction system

The present invention relates to a photobiochemical reaction system, and more particularly to a photobiochemical reaction system for cultivating plant cells or animal cells.

The research atmosphere of algae cultivation has prospered in recent years, but the biggest problem in cultivating algae is that the light-receiving area of the reactor is limited, resulting in low yield and low land use efficiency. Therefore, how to develop a new type of photoreactor and improve the culture efficiency is yet to be further studied.

The development of microalgae is currently limited by the cost of cultivation. Open reactors require large amounts of land, while closed reactors require expensive facilities and culture techniques, so only a few commercial practices are based on open reactors. The biggest disadvantage of the traditional open pond culture is that when the depth of the culture pond is deep, the light can not reach the bottom end of the culture tank, and the stirring cannot be sufficiently performed, so that the efficiency of the culture is lowered. The new photobiochemical reaction system overcomes the shortcomings of the traditional open reactor, increases the illumination area per unit area and the gas-liquid exchange contact area, more effectively improves the light utilization efficiency, and reduces the cultivation cost to produce production in advance.

The prior art patent 093215067 discloses a plant-based algae and microbial photosynthetic reactor for cyclically performing photosynthesis and venting of a culture liquid of plant algae and microorganisms injected therein; the plant algae and microbial photosynthetic The reactor comprises a photosynthetic reaction pipeline, a pressurized infusion module and an exhaust and adjustment module; the photosynthetic reaction pipeline is a light transmission pipeline; the exhaust and regulation module is an exhaust cylinder, and The liquid cylinder and an adjusting cylinder are assembled to facilitate manufacture and assembly, and the effect of multiple oxygen discharges is formed, so that the oxygen generated in the culture liquid is easily discharged quickly. Only in the above patents, the photosynthetic reactors are arranged in a flat shape, which cannot be stacked and thus has a small scale.

The prior art 093136923 patent discloses a microbial culture device which uses a connecting tube to connect a plurality of small culture tanks to each other so that liquid substances in each culture tank can be interchanged with each other by the communication tube, thereby achieving a large number of cultures. The purpose is not to bear the cost of making large culture tanks. It is only in the above patent case that the culture device cannot be stacked because of the flatness and fails to increase the light efficiency and its scale is limited in a limited space.

The prior art 098205395 patent discloses a spirulina stereotactic photosynthetic reactor comprising: an ultrafilter; a reverse osmosis seawater desiccator; a nutrient replenishing tank; a flue gas seawater desulfurization tank; and a plurality of spirulina cultures The troughs can be stacked in a three-dimensional manner; a spirulina hydrodynamic rotary separator; a spirulina filtration separation tank; and a centrifugal separator. By adopting the above-mentioned structure to support the stereoscopic photosynthetic reactor, carbon dioxide in the flue gas generated in the power generation place can be used as a carbon source for growth, and oxygen can be released by photosynthesis, in addition to growing spirulina, It can reduce the greenhouse effect caused by carbon dioxide emissions from flue gas. In the above patents, the photosynthetic reactors are arranged in a stereoscopic plane, which has limited light-receiving efficiency and thus cannot produce better efficiency and productivity.

It can be seen that although the photosynthetic biological culture system can be cultured in a large amount, the efficiency of light utilization is still limited, and the production cannot be greatly improved, and the cost of the culture medium cannot be reduced. This is not a good design and needs to be improved.

Therefore, there is a need for a photosynthetic biological culture system (i.e., photobiochemical reaction system) that has a high degree of light utilization efficiency to increase the yield per unit area.

An example of the present invention provides a photobiochemical reaction system comprising: a plurality of photosynthetic culture modules, each of the photosynthetic culture modules having a top, a bottom, and a bottom and a bottom In the first part, the first portion has an inner diameter smaller than the top portion and the bottom portion, and each of the photosynthetic culture modules has a light transmissive property; and a light emitting module that emits light of a specific wavelength. The light of the particular wavelength illuminates and penetrates each of the permeable, photosynthetic breeding modules.

