KR101490325B1 - Photobioreactor of vinyl sheet type capable of interconnecting and method for installation the same - Google Patents

Abstract

The present invention can arrange a plurality of photosynthesis microorganisms in a vertical or horizontal form to enable the mass cultivation of photosynthetic microorganisms and to increase light transmittance by arranging a plurality of light transmitting portions formed on a reaction sheet in a window shape and connecting each reaction sheet through a connecting portion, The present invention relates to a vinyl-sheet type photo-bioreactor capable of wide-area connection and capable of harvesting the photosynthetic microorganisms at a time, and a culture space in which a photosynthetic microorganism and a culture medium are contained, A gas supply part for supplying carbon dioxide to the inside of the reaction sheet; and a gas supply part for introducing oxygen generated by the photosynthesis action of the photosynthetic microorganism in the carbon dioxide supplied into the inside of the reaction sheet, An inlet and an outlet for discharging the reaction product; And connection portions formed on both sides of the reaction sheet.

Description

TECHNICAL FIELD [0001] The present invention relates to a vinyl-type photobioreactor capable of wide-area connection, and a method of installing the same.

The present invention relates to a vinyl-sheet type photobioreactor capable of wide-area connection and a method of installing the same, and more particularly, to a method of installing a plurality of photosynthesis microorganisms in a vertical or horizontal configuration, The present invention relates to a vinyl-sheet-type photo-bioreactor and a method of installing the same. The vinyl-sheet-type photo-bioreactor has a light-transmittance and a light-transmitting property.

Global oil crises such as depletion of petroleum and natural gas and instability of supply and demand systems are being created. In addition, restrictions on the use of fossil fuels are becoming apparent in order to protect ecosystems such as climate change and environmental destruction.

As a result, countries around the world are striving not only to develop new and renewable energy, but also to increase the efficiency of existing thermal power generation and to raise the environment friendly. Biological energy production technology using photosynthetic microorganisms is also attracting attention.

In recent years, research on the use of photosynthetic microorganisms has focused on the production of biofuels for transportation, due to the increase in prices of cereal resources due to the production of biofuels and concerns about food resources. In addition to basic research on the genomes and genes of photosynthetic microorganisms, System research and other applied researches are on a large scale

Photosynthetic microorganisms can grow using water, carbon dioxide and sunlight, and they can be cultivated anywhere in wastelands, coasts, and oceans, and they do not compete with existing land crops in terms of land or space. Photosynthetic microorganisms accumulate large amounts of lipids (up to 70%) in vivo according to culture conditions, and oil (lipid) production per unit area is 50-100 times higher than conventional edible crops such as soybeans, Very high. Biodiesel, which is produced from photosynthetic microorganisms such as microalgae as a raw material, can reduce pollutants such as fine dust and sulfur compounds compared with conventional diesel fuel and is suitable as an environment friendly vehicle fuel.

Photosynthetic microorganisms can be cultivated in large quantities, and unlike edible crops, they can be harvested on a daily basis. In addition, photosynthetic microorganisms can directly absorb high-concentration carbon dioxide (15% level) in the by-product gas such as thermal power plants, so that the effect of reducing carbon dioxide is also great.

In addition, photosynthetic microorganisms (biomass) have received great interest as potential production materials for high value-added pharmaceuticals, pigments, cosmetics, nutrients for proteins and carbohydrates, and fine chemicals. Carotene, astaxanthin, whole-cell dietary supplements, whole-cell aquaculture feed, polyunsaturated fatty acids, heavy isotope labeled metabolites, phycoerythrin (fluorescent label), anticancer drugs, And pharmaceutical proteins, are being sold worldwide.

These technologies for producing high value-added products using photosynthetic microorganisms are largely composed of 1) cultivation of photosynthetic microorganisms, 2) harvesting, 3) extraction of useful substances, and 4) product conversion. The process of culturing double photosynthetic microorganisms is very important from the viewpoint of economical efficiency of the entire process. For example, microalgae cultivation, harvesting, oil extraction and biodiesel conversion processes cost 42%, 22%, 20% and 16%, respectively, for the microalgae biofuel production technology.

In order to efficiently produce photosynthetic microorganisms, development of high efficiency photobioreactors and high concentration culture techniques has been attempted, and methods of culturing photosynthetic microorganisms such as microalgae can be classified into two types, outdoor culture method and photo bioreactor method.

In the case of the outdoor culture method, for example, in the form of a pond or a water channel circulating the medium through the outer ring, it is difficult to cultivate a high concentration and to be contaminated by other microorganisms, thereby increasing the cost of recovering the photosynthetic product There are disadvantages.

Therefore, it is possible to produce high-value-added materials such as biofuels, pharmaceuticals, health foods and feeds using photosynthetic microorganisms, and in particular, a technology for mass-culture of photosynthetic microorganisms at a high concentration is required in the biological CO ₂ immobilization process, Demand for photobioreactors is increasing.

As a background art of the present invention, there is known a "bubble top photobioreactor" of Korean Patent No. 0439971 (filed on September 18, 2002), which forms a chamber A transparent jacket provided on the outer surface of the transparent jacket for separating the light emitting body from the culture liquid and allowing heat exchange; A baffle plate that forms a circulation path by dividing a rising portion and a descending portion of the baffle plate and an aeration device that feeds the gas upward at a lower end portion of one portion divided by the baffle plate to cause upward flow of the culture liquid.

Therefore, it is possible to circulate the culture liquid without any stirring, minimize the light transmission distance to the circulating culture medium, and transmit the light energy to the maximum surface area of the culture liquid, which is suitable for high concentration cultivation of photosynthetic microorganisms.

On the other hand, in countries where installation sites such as Korea are lacking, there is a high possibility of practical application of a photobioreactor in which cylindrical columns are arranged vertically in order to increase light utilization efficiency.

However, the conventional photobioreactor using a cylindrical column is difficult to mass-scale due to high installation cost and management manpower due to the complexity of the reactor structure, and it is difficult to mass-culture the photosynthetic microorganisms according to the cultivation of individual columns.

Prior art related to this is disclosed in Korean Patent Laid-Open Nos. 10-2011-0085428, 10-2011-0137314, 10-2011-0062623, 10-0622992 There is a call.

The present invention can arrange a plurality of photosynthesis microorganisms in a vertical or horizontal form to enable the mass cultivation of photosynthetic microorganisms and to increase light transmittance by arranging a plurality of light transmitting portions formed on a reaction sheet in a window shape and connecting each reaction sheet through a connecting portion, The present invention is to provide a vinyl-sheet type photo-bioreactor capable of wide-area connection to be able to harvest infusion and photosynthetic microorganisms at a time, and a method for installing the same.

The technical objects to be achieved by the present invention are not limited to the above-mentioned technical problems.

In order to achieve the above object, there is provided a vinyl-sheet type photobioreactor capable of wide-area connection, comprising a culture space in which a photosynthetic microorganism and a culture medium are contained, and the front and rear surfaces are bonded by thermal bonding to form a plurality of transmission portions An inlet and an outlet for discharging the oxygen generated by the photosynthesis action of the photosynthetic microorganism in the carbon dioxide supplied to the inside of the reaction sheet; And connecting portions formed at both sides of the reaction sheet so that the reaction sheets are connected in series.

Specifically, the reaction sheet is made of polyethylene (PE), low density polyethylene (LDPE), linear low density polyethylene (LLDPE), high density polyethylene (HDPE), oriented polypropylene (OPP), polyethylene terephthalate, CPP), unleaded polypropylene (CPP), nylon, biaxially oriented nylon (ON), unstretched nylon (CN), polyacetal (POM), polycarbonate (PC), polyester, polystyrene (PVP), Ethylene vinyl acetate copolymer (EVA), Polyethylene terephthalate (PET), Polypropylene (PP), Inflated Polypropylene (IPP) ) And polyphenylene oxide (PPO = PPE) or a material in which the above materials are combined.

The transmissive portion may be formed in a circular, elliptical, or polygonal shape, and may be formed in a continuous or zigzag arrangement in upper, lower, left, and right sides.