Other features and advantages of the invention will be set forth in part in the description in the description. The features and advantages of the present invention will be understood and attained by the <RTIgt;

The above summary, as well as the following detailed description, are for the purpose of illustration and explanation,

Reference will now be made in detail to the embodiments of the invention, Wherever possible, the same reference numerals will be used to refer to the

1 is a diagram of a photobiochemical reaction system 10 in accordance with an embodiment of the present invention. Referring to FIG. 1 , the photobiochemical reaction system 10 can include at least one layer of a plurality of photosynthetic breeding modules 11 , a nutrient supply module 12 , a gas supply module 13 , a collection module 14 , and a gas detection module 15 . And a light emitting module 16.

The light-emitting module 16 extends along an axial direction, and the at least one layer of the photo-culture module 11 surrounds the light-emitting module 16 and is arranged along the radial direction. In an embodiment of the present invention, the photobiochemical reaction system 10 further includes a plurality of photosynthetic breeding modules 11 that are arranged in a plurality of layers around the light emitting module 16 and arranged in a radial direction. Group 11 can be stacked along the axial direction by a support frame (not shown in the drawings). The arrangement and arrangement of the plurality of photosynthetic culture modules 11 and the illumination module 16 will be further described below in conjunction with FIG.

Each of the photosynthetic culture modules 11 is made of a light transmissive material, and each of the photosynthetic culture modules 11 has a top portion 11a, a bottom portion 11b, and a portion between the top portion and the bottom portion. A portion 11c has an inner diameter smaller than the top portion 11a and the bottom portion 11b. The top portion 11a and the bottom portion 11b of each of the photosynthetic culture modules 11 have a first opening 111 and a second opening 112, respectively.

The nutrient supply module 12 can include a plurality of first introduction tubes 121, and each of the first introduction tubes 121 can extend through the first opening 111 of each of the photosynthetic culture modules 11 respectively. Each of the photosynthetic culture modules 11 is incorporated. Therefore, the nutrient supply module 12 can be coupled to each of the photosynthetic culture modules 11 via each of the first introduction tubes 121.

The gas supply module 13 can include a plurality of second introduction tubes 131, and each of the second introduction tubes 131 can extend through the first opening 111 of each of the photosynthetic culture modules 11 respectively. Each of the photosynthetic culture modules 11 is incorporated. Therefore, the gas supply module 13 can be coupled to each of the photosynthetic culture modules 11 via each of the second introduction tubes 131.

The collection module 14 can include a plurality of collection tubes 141, and each of the collection tubes 141 can be coupled to the second opening 112 of each of the photosynthetic culture modules 11, respectively. Therefore, the collection module 14 can be coupled to each of the photosynthetic culture modules 11 via each of the collection tubes 141, respectively.

The gas detecting module 15 can include a first discharging module 151, and the first discharging module 151 can pass through a third opening 11g1 on the side of one of the 11g of the photosynthetic breeding module 11 The photosynthetic culture module 11g is extended. Therefore, the gas detecting module 15 can be coupled to the photosynthetic culture module 11g via the first exhaust module 151.

The light emitting module 16 can emit a light, and the light can illuminate and penetrate each of the photosynthetic culture modules 11. In an embodiment of the invention, the light emitting module 16 can be a spontaneous light source, such as a light source composed of a plurality of light emitting diodes. In another embodiment of the present invention, the light emitting module 16 can be a non-spontaneous light source, such as a module composed of a plurality of optical fibers, and the optical modules can introduce external light (such as sunlight). The light-emitting module 16 is steered to each of the photo-culture modules 11 . According to an embodiment of the present invention, each of the photosynthetic culture modules 11 has a nutrient solution 113 and a plurality of target cultures (not shown in the figure), and the nutrient solution 113 can supply the plurality of targets. The nutrients needed for the culture. Such target cultures may be animal cells, plant cells, and microorganisms such as algae, yeast, and the like. According to an embodiment of the invention, the illumination module 16 can adjust and set the wavelength of the emitted light to maintain the emitted light at a specific wavelength to facilitate the growth of the target culture. According to an embodiment of the invention, if the target cultures have a first type of photosynthesis system (PS-I, photosystem-I), such as Bule-green-algae (Spirulina platersis), blue-green (Cyanobacteria, Synechococcus sp.) and Photosynthetic bacteria (Rhodopesudomonas palustris), the light-emitting module 16 emits light having a red wavelength (about 660 nanometers (nm)) to facilitate the above-mentioned target cultures. Growth. Wherein the blue-green algae and the blue-green bacterium have a second type of photosynthesis system (PS-II, photosystem-II), so the light-emitting module 16 can also emit blue light wavelength (about 475 nm (nm)). ) the light.

In an embodiment in accordance with the invention, the nutrient supply module 12 has a nutrient solution 113 and a pump 122 that provide the target nutrient nutrient. The nutrient supply module 12 can supply the nutrient solution 113 to each of the first introduction tubes 121 via the pump 122, and the nutrient solution is passed through each of the first introduction tubes 121. The liquid 113 is introduced into each of the photosynthetic culture modules 11, respectively. The nutrient supply module 12 further includes a temperature control module 123 and an acid-base control module 124. The temperature control module 123 can control and maintain the temperature of the nutrient solution 113, and the acid-base control module 124 The pH of the nutrient solution 113 can be controlled and maintained. Through the temperature control module 123 and the acid-base control module 124, the nutrient solution 113 can be set to have conditions favorable for the growth of the target culture.

In an embodiment of the invention, the gas supply module 13 can provide the gas required for the growth of the target cultures cultivated in each of the photosynthetic culture modules 11. For example, when the target culture is algae, the gas can be the carbon dioxide required for photosynthesis of the algae. The gas can be introduced into each of the photosynthetic culture modules 11 via each of the second introduction tubes 131. The introduced gas can be agitated by the air-lift principle, and the nutrient solution 113 inside each of the photosynthetic culture modules 11 is stirred. The agitation effect formed by the gas lift principle will be further explained below in conjunction with FIG.

In an embodiment of the present invention, the target cultures within each of the photosynthetic culture modules 11 can be collected in the collection module 14 via each of the collection tubes 141. Since the nutrient solution 113 is attached to the target cultures collected, the target cultures must be separated from the nutrient solution 113. Therefore, the collection module 14 further includes a pump 143, and the pump 143 is further coupled to a centrifugal separator 142. The pump 143 continuously delivers the target cultures to which the nutrient solution 113 is attached to the centrifugal separator 142, and the centrifugal separator 142 separates the target cultures from the nutrient solution 113, and then Wait for the target culture to harvest. The centrifugal separator 142 can be coupled to a storage tank 144 that can store the harvested target species.

In an embodiment of the present invention, the gas detecting module 15 can detect the type and concentration of the gas discharged by the photosynthetic breeding module 11g via the first discharging module 151. For example, if the target culture cultured in the photosynthetic culture module 11g is algae, the gas discharged is the oxygen emitted by the algae for photosynthesis. Since the gas discharged from the photosynthetic culture module 11g is not connected to the gas discharged by each of the other photosynthetic culture modules 11, the gas detection module 15 is only for sampling the photosynthetic culture module 11g. The emitted gas is detected, and thus the type and concentration of the gas discharged by each of the other photosynthetic culture modules 11 are indirectly estimated, and the breeding conditions for each of the photosynthetic culture modules 11 are further Monitor

2 is a graphical representation of one of the photosynthetic culture modules 11 in accordance with an embodiment of the present invention. Referring to FIG. 2, each of the first introduction tubes 121 respectively extending into the first opening 111 of each of the photosynthetic culture modules 11 may further include a flow control valve 125. The flow throttle valve 125 can control the flow of the nutrient solution 113 from the nutrient supply module 12. In addition, each of the second introduction tubes 131 respectively extending into the first opening 111 of each of the photosynthetic culture modules 11 may further include a flow control valve 132, the flow rate The throttle valve 132 controls the flow of gas from the gas supply module 13 such that the gas is injected into each of the photosynthetic culture modules 11 at a rate.

Each of the second introduction tubes 131 can extend into the liquid surface 113a of the nutrient solution 113 in each of the photosynthetic culture modules 11, and each of the second introduction tubes 131 The tail end of the person may further include a diffusing element 133; by the diffusing element 133, the injected gas may cause agitation of the nutrient solution 113 by using an air-lift in the nutrient solution 113. Further, the injected gas can form a bubble 113b in the nutrient solution 113 by the diffusing element 133, and the bubble 113b moves upward and drives the nutrient solution 113 to circulate in the direction of 113c, thereby achieving the nutrition. The stirring action of the liquid 113. The agitation is such that the injected gas is sufficiently melted in the nutrient solution 113 to provide the target cultures cultured in each of the photosynthetic culture modules 11. Further, the stirring action allows the nutrient solution 113 to be uniformly dispersed. On the other hand, the agitation action further causes the nutrient solution 113 to generate a turbulent flow which inhibits the accumulation of organisms other than the target culture.