The transmitting portion may be formed in a polygonal shape that does not have a droplet portion on the top so that the photosynthetic microorganism does not become a droplet, and may be formed in a continuous or zigzag arrangement.

A through hole may be formed at the center of the transmissive portion.

Wherein the gas supply unit includes a supply pipe connected to the reaction sheet, a bubble tube connected to the supply pipe and having a plurality of pores inserted into a lower side of the reaction sheet to supply gas supplied from the supply pipe in the form of bubbles, And a supply pump installed at one side of the supply pipe to pump carbon dioxide into the reaction sheet.

Wherein the inlet and outlet are formed on one side of the reaction sheet and include an inlet and an outlet for injecting the culture liquid and a photosynthetic microorganism and discharging oxygen generated during the growth of the photosynthetic microorganism and a discharge pipe connected to one side of the inlet and outlet, And a discharge pump installed at one side of the discharge pipe to pump oxygen to the outside.

At the lower end of the reaction sheet, a discharge port for discharging photosynthetic microorganisms inside the reaction sheet may be formed.

A reflective plate may be provided at a position spaced apart from the lower side of the reaction sheet to reflect light and reflect the light onto the reaction sheet.

A thermal camera capable of photographing a temperature change of the reaction sheets is installed at a predetermined height of a predetermined distance from the reaction sheet, and the thermal camera is connected to a monitoring device to detect a temperature change of the reaction sheet have.

In order to accomplish the above object, the present invention provides a method of installing a vinyl sheet type photo bioreactor capable of wide-area connection, comprising the steps of: forming a culture space in which a photosynthetic microorganism and a culture medium are contained, Preparing a reaction sheet made of a vinyl material having a permeable portion formed thereon, arranging the upper and lower portions of the reaction sheet in a vertical or horizontal shape by supporting the upper and lower portions of the reaction sheet by a support, and connecting the reaction sheets through a connection portion Providing a gas supply part for supplying carbon dioxide to a culture space of the reaction sheet, and installing an inlet and an outlet for discharging oxygen generated in the culture space of the reaction sheet.

As described above, the present invention can mass-culture the photosynthetic microorganisms and reduce the installation and management costs by arranging a plurality of the vinyl reaction sheets in a vertical or horizontal form, Are arranged in a window shape to increase the light transmittance and increase the productivity.

Since a plurality of reaction sheets are arranged in series and connected through a connection portion, the culture liquid and the microorganism can be injected or discharged at once, and the photosynthetic microorganism can be discharged at a time.

1 is a perspective view showing the overall configuration of a vinyl sheet type photobioreactor capable of wide-area connection according to an embodiment of the present invention.
2 is an enlarged view of a portion 'A' in FIG.
3 is a front view showing that a vinyl sheet type photobioreactor according to the present invention is installed in parallel.
FIG. 4 is an enlarged cross-sectional view of the connecting portion 'B' of FIG. 3 in the reaction sheet according to the present invention.
FIG. 5 is a view showing that the transmission portion of the reaction sheet according to the present invention is formed into a polygonal shape and a semicircular shape.
6 is a view showing a plurality of reaction sheets according to the present invention arranged in parallel in a fixing device.
7 is an enlarged view of a portion 'C' of FIG.
Fig. 8 is a view showing that a plurality of reaction sheets according to the present invention are arranged in parallel in a fixing device, and a transmission hole is formed in the transmission portion of the reaction sheet.
9 is a view showing that the transmissive portion of the reaction sheet according to the present invention is formed in a circular shape and is formed to be shifted from the transmissive portion of the reaction sheet arranged in parallel.
10 is a view showing that the transmissive portion of the reaction sheet according to the present invention is formed in a triangular shape and is formed to be shifted from the transmissive portion of the reaction sheet arranged in parallel.
11 is a view showing that the transmission portion of the reaction sheet according to the present invention is formed in a polygonal shape and is formed to be shifted from the transmission portion of the reaction sheet arranged in parallel.
12 is a view showing that the transmission portion of the reaction sheet according to the present invention is formed long in a polygonal shape.
13 is a flowchart showing a method of installing a vinyl sheet type photobioreactor according to the present invention.
14 shows photographs of a vinyl sheet type photobioreactor capable of wide-area connection of the present invention.
15 shows a photograph and a schematic diagram of a multi-cylinder structured plate-type transparent film photobioreactor.
Figure 16 shows the change in the cell concentration of KR-1 according to the plate-type photobioreactor structure (vinyl sheet type vs. multi-cylinder structure).
17 shows the change in the amount of KR-1 cells per reactor according to the plate-type photobioreactor structure (vinyl sheet type vs. multi-cylinder structure).
Figure 18 shows photographs of vinyl sheet type photobioreactors of various thermally bonded surfaces. (A) When the heat bonding surface is translucent, (B) When the aluminum foil is attached, and (C) When the heat bonding portion is cut out to form a transparent structure. The reactor order is 1, 2, 3, 4 from the front.
Fig. 19 shows the difference in light irradiation amount depending on the type of thermally bonded surface and the incubation time. The light dose was measured at the midpoint between the first reactor 1 and the second reactor (see FIG. 18).
Fig. 20 shows the growth of KR-1 according to the thermocomposite surface morphology.
21 shows the volume of the culture liquid and the reactor thickness according to the ratio of the thermal interface area.
22 shows the change in the cell concentration of KR-1 according to the ratio of the heat-bonded area.
FIG. 23 shows the productivity and productivity of KR-1 according to the ratio of thermocomposite surface.
24 shows a photograph of the dead zone observed at the top of the elliptical thermal interface.
25 shows the change of the cell concentration of KR-1 according to the gas supply rate in the vinyl sheet type photobioreactor.
FIG. 26 shows photographs of a vinyl sheet type photobioreactor having thermoelectric junctions of type (A) and type (B).
FIG. 27 shows the relative productivity and yield of KR-1 in a vinyl sheet type photobioreactor having thermal conductivity type (A) and type (B).
FIG. 28 shows photographs of reactors having lengths of 1.5 m, 2.0 m and 2.5 m.
Figure 29 shows the culture volume of the reactor of each length.
30 shows the relative unit volume and the cell productivity per unit area in the reactor of each length.
FIG. 31 shows changes in the cell concentration of KR-1 with time.
Figure 32 shows changes in lipid content of KR-1 over time.
Figure 33 shows changes in lipid productivity of KR-1 over time.
34 shows intensity and temperature change of natural light irradiated to the greenhouse during semi-continuous cultivation.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. In the drawings, the same components are denoted by the same reference symbols whenever possible. In the following description, well-known functions or constructions are not described in detail since they would obscure the invention in unnecessary detail.

1 to 3 are views showing a vinyl sheet type photo bioreactor according to an embodiment of the present invention. The reaction sheet 10, the gas supply unit 20, the inlet / outlet unit 30, the transmission unit 12, .

The reaction sheet 10 is made of a vinyl material and has a culture space 11 in which a photosynthetic microorganism and a culture medium are contained, and a plurality of transparent portions 12 are formed on the front and back surfaces by thermal adhesion.

The transmitting portion 12 may be formed in a circular, semicircular, elliptic or polygonal shape so that the photosynthetic microorganisms are not stained and contaminated.

(A) polyethylene (PE), (b) low density polyethylene (LDPE), (c) polyethylene terephthalate, and the like. (D) High density polyethylene (HDPE), (e) Oriented polypropylene (OPP), (f) Polyethylene terephthalate and unleaded polypropylene mixed film (PET + CPP) Polypropylene (CPP), (h) nylon, (i) biaxially oriented nylon (ON), (j) unstretched nylon (CN), (k) polyacetal (POM) ), (m) polyester, (n) polystyrene (PS), (o) polyester sulfone (PES), (p) polyvinyl chloride (PVC), (q) vinylidene chloride r) Ethylene vinyl acetate copolymer (EVA), (s) Polyethylene terephthalate (PET), (t) Polypropylene, (u) Inflated Polypropylene (IPP), and (v) Polyphenylene oxide (PPO = PPE) Or or of the material that may be formed of a composite material.

The reaction sheet 10 formed in this manner is advantageous in that it has light transmittance, lightness and transparency, and excellent economy, compared to glass, acrylic, etc., which are widely used as a reaction vessel material of a photosynthetic bioreactor.