Since the inner diameter of the first portion 11c of each of the photosynthetic culture modules 11 is smaller than the top portion 11a and the bottom portion 11b, so that the photosynthetic culture modules 11 have a recess 11f, the recess 11f can be guided and The circulation of the nutrient solution 113 in the direction of 113c is enhanced, and the agitation is enhanced.

In addition, the gas discharged by each of the photosynthetic culture modules 11 can be connected to the outside gas through the first opening 111 of each of the photosynthetic culture modules 11; however, the photosynthesis The nutrient solution 113 in each of the culture modules 11 is incapable of communicating with the outside. Therefore, in the present invention, the photobiochemical reaction system 10 composed of the photosynthetic culture modules 11 belongs to a semi-open culture system. Its manufacturing cost is much lower than the fully enclosed farming system of the prior art. In contrast to the fully open culture system of the prior art, the culture conditions of each of the photosynthetic culture modules 11 of the photobiochemical reaction system 10 of the present invention (ie, the photosynthetic culture molds) The type and concentration of the gas in each of the groups 11 and the temperature and pH of the nutrient solution 113 are more easily controlled.

3 is a diagram of the photosynthetic culture module 11g in accordance with an embodiment of the present invention. Referring to FIG. 3, the first emission module 151 further includes a flow control valve 152 capable of controlling the flow of gas introduced into the first discharge module 151 by the photosynthetic culture module 11g. The gas emitted by the photosynthetic culture module 11g can be introduced into the gas detecting module 15 at a rate to facilitate the gas detecting module 15 to detect the emitted gas.

4 is a diagram showing the arrangement of a photobiochemical reaction system 10 in accordance with an embodiment of the present invention. Referring to FIG. 4, the photobiochemical reaction system 10 includes a plurality of layers of photosynthetic culture modules (first layer 11-1, second layer 11-2, and third layer 11-3), which can be stacked along the axial direction. In order to enable the photobiochemical reaction system 10 to set more of the photosynthetic modules 11 in a unit area on a radially defined plane, thereby increasing the yield of the target cultures in the unit area. In addition, the photobiochemical reaction system 10 of the present invention is disposed in a three-dimensional stacking manner as compared with the planar biochemical breeding system in the prior art, thereby saving the space required for setting the photobiochemical reaction system 10. In turn, the cost of land acquisition is reduced. However, those skilled in the art should be able to understand that the photo-culture module 11 included in the stereo-stack photobiochemical reaction system 10 of the present invention is not limited to three layers; In the case of a site height limitation of 10, more layers of the photosynthetic culture modules 11 can be arranged and stacked along the axial direction. The light-emitting module 16 can be formed into a rod-shaped light-emitting module or a long-length light-emitting module extending along the axial direction, in accordance with the plurality of layers of the photo-culture module 11 stacked along the axial direction. It is advantageous to provide a light source to each of the plurality of layers of photosynthetic culture modules 11. On the other hand, as shown in FIG. 4, more sets of stacked photobiochemical reaction systems can be disposed on a plane defined by the radial direction without exceeding the site area limit for setting the photobiochemical reaction system 10. For example, a second set of photobiochemical reaction systems 10-2 can be provided.