The photosynthetic microorganisms injected into the culture space 11 of the reaction sheet 10 are classified into microalgae, cyanobacteria and photosynthetic bacteria.

Here, the microalgae are collectively referred to as single-cell eukaryotic microorganisms that photosynthate with photosynthetic pigments.

Examples of the microalgae include Anacystis nidulans , Ankistrodesmus sp. , Biddulpha aurita , Botryococcus braunii , Cathothorous sp. Chaetoceros sp.), Chlamydomonas Oh Plata appear (Chlamydomonas applanata), Chlamydomonas lane Hearty (Chlamydomonas reinhardtii), chlorella (chlorella sp.), chlorella ellipsis Soy Boutique (chlorella ellipsoidea), chlorella Emma Sony (chlorella emersonii), chlorella prototype Te koi death (chlorella protothecoides), chlorella Pierre Noi guru (chlorella pyrenoidosa), chlorella Thoreau Kearney Ana (chlorella sorokiniana), chlorella vulgaris (chlorella vulgaris), chlorella minu Tea Island (chlorella minutissima), chloro Caucus Margarito ralre (Chlorococcus littorale , Cyclotella cryptica , Dunaliell < RTI ID = 0.0 > a bardawil), two Nari Ella Salina (Dunaliella salina), two Nari Ella Hotel Tio rekta (Dunaliella tertiolecta), two Nari Ella Primo rekta (Dunaliella primolecta), Jim nodi num (Gymnodinum sp.), Himeji Nomo eggplant carbonara Terra (Hymenomonas Carterae , Isochrysis galbana , Isochrysis sp. , Microcystis aeruginosa , Micromonas pusilla , Monodus suberanthus, subterraneous , Nannochloris sp. ), Nannochloropsis sp. , Nannochloropsis atomus , Nannochloropsis salina , Navicula pelliculosa , Nitzschia sp. , Nitzscia closterium , Nitzscia palea , Oocystis polymorpha , Ourococcus sp. , Oscillatoria rubescens , ruteri (Pavlova lutheri), L ohdak tilrum tree cor nutum (Phaeodactylum tricornutum), peak no Lactococcus Pro bar Solid (Pycnococcus provasolii), minnow Pseudomonas koreuda other (Pyramimonas cordata), Spirulina platen sheath (Spirulina platensis), Stefano discharge kusu minu Toulouse (Stephanodiscus minutulus), styryl Coco carcass (Stichococcus sp.), dryer cine ulna (Synedra ulna), three or four O Bulletin des mousse kusu (Scenedesmus obliquus), Selena Teurum Gras silane (Selenastrum gracile), skeletal retrograde Norma course talrum (Skeletonoma costalum), tetra-cell Miss Tsui (Tetraselmis chui), tetra-cell misses Marcoola other (Tetraselmis maculata), tetra-cell Miss (Tetraselmis sp.), Tetra-cell Miss Tetraselmis suecica , Thalassiosira pseudomona , and the like. In the present invention, one or more of the microalgae may be used.

The cyanobacteria are collectively referred to as photosynthetic bacteria using chlorophyll in prokaryotes.

Such the cyanobacteria will know vena (Anabaena sp.), With a knife matrix (Calothrix sp.), Cami chiffon (Chaemisiphon sp.), Chroma Nose CD option system (Chroococcidiopsis sp.), Cyano tese (Cyanothece sp.), Published DE Such as Cylindrospermum sp. , Dermocarpella sp. , Fischerella sp. , Gloeocapsa sp. , Myxosarcina sp. , Nostoc sp. ), Oscillatoria sp. , Phormidium corium, Pleurocapsa sp. , Prochlorococcus sp. , Pseudanabaena sp. , Cinecococcus sp. Synechococcus , Synechocystis sp. , Tolypothrix sp. , Xenococcus sp. And the like. In the present invention, one or more of the above-mentioned species can be used.

The photosynthetic bacteria refer to bacteria that utilize light energy to assimilate carbon.

Such photosynthetic bacteria include, but are not limited to, Rhodocista centenaria , Rhodospira trueperi , Rhodospirillum fulvum , Rhodospirillum molischianum , Rhodospirillum photomultikum , Such as Rhodospirillum photometricum , Rhodospirillum rubrum , Rhodospirillum salexigens , Rhodospirillum salinarutn , Rhodospirillum sodomense , Rhodospirillum sodomense , Medio raised num (Rhodospirillum mediosalinum), also Pseudomonas (Rhodopseudomonas sp.), also Pseudomonas ash FIG pillar (Rhodopseudomonas acidophila), also Pseudomonas capsule la tooth (Rhodopseudomonas capsulatus), also Pseudomonas Palouse tris (Rhodopseudomonas palustris), also Pseudomonas seupaeroyi death (Rhodopseudomonas sphaeroides), Rhodobacter capsule Tooth and the like (Rhodobacter capsulatus), Rhodobacter seupaeroyi des (Rhodobacter sphaeroides), can be used one or more of the photosynthetic bacterium in the present invention.

Because they have bacteriochlorophyll instead of chlorophyll, photosynthetic growth of carbon dioxide is possible.

The reaction sheet 10 can partially immerse the photosynthetic microorganism in the culture space 11 and water having a constant temperature so as to keep the temperature of the culture liquid constant. At this time, it is possible to immerse a part of the reaction sheet 10 by storing water in a separate container for immersing the reaction sheet 10 in water. In addition, the reaction sheet 10 may be partially immersed in seawater, which is a natural environment. When the natural environment such as seawater is used, it is a method that can be used when the reaction sheet 10 is installed on a large scale.

In order to raise the temperature of the reaction sheet 10, a reflector 90 for reflecting sunlight may be provided under the reaction sheet 10 to reflect light to the reaction sheet 10.

The reflection plate 90 is installed with an inclination angle so that light can be reflected and reflected.

At the upper end of the reaction sheet 10, an insertion hole 14 is formed in the horizontal length direction, and the support table 40 is inserted. The support 40 fixes the reaction sheet 10 to the fixing device 70 so that the reaction sheet 10 is spaced apart from the bottom.

At this time, the fixing device 70 is fixed to the floor.

The reaction sheet 10 is deformed when the culture liquid and the photosynthetic microorganism are injected into the culture space 11 of the reaction sheet 10 by forming the fixing holes 15 in the horizontal length direction on the lower side of the reaction sheet 10 The fixing table 50 is inserted.

A discharge port 60 is formed between the fixing hole 15 and the culture space 11 of the reaction sheet 10 to discharge the culture medium of the photosynthetic microorganism that has been grown in the culture space 11 of the reaction sheet 10 to the outside have.

The discharge port 60 is provided with a valve 61 to open and close the discharge port 60 through the valve 61. When the culture liquid is injected into the reaction sheet 10, the valve 61 is closed to close the discharge port 60. When discharging the cultured photosynthetic microorganism liquid, the valve 61 is opened to discharge the discharge port 60 Open.

As shown in FIGS. 1 to 5, the transparent portion 12 is circular, elliptical, triangular, or polygonal, and is continuously arranged vertically and horizontally without a droplet (not shown) in which circular or photosynthetic microorganisms are embedded, The production efficiency of the photosynthetic microorganism can be improved by supplying light also to the reaction sheet 10 provided on the rear side of the plurality of reaction sheets 10 when the light is transmitted through the transmission portion 12.

The arrangement of the transmitting portions 12 can be modified as much as possible so that each of the plurality of reaction sheets 10 vertically arranged can also be formed of different arrangements of the transmitting portions 12, ) Can also supply light.

The most preferred arrangement of the transmissive portions 12 is a zigzag arrangement.

The transmissive portion 12 may be formed by removing a predetermined portion of the transmissive portion 13 on the inner side. So that light is transmitted through the through hole 13 and irradiated to another reaction sheet 10 spaced apart from the reaction sheet 10 by a predetermined distance.

The through hole 13 may be formed in an area of 50 to 90% of the total area of the transmissive portion 12. This is because when the area of the through hole 13 is less than 50%, the through hole is small and the light transmittance and ventilation functions are poor. When the area of the through hole 13 exceeds 90% It can be damaged.

By using the transparent vinyl reaction sheet 10, it is easy to install in the field, and the cost for installation and management can be reduced.

The ratio of the pressing surface of the transmitting portion 12 to the total area of the reactive sheet is in the range of 5 to 35%. This is because if the ratio of the pressing face is less than 5%, the reaction sheet 10 may be damaged due to its weight over time as the photosynthetic microorganism grows in the reaction sheet 10, and the thickness of the reaction sheet becomes thick This is because the growth of photosynthetic microorganisms may be deteriorated.

If the ratio of the squeezed surface of the transparent portion 12 is more than 35%, the ratio of the pressed portion of the reactive sheet 10 is increased to reduce the space in which the photosynthetic microorganism can grow, thereby reducing the culture space .

These transmissive portions 12 are formed as small as possible so that the culture space 11 is increased to increase the number of the transmissive portions 12 so as to prevent the breakage of the reaction sheet 10 due to the weight .

As shown in FIG. 4, a plurality of reaction sheets may be connected in series, and connection portions 80 are formed on both sides of the reaction sheet 10 to connect the reaction sheets.

The connection part 80 formed on one side of the reaction sheet 10 is formed of a protrusion type connection part 81 so as to be connected to the other reaction sheet 10 and the other side is formed on one side of the other reaction sheet 10 Like connecting groove 82 into which the connecting body 81 is inserted and connected.

Since the plurality of reaction sheets 10 can be connected in series by the connection unit 80, a plurality of reaction sheets 10 are connected at a time, and the culture solution and the microorganisms are injected into the entire reaction sheet 10 To simplify the input process.

In addition, since the microorganism can be connected to the connection unit 80 through the connection unit 80, the microorganism can be transferred to the culture solution, and thus the photosynthetic microorganism can be harvested at a time.

A valve 83 is installed in the connection part 80 to prevent the culture solution or oxygen from being discharged to the outside through the connection part 80 when the reaction sheets 10 are connected or disconnected.

The valve 83 may be formed in the form of a clamp to be coupled to the outside of the connection portion 80 to press the pipe having the connection portion 81 and the connection groove 82 of the connection portion 80, And the connection groove 82 are formed, and can be formed so as to open and close the pipe through rotation.

When the valve 83 is formed through the pipe of the connection part 80, a through hole is formed in the horizontal direction so that the valve 83 can be opened and closed through rotation.

The valve 83 is formed only in the pipe on the side of the connecting body 81 of the connecting portion 80 and formed on the side of the connecting groove 82 in the same manner as the air inlet of the tube, Or oxygen, so as not to flow back through the connecting groove 82.

The gas supply unit 20 supplies carbon dioxide to the inside of the reaction sheet 10 and includes a supply pipe 21 connected to the reaction sheet 10 and a supply pipe 21 connected to one side of the supply pipe 21, And a bubble tube 22 connected to the feed pipe 21 and inserted into the reaction sheet 10. The feed pipe 24 feeds the feed gas to feed the reaction space 10 to the culture space 11 of the reaction sheet 10,

The gas supplied through the gas supply unit 20 is supplied into the reaction sheet 10 in an amount of 0.01 to 0.3 VVM.

If the supply amount of the gas is less than 0.01 VVM, the growth of the photosynthetic microorganism is slowed because the photosynthetic microorganism inside the reaction sheet 10 does not reach the gas concentration for growth, , The photosynthetic microorganism inside the reaction sheet 10 exceeds the supply amount of the required gas, so that the growth of the photosynthetic microorganism can be slowed due to the excessively injected gas, and the photosynthetic microorganism can be inhibited by the shear stress The growth of microorganisms may be inhibited.

The bubble tube 22 of the gas supply unit 20 may be made of any one of stainless steel, polysulfone, polyethersulfone, polyvinylidene fluoride, Teflon, polyethylene, polypropylene, have.

The bubble tube 22 has a pore size of 0.01 to 300 μm. When the pores 23 of the bubble tube 22 are formed to be less than 0.01 μm, the gas supplied into the reaction sheet 10 is not properly supplied, so that the photosynthetic microorganisms in the reaction sheet 10 can not grow, It induces shear stress in photosynthetic microorganisms and inhibits growth. On the other hand, when the pores 23 of the bubble tube 22 are formed to have a pore size of more than 300 袖 m, a too large bubble size is formed and the gas is not smoothly supplied into the reaction sheet 10 due to the limitation of the gas-liquid reaction, The growth of photosynthetic microorganisms is inhibited by the limitation of nutrients such as nitrogen source.

The bubble tube 22 may be inserted into the reaction sheet 10 in plural to maintain the amount of gas supplied depending on the size of the reaction sheet 10.

The bubble tube 22 can be manufactured by adjusting the thickness of the bubble tube 22 by distance in order to maintain the amount of supplied gas according to the size of the reaction sheet 10 and to prevent pressure loss.

That is, the bubble tube 22 is formed such that the diameter of the bubble tube 22 gradually decreases in the longitudinal direction in the direction opposite to the portion connected to the supply pipe 21.

This is because bubbles are generated in a portion of the bubble tube 22 connected to the supply pipe 21 because the pressure is strong. However, since the gas supply pressure decreases toward the opposite side, the diameter is gradually decreased .

In addition, the pores formed in the bubble tube 22 may be formed to be narrower in the interval from the portion where the supply pipe 21 is connected to the opposite side.

The cross section of the bubble tube 22 is not limited to a circular shape but may be formed in various shapes such as an ellipse, a quadrangle, a triangle, and a polygon, and may be formed by adjusting the interval of pores formed in the bubble tube 22.

An additional supply pipe 21 and a supply pump 24 may be provided on both sides of the bubble tube 22 in order to maintain the amount of gas supplied according to the size of the reaction sheet 10 in the bubble tube 22.

The input / output unit 30 discharges oxygen generated by the photosynthesis action of photosynthetic microorganisms such as microalgae from the carbon dioxide supplied to the inside of the reaction sheet 10, and injects the culture liquid and the photosynthetic microorganisms into the reaction sheet 10 And is connected to an inlet 31 formed on one surface of the reaction sheet 10 and a discharge pipe 32 connected to one side of the inlet 31 and the other side extending to the outside. A discharge pump 33 may be installed in the discharge pipe 32 for pumping the oxygen generated by the photosynthesis of the microorganism to the outside smoothly.

The culture solution and the photosynthetic microorganism can be injected into the reaction sheet 10 through the inlet 31 of the inlet 30. The exhaust pipe 32 may be connected to the inlet / outlet 31 to exhaust the oxygen generated when the photosynthetic microorganism injected into the reaction sheet 10 grows.

As shown in FIGS. 6 to 11, a plurality of the reaction sheets 10 may be installed in parallel to the fixing device 70 fixed on the floor.

The permeable portions 12 of each of the reaction sheets 10 arranged in parallel are formed in a side-by-side staggered arrangement. The reason is that light is transmitted through the transmission portion 12 of the reaction sheet 10 to illuminate the culture space 11 of another reaction sheet 10 arranged in parallel.

As shown in FIG. 12, the transmitting portion 12 may be formed long in the vertical direction of the reaction sheet 10. When the transparent portion 12 is formed in such a long form, the amount of light transmitted increases, and the growth of photosynthetic microorganisms in the other reaction sheets 10 arranged in parallel can be increased.

A thermal image of the entire area of the wide area of the reaction sheet 10 installed by installing the thermal imaging camera 100 at a predetermined height at a position spaced apart by a predetermined distance after the reaction sheets 10 are widely installed in series and in parallel The sensor 10 may be connected to the monitoring device 101 to detect a breakage or an abnormality of the reaction sheet 10 by detecting a change in the temperature of the reaction sheet 10 and the reaction sheet 10.

Hereinafter, a method of installing the vinyl sheet type photobioreactor according to the present invention will be described.

13, a method of installing a vinyl sheet type photobioreactor according to the present invention includes a step S100 of preparing a reaction sheet 10 having a transmission portion 12, a step S200 of setting up a reaction sheet 10 A step S300 of connecting the reaction sheets, a step S400 of installing the gas supply unit 20, and a step S500 of installing the input / output unit 30.