Figures 5A, 5C and 5D illustrate the arrangement and arrangement of photobiochemical reaction systems 10 in a plane defined by radial directions in accordance with an embodiment of the present invention. Referring to FIG. 5A, each of the photosynthetic culture modules 11 included in the photobiochemical reaction system 10 according to an embodiment of the present invention may be arranged in an annular manner on a plane defined by a radial direction to surround the luminescence. The module 16 and the annular shape may be circular; that is, each of the photosynthetic culture modules 11 has the same distance from the illumination module 16. Therefore, the light-emitting module 16 can provide an equal amount of light to each of the photo-culture modules 11 so that each of the photo-culture modules 11 has an equivalent culture environment to control and Maintaining the yield and quality of the target culture in each of the photosynthetic culture modules 11. Figure 5B shows the arrangement and arrangement of the culture module 20 and the illumination module 26 in a plane defined by the radial direction of the culture system 20 of the prior art. As shown in FIG. 5B, the light received by each of the photosynthetic culture modules 21 of the culture system 20 of the prior art is not equal, resulting in breeding in each of the photosynthetic culture modules 21. Environmental conditions are inconsistent and have a negative impact on the overall yield and overall quality of the target culture. For example, the light intensity of the photosynthetic culture module 21a is much lower than that of the other photosynthetic culture modules 21, and thus the photosynthetic culture module 21a has poor aquaculture environmental conditions, resulting in low yield and poor quality of the target culture. In addition, referring to FIG. 5A, in the photobiochemical reaction system 10 of the embodiment of the present invention, only a single illumination module 16 needs to be provided; in contrast, the culture system 20 in the prior art, as shown in FIG. 5B, needs to be provided with multiple The lighting module 26 provides light to each of the culture modules 21 and 21a. Therefore, the number of light-emitting modules required for the photobiochemical reaction system 10 of the embodiment of the present invention is low, and thus the facility cost can be reduced.

However, in the photobiochemical reaction system 10 of the present invention, the photosynthetic culture modules 11 are not limited to being arranged in a circular annular manner. Referring to FIG. 5C, in another embodiment of the present invention, the photosynthetic culture modules 11 can also be arranged in a square ring shape. Referring to FIG. 5D, in another embodiment of the present invention, the photosynthetic culture modules 11 are arranged in an annular manner of irregular polygons. However, the circular annular arrangement as shown in FIG. 5A enables each of the photosynthetic culture modules 11 to receive an equal amount of light; and it can be known from the basic geometrical principle that the circular ring shape The arrangement enables the maximum number of such photosynthetic culture modules 11 to be placed in a unit area on a plane defined by the radial direction, thereby saving land acquisition costs. Thus, as shown in Figure 5A, it is a preferred embodiment of the present invention.

Those skilled in the art should be aware that changes can be made to the above examples without departing from the broad inventive concepts. Therefore, it is understood that the invention is not limited to the specific examples of the invention, and is intended to cover the modifications of the invention and the scope of the invention as defined by the appended claims.

10. . . Photobiochemical reaction system

10-2. . . The second group of stacked photobiochemical reaction systems

11. . . Photosynthetic culture module

11-1. . . The first layer of photosynthetic culture module

11-2. . . Second layer photosynthetic culture module

11-3. . . The third layer of photosynthetic culture module

11a. . . Top of photosynthetic culture module

11b. . . Photosynthetic culture module bottom

11c. . . The first part of the photosynthetic culture module

11f. . . Sag of photosynthetic culture module

11g. . . Photosynthetic culture module

11g1. . . Third opening

111. . . First opening

112. . . Second opening

113. . . Nutrient solution

113a. . . Liquid level of nutrient solution

113b. . . bubble

113c. . . Circulating flow direction of nutrient solution

12. . . Nutrition supply module

121. . . First introduction tube

122. . . Pump

123. . . Temperature control module

124. . . Acid-base control module

125. . . Flow control valve

13. . . Gas supply module

131. . . Second introduction tube

132. . . Flow control valve

133. . . Diffusion element

14. . . Collection module

141. . . Collection tube

142. . . Centrifugal separator

143. . . Pump

144. . . Storage tank

15. . . Gas detection module

151. . . First emission module

152. . . Flow control valve

16. . . Light module

20. . . Conventional farming system

twenty one. . . Conventional technology breeding module

21a. . . Conventional technology breeding module

26. . . Light-emitting module of conventional technology

The foregoing summary of the invention, as well as the above detailed description For the purposes of illustrating the invention, various embodiments are shown in the drawings. However, it should be understood that the invention is not limited to the precise arrangements and devices disclosed.