As shown in FIGS. 1 to 3 and 13, in step S100 of preparing the reaction sheet 10, a reaction sheet 10 made of a vinyl material, in which a culture space 11 in which a photosynthetic microorganism and a culture liquid are contained is formed, ).

In step S100 of preparing the reaction sheet 10, a reaction sheet having a plurality of transparent portions 12 formed by thermal bonding is prepared on the front and rear surfaces of the reaction sheet 10, The transmissive portion may be formed in a polygonal shape having no circular or elliptical shape or a triangular, pentagonal or ridge shape (not shown), and may be arranged continuously or zigzag in the upper, lower, left, and right directions, or in the form of a long polygon They may be arranged continuously or in a zigzag manner.

In the step of installing the reaction sheet 10 (S200), a plurality of the reaction sheets 10 are vertically or horizontally arranged in parallel by supporting the upper part of the reaction sheet 10 on the fixing device 70 via the support table 40, Compared with columnar photobioreactors, photosynthetic microorganisms can be cultured and the photosynthetic microorganisms can be rapidly recovered, thereby enabling large-scale cultivation of photosynthetic microorganisms.

The step of connecting the reaction sheets 10 (S300) is a step of connecting the reaction sheets 10 installed in series through the connection part 80.

The reaction sheet 10 is connected to the connection member 81 of the connection unit 80 and the connection groove 82 of the other reaction sheet 10 and is connected when the reaction sheet 10 is not connected, So that the reaction sheet 10 is sealed.

In step S400 of installing the gas supply unit 20, carbon dioxide is supplied to the culture space 11 of the reaction sheet 10, and the gas supply unit 20 is connected to the reaction sheet 10 A bubble tube 22 connected to the supply pipe 21 to supply gas into the reaction sheet 10 and a gas supply pipe 21 provided on one side of the supply pipe 21 for supplying gas to the reaction sheet 10 And a feed pump 24 for feeding the cultivation space 11 into the culture space 11.

At this time, a plurality of the bubble tubes 22 may be provided depending on the size of the reaction sheet 10.

In the step of installing the gas supply unit 20, gas is supplied to the bubble tube 22 through the supply pipe 21 and gas is bubbled through the bores 23 of the bubble tube 22, .

In step S500 of installing the inlet 30, the culture liquid and the photosynthetic microorganism are injected into the reaction space 10 of the reaction sheet 10 into the reaction space 10, and the oxygen generated during the growth of the photosynthetic microorganism The inlet 30 includes an inlet 31 formed in one surface of the reaction sheet 10 and a discharge pipe 32 connected at one end to the inlet 31 and the other end extending to the outside, . A discharge pump 33 for pumping the oxygen generated by the photosynthesis in the carbon dioxide to the outside can be installed on one side of the discharge pipe 32.

In the step S500 of installing the inlet 30, the culture liquid and the photosynthetic microorganism are injected into the reaction sheet 10 through the inlet 31 formed in the reaction sheet 10, The oxygen generated during the growth of the photosynthetic microorganism is discharged to the outside through the gas diffusion layer 32. The discharge pipe (33) may be connected to the discharge pipe (32) to discharge the oxygen to the discharge pipe (32) connected to the inlet (31).

After the reaction sheet 10 is installed, a reflective plate 90 for reflecting light is provided on the lower portion of the reaction sheet 10 so that external light is reflected to the reaction sheet 10, It can supply a lot of light.

The reaction sheet 10 and the reaction sheet 10, which are installed in a wide range in series and in parallel and are installed at a predetermined distance from the thermal imaging camera 100 at a predetermined height, And the temperature of the reaction sheet 10 and the reaction sheet 10 are detected by detecting the temperature change of the reaction sheet 10 and the reaction sheet 10 10) in the case of a failure.

Example 1: Experimental Materials and Methods

1) photosynthetic microorganisms and culture conditions

For photosynthetic microorganism cultivation using a vinyl sheet type photobioreactor, Chlorella sp. KR-1 (KCTC0426BP) strain was used, and N8 medium with nitrate concentration of 1 mM was used. The composition of the N8 medium was KNO ₃ (0.1011 g / L), KH ₂ PO ₄ (0.7400 g / L), Na ₂ HPO ₄ (0.2598 g / L), MgSO ₄ .7H ₂ O _{2 (0.0132g / L), FeNaEDTA} (0.0100g / L), ZnSO 4 · 7H 2 O (0.0032g / L), MnCl 2 · 4H 2 O (0.0130g / L), CuSO 4 (0.0117g / L) , And Al ₂ (SO ₄ ) ₃揃 18H ₂ O (0.0070 g / L).

KR-1 strain was preliminarily cultured in a constant temperature chamber equipped with a fluorescent lamp using a solid medium, a 250 mL flask, a 1L cylindrical glass photobioreactor, a 7L cylindrical glass photobioreactor, and then inoculated at 10% level of the present culture medium . In the 1L and 7L cylindrical glass photobioreactors, the CO ₂ concentration of the feed gas was 10% (v / v) and the gas feed rates were 0.3L / min and 0.75L / min, respectively. Temperature of the growth chamber interior constant temperature is 27 ~ 32 ℃, was the intensity of the light is ^{135 ~ 197 μmol / m 2 /} s.

Vinyl sheet type photobioreactor experiment was carried out under natural conditions in an outdoor glasshouse built on the roof of the flue gas R & D demonstration facility of Korea Institute of Energy Research or in a room temperature incubator. The CO ₂ concentration of the supplied gas was 10% (v / v), and the feed rate and VVM (volume of air added to the liquid volume per minute) were differently applied depending on the culture volume and reactor structure.

2) Production method of vinyl sheet type photobioreactor

Window-structure transparent film sheet photobioreactor is made by thermally bonding a polyethylene film (width 80 cm, length 150 cm, thickness 0.08 mm) with semi-elliptical heat plate (area 72 cm 2) (Fig. 14).

The temperature of the hot plate was kept at 160 ~ 170 ℃ during thermal bonding. Semi - elliptical thermal junctions were arranged in zigzag to facilitate the distribution of the supplied gases in the photobioreactor and the light transmittance.

The thermal bonding area was adjusted to 24 to 36% of the total area of the polyethylene film by changing the interval and the number of semi-elliptical thermal bonding surfaces. The interval between semi - elliptical thermal interface was 5 ~ 7cm in left and right and 3 ~ 9cm in upper and lower. The gas was discharged through the two openings located in the upper layer of the vinyl sheet type photobioreactor and the culture solution was supplied. The gas supply was provided through the entrance at the bottom left of the reactor and through a metal membrane (metal membrane, 68 cm long, 0.1 μm pore) inside the reactor, and a photosynthetic microorganism sample was collected through the entrance at the lower right. Unless otherwise noted, four vinyl biocarbon bioreactors were installed at regular intervals of 70 cm to 80 cm.

A multi-column-structure transparent film sheet photo bioreactor was fabricated based on the existing patent application (Application No. 10-2010-0005212) (Fig. 15).

The reactor was divided into seven uniformly spaced polyethylene films through thermal bonding, and seven columns were connected to each other. Three silicon tubes were connected to the upper door of each cylindrical unit for gas supply, gas outflow and sample collection.

In order to prevent precipitation of photosynthetic microorganisms, the lower part of each cylinder was made into a V shape, and a spherical sintered air diffuser was connected to the gas supply silicone tube.

3) Dry weight of photosynthetic microorganisms

The dry weight was measured using the principle of the method of measuring suspended solids. The photosynthetic microorganism culture was filtered with a filter and dried, and the weight difference between before and after drying was measured. The correlation between OD _660nm and KR-1 dry weight (g / L) is as follows.

Dry cell weight (g / L) = 0.2244 x OD _{660 nm}

Example 2: Comparison of Vinyl Sheet Type and Multicylindrical Structure Plate Transparent Film Photobioreactor

The production characteristics and the photosynthetic microbial productivity of the cylindrical sheet - type transparent film photobioreactor designed in the existing patent and the vinyl sheet type photobioreactor developed in this study were compared.

Table 1 shows fabrication and operation characteristics according to the structural difference of the vinyl sheet type and multi cylinder type flat plate type photobioreactor. In the case of gas diffuser, a cylindrical spherical photobioreactor used a general spherical sintered air diffuser, and a vinyl sheet type photobioreactor used a tube-shaped stainless steel porous metal membrane.

The multi-cylinder bioreactor is composed of seven space-separated cylinders, and sampling, gas supply, and gas discharge are performed through the top of each cylinder structure, so a considerable amount of the silicon tube is required.

On the other hand, the vinyl sheet type photobioreactor is composed of one space, and the entrance for supplying the gas or sampling the sample is located at the lower end of the reactor, so that the length of the silicon tube can be reduced to 1/8 of that of the cylindrical structure photobioreactor.

Also, the number of entrance and the number of silicon plugs is about half of that of the multi-cylinder structure. Also, the flowmeter required for controlling the gas supply rate has one vinyl sheet structure, which is considerably smaller than the case of the cylindrical structure (seven).

For photosynthetic microbial harvesting, the photocatalytic bioreactor is located at the top of the reactor, so the pump should be used. However, the vinyl sheet structure photobioreactor can be harvested simply by gravity without using the pump. .

Considering the total length of the silicon tube, the number of openings and silicone plugs, the number of gas flowmeters, and the use of a pump for harvesting the culture, the space-integrated vinyl sheet structure is more cost effective than the space- It is expected to be economically advantageous.

The difference is expected to be even larger, especially considering the large-scale culture of photosynthetic microorganisms (hectare scale).

Comparison of fabrication and operation characteristics according to the structure of plate type photobioreactor Item Plate-type transparent film photobioreactor Multi-cylinder structure * Vinyl sheet construction ** Culture volume (L) 28.5 16.5 Silicone tube usage
(cm) Sampling (inside reactor) 315 0 Sampling (outside the reactor) 70 10 Gas supply (inside reactor) 770 3 Gas supply (outside the reactor) 210 120 Gas discharge (outside the reactor) 630 100 Sum 1,995 233 Gas diffuser Sintered air diffuser Metal film (68 cm) Number of silicon stoppers used for doorway 7 3 Number of flowmeters 7 One How to harvest culture Using the pump Pump usage X

* Based on 7 cylinders

** Thermal bonding ratio 29%

The comparative experiments were carried out under natural conditions in an outdoor glasshouse for 147 hours. The room temperature in the greenhouse was 12.4 ~ 34.5 ℃ and the maximum light intensity was 489.2 μmol photons / m ² / s.

Fig. 16 shows the growth curves of KR-1 according to the photobioreactor structural difference (vinyl sheet structure vs. multi-cylinder structure). Relatively high cell growth rate was observed in a vinyl sheet type photobioreactor than in a multi-cylinder structure. The final bacterial concentration (absorbance) after 147 hours incubation was 3.1 times higher in the vinyl sheet structure than in the cylindrical structure (absorbance 1.6).

It is considered that this is due to the difference of light transmittance according to the structure of the photobioreactor. In the case of a multi - cylinder structure, the thickness of the reactor was 8 cm thick, whereas the thickness of the reactor was 5 cm in the case of the vinyl sheet structure.

That is, it is considered that the vinyl sheet type photobioreactor having a thinner reactor thickness has higher light transmittance than the multi-cylinder structured plate type photobioreactor and shows a relatively fast cell growth rate and a high final cell density.

On the other hand, the vinyl sheet type photobioreactor had a relatively small volume of 45% of the culture volume because of its wide thermal bonding area. However, due to the fast rate of growth rate, the final photosynthetic microbial biomass production was 5% higher (Figure 17).

Therefore, in terms of productivity and economical efficiency of the photosynthetic microbial biomass, the vinyl sheet type photobioreactor developed in this study is considered to be more advantageous than the conventional multi - cylinder structured plate type photobioreactor.

Example 3: Influence of the shape of thermally bonded surfaces

The polyethylene film has a characteristic that the bonding surface becomes opaque when thermally bonded. In this study, we investigated the effect of thermally bonded surface morphology on the growth of photosynthetic microorganisms in a vinyl sheet type photobioreactor. The shape of the thermocouple is (A) when the thermocouple is translucent, (B) when the aluminum foil is attached to prevent the light from penetrating the thermocouple, (C) (Fig. 18).

Cultivation was carried out in an outdoor greenhouse. The room temperature in the greenhouse ranged from 20 to 41 ° C, and the light intensity ranged from 0 to 875 μmol photons / m ² · s. Four photobioreactors were installed at regular intervals on an area of 0.56 m ² (70 cm * 80 cm).

The difference in the amount of light irradiation according to the shape of the thermal interface was measured at the midpoint between the first reactor and the second reactor in the morning, lunch, and evening (Fig. 19).

When the cell density was low at the early stage of culture (before 20 hours) due to the light reflection effect of aluminum (Foil) aluminum, the amount of light irradiation was slightly higher than that of the opaque case (opaque) The amount of light irradiation was similar to that of opaque surface or rather low.

In case of punching the thermal interface (Transparent), relatively high light dose was shown in the range of 20 to 70 hours. Thereafter, as the cell concentration increased, there was no significant difference between the other forms and light dose. This indicates that, at a certain cell concentration (OD 2.0 level) or less, the hole piercing on the heat bonding surface is more advantageous in terms of the light irradiation amount (see FIG. 20). It is expected that the effect will be further increased in a large-scale culture.

FIG. 20 shows the growth characteristics of photosynthetic microorganisms according to the shape of the thermally bonded surface. Transparent was the highest when the cell growth rate and final cell concentration were highest, followed by opaque (Opaque) and then with aluminum (Foil) on the thermocouple.

This suggests that the piercing of the holes in the thermal interface promotes the photosynthetic growth of photosynthetic microorganisms, especially in light of the amount of irradiation (70 hours before culture). The final mean OD _660nm was 2.9 when drilled and 10% higher than when the thermal _interface was opaque (2.6).

Example 4: Influence of thermal interface ratio

In a vinyl sheet type photobioreactor, the ratio of the thermal bonding area to the total film directly affects the incubation volume and thickness of the reactor. That is, as the thermal junction area increases, the thickness of the vinyl sheet type photobioreactor capable of wide-area connection is reduced, and ultimately, the culture volume of the photobioreactor capable of containing the culture liquid is reduced.

On the other hand, if the thickness of the reactor is reduced by the decrease of the heat-bonding area, the length of the light entering the reactor becomes longer due to the increase in the volume of the reactor. However, the photosynthetic microorganisms in the reactor can not receive sufficient light, which is disadvantageous in terms of the photosynthesis efficiency of the photosynthetic microorganisms do. In addition, if the volume increases as the thermal bonding area decreases, the durability of the vinyl reactor may be greatly affected.

FIG. 21 shows the correlation between the volume of the culture medium and the reactor thickness according to the thermal bonding ratio (24, 29, 36%). Cultivation was carried out in a room temperature incubator. The gas was supplied with VVM adjusted to 0.18 in consideration of the culture volume, and the reactor was operated under the same experimental conditions except for the culture volume.

FIG. 22 shows changes in the cell concentration of KR-1 according to the ratio of the thermal junction area. At the thermal bonding ratio of 29% and 36%, similar cell growth rate and final cell concentration were obtained. At the thermal bonding ratio of 24%, the cell growth rate and the final cell concentration were lower than the thermal bonding ratio of 29% and 36%.

This is probably due to the fact that the thickness of the photobioreactor becomes thicker and the light transmission decreases relatively as the thermal bonding ratio of semi-ellipses becomes lower. On the other hand, 24% of the cells with the largest volume of the culture medium had the highest 11.2 ~ 14.9 g cell / reactor (Figure 23).

When the cell productivity was calculated at 70 hours of incubation, the thermal bonding ratio of 24% was the best at 3.6 ~ 4.8 g cell / reactor / day. It seems to be beneficial in terms of culture volume. As the culture progressed, the dead zone was partially observed because the culture liquid was not circulated smoothly to the upper part of the semi-elliptical thermal junction. As the thermal bonding ratio increased, that is, as the number of semi-elliptical thermal junctions increased, A dead zone was formed (Fig. 24).