In each figure:

1 is a diagram of a photobiochemical reaction system in accordance with an embodiment of the present invention;

2 is a pictorial representation of one of the photosynthetic culture modules in accordance with an embodiment of the present invention;

3 is a diagram of a photosynthetic culture module in accordance with an embodiment of the present invention;

FIG. 4 is a diagram showing the arrangement and arrangement of the photosynthetic culture modules and the illumination modules according to an embodiment of the invention;

5A, 5C, and 5D illustrate the arrangement and arrangement of each of the photosynthetic culture modules and the illumination module in a plane defined by the radial direction according to an embodiment of the present invention;

FIG. 5B shows the arrangement and arrangement of the culture module and the light-emitting module in the plane defined by the radial direction in the culture system of the prior art.

10. . . Photobiochemical reaction system

11. . . Photosynthetic culture module

11a. . . Top of photosynthetic culture module

11b. . . Photosynthetic culture module bottom

11c. . . The first part of the photosynthetic culture module

11g. . . Photosynthetic culture module

11g1. . . Third opening

111. . . First opening

112. . . Second opening

113. . . Nutrient solution

12. . . Nutrition supply module

121. . . First introduction tube

122. . . Pump

123. . . Temperature control module

124. . . Acid-base control module

13. . . Gas supply module

131. . . Second introduction tube

14. . . Collection module

141. . . Collection tube

142. . . Centrifugal separator

143. . . Pump

144. . . Storage tank

15. . . Gas detection module

151. . . First emission module

16. . . Light module

Claims (10)

A photobiochemical reaction system comprising: a plurality of photosynthetic culture modules, each of the photosynthetic culture modules having a top, a bottom, and a first portion between the top and the bottom, the first a portion of the inner diameter is smaller than the top portion and the bottom portion, and each of the photosynthetic culture modules is permeable; and a light emitting module that emits light of a specific wavelength, the light of the specific wavelength Irradiating and penetrating each of the light transmissive photosynthetic culture modules. The photobiochemical reaction system of claim 1, each of which has a nutrient solution inside, and the inner diameter of the first portion of each of the photosynthetic culture modules is smaller than the top portion and the bottom portion to form a Depression to enhance the circulation and agitation of the nutrient solution. The photobiochemical reaction system of claim 2, wherein each of the top and the bottom has a first opening and a second opening, and each of the photosynthetic breeding modules has a plurality of interiors. Target culture. The photobiochemical reaction system of claim 3, further comprising a nutrient supply module, wherein the nutrient supply module comprises a plurality of first introduction tubes, each of the first introduction tubes respectively passing the photosynthetic Each of the first openings of each of the culture modules extends into each of the photosynthetic culture modules, and the nutrient supply module is coupled to the photosynthesis via each of the first introduction tubes Each of the breeding modules. The photobiochemical reaction system of claim 4, further comprising a pump, wherein the nutrient supply module respectively delivers the nutrient solution to each of the first introduction tubes via the pump, and Each of the first introduction tubes introduces the nutrient solution into each of the photosynthetic culture modules. The photobiochemical reaction system of claim 3, further comprising a gas supply module, the gas supply module comprising a plurality of second introduction tubes, each of the second introduction tubes respectively passing the photosynthetic Each of the first openings of each of the culture modules extends into each of the photosynthetic culture modules, and the gas supply module is coupled to the photosynthesis via each of the second introduction tubes Each of the breeding modules. The photobiochemical reaction system of claim 6, wherein the gas is introduced into each of the photosynthetic culture modules via each of the second introduction tubes, and the introduced gas can be The nutrient solution inside each of the photosynthetic culture modules produces agitation. The photobiochemical reaction system of claim 3, further comprising a collection module, the collection module comprising a plurality of collection tubes, each of the collection tubes being coupled to each of the photosynthetic culture modules The second opening of the one, and the collection module can be coupled to each of the photosynthetic culture modules via each of the collection tubes. The photobiochemical reaction system of claim 8 which collects the target cultures in each of the photosynthetic culture modules in the collection module via each of the collection tubes . The photobiochemical reaction system of the first aspect of the invention includes a gas detection module, the gas detection module comprising a first emission module, and the first emission module via the photosynthetic breeding module One of the sides of the one of the sides extends into the third opening, and the gas detecting module is coupled to one of the photosynthetic breeding modules via the first exhaust module and discharged The type and concentration of the gas are detected.