Dead zone formation is considered to have a great influence on the growth of photosynthetic microorganisms as a whole (especially the junction area of 36%). This indicates that the shape of the thermal joint greatly affects the performance of the vinyl sheet type photobioreactor. Therefore, it is considered necessary to improve the shape of the thermal bonding surface (for example, △ type instead of ∇ type) so as to prevent a dead zone.

Example 5: Influence of gas supply rate

The effect of the gas supply rate on the growth of KR-1 cells in a vinyl sheet type photobioreactor was investigated. Cultivation was carried out in a constant temperature incubator equipped with a fluorescent lamp. The thermal splice ratio and culture volume were 29% and 15 L, respectively. The gas supply rate was controlled in the range of 2.0 to 5.0 L / min (VVM 0.13 to 0.33).

The cell growth rate and the final cell concentration tended to increase as the gas supply rate increased in the gas supply rate range of 2.00 to 3.75 L / min (FIG. 25).

The optimum gas feed rate was observed at 0.25 VVM. At higher gas feed rates of 5.00 L / min (0.33 VVM), the cell growth rate and final cell concentration were lower than at 2.75 L / min. This suggests that excessive gas supply may cause shear stress in photosynthetic microorganisms, which may inhibit the growth of microorganisms.

Example 6 Influence of Thermal Junction Form

The influence of the thermal junction type (∇ type vs. Δ type) on the cell growth of KR-1 in a vinyl sheet type photobioreactor was investigated (FIG. 26). Cultivation was carried out in a constant temperature incubator equipped with a fluorescent lamp. The thermal splice ratio and culture volume were 29% and 15 L, respectively. The CO ₂ concentration of the supplied gas was 10% (v / v) and the feed rate was 3.75 L / min (0.25 VVM).

In the ∇ type control junction, the culture was proceeding as mentioned above, and the dead zone was partially observed because the culture liquid was not smoothly circulated to the upper part of the semi-elliptic thermal junction (see FIG. 24). On the other hand, no ruffled part was observed in the △ type thermal joint. The cell productivity (g cell / L / day) and the cell mass per reactor (g cell / reactor) of △ type thermal junctions were increased by 15% and 13%, respectively, compared to the control thermal junctions (Fig. 27).

Example 7 Effect of Reactor Length

Microalgae were cultured in 1.5, 2.0 and 2.5 m long bioreactors to compare the cell growth along the length of the reactor. 28 is a photo of a photobioreactor of each length. The culture volumes of the 1.5 m, 2.0 m and 2.5 m length reactors were 19 L, 25 L and 32 L, respectively (FIG. 29). 28 is a graph showing relative cell productivity based on a 2.0 m reactor of each reactor. The cell productivity and lipid productivity of the microalgae in the reactor per unit area increased with the length of the reactor. The productivity of the cells per unit volume, that is, the growth rate of the cells was similar regardless of the length of the reactor, but the productivity was improved due to an increase in the culture volume as the length increased. Relative bacterial productivity of 1.5 m and 2.5 m was 0.97 when the cell productivity per unit volume of the reactor of 2.0 m was regarded as 1. On the other hand, relative bacterial productivity per unit area was found to be 0.71 for 1.5 m and 1.25 for 2.5 m, which is proportional to the length of the reactor. In the case of the length up to 2.5 m, inhibition of cell growth was not observed by increasing the length of the reactor, and it is considered that the optimization of the length of the reactor would increase the culture volume and improve the productivity.

Example 8: Semi-continuous culture

In this experiment, a semi-continuous culture was carried out by recycling the culture solution to 10 ~ 50% without injecting new cells in a plate-type transparent film photobioreactor for 1155 hours (about 49 days). From the 1st to the 4th culture, the nitrate concentration was 1 mM, the nitrate concentration was 3 mM for the 5th culture, the nitrate concentration was 1 mM for the 6th culture and the nitrate concentration was 2 mM for the 7th culture. Only the natural light was used from the 1st to the 5th culture, and the 6th and 7th cultures were cultured under natural light conditions during the day and artificial light at night.

The shape of the thermal interface in the photobioreactor was circular, and the ratio of the thermal interface to the total area was 12%. The length of the reactor was 2 m, and the culture volume was 25 L. The temperature was 13.2 to 42.4 ° C and the maximum light intensity was 1370 μmol photons / m ² / s (Fig. 34). The initial nitrate concentration of the culture medium was adjusted to 1 to 3 mM. Gas was supplied by a gas mixture of CO ₂ and air to the CO ₂ concentration of 10% in 1-2 subculture at a flow rate of 3.25 L / min, 3 ~ 7 subculture, the flow rate of 4 L / min.

FIG. 31 shows changes in the cell concentration of KR-1 with time. The initial cell concentration and the final cell concentration tended to increase as the culture degree increased, even though the conditions from the first to the fourth culture were the same. The cell productivity decreased about 10 ~ 20% with increasing culture degree, but increased about 7% in the 4th culture compared to the 3rd culture. In the 5th culture, the initial nitrate concentration was 3 mM and the cell productivity was 0.12 g cell / L / day. In the 6th and 7th cultures irradiated with artificial light at night, the cell productivity was about 13% higher than that of the 7th culture, which was 2 mM at 0.17 g cell / L / day in 6th culture with nitrate concentration of 1 mM. Lipid contents were 433 ~ 443 mg FAME / g cell from the first to the third culture [Figure 32]. In the 4th culture, the lipid content was 478 mg FAME / g cell, which was about 7% higher than the 3rd culture. Lipid productivity was the highest at 4.34 L FAME / m ² / year in the primary culture (FIG. 33). And 3.44 L FAME / m ² / year in the ^second culture. In the 5th culture with an initial nitrate concentration of 3 mM, the lipid productivity was 4.13 L FAME / m ² / year, which was about 6% higher than that of the 4th culture. When artificial light was irradiated at night, it was 4.68 L FAME / m ² / year in the 6th culture with the initial nitrate concentration of 1 mM and 5.90 L FAME / m ² / year in the 7th culture with 2 mM. Although the cell productivity tended to decrease slightly as the cultivation degree increased as the culture was recycled, it is considered to be more influenced by external factors such as weather than semi-continuous cultivation. The photobioreactor used for semi-continuous cultivation was able to operate for a long time without damaging the reactor and contamination of the culture medium for 1155 hours.

According to the present invention configured as described above, a plurality of reaction sheets made of vinyl material can be arranged in a vertical shape to enable mass culture of the photosynthetic microorganisms and to reduce the installation and management cost, and the plurality of light- Thereby increasing the light transmittance and increasing the productivity.

Since a plurality of reaction sheets are arranged in series and connected through a connection portion, the culture liquid and the microorganism can be injected or discharged at once, and the photosynthetic microorganism can be discharged at a time.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is evident that many obvious changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention should therefore be construed in light of the claims set forth to cover many of such variations.

10: Reaction sheet
11: culture space 12:
13: Transmission hole 14: Insertion hole
15: Fixing hole
20: gas supply part
21: Supply piping 22: Bubble tube
23: pore 24: feed pump
30:
31: inlet / outlet port 32: exhaust pipe
33: Discharge pump
40: Support 50: Fixture
60: Outlet
61: Valve
70: Fixing device
80: Connection
81: connecting body 82: connecting groove
83: Valve
90: reflector
100: Thermal camera
101: Monitoring device

Claims (39)

A reaction sheet made of a vinyl material in which a culture space in which a photosynthetic microorganism and a culture medium are contained is formed in the inside, and front and rear surfaces are bonded by thermal bonding to form a plurality of transparent portions;
A gas supply unit for supplying carbon dioxide to the inside of the reaction sheet;
An inlet and an outlet for discharging oxygen produced by the photosynthesis action of the photosynthetic microorganism in the carbon dioxide supplied to the interior of the reaction sheet; And
And connecting portions formed on both sides of the reaction sheet so that the reaction sheets are connected in series or in parallel,
The transparent portion may be formed in any shape of a circle, an ellipse, a semi-circle, or a polygonal shape in which the photosynthetic microorganism does not become a droplet and a droplet is not formed on the top, and is arranged continuously or zigzag. Reactor.
The method according to claim 1,
Wherein the photosynthetic microorganism is at least one selected from the group consisting of microalgae, cynomolgus and photosynthetic bacteria.
The method according to claim 1,
The reaction sheet is made of a polyethylene (PE), a low density polyethylene (LDPE), a linear low density polyethylene (LLDPE), a high density polyethylene (HDPE), an oriented polypropylene (OPP), a polyethylene terephthalate and an unleaded polypropylene mixed film (CP), Nylon, Biaxially Oriented Nylon (ON), Untreated Nylon (CN), Polyacetal (POM), Polycarbonate (PC), Polyester, Polystyrene (PS) (PVP), Ethylene vinyl acetate copolymer (EVA), Polyethylene terephthalate (PET), Polypropylene (PP), Inflated Polypropylene (IPP) and Poly Phenylene oxide (PPO = PPE) or a material in which the above-mentioned materials are combined.
delete The method according to claim 1,
Wherein the transparent portion is capable of wide-area connection in which a through-hole is formed at the center.
The method of claim 5,
Wherein the through hole is formed in an area ratio of 50 to 90% of a total area ratio of the transparent portion.
The method according to claim 1,
Wherein the reaction sheets are composed of a plurality of reaction sheets, and the reaction sheets are spaced apart from each other by a predetermined distance and installed in parallel.
The method of claim 7,
Wherein the transmission portion of the plurality of reaction sheets is formed so as to be shifted so that the transmission portions of the respective reaction sheets do not coincide with each other in parallel.
The method according to claim 1,
And a wide-area connection is possible in which a reflection plate is installed at a position spaced apart from the reaction sheet at a predetermined interval to reflect light and reflect the light onto the reaction sheet.
The method according to claim 1,
The gas-
A supply pipe connected to the reaction sheet;
A bubble tube connected to the supply pipe and inserted into a lower side of the reaction sheet to form a plurality of pores to supply gas supplied from the supply pipe in the form of bubbles; And
And a supply pump installed at one side of the supply pipe to pump carbon dioxide into the reaction sheet.
The method of claim 10,
And the amount of gas supplied into the reaction sheet through the bubble tube is 0.01-0.3 VVM.
The method of claim 10,
Wherein the bubble tube is capable of wide-area connection in which a plurality of bubbles are inserted according to the size of the reaction sheet.
The method of claim 10,
Wherein the bubble tube has a smaller diameter along the longitudinal direction in a direction opposite to the supply tube side, and a pore interval of the bubble tube is gradually narrowed, thereby forming a wide-area connection.
The method of claim 10,
Wherein the bubble tube is capable of wide-area connection made of any one of stainless steel, polysulfone, polyethersulfone, polyvinylidene fluoride, Teflon, polyethylene, polypropylene, stainless steel, aluminum, ceramic and silicone.
The method of claim 10,
Wherein the pores formed in the bubble tube are capable of wide-area connection having a size of 0.01 to 300 um.
The method of claim 10,
Wherein the bubble tube is capable of wide-area connection in which a supply pipe and a supply pump are additionally provided according to the size of the reaction sheet.
The method according to claim 1,
The input /
An inlet and an outlet formed on one side of the reaction sheet for injecting the culture liquid and the photosynthetic microorganism and discharging oxygen generated during growth of the photosynthetic microorganism; And
And a discharge pipe having one end connected to the inlet and the other end extending to the outside,
18. The method of claim 17,
And a discharge pump installed on the discharge pipe for pumping oxygen to the outside of the discharge pipe.
The method according to claim 1,
Wherein the reaction sheet is fixed to a fixing device mounted on a floor by being coupled with a support on an upper end thereof.
The method according to claim 1,
And a fixing table for holding the shape of the reaction sheet is coupled to the lower end of the reaction sheet.
The method according to claim 1,
Wherein the reaction sheet has a discharge port for discharging the photosynthetic microorganism culture liquid inside the reaction sheet is formed on the side or bottom of the reaction sheet.
The method according to claim 1,
Wherein the transparent portion is formed at an area ratio of 5 to 35% of the total area of the reactive sheet.
The method according to claim 1,
Wherein the reaction sheet is capable of wide-area connection with a reflection plate provided below to reflect external light to a reaction sheet.
The method according to claim 1,
The connection part is made of the same material as the material of the reaction sheet and is formed into a pipe shape in which the reaction sheet is connected to the connection part, and the connection part on one side of the reaction sheet is connected to the connection part, And the other side connecting portion of the reaction sheet is formed as a groove-like insertion groove into which the connecting body can be inserted.
The method of claim 1 or 24,
Wherein the connection portion is provided with a valve to allow a wide area connection to prevent the microorganisms and the culture liquid in the reaction sheet from flowing out when the other reaction sheet is not bonded.
The method according to claim 1,
A thermal image camera capable of photographing a temperature change of the reaction sheets is installed at a predetermined height of a position spaced apart from the reaction sheet by a predetermined distance and the thermal image camera is connected to a monitoring device, A connectable vinyl sheet type photobioreactor.
A method for producing a photosynthetic microorganism comprising the steps of adding a photosynthetic microorganism to a photobioreactor according to any one of claims 1 to 3, and culturing the same.
delete Preparing a reaction sheet made of a vinyl material on which a plurality of permeation parts are formed by forming a culture space in which a photosynthetic microorganism and a culture liquid are contained,
A reaction sheet mounting step of arranging the reaction sheets in a vertical or horizontal form by supporting upper and lower portions of the reaction sheets by a support;
Connecting the reaction sheets through a connection portion;
Providing a gas supply unit for supplying carbon dioxide to a culture space of the reaction sheet; And
And an inlet and an outlet for discharging oxygen generated in the culture space of the reaction sheet,
Wherein the transparent portion is formed in one of a circular shape, an elliptical shape, a semi-circular shape, or a polygonal shape in which a droplet is not formed on the top so that the photosynthetic microorganism does not become a droplet, and is arranged continuously or zigzag.
delete 29. The method of claim 29,
Wherein the transparent portion is capable of wide-area connection in which a through-hole is formed at the center.
29. The method of claim 29,
The gas-
A supply pipe connected to the reaction sheet;
A bubble tube connected to the supply pipe and inserted into a lower side of the reaction sheet to form a plurality of pores to supply gas supplied from the supply pipe in the form of bubbles; And
And a supply pump installed at one side of the supply pipe for pumping carbon dioxide into the reaction sheet to supply the carbon dioxide into the reaction sheet.
33. The method of claim 32,
Wherein the bubble tube is capable of wide-area connection in which a plurality of bubbles are inserted according to the size of the reaction sheet.
29. The method of claim 29,
The input /
An inlet and an outlet formed on one side of the reaction sheet for injecting the culture liquid and the photosynthetic microorganism and discharging oxygen generated during growth of the photosynthetic microorganism; And
And a discharge pipe having one side connected to the inlet and the other side extending to the outside.
29. The method of claim 29,
The connection part is made of the same material as the material of the reaction sheet and is formed into a pipe shape in which the reaction sheet is connected to the connection part, and the connection part on one side of the reaction sheet is connected to the connection part, And the other side connecting portion of the reaction sheet is formed as a groove-like insertion groove into which the connecting body can be inserted.
29. The method of claim 29,
And a fixing table for holding the shape of the reaction sheet is coupled to the lower end of the reaction sheet.
29. The method of claim 29,
Wherein the reaction sheet is provided with a discharge port for discharging the photosynthetic microorganism culture liquid inside the reaction sheet on the side or bottom of the reaction sheet.
29. The method of claim 29,
Wherein the reaction sheet is installed at a lower portion of the reaction sheet at a predetermined interval to provide a reflection plate for reflecting light.
29. The method of claim 29,
A thermal image camera capable of photographing a temperature change of the reaction sheets is installed at a predetermined height of a position spaced apart from the reaction sheet by a predetermined distance and the thermal image camera is connected to a monitoring device, A method of installing a vinyl sheet type photobioreactor capable of being connected.

